| translation in vitro of carnation mottle virus rna. regulatory function of the 3'-region. | | 1978 | 726253 |
| mapping and sequence analysis of the capsid protein gene of cowpea mottle virus. | twelve cdna clones were generated, covering approximately 95% of the cowpea mottle virus (cmev) genome from the 3' end to near the 5' end. the entire capsid protein sequence of 1,104 nucleotides was contained in two clones located near the 3' terminus. the codons represented 367 amino acids (m(r) 39,611). the postulated amino acid sequence of cmev capsid protein had 36% homology to turnip crinkle virus and 26% homology to carnation mottle virus in the arm and s domains, but western blots showed ... | 1992 | 1500274 |
| the complete nucleotide sequence of tobacco necrosis virus strain d. | the complete sequence of the rna genome of tobacco necrosis virus strain d (tnv-d) consisting of 3759 nucleotides has been determined. the positive strand contains five open reading frames (orfs). the 5'-proximal orf encodes a 22k protein terminating with an amber codon which may be read through to produce a 82k protein (p82). two small centrally located orfs each encode two out-of-frame 7k proteins (p7a and p7b). the 3'-proximal orf encodes the 29k coat protein (cp), the n terminus of which has ... | 1991 | 1856691 |
| genome structure of tobacco necrosis virus strain a. | an almost complete sequence of the rna genome of tobacco necrosis virus (tnv) strain a has been determined. the genome organization is very similar to that of carnation mottle virus (carmv) and turnip crinkle virus (tcv). the 5'-proximal open reading frame (orf) encodes a 23-kda protein and read-through of its amber codon into the second orf is presumably used for the translation of a 82-kda protein. the third large orf encodes the 30-kda coat protein. two small orfs are located upstream and one ... | 1990 | 2371773 |
| complete nucleotide sequence of the cucumber necrosis virus genome. | the complete nucleotide sequence of the cucumber necrosis virus (cnv) genome has been determined. the genome is 4701 nucleotides in length and contains five long open reading frames (orf). orf1 begins at the first aug codon at the 5' terminus and terminates at an amber codon. the predicted molecular weight of the polyprotein encoded by orf1 is 33 kilodaltons (kda). readthrough of the orf1 amber codon would yield a protein with a molecular weight of 92 kda. comparison of the amino acid sequence o ... | 1989 | 2705296 |
| the genome structure of turnip crinkle virus. | the nucleotide sequence of turnip crinkle virus (tcv) genomic rna has been determined from cdna clones representing most of the genome. segments were confirmed using dideoxynucleotide sequencing directly from viral rna, and the 3' terminal sequence was confirmed by chemical sequencing of end-labeled genomic rna. three open reading frames (orfs) have been identified by examination of the deduced amino acid sequences and by comparison with the orfs found in the genome of carnation mottle virus. or ... | 1989 | 2718381 |
| [the submolecular structure of the carnation mottle virus]. | the model of the tertiary structure of the cmtv subunit was suggested and the mode of its packaging into the capsid was determined on the basis of electron microscopy small-angle x-ray scattering and x-ray diffraction data. the i-ray diffraction data on the crystal of the native virion of carnation mottle virus were obtained on the automatic diffractometer supplied with two-dimensional detector. | 1989 | 2725538 |
| the complete nucleotide sequence of the maize chlorotic mottle virus genome. | the complete nucleotide sequence of the maize chlorotic mottle virus (mcmv) genome has been determined to be 4437 nucleotides. the viral genome has four long open reading frames (orfs) which could encode polypeptides of 31.6, 50, 8.9 and 25.1 kd. if the termination codons, for the polypeptides encoded by the 50 and 8.9 kd orfs are suppressed, readthrough products of 111 and 32.7 kd result. the 31.6 and 50 kd orfs overlap for nearly the entire length of the 31.6 kd orf. striking amino acid homolo ... | 1989 | 2726455 |
| the complete nucleotide sequence and genome organization of red clover necrotic mosaic virus rna-1. | the complete nucleotide sequence of red clover necrotic mosaic virus (rcnmv) rna-1 has been determined. rna-1 is 3889 nucleotides in length with a 5' terminal m7gpppa cap. the rna contains three large open reading frames (orfs): the 5' proximal orf, encoding a 27-kda polypeptide; the internal orf, coding for a 57-kda polypeptide; and the 3' terminal orf, encoding the 37-kda capsid protein. the sequence results confirm in vitro translation of 27-, 50-, and 37-kda products but do not account for t ... | 1989 | 2763465 |
| bean mild mosaic virus: genome and its translation products. | viral rna from purified preparations of bean mild mosaic virus (bmmv) was resolved into two rnas of mol. wt 1.5 x 10(6) daltons (r1) and 0.76 x 10(6) daltons (r2). complementary dna hybridizations, infectivity tests, and in-vitro translation studies established that r2 is a subgenomic rna derived from r1. in-vitro translation studies showed that the genomic rna (r1) coded for two major proteins of mol. wt. 40.5 kd and 25.7 kd. of these, the 40.5 kd protein was shown to be the capsid protein that ... | 1989 | 2763470 |
| sequence and organization of barley yellow dwarf virus genomic rna. | the nucleotide sequence of the genomic rna of barley yellow dwarf virus, pav serotype was determined, except for the 5'-terminal base, and its genome organization deduced. the 5,677 nucleotide genome contains five large open reading frames (orfs). the genes for the coat protein (1) and the putative viral rna-dependent rna polymerase were identified. the latter shows a striking degree of similarity to that of carnation mottle virus (carmv). by comparison with corona- and retrovirus rnas, it is pr ... | 1988 | 3399386 |
| structure and assembly of turnip crinkle virus. iv. analysis of the coat protein gene and implications of the subunit primary structure. | the structure of the turnip crinkle virus (tcv) coat protein and coat protein gene has been examined by cdna cloning, nucleotide sequencing and high-resolution mrna mapping. we have cloned a 1450-nucleotide cdna fragment, representing the 3' end of the tcv genome, using genomic rna polyadenylated in vitro as the reverse transcriptional template. nucleic acid sequence analysis reveals the presence of a 1053 nucleotide open reading frame capable of encoding a protein of 38,131 mr, identified as th ... | 1987 | 3612806 |
| nucleotide sequence and genome organization of carnation mottle virus rna. | the complete nucleotide sequence of carnation mottle genomic rna (4003 nucleotides) is presented. the sequence was determined for cloned cdna copies of viral rna containing over 99% of the sequence and was completed by direct sequence analysis of rna and cdna transcripts. the sequence contains two long open reading frames which together can account for observed translation products. one translation product would arise by suppression of an amber termination codon and the sequence raises the possi ... | 1985 | 3840587 |
| electrophoretic desorption of intact virus from immunoadsorbent. | a technique and apparatus are described for obtaining electrophoretic elution of virus from immunoadsorbent matrices. the method was applied to a well-known and easily purifiable virus, carnation mottle virus (carmv). the technique was applicable even when the starting material was diluted and the virus recovered was indistinguishable from the original. our aim was to avoid more drastic procedures that could damage virus structure (e.g. chaotropic chemicals, detergents, acidic ph's) and develop ... | 1985 | 3892232 |
| the effect of gamma radiation on carnation mottle virus in crude dianthus barbatus sap. | | 1970 | 4107101 |
| physical, chemical, and serological studies on carnation mottle virus. | | 1970 | 4991706 |
| an encapsidated, subgenomic messenger rna encodes the coat protein of carnation mottle virus. | the translation strategy of carnation mottle virus (carmv) in vitro has been generally assumed to involve internal initiation events on full-length, genomic rna (4.3 kb). we suggest that this is, at least in part, incorrect. encapsidated rna, fractionated on denaturing sucrose gradients, or total rna from carmv-infected leaves, fractionated under non-denaturing conditions, was translated in an mrna-dependent rabbit reticulocyte cell-free system. evidence for subgenomic rnas which encode a polype ... | 1984 | 6525451 |
| effect of ph and other conditions on immunosorbent electron microscopy of several plant viruses. | the efficiency of trapping, using immunosorbent electron microscopy, of particles of cucumber mosaic virus, lily symptomless virus, potato virus y, and carnation mottle virus, depended markedly on the ph of the virus extract. varying the ph of the serum had a smaller, though still significant effect. with potato virus y, the optimal ph of the virus extract varied with the host plant. it appears that for each virus and host a specific testing procedure is required to find the optimal trapping con ... | 1982 | 6752161 |
| use of highly conserved motifs in plant virus rna polymerases as the tags for specific detection of carmovirus-related rna-dependent rna polymerase genes. | two highly degenerate primers for sequence-specific amplification and cloning of a 510-nucleotide-long segment of rna-dependent rna-polymerase (rdrp) genes were selected and synthesized on the basis of available plant carmovirus-like viral rdrp sequences. these primers were shown to be efficient in pcr screening of different rdrp genes including those of carmoviruses, dianthoviruses, and tombusviruses. in particular, they were used for amplification, cloning, and sequencing of an rdrp gene fragm ... | 1995 | 7871745 |
| the atomic structure of carnation mottle virus capsid protein. | the structure of the carnation mottle virus (cmtv) capsid protein has been determined at 3.2 a resolution by the method of molecular replacement. three-dimensional data were collected from a small number of crystals (sp.g. i23, a = 382.6 a) using the synchrotron radiation with an image plate as detector. the coordinates of tomato bushy stunt virus (tbsv) were used as a searching model. refinement of the coordinates of 7,479 non-hydrogen atoms performed by the program xplor, has led to an r-facto ... | 1994 | 8307192 |
| relationship of the pelargonium flower break carmovirus (pfbv) coat protein gene with that of other carmoviruses. | the 3'-terminal 1500 nucleotides of the genome of pelargonium flower break carmovirus (pfbv) were sequenced from rt-pcr amplification products. one large orf was found, encoding a 345 amino acid protein of mr 37 kda, which corresponds to the coat protein, as confirmed by immunoprecipitation of products of in vitro transcription and translation. the sequence also included the putative promoter of the coat protein gene subgenomic rna, as well as its 5' and 3' untranslated regions. the pfbv coat pr ... | 1998 | 9787665 |
| in vivo detection, rna-binding properties and characterization of the rna-binding domain of the p7 putative movement protein from carnation mottle carmovirus (carmv). | biochemical and structural characterization studies on the p7 putative movement protein from a spanish isolate of carnation mottle carmovirus (carmv) have been conducted. the carmv p7 gene was fused to a sequence coding for a six-histidine tag and expressed in bacteria, allowing the purification of carmv p7 and the production of a specific antiserum. this antiserum led to the immunological identification of carmv p7 in infected leaf tissue from the experimental host chenopodium quinoa. putative ... | 1999 | 10069961 |
| characterization and in vitro translation analysis of pelargonium flower break virus. | pelargonium flower break virus (pfbv) is one of the common viruses in the glasshouses of western europe and has been assigned to the genus carmovirus. a spanish isolate obtained from nursery-grown pelargonium zonale plants (pfbv-m) has been characterized. the molecular weight of genomic rna and coat protein of pfbv-m were determined to be 1.36 x 10(6) (corresponding to approximately 4 kb) and 36,000, respectively. only genomic-size rna was encapsidated in pfbv virions; making necessary to purify ... | 1999 | 10486116 |
| the nucleotide sequence and genome organization of japanese iris necrotic ring virus, a new species in the genus carmovirus. | the genome of japanese iris necrotic ring virus (jinrv) consists of a positive-sense ssrna of 4014 nucleotides with six major open reading frames (orfs). a 5'-non-coding region of 31 nucleotides precedes the first initiation codon. like carnation mottle virus (carmv), the 5'-proximal three orfs encode a 26 kda protein (p26) and two readthrough proteins, i.e. an 85 kda putative rna replicase (p85) and a 99 kda protein (p99). the central orf encodes a small 8 kda protein (p8). the 3'-proximal orf ... | 2000 | 10795530 |
| simultaneous detection of five carnation viruses by non-isotopic molecular hybridization. | several viruses, which in some cases can cause severe losses, affect carnation plants. these viruses include carnation mottle virus, carnation etched ring virus (cerv), carnation vein mottle virus, carnation ringspot virus, carnation italian ringspot virus and carnation latent virus. a non-isotopic molecular hybridization was developed for the detection of these viruses in host plants and the sensitivity of the technique has been compared with enzyme-linked immunosorbent assay and bioassay metho ... | 1999 | 10894633 |
| structural properties of carnation mottle virus p7 movement protein and its rna-binding domain. | plant viral movement proteins (mps) participate actively in the intra- and intercellular movement of rna plant viruses to such an extent that mp dysfunction impairs viral infection. however, the molecular mechanism(s) of their interaction with cognate nucleic acids are not well understood, partly due to the lack of structural information. in this work, a protein dissection approach was used to gain information on the structural and rna-binding properties of this class of proteins, as exemplified ... | 2001 | 11279121 |
| molecular variability of twenty-one geographically distinct isolates of carnation mottle virus (carmv) and phylogenetic relationships within the tombusviridae family. | the complete nucleotide sequence of a spanish isolate of carnation mottle carmovirus (carmv) has been determined. phylogenetic analyses were carried out with the replicase, coat protein (cp) and the putative movement proteins (p7 and p9) of carmv with the homologous proteins of representative members of the different genera included within the family tombusviridae. these analyses revealed that phylogenetic trees obtained depended on the protein analyzed, and that the best correlation with taxono ... | 2001 | 11722023 |
| insertion and topology of a plant viral movement protein in the endoplasmic reticulum membrane. | virus-encoded movement proteins (mps) mediate cell-to-cell spread of viral rna through plant membranous intercellular connections, the plasmodesmata. the molecular pathway by which mps interact with viral genomes and target plasmodesmata channels is largely unknown. the 9-kda mp from carnation mottle carmovirus (carmv) contains two potential transmembrane domains. to explore the possibility that this protein is in fact an intrinsic membrane protein, we have investigated its insertion into the en ... | 2002 | 11976343 |
| spatio-temporal analysis of the rnas, coat and movement (p7) proteins of carnation mottle virus in chenopodium quinoa plants. | time-course and in situ hybridization analyses were used to study the spatio-temporal distribution of carnation mottle virus (carmv) in chenopodium quinoa plants. genomic and subgenomic rnas of plus polarity accumulated linearly with time, whereas the corresponding minus strands reached a peak during infection in inoculated leaves. analyses of serial tissue sections showed that plus polarity strands were localized throughout the infection area, whereas minus strands were localized at the borders ... | 2003 | 12604827 |
| complete nucleotide sequence and genome organization of pelargonium flower break virus. | the complete nucleotide sequence of pelargonium flower break virus (pfbv) has been determined. the genomic rna is 3923 nucleotides (nt) long and contains five open reading frames (orfs). the 5'-proximal orf encodes a 27 kda protein (p27) and terminates with an amber codon which may be read-through into an in-frame p56 orf to generate a 86 kda protein (p86) containing the viral rna dependent-rna polymerase motifs. two small orfs, located in the central part of the viral genome, encode polypeptide ... | 2004 | 14991450 |
| structure of cowpea mottle virus: a consensus in the genus carmovirus. | cowpea mottle virus (cpmov) is a t = 3 virus that belongs to carmovirus genus of the tombusviridae family. here, we report the crystal structure of cpmov determined to a resolution of 7.0 angstroms. the structures and sequences of three carmoviruses, cpmov, turnip crinkle virus (tcv), and carnation mottle virus (carmv) have been compared to tbsv from the tombusvirus genus. cpmov, tcv, and carmv all have a deletion in betac strand in the s domain relative to tbsv that may be distinctive to the ge ... | 2004 | 15051394 |
| transient structural ordering of the rna-binding domain of carnation mottle virus p7 movement protein modulates nucleic acid binding. | plant viral movement proteins bind to rna and participate in the intra- and intercellular movement of the rnas from plant viruses. however, the role and magnitude of the conformational changes associated with the formation of rna-protein complexes are not yet defined. here we describe studies on the relevance of a preexisting nascent alpha-helix at the c terminus of the rna-binding domain of p7, a movement protein from carnation mottle virus, to rna binding. synthetic peptide analogues and singl ... | 2005 | 16003802 |
| rna-binding properties and membrane insertion of melon necrotic spot virus (mnsv) double gene block movement proteins. | advances in structural and biochemical properties of carmovirus movement proteins (mps) have only been obtained in p7 and p9 from carnation mottle virus (carmv). alignment of carmovirus mps revealed a low conservation of amino acid identity but interestingly, similarity was elevated in regions associated with the functional secondary structure elements reported for carmv which were conserved in all studied proteins. nevertheless, some differential features in relation with carmv mps were identif ... | 2006 | 16950492 |
| complete nucleotide sequence of nootka lupine vein-clearing virus. | the complete genome sequence of nootka lupine vein-clearing virus (nlvcv) was determined to be 4,172 nucleotides in length containing four open reading frames (orfs) with a similar genetic organization of virus species in the genus carmovirus, family tombusviridae. the order and gene product size, starting from the 5'-proximal orf consisted of: (1) polymerase/replicase gene, orf1 (p27) and orf1rt (readthrough) (p87), (2) movement proteins orf2 (p7) and orf3 (p9), and, (3) the 3'-proximal coat pr ... | 2007 | 17657600 |
| the structure of melon necrotic spot virus determined at 2.8 a resolution. | the structure of melon necrotic spot virus (mnsv) was determined at 2.8 a resolution. although mnsv is classified into the genus carmovirus of the family tombusviridae, the three-dimensional structure of mnsv showed a higher degree of similarity to tomato bushy stunt virus (tbsv), which belongs to the genus tombusvirus, than to carnation mottle virus (cmtv), turnip crinkle virus (tcv) or cowpea mottle virus (cpmtv) from the genus carmovirus. thus, the classification of the family tombusviridae a ... | 2008 | 18097092 |
| synthesis and characterization of dna complementary to carnation mottle virus rna. | dna complementary to carnation mottle virus-rna (carmv rna) was synthesized using the reverse transcriptase of avian myeloblastosis virus (amv) as enzyme, and an hydrolysate of calf thymus dna as primer. after purification steps that included self-annealing and hydroxyapatite chromatography, the dna transcript was more than 99% sensitive to s1 nuclease and hybridized to at least 92% of the carmv-rna sequences, but not to heterologous rna. therefore, the purified transcript was single stranded an ... | 1980 | 18631672 |
| complementary dna cloning and analysis of carnation mottle virus rna. | a 3850-base pair (bp) cdna fragment, representing most of the carnation mottle virus (carmv) genome, was molecularly cloned in escherichia coli using a modification of the plasmid-primer method of okayama and berg (h. okayama and p. berg (1982), mol. cell. biol. 2, 161-170). poly(dt)-tailed puc8 was used to prime cdna synthesis from polyadenylated carmv rna template. oligo(dc) was added to the cdna 3' end, and second-strand synthesis was specifically primed using an oligo(dg)-tailed linker fragm ... | 1984 | 18639828 |
| characterization of the cell-free translation products of carnation mottle virus genomic and subgenomic rnas. | the in vitro translation products of carnation mottle virus (carmv) genomic and subgenomic rnas were analysed using a rabbit reticulocyte lysate system. viral rnas directed synthesis of three main polypeptides, p80, p40, and p34. p40, which was the predominant product using unfractionated virion rna as template, was identified as coat protein based on electrophoretic mobility through sds-polyacrylamide gels and immunoprecipitation with anti-carmv serum. upon size-fractionation of virion rnas by ... | 1985 | 18640513 |
| high resolution mapping of carnation mottle virus-associated rnas. | the genomic coordinates from which carnation mottle virus (carmv) subgenomic rna 5'-ends originate have been determined by high resolution s, nuclease protection and primer-extension mapping techniques. the two, 3'-proximal subgenomic rnas initiate at nucleotides 2532 and 2315 (counting from the genome 5' terminus). these rnas contain 1472 and 1689 nucleotides, respectively. there is substantial nucleotide sequence homology surrounding the transcriptional start sites of the two subgenomic rnas. ... | 1986 | 18640617 |
| biological and molecular characterization of a novel carmovirus isolated from angelonia. | abstract a new carmovirus was isolated from angelonia plants (angelonia angustifolia), with flower break and mild foliar symptoms, grown in the united states and israel. the virus, for which the name angelonia flower break virus (anfbv) is proposed, has isometric particles, approximately 30 nm in diameter. the experimental host range was limited to nicotiana species, schizanthus pinnatus, myosotis sylvatica, phlox drummondii, and digitalis purpurea. virions were isolated from systemically infect ... | 2006 | 18944305 |
| [development of immunochromatographic test systems for express detection of plant viruses]. | express immunochromatographic test-strip assays were developed for detection of five plant viruses varying in shape and size of virions: spherical carnation mottle virus, bean mild mosaic virus, rod-shaped tobacco mosaic virus, and filamentous potato viruses x and y. multimembrane composites (test strips) with immobilized polyclonal antibodies against viruses and colloidal gold-conjugated antibodies were used for the analysis. the immunochromatographic test strips were shown to enable the detect ... | 2009 | 19382712 |
| preliminary x-ray data analysis of crystalline hibiscus chlorotic ringspot virus. | hibiscus chlorotic ringspot virus (hcrsv) is a positive-sense monopartite single-stranded rna virus that belongs to the carmovirus genus of the tombusviridae family, which includes carnation mottle virus (carmv). the hcrsv virion has a 30 nm diameter icosahedral capsid with t = 3 quasi-symmetry containing 180 copies of a 38 kda coat protein (cp) and encapsidates a full-length 3.9 kb genomic rna. authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium ... | 2009 | 19478438 |
| carnation mottle virus, an important viral agent infecting carnation cut-flower crops in mahallat of iran. | one of the most important cut-flower crops grown worldwide on commercial scale is carnation (dianthus caryophyllus l.). it's the main production of mahallat where is one of the most important ornamental plants production centers of iran. infection of carnation with pathogens like viral agents causes economic losses in carnation cut-flower crop. one of the viral agents of this flower is carnation mottle virus (carmv) which is the type member of genus carmovirus and belongs to the tombusviridae fa ... | 2009 | 20222573 |
| analysis of the solvent accessibility of cysteine residues on maize rayado fino virus virus-like particles produced in nicotiana benthamiana plants and cross-linking of peptides to vlps. | mimicking and exploiting virus properties and physicochemical and physical characteristics holds promise to provide solutions to some of the world's most pressing challenges. the sheer range and types of viruses coupled with their intriguing properties potentially give endless opportunities for applications in virus-based technologies. viruses have the ability to self- assemble into particles with discrete shape and size, specificity of symmetry, polyvalence, and stable properties under a wide r ... | 2013 | 23439009 |
| characterization of a new carmovirus isolated from an adonis plant. | an isometric virus was isolated from a cultivated adonis plant (a. ramosa). the purified virus particle is 28 nm in diameter and is composed of a single coat protein and a single rna genome of 3,991 nucleotides. sequence analysis showed that the virus is closely related to carnation mottle virus. the virus was used to mechanically infect healthy a. ramosa plants, resulting in mosaic and leaf curl symptoms; however, attempts to inoculate carnation plants did not result in infection. we propose th ... | 2017 | 27738845 |
| uncoating mechanism of carnation mottle virus revealed by cryo-em single particle analysis. | genome uncoating is a prerequisite for the successful infection of plant viruses in host plants. thus far, little is known about the genome uncoating of the carnation mottle virus (carmv). here, we obtained two reconstructions of carmv at ph7 in the presence (ca-ph7) and absence (edta-ph7) of calcium ions by cryo-em single particle analysis, which achieved 6.4 å and 8 å resolutions respectively. our results showed that chelation of the calcium ions under edta-ph7 resulted in reduced interaction ... | 2015 | 26442593 |
| complete genome sequence of a carnation mottle virus infecting hop plants. | the carnation mottle virus (carmv) is a single positive-strand rna virus belonging to the genus carmovirus. the major natural host for carmv is the carnation. in this study, using transcriptome data, we provide for the first time a nearly complete genome sequence of carmv infecting hop plants. | 2015 | 25977420 |