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characterization of two new serotypes of san miguel sea lion virus.two new virus isolates, one from a california sea lion (zalophus californianus californianus) and the other from a northern fur seal (callorhinus ursinus) were partially characterized. their physicochemical characteristics were similar to those of vesicular exanthema of swine virus (vesv) and san miguel sea lion virus (smsv). the virion morphology was in both instances typically calicivirus. on the basis of this and the serum cross-neutralization testing, these isolates were classed as two new t ...197767101
san miguel sea lion virus fed to mink and pigs.mink became infected with san miguel sea lion virus when fed ground meat from seal carcasses showing vesicular-like lesions in the skin. the mink also contracted the infection when they were fed san miguel sea lion virus infected pig meat or cell culture propagated virus. san miguel sea lion virus infection in mink was inapparent but the virus was isolated from blood and rectal swabs. pigs treated similarly with the same virus preparations given to mink developed a severe vesicular disease syndr ...197896925
a new calicivirus isolated from a marine mammal.a new serotype of calicivirus, designated as san miguel sea lion virus type 6 (smsv-6), was isolated from vesicular lesions on the flipper of a california sea lion pup. serologic studies show that smsv-6 neutralizing antibodies (sn) occur frequently among california sea lions and occasionally among northern fur seals. feral swine, 1- to 6-week elephant seal pups and grey whales tested negative for smsv-6 antibody.1979227346
caliciviruses infecting monkeys and possibly man.caliciviruses have, for the 1st time, been shown experimentally to infect a primate. twenty-four hours after being inoculated with san miguel sea lion virus (smsv), an african green monkey developed a febrile response and vesicular lesions at injection sites. virus was recovered from lesion material 96 hours later and from the stool at 48 hours. possible human infection with smsv was indicated by serologic evidence. three persons working with 4 distinct serotypes of smsv developed neutralizing a ...1978415648
host range comparisons of five serotypes of caliciviruses.two new serotypes of san miguel sea lion virus (smsv), designated smsv-4 and smsv-5, were studied in vivo and in vitro. the host cell spectrums were compared with smsv-1, smsv-2, and vesicular exanthema of swine virus type a-48. based on the result of these broad host spectrums, a numerical scoring system was devised for ranking each virus on the basis of its potential for infecting terrestrial mammals, including the important domestic species.1977556918
rna synthesized in calicivirus-infected cells is atypical of picornaviruses.rna labeled with [3h]uridine from vero cells infected with san miguel sea lion virus in the presence of actinomycin d was analyzed by glycerol density gradient sedimentation and polyacrylamide gel electrophoresis. the predominant single-stranded rna (36s, 2.6 x 10(6) molecular weight) was genome size. there was also a prominent 22s, 1.1 x 10(6)-molecular weight, single-stranded component and one or more double-stranded or partially double-stranded classes. replicative forms, sedimenting at 18s, ...1977559106
relationship of san miguel sea lion virus to other members of the calicivirus group.san miguel sea lion virus (smsv) is indistinguishable from vesicular exanthema virus (vev) and feline calicivirus (fcv) in its morphology and in possessing a single capsid polypeptide with a molecular weight of approximately 65 x 10(3). neutralization tests readily differentiate the three viruses, but immunodiffusion tests show that smsv is closely related serologically to vev but not to fcv. although the rnas of the three caliciviruses have similar base compositions, homology tests show that sm ...1978564894
immunoelectron microscopic comparisons of caliciviruses.using immunoelectron microscopy, 9 serotypes of vesicular exanthema of swine virus (vesv) were compared with 5 serotypes of san miguel sea lion virus and 7 additional calicivirus isolates from marine animals. in addition, swine caliciviruses and marine caliciviruses were compared with the vaccinal strain of feline calicivirus (fcv) f-9. of 9 vesv types, 8 showed common antigenicity with san miguel sea lion virus. of 9 vesv types, 2 showed common antigenicity with fcv f-9. all 12 marine calicivir ...1978567950
comparative resistance of san miguel sea lion virus and vesicular exanthema of swine virus to chemical disinfectants.two similar calici agents, san miguel sea lion virus (smsv) and vesicular exanthema of swine virus (vesv) are susceptible to the virucidal activity of disinfectants of differing formulation. ten of 12 compounds were effective against six log10 plaque forming units (pfu) of smsv in a 2-min exposure at 4, 25 and 37 degrees c. however, only seven of these 10 smsv-positive compounds inactivated vesv under the same conditions of temperature and time. two compounds were not effective against smsv in a ...1978568301
calicivirus intracellular rna: fractionation of 18-22 s rna and lack of typical 5'-methylated caps on 36 s and 22 s san miguel sea lion virus rnas. 1979571647
isolation of san miguel sea lion virus from samples of an animal food product produced from northern fur seal (callorhinus ursinus) carcasses.a virus was isolated from california sea lions (zalophus californianus californianus) and northern fur seals (callorhinus ursinus) in 1972. it was later named san miguel sea lion virus (smsv). state and federal livestock disease control agencies became concerned, because smsv was found to be indistinguishable from vesicular exanthema of swine virus and to cause (in laboratory trials) clinical signs in swine similar to those produced by vesicular exanthema of swine virus. ground carcasses of nort ...1978629435
prevalence of vesicular exanthema of swine antibodies among feral mammals associated with the southern california coastal zones.serum-neutralizing antibodies to both vesicular exanthema of swine virus (vesv) and san miguel sea lion virus (smsv) were found in a number of animal species having an association with the southern california coastal zones. california sea lions (zalophus californianus) had antibodies to 9 vesv types (a48, c52, d53, e54, f54, g55, i55, j56, and k56). fur seals (callorhinus ursinus) and elephant seal pups (mirounga angustirostris) were tested for antibodies to 6 vesv types and all were negative. c ...1978629463
assay of antibodies to caliciviruses by radioimmune precipitation using staphylococcal protein a as igg adsorbent.a radioimmune assay method designated st-rip using a staphylococcal igg adsorbent, which potentially has broad applications to viral (and nonviral) antigen-antibody systems, was applied to detection of calicivirus antibodies. purified 125i-labeled virions of san miguel sea lion virus serotypes 4 (smsv-4) and 5 (smsv 5) were incubated with sera; the immune complexes were reacted with an immunoadsorbent, formaldehyde-fixed staphylococci (staphylococcus aureus protein a producer, strain cowan i), a ...1978678121
vesicular exanthema of swine.vesicular exanthema of swine (ves) was first recognized in 1932. at the time, eradication measures and, later, quarantine procedures were instituted and extension of the disease to surrounding farms appeared to have been prevented. between 1932 and 1936, however, seemingly unrelated epizootics continued among swine herds being fed raw garbage. in 1936, ves disappeared only to reappear in 1939. the disease was contained within california until 1952, at which time it spread to all the major swine ...1976786969
vesicular exanthema of swine and san miguel sea lion virus.san miguel sea lion virus (smsv), recently isolated from marine mammals, and vesicular exanthema of swine virus (vesv), which caused epizootics of vesicular exanthema of swine (ves) over a period of 24 years (1932 to 1956), may be the same virus. this finding is of particular interest because the source of the original ves epizootic was never identified, swine were the only known natural host of vesv, and vesv was thought to have been eradicated. the smsv has been shown to be enzootic in 2 speci ...1976786970
premature parturition in the california sea lion.twenty percent of the california sea lion pups born on san miguel island die due to premature parturition. specimens collected from premature-partus animals resulted in recovery of a virus, san miguel sea lion virus, indistinguishable from vesicular exanthema of swine virus, and leptospira pomona from some of the premature cows and pups. the age range of 10 females delivering healthy pups in june was 10-14 years. with one exception, the ages in 10 aborting females was 6-8 years. the p,p'-dde le ...1976815567
single major polypeptide of a calicivirus: characterization by polyacrylamide gel electrophoresis and stabilization of virions by cross-linking with dimethyl suberimidate.a calicivirus, san miguel sea lion virus serotype 4, isolate 15ft, externally labelled with 125i, was shown by gel electrophoresis to possess a single major polypeptide. the polypeptide migrated anomalously upon electrophoresis in two sodium dodecyl sulfate (sds) systems: more slowly than bovine serum albumin in a continuous phosphate-buffered system and more rapidly than bovine serum albumin in a discontinuous system. estimated molecular weights in the two systems were approximately 71,000 and ...1976972435
amino acid composition of three immunological types of a calicivirus, san miguel sea lion virus. 1976986102
haemorrhagic disease of lagomorphs: evidence for a calicivirus.studies on the aetiological agents of rabbit haemorrhagic disease (rhd) and european brown hare syndrome show that the viruses responsible for these infections can be placed in the family caliciviridae. established members of this group are vesicular exanthema virus (prototype), san miguel sea lion virus and feline calcivirus. the human hepatitis e virus and the norwalk agent may soon be included. the rhd virus genome consists of a positive stranded rna molecule composed of 7437 nucleotides. a m ...19921282757
nucleotide sequence of the capsid protein gene of two serotypes of san miguel sea lion virus: identification of conserved and non-conserved amino acid sequences among calicivirus capsid proteins.the san miguel sea lion viruses, members of the calicivirus family, are closely related to the vesicular disease of swine viruses which can cause severe disease in swine. in order to begin the molecular characterization of these viruses, the nucleotide sequence of the capsid protein gene of two san miguel sea lion viruses (smsv), serotypes 1 and 4, was determined. the coding sequences for the capsid precursor protein were located within the 3' terminal 2620 bases of the genomic rnas of both viru ...19921529644
new marine calicivirus serotype infective for swine.a new serotype of calicivirus was isolated from california sea lions (zalophus californianus) with severe vesicular disease. neutralizing antibodies were found in 27 of 82 (32.9%) serum samples from california sea lions and in 15 of 146 (10.3%) serum samples from steller sea lions (eumetopias jubatus) tested. the seropositive animals were widely dispersed along the margins of the eastern pacific basin, from the bering sea to the santa barbara channel. seropositive samples were found from as earl ...19902167030
first isolation of a calicivirus from the steller sea lion (eumetopias jubatus).a calicivirus was isolated from the rectum of a steller sea lion (eumetopias jubatus) pup on rogue reef, off the southern oregon coast. based on the results of neutralization tests with specific typing antisera, the isolate was identified as san miguel sea lion virus serotype 6 (smsv-6). blood obtained from nine of 37 pups (24%) during virus sample collection procedures had specific neutralizing antibodies to smsv-6. the isolation of smsv-6 from a steller sea lion represents, to our knowledge, t ...19873682080
antibodies to marine caliciviruses in the pacific walrus (odobenus rosmarus divergens illiger).sera from 155 pacific walruses (odobenus rosmarus divergens illiger), sampled in the chukchi sea during the summer of 1983, were tested for serum neutralizing (sn) antibodies to six marine calicivirus serotypes. serotypes tested included san miguel sea lion virus (smsv) types 1, 5, 8, and 10, previously isolated from northern fur seals (callorhinus ursinus linné) in the bering sea; walrus calicivirus (wcv), previously isolated from walrus feces collected off sea ice in the chukchi sea; and tilla ...19863712641
antibodies to marine caliciviruses in the steller sea lion (eumetopias jubatus schreber).sera from 145 steller sea lions (76 adults, three subadults, 37 pups, and 29 fetuses) were tested for neutralizing antibodies to nine marine calicivirus serotypes. antibodies were found to san miguel sea lion virus (smsv) types 1, 5, 6, 7, 8, 10 and 13, and to tillamook (bovine) calicivirus, but no antibodies were found to the walrus calicivirus. titers (microtiter neutralization assay) ranged from 1:20 to 1:320, with many positive reactions at the higher dilutions (greater than or equal to 1:80 ...19873820427
san miguel sea lion virus isolation, preliminary characterization and relationship to vesicular exanthema of swine virus. 19734583480
naturally occurring picornavirus infection of domestic mink.the isolation and preliminary characterization of a virus from domestic mink- are reported. the virus was tentatively classified as a member of the family picornaviridae on the basis of its physicochemical properties. the mink virus was not neutralized by antiserum to some known members of the calicivirus genus, which included the nine serotypes of vesicular exanthema of swine virus, ten serotypes of san miguel sea lion virus and feline calicivirus. seroepidemiological studies indicated that the ...19806256041
preliminary investigation of a possible lung worm (parafilaroides decorus), fish (girella nigricans), and marine mammal (callorhinus ursinus) cycle for san miguel sea lion virus type 5.colostrum-deprived neonatal northern fur seal pups (callorhinus ursinus) were exposed to san miguel sea lion virus type 5 (smsv-5) by feeding them fish (girella nigricans) infected with virus or fish infected with both the sea lion lung worm larvae (parafilaroides decorus) and virus. virus infection was demonstrated in 8 of 9 pups, and 1 of these developed a vesicular lesion on the flipper. in this sequence, p decorus larvae exposed to smsv-5 were fed to g nigricans held at 15 c in a salt water ...19806259973
calcivirus (smsv-5) infection in experimentally inoculated opaleye fish (girella nigricans).at 15 degrees c, san miguel sea lion virus infected fish (girella nigricans), producing 10(7).6 tcid50 per gram of spleen, replicated in vero cells (10(8) tcid50/gm) and retained viability after 14 days exposure to salt water (10(5) tcid50/ml dropped to 10(2).19816260062
experimental vesicular exanthema of swine virus and san miguel sea lion virus infection in phocid seals. 19826283712
vesicular exanthema of swine and san miguel sea lion virus: experimental and field studies in otarid seals, feeding trials in swine.the naturally occurring disease caused by san miguel sea lion virus in fur seals was characterized by small fluid-filled vesicles 1 to 25 mm in diameter on the nonhaired portions of the flippers. early epithelial lesions contained multifocal sites of cell lysis. the resultant microvesicles enlarged and coalesced, forming grossly visible macrovesicles. mature vesicles progressed to involve all layers of the epithelium but did not involve the underlying dermis. intradermal inoculation of vesicular ...19826283713
the pathogenesis of vesicular exanthema of swine virus and san miguel sea lion virus in swine.vesicular exanthema of swine virus type a48 or san miguel sea lion virus type 2, when inoculated intradermally into swine, resulted in fluid-filled vesicles at the sites of inoculation in the snout, coronary band, and tongue. pigs that developed vesicles also had fevers. secondary vesicle formation varied, depending on virus serotype. viremia was found in one pig infected with san miguel sea lion virus five days after infection. virus was recovered from nasal-oral passages for up to five days af ...19826283714
detection of swine caliciviruses by indirect immunofluorescence.the indirect immunofluorescence test is a rapid method for detecting the presence of vesicular exanthema of swine virus or san miguel sea lion virus in cell culture. a serological relationship exists between vesicular exanthema of swine virus and san miguel sea lion virus, as shown by the fluorescence-positive reactions between swine antisera to vesicular exanthema of swine virus a48 and san miguel sea lion virus type 5 and cell cultures infected with san miguel sea lion virus types 1, 2, 3, 4 a ...19806994862
isolation and identification of five new serotypes of calicivirus from marine mammals.five new serotypes of calicivirus have been isolated from marine mammals. san miguel sea lion virus (smsv)-8 and smsv-10 were recovered from vesicular lesions on the flippers of northern fur seals in the pribilof islands of alaska. serotype smsv-9 was isolated from a sea lion in southern california, and smsv-11 was isolated from 2 northern fur seal pups in southern california. serotype smsv-12 was also isolated in southern california from sea lion and fur seal pups.19817332133
a protein, vpg, covalently linked to 36s calicivirus rna.proteins associated with 36s virus rna from vero cells infected with san miguel sea lion virus, type 2 (smsv-2), were labelled with 125i. one protein, vpg, remained linked to rna when subjected to deproteinization techniques. vpg labelled wtih 32p was observed on 36s rna from purified virions; the quantity of label was compatible with two phosphates per genome. the estimated mol. wt. of smsv-2 vpg was 15 000.19807365464
calicivirus pathogenic for swine: a new serotype isolated from opaleye girella nigricans, an ocean fish.a new calicivirus, designated san miguel sea lion virus type 7 (smsv-7), was isolated from fish and produced a disease condition identical to vesicular exanthema in experimentally infected swine. serotype smsv-7 was also isolated from four elephant seals and one sea lion trematode, whereas a second calicivirus serotype isolated from fish proved to be smsv-6.19807403862
isolation of caliciviruses from skunks that are antigenically and genotypically related to san miguel sea lion virus.caliciviruses were isolated from feces of skunks imported from the north central united states to canada. virus isolation was accomplished using adenovirus-transformed human kidney (293) cells, swine testes and vero cells. plaque size variants were presented, but there was no apparent difference in virus morphology by negative stain or immune electron microscopy. pigs infected with skunk calicivirus had a slightly elevated body temperature at 3 days postinfection. although the infected animals s ...19957483817
analysis of the serologic relationship among san miguel sea lion virus and vesicular exanthema of swine virus isolates. application of the western blot assay for detection of antibodies in swine sera to these virus types.caliciviruses are positive-sense single-stranded rna viruses with a single capsid protein. the serotypes of the marine mammal calicivirus, san miguel sea lion virus (smsv), are antigenically related to vesicular exanthema of swine virus (vesv) and are potentially hazardous to swine. western blot assays using purified smsv serotypes 1 and 4 were used to further examine the serologic relationship among smsv and vesv isolates. with the exception of smsv 8 and smsv 12, rabbit polyclonal antisera gen ...19957619900
predicted stem-loop structures and variation in nucleotide sequence of 3' noncoding regions among animal calicivirus genomes.caliciviruses are nonenveloped with a polyadenylated genome of approximately 7.6 kb and a single capsid protein. the "rna fold" computer program was used to analyze 3'-terminal noncoding sequences of five feline calicivirus (fcv), rabbit hemorrhagic disease virus (rhdv), and two san miguel sea lion virus (smsv) isolates. the fcv 3'-terminal sequences are 40-46 nucleotides in length and 72-91% similar. the fcv sequences were predicted to contain two possible duplex structures and one stem-loop st ...19947975270
early interaction of feline calicivirus with cells in culture.the kinetics and biochemical properties of feline calicivirus (fcv) attachment to crandell-reese feline kidney cells were determined. maximum binding was observed at ph 6.5. cells in suspension at 4 degrees c bound virus more efficiently than cells in monolayers at 4 degrees c or 37 degrees c. high initial binding rate was observed in monolayers or cells in suspension and proceeded to a maximum at 90 min, although half maximal binding was observed as early as 15 min. binding was specific and com ...19948002786
viral serologic survey of bowhead whales in alaska.serum samples from 21 of 36 eskimo harvested bowhead whales (balaena mysticetus) were positive by virus neutralization (50% endpoint titer > or = 1:28 and/or 100% endpoint titer > or = 1:20) for antibodies to at least one virus serotype from the calicivirus family, vesicular exanthema of swine virus (vesv) and san miguel sea lion virus (smsv). many animals were positive to more than one serotype when using the spearman-karber (s-k) method for calculating antibody titers. the most common serotype ...19989476224
in vitro isolation and characterization of a calicivirus causing a vesicular disease of the hands and feet.we report that a calicivirus of oceanic origin, san miguel sea lion virus serotype 5 (smsv-5), is a human pathogen. this biotype was isolated originally from blisters on the flippers of northern fur seals (callorhinus ursinus) and replicates readily in primate and human cell lines. it infects a phylogenetically diverse array of hosts (poikilotherms to primates) and induces type-specific neutralizing antibodies in exposed humans. group antibody against a pooled antigen of smsv-5 and two other ser ...19989502467
the capsid protein of vesicular exanthema of swine virus serotype a48: relationship to the capsid protein of other animal caliciviruses.vesicular exanthema of swine virus (vesv), the prototype calicivirus, is the etiologic agent of the porcine disease vesicular exanthema of swine (ves). ves is characterized by vesicle formation on the extremities, mouth and snout and causes abortions and stillbirths if infection occurs during pregnancy. vesv is considered an exotic agent in the us, following its eradication in 1956. the single capsid protein gene of vesv serotype a48 was cloned and sequenced. the capsid amino acid sequence was 6 ...19989660070
organization of the canine calicivirus genome from the rna polymerase gene to the poly(a) tail.in recent years a wealth of data has become available about the caliciviruses that infect humans, as well as those which infect a range of animal species, notably cats, rabbits, pigs and marine animals. however, in the two decades since the earliest reports of calicivirus infection in dogs, very little has become known about the epidemiology, pathogenicity and molecular biology of the caliciviruses that may infect canines. in 1990, a canine calicivirus (cacv) was isolated from a 2-month-old diar ...199910211962
genetic analysis of the rna polymerase gene of caliciviruses from dogs and cats.caliciviruses that infect animals including humans cause a specific disease syndrome in their respective hosts. feline calicivirus (fcv) is a major pathogen of respiratory disease of cats, and human caliciviru is a causative agent of diarrhea. it has been suggested, furthermore, that fcv and newly recognized canine calicivirus (cacv) may also be possible causes of diarrhea in these animal species. in this study nucleotide sequence of the rna polymerase gene of two caliciviruses of canine origin, ...199910423680
development of a reverse transcription polymerase chain reaction procedure for the detection of marine caliciviruses with potential application for nucleotide sequencing.a reverse transcription polymerase chain reaction (rt-pcr) procedure is described for the detection of marine caliciviruses including vesicular exanthema of swine virus (vesv), san miguel sea lion virus (smsv), bovine tillamook virus (bcv bos-1) and caliciviruses (cv) isolated from dolphin (cetacean cv), gorilla (primate cv) and rattlesnake (reptile cv) using primers (1f and 1r) designed from the capsid-coding region of the viral genome. these primers were compared with those described by neill, ...199910507417
epizootic vesicular disease in captive california sea lions.an epizootic of vesicular disease occurred in a group of semi-domesticated california sea lions (zalophus californianus) during the months of april and may 1997. ten castrated mature male sea lions, ages 12 to 19 yr, were housed in three adjacent open-ocean net enclosures in san diego bay (california, usa). four animals (40%) developed oral and extremity vesicles, anorexia, and were reluctant to perform learned behaviors. one animal developed vesicles but maintained a normal appetite and behavio ...200010941736
viral and bacterial serology of free-ranging pacific walrus.serum or heparinized plasma samples were obtained between 1994 and 1996 from 20 male and 20 female adult free-ranging pacific walrus (odobenus rosmarus divergens) from st. lawrence island and round island, alaska. samples were screened for antibodies to some potentially pathogenic bacteria and viruses. no sample had detectable antibody to brucella spp. three of 40 (8%) had low antibody titers to leptospira interrogans serovars. phocine distemper virus antibodies were not detected. serologic resp ...200211838234
applied technique for increasing calicivirus detection in shellfish extracts.optimal detection of enteric rna viruses in clinical, environmental, and food products using reverse transcription-pcr (rt-pcr) when inhibitory substances in extracted sample materials are present.200212147071
crystallization and preliminary crystallographic analysis of san miguel sea lion virus: an animal calicivirus.the caliciviridae is a family of nonenveloped, icosahedral, positive-sense single-stranded rna viruses. this family of viruses consists of both animal and human pathogens. adapting human caliciviruses to cell culture has not been successful, whereas some animal caliciviruses, including san miguel sea lion virus, have been successfully propagated in vitro. here we report the crystallization of san miguel sea lion virus serotype 4 (smsv4) and the preliminary x-ray crystallographic analysis of the ...200312615540
inter- and intragenus structural variations in caliciviruses and their functional implications.the family caliciviridae is divided into four genera and consists of single-stranded rna viruses with hosts ranging from humans to a wide variety of animals. human caliciviruses are the major cause of outbreaks of acute nonbacterial gastroenteritis, whereas animal caliciviruses cause various host-dependent illnesses with a documented potential for zoonoses. to investigate inter- and intragenus structural variations and to provide a better understanding of the structural basis of host specificity ...200415163740
prevalence of vesivirus in a laboratory-based set of serum samples obtained from dairy and beef cattle.to examine sera obtained from dairy and beef cattle to detect antibodies against vesivirus and compare seroprevalence among cattle within the sample population.200616426220
prevalence and distribution of four serotypes of smsv serum neutralizing antibodies in wild animal populations.serum neutralizing antibodies to four serotypes of san miguel sea lion virus (smsv) were demonstrated in a variety of marine and terrestrial species. these results show a wide geographic distribution of sms viruses in the marine environment and indicate that certain terrestrial mammals have been infected with these so-called marine viruses. evidence is presented supporting the theory that unidentified submammalian marine species are a reservoir for smsv.197616498871
development of a real-time reverse transcription polymerase chain reaction assay for detection of marine caliciviruses (genus vesivirus).marine caliciviruses form a distinct lineage within the genus vesivirus (family caliciviridae). this group includes vesicular exanthema of swine virus (vesv) and san miguel sea lion virus (smsv) and other related viruses which have been proposed to be marine in origin isolated from a variety of terrestrial and marine animals. rapid and reliable detection of marine caliciviruses is important as these viruses appear to be widespread and can cause vesicular disease in a wide variety of susceptible ...200717187870
viral and bacterial serology of six free-ranging bearded seals erignathus barbatus.serum or heparinized plasma samples were obtained from 3 male (2 adult and 1 weaned calf) and 3 adult female free-ranging bearded seals erignathus barbatus in may of 1994, 1995, or 1996. blood samples were obtained from animals taken in subsistence hunts near st. lawrence island, alaska and screened for antibodies to a suite of bacteria and viruses potentially pathogenic for pinnipeds and/or humans. no samples had detectable antibodies to brucella spp., phocine distemper virus, influenza a virus ...200818828565
expression and self-assembly of virus-like particles from two genotypes of marine vesiviruses and development of an elisa for the detection of antibodies.sequences encoding the major and minor capsid proteins (vp1 and vp2) from two marine vesivirus isolates (steller sea lion viruses v810 and v1415) were engineered for expression of virus-like particles (vlps) in the baculovirus system. the resulting vlps were morphologically similar to native vesivirus virions. purified vlps were probed in immunoblots with pooled antisera specific for nine san miguel sea lion virus (smsv) types, and a predominant protein of approximately 60kda was detected. an en ...201019913368
characterization of san miguel sea lion virus populations using pyrosequencing-based methods.san miguel sea lion virus (smsv) is a small rna virus in the genus vesivirus with an unusually broad host range. three populations of smsv were examined by pcr amplification of the capsid precursor and putative helicase genes, followed by pyrosequencing. the populations were nasal swabs from two smsv infected california sea lions (zalophus californianus) from two different years, and a virus isolate from the earlier swab that was passaged in cell culture five times. in the capsid precursor, exte ...201019931646
cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of lagovirus.rabbit hemorrhagic disease was described in china in 1984 and can cause hemorrhagic necrosis of the liver within two or three days after infection. the etiological agent, rabbit hemorrhagic disease virus (rhdv), belongs to the lagovirus genus in the caliciviridae family. compared to other calicivirus, such as rnv and smsv, the structure of lagovirus members is not well characterized. in this report, structures of two types of wild rhdv particles, the intact virion and the core-like particle (clp ...201021203997
epitope insertion at the n-terminal molecular switch of the rabbit hemorrhagic disease virus t = 3 capsid protein leads to larger t = 4 capsids.viruses need only one or a few structural capsid proteins to build an infectious particle. this is possible through the extensive use of symmetry and the conformational polymorphism of the structural proteins. using virus-like particles (vlp) from rabbit hemorrhagic disease virus (rhdv) as a model, we addressed the basis of calicivirus capsid assembly and their application in vaccine design. the rhdv capsid is based on a t=3 lattice containing 180 identical subunits (vp1). we determined the stru ...201222491457
identification of human junctional adhesion molecule 1 as a functional receptor for the hom-1 calicivirus on human cells.the hom-1 vesivirus was reported in 1998 following the inadvertent transmission of the animal calicivirus san miguel sea lion virus to a human host in a laboratory. we characterized the hom-1 strain and investigated the mechanism by which human cells could be infected. an expression library of 3,559 human plasma membrane proteins was screened for reactivity with hom-1 virus-like particles, and a single interacting protein, human junctional adhesion molecule 1 (hjam1), was identified. transient e ...201728196955
the complete genome sequence of the san miguel sea lion virus-8 reveals that it is not a member of the vesicular exanthema of swine virus/san miguel sea lion virus species of the caliciviridae.the complete genome sequence of the san miguel sea lion virus-8 (smsv-8) was determined in this study. a comparison of this sequence to other calicivirus sequences in genbank showed that this virus is genetically distinct from the vesicular exanthema of swine virus/san miguel sea lion virus (vesv/smsv) strains and belongs to a novel clade within the vesivirus genus.201425502676
calicivirus antibodies in wild fox populations.three populations of wild foxes were sampled for serum neutralizing antibody to calicivirus (san miguel sea lion virus) types 1-5. neutralizing activity was detected in serum from gray foxes resident on santa cruz island, california, but not in arctic foxes from alaska. the results indicate that foxes may be naturally infected with caliciviruses, but their role in the transmission cycle is unknown.197724228972
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