| influence of the transduced 3'utr of the c-src oncogene on tumour growth induced by the v-src gene of avian sarcoma virus pr2257. | avian sarcoma virus pr2257 contains 952 bp transduced from the left part of the 3'utr of the chicken c-src oncogene. deletion mutants were constructed to determine the effect of the 3'utr on tumorigenicity in vivo and in vitro. in the presence of the 3'utr, tumours were 3.4 times larger in vivo, and tumorigenicity was increased 2.5-fold in vitro. several regulatory submotifs were also found within the 3'utr. parts of the 3'utr were cloned into the ltr cat plasmid and analysed for cat expression. ... | 1996 | 8683206 |
| transduction of the cellular src gene and 3' adjacent sequences in avian sarcoma virus pr2257. | when injected into chickens, a transformation-defective mutant of the prague c strain of rous sarcoma virus induced tumors at low incidence and after a long latency. one such tumor released a replication-defective virus designated pr2257. we molecularly cloned and sequenced the proviral dna from quail fibroblasts transformed by pr2257. comparison of pr2257 sequence with that of prague c, cellular src, and 3' adjacent cellular dna showed that the spliced version of the c-src gene and about 950 ba ... | 1989 | 2463376 |
| genome structure of a new avian sarcoma virus pr2257 determined by restriction analysis. | the genome structure of a new avian src-containing acute transforming virus, designated pr2257, was studied by restriction analysis. pr2257 was isolated from a sarcoma grown in a 155-day-old chick inoculated intraembryonally with a transformation-defective mutant of dapr-c retaining no src sequences. the 4.4-kb genome of pr2257 consists of the following regions: 5' ltr--spliced version of chicken c-src--incomplete env gene--3' ltr. this structure clearly indicates that pr2257 was generated by re ... | 1988 | 2854080 |