| endogenous new world primate retrovirus: interspecies antigenic determinants shared with the major structural protein of type-d rna viruses of old world monkeys. | a reverse transcriptase-containing virus has recently been isolated from a squirrel monkey (saimiri sciureus). molecular hybridization studies demonstrate that the squirrel monkey retrovirus (smrv) is endogenous to this new world primate, yet lacks detectable nucleotide sequence homology with cellular dnas of representative old world primates or with the genomes of previously isolated old world primate retroviruses. the 35,000-dalton major structural protein (p35) of smrv was purified and shown ... | 1977 | 74833 |
| primate retroviruses: immunological cross-reactivity between major structural proteins of new and old world primate virus isolates. | the major 35,000-molecular-weight internal antigen (p35) of the squirrel monkey retrovirus (smrv) was isolated and partially characterized. immunological analysis of smrv p35 led to the demonstration of antigenic determinants common to smrv and the mason-pfizer monkey virus (mpmv). a broadly reactive competition immunoassay was developed utilizing antiserum to mpmv to precipitate 125i-labeled smrv p35. although the major structural proteins of mpmv and smrv competed with equal efficiency in this ... | 1978 | 76686 |
| comparison of mason-pfizer monkey virus and squirrel monkey (saimiri sciureus) retrovirus by immunoelectron microscopy. | comparison of mason-pfizer monkey virus and squirrel monkey retrovirus (both candidate type d oncornaviruses) by immunoelectron microscopy revealed that these two viruses do not share common surface antigens. | 1978 | 79657 |
| natural distribution of squirrel monkey retrovirus proviral sequences in primate dnas. | 3h-labelled 70s rna of squirrel monkey retrovirus (smrv) hybridized to a high degree (greater than 52%) to the dna of various tissues of two squirrel monkeys. hybridization of the same probe to dnas of other primates including new world monkeys (woolly monkey, capuchin, owl monkey), old world monkeys (rhesus, african green), apes (gibbon, chimpanzee), and human (a204 cells infected with mpmv) revealed no significant hybridization. analysis of the kinetics of hybridization indicated that smrv pro ... | 1978 | 99487 |
| interspecies radioimmunoassay for the major structural proteins of primate type-d retroviruses. | a competition radioimmunoassay has been developed in which type-d retroviruses from three primate species compete. the assay utilizes the major structural protein (36,000 daltons) of the endogenous squirrel monkey retrovirus and antisera directed against the major structural protein (27,000 daltons) of the mason-pfizer monkey virus isolated from rhesus monkeys. purified preparations of both viruses grown in heterologous cells, as well as extracts of heterologous cells infected with squirrel monk ... | 1977 | 202962 |
| squirrel monkey retrovirus and herpesvirus saimiri: observation in the same cell following isolation. | electron microscopic examination of mink lung cells previously cultured with a squirrel monkey (saimiri sciureus) throat swab suspension revealed the presence of squirrel monkey retrovirus (smrv) and herpesvirus saimiri (hvs) coexisting within the same cells in culture. hvs was identified by serum neutralization, and the retrovirus isolate was identified as smrv by a morphologic examination, microimmunodiffusion analysis, and demonstration of an mg2+ preference for the rna-directed dna polymeras ... | 1979 | 225504 |
| molecular biology of type a endogenous retrovirus. | intracisternal a particles (iaps) are retrovirus-like structures consistently observable in a variety of mouse tumor cells such as myeloma and hybridoma and in early embryonic cells derived from rodents but nothing is known of their infectivity. mouse iaps contain a gag-like protein, a reverse transcriptase and a polyadenylated rna molecule (iap rna). dna sequences complementary to iap rna (iap genome) are interspersedly present in rodent such as mice, rats, chinese hamsters and syrian hamsters ... | 1990 | 1710682 |
| nucleotide sequence of the syrian hamster intracisternal a-particle gene: close evolutionary relationship of type a particle gene to types b and d oncovirus genes. | we determined the complete nucleotide sequence of the intracisternal a-particle gene, iap-h18, cloned from the normal syrian hamster liver dna. iap-h18 was 7,951 base pairs in length with two identical long terminal repeats of 376 base pairs at both ends. on the coding strand, imperfect open reading frames corresponding to gag and pol of the retrovirus genome were observed, whereas many stop codons were present in the region corresponding to env. the putative h18 gag gene (809 amino acids) had a ... | 1985 | 2991563 |
| immunological relationships of an endogenous guinea pig retrovirus with prototype mammalian type b and type d retroviruses. | a retrovirus endogenous to guinea pig cells was earlier shown to be morphologically similar to type b and type d prototype retroviruses. molecular hybridization techniques were used to show that guinea pig virus nucleotide sequences are endogenous to both domestic (cavia porcellus) and indigenous (cavia aperea) guinea pigs, but cannot be detected in the dna of either other hystricomorph rodents or other mammals tested. using radioimmunological techniques designed to detect interspecies relations ... | 1980 | 6154150 |
| biochemical studies of primate retroviruses. | in the present paper, recent biochemical studies of retroviruses carried out in our laboratory are summarized. protein compositions, peptide maps of internal structural proteins, neighborhoods of major structural proteins, and serological properties of reverse transcriptases of type d virus isolates from human cells (including graffi's isolate termed pmfv and also isolates from hela- and hep-2 cells) were compared with those of type d viruses from old world (mason-pfizer virus of rhesus monkeys, ... | 1983 | 6193765 |
| dna sequence relationship of the baboon endogenous virus genome to the genomes of other type c and type d retroviruses. | baboon endogenous virus (baev) is a type c retrovirus present in multiple proviral copies in the dna of baboons. although interspecies antigenic determinants present on reverse transcriptase and gag proteins are shared among all mammalian type c viruses, no nucleic acid homology between baev and other type c viruses (except rd-114) has been found in conventional liquid hybridization experiments. in this study, we used restriction fragments of cloned baev dna immobilized on nitrocellulose to test ... | 1982 | 6284972 |
| improved mg2+-based reverse transcriptase assay for detection of primate retroviruses. | the reverse transcriptase (rt) assay is a simple, relatively inexpensive, widely used assay that can detect all retroviruses (known and novel retroviruses as well as infectious and defective retroviruses) on the basis of the divalent cation requirement of their rt enzyme, i.e., mg2+ or mn2+. descriptions of various rt assays have been published; however, they cannot be directly applied to the analysis of biological products or clinical samples without further standardization to determine the low ... | 1999 | 10325311 |
| high throughput detection of retrovirus-associated reverse transcriptase using an improved fluorescent product enhanced reverse transcriptase assay and its comparison to conventional detection methods. | the development and application of a novel, sensitive taqman fluorescent probe-based product enhanced rt test (f-pert) for the detection of retrovirus are described. the assay allows discrimination between the amplification signals generated by genuine positive signals that result from retroviral rt activity and the rt-like activity from dna polymerases. the rt-like activity from dna polymerases was suppressed by the addition of activated calf-thymus dna with no reduction in the rt activity. a l ... | 1999 | 10894635 |
| adventitious virus detection in cells by high-throughput sequencing of newly synthesized rnas: unambiguous differentiation of cell infection from carryover of viral nucleic acids. | the use of high-throughput sequencing (hts) to identify viruses in biologicals differs from current molecular approaches, since its use enables an unbiased approach to detection without the need to design specific primers to preamplify target sequences. its broad range of detection and analytical sensitivity make it an important tool to ensure that biologicals are free from adventitious viruses. similar to other molecular methods, however, identification of viral sequences in cells by hts does n ... | 2019 | 31167947 |
| leukemic histiocytic sarcoma in a captive common squirrel monkey (saimiri sciureus) with saimiriine gammaherpesvirus 2 (rhadinovirus), saimiri sciureus lymphocryptovirus 2 (lymphocryptovirus) and squirrel monkey retrovirus (β-retrovirus) coinfection. | hematopoietic neoplasia other than lymphoma and leukemia is uncommon among non-human primates. herein, we provide the first evidence of occurrence of leukemic histiocytic sarcoma in a captive common squirrel monkey with saimiriine gammaherpesvirus 2 (rhadinovirus), saimiri sciureus lymphocryptovirus 2 (lymphocryptovirus), and squirrel monkey retrovirus (β-retrovirus) coinfection. | 2020 | 32412106 |