| search for specificity in natural cell-mediated cytotoxicity. | differences in antigenicity between the human osteosarcoma cell line te 85/b and its feline sarcoma virus-infected subline nih e1041 were detected by competitive inhibition of natural cell-mediated cytotoxicity (ncmc). whether the differences could be attributed to the viral infection was investigated by absorption and elution studies of antibodies that determine the specificity of ncmc against the cell lines. antibodies from the serum of healthy individuals were first absorbed onto target cells ... | 1979 | 92584 |
| apparent allosterism by avian myeloblastosis virus reverse transcriptase and e. coli dna polymerase i. | a recent report (1) presented evidence for allosterism in reverse transcription by mason-pfizer monkey virus reverse transcriptase and by e. coli dna polymerase i. our experiments also demonstrate these apparent cooperative effects when synthesis is catalyzed by either avian myeloblastosis virus dna polymerase, feline sarcoma virus dna polymerase, or e. coli dna polymerase i (large fragment). we show that the apparent cooperativity depends on the use of oligo(dt)12-18 as primer. however, if the ... | 1979 | 86985 |
| feline sarcoma virus in vitro infection of human cells. influence of chemical carcinogens on focus formation. | human fibroblast cells treated with benzo[alpha]pyrene (bp), aflatoxin b1 (afb1) or n-acetoxy-2-fluorenylacetamide (a-aaf) inhibited snyder-theilen feline sarcoma virus (st-fesv) focus formation. inhibition of focus formation resulting from chemical treatment was not related to cytotoxic concentrations of chemicals in that little or not effect on cells surviving treatment was observed. maximum inhibition of focus formation occurred with bp when the cells were treated before infection. by contras ... | 1978 | 81113 |
| immune response to leukemia virus and tumor-associated antigens in cats. | cats represent an unusually valuable model for studying the role of the immune response to leukemia, lymphoma, and other mesodermal neoplasms. the agents that cause spontaneous feline leukemias, lymphomas, and fibrosarcomas, the feline leukemia and sarcoma viruses, are well characterized. a specific tumor cell membrane antigen, designated the feline oncornavirus-associated cell membrane antigen (focma) has also been described. feline leukemia and feline sarcoma viruses are antigenically indistin ... | 1976 | 56224 |
| determination of subgroup-specific feline oncornavirus-neutralizing antibody. | a microneutralization assay was developed for antibody-to-subgroup-specific feline oncornaviruses. this study combines the economic advantage of a microtiter system and the quantitative focus reduction method which permits contruction of multiplicity curves for determination of virus-neutralizing titers. a twofold increase in synder-theilen feline sarcoma virus (st-fesv) on feline embryo cells decreased by approximately twofold the titer of reference goat serum prepared against kawakami-theilen ... | 1975 | 173656 |
| immunological studies of tumors induced in sheep by injecting ovine cells transformed in vitro with feline sarcoma virus. | | 1975 | 183703 |
| defectiveness of a feline sarcoma virus demonstrable by the acquisition of a new envelope antigenic type by phenotypic mixing. | | 1977 | 193272 |
| [model studies on virus-induced tumors and their immunological treatment (author's transl)]. | after a review of the general biological properties of c-type oncorna viruses, results are presented on the structure of an exogenous murine leukemia virus (flv) and on the serobiological properties of its structural proteins. our findings suggested a major role of the viral surface glycoprotein gp71 in immunological defense mechanisms. this was confirmed by vaccination experiments with isolated gp71 in mice. the induced immunity was highly specific and not operative against endogenous murine c- ... | 1977 | 198602 |
| biological properties and translational products of three independent isolates of feline sarcoma virus. | | 1979 | 217170 |
| feline sarcoma virus-coded polyprotein: enzymatic cleavage by a type c virus-coded structural protein. | a purified 15,000-molecular-weight (mr) prague strain rous sarcoma virus gag gene-coded structural protein, p15, was shown to enzymatically cleave the previously described 130,000 mr feline sarcoma virus-coded polyprotein, pr130. cleavage products included proteins ranging in molecular weight from 12,000 to 110,000. the specificity of this cleavage reactivity was indicated by the fact that, under similar conditions, neither purified type c viral structural proteins nor nonviral proteins such as ... | 1979 | 219252 |
| involvement of different exogenous feline leukemia virus subgroups in the generation of independent feline sarcoma virus isolates. | | 1979 | 224571 |
| susceptibility of human cell lines to feline leukemia virus and feline sarcoma virus. | the susceptibility of human fibroblasts and human lymphoid cell lines to feline leukemia virus (felv) and feline sarcoma virus (fesv) was investigated. human cells were highly sensitive to infection by felv subgroups b and c and fesv but resistant to infection by felv subgroup a. the cells became infected, produced infectious virus, and displayed no differences in morphology or viability. t-cell lines appeared to be more sensitive to felv and fesv infection and to produce more virus than did aut ... | 1979 | 228103 |
| nature and distribution of feline sarcoma virus nucleotide sequences. | the genomes of three independent isolates of feline sarcoma virus (fesv) were compared by molecular hybridization techniques. using complementary dnas prepared from two strains, sm- and st-fesv, common complementary dna's were selected by sequential hybridization to fesv and feline leukemia virus rnas. these dnas were shown to be highly related among the three independent sarcoma virus isolates. fesv-specific complementary dnas were prepared by selection for hybridization by the homologous fesv ... | 1979 | 225544 |
| role of regional and distant lymph nodes in rejection of feline sarcoma virus-induced tumors in sheep. | the immune response of regional and distant lymph nodes was compared relative to rejection of feline sarcoma virus (fesv)-induced tumors in sheep. following injection of fesv-transformed allogeneic or autochthonous fibroblasts into the lower leg, small tumors developed at the site of inoculation and subsequently regressed. efferent lymph from the regional popliteal nodes and distant nodes in the same host was collected for periods up to 40 days after tumor cell inoculation. the cell response in ... | 1979 | 222930 |
| monkey interferon: activity on human cells and chromatographic properties. brief report. | monkey interferon (mkif) produced in monkey bsc-1 cells infected with newcastle disease virus showed antiviral activity on human foreskin fibroblasts and rd114cells--a human line transformed by feline sarcoma virus. the titer of the monkey interferon in human cells was 10--30 fold greater than that found in several normal monkey (bsc-1, cv-1 or sv 40 transformed (c2, c6, t-22) monkey cell lines tested. ten to fifteen-fold purification of mkif without loss of activity could be achieved by chromat ... | 1979 | 222238 |
| immunosuppressive properties of a virion polypeptide, a 15,000-dalton protein, from feline leukemia virus. | the 15,000-molecular-weight polypeptide (p15) of feline leukemia virus (felv) was shown to impair normal lymphocyte function in vitro and to abrogate immunity to feline oncornavirus disease in vivo. felvp15 suppressed concanavalin a-induced blast transformation of normal feline lymphocytes by 68%, while other virion proteins had no effect. p15 suppression was not due to toxicity, nor was p15 a competitive inhibitor of concanavalin a binding. capping of receptors for concanavalin a on normal feli ... | 1979 | 218725 |
| characterization of a feline sarcoma virus-coded antigen (focma-s) by radioimmunoassay. | a radioimmunoassay has been developed that detects a unique antigen encoded by the genome of the feline sarcoma virus (fesv). pseudotype viral particles containing an fesv-specific polyprotein (p85) were used both as a source of antigen and to prepare specific antisera in rabbits. because p85 contains antigens related to two structural proteins (p15 and p12) of feline leukemia virus (felv), antibodies directed to these were adsorbed with purified felv proteins. the adsorbed rabbit antiserum boun ... | 1978 | 212755 |
| pseudotypes of feline sarcoma virus contain an 85,000-dalton protein with feline oncornavirus-associated cell membrane antigen (focma) activity. | feline sarcoma virus (fesv) rescued from transformed nonproducer mink or rat cells contains two fesv-specific antigens (p15 and p12), and the feline oncornavirus-associated cell membrane antigen (focma). all three antigens are helper virus-independent and are encoded by the fesv genome, focma, p15, and p12 antigens cochromatograph as phosphorylated molecules of 85,000 molecular weight (pp85), adsorb to immunoadsorbant columns prepared with antibodies to feline leukemia virus (felv), and are prec ... | 1978 | 206902 |
| relationship between feline leukemia virus antigen expression and viral infectivity in blood, bone marrow, and saliva of cats. | correlation was greater than 90% between feline leukemia virus (felv), group-specific antigen (gsa) in leukocytes, and viral infectivity (vi) in serum or plasma from 132 cats infected with either the rickard strain of felv, the snyder-theilen strain of feline sarcoma virus, or field strains of felv. detection of gsa in blood cells was at least as sensitive as detection of vi in serum. in 45% of felv gsa-positive cats inoculated with felv-rickard strain, vi was detected in saliva. no saliva sampl ... | 1977 | 198120 |
| feline oncornavirus-associated cell membrane antigen: evidence for an immunologically crossreactive feline sarcoma virus-coded protein. | the feline oncornavirus-associated cell membrane antigen (focma) acts as a target for natural immuno-surveillance against tumor development in the cat. in the present study, mink and rat cells nonproductively transformed by feline sarcoma virus (fesv) were shown to express focma as well as 5'-terminal feline leukemia virus (felv) gag gene proteins, p15 and p12. in contrast, such cells lack detectable levels of other felv gag gene-coded proteins or the env gene product, gp70. focma, p15, and p12 ... | 1977 | 202959 |
| tyrosine protein kinase activity of the hz4-feline sarcoma virus p80gag-kit-transforming protein. | the hardy-zuckerman 4 feline sarcoma virus (hz4-fesv), isolated from a feline fibrosarcoma, is a replication defective acute transforming feline retrovirus that originated by transduction of feline c-kit sequences with feline leukemia virus (felv). the v-kit sequences of the hz4-fesv, a segment of 1106 nucleotides, correspond to sequences of the cytoplasmic domain of the c-kit receptor kinase. the hz4-fesv is known to encode an 80-kilodalton protein with felv gag and kit determinants. the p80gag ... | 1990 | 1697669 |
| detection and evaluation of feline oncornavirus-induced cell surface antigen(s) shed from cells in vitro. | a method for preparation of soluble feline oncornavirus-induced cell surface antigens was described. this technique relied upon the natural release of antigen(s) from fl-74 feline lymphoblastoid cells during their maintenance at 37 degrees in serum-deficient medium. when concentrated and clarified spent medium from 4-day cultures was tested for its antigen content by inhibition of humoral cytotoxicity, it was found that this natural production of soluble antigen provided more feline oncornaviru ... | 1977 | 198129 |
| feline leukemia virus: biochemical and immunological characterization of gag gene-coded structural proteins. | the major non-glycosylated structural proteins of feline leukemia virus have been isolated, and competition immunoassays have been developed for each. these proteins include the 27,000- to 30,000-molecular-weight major internal antigen designated p30, a 15,000-molecular-weight protein (p15), an acidic protein of 12,000 molecular weight (p12), and a highly basic 10,000-molecular-weight protein (p10). immunologically and biochemically corresponding proteins of feline and murine leukemia viruses ha ... | 1977 | 197262 |
| feline oncornavirus-associated cell membrane antigen: expression in transformed nonproducer mink cells. | the feline oncornavirus-associated cell membrane antigen (focma) is a target for naturally occurring immunity that protects the cat against development of fibrosarcoma and leukemia. feline sarcoma virus-transformed "nonproducer" mink cells express high levels of focma, but not the major viral structural proteins. transformation of the same cells by murine sarcoma virus, or infection with feline leukemia virus, which is nontransforming for epithelial or fibroblastic cells, did not induce focma. t ... | 1977 | 194310 |
| susceptibility of xeroderma pigmentosum cells to transformation by murine and feline sarcoma viruses. | among various strains of skin fibroblasts tested, two strains derived from xeroderma pigmentosum (xp) patients (ages 19 and 25) with neurological complications and two strains obtained from heterozygotes (ages 54 and 18) showed relatively higher susceptibility than normal age-matched controls to transformation by feline sarcoma virus (fsv). only one strain from a normal individual also showed a high susceptibility. generally, there was a parallelism in susceptibilities to fsv and kirsten murine ... | 1976 | 184947 |
| a biochemical and genetic analysis of mammalian rna-containing sarcoma viruses. | current studies have shown that all mammalian sarcoma-producing viruses, whether isolated from laboratory experiments of naturally occurring tumors, are deletion mutants of replicating mammalian type c viruses. nonproducer cells transformed by any of these sarcoma viruses contain rna homologous to mammalian leukemia viruses, even though the cells contain no known proteins currently coded for by the mammalian leukemia virus. this mammalian leukemia virus information (ft-) is a genetically stable ... | 1975 | 169043 |
| immunization against feline oncarnavirus disease using a killed tumor cell vaccine. | specific pathogen-free cats were immunized with an inactivated feline oncornavirus tumor cell vaccine. immunized cats produced high antibody titers to the feline oncornavirus-associated cell membrane antigen and were protected from oncogenic feline sarcoma virus challenge. however, immunization did not produce virus-neutralizing antibody nor did it prevent viremia. | 1976 | 182360 |
| regression of feline sarcoma virus-induced sarcomas in dogs. ii. immunologic investigations. | the serial development of cell-mediated immunity (cmi), cytotoxic antibody activity, serum blocking activity, and virus-neutralizing antibody levels were monitored in vitro for beagle and mongrel puppies inoculated with feline sarcoma virus (fesv) and were compared to in vivo histologic markers of regression of induced sarcomas. cmi developed rapidly and maintained a high level of in vitro activity throughout the tumor life-span. cytotoxic antibody levels similarly rose rapidly to peak just befo ... | 1975 | 46276 |
| abrogation of resistance to feline oncornavirus disease by immunization with killed feline leukemia virus. | four-week-old specific-pathogen-free cats were immunized with a combined vaccine composed of killed feline leukemia virus and killed feline oncornavirus-associated cell membrane antigen-containing tumor cells. immunization induced feline oncornavirus-associated cell membrane antigen antibody titers ranging from 1:32 to 1:256 but did not elicit detectable virus-neutralizing antibody titers. kittens immunized with tumor cells alone developed higher feline oncornavirus-associated cell membrane anti ... | 1977 | 67886 |
| quantitative nucleotide sequence relationships of mammalian rna tumor viruses. | a molecular hybridization technique has been used to quantitatively measure the nucleotide sequence relationships of selected mammalian rna tumor viruses. reciprocal cross-hybridization tests were done in which a given radioactively labeled, viral genomic rna species was annealed with an excess of unlabeled, complementary dna product synthesized in endogenously instructed reverse transcriptase reactions. hybrid formation was measured with pancreatic rnase a. three representative mammalian rna tu ... | 1975 | 49442 |
| transformation of horse skin cells by type-c sarcoma viruses. | a horse skin cell line (e. derm, nbl-6, ccl-57) was susceptible to focus formation by the kirsten mouse sarcoma virus, feline sarcoma virus (st stain) and the msv pseudotypes with woolly monkey, gibbon monkey, rd-114, at-124, baboon placenta and murine xenotropic (balb/c 3t3 and c57l/jd) type-c viruses. foci were detected within 5 days after infection and the transformed cells continued to produce infectious virus and group-specific antigen of their respective type-c leukemia viruses. the transf ... | 1975 | 165152 |
| the association of viruses with urveal melanoma. | electron microscopic examination of 57 ocular melanomas (54 human, two feline and one canine) revealed the presence of viral particles in six specimens. herpesviruses particles were observed in one human specimen and were passed in human fibroblasts (wi-38), where they gave rise to intranuclear inclusions. a-type oncornavirus particles (oncogenic rna virus) were observed in a second case, both in cells of tumor directly removed from an enucleated eye as well as in cells grown in tissue culture. ... | 1979 | 545833 |
| regression of feline sarcoma virus-induced sarcomas in dogs. i. morphologic investigations. | in a study of morphologic changes in the development and regression of feline sarcoma virus (fesv)-induced tumors in dogs, 27 weaned and newborn beagle and mongrel puppies were inoculated with fesv in doses from 1.0 to 3.0 geq; 2 beagle and 2 mongrel puppies were used as uninoculated contact controls. all animals were examined daily, and crude tumor volume was calculated from length, width, and depth measurements of the neoplasms. biopsies were done at various stages of tumor development and reg ... | 1975 | 1113321 |
| experimental oncornavirus vaccines in the cat. | an experimental approach to the immunoprophylatic control of feline oncornavirus-mediated diseases has included induction of antivirus immunity and antibodies to the feline oncornavirus-associated membrane (tumor) antigens. a suitable model for exploring the effectiveness of killed oncornavirus vaccines in the cat has been provided by the use of feline sarcoma virus. immunization of seven pregnant queens over a 6-week period with ultraviolet light-inactivated gardner-arnstein feline sarcoma viru ... | 1976 | 943234 |
| feline oncornavirus-associated cell membrane antigen. v. humoral immune response to virus and cell membrane antigens in cats inoculated with gardner-arnstein feline sarcoma virus. | tumor growth responses in 5- to 6-week-old kittens inoculated with the gardner-arnstein strain of feline sarcoma virus exhibited three distinct pattern: 1) complete tumor regression or no detectable tumor growth in approximately one-third of 43 inoculated kittens, 2) rapid tumor progression which led to debilitation and death within 16.2 +/- 4.2 weeks following infection in an additional one-third, and 3) slow tumor growth or temporary regressions in the remaining third. the feline oncornavirus- ... | 1975 | 1206756 |
| leukemia specific antigens: focma and immune surveillance. | in cats, horizontally transmitted viruses cause leukemia and lymphoma under natural conditions. as with other retroviruses, feline leukemia virus (felv) contains products of 3 major genes; the virus core gag gene products, the polymerase, and the virus envelope glycoprotein. when cells are transformed in vitro by the related feline sarcoma virus (fesv), an additional protein, focma is expressed at the cell membrane. focma, which is fesv-coded, is transformation and/or tumor specific and expresse ... | 1979 | 232469 |
| restriction endonuclease mapping of unintegrated proviral dna of snyder-theilen feline sarcoma virus: localization of sarcoma-specific sequences. | extrachromosomal dna purified from mink cells acutely infected with the snyder-theilen strain of feline sarcoma virus (fesv) was digested with restriction endonucleases, and the dna fragments were electrophoretically separated, transferred to a solid substrate, and hybridized with radiolabeled dna transcripts complementary to different portions of the fesv rna genome. major dna species 8.4 and 5.0 kilobase pairs (kbp) long represent the linear, unintegrated proviruses of snyder-theilen feline le ... | 1979 | 229270 |
| a statistical evaluation of the binding of ferritin-conjugated lectins to the surface of rat cells. topographical variations as a function of lectin concentration and cell type. | statistical procedures were utilized to evaluate the concentration dependence of labeling by ferritin-conjugated lectins on four different rat cells: hepatocytes, normal thymocytes, friend virus-induced rat tumor cells and feline sarcoma virus-induced rat sarcoma cells. labeling by ferritin conjugates of concanavalin a, wheat germ agglutinin and ricinus communis agglutinins i and ii was quantitated by counting the number of ferritin granules on 600 angstrom membrane segments. relationships betwe ... | 1979 | 316439 |
| influence of glucocorticoid, estrogen, and androgen hormones on transformation of human cells in vitro by feline sarcoma virus. | infection of human foreskin cells (d-550) by the snyder-theilen strain of feline sarcoma virus produced small but countable foci and demonstrated "single-hit" dose-response kinetics. significant quantitative and qualitative enhancement of focus formation was observed when the glucocorticoid hormones, dexamethasone, hydrocortisone, cortisol acetate, and prednisolone were added to cell cultures (1.0 mug/ml) 24 hr postinfection. however, aldosterone, while inducing qualitatively larger foci, did no ... | 1976 | 1268852 |
| conversion of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus (st:fesv): tumoricidal potential in monolayers and in agar suspensions. | in earlier studies [1-3], we have demonstrated the conversion of human fibroblasts (hf) to tissue macrophages (tm) by the snyder-theilen feline sarcoma virus (st:(fesv)). the purpose of the present study is to determine the cytolytic potential of st:fesv(felv)-induced tm against tumorigenic target cells under defined conditions in vitro. the results show that st:fesv-induced tm, but not mock-infected hf, produced significant lysis of human colon adenocarcinoma cells (ls-180) after a 3-day preinc ... | 1992 | 1316189 |
| suppression of transformed phenotype in hybrids of v-fgr and v-raf transformed rat-1 cells with rat embryonic fibroblasts is due to transcriptional inactivation of viral oncogenes. | rat-1 cells that had been transformed to tumorigenicity by transfection with the retroviral oncogenes v-raf from 3611-murine sarcoma virus, or v-fgr from gardner-rasheed feline sarcoma virus were fused with rat embryonic fibroblasts at an early passage. in both fusion experiments hybrid cells were isolated that exhibited normal morphology, anchorage requirement for proliferation, and either no tumorigenicity (v-fgr) or extended latency periods for tumor growth (v-raf) in nude mice. transcription ... | 1992 | 1563491 |
| replication of feline sarcoma virus in cat embryonic kidney cells in the absence of transformation. | the infection of cat embryonic kidney cells (crc) with gardner and snyder-theilen strains of feline sarcoma viruses (ga-fesv and st-fesv) do not lead to transformation and focus formation. the focus forming insusceptibility is shown to be stable and nontransmissable property of these cells. however, the crc cells are permissive for st-fesv, and the viral production is 10 to 100 folds lower than in focus forming susceptible cat cells. produced st-fesv(c) by crc cells induced on cat embryonic cell ... | 1975 | 1214923 |
| retrovirus transformation associated secretory phosphoproteins of mouse and mink cells. | using antisera to major excreted protein (mep) of kirsten sarcoma virus transformed nih 3t3 (knih) cells, we have identified phosphoproteins from the media of feline sarcoma virus (fesv) transformed mink cells. these secretory phosphoproteins from fesv-transformed mink cells are of 35 kda m.w. and they do not have autophosphorylation activity. a comparative analysis of mep from the media of transformed mouse and mink cells was performed on the basis of proteolytic cleavage products and acid hydr ... | 1992 | 1559243 |
| conversion of human fibroblasts to tissue macrophages by the snyder-theilen feline sarcoma virus (st:fesv) is associated with the de-novo expression of il-1 alpha, il-1 beta, ifn-alpha, tnf-alpha, gm-csf, and cd4. | in an earlier study, we have demonstrated the conversion of human fibroblasts (hf) to tissue macrophages (tm) by the snyder-theilen feline sarcoma virus (st:(fesv)) [1]. the present study shows that conversion of cultured hf by the st:fesv to tm resulted in the de-novo expression of interleukin-1 alpha, il-1 beta, interferon-alpha, tumor necrosis factor-alpha, granulocyte-macrophage colony stimulating factor, and cd4. the conversion of hf to tm was also associated with increased expression of no ... | 1991 | 1667481 |
| development and characterization of a panel of monoclonal antibodies against the catalytic domain of the human fes proto-oncogene product. | in developing monoclonal antibodies (moabs) against the human fes proto-oncogene product, recombinant dna technology was used to target reactivity of the moabs towards the catalytic domain of it. therefore, sequences of human fes exons 15-19 encoding amino acid residues 612 to 822 which harbor the catalytic domain except the presumed atp-binding region, were fused in phase to the bacterial trp e gene which encodes anthranilate synthase. after partial purification of it, the bacterially produced ... | 1992 | 1545781 |
| a dominant suppressive mutation in a cellular gene restores the nontransformed phenotype to v-fms-transformed mink cells. | a nontransformed revertant subclone (gvr17) was isolated from mutagen-treated populations of mink epithelial cells which had been transformed by the susan mcdonough strain of feline sarcoma virus (sm-fesv). the revertant cells, although contact-inhibited for growth and unable to form colonies in semisolid medium, contained two copies of integrated sm-fesv proviral dna, expressed high levels of the v-fms oncogene product and its associated tyrosine kinase activity, and yielded rescuable viruses a ... | 1991 | 2052353 |
| feline immunodeficiency virus and feline leukemia virus infections and their relationships to lymphoid malignancies in cats: a retrospective study (1968-1988). | sera from 353 cats with naturally occurring feline leukemia virus (felv) infection were collected in boston, los angeles, new york city, and seattle between 1968 and 1988. these sera were retrospectively assayed by enzyme-linked immunosorbent assay for antibodies to feline immunodeficiency virus (fiv). fifty-one (14.4%) of the felv-positive sera had antibodies to fiv, indicating dual oncovirus and lentivirus infections. fiv infections were confirmed by western blot analysis, antibodies against t ... | 1990 | 2159993 |
| transforming mechanism of the feline sarcoma virus encoded v-fms oncogene product. | the v-fms oncogene product encoded by the mcdonough strain of feline sarcoma virus (sm-fesv) is a transmembrane glycoprotein which belongs to the tyrosine kinase receptor family. the cellular counterpart, the c-fms product, is the receptor for macrophage colony stimulating factor (m-csf or csf-1). the v-fms and the c-fms product differ structurally only in seven point mutations and in their c-terminal domains. we have corrected the published sequence of the v-fms product and found that the new c ... | 1991 | 1834054 |
| histiocytic conversion of human adult skin fibroblasts by the snyder-theilen feline sarcoma virus. | oncogenes are apparently involved in the transformation/neodifferentiation of human cells. we now report a novel example of transformation/neodifferentiation: the specific conversion of cultured human adult skin fibroblasts (hsf) to histiocytes (tissue macrophages (tm)) by the snyder-theilen feline sarcoma virus (st: (fesv)). the de novo conversion of cultured hsf was demonstrated in a large fraction of st:fesv (felv)-transformed foci in the presence of dexamethasone (dx). identification of tiss ... | 1990 | 1966584 |
| reassessment of the v-fms sequence: threonine phosphorylation of the cooh-terminal domain. | the v-fms oncogene product of the mcdonough strain of feline sarcoma virus is a member of the receptor tyrosine kinase family. its cellular counterpart, the c-fms product, is the receptor for colony-stimulating factor 1 (csf-1) of macrophages. we have reanalyzed the v-fms gene by direct sequencing of a biologically active clone. an additional a nucleotide was detected in position 2810 of the published v-fms sequence. the frameshift changed the cooh-terminal sequence of the v-fms protein from -r- ... | 1991 | 1833563 |
| molecular cloning of the feline c-fes proto-oncogene and construction of a chimeric transforming gene. | the feline c-fes proto-oncogene, different parts of which were captured in feline leukemia virus (felv) to generate the transforming genes (v-fes) of the gardner-arnstein (ga) strain of feline sarcoma virus (fesv) and the snyder-theilen strain (st) of fesv, was cloned and its genetic organization determined. southern blot analysis revealed that the c-fes genetic sequences were distributed discontinuously and colinearly with the v-fes transforming gene over a dna region of around 12.0 kb. using c ... | 1985 | 2993104 |
| activation of murine c-abl protooncogene: effect of a point mutation on oncogenic activation. | activation of the c-abl protooncogene occurs in abelson murine leukemia virus, in hardy-zuckerman 2 feline sarcoma virus, and during the chromosomal translocations that generate bcr-abl gene fusion products. to study the molecular mechanism involved in the c-abl activation, we have created a series of modifications in murine c-abl and assayed these constructs for oncogenic activity using the nih 3t3 cell transformation assay. our results show that amino-terminal deletions are sufficient for onco ... | 1990 | 2168550 |
| transformation of chicken fibroblasts by the v-fms oncogene. | the v-fms oncogene of the mcdonough strain of feline sarcoma virus (sm-fesv) encodes a plasma-membrane-associated tyrosine kinase (gp140v-fms) which is closely related, both structurally and functionally, to the c-fms-specified receptor for the macrophage colony stimulating factor (csf-1). in mammalian fibroblasts, the natural producers of csf-1, expression of v-fms leads to cell transformation. to study the interaction between csf-1 and gp140v-fms molecules in a cell system that does not produc ... | 1990 | 2171188 |
| structural alteration of viral homologue of receptor proto-oncogene fms at carboxyl terminus. | a role for proto-oncogenes in the regulation and modulation of cell proliferation has been suggested by the findings that the b-chain of platelet-derived growth factor (pdgf) is encoded by the proto-oncogene sis and that the erb-b oncogene product is a truncated form of the epidermal growth factor (egf) receptor. furthermore, the product of the proto-oncogene fms (c-fms) may be related or identical to the receptor for macrophage colony-stimulating factor (csf-1). v-fms is the transforming gene o ... | 1986 | 2421165 |
| expression of the c-fms proto-oncogene during human monocytic differentiation. | the mcdonough strain of the feline sarcoma virus contains a transforming gene (v-fms) which contains partial nucleotide homology with proto-oncogenes encoding tyrosine kinases. one of the v-fms-encoded products, gp140fms, is a cell surface transmembrane glycoprotein that may function as a growth factor receptor. although c-fms transcripts have been detected in placental trophoblasts and normal human bone marrow, the role of the c-fms gene product is unknown. we now report that induction of monoc ... | 1985 | 2409450 |
| gp140v-fms molecules expressed at the surface of cells transformed by the mcdonough strain of feline sarcoma virus are phosphorylated in tyrosine and serine. | cells transformed by the mcdonough strain of feline sarcoma virus express at their surface a v-fms-specific transmembrane glycoprotein designated gp140v-fms. by labeling with 32pi, gp140v-fms was shown to be phosphorylated 30-fold more in serine residues than were the cytosolic v-fms polypeptides gp180gag-fms and gp120v-fms. by using the phosphotyrosine phosphatase-specific inhibitor sodium orthovanadate, an additional tyrosine phosphorylation was observed in vivo, again involving predominantly ... | 1986 | 2432405 |
| fgr proto-oncogene mrna induced in b lymphocytes by epstein-barr virus infection. | several acute transforming retroviruses encode tyrosine-specific protein kinases which possess structural and functional relationships to cell-surface receptors for certain growth factors. one such tyrosine kinase is encoded by the onc gene, v-fgr, of gardner-rasheed feline sarcoma virus (gr-fesv). recently, we have isolated and characterized the human gene, c-fgr, corresponding to the viral onc sequence and have shown that c-fgr is a unique gene located on the short arm of chromosome 1 (ref. 7) ... | 1986 | 3003578 |
| human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. | structural features of v-kit, the oncogene of hz4 feline sarcoma virus, suggested that this gene arose by transduction and truncation of cellular sequences. complementary dna cloning of the human proto-oncogene coding for a receptor tyrosine kinase confirmed this possibility: c-kit encodes a transmembrane glycoprotein that is structurally related to the receptor for macrophage growth factor (csf-1) and the receptor for platelet-derived growth factor. the c-kit gene is widely expressed as a singl ... | 1987 | 2448137 |
| primary structure of c-kit: relationship with the csf-1/pdgf receptor kinase family--oncogenic activation of v-kit involves deletion of extracellular domain and c terminus. | the protein kinase domains of v-kit, the oncogene of the acute transforming feline retrovirus hz4-fesv (hz4-feline sarcoma virus), csf-1r (macrophage colony stimulating factor receptor) and pdgfr (platelet derived growth factor receptor) display extensive homology. because of the close structural relationship of v-kit, csf-1r and pdgfr we predicted that c-kit would encode a protein kinase transmembrane receptor (besmer et al., 1986a; yarden et al., 1986). we have now determined the primary struc ... | 1988 | 2456920 |
| the proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse w locus. | mice carrying mutations at the w locus located on chromosome 5 are characterized by severe macrocytic anaemia, lack of hair pigmentation and sterility. mutations at this locus appear to affect the proliferation and/or migration of cells during early embryogenesis and result in an intrinsic defect in the haematopoietic stem cell hierarchy. an understanding of the molecular basis of the complex and pleiotropic phenotype in w mutant mice would thus provide insights into the important developmental ... | 1988 | 2457811 |
| c-kit protein, a transmembrane kinase: identification in tissues and characterization. | the proto-oncogene c-kit encodes a transmembrane kinase which is related to the receptors for colony-stimulating factor type 1 and platelet-derived growth factor, as well as to the immunoglobulin superfamily. antibodies specific for the kinase domain of the p80 gag-kit protein of the hardy-zuckerman 4 feline sarcoma virus were prepared. these kit-specific antibodies were used to identify and characterize the c-kit protein in cat brain tissue. the c-kit protein product displays an autophosphoryla ... | 1988 | 2463468 |
| insulin-like growth factor i receptor gene is concordant with c-fes protooncogene and mouse chromosome 7 in somatic cell hybrids. | the insulin-like growth factor i (igf-1) mediates the actions of pituitary growth hormone in a variety of tissues. its receptor (igf1r) displays considerable structural similarity to the insulin receptor. in humans, the igf1r gene has been mapped near fes, the cellular counterpart of the feline sarcoma virus transforming gene v-fes, at the q25-q26 region of human chromosome 15 (hsa15). here, we report the mapping of mouse igf1r to mouse chromosome 7 (mmu7) by somatic cell hybrid analysis. this r ... | 1989 | 2548293 |
| relationship between c-src tyrosine kinase activity and the control of glucose transporter gene expression. | it has been shown previously that the rate of glucose transport in fibroblasts is accelerated by oncoproteins such as v-src, ras, and the transforming protein of feline sarcoma virus. this induction of glucose transport is associated with, and presumably caused by, induction of hep-g2/rat brain glucose transporter gene expression. to determine the mechanism underlying the induction of glucose transporter gene expression by the v-src oncogene we studied cell lines that overexpress the normal coun ... | 1989 | 2481817 |
| detection of fms-oncogene-specific tyrosine kinase activity in human leukemia cells. | the c-fms protooncogene encodes the receptor for the colony-stimulating factor 1 of macrophages. its transforming counterpart, the v-fms oncogene has previously been recognized as the transforming gene of the mcdonough strain of feline sarcoma virus. we have isolated rabbit antisera against a 115-kda recombinant polypeptide containing the 926 carboxy-terminal amino acids of the v-fms protein. all antibodies recognized the cytoplasmic domain of the v-fms protein, which is 95% homologous to the co ... | 1989 | 2526817 |
| the kinase activity of the v-fms encoded protein has a low ph optimum. | the protein encoded by v-fms, the oncogene of the susan mcdonough strain of feline sarcoma virus, is a member of the protein tyrosine kinase family. the kinase activity of the v-fms encoded protein has been reported to be low compared to other members of this enzyme family. we found that the optimal ph in vitro for the autophosphorylation of the immunoprecipitated v-fms encoded protein kinase activity was about ph 5.0; the activity at this ph was 15-fold higher than at the ph (7.4) used in stand ... | 1989 | 2654815 |
| scrambled duplications in the feline leukemia virus gag gene: a putative pattern for molecular evolution. | the present study is a detailed computer-assisted analysis of the feline leukemia virus gag gene nucleotide sequence together with its flanking sequences (st-felv gag) that is compared with the aligned sectors of the moloney strain of murine leukemia virus (mo-mulv gag) and of three strains of feline sarcoma virus. it shows that perfectly matched repeated oligomers up to 13 nucleotides long are overrepresented and scattered throughout both st-felv gag and mo-mulv gag, in noncoding and coding sec ... | 1989 | 2553988 |
| detection and cloning of new htlv-related endogenous sequences in man. | human t-cell leukemia virus (htlv) type i-related endogenous sequences (hres) have been cloned from a human genomic library. hres-1/1 is present in dna of all normal donors examined. by nucleotide sequence analysis, hres-1/1 contains two potential open reading frames capable of encoding a p25 and a p15. a 684 bp flanking region 5' from the first atg codon of p25 contains a tata-box, a poly-adenylation signal, a putative trna primer binding site, and inverted repeats at locations which are typica ... | 1989 | 2780312 |
| analysis of functional domains of the v-fms-encoded protein of susan mcdonough strain feline sarcoma virus by linker insertion mutagenesis. | the susan mcdonough strain of feline sarcoma virus contains an oncogene, v-fms, which is capable of transforming fibroblasts in vitro. the mature protein product of the v-fms gene (gp140fms) is found on the surface of transformed cells; this glycoprotein has external, transmembrane, and cytoplasmic domains. to assess the functional role of these domains in transformation, we constructed a series of nine linker insertion mutations throughout the v-fms gene by using a dodecameric bamhi linker. the ... | 1987 | 2823125 |
| transformation/neodifferentiation of human skin fibroblasts by acute oncornaviruses and dexamethasone. | we demonstrated that the kirsten murine sarcoma virus (kimsv) and the harvey murine sarcoma virus (hamsv) converted human skin fibroblasts (hsf) into adipocytes. adipocytic conversion of hsf by kimsv and hamsv was dependent on the presence of glucocorticosteroids. the kirsten murine leukemia virus, the harvey murine sarcoma [corrected] virus and the amphotropic helper virus (ap292) were ineffective by themselves. balb murine sarcoma virus and moloney murine sarcoma virus were, to a lesser degree ... | 1988 | 2852128 |
| structure and origins of the hz2-feline sarcoma virus. | the hz2-feline sarcoma virus (hz2-fesv) is a replication-defective acute transforming feline retrovirus with oncogene homology to abelson murine leukemia virus (a-mulv) (p. besmer, w.d. hardy,jr., e. e. zuckerman, p. j. bergold, l. lederman, and h. w. snyder, jr. (1983) nature (london) 303, 825-828). in contrast to a-mulv which was isolated from a hematopoietic tumor, the hz2-fesv derives from a multicentric fibrosarcoma. we have molecularly cloned the hz2-fesv provirus from mink hz2-fesv nonpro ... | 1987 | 2884777 |
| mouse retroviral sequences acquired by cell lines after passaging through nude mice detected by hybridization of the fms probe psm3. | the expression of a large rna transcript, 8.5 to 9.5 kilobases, possibly related to the fms oncogene in mouse, rat, and human tumor cells, has been described in the literature. however, the psm3 fms probe used to detect this gene transcript contains a significant amount of the pol gene of the susan mcdonough strain of feline sarcoma virus from which it was derived. using a fms probe which does not contain any viral pol sequences, no such "fms-related" transcripts were detected in cell lines prev ... | 1989 | 2910482 |
| no expression of a rous sarcoma virus-induced tumor antigen in mammalian cells infected with retroviruses transducing other oncogenes of the src gene family. | immunization with mouse and rat cells transformed by rous sarcoma virus (rsv) or by b77 avian sarcoma virus (asv) induced complete transplantation resistance against an rsv-induced mouse tumor (csa1m) in syngeneic hosts. in contrast, most of the mice immunized with a fujinami sarcoma virus-transformed rat fibroblast line (fsv-3y1), a feline sarcoma virus-transformed cat fibroblast line (fesv-fef), an abelson leukemia virus-infected balb/3t3 cell line (ablv-3t3), or an uninfected 3y1 cell line co ... | 1985 | 2985859 |
| a beta-type transforming growth factor, present in conditioned cell culture medium independent of cell transformation, may derive from serum. | an alpha-type transforming growth factor (tgf alpha) is produced at high levels by rat embryo cells transformed by the snyder-theilen strain of feline sarcoma virus (fesv). addition of 2 ng mouse epidermal growth factor (megf) during purification identified the presence of a second, egf-dependent growth factor of the tgf beta type (tgf beta) in this conditioned medium. this factor had an approximate mr of 12,000 and eluted at 37% acetonitrile during high performance liquid chromatography. this e ... | 1985 | 2987278 |
| killer cells of feline leukemia virus- and feline sarcoma virus-infected transformed cells: the role of nk, adcc, and in vitro generated cytotoxic cells. | feline white blood cells (wbc) manifested a primary in vitro mixed lymphocyte tumor cell culture (mltc) proliferative response to feline leukemia virus-feline sarcoma virus (felv-fesv)-infected transformed target cells, which reached a peak at day 15. furthermore, primary in vitro mltc cultures generated cytotoxic killer cells capable of killing a variety of targets in non-major histocompatibility gene complex restricted fashion, and effector cells were capable of killing targets introduced repe ... | 1985 | 2992811 |
| immunological and biochemical characterization of hz2 feline sarcoma virus and abelson murine leukaemia virus translation products. | the extent of homology between the translation products of the hz2 strain of feline sarcoma virus (hz2-fesv) and the abelson murine leukaemia virus (a-mulv) was examined immunologically and biochemically. antiserum prepared against the v-abl-encoded determinants of the a-mulv polyprotein p120gag-abl was also found to precipitate specifically the 98k mol. wt. hz2-fesv protein (p98gag-abl). the basis for this immunological crossreactivity was indicated by the findings that the two proteins had at ... | 1985 | 2993490 |
| isolation and chromosomal localization of the human fgr protooncogene, a distinct member of the tyrosine kinase gene family. | the cell-derived domain of gardner-rasheed feline sarcoma virus (gr-fesv) consists of a gamma-actin- and a tyrosine-specific protein kinase-encoding sequence designated v-fgr. by utilizing a v-fgr probe, it was possible to detect related sequences present at low copy number in dnas of a variety of mammalian species and to isolate a human fgr homologue. comparative studies revealed that this human dna clone represented all but 200 base pairs of v-fgr. analysis of human genomic dna demonstrated th ... | 1985 | 2995972 |
| molecular cloning and characterization of feline cellular genetic sequences homologous to the oncogene of the mcdonough strain of feline sarcoma virus. | the organization within the cat genome of cellular genetic sequences homologous to the viral oncogene v-fms of the mcdonough strain of feline sarcoma virus (sm-fesv) was determined. four cosmid clones containing overlapping v-fms homologous cellular dna inserts representing a contiguous region of cellular dna of approximately 80 kbp in length have been isolated from a feline cosmid gene library. within this region of the cat genome, the c-fms genetic sequences are dispersed over a region of arou ... | 1985 | 3002022 |
| purification and characterization of the feline sarcoma virus tyrosine-specific kinase pp85gag-fes. | the transforming phosphoprotein pp85gag-fes of the snyder-theilen strain of feline sarcoma virus (st-fesv) was purified in a form which exhibits tyrosine-specific kinase activity. cell lysates of st-fesv-transformed mink nonproducer cells were applied to a column of deae-sephacel and eluted with a linear gradient of nacl in phosphate buffer. kinase activity was found uncomplexed (pp85gag-fes) in the flow-through and was eluted with 200 mm nacl in a complex with pp50 and pp90. the flow-through ma ... | 1986 | 3002032 |
| transformation and productive infection of human osteosarcoma cells by a feline sarcoma virus. | | 1971 | 4325140 |
| attempted induction of sarcoma in a cape chacma baboon with the snyder-theilen feline sarcoma virus. | | 1973 | 4197095 |
| characterization of the monomeric and complex-associated forms of the gag-onc fusion proteins of three isolates of feline sarcoma virus: phosphorylation, kinase activity, acylation, and kinetics of complex formation. | the gag-onc fusion proteins of three isolates of feline sarcoma virus (st-fesv, ga-fesv, tp1-fesv) from a stable noncovalent complex with two cellular phosphoproteins, pp90 and pp50. these two phosphoproteins are the same phosphoproteins which have been shown to complex with the transforming proteins of rous sarcoma virus, fujinami sarcoma virus, yamaguchi 73 virus (lipsich et al., 1982), and prcii avian sarcoma virus (adkins et al., 1982). both the monomeric and complex-associated gag-onc fusio ... | 1986 | 2422812 |
| expression of the mammalian c-fes protein in hematopoietic cells and identification of a distinct fes-related protein. | the avian c-fps and mammalian c-fes proto-oncogenes are cognate cellular sequences. antiserum raised against the p140gag-fps transforming protein of fujinami avian sarcoma virus specifically recognized a 92,000-mr protein in human and mouse hematopoietic cells which was closely related in structure to snyder-theilen feline sarcoma virus p87gag-fes. this polypeptide was apparently the product of the human c-fes gene and was therefore designated p92c-fes. human p92c-fes was associated with a tyros ... | 1985 | 2426571 |
| tumor induction in squirrel monkeys by the st strain of feline sarcoma virus. | | 1972 | 4342616 |
| possible immunoenhancement of persistent viremia by feline leukemia virus envelope glycoprotein vaccines in challenge-exposure situations where whole inactivated virus vaccines were protective. | kittens immunized with purified native felv-gp70 or -gp85 envelope proteins developed elisa, but not virus neutralizing, antibodies in their serum to both whole felv and felv-gp70. kittens vaccinated with envelope proteins and infected with feline sarcoma virus (fesv) developed smaller tumors than nonvaccinates, but a greater incidence of persistent retroviremia. similarly, felv-gp70 and -gp85 vaccinated kittens were more apt to become persistently retroviremic following virulent felv challenge ... | 1986 | 3008409 |
| relationship between humoral antibodies and the failure to develop progressive tumors in cats injected with feline sarcoma virus (fsv). | | 1973 | 4360194 |
| simian sarcoma virus: oncogenicity, focus assay, presence of associated virus, and comparison with avian and feline sarcoma virus-induced neoplasia in marmoset monkeys. | | 1973 | 4360159 |
| specific binding of the mononuclear phagocyte colony-stimulating factor csf-1 to the product of the v-fms oncogene. | cells transformed by the mcdonough strain of feline sarcoma virus (sm-fesv) express a v-fms-encoded glycoprotein whose expression at the cell surface correlates with the transformed phenotype. the mouse mononuclear phagocyte growth factor csf-1 specifically binds to sm-fesv-transformed cells at high-affinity sites indistinguishable from those detected on normal feline macrophages. a monoclonal antibody to a v-fms-encoded epitope competed for csf-1 binding to sm-fesv-transformed cells, and chemic ... | 1986 | 3010289 |
| continuing studies of feline sarcoma virus-induced tumors in nonhuman primates. | | 1973 | 4360157 |
| rescue of cell-transforming virus from a non-virus-producing bovine cell culture transformed by feline sarcoma virus. | | 1974 | 4361178 |
| mammalian sarcoma-leukemia viruses. i. infection of feline, bovine, and human cell cultures with snyder-theilen feline sarcoma virus. | | 1974 | 4361179 |
| in vitro isolation and characterization of the ga strain of feline sarcoma virus. | | 1971 | 5003009 |
| st feline sarcoma virus. biological characteristics and in vitro propagation. | | 1971 | 5003010 |
| transformation of baboon cells with feline sarcoma virus. | | 1973 | 4361814 |
| tumours induced in sheep by injecting cells transformed in vitro with feline sarcoma virus. | | 1974 | 4588154 |
| correlation between humoral antibody and regression of tumours induced by feline sarcoma virus. | | 1971 | 4939178 |
| feline sarcoma virus tumor induction in cats and dogs. | | 1971 | 5103501 |
| a new acute transforming feline retrovirus with fms homology specifies a c-terminally truncated version of the c-fms protein that is different from sm-feline sarcoma virus v-fms protein. | the hz5-feline sarcoma virus (fesv) is a new acute transforming feline retrovirus which was isolated from a multicentric fibrosarcoma of a domestic cat. the hz5-fesv transforms fibroblasts in vitro and is replication defective. a biologically active integrated hz5-fesv provirus was molecularly cloned from cellular dna of hz5-fesv-infected fre-3a rat cells. the hz5-fesv has oncogene homology with the fms sequences of the sm-fesv. the genome organization of the 8.6-kilobase hz5-fesv provirus is 5' ... | 1986 | 3018286 |
| localization of the feline sarcoma virus fgr gene product (p70gag-actin-fgr): association with the plasma membrane and detergent-insoluble matrix. | the v-fgr oncogene codes for a unique transforming protein (p70gag-actin-fgr) that contains virus-specific determinants and cell-derived sequences for both a tyrosine-specific kinase domain and an actin domain. we examined the subcellular distribution of the v-fgr protein by immunofluorescence microscopy and various cell fractionation techniques. by immunofluorescence, the v-fgr protein was localized in a diffuse cytoplasmic pattern within transformed cells. the v-fgr protein was not detectable ... | 1986 | 3012121 |
| transformation by the v-fms oncogene product: role of glycosylational processing and cell surface expression. | the effect of glycosylational-processing inhibitors on the synthesis, cell surface expression, endocytosis, and transforming function of the v-fms oncogene protein (gp140fms) was examined in mcdonough feline sarcoma virus-transformed fischer rat embryo (sm-fre) cells. swainsonine (sw), a mannosidase ii inhibitor, blocked complete processing, but an abnormal v-fms protein containing hybrid carbohydrate structures was expressed on the cell surface. sw-treated sm-fre cells retained the transformed ... | 1985 | 3018522 |
| mechanism of carcinogenesis by rna tumour viruses: the rna-and dna-dependent dna polymerase activities of feline sarcoma virus. | | 1971 | 5166273 |