failure to prove arenavirus infection among the small mammals from an endemic area of korean hemorrhagic the light of recent knowledge on a complex of diseases caused by a new group of viruses, arenaviruses, virological studies largely directed toward small field mammals were undertaken during 1973-1974 aiming at etiological clarification of korean hemorrhagic nephrosonephritis (khnn). specimens were collected in an endemic area of khnn located north to northeast of seoul. virus isolation tests with 299 urine specimens and 131 mite pools recovered from small mammals and 14 acute stage sera from ...197612383
[korean hemorrhagic fever (author's transl)]. 197938978
isolation and partial characterisation of a new virus causing acute haemorrhagic fever in zaire. 197765661
aetiological relation between korean haemorrhagic fever and nephropathia epidemica.a close aetiological relation between korean haemorrhagic fever and nephropathia epidemica of scandinavia has often been suggested because of similarities in their clinical picture and epidemiology. this relation has now been confirmed serologically; raised titres of antibody to korean haemorrhagic fever agent and seroconversions have been found by the indirect fluorescent-antibody technique in 20 patients with nephropathia epidemica.197984207
korean haemorrhagic fever in staff in an animal march, 1975, and january, 1978, there were outbreaks of korean haemorrhagic fever (k.h.f.) among staff in the animal laboratory of the clinical research building of tohoku university hospital. 13 doctors and 1 veterinarian had k.h.f., and all except 1 had a mild but typical clinical course. k.h.f. antibody titres were significantly raised in all 14 patients. 12 other doctors working in the same room had no symptoms and no k.h.f. antibody. k.h.f. antibody was also detected in 3 animal technici ...197987777
antigenic relationship of two strains of simian hemorrhagic fever virus.serological comparison of the prototype and an epizootic (corbell) strain of simian hemorrhagic fever virus revealed that the two viruses were serologically similar. the prototype strain differs from the corbell strain in that the latter cannot be cultivated in vitro. serological comparison of the prototype virus grown in tissue culture and its homologous antibody and the prototype and corbell viruses recovered from rhesus monkey serum and their homologous antibodies showed differences and sugge ...1978100652
a serologic survey of pronghorns in alberta and saskatchewan, determine the exposure of free-ranging pronghorns (antilocapra americana ord) to selected pathogens, serum samples were obtained from 33 live-trapped animals from southwestern saskatchewan in 1970, and from 26 and 51 animals from southeastern alberta, in 1971 and 1972, respectively. antibodies were found to the agents of parainfluenza 3, bovine virus diarrhea, eastern and western encephalomyelitis, infectious bovine rhinotracheitis and the chlamydial group. no serologic reactors were found to ...1975167203
[incidence of antibodies to crimean haemorrhagic fever in animals (author's transl)]. 1975803341
the rnas of the defective interfering pichinide virus.a pichinde persitently infected bhk21/13s culture was established in which defective interfering (di) virus continued to be synthesized after cessation of plaque-forming virus replication. this di virus, concentrated from nacl-polyethylene glycol treated tissue culture fluids, was shown to band over a much broader range than standard virus, in either discontinuous or continuous sucrose gradients. the polyacrylamide gel profile of the rnas extracted from standard virus contained six components wi ...1976819626
[studies on the effects of rhtnf-alpha anti-replication against ehfv and hav in vitro].it was demonstrated in this study that rhtnf-alpha can inhibit the replication of epidemic hemorrhagic fever virus and hepatitis a virus in tissue culture. ehfv infection produced strong fluorescence ( - +) on vero-e6 cells after 9 days. in the rhtnf-alpha treated group, however the fluorescence was very weak (- or +/-). rhtnf-alpha also showed anti-hav effects on 2bs cells. the fluorescence intensity on 2bs cells infected by hav was - +, but in the rhtnf-alpha treated group, the fluorescence wa ...19921319852
preliminary human trial of inactivated golden hamster kidney cell (ghkc) vaccine against haemorrhagic fever with renal syndrome (hfrs).an inactivated golden hamster kidney cell culture (ghkc) vaccine against haemorrhagic fever with renal syndrome (hfrs) has been developed in recent years. a monovalent ghkc vaccine (lot 88-17) was prepared with l99 strain of the rat-type hantavirus, adapted in suckling mouse brain, cultivated in ghkc, and inactivated with 0.025% formalin, and a preliminary trial of the vaccine was carried out in a small number of human volunteers with the approval of the ministry of public health, prc, in order ...19921348595
anti-hantavirus antibodies in human sera in indirect immunofluorescence using antigens of hantavirus hantaan and cg 18-20 on vero e6 cells were examined 5,827 samples of sera from 5,299 probands of czechoslovakia. in 49 persons (0.94%) were found antibodies of titres 1:32 and higher. two groups of elderly farm workers showed a cluster of positive individuals amounting to 9.9% and 29.4% respectively. the ratio of positivities in some other, randomly and specifically selected groups was deep below 1%. the partial results for the group of ...19921352317
clinical characteristics of haemorrhagic fever with renal syndrome in children.from january 1988 to september 1989, seven patients (4 girls and 3 boys, aged 3-12 years) with haemorrhagic fever with renal syndrome (hfrs) were hospitalised at the university children's hospital in belgrade. in four patients the disease appeared as a family outbreak, the others were sporadic cases. in six patients the clinical presentation was suggestive of hfrs, as they had fever with headache, myalgia, sore throat and gastrointestinal illness followed by renal abnormalities. however, severe ...19921353981
belgrade virus: a new hantavirus causing severe hemorrhagic fever with renal syndrome in yugoslavia.two biologically and genetically distinct hantaviruses were isolated from blood and urine specimens collected from four yugoslavian patients with clinically severe hemorrhagic fever with renal syndrome (hfrs). viral isolates from three patients, designated strains belgrade 1-3, were distinct from hantaan, seoul, puumala, and prospect hill viruses as determined by plaque-reduction neutralization tests and restriction analysis of enzymatically amplified m-segment fragments. the fourth isolate, cal ...19921351508
[laboratory-confirmed cases of hemorrhagic fever with renal syndrome which occurred in breclav 1989-1990].the authors describe the first three cases with a serologically confirmed hantaviral aetiology in the czech republic. it is the causal agent of haemorrhagic fever with renal syndrome, western type (strain cg 18-20, identical with the puumala prototype). all patients come from a locality where since 1984 very actively a natural focus of hantaviral infection was investigated in small mammals, and where formerly groups of farmers were found with a high herd immunity with hantavirus. the disease was ...19921354571
the hantaviruses, etiologic agents of hemorrhagic fever with renal syndrome: a possible cause of hypertension and chronic renal disease in the united states. 19921350911
[study on natural infection, biting and transovarial transmission of epidemic haemorrhagic fever virus in leptotrombidium (l.) scutellare].in order to clarify the significance of leptotrombidium (l.) scutellare in transmitting ehf, from oct. to nov. 1988, lungs of rodents captured in endemic areas of ehf in shangxi province were taken for detecting ehf antigen by ifat, twenty-six out of 459 lungs were positive (5.7%). six strains of ehfv were isolated from l. (l.) scutellare collected from the rodents. in oct. 1989, 28 apodemus captured from areas without reported cases of ehf were placed on grassland in endemic areas, free l. (l.) ...19921349851
seroprevalence of antibodies to hantaviruses and leptospires in selected italian population groups.a study of hantaviral and leptospiral antibodies in selected population groups was performed. among high risk subjects in the rome area, hantaan antibody was found in mammalogists (10%) and dialysis patients (6%), while none of the trappers, oarsmen, river policemen and firemen studied tested positive for antibodies to hantaviruses. in occupationally-exposed subjects (farmers, rangers, lumbermen, hunters) from rural and densely forested areas of northern italian regions, the prevalence of hantaa ...19921349288
haemorrhagic fever with renal syndrome, virological and epidemiological aspects.haemorrhagic fever with renal syndrome (hfrs) is characterized by fever, headache, abdominal pain, renal dysfunction and various haemorrhagic manifestations. the viruses causing hfrs all belong to the hantavirus genus in the bunyaviridae family. at least three of the different hantaviruses are associated with human disease: hantaan, seoul, and puumala viruses. hfrs is endemic in a belt from norway in the west, through sweden, finland, the soviet union, china, korea to japan in the east. the clin ...19921349231
detection of nephropathia epidemica virus rna in patient samples using a nested primer-based polymerase chain reaction.a nested primer-based polymerase chain reaction was constructed for the detection of puumala virus rna in patient samples. puumala virus rna was detected in cells from the urinary and the respiratory tracts and in peripheral blood mononuclear cells of patients with nephropathia epidemica. after inoculation with nephropathia epidemica patient material on vero e6 cells and propagation for nine passages (4 months), puumala virus rna was detected at every passage. hybridization under high-stringency ...19921349033
retrospective diagnosis of small epidemic of haemorrhagic fever with renal syndrome. 19921348346
[consider diagnosis of nephropathia epidemica even in southern sweden!]. 19921347580
comparison of hantavirus isolates using a genus-reactive primer pair polymerase chain reaction.rna of more than 40 hantavirus isolates, originating from rodents and humans of widely separated geographical areas, was copied to cdna using reverse transcriptase and amplified by polymerase chain reaction (pcr). a genus-reactive oligonucleotide primer pair, flanking a 365 bp region of the g2 glycoprotein gene, was chosen for genus-reactive pcr. dna products were digested with 20 restriction endonucleases and cleavage patterns were analysed. for strains of known sequence, the restriction patter ...19921347558
identification of hantaan virus-related structures in kidneys of cadavers with haemorrhagic fever with renal syndrome.the etiologic agent of haemorrhagic fever with renal syndrome (hfrs), hantaan virus, was first isolated in 1976. since then numerous hantaan-like viruses have been isolated and five serotypes of hantavirus have been recognized. serological studies indicate that these viruses are globally distributed, with each serotype occurring in specific areas. hantaan virus has been intensively studied antigenically, biochemically, and genetically. however there is still a paucity of information on the patho ...19921346088
[detection of hfrs hantaan virus rna sequences by pcr]. 19921357208
typing of hantaviruses from five continents by polymerase chain reaction.hantavirus, a genus in the family bunyaviridae, is comprised of at least four serologically distinct types: hantaan, seoul, puumala and prospect hill. the present communication reports the use of polymerase chain reaction (pcr) for typing 27 independently isolated hantaviruses from 5 different continents. total cellular rna was extracted from virus-infected vero e6 cell monolayers by the acid guanidium thiocyanate-phenol-chloroform method. we have utilized 5 different sets of oligonucleotide pri ...19921332278
effect of neutralizing monoclonal antibodies on hantaan virus infection of the macrophage p388d1 cell line.the effect of neutralizing monoclonal antibodies (n mabs) on hantaan virus infection of macrophages was investigated using p388d1 cells, a murine macrophage cell line. mabs to the g1 protein (g1b) and the g2 protein (g2a and g2c) neutralized viral infectivity in p388d1 cells. n mabs to g1b showed much higher neutralizing potency than those to g2a and g2c. with each n mabs, two distinct effects were observed: neutralization of viral infectivity occurring at high concentrations and enhancement of ...19921284283
bluetongue-like disease of deer.a comparison has been made of the disease produced in white-tailed deer by the viruses of epizootic haemorrhagic disease and bluetongue. the similar nature of these diseases in deer and of some of the viral properties has been described. although these two viruses are considered to be distinct, it is possible by employing an unnatural procedure to produce antibody which will demonstrate a minor antigenic component common to both viruses. biological features that differ between the two viruses ha ...1975169787
epidemic nephropathy in scandinavia is related to korean haemorrhagic fever. 197984099
etiological relation between korean hemorrhagic fever and epidemic hemorrhagic fever in japan.the first case of epidemic hemorrhagic fever in japan was seen in osaka in 1960. the etiologic agent of this disease has not yet been isolated, but a close etiologic relation between korean hemorrhagic fever and epidemic hemorrhagic fever in japan has been suspected because of similarities in the clinical and pathological pictures of the two diseases. this relation has now been confirmed serologically by demonstrating specific immunofluorescent antibodies to korean hemorrhagic fever virus in 19 ...197943726
an emerging zoonosis in scandinavia -- nephropathia epidemica.the paper reviews as zoonosis the disease called nephropathia epidemica in north european countries. the etiology is as yet unsolved but a viral one is highly suspected with small rodents (voles and mice) as the source of infection. based on the epidemiological peculiarities of the disease, the ecology of the agent and hypothetical ways of transmission of infection from small rodents to man are discussed.197720600
complications of nephropathia epidemica: three cases.haemorrhagic fever with renal syndrome (hfrs) in scandinavia is called nephropathia epidemica (ne), and is caused by the puumala-virus, which belongs to the hanta-virus genus. the clinical course of ne is mostly benign, complications are uncommon, and deaths are rarely observed. we report the cases of three patients who developed serious complications in the course of ne caused by the puumala-virus. one patient died within 24 h after admission, another developed progressive neuromuscular dysfunc ...19921353521
cloning and sequencing of puumala virus sotkamo strain s and m rna segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein.the prototype puumala virus (pv) sotkamo strain small (s) and medium (m) rna genome segments were amplified by the polymerase chain reaction (pcr), cloned and sequenced. the s segment is 1830 nucleotides long with an open reading frame coding for 433 amino acids. the identity to the pv hällnäs strain was 83% at the nucleotide and 96% at the amino acid level. the m segment in the sotkamo strain is 3616 nucleotides long and contains one open reading frame of 1148 amino acids with 83% nucleotide an ...19921353107
preparation of candidate vaccinia-vectored vaccines for haemorrhagic fever with renal syndrome.two vaccinia-vectored candidate vaccines for haemorrhagic fever with renal syndrome were prepared by inserting cdna, representing the medium (m) genome segment, or the m and the small (s) genome segments of hantaan virus into the thymidine kinase gene of the connaught vaccine strain of vaccinia virus. in the single recombinant, the m segment was placed under control of the vaccinia virus 7.5 kda promoter. in the double recombinant, the m and s segments were placed under control of the vaccinia v ...19921347189
the prevalence of arboviral, rickettsial, and hantaan-like viral antibody among schoolchildren in the nile river delta of egypt.a serosurvey was conducted during october and november 1989 to estimate the prevalence of selected arboviral, rickettsial, and hantaan viral antibody among a sample of schoolchildren from 4 villages in the bilbeis area of the nile river delta, egypt. blood specimens were obtained from subjects aged 8 to 14 years. enzyme immunoassay testing of the sera indicated that the prevalence of antibody was 9% (21/223) for sicilian sandfly fever, 4% (8/223) for rift valley fever, 3% (15/437) for west nile ...19921363163
selective amplification of cdna sequence from total rna by cassette-ligation mediated polymerase chain reaction (pcr): application to sequencing 6.5 kb genome segment of hantavirus strain b-1.a method, referred to as cassette-ligation mediated polymerase chain reaction (pcr), has been developed to permit selective and specific amplification of cdna sequence from total cellular rna. this technique comprises (i) digestion of cdna with multiple restriction enzymes, (ii) ligation of cleavage products to double-stranded dna cassettes possessing a corresponding restriction site and (iii) amplification of cassette-ligated restriction fragments containing a short, known sequence (but not all ...19921336127
[antibodies against the hantaan virus in italian ex-soldiers stationed in the balkans during world war ii]. 19921283068
[methodical studies on virus isolation in experimental viral hemorrhagic septicemia (vhs) of the trout]. 1977411480
hantavirus isolation from birds. 19921364030
[effect of moxibustion on the immune and anti-virus functions of the rat infected with epidemic hemorrhagic fever virus]. 19921364108
[observation on the therapeutic effect of endoxen (cyclophosphamide) in the treatment of epidemic hemorrhagic fever and an investigation of its pathogenesis (author's transl)]. 197945177
grouping of hantaviruses by small (s) genome segment polymerase chain reaction and amplification of viral rna from wild-caught rats.a single pair of consensus primers in the polymerase chain reaction (pcr) amplified a conserved region of the small genome segment of twenty hantavirus isolates. isolates tested included representatives of the four recognized hantaviruses, hantaan, seoul, puumala and prospect hill, as well as isolates from mus musculus (leakey), bandicota indica (thailand 749), and suncus murinus (thottapalayam). viruses from the nairovirus and phlebovirus genera yielded negative results. the amplification produ ...19921354416
[epidemic nephritis followed by hypogonadism]. 19921366051
hantaan virus infection of human endothelial cells.the primary pathophysiologic finding of the viral disease known as korean hemorrhagic fever, the etiological agent of which is hantaan virus (htv), is vascular instability. to investigate whether htv was able to infect cells derived from human vascular tissue and alter their behavior, we infected in vitro primary adult human endothelial cells from saphenous veins (hsvec). we were able to detect the presence of viral antigens in infected cells both by immunofluorescence and by western blot (immun ...19921356160
stimulation of host-defense mechanism with synthetic adjuvants and recombinant cytokines against viral infection in mice.the efficacy of synthetic immunoadjuvants and recombinant cytokines for the potentiation of host-resistance against virus infection was investigated using mouse models infected with sendai virus and herpes simplex type 1 virus (hsv). the synthetic mdp derivative, mdp-lys(l18), and recombinant cytokines, il-1 beta, ifn-gamma, g-csf and gm-csf were shown to be effective for the stimulation of nonspecific protection against sendai virus infection in mice. both mdp-lys(l18) and gm-csf were effective ...19921329443
[hemorrhagic fever with renal syndrome--etiology].hfrs was confirmed serologically in two patients, who were infected in the natural focus near zupanja. using the bigger number of virus antigens in indirect immunofluorescent antibody test, afterwards was proved the agent could be classified into the serotype 3 of hantaviruses, and antigenically is identical with virus ne (strain hällnäs), respectively yugoslav isolate "vranica". serotype differentiation of the agent in patient who were infected in the area of igman, near sarajevo, has not been ...19911366321
characterization of dobrava virus: a hantavirus from slovenia, yugoslavia.small mammals were collected in natural foci of hemorrhagic fever with renal syndrome (hfrs) in slovenia, yugoslavia, and a hantavirus was isolated from the lungs of an apodemus flavicol lis captured in dobrava village. this new isolate, dobrava virus, was compared with representative strains of the hantavirus genus by serological and polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) methods. it was found by cross immunofluorescent and enzyme-linked immunosorbent assa ...19921360999
[seroprevalence of hemorrhagic fever with renal syndrome in foresters of ile-de-france]. 19921361675
a study of nephropathia epidemica among military personnel in sweden.the incidence of nephropathia epidemica (ne), caused by puumala virus, among military troops operating in an endemic area of sweden was investigated. a total of 705 soldiers (age 18-25) involved in field training in three different endemic counties were bled twice within a 6-month interval. three individuals seroconverted when tested for puumala virus antibodies by igg elisa. symptoms typical of ne were not recorded in any of the patients. however, mild febrile episodes were recorded in 2 of the ...19921355610
hantaanvirus among urban rats from a slum area in bangkok.a total of 106 rodents sera from slum wat phai ton and slum klong toey were examined by immunofluorescent antibody assay during may to august 1990. the positive sera were further tested by plaque reduction neutralization test with the prototype hantaanvirus and the rat-associated hantaan like virus. isolation attempts were also performed from their tissues. antibody-positive rats were found in both slum areas, 32.7% in slum wat phai ton and 5.6% in slum klong toey. rattus norvegicus was the majo ...19921488708
[antibodies against hantaviruses in game and domestic oxen in the czech republic].using the test of indirect immunofluorescence, the authors examined sera of animals for antibodies against the strain cg 18-20 of hantavirus (identical with strain puumala) and against the hantaan strain. as a control and supplement of the examination the authors used cells containing the tbe virus, strain hypr. in a group of 260 hares (lepus europaeus) in 3.5% antibodies were present against hantavirus cg 18-20. of 41 deer (capreolus capreolus) they were present in 14.1%. of 11 fallow-deers non ...19921534516
histopathologic changes of the spleen in suckling rats inoculated with hantaan virus.the purpose of this study is to delineate the histopathologic findings of the spleen after hantaan viral inoculation, which is the largest lymphoid organ in rats, and to identify the viral location by anti-hantaan virus (htnv) monoclonal antibody. all the sixty one suckling rats of less than twenty four hours of age were used. except twenty one rats of control group, twenty-five rats inoculated intracerebrally for the early change and fifteen suckling rats inoculated intramuscularly for the late ...19921355973
[hantavirus infection with acute kidney failure].hantavirus infection was confirmed by history, symptoms and biochemical changes, as well as immunofluorescence test in 29 patients (24 men, 5 women; mean age 36.9 +/- 11.5 years) with nontraumatic renal failure (anf), retrospectively in 15 patients. cardinal symptoms were acute onset (n = 29), fever (n = 27), pain in the flanks, abdomen or head (n = 27), reduced glomerular filtration rate (n = 29), proteinuria (n = 25) and thrombocytopenia (n = 16). normal renal function was restored in all pati ...19921356086
comparison of the deduced gene products of the l, m and s genome segments of hantaviruses.the amino acid sequences deduced from all currently available nucleotide sequences of hantaviruses are compared. comparisons of three large (l), eight medium (m) and five small (s) genome segments are included. a consensus sequence is provided, allowing easy identification of conserved and unique gene regions. the viruses included in this report represent four serologically distinct hantaviruses which are capable of causing severe, moderate, mild or no human disease.19921626424
nucleotide sequence and coding strategy of the uukuniemi virus l rna segment.the complete nucleotide sequence of the l rna segment of uukuniemi virus has been determined from cloned cdna. the l rna is 6423 nucleotides in length, and is of negative polarity. the viral-complementary rna contains a single large open reading frame of 2104 codons which corresponds to the l protein (m(r) 241039). comparison with the l protein sequences of other members of the bunyaviridae showed homology with the rift valley fever phlebovirus l protein (38% amino acid identity), but no detecta ...19921629699
development and application of protein g antibody assay for the detection of antibody to hantavirus.a new serodiagnostic method designated protein-g antibody assay (pga) was developed for detection of hantavirus infection in various species of animals. the assay procedure includes reacting the sera with hantavirus-infected cells on glass slides, followed by incubation of biotinylated protein g and amplification with the avidin-biotinylated peroxidase complex. specific antibody in rabbit, rat, mouse and mongolian gerbil serum was detected by this method. the pga titres were similar to those of ...19921634601
antibody-dependent enhancement of hantavirus infection in macrophage cell lines.antibody-dependent enhancement (ade) of hantavirus infections (strains hantaan 76-118 and sr-11) was studied using macrophage-like cell lines (j774.1, p388d1, and u937). significantly higher virus titers (1,000 to 4,000 ffu/ml) were obtained by pretreatment of the virus with immune serum as compared to normal serum (less than 20 ffu/ml). monoclonal antibodies (mabs) to strain hantaan 76-118 were employed to determine the antigenic determinants responsible for the ade activity. ade of the infecti ...19921370367
complete nucleotide sequence of segment 5 of epizootic haemorrhagic disease virus; the outer capsid protein vp5 is homologous to the vp5 protein of bluetongue virus.the complete nucleotide sequence of a cdna clone representing the segment 5 rna of epizootic haemorrhagic disease virus (ehdv) united states serotype 1 was determined. the 5' and 3' termini of the rna are complementary and are capable of forming secondary structures. the comparison of the predicted amino acid sequence of the encoded outer capsid protein (vp5) with the sequences of vp5 from four serotypes of bluetongue virus, the prototype orbivirus, revealed that the protein shares 59% to 62% ho ...19911662845
[study on the serological typing of patients with epidemic hemorrhagic fever (ehf) and host animals in junan county, shangdong province].using hemagglutination inhibition test (hi), we confirmed that junan county is an endemic area of both ehf of r. norvegicus and a. agrarius, which indicated that the main host animals were r. norvegicus and a. agrarius in the area. remarkable difference was shown between the infectious rates of pigs from the endemic area and that of pigs from the nonendemic area, which suggested that pigs could play certain roles in the transmission of ehf. we also proved that the main transmission mode of ehf v ...19921356628
anti-endothelial cell antibodies in nephropathia epidemica and other viral diseases.increased capillary permeability is a central feature of the severe forms of haemorrhagic fever with renal syndrome (hfrs) and occurs also, though less frequently, in nephropathia epidemica (ne), one of the milder forms of this syndrome, caused by puumala virus. we therefore searched for antiendothelial cell antibodies (aeca) in patients with ne and in those with other presumed or serologically proven acute viral illnesses. by enzyme immunoassay, using human umbilical vein endothelial cells (huv ...19921356668
[a study on virus localization and microcirculation in the liver in patients with epidemic hemorrhagic fever].the findings in our clinical study was consistent with those reported by others that a temporary elevation of serum alanine aminotransferase activity is associated with epidemic hemorrhagic fever (ehf). however, the pathogenesis of the concomitant changes in liver function tests is still not clear. to clarify whether ehf virus is cytopathic or not for the liver, percutaneous liver biopsy was done in 19 patients with ehf within 3-12 days after the onset of symptoms. these liver biopsy specimens w ...19921356713
[a study on transmission of epidemic hemorrhagic fever virus through skin wounds among rodents in natural conditions].224 rodents captured from endemic areas of epidemic hemorrhagic fever (ehf) in hubei province were examined for the presence of skin wounds and ehf viral antigens and antibodies by immunofluorescent assay (ifa) technique. it was found that 30.8% (69/224) of the captured rodents had skin wounds and that 24.6% (17/69) and 29.0% (20/69) of the wounded rodents were positive respectively to ehf viral antigens and antibodies, as compared with the corresponding rates of 9.0% (14/155) and 8.4% (13/155) ...19921356718
serum antibodies to structural proteins of hantavirus arise at different times after enzyme-linked immunosorbent assay (elisa) was developed for the quantification of serum antibodies against group-specific epitopes of the glycoproteins (g1, g2) and nucleoprotein (np) of the genus hantavirus. this assay was used to study the kinetics of the development of serum antibodies after natural infection with puumala-like virus in humans. to this end a panel of 34 serum samples collected from individuals at different times after natural infection was tested by the elisa. the samples w ...19921357082
[leptotrombidium scutellare in transmission of epidemic hemorrhagic fever virus].to further elucidate the role of leptotrombidium scutellare in transmission of epidemic hemorrhagic fever virus (ehfv), a series of studies were carried out from 1988 to 1990. ehfv was isolated from both larvae of free mites collected from the grassland of endemic areas and larvae of filial mites hatched in the laboratory. the suckling mice bitten by these larvae were infected by ehfv. because only the larvae of chigger mite can bite vertebrate hosts and only take a full meal in its entire life ...19921363219
severe hemorrhagic complications from infection with nephropathia epidemica strain of the following we describe a case of severe hemorrhagic fever with renal syndrome (hfrs) caused by puumala infection. the diagnosis was made by immunofluorescence technique and by solid phase enzyme immunoassay using recombinant nucleocapsid antigen of a puumala serotype strain. such a clinical course with severe bleeding complications is considered untypical for puumala induced hfrs.19921358493
hemorrhagic fever with renal syndrome in yugoslavia: epidemiologic and epizootiologic features of a nationwide outbreak in 1989.a nationwide epidemic of hemorrhagic fever with renal syndrome (hfrs) occurred in yugoslavia in 1989. sera from 609 hospitalized patients, from all six republics (bosnia and hercegovina, croatia, macedonia, montenegro serbia, slovenia) and two provinces (kosovo and vojvodina), who had signs and symptoms suggestive of hfrs, and sera and lung tissues from 544 small mammals belonging to 13 species were studied for evidence of hantavirus infection. of the 226 patients with serologically confirmed hf ...19921363468
pulmonary involvement in nephropathia epidemica as demonstrated by computed a prospective study 19 adult patients with nephropathia epidemica were examined in the acute phase of disease with computed tomography (ct) of the lungs and conventional chest radiography. infiltrates and/or pleural effusions were seen in ten of 19 patients. in two of the patients, abnormalities were disclosed only by ct. patients with pathologic radiography findings had a more pronounced inflammatory response, as measured by c-reactive protein and leukocyte count, than did those with normal ...19921358824
protective role of antigenic sites on the envelope protein of hantaan virus defined by monoclonal investigate the role of hantaan virus envelope glycoprotein in infection, a panel of monoclonal antibodies (mabs) was examined in vitro with several serological tests and in vivo by passive transfer experiments in mice. an antigenic site, specific for the inhibition of infected cell focus was detected with the focus inhibition neutralization test (fint), in addition to the neutralization related antigenic sites, which were revealed by the ordinary focus reduction neutralization test (frnt). s ...19921381911
antibodies to puumala virus in humans determined by neutralization assay for detection of neutralizing antibodies to puumala virus using 96-well microtiter plates (nt-elisa) was developed and evaluated. the test proved to have similar sensitivity and specificity as an igg elisa and indirect immunofluorescence test, when screening 187 sera (with an antibody prevalence rate of 19%) from normal populations in an endemic area of nephropathia epidemica (ne) in sweden. ne-patients monitored for 2 years had neutralizing antibodies in early sera collected 1-4 days a ...19921358908
[comparison study on distribution of two types of hemorrhagic fever with renal syndrome (hfrs)].ifat-positive serum samples were typed by hi and divided into appodemus-type (type a) and rattus-type (type r). the results showed that in pingdu and jimo counties, the patients were mainly of type a, whereas in junan and in tengzhou counties, the patients were mainly of type r. the age distribution showed that 96.0% of the type a patients belonged to age groups between 15-54 years, and no patients were younger than 15 years, or older than 65 years. the age distribution in type r patients, howev ...19921363749
eradication of hantavirus infection among laboratory rats by application of caesarian section and a foster mother technique.hantavirus antibodies were demonstrated by the indirect immunofluorescent antibody assay, in the serum of inbred strains of laboratory rats, during the period 1973-1982, at the unit of experimental immunology in the catholic university of louvain, brussels, belgium. lou rats, as well as immunocytomas, which were requested by laboratories in the u.k. and the netherlands, were supplied at a time when the infection was unknown and unsuspected in europe. hantavirus-infected laboratory rats were rend ...19921358976
[the disposition of natural foci of hemorrhagic fever with renal syndrome in different landscape areas of tyumen province].lungs of 3159 animals of the forest complex from 90 areas of 30 administrative districts of tyumen province were examined by enzyme immunoassays for antigen of hemorrhagic fever with renal syndrome (hfrs) during 5 years, 1985-1989. the antigen of hers virus was detected in the lungs of mammals of 8 species: clethrionomys glareolus and cl. rutilus, siberian and arctic lemmings (first findings in the world), m. oeconomus, field mouse, common and pygmy shrews. nearly all the findings refer to the s ...19921359712
[the use of nephrography in the differential diagnosis of epidemic nephritis]. 19901364680
[the intensity of the immune response to the hemorrhagic fever with renal syndrome virus among different population groups of bashkortostan].for the first time the natural immune stratum to hemorrhagic fever with the renal syndrome (hfrs) among the population of all regions of bashkiria, a large territory with the highest morbidity level in this infection in the ussr, was studied. 9,176 persons over 15 years of age were examined by radioimmune techniques. analysis of the immune structure of the population revealed that the share of immune males was higher than that of immune females, but the difference was less than the difference be ...19921359720
immunity to hantavirus challenge in meriones unguiculatus induced by vaccinia-vectored viral proteins.vaccinia virus recombinants were constructed that incorporated genomic sequences coding for the nucleoprotein (n) and glycoproteins (g1 and g2) of the hantavirus r22 strain isolated from a rat in china, and designated as rnv and rmv9, respectively. the proteins expressed by rnv and rmv9 were identified by radioimmunoprecipitation and indirect immunofluorescence assay using a panel of monoclonal antibodies and polyclonal immune sera, and were found to be antigenically indistinguishable from authe ...19921359802
bank vole monoclonal antibodies against puumala virus envelope glycoproteins: identification of epitopes involved in vole (clethrionomys glareolus) monoclonal antibodies (mabs) against the two envelope glycoproteins (g 1 and g 2) of the puumala (puu) virus were generated and characterized. analyses of the mabs' antigen and epitope specificities showed non-overlapping reactivities of one anti-g 1 and two anti-g 2 mabs. a significant neutralizing activity was shown by the anti-g 1 and one of the anti-g 2 mabs, suggesting the existence of at least one neutralizing domain on each of the two glycoproteins. the ...19921381914
[prevalence of hantavirus infection in small mammals in the south moravian region].in 1987 to 1990 in the south moravian region 1094 lungs of small terrestrial mammals were examined for hantavirus antigen and sera of 525 rodents and 93 hares for antibodies against these viruses. in the majority the materials were not from the same individual. lungs were examined by the elisa method, antibodies were assessed by the indirect immunofluorescence reaction. an antigen resembling the western subtype (puumala) was found in 65 animals (5.9%) of two rodent species (microtus arvalis--63 ...19921387051
acute hantavirus infection. 19911681336
[study of the cultured human endothelial cells infected by epidemic hemorrhagic fever virus].virus antigen could be detected in the cytoplasm of infected human endothelial cells (hec) by immunofluorescent assay (ifa) 2 to 10 days after the inoculation of epidemic hemorrhagic fever virus (ehfv), but no apparent histologic changes could be found by phase contrast light microscopy, as well as no mature virus particles could be detected under the transmission electron microscope. reinoculation of the freeze-melt supernatant of hec 8 days after the inoculation of ehfv to ehfv susceptible ver ...19921477943
hantaan antibody in italian ex-soldiers who served in the balkans. 19911682675
[differential diagnosis in nephropathia epidemica: nephrography or virus serology?]. 19911364468
maturation of hantaan virus glycoproteins g1 and g2.hantaan virus-infected vero e6 cell lysates were used for immunoprecipitation with monoclonal antibodies against glycoprotein g1 (mabg1) or g2 (mabg2). when cell lysates were prepared with buffer containing nonionic detergent, both g1 and g2 glycoproteins were precipitated with either mabg1 or mabg2. in contrast, when cell lysates were prepared with a buffer containing ionic detergents mabg1 precipitated only glycoprotein g1 and mabg2 precipitated only glycoprotein g2. heterodimers and possibly ...19921604818
a comparison of different genomic probes in the detection of virus-specified rna in orbivirus-infected cells.different 32p-labelled genomic probes of bluetongue virus (btv), epizootic haemorrhagic disease virus (ehdv) and equine encephalosis virus (eev) were compared with respect to the detection of virus-specified rna in infected cells. the probe derived from the genome segment that encodes nonstructural protein ns1 was found to be the most sensitive, detecting virus-specified rna in glutaraldehyde-fixed cells as early as 2-3 h p.i. this comparison was based on the observation that the ns1 gene probe ...19911651948
susceptibility of laboratory and wild rodents to rattus or apodemus-type wistar rats (rattus norvegicus), apodemus agrarius, meriones unguiculatus, clethrionomys rufocanus, and apodemus argenteus were inoculated with rattus-type (sr-11, ki-262, and tb-314) or apodemus-type (hantaan 76-118) hantaviruses. production of serum antibody to the inoculated virus (iaha titres of 1:32 to 1:4 096) was obsersved in all rodent species 10 weeks after virus inoculation. rattus-type virus was detected in some organs of all the rodent species employed except of apodemus agrari ...19911683116
[severe form of hemorrhagic fever with renal syndrome. an autochtonous case observed in parisian suburbs]. 19911673238
mechanisms of disease in hantavirus infection: pathophysiology of hemorrhagic fever with renal syndrome.hemorrhagic fever with renal syndrome (hfrs) is an acute viral disease that occurs over wide areas of europe and asia. hantaviruses are the cause of this syndrome. the hallmark of hfrs is the triad of fever, hemorrhage, and renal failure. in its severe form it is associated with significant mortality. the syndrome evolves through five phases: febrile, hypotensive, oliguric, diuretic, and convalescent. the central physiologic derangement in hfrs is vascular dysfunction, manifested by impaired vas ...19911673261
specific puumala igg virus half a century after haemorrhagic fever with renal syndrome. 19911676130
[hantaan virus infection]. 19911672850
[hantaan virus infection in twente].to find out if hantavirus infection, as a cause of acute renal failure, has been overlooked in the past. to determine if hantavirus can be found in wild rodents, particularly in red bank voles (clethrionomys glareolus), living in the area.19911675769
localization and changes of hemorrhagic fever with renal syndrome virus in lymphocyte subpopulation.double-labeled immunocytochemistry was used for studying the localization and distribution of hemorrhagic fever with renal syndrome virus (hfrsv) antigen in the peripheral blood lymphocyte subpopulations in hfrs patients and the quantitative changes of these subpopulations. it was found that hfrsv might infect different subpopulations of lymphocytes. the infection rate of b cells (31.3%) was significantly higher than that of t cells (8.5%), and the infection rate of cd8+ subpopulation (10.5%) wa ...19911680612
nephropathia epidemica (hemorrhagic fever with renal syndrome) in scandinavia.nephropathia epidemica (ne) in scandinavia is a zoonosis caused by puumala virus. the main animal reservoir is the bank vole. ne predominantly affects men. its annual incidence varies in a cyclic fashion, with peaks occurring every third to fourth year. the clinical picture of ne in scandinavia is similar to that of hemorrhagic fever with renal syndrome in other parts of the world, although ne generally has a milder course. the case-fatality rate is approximately 0.2%. the most common clinical f ...19911681581
antibodies to bluetongue and epizootic hemorrhagic disease viruses in a barrier island white-tailed deer population.from 1981 through 1989, serum samples from 855 white-tailed deer (odocoileus virginianus) from ossabaw island, georgia (usa), were tested for antibodies to bluetongue virus (btv) and epizootic hemorrhagic disease virus (ehdv). during this period, prevalence of precipitating antibodies to btv and ehdv as determined by agar gel immunodiffusion (agid) tests decreased from 74% to 3% and from 34% to 1%, respectively. antibodies were detected in serum samples from 0.5-yr-old deer only during 1981, 198 ...19911684621
rapid diagnosis of hantavirus disease with an igg-avidity assay.nephropathia epidemica (ne), due to puumala virus, is endemic in eastern europe and scandinavia. serodiagnosis of ne relies on conventional indirect immunofluorescence to detect igg against puumala virus. however, in conventional serology, most patients with acute ne have raised but stable non-diagnostic antibody titres. for better serodiagnosis of ne, we have developed a test that measures the avidity (functional affinity) of igg antibodies against puumala virus. this new assay was highly speci ...19911682736
the hantaan virus m-segment glycoproteins g1 and g2 can be expressed independently.the two glycoproteins of hantaan virus (htv), g1 and g2, are encoded as a continuous single open reading frame in the m segment of the virion rna. they are believed to be synthesized contemporaneously via a polypeptide precursor which is then processed to yield two glycoproteins, both of which appear in the golgi complex of the cell. to study the properties of g1 and g2 as separate entities, we have constructed vaccinia virus recombinants which contain the sequences for each glycoprotein individ ...19921548747
etiology and epidemiology of hemorrhagic fever with renal syndrome. 19911685201
[endemic occurrence of hemorrhagic fever with renal syndrome in the federal republic of germany]. 19911683080
distribution of hantavirus serotypes hantaan and seoul causing hemorrhagic fever with renal syndrome and identification by hemagglutination inhibition epidemiologic evaluation of patients with hemorrhagic fever with renal syndrome from different locations in the people's republic of china was conducted to define the prevalence of two hantavirus serotypes, seoul (seo) and hantaan (htn). serum specimens were collected between 5 and 14 days after the onset of illness and were tested for antibodies by both hemagglutination inhibition (hi) and plaque reduction neutralization (prn). by the hi test, the geometric mean titer (gmt) of antibodies to ...19911685497
arthropod vectors in the evolution of bunyaviruses.viruses from each genus of bunyaviridae have preferential relationships to the arthropods of only one or two families, i.e. bunyavirus to mosquitoes (culicidae), phlebovirus to sand flies (psychodidae) and mosquitoes, uukuvirus and nairovirus to ticks (ixodidae and argasidae). an exception is genus hantavirus not proven to be transmitted by vectors. within the bunyavirus genus 16 serogroups have been recognized on the basis of their antigenic relationship. based on isolations from the nature eac ...19911683124
haemorrhagic fever with renal syndrome in germany. 19911670692
hantavirus infection and renal failure. 19911671905
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