| antibodies to vesicular stomatitis new jersey type virus in white-tailed deer on ossabaw island, georgia, 1985 to 1989. | from 1985 to 1989, 491 serum samples were collected from white-tailed deer (odocoileus virginianus) on ossabaw island, georgia (usa) and were tested for neutralizing antibodies to new jersey and indiana type vesicular stomatitis viruses. prevalence of antibodies to vesicular stomatitis new jersey (vsnj) virus in deer for the 5-yr period was 43%. prevalence of antibodies differed by year (p less than 0.0001), and was dependent on age class (p less than 0.0001) and location on the island (p less t ... | 1991 | 1661786 |
| the glycoprotein genes and gene junctions of the fish rhabdoviruses spring viremia of carp virus and hirame rhabdovirus: analysis of relationships with other rhabdoviruses. | the nucleotide sequences of the glycoprotein genes and all of the internal gene junctions of the fish pathogenic rhabdoviruses spring viremia of carp virus (svcv) and hirame rhabdovirus (hirrv) have been determined from cdna clones generated from viral genomic rna. the svcv glycoprotein gene sequence is 1588 nucleotides (nt) long and encodes a 509 amino acid (aa) protein. the hirrv glycoprotein gene sequence comprises 1612 nt, coding for a 508 aa protein. in sequence comparisons of 15 rhabdoviru ... | 1996 | 8806175 |
| location of neutralizing epitopes on the g protein of bovine ephemeral fever rhabdovirus. | the surface glycoprotein g is the major neutralizing and protective antigen of bovine ephemeral fever rhabdovirus (befv). twelve neutralizing mabs against befv strain bb7721 were used to select 33 neutralization escape mutants. the mutants had been classified previously into three major antigenic sites (g1-g3) based on their cross-neutralization patterns. the nucleotide sequence of the entire extracellular domain of the g protein gene was determined for all mutants. each contained a single nucle ... | 1998 | 9820132 |
| broad-spectrum virus reduction in red cell concentrates using inactine pen110 chemistry. | the risk of transmission of blood-borne pathogens by transfusion is a persistent problem in medicine. to address this safety issue, inactine pen110 chemistry is being utilized to develop a process for preparing pathogen-reduced red blood cell concentrates (rbcc). the purpose of this study was to characterize the virucidal effectiveness of the inactine pen110 chemistry in full units of rbcc by using a panel of viruses with diverse properties in composition, size and shape. | 2002 | 12437518 |
| infectivity of a human respiratory syncytial virus lacking the sh, g, and f proteins is efficiently mediated by the vesicular stomatitis virus g protein. | to examine the requirements of the human respiratory syncytial virus (hrsv) sh (small hydrophobic), g (attachment), and f (fusion) proteins for virus infectivity and morphology, we used the prototype a2 strain of hrsv to generate a series of cdnas from which (i) the sh open reading frame (orf), (ii) the sh and g orfs, or (iii) the sh, g, and f orfs were deleted. each deleted orf was replaced as follows: the sh orf was replaced with that of green fluorescent protein; the g orf was replaced with t ... | 2003 | 12610153 |
| vesicular stomatitis virus glycoprotein is a determinant of pathogenesis in swine, a natural host. | there are two major serotypes of vesicular stomatitis virus (vsv), indiana (vsiv) and new jersey (vsnjv). we recovered recombinant vsivs from engineered cdnas that contained either (i) one copy of the vsiv g gene (vsiv-g(i)); (ii) two copies of the g gene, one from each serotype (vsiv-g(nj)g(i)); or (iii) a single copy of the g(nj) gene instead of the g(i) gene (vsiv-g(nj)). the recombinant viruses expressed the appropriate glycoproteins, incorporated them into virions, and were neutralized by a ... | 2003 | 12829843 |
| recombinant vesicular stomatitis (indiana) virus expressing new jersey and indiana glycoproteins induces neutralizing antibodies to each serotype in swine, a natural host. | vesicular stomatitis virus (vsv) is the most common cause of vesicular disease outbreaks in livestock throughout the western hemisphere. two major serotypes, indiana and new jersey, cause epidemic disease in pigs, cattle, and horses. we generated recombinant viruses derived from the indiana serotype genome that were engineered to contain and express: (1) a single copy of the glycoprotein gene from the indiana serotype (vsiv-gi); (2) a single copy of the glycoprotein gene from the new jersey sero ... | 2004 | 15364454 |
| biological differences between vesicular stomatitis virus indiana and new jersey serotype glycoproteins: identification of amino acid residues modulating ph-dependent infectivity. | we previously generated recombinant vesicular stomatitis viruses (vsv) based on the indiana serotype genome which contained either the homologous glycoprotein gene from the indiana serotype (vsiv-gi) or the heterologous glycoprotein gene from the new jersey serotype (vsiv-gnj). the virus expressing the gnj gene was more pathogenic than the parental vsiv-gi virus in swine, a natural host (26). for the present study, we investigated the biological differences between the gi and gnj proteins that m ... | 2005 | 15731252 |
| the effect of co- and superinfection on the adaptive dynamics of vesicular stomatitis virus. | in many infectious diseases, hosts are often simultaneously infected with several genotypes of the same pathogen. much theoretical work has been done on modelling multiple infection dynamics, but empirical evidences are relatively scarce. previous studies have demonstrated that coinfection allows faster adaptation than single infection in rna viruses. here, we use experimental populations of the vesicular stomatitis indiana virus derived from an infectious cdna, to show that superinfection dynam ... | 2007 | 16807134 |
| antibody induction by virosomal, mf59-adjuvanted, or conventional influenza vaccines in the elderly. | in a randomized, observer-blind, three-arm, parallel group, multi-centre trial including 386 elderly subjects in four countries, the immunogenicity and safety was studied of three different trivalent inactivated surface antigen (subunit) influenza vaccine types: a conventional subunit influenza vaccine (siv, brand: influvac and two newer vaccines: a mf59-adjuvanted subunit influenza vaccine (adsiv, brand: fluad and a virosomal subunit influenza vaccine (vsiv, brand: invivac. all vaccines were tr ... | 2007 | 18063446 |
| characterization of the full-length genomic sequences of vesicular stomatitis cocal and alagoas viruses. | in brazil and argentina, vesicular stomatitis (vs) is caused by distinct viral strains serologically related to the classical vesicular stomatitis virus indiana (vsiv), namely vs indiana-2 (vsiv-2) and vs indiana-3 (vsiv-3). here we describe the full-length genomic sequences and organization of the prototype strains of vsiv-2 cocal virus (cocv) and vsiv-3 alagoas virus (vsav). these viruses showed similar genomic organizations to vsiv field isolates except that the non-structural c'/c proteins, ... | 2008 | 18512001 |
| virion disruption by ozone-mediated reactive oxygen species. | it is well documented in the scientific literature that ozone-oxygen mixtures inactivate microorganisms including bacteria, fungi and viruses (hoff, j.c., 1986. inactivation of microbial agents by chemical disinfectants. epa 600 s2-86 067. office of water, u.s. environmental protection agency, washington, dc; khadre, m.a., yousef, a.e., kim, j.-g., 2001. microbiological aspects of ozone applications in food: a review. j. food sci. 66, 1242-1252). in the current study, delivery and absorption of ... | 2008 | 18598719 |
| field evaluation of a multiplex real-time reverse transcription polymerase chain reaction assay for detection of vesicular stomatitis virus. | sporadic outbreaks of vesicular stomatitis (vs) in the united states result in significant economic losses for the u.s. livestock industries because vs is a reportable disease that clinically mimics foot-and-mouth disease. rapid and accurate differentiation of these 2 diseases is critical because their consequences and control strategies differ radically. the objective of the current study was to field validate a 1-tube multiplexed real-time reverse transcription polymerase chain reaction (real- ... | 2009 | 19286495 |
| improvement and optimization of a multiplex real-time reverse transcription polymerase chain reaction assay for the detection and typing of vesicular stomatitis virus. | an improvement to a previously reported real-time reverse transcription polymerase chain reaction (real-time rt-pcr) assay for the detection of vesicular stomatitis virus (vsv) is described. results indicate that the new assay is capable of detecting a panel of genetically representative strains of vsv present in north, central, and south america. the assay is specific for vsv and allows for simultaneous differentiation between vesicular stomatitis indiana virus and vesicular stomatitis new jers ... | 2010 | 20453220 |
| genetic and antigenic relationships of vesicular stomatitis viruses from south america. | vesicular stomatitis (vs) viruses have been classified into two serotypes: new jersey (vsnjv) and indiana (vsiv). here, we have characterized field isolates causing vesicular stomatitis in brazil and argentina over a 35-year span. cluster analysis based on either serological relatedness, as inferred from virus neutralization and complement fixation assays, or nucleotide sequences of two separate genes (phosphoprotein or glycoprotein) grouped the field isolates into two distinct monophyletic grou ... | 2011 | 21830071 |
| stat2/irf9 directs a prolonged isgf3-like transcriptional response and antiviral activity in the absence of stat1. | evidence is accumulating for the existence of a signal transducer and activator of transcription 2 (stat2)/interferon regulatory factor 9 (irf9)-dependent, stat1-independent interferon alpha (ifnα) signalling pathway. however, no detailed insight exists into the genome-wide transcriptional regulation and the biological implications of stat2/irf9-dependent ifnα signalling as compared with interferon-stimulated gene factor 3 (isgf3). in stat1-defeicient u3c cells stably overexpressing human stat2 ... | 0 | 25564224 |
| chimeric filoviruses for identification and characterization of monoclonal antibodies. | recent experiments suggest that some glycoprotein (gp)-specific monoclonal antibodies (mabs) can protect experimental animals against the filovirus ebola virus (ebov). there is a need for isolation of mabs capable of neutralizing multiple filoviruses. antibody neutralization assays for filoviruses frequently use surrogate systems such as the rhabdovirus vesicular stomatitis indiana virus (vsv), lentiviruses or gammaretroviruses with their envelope proteins replaced with ebov gp or pseudotyped wi ... | 2016 | 26819310 |
| expression and purification of chicken beta interferon and its antivirus immunological activity. | interferon beta (ifn-β) belongs to a class of natural proteins that can inhibit virus replication. in this paper, nucleic acid sequence encoding chicken interferon beta (chifn-β) mature protein was cloned and highly expressed in escherichia coli (e. coli) by 1mm iptg induction. the expressed chifn-β was about 20% of total bacterial protein. the recombinant protein in form of inclusion body was then solubilised, refolded and purified to a purity of greater than 95% by immobilized metal ion affini ... | 2012 | 22564876 |
| coronins and their role in immunological phenomena. | coronins are a large family of proteins occurring in many eukaryotes. in mammals, seven coronin genes have been identified, evidencing that coronins 1 to 6 present classic coronin structure, while coronin 7 is a tandem coronin particle, without a spiral domain, although the best characterised coronin, in terms of both structure and function, is the mammalian coronin 1. it has been proven that they are related to regulation of actin dynamics, e.g. as a result of interaction with the complex of pr ... | 2016 | 28450807 |
| developing broad-spectrum antivirals using porcine and rhesus macaque models. | isg15-deficient humans exhibit permanent, low-level expression of antiviral effectors that safely protect them from various viruses. because the murine isg15 axis functions differently, we identified animal models that recapitulate the human condition for the development of isg15-targeting broad-spectrum antivirals. canine, porcine, and rhesus macaque isg15, such as human isg15, stabilize usp18, a potent inhibitor of type i interferon (ifn)-i. type i interferon-primed isg15-knockout porcine and ... | 2020 | 31637432 |