| transcriptase activity associated with rabies virion. | rabies virion-associated transcriptase activity was investigated in vitro and compared with that of the new jersey serotype of vesicular stomatitis virus. the concentration of detergent that affected 3hgmp incoporation into acid-insoluble material was significantly different for both viruses. vesicular stomatitis virus new jersey required 0.05 to 0.1% nonionic detergent, whereas rabies virion could not be fully activated unless 4 to 5% detergent was used. other optimal conditions were as follows ... | 1977 | 22766 |
| cross-neutralization between vesicular stomatitis virus type indiana and chandipura virus. | using highly potent immune sheep sera, it was possible to demonstrate that: (1) two rhabdoviruses, classified in the vesiculovirus genus on morphological grounds but previously considered unrelated, viz., the vesicular stomatitis virus type indiana (vsv), and chandipura virus (chv), show a low-level, but distinct cross-neutralization. this was, in most combinations, considerably increased by complement. (2) the species of cells used for growing the viruses for immunization and for neutralization ... | 1979 | 40419 |
| rna synthesis by temperature-sensitive mutants of vesicular stomatitis virus, new jersey serotype. | | 1975 | 163530 |
| virion trascriptase activity differences in host range mutants of vesicular stomatitis virus. | three types of conditional lethal mutant were isolated from wild-type vesicular stomatitis virus, new jersey serotype, after mutagenization by 5-fluorouracil: (i) conventional temperature-sensitive (ts) mutants, which form plaques at 31 c but not at 39 c; (ii) conventional host range mutants (hr ce), which grow in bhk but not in secondary chicken embryo cells; and (iii) temperature-dependent host range mutants (td ce), which form plaques both at 31 and 39 c on bhk cells but only at 31 c on chick ... | 1975 | 170423 |
| ecological associations of vesicular stomatitis virus in rural central america and panama. | the principal objective of this study was to determine ecological associations of vesicular stomatitis virus (vsv)- new jersey and vsv-indiana in rural central america and panama. two types of information were linked: the results of neutralizing antibody tests performed on sera from 3,232 lifetime residents of 189 rural study communitities of central america and panama, and ecological characteristics of the study communities as determined from natural resource atlases. the major finding was that ... | 1976 | 188350 |
| pseudotypes of vesicular stomatitis virus with the mixed coat of reticuloendotheliosis virus and vesicular stomatitis virus. | vesicular stomatitis virus (vsv) forms pseudotypes with envelope components of reticuloendotheliosis virus (rev). the vsv pseudotype possesses the limited host range and antigenic properties of rev. approximately 70% of the vsv, indiana serotype, and 45% of vsv, new jersey serotype, produced from the rev strain t-transformed chicken bone marrow cells contain mixed envelope components of both vsv and rev. vsv pseudotypes with mixed envelope antigens can be neutralized with excess amounts of eithe ... | 1977 | 191647 |
| isfahan virus, a new vesiculovirus infecting humans, gerbils, and sandflies in iran. | the characteristics and ecology of isfahan virus, a new vesicular stomatitis virus (vsv) serotype, are described. two strains of the agent were isolated from pools of phlebotomus papatasi collected in iran in 1975. its animal pathogenicity, growth rate, cytopathic effect, and plaque morphology are similar to those of the other vsv serotypes. electron microscopic examination of the virus demonstrated a bullet shape, the presence of truncated particles and maturation at plasma membranes. antigenic ... | 1977 | 192094 |
| interference and rna homologies of new jersey serotype isolates vesicular stomatitis virus and their defective particles. | | 1977 | 193250 |
| in vitro functional analysis of a temperature-sensitive mutant of vesicular stomatitis virus, new jersey serotype, defective in transcription. | | 1978 | 214958 |
| proposed replicative role of the ns polypeptide of vesicular stomatitis virus: structural analysis of an electrophoretic variant. | the structural lesion in the temperature-sensitive mutant e1 of the new jersey serotype of vesicular stomatitis virus has been assigned to the ns protein. although the packaged wild-type and mutant ns proteins were similarly phosphorylated, the mutant ns protein migrated faster than the wild-type ns protein in polyacrylamide slab gels electrophoresed in the presence of sodium dodecyl sulfate. the resolution appears to be the result of conformational rather than size differences since the two pro ... | 1979 | 228061 |
| comparative analyses of vesiculovirus proteins utilizing partial cleavage fragments at tryptophan residues. | | 1979 | 442541 |
| identification and characterization of serine/threonine protein kinase activity intrinsic to the l protein of vesicular stomatitis virus new jersey. | a photoaffinity analogue of atp, 8-azido-adenosine 5'-triphosphate (8-n3atp), was used to probe atp-binding sites in native transcription complexes of vesicular stomatitis virus (vsv) (new jersey serotype). the analogue was found to be a substrate for a serine/threonine protein kinase that phosphorylated both the ns and l proteins of native complexes. the analogue failed to interact with the rna polymerase, another atp-utilizing activity associated with the transcription complex. kinetic analyse ... | 1992 | 1309863 |
| antigenic variation among strains of the new jersey serotype of vesicular stomatitis virus. | four strains of vesicular stomatitis virus--new jersey (hazelhurst, guatemala, panama, and concan) were compared by cross-neutralization and complement-fixation tests. they were indistinguishable by complement-fixation test; however, by plaque-reduction neutralization method, slight antigenic differences were observed between the hazelhurst and the three other strains. it is concluded that these antigenic differences are insufficient to warrant reclassification of vesicular stomatitis virus--new ... | 1979 | 230769 |
| evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to vesicular stomatitis virus in cattle in an enzootic region of mexico. | an elisa was compared with the plaque-reduction serum neutralization (prsn) test, for detection of vesicular stomatitis virus (vsv) antibodies in cattle in a vesicular stomatitis enzootic region of mexico. a total of 325 bovine serum samples were screened for vsv antibodies. the prsn test was performed, using vero cells. the elisa contained gradient-purified vsv indiana (lab strain) and vsv new jersey (hazelhurst) as the antigens. regression analysis and weighted kappa statistic were used to est ... | 1992 | 1316723 |
| biological transmission of vesicular stomatitis virus (new jersey) by simulium vittatum (diptera: simuliidae). | simulium vittatum females were shown to be competent vectors for the new jersey serotype (vsnj) of vesicular stomatitis virus (camp verde strain). seventy percent of females infected intrathoracically transmitted infectious virions in their saliva after a 10-d incubation period. when infected with virus per os, 63% of the flies tested were positive at day 10, and 45% of flies infected in this manner also secreted virus in their saliva by day 9 or 10 after infection. when ingested by s. vittatum ... | 1992 | 1322991 |
| temperature-sensitive mutants of complementation group e of vesicular stomatitis virus new jersey serotype possess altered ns polypeptides. | in vesicular stomatitis virus new jersey serotype polyacrylamide gel electrophoresis was unable to distinguish the polypeptides of the temperature-sensitive (ts) mutants of complementation groups a, b, c, and f from those of the wild-type virus. however, the ns polypeptide of the representative mutant of group e, ts e1, had a significantly greater electrophoretic mobility than that of the wild-type virus ns polypeptide. the electrophoretic mobilities of the ns polypeptides of the three mutants o ... | 1979 | 225557 |
| identification of heat shock protein 70 in the rabies virion. | we investigated a 73-kda polypeptide (p73), a minor component of the rabies virion (hep-flury and era strains), accounting for as much as 1% of total virion proteins. two-dimensional gel electrophoresis and immunoblotting with the antiserum against the heat shock protein 70 (hsp70) demonstrated that p73 was identical to a constitutive type of cellular hsp70. the antiserum also detected p73/hsp70 in the purified virions of other negative-stranded rna viruses, such as vesicular stomatitis virus (n ... | 1992 | 1325709 |
| effect of temperature-sensitive mutation on activity of the rna transcriptase of vesicular stomatitis virus new jersey. | the virion-associated rna transcriptase activity of vesicular stomatitis virus new jersey temperature-sensitive (ts) mutants was assayed in vitro at the permissive (31 degrees c) and restrictive (39 degrees c) temperatures. rna synthesis at 39 degrees c by the rna-negative ts a1 and the rna-positive ts c1 and ts d1 mutants was similar to that of wild-type virus. the rna-negative ts b1 synthesized only small amounts of rna in vitro at 39 degrees c. the three mutants of complementation group e wer ... | 1979 | 225538 |
| interferon induction by viruses. xxi. vesicular stomatitis virus: interferon inducibility as a phylogenetic marker. | forty-five vesiculovirus isolates were systematically compared for their capacity to induce interferon (ifn) in chick embryo cells under conditions such that the maximum (quantum) yield of ifn per cell and the titer of ifn-inducing particles (ifp) could validly be determined. twelve isolates of the new jersey (nj) serotype of vesicular stomatitis virus (vsv) were good inducers, yielding amounts of ifn that ranged in a continuum from 300 to more than 8,000 units per 10(7) cells. these must reflec ... | 1992 | 1331259 |
| nucleotide sequence of the leader rna of the new jersey serotype of vesicular stomatitis virus. | sequence for the leader rna synthesized by the new jersey serotype of vesicular stomatitis virus is presented and its complementary sequence representing the 3'-terminal sequence of the genome rna is deduced. comparison with the leader rna sequence of the serologically distinct indiana strain reveals that the 3'-terminal region of the genomes of two viruses is highly conserved. | 1978 | 214766 |
| coinfection with a rhabdovirus: vesicular stomatitis virus of indiana and new-jersey serotypes. | coinfection of cells with vesicular stomatitis virus (vsv) of indiana and new-jersey serotypes were performed. thermosensitive mutants (ts) of vsv indiana and the wild type strain (+) of new-jersey were used. harvests and titrations were made at permissive(pt) and nonpermissive (npt) temperatures. it was shown that the harvest was mainly composed of one parental-like infectious particles. the dominance of one serotype over the other was shown to be a function of the relative multiplicity of the ... | 1978 | 214003 |
| rebinding of transcriptase components (l and ns proteins) to the nucleocapsid template of vesicular stomatitis virus. | the l and ns proteins of vesicular stomatitis virions (new jersey serotype) were solubilized with triton x-100 and high-salt buffer and recombined with purified nucleocapsids under conditions similar to those used to reconstitute transcriptase activity in vitro. the nucleocapsid-bound l and ns proteins were separated from unbound proteins on a glycerol gradient. the rebinding of l and ns proteins mimics the in vivo binding in that at saturation the ratio of l and ns molecules to n molecules is a ... | 1978 | 212581 |
| properties of the viruses selected during persistent infection of l cells with vsv. | two virus clones, vsv-mp and vsv-sp, were isolated from l cells persistently infected with vsv (new jersey serotype). both clones were more temperature sensitive than the parent virus, vsv-o, and grew more slowly, gave smaller plaques, less c.p.e. and lower virus yields in l cells. unlike the parent virus, both persistent viruses induced interferon production in l cells. stable carrier cultures could be obtained from l cells infected with vsv-sp at low multiplicities without pretreatment with in ... | 1978 | 211193 |
| target antigens for h-2-restricted vesicular stomatitis virus-specific cytotoxic t cells. | cytotoxic thymus-derived lymphocytes from mice infected with vesicular stomatitis virus (vsv) are h-2 restricted and virus specific for the indiana and new jersey strains of vsv. vsv-indiana-immune t cells can lyse target cells infected with the temperature sensitive (ts) mutant ts 045 about 30 times better when target cell infection occurs at the permissive rather than the non-permissive temperature. since this mutant fails to express the glycoprotein at the cell surface when grown at the nonpe ... | 1978 | 210232 |
| in vitro rna transcription by the new jersey serotype of vesicular stomatitis virus. ii. characterization of the leader rna. | the new jersey serotype of vesicular stomatitis virus (vsv) was able to synthesize a small rna (leader rna) approximately 70 bases in length similar to the leader rna synthesized in vitro by the genetically distinct indiana serotype of vsv. also, the new jersey leader rna contained the same 5'-terminal sequence, ppa-c-g, as the indiana leader rna and had a very similar base composition, with 42% amp, 16% cmp, 18.6% gmp, and 23.4% ump. the 3'-terminal sequence of the vsv new jersey genome rna was ... | 1978 | 206725 |
| in vitro rna transcription by the new jersey serotype of vesicular stomatitis virus. i. characterization of the mrna species. | the in vitro rna synthesis by the virion-associated rna polymerase of vesicular stomatitis virus (vsv), new jersey serotype, was compared with that of the serologically distinct indiana serotype of vsv. the new jersey serotype of vsv synthesized five distinct mrna species in vitro, three of which were smaller than the corresponding species synthesized by the indiana serotype of vsv. these included the mrna's coding for the g, m, and ns proteins. by hybridization experiments, virtually no sequenc ... | 1978 | 206724 |
| inhibition of transcription by immunoglobulins directed against the ribonucleoprotein of homotypic and heterotypic vesicular stomatitis viruses. | specific antisera were raised by immunization of rabbits with purified nucleocapsids containing only rna and n protein (ribonucleoprotein, rnp) obtained from vesicular stomatitis (vs) virions of the indiana (vsind) and the new jersey (vsnj) serotypes. the specificity of anti-rnpind serum was demonstrated by selective precipitation of homotypic rnpind devoid of l and ns proteins; anti-rnpind serum also selectively precipitated soluble n protein present in cytoplasm of infected cells, but co-preci ... | 1978 | 203723 |
| tryptic peptide analysis of the structural proteins of vesicular stomatitis virus. | the individual structural polypeptides of vesicular stomatitis virus have been examined by tryptic peptide analysis of 35s-methionine preparations labelled in vivo and 125i-preparations labelled in vitro. isolates of the two classical serotypes of the virus (indiana and new jersey) and of a sub-type of the indiana serotype, brazil virus, were compared. the study showed that the major internal proteins of all three viruses gave similar maps, whereas the surface glycoproteins gave distinct maps th ... | 1978 | 203655 |
| oligonucleotide fingerprints of rna species obtained from rhabdoviruses belonging to the vesicular stomatitis virus subgroup. | the relationships among the genomes of various rhabdoviruses belonging to the vesicular stomatitis virus subgroup were analyzed by an oligonucleotide fingerprinting technique. of 10 vesicular stomatitis viruses, indiana serotype (vsv indiana), obtained from various sources, either no, few, or many differences were observed in the oligonucleotide fingerprints of the 42s rna species extracted from standard b virions. analyses of the oligonucleotides obtained from rna extracted from three separate ... | 1977 | 196099 |
| temperature-dependent host range mutation in vesicular stomatitis virus affecting polypeptide l. | we established previously that the temperature-dependent host range mutant, td ce 3, of vesicular stomatitis virus (vsv) new jersey possesses temperature-sensitive rna transcriptase activity. in this paper, we describe dissociation and reconstitution experiments designed to determine which vsv polypeptide is affected by the td ce 3 mutation. wild-type vsv new jersey (ts+), the temperature-dependent host range mutant (td ce 3), and the revertant of this mutant (td ce/r1) were used. transcribing n ... | 1977 | 194060 |
| [a genetic study of the interaction of piry virus with drosophila]. | piry virus mutants selected for altered growth properties in drosophila melanogaster have previously been designated agd mutants. this paper presents a classification scheme for grouping some of these mutants and also presents the characteristics of some members of each group. analysis, for each agd clone, of the mean incubation time at 20 degrees c relative to the mean incubation time at 28 degrees c (t20,t28), compared to the range of similar data for wild-type virus enabled us to define 4 cla ... | 1991 | 1796217 |
| heterotypic exclusion between vesicular stomatitis viruses of the new jersey and indiana serotypes. | co-infection of cells with vesicular stomatitis viruses of the indiana and new jersey serotypes results in interference. using specifically-labelled immunofluorescent antibodies, it was demonstrated that within any one co-infected cell, one virus serotype replicated to the relative exclusion of the other serotype. this result was further substantiated by an examination of the virus serotypes released by infectious centres co-infected with both viruses. dominance of one serotype over the other wa ... | 1977 | 192845 |
| role of sialic acid in infection with vesicular stomatitis virus. | the role of sialic acid in the infection of tissue culture cells and mice with vesicular stomatitis virus has been studied. no loss of infectivity of the indiana serotype of the virus was detected by incubating with neuraminidase although the virus particles had lost sialic acid as judged by their ability to inhibit the agglutination of red blood cells by influenza virus. the results did not depend on the type of cell used for growth and assay of the virus since essentially similar findings were ... | 1977 | 192844 |
| cloning and expression of the vesicular stomatitis virus phosphoprotein gene in escherichia coli: analysis of phosphorylation status versus transcriptional activity. | the phosphoprotein (p, previously known as ns) genes of vesicular stomatitis virus serotypes new jersey and indiana have been cloned in the escherichia coli expression vector pet-3a. transcription of p genes in these clones initiated from a phage t7 rna polymerase promoter, whereas translation was driven by the shine-dalgarno sequence and the initiator aug codon of the t7 gene 10 message. the clones were introduced into an appropriate e. coli strain in which t7 rna polymerase was expressed under ... | 1991 | 1848304 |
| cell-free translation of rna synthesized in vitro by a transcribing nucleoprotein complex prepared from purified vesicular stomatitis virus. | the rna species synthesized in vitro by a transcribing nucleoprotein (tnp) complex of vesicular stomatitis virus (vsv) were translated with high efficiency in a fractionated cell-free system derived from reticulocytes. the use of tnp complexes isolated from vsv indiana, vsv new jersey, and chandipura viruses showed that in each case the predominant polypeptides synthesized had electrophoretic mobilities identical to their virion n, ns, and m polypeptides in proportions reflecting those found in ... | 1977 | 191633 |
| synthesis of rna by mutants of vesicular stomatitis virus (indiana serotype) and the ability of wild-type vsv new jersey to complement the vsv indiana ts g i-114 transcription defect. | the ability of certain vesicular stomatitis virus (vsv; indiana serotype) temperature-sensitive (ts) mutants to synthesize intracellular viral complementary rna (vcrna) at permissive or nonpermissive temperatures for productive infections has been investigated. mutants belonging to complementation groups ii, iii, and v synthesize rna at nonpermissive temperature in amounts essentially equivalent to that obtained at permissive temperatures. mutant ts g i-114 possesses a thermolabile transcriptase ... | 1976 | 185410 |
| nucleotide sequence analysis of the l gene of vesicular stomatitis virus (new jersey serotype): identification of conserved domains in l proteins of nonsegmented negative-strand rna viruses. | we have determined the nucleotide sequence of the l gene of vesicular stomatitis virus (vsv), new jersey serotype (ogden strain) by primer extension dideoxy sequencing of the genomic rna with reverse transcriptase. this analysis completes the entire genomic sequence of the vsvnj (ogden). comparison of the deduced amino acid sequence of this l protein with those reported for l proteins of indiana serotype and hazelhurst strain of new jersey serotype revealed an extensive sequence similarity among ... | 1990 | 2155516 |
| serological monitoring of vesicular stomatitis new jersey virus in enzootic regions of costa rica. | the activity of vesicular stomatitis viruses was monitored on 3 dairy farms in costa rica. antibody levels were measured and clinical disease monitored in 165 cattle during a 20 month period (1986-1988). vesicular stomatitis new jersey (vs nj) virus was shown to be enzootic on these farms by a 94.2% prevalence of neutralizing antibody; this did not vary significantly between herds. the mean prevalence of antibody to vesicular stomatitis indiana (vs in) virus was 15.2%, but was significantly high ... | 1990 | 2156464 |
| trajectory analysis of winds and vesicular stomatitis in north america, 1982-5. | outbreaks of vesicular stomatitis, serotype new jersey, during epidemics in the united states and northern mexico, 1982-5, were examined by backward trajectories of winds to investigate spread and possible sources. the outbreaks selected for analysis did not involve introduction of disease by infected animals. the findings indicate that wind could have been responsible for carrying infection from northern mexico to arizona and new mexico and thence to colorado and utah and on to wyoming, idaho a ... | 1990 | 2157606 |
| quantitative studies on adsorption, elution, and haemagglutination of vesicular stomatitis virus. | radioactively-labelled vsv, both indiana and new jersey serotypes, were rapidly and efficiently adsorbed onto goose erythrocytes, but only within a narrow range of ph and at low temperatures. under optimal conditions for adsorption, both infectivity and haemagglutinating activity of vsv were reduced. a basic difference between the two serotypes was identified: adsorption and haemagglutination of the indiana serotype were stable even at ph 9.0 and at 37 degrees c, whereas new jersey serotype was ... | 1976 | 183632 |
| physical properties of new jersey serotype of vesicular stomatitis virus and its defective particles. | the wild-type new jersey serotype of vesicular stomatitis virus generated two types of defective interfering t-particles. the physical properties of these particles and the wild-type virion were determined by laser light scattering spectroscopy, sedimentation measurements, and electron microscopy. | 1975 | 165488 |
| isolation of arboviruses from culicoides midges (diptera: ceratopogonidae) in colorado during an epizootic of vesicular stomatitis new jersey. | an arbovirus survey was conducted in colorado and utah during an epizootic of vesicular stomatitis new jersey (vsnj) that occurred in the western united states in 1982-1983. from 120,422 insect specimens assayed, 106 viruses were isolated. four were rhabdoviruses (vsnj), three were orbiviruses (bluetongue serotype-11), 92 were bunyamwera group (65 main drain and 27 lokern), and seven were simbu group (buttonwillow) bunyaviruses. culicoides spp. accounted for 105 viral isolates (c. (selfia) spp., ... | 1990 | 2167371 |
| classification of the new jersey serotype of vesicular stomatitis virus into two subtypes. | we propose a reclassification of five strains of the new jersey serotype of vesicular stomatitis virus into two subtypes designated concan and hazelhurst. this subclassification into two subtypes is based on reciprocal differences in antibody neutralization of virion infectivity, nucleotide base sequence homology, oligonucleotide maps of virion rna, and interference by defective-interfering particles. | 1978 | 74423 |
| inhibition of viral transcriptase by immunoglobulin directed against the nucleocapsid ns protein of vesicular stomatitis virus. | in search of an anti-transcriptase, antibody was raised in rabbits to partially purified, soluble ns protein present in cytoplasmic extracts of cells infected with the indiana serotype of vesicular stomatitis (vsind) virus. this antiserum gave specific reactions of identity by agar immunodiffusion with both cytoplasmic and virion ns protein. ns antiserum also preferentially precipitated ns 3-h-labeled protein from infected cytoplasmic extracts, whereas anti-whole vsind virion serum also precipit ... | 1975 | 49440 |
| ultrastructural aspects of replication of the new jersey serotype of vesicular stomatitis virus in a suspected sand fly vector, lutzomyia shannoni (diptera: psychodidae). | transmission electron microscopy was used to examine replication of the new jersey serotype of vesicular stomatitis virus (vsnj) (rhabdoviridae: vesiculovirus) in lutzomyia shannoni (diptera: psychodidae), a recently implicated sand fly vector. following ingestion of an infectious blood meal, female sand flies were fixed and examined at approximately 12-hr intervals for six days. the new jersey serotype of vesicular stomatitis virus was first detected in the abdominal midgut after 34 hr of incub ... | 1992 | 1311534 |
| terminal sequences of vesicular stomatitis virus rna are both complementary and conserved. | the nucleotide sequences at the 5' and 3' termini of rna isolated from the new jersey serotype of vesicular stomatitis virus [vsv(nj)] and two of its defective interfering (di) particles have been determined. the sequence differs from that previously demonstrated for the rna from the indiana serotype of vsv at only 1 of the first 17 positions from the 3' terminus and at only 2 of the first 17 positions from the 5' terminus. the 5'-terminal sequence of vsv(nj) rna is the complement of the 3'-term ... | 1979 | 232169 |
| titers of vesicular stomatitis virus, new jersey serotype, in naturally infected male and female lutzomyia shannoni (diptera: psychodidae) in georgia. | seven isolates of the new jersey serotype of vesicular stomatitis (vsnj) virus were obtained from pooled specimens of phlebotomine sand flies, lutzomyia shannoni dyar, collected on ossabaw island, chatham county, ga., in 1989 and 1990. three isolates, including two from males, were obtained from light-trapped sand flies in 1989. four isolates were obtained from pools of sand flies collected from hollow trees in 1990. three of the latter pools contained from 4.0 to 4.7 log10 of plaque-forming uni ... | 1992 | 1322992 |
| sequences of vesicular stomatitis virus rna in the region coding for leader rna, n protein mrna, and their junction. | the rnas extracted from purified preparations of the indiana and new jersey serotypes of vesicular stomatitis virus were polyadenylylated in vitro by using polynucleotide phosphorylase and sequence determination was carried out by the dideoxynucleotide method using reverse transcriptase and dt8ac primer. on both virus rnas a short stretch of adenylic acid residues is present between the regions coding for the leader and n protein mrnas. other features of the rna sequences of the two viruses are ... | 1979 | 228265 |
| phosphorylation of specific serine residues within the acidic domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro. | the phosphorylated state of the vesicular stomatitis virus phosphoprotein (p), an essential component of the virion-associated rna polymerase complex, has been shown to be important for the transcriptional activity of the complex. recent studies indicate that phosphorylation within the acidic domain of the p protein by cellular casein kinase ii is necessary for its activity. in an attempt to identify the exact location of the cell kinase-mediated phosphorylation, we altered specific serine and t ... | 1992 | 1326645 |
| restricted replication of vesicular stomatitis virus in t lymphocytes is coincident with a deficiency in a cellular protein kinase required for viral transcription. | vesicular stomatitis virus (vsv) fails to replicate in mouse t lymphocytes unless the cells have been mitogenically stimulated with concanavalin a (con a). we have examined the possibility that the failure of vsv to replicate in unstimulated t lymphocytes can be attributed to a deficiency in a host protein kinase which activates the viral p protein by phosphorylation, thus rendering it transcriptionally competent. soluble extracts were prepared from purified mouse t lymphocytes, with or without ... | 1992 | 1335023 |
| point mutations in glycoprotein gene of vesicular stomatitis virus (new jersey serotype) selected by resistance to neutralization by epitope-specific monoclonal antibodies. | antigenic variants of the new jersey serotype of vesicular stomatitis virus (vsv-nj) were isolated and cloned by selecting virus plaques resistant to neutralization by high-titered monoclonal antibodies (mabs) directed to glycoprotein (g) epitopes v, vi, vii, or viii. the g proteins of each neutralization-resistant virus variant also exhibited markedly reduced antigenic reactivity with each corresponding epitope-specific mab as determined by enzyme-linked immuno-absorbent assay and by western bl ... | 1988 | 2451346 |
| disulfide-bonded discontinuous epitopes on the glycoprotein of vesicular stomatitis virus (new jersey serotype). | intrachain disulfide bonds between paired cysteines in the glycoprotein (g) of vesicular stomatitis virus (vsv) are required for the recognition of discontinuous epitopes by specific monoclonal antibodies (mabs) (w. keil and r. r. wagner, virology 170:392-407, 1989). cleavage by staphylococcus aureus v8 protease of the 517-amino-acid vsv-new jersey g protein, limited to the glutamic acid at residue 110, resulted in a protein (designated gv8) with greatly retarded migration by polyacrylamide gel ... | 1992 | 1374811 |
| myristylation of the envelope glycoprotein of vesicular stomatitis virus. | the envelope glycoprotein g of the indiana serotype of vesicular stomatitis virus was modified not only by palmitylation, but also by myristylation, both occurring at the carboxy-terminal segment. when infected chinese hamster ovary cells were grown in medium containing [3h]-myristate, the g protein and ga2, the membrane-anchoring fragment containing about 71 amino acids, were labeled. this was shown by immunoprecipitation of cell lysates or extracellular fractions of infected cultures. thin-lay ... | 1991 | 1645706 |
| antibodies to vesicular stomatitis new jersey type virus in white-tailed deer on ossabaw island, georgia, 1985 to 1989. | from 1985 to 1989, 491 serum samples were collected from white-tailed deer (odocoileus virginianus) on ossabaw island, georgia (usa) and were tested for neutralizing antibodies to new jersey and indiana type vesicular stomatitis viruses. prevalence of antibodies to vesicular stomatitis new jersey (vsnj) virus in deer for the 5-yr period was 43%. prevalence of antibodies differed by year (p less than 0.0001), and was dependent on age class (p less than 0.0001) and location on the island (p less t ... | 1991 | 1661786 |
| development and evaluation of an enzyme-linked immunosorbent assay for detection, typing, and subtyping of vesicular stomatitis virus. | an indirect sandwich enzyme-linked immunosorbent assay (elisa) has been used for vesicular stomatitis virus (vsv) typing using sets of monovalent and polyvalent rabbit/guinea pig antisera for identification of vsv types new jersey (vnj) and indiana (vind). the vind polyvalent antiserum (vind-p) detects any strain of the 3 subtypes of the vind type (vind-1, vind-2, and vind-3) with the same strong reactivity. it is also possible to subtype the vind strains using vind-p rabbit antiserum as capture ... | 1991 | 1662076 |
| glycoprotein evolution of vesicular stomatitis virus new jersey. | a t1 ribonuclease fingerprinting study of a large number of virus isolates had previously demonstrated that considerable genetic variability existed among natural isolates of the vesicular stomatitis virus (vsv) new jersey (nj) serotype [s.t. nichol (1988) j. virol. 62, 572-579]. based on these results, 34 virus isolates were chosen as representing the extent of genetic diversity within the vsv nj serotype. we report the entire glycoprotein (g) gene nucleotide sequence and the deduced amino acid ... | 1989 | 2536983 |
| two domains distantly related to protein-tyrosine kinases in the vesicular stomatitis virus polymerase. | we have carried out an exhaustive search for amino acid sequence similarities between vesicular stomatitis virus (vsv) proteins and database entries. unexpectedly, we found that the l polymerase protein contains two blocks of sequence (residues 725-1102 and 1291-1671) with distant but statistically significant similarity to the catalytic domain of tyrosine-specific protein kinases. the first kinase-like region is most similar to members of the abl subfamily, fes and fps (26.6% and 27.3% identity ... | 1989 | 2549720 |
| phlebotomine sand flies as vectors of vesiculoviruses: a review. | phlebotomine sand flies appear to be involved in the epizootiology of 10 of the 16 currently recognized vesiculoviruses. evidence in support of this includes isolation of viruses from wild-caught male and female sand flies; demonstration of oral infection, replication, and bite transmission of viruses by sand flies; the temporal and/or spatial association between infected sand flies and infected vertebrates; and demonstration of transovarial virus transmission. the present review summarizes the ... | 1991 | 1668680 |
| lutzomyia shannoni (diptera: psychodidae): a biological vector of the new jersey serotype of vesicular stomatitis virus on ossabaw island, georgia. | the new jersey serotype of vesicular stomatitis virus (vsnj) is enzootic on ossabaw island, georgia. lutzomyia shannoni is the only phlebotomine sand fly present on the island and there is strong evidence that it is a vector of the virus at this site. this overview summarizes the studies that have been done on the island, reviews the evidence which confirms that l. shannoni is a biological vector of vsnj, and discusses remaining unknown aspects of the epizootiology of vsnj. | 1991 | 1668681 |
| spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates. | strains of vesicular stomatitis virus, new jersey serotype (vsv-nj), isolated from diseased cattle or swine were examined by genomic rna sequencing for genetic diversity potentially leading to antigenic variations in their type-specific glycoproteins as determined by reactivity with epitope-specific monoclonal antibodies (mabs). seven field isolates recovered in colorado, new mexico, georgia, and mexico during the widespread 1982-1985 epizootic in the western united states resembled the prototyp ... | 1990 | 1688475 |
| vesicular stomatitis new jersey virus glycoprotein gene sequence and neutralizing epitope stability in an enzootic focus. | vesicular stomatitis new jersey (vs nj) virus is capable of undergoing rapid evolution in nature and therefore has the potential for antigenic variation. we selected an area of costa rica where vs nj virus is enzootic to study whether this virus used the mechanism of antigenic variation to persist in nature. three sentinel herds and three nonsentinel herds were observed from 1986 to 1988. eleven vs nj virus isolates were collected from naturally infected cattle. remarkably, nine animals that wer ... | 1990 | 1693802 |
| cytolytic t lymphocytes from the balb/c-h-2dm2 mouse recognize the vesicular stomatitis virus glycoprotein and are restricted by class ii mhc antigens. | balb/c-h-2dm2 mice (h-2kdi-adi-eddd), a congenic strain of balb/c mice, have a deletion of the class i mhc ag, h-2ld. this gene encodes the exclusive class i mhc-restricting gene product for vesicular stomatitis virus-specific cytolytic t lymphocytes. when dm2 mice were immunized with infectious vesicular stomatitis virus, a specific ctl response was generated. these ctl lysed vsv-infected targets that expressed iad gene products, but not vsv-infected iad- targets. the ctl were used initially as ... | 1990 | 1695651 |
| effect of glycosylation on the conformational epitopes of the glycoprotein of vesicular stomatitis virus (new jersey serotype). | the conformational epitopes reactive with neutralizing monoclonal antibodies (mabs) appear to be clustered at the middle third of the glycoprotein (g) of the new jersey serotype of vesicular stomatitis virus (vsv-nj) and are flanked by two n-linked carbohydrate chains (w. keil and r.r. wagner, virology 170, 392-407, 1989). we report here studies on the effect of glycosylation on the reactivity of vsv-nj g protein derived from released virions or immunoprecipitated from pulse-labeled cells was no ... | 1991 | 1701943 |
| in vitro synthesis of large rnas by an unusual defective interfering particle of vesicular stomatitis virus. | we analyzed an unusual defective interfering (di) particle of the new jersey serotype of vesicular stomatitis virus. in vitro transcription experiments demonstrated that this di particle was unusual in that it transcribed large rnas in addition to the standard di leader rna. s1 nuclease mapping assays indicated that the large transcripts probably resulted from polymerase reinitiation downstream of the di leader termination site. a sequence related to the indiana serotype putative polymerase prom ... | 1988 | 2833624 |
| the functional domains of the phosphoprotein (ns) of vesicular stomatitis virus (indiana serotype). | a full-length cdna clone of the mrna encoding the phosphoprotein (ns) of the indiana serotype of vesicular stomatitis virus was inserted into the sp6 transcription vector. by in vitro transcription of the inserted gene followed by translation of the mrna in a rabbit reticulocyte lysate, ns protein was synthesized. the biological activity of the protein was demonstrated by rna synthesis in vitro by reconstitution with l protein and n-rna template purified from virions. using oligonucleotide-direc ... | 1988 | 2845648 |
| an enzyme-linked immunosorbent assay for the detection of vesicular stomatitis virus antigen. | an indirect, sandwich enzyme-linked immunosorbent assay (elisa) has been developed for the laboratory diagnosis of vesicular stomatitis (vs). the assay which uses rabbit and guinea-pig antisera to the purified glycoprotein antigens of serotypes new jersey (vsv-nj) and indiana (vsv-ind-1), has both high sensitivity and specificity, but has lower reactivity for the two other indiana subtypes vsv-ind-2 and vsv-ind-3. | 1988 | 2852872 |
| viruses transmitted by sandflies in italy. | five different phlebotomus-transmitted viruses have been isolated in italy. four of them belong to the bunyaviridae family (genus phlebovirus). sandfly fever sicilian and sandfly fever naples viruses were isolated from sick persons during world war ii, toscana and arbia viruses have been isolated more recently from phlebotomus perniciosus and p. perfiliewi. radi virus is the only phlebotomus-transmitted virus isolated in italy from p. perfiliewi, which belongs to the rhabdoviridae family (genus ... | 1991 | 1841253 |
| arbovirus isolations from mosquitoes collected during and after the 1982-1983 epizootic of western equine encephalitis in argentina. | mosquitoes were collected in santa fe and rio negro provinces, argentina, in 1982-1983 during a western equine encephalitis (wee) epizootic. totals of 153,084 mosquitoes from santa fe province and 484 from rio negro province were tested for virus in 2,351 pools. seventeen virus strains were isolated, all from santa fe collections, as follows: 4 wee, 6 venezuelan equine encephalitis, 1 st. louis encephalitis, 2 antequera, 1 maguari, 1 melao, 1 new vesiculovirus (calchaqui), and 1 gamboa. the wee ... | 1987 | 2880521 |
| a newly recognized vesiculovirus, calchaqui virus, and subtypes of melao and maguari viruses from argentina, with serologic evidence for infections of humans and horses. | in 1983, 17 virus strains were isolated from mosquitoes collected during an outbreak of western equine encephalitis in santa fe province, argentina. strains of western equine encephalitis, venezuelan equine encephalitis, st. louis encephalitis, and antequera viruses were isolated, as were several bunyaviruses of the california and bunyamwera serogroups and a new vesiculovirus. complement fixation and neutralization tests were used to identify the california serogroup virus as a subtype of melao ... | 1987 | 2880522 |
| intracellular formation of two soluble glycoproteins in bhk cells infected with vesicular stomatitis virus serotype new jersey. | infection of bhk 21 cells with vsv serotype new jersey gave rise to three intracellular viral glycoproteins: the membrane-integrated g protein and the two soluble glycoproteins gs and gss which lacked the cytoplasmic and transmembrane domains as was deduced from limited chemical cleavage of the glycoproteins by hydroxylamine. both soluble glycoproteins were completely protected by the microsomal membrane against proteolytic digestion. the soluble glycoproteins were formed in the endoplasmic reti ... | 1991 | 1846493 |
| isolation of vesicular stomatitis virus new jersey serotype from phlebotomine sand flies in georgia. | vesicular stomatitis virus new jersey serotype (vsnj virus) was isolated from 6 of 610 pools of phlebotomine sand flies (lutzomyia shannoni) collected on ossabaw island, ga. all isolates were from non-blooded females. infected sand flies were collected at 6 sites at 5 separate times from 3 june through 25 july 1988. thirty-five pools of culicoides ssp. and 48 pools of mosquitoes obtained in conjunction with the infected sand flies also were evaluated for vsnj virus; all were negative. concomitan ... | 1990 | 1971158 |
| [frequency of neutralizing antibodies to the vesiculovirus piry, in blood donors of uberaba, minas gerais, brazil]. | thirteen (8.0%) of 162 blood donors from uberaba-minas gerais state showed neutralizing antibodies to the vesiculovirus piry. the previous residence in rural area (p less than 0.0001) and in the cities of the são paulo state (p less than 0.05) were statistically more frequent in the piry seropositive group. | 1990 | 2135375 |
| phosphoprotein and nucleocapsid protein evolution of vesicular stomatitis virus new jersey. | the entire phosphoprotein (p) and nucleocapsid (n) protein gene sequences and deduced amino acid sequences for 18 selected vesicular stomatitis virus isolates representative of the natural genetic diversity within the new jersey serotype are reported. phylogenetic analysis of the data using maximum parsimony allowed construction of evolutionary trees for the individual genes and the combined n, p, and glycoprotein (g) genes of these viruses. virtually identical rates of nucleotide substitutions ... | 1990 | 2159527 |
| serologic surveillance for vesicular stomatitis virus on ossabaw island, georgia. | seventeen species of mammals and seven species of birds from ossabaw island, georgia, were tested for vesicular stomatitis (vs) neutralizing antibodies. seropositive results were restricted to mammals with six of 17 species testing seropositive for vs (new jersey type) neutralizing antibodies. seropositive species included: raccoons (procyon lotor), white-tailed deer (odocoileus virginianus), feral swine (sus scrofa), cattle (bos taurus), horses (equus caballus), and donkeys (equus asinus). all ... | 1985 | 2987546 |
| vesicular stomatitis virus ns proteins: structural similarity without extensive sequence homology. | the complete nucleotide sequence of the ns mrna of vesicular stomatitis virus (new jersey serotype) was established from two cdna clones spanning the entire coding region of the mrna. the gene is 856 nucleotides long and can code for a polypeptide of 274 amino acids. comparison with the nucleotide sequence of the ns gene of the indiana serotype revealed only 41% sequence homology. the deduced amino acid sequences of the ns proteins were only 32% homologous, with no identical stretches of more th ... | 1985 | 2989560 |
| vesicular stomatitis virus, new jersey serotype: replication in and transmission by lutzomyia shannoni (diptera: psychodidae). | laboratory-reared female sand flies (lutzomyia shannoni) were experimentally infected, orally and by intrathoracic inoculation, with the new jersey serotype of vesicular stomatitis (vsnj) virus. virus replication occurred in the insects following infection by both routes. virus titers greater than 10(4) plaque forming units of vsnj virus were present in heads of orally infected sand flies 12 days after virus ingestion, confirming that a persistent disseminated infection had occurred. both orally ... | 1990 | 2160198 |
| monoclonal antibodies to the matrix protein of vesicular stomatitis virus (new jersey serotype) and their effects on viral transcription. | of 33 hybridomas raised by immunization of balb/c mice with the matrix (m) protein of the new jersey serotype of vesicular stomatitis virus (vsv), 17 secreted monoclonal antibodies (mab) of the igg isotype and, unexpectedly, 16 of the igm isotype. all these monoclonal antibodies bound strongly to vsv-new jersey m protein by elisa, immunoprecipitation, and immunoblotting assays, but exhibited only slight or no cross-reactivity with the m protein of vsv-indiana. four antigenic determinants of vsv- ... | 1985 | 2414913 |
| characterization of new jersey vesicular stomatitis virus isolates from horses and black flies during the 1982 outbreak in colorado. | vesicular stomatitis viruses isolated from horses, afflicted during the recent outbreak in the western united states, and from black flies (simuliidae) were characterized with respect to the homology of their genomic rnas and the mobility of their proteins in polyacrylamide gels. all the isolates were very similar, if not identical, with respect to these two parameters. when the black fly isolate was compared to other vsv isolates, this virus appeared to belong in the hazelhurst subgroup of the ... | 1985 | 2997995 |
| differing responses of hamsters to infection by vesicular stomatitis virus indiana and new jersey serotypes. | intraperitoneal injection of vesicular stomatitis virus, new jersey serotype (vsv-nj), into inbred lsh hamsters resulted in an inapparent infection and survival of the majority of the animals. infectivity titrations of tissues from vsv-nj-infected hamsters showed that little or no virus was present following infection. the few animals that died from vsv-nj succumbed to neurological disease. this is in contrast to our previous work where we found that lsh hamsters are exquisitely sensitive to i.p ... | 1985 | 2414943 |
| recent vesicular stomatitis virus infection detected by immunoglobulin m antibody capture enzyme-linked immunosorbent assay. | we developed an enzyme-linked immunosorbent assay (elisa) that was capable of detecting immunoglobulin m (igm) antibody to vesicular stomatitis virus (vsv) in the sera of experimentally and naturally infected cattle and horses. the detection of igm in the sera of these animals permitted an estimate of the recency of infection by vsv serotype new jersey. a vsv serotype new jersey epizootic strain isolated from a horse and passed once in an aedes albopictus cell line was used to infect a horse and ... | 1985 | 3001132 |
| functional analysis of t lymphocyte subsets in antiviral host defense. | the role of different t cell subsets in antiviral host defense was investigated by treating thymectomized c57bl/6 and cba/j mice with monoclonal rat anti-lyt-2 or anti-l3/t4 igg 2b antibodies 14 and 10 days before infection. this treatment depleted the respective t cell subsets to undetectable levels in peripheral blood when assayed by immunofluorescence. in mice treated with anti-lyt-2, induction of cytotoxic t cells was reduced to less than 1 to 2% after intravenous infection with armstrong st ... | 1987 | 2435794 |
| primary antibody responses to a well-defined and unique hapten are not enhanced by preimmunization with carrier: analysis in a viral model. | we used a viral model to reexamine classical experiments showing that mice previously primed with a "carrier" molecule alone and then challenged with the carrier-hapten conjugate exhibited an enhanced antihapten antibody response. mice were primed with live or uv-inactivated vesicular stomatitis virus (vsv) indiana (ind) serotype with or without complete freund's adjuvant. after challenge with vsv new jersey (nj), these mice developed a secondary-type igg response, measured by antibody binding i ... | 1986 | 3010314 |
| antibodies against the two serotypes of vesicular stomatitis virus measured by enzyme-linked immunosorbent assay: immunodominance of serotype-specific determinants and induction of asymmetrically cross-reactive antibodies. | the serological relationship between the two vesicular stomatitis virus (vsv) strains indiana (vsv-ind) and new jersey (vsv-nj) were analyzed by using an enzyme-linked immunosorbent assay (elisa). immunoglobulin g responses, defined by their resistance to treatment with 2-mercaptoethanol, were assessed by elisa by using sucrose gradient-purified vsv or purified vsv glycoproteins (g) as antigens. when low doses (10(6) pfu) of live vsv or 10(8) pfu of uv-inactivated virus were given intraperitonea ... | 1987 | 2439706 |
| antibodies to vesicular stomatitis new jersey type virus in a population of white-tailed deer. | | 1986 | 3012128 |
| conservation of potential phosphorylation sites in the ns proteins of the new jersey and indiana serotypes of vesicular stomatitis virus. | a full length cdna copy of the ns mrna of the missouri strain (hazelhurst subtype, new jersey serotype) of vesicular stomatitis virus (vsv) has been cloned and sequenced. the mrna is 856 nucleotides long (excluding polyadenylic acid) and encodes a protein of 274 amino acids (mol. wt. 31 000). comparison with the ns gene of the ogden strain (concan subtype, new jersey serotype) showed 15% difference at the nucleotide level and 10% difference at the amino acid level; the majority of the changes we ... | 1986 | 3014048 |
| antibodies to vesicular stomatitis virus in populations of feral swine in the united states. | from 1979 to 1985, 941 feral swine (sus scrofa) from 53 locations in 15 states were serologically tested for antibodies to vesicular stomatitis virus (vsv). antibodies to new jersey serotype vsv were present in 75 swine from five locations in arkansas, florida, georgia, and louisiana. within these populations, antibody prevalences ranged from 10 to 100%. no antibodies to indiana serotype were detected. | 1986 | 3016348 |
| an enzyme-linked immunosorbent assay for the detection of bovine antibodies to vesicular stomatitis virus. | an indirect enzyme-linked immunosorbent assay (elisa) was developed to detect bovine antibody to vesicular stomatitis virus (vsv). serum samples from cows experimentally infected with the new jersey serotype of vsv (vsv-nj) were assayed by the elisa and serum-neutralization (sn) assay. the elisa was as sensitive as the sn assay in detecting bovine antibody to vsv. the correlation between sn titers and elisa values at absorbance at 405 nm was statistically significant. the elisa was not specific ... | 1986 | 3017162 |
| monoclonal antibodies to the glycoprotein of vesicular stomatitis virus (new jersey serotype): a method for preliminary mapping of epitopes. | of the nine antigenic determinants on the glycoprotein (g) of the new jersey serotype of vesicular stomatitis virus (vsv) identified by competitive binding of 25 monoclonal antibodies (mabs), those relegated to epitopes i, ii, iii, and iv exhibited no significant ability to neutralize virus infectivity but some nonneutralizing mabs cross-reacted by elisa with the g protein of vsv-indiana. high-titered neutralization of homotypic virus was exhibited by epitope v, vi, and vii mabs but quite variab ... | 1987 | 2446424 |
| antigen-specific proliferative responses to vesicular stomatitis virus following immunization of cattle with inactive virus. | peripheral blood mononuclear cells (pbm) from four normal cows with no known exposure to vesicular stomatitis virus (vsv) were cultured with a new jersey (nj) serotype (ogden) vsv that had been uv-irradiated and inactivated. pbm from these animals produced no detectable proliferative response when incubated with varying concentrations of vsv-nj (ogden) ranging from 10 ng to 10 micrograms protein/ml. two of these cows were immunized with an experimental vsv-nj vaccine and their pbm were tested at ... | 1989 | 2470191 |
| epitope mapping by deletion mutants and chimeras of two vesicular stomatitis virus glycoprotein genes expressed by a vaccinia virus vector. | deletion mutants and chimeras of the glycoprotein (g) genes of vesicular stomatitis virus serotypes indiana (vsv-ind) and new jersey (vsv-nj) were cloned in plasmids and vaccinia virus vectors under control of the bacteriophage t7 polymerase promoter for expression in cv-1 cells co-infected with a t7 polymerase-expressing vaccinia virus recombinant. truncated and chimeric g proteins expressed by these vectors were tested for their capacity to react with vsv-ind and vsv-nj epitope-specific monocl ... | 1989 | 2471352 |
| interferon induction by viruses. xix. vesicular stomatitis virus--new jersey: high multiplicity passages generate interferon-inducing, defective-interfering particles. | the infectious particles of plaque-derived, low multiplicity passaged wild-type vsv of new jersey origin consistently induce about 1800 units of interferon (ifn)/10(7) aged chick embryo cells. this inducing capacity is sensitive to both uv radiation and heat (50 degrees). virus obtained after two successive high multiplicity passages in gmk-vero cells consistently induced over 25,000 units of ifn/10(7) cells. the ifn induction dose-response curve showed that one ifn-inducing particle (ifp) per c ... | 1989 | 2474895 |
| characterization of the mutations responsible for the electrophoretic mobility differences in the ns proteins of vesicular stomatitis virus new jersey complementation group e mutants. | temperature-sensitive (ts) mutants of vesicular stomatitis virus, new jersey serotype, classified in complementation group e contain lesions in the ns gene, which manifest as marked electrophoretic mobility differences of the mutant ns proteins in sds-polyacrylamide gels. we have cloned full-length cdna copies of the mutant ns mrnas, and have determined their nucleotide sequences. tse1 and tse3 had single nucleotide changes, and tse2 had two nucleotide changes, compared to the wild-type ns gene. ... | 1986 | 3025344 |
| interferon induction by viruses. xviii. vesicular stomatitis virus-new jersey: a single infectious particle can both induce and suppress interferon production. | in contrast to wild-type vesicular stomatitis virus (vsv) of indiana (ind.) origin which express interferon (ifn) inducing- and ifn induction-suppressing activities as mutually exclusive properties, individual particles of wild-type vsv of the new jersey (n.j.) serotype (hazelhurst [h] isolate) paradoxically can both induce ifn and suppress its induction in cells coinfected with a potent inducer of ifn. the properties of ifn induction, and its suppression, appear to reside in the particle that m ... | 1989 | 2477474 |
| epizootic vesicular stomatitis in colorado, 1982: infection in occupational risk groups. | in 1982-1983, an epizootic of vesicular stomatitis occurred in the western united states. veterinarians, research workers, and regulatory personnel who were exposed to vesicular stomatitis virus were examined for patterns of human infection and prevalence of vesicular stomatitis new jersey serotype neutralizing antibody. insight into the mechanism of transmission was sought by comparing activities of antibody-positive and antibody-negative persons. a statistically significant risk factor was a h ... | 1987 | 3028192 |
| intergenic sequences of the vesicular stomatitis virus genome (new jersey serotype): evidence for two transcription initiation sites within the l gene. | the intergenic sequences of vesicular stomatitis virus of the new jersey serotype [vsv (nj): ogden strain] have been determined by dideoxy sequencing across the gene junctions of the viral rna genome using deoxyoligonucleotide primers. the n-ns, ns-m, and m-g intergenic sequences of vsv (nj) are identical to the consensus intergenic sequence for vsv of the indiana serotype [vsv (ind)]: 3'-auacu7gauugucnnnag-5' (genome sense; n denotes any nucleotide), where 3'-auacu7-5' encodes the 3' terminus a ... | 1987 | 2820143 |
| functional analysis of hypomethylation variants of the new jersey serotype of vesicular stomatitis virus. | the ts mutant f1 of vesicular stomatitis virus, new jersey serotype, directs the synthesis of undermethylated 5'-terminal cap structures in vitro. in order to determine the relationship between the ts and hypomethylation phenotypes, a spontaneous revertant rev(ts)f1 of the ts phenotype was analyzed. the revertant retained the hypomethylation phenotype. the four cap structures (gpppa, 7mgpppa, gpppam, and 7mgpppam) synthesized in mutant and revertant-directed reactions in the presence of low as w ... | 1987 | 2821679 |
| effect of melittin on transcription by vesiculovirus mutant and wild-type viruses. | the bee venom peptide melittin activated the virion transcriptase activity of three vesiculoviruses with preservation of virion structure. the kinetics of rna synthesis were similar to those observed with purified transcribing nucleoprotein (tnp) preparations. six temperature-sensitive host range (tdce) mutants of chandipura virus displayed 1.7- to 5.5-fold greater efficiencies of transcription at 39 degrees with melittin-permeabilized virions in comparison with tnp preparations. comparative stu ... | 1987 | 2822606 |
| vesicular stomatitis virus (new jersey strain) infection in two california dairy herds: an epidemiologic study. | the course of vesicular stomatitis in cattle was investigated in 2 dairy herds (a and b) located in the southern san joaquin valley of california. cattle were examined and specimens were obtained for virus isolation and for serologic survey for one year after an epizootic in december 1982. all 33 lactating cows selected for study had oral lesions, but only 19 (58%) were drooling or frothing around the mouth. lesions on feet and teats were not observed. the healing time (longer than has been repo ... | 1987 | 2824414 |
| epizootic vesicular stomatitis in colorado, 1982: some observations on the possible role of wildlife populations in an enzootic maintenance cycle. | sera obtained from wild ungulates, carnivores, and rodents in colorado were tested for neutralizing (n) antibody against vesicular stomatitis, new jersey serotype (vsnj), virus to determine their involvement in the 1982 colorado vsnj epizootic in domestic animals. viremic and n antibody responses of two local rodent species to a 1982 colorado isolate of vsnj were determined in the laboratory. the rodents produced only weak viremias, but all developed n antibody. n antibody prevalences for vsnj i ... | 1987 | 3035241 |
| serologic survey for evidence of exposure to vesicular stomatitis virus, pseudorabies virus, brucellosis and leptospirosis in collared peccaries from arizona. | two hundred eighteen usable serum samples were collected from hunter-killed collared peccaries (tayassu tajacu) during march 1986, in three areas of arizona. evaluations for antibodies against vesicular stomatitis virus (vsv) new jersey (nj) type, vsv indiana type, pseudorabies virus, brucellosis, and leptospirosis revealed positive test results in 8%, 0%, less than 1%, 0%, and 23% of the sera, respectively. exposure of peccaries to vsv (nj) was widespread, but variation in the prevalence of ser ... | 1987 | 2824864 |