| pathogenesis of respiratory syncytial virus infection in ferret and fetal human tracheas in organ culture. | the pathogenesis of human respiratory syncytial virus infection was studied in ferret and fetal human tracheas in organ culture. although the patterns of virus growth were similar in these species, the sites and morphologic consequences of virus replication differed markedly. in human trachea, synthesis of respiratory syncytial virus occurred in a population of ciliated epithelial cells, whereas other cells in the epithelial layer were spared. virus replication was associated with cell injury ch ... | 1978 | 354444 |
| the polypeptides of human respiratory syncytial virus: products of cell-free protein synthesis and post-translational modifications. | | 1979 | 425320 |
| sequence analysis of m2 mrna of bovine respiratory syncytial virus obtained from an f-m2 dicistronic mrna suggests structural homology with that of human respiratory syncytial virus. | the nucleotide sequences of the f and m2 mrnas of strain a51908 of bovine respiratory syncytial virus (brsv) were determined by sequencing cdna of an intracellular dicistronic mrna. comparison of the f mrna sequence with those of other brsv strains showed that there was extensive sequence identity at both the nucleotide (95% identity) and amino acid (94% identity) levels. alignment of the nucleotide and encoded amino acid sequences of m2 mrna of brsv with those of human respiratory syncytial vir ... | 1992 | 1312130 |
| bovine respiratory syncytial virus fusion protein gene: sequence analysis of cdna and expression using a baculovirus vector. | the nucleotide sequence of bovine respiratory syncytial virus (rsv), atcc strain a51908 fusion (f) glycoprotein gene cdna was determined. the amino acid sequence deduced was then compared to those of two different isolates of bovine rsv, strains rb 94 and 391-2, and the a and b subtypes of human rsv, strains 18537 and a2. the bovine rsv f protein is highly conserved between the three isolates, a51908 has 97% amino acid identity to rb 94, and 99% identity to 391-2. the f proteins of both the a an ... | 1992 | 1318950 |
| [detection of respiratory syncytial and adenovirus in nasopharyngeal aspirates: comparison of cellular cultures and immunofluorescence]. | respiratory syncytial and adenovirus are 2 of the most important pathogens for respiratory infection in infancy. we compared the results of cellular cultures and immunofluorescence in the detection of these agents in nasopharyngeal exudates from hospitalized infants. evidence for respiratory syncytial virus was searched in 776 samples. immunofluorescence was much more sensitive, detecting 303 positive results, compared to only 142 for cell culture. compared to immunofluorescence, the sensitivity ... | 1992 | 1340571 |
| reshaping a human monoclonal antibody to inhibit human respiratory syncytial virus infection in vivo. | we transferred the complementarity determining regions from a murine monoclonal antibody that neutralizes infection by respiratory syncytial virus (rsv) to a human igg1 monoclonal antibody. the resulting reshaped human antibody lost affinity for rsv, but an additional alteration to one of the framework regions restored binding affinity and specificity. this second generation reshaped human monoclonal antibody cross-reacted with all clinical isolates of rsv tested and both prevented disease and c ... | 1991 | 1367535 |
| intracellular processing of the human respiratory syncytial virus fusion glycoprotein: amino acid substitutions affecting folding, transport and cleavage. | the intracellular processing and transport of the respiratory syncytial virus (rsv) fusion (f) glycoprotein was examined by comparing the maturation and stability of wild-type f, uncleaved mutant f and chimeric f glycoproteins expressed by recombinant vaccinia viruses to that of f protein expressed by rsv. one of the recombinant viruses, vf317, expressed f protein (f317) that was processed like the rsv f glycoprotein. f317 was synthesized initially as f0, the uncleaved glycosylated precursor of ... | 1992 | 1375280 |
| location of the epitope recognized by monoclonal antibody 63g on the primary structure of human respiratory syncytial virus g glycoprotein and the ability of synthetic peptides containing this epitope to induce neutralizing antibodies. | the location of the epitope recognized by monoclonal antibody (mab) 63g on the primary structure of the human respiratory syncytial virus g glycoprotein was determined by testing the reactivity of synthetic peptides with the mab. the role of individual amino acids in this epitope was determined by using a set of 13-mer peptides containing single residue deletions. residues 204 to 209 were found to be essential for antibody binding. these results are in full agreement with previous sequence data ... | 1992 | 1383397 |
| characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the fusion glycoprotein of human respiratory syncytial virus. | two antigenic sites recognized by neutralizing monoclonal antibodies (mabs) directed against the fusion (f) glycoprotein of human respiratory syncytial virus were mapped on the primary structure of the protein by (i) the identification of amino acid substitutions selected in antibody-escape mutants and (ii) the reactivity of synthetic peptides with mabs. the first site contained several overlapping epitopes which were located within the trypsin-resistant amino-terminal third of the large f1 subu ... | 1992 | 1383404 |
| sequence comparison between the phosphoprotein mrnas of human and bovine respiratory syncytial viruses identifies a divergent domain in the predicted protein. | the nucleotide and deduced amino acid sequences of the phosphoprotein (p) mrna of bovine respiratory syncytial virus (brsv) strain a51908 have been determined. the p mrna is 860 nucleotides long with a single large open reading frame and the encoded polypeptide is 241 amino acids long. comparison with the corresponding sequences of human respiratory syncytial virus (hrsv) subgroups a and b revealed 72 to 74% identity at the nucleotide level, and 81% at the amino acid level. the p protein contain ... | 1992 | 1402819 |
| transcription of human respiratory syncytial virus genome rna in vitro: requirement of cellular factor(s). | extracts made from human respiratory syncytial virus (rsv)-infected hep-2 cells synthesized mrnas encoded by all known viral genes. in contrast, rsv ribonucleoproteins purified from infected cells failed to transcribe in vitro; transcription was restored by addition of a cytoplasmic extract of uninfected hep-2 cells, demonstrating that a cellular factor(s) has a role in rsv gene expression. quantitation of the individual gene mrnas transcribed in vitro revealed polarity of transcription of the g ... | 1992 | 1404620 |
| comparison of three immunoassays for the rapid detection of bovine respiratory syncytial virus. | three enzyme-linked immunosorbent assays (eia) designed for the detection of human respiratory syncytial virus (rsv) were evaluated for the detection of bovine respiratory syncytial virus (brsv) in bovine lungs and the results were compared with those obtained by a direct fluorescent antibody assay (dfa). the eia tests used were directigen eia, kallestad pathfinder eia, and abbott rsv eia. homogenates of lung tissues obtained from 64 cattle that had died of respiratory disease were used; 32 were ... | 1992 | 1501586 |
| protective activity of a human respiratory syncytial virus immune globulin prepared from donors screened by microneutralization assay. | to explore the feasibility of preparing a human immune globulin specific for respiratory syncytial virus (rsv) by screening plasma donors, the ability of seven rsv antibody assays to identify plasma-yielding igg with high virus-neutralizing and animal-protective activities was compared. igg prepared from plasma units selected by microneutralization assay had significantly higher activity in protecting mice from respiratory rsv challenge than did iggs prepared from plasmas selected by three direc ... | 1992 | 1538152 |
| the nucleotide sequence of the l gene of marburg virus, a filovirus: homologies with paramyxoviruses and rhabdoviruses. | the nucleotide sequence of the l gene of marburg virus, strain musoke, has been determined. the l gene has a single long open reading frame encoding a polypeptide of 2330 amino acids (mw 267,175) that represents the viral rna-dependent rna polymerase. the putative transcription start signal (3'cuaccuauaauu 5') and the termination signal (3' uaauucuuuuu 5') of the gene could be identified. computer-assisted comparison of the l protein with l proteins of other nonsegmented negative-stranded rna vi ... | 1992 | 1546452 |
| identification of subgroups of bovine respiratory syncytial virus. | the occurrence of antigenic variation among nine isolates of bovine respiratory syncytial virus (brsv) was determined by examining their reaction patterns to human respiratory syncytial virus (hrsv) subgroup a and b monoclonal antibodies (mabs) by enzyme immunoassay and radioimmunoprecipitation with fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis by using mabs and polyclonal antisera to hrsv and brsv. shared epitopes were demonstrated on four of five structural protein ... | 1992 | 1583108 |
| molecular cloning and sequence analysis of the mumps virus gene encoding the l protein and the trailer sequence. | we have cloned and determined the nucleotide sequences of the seventh gene of the miyahara strain of mumps virus (muv) encoding the l protein. the l gene is 6925 nucleotides in length and contains a single long open reading frame which is capable of coding for a protein of 2261 amino acids with a calculated molecular weight of 256,571 da. the deduced amino acid sequence of the l protein of muv showed significant homology with those of six other paramyxoviruses, human parainfluenza type 2 virus, ... | 1992 | 1585659 |
| gene junction sequences of bovine respiratory syncytial virus. | the nucleotide sequences of seven gene junctions (n-p, p-m, m-sh, sh-g, g-f, f-m2 and m2-l) of bovine respiratory syncytial virus (brsv) strain a51908 were determined by dideoxynucleotide sequencing of cdnas from polytranscript mrnas and from genomic rna. by comparison with the consensus sequences derived from human respiratory syncytial virus (hrsv) mrnas, gene-start and gene-end sequences were found in all brsv mrnas. there was a perfect match between the brsv and hrsv in all gene-start sequen ... | 1992 | 1626423 |
| sequence analysis of the 22k, sh and g genes of turkey rhinotracheitis virus and their intergenic regions reveals a gene order different from that of other pneumoviruses. | the nucleotide and deduced amino acid sequences of three genes of turkey rhinotracheitis virus (trtv) together with the nucleotide sequences of the relevant intergenic regions were determined. the deduced amino acid sequence of one of the genes shows significant identity (42%) to that of the 22k protein of human respiratory syncytial virus (rsv). the trtv 22k gene, like that of rsv, has a second open reading frame, although the amino acid sequence deduced from this reading frame does not show an ... | 1992 | 1629697 |
| oligomerization and post-translational processing of glycoprotein g of human respiratory syncytial virus: altered o-glycosylation in the presence of brefeldin a. | the post-translational maturation of the attachment g glycoprotein of human respiratory syncytial virus (rsv) was investigated. the g protein formed homo-oligomers which sedimented in sucrose gradients at the same rate as the fusion f protein tetramer. oligomerization of the g protein was insensitive to carbonylcyanide m-chlorophenylhydrazine, showing that this step occurs in the endoplasmic reticulum prior to o-glycosylation which initiated in the trans-golgi compartment. the sedimentation of t ... | 1992 | 1634876 |
| purification of human respiratory syncytial virus: superiority of sucrose gradient over percoll, renografin, and metrizamide gradients. | a method was devised for producing and purifying human respiratory syncytial virus (hrsv) preparations with high titers. previous attempts to obtain substantial amounts of purified human respiratory syncytial virus have been unsuccessful due to the extreme lability of this virus, its close association with the host cell membrane, and its tendency to aggregate during concentration procedures. we describe a comparative study of various purification media as well as a novel approach for obtaining h ... | 1991 | 1650782 |
| location of a highly conserved neutralizing epitope in the f glycoprotein of human respiratory syncytial virus. | trypsin digestion of the purified f protein from human respiratory syncytial virus (long strain) generated a set of fragments in the amino-terminal third of the f1 subunit which contained the epitope 47f involved in neutralization. sequencing of five escape mutant viruses selected with monoclonal antibody 47f allowed us to map precisely two amino acid residues (262 and 268) of the f1 subunit which are essential for the integrity of this important epitope. the results are discussed in terms of th ... | 1990 | 1688629 |
| sequence comparison between the fusion protein of human and bovine respiratory syncytial viruses. | the nucleotide sequence was determined for the fusion (f) protein-coding mrna of the bovine respiratory syncytial virus (strain rb 94) and the amino acid sequence of the f protein was deduced for comparison with the sequence of human respiratory syncytial virus subtypes a and b (rss-2 and 18537 strains). the human and bovine rs virus f proteins (excluding the cleaved signal peptide) share 83 to 84% homology. the greatest divergence occurred within the f2 subunit in the region preceding the cleav ... | 1990 | 1703214 |
| expression of the f glycoprotein gene from human respiratory syncytial virus in escherichia coli: mapping of a fusion inhibiting epitope. | a cdna copy of the gene encoding the entire amino acid sequence of the fusion (f) protein of human respiratory syncytial virus (strain a2) was inserted into a bacterial expression vector containing the lambda pr promoter. upon heat induction, escherichia coli cells harboring the vector produced a 45-kda peptide which reacted with rabbit polyclonal antiserum to the native f protein. expression of the f gene resulted in severe inhibition of bacterial growth, which was overcome by deletion of the d ... | 1991 | 1714666 |
| post-translational processing and oligomerization of the fusion glycoprotein of human respiratory syncytial virus. | the post-translational maturation of the fusion protein (f) of human respiratory syncytial virus was investigated. chemical cross-linking experiments indicated that f forms homotetramers and provided evidence that the intermonomer contacts involve primarily the f1 subunit. homooligomerization as measured by sedimentation in sucrose gradients was insensitive to carbonyl cyanide m-chlorophenylhydrazone, indicating that it occurs in the endoplasmic reticulum. cleavage of the f0 precursor to yield t ... | 1991 | 1765771 |
| a chimeric glycoprotein of human respiratory syncytial virus termed fg induces t-cell mediated immunity in mice. | popliteal lymph node cells taken from mice vaccinated with the fg glycoprotein were exposed in vitro to respiratory syncytial virus (rsv) antigens. proliferation to fg or rsv antigens was blocked with anti-cd4 monoclonal antibody treatment. fg-vaccinated mice developed classical late delayed type hypersensitivity (dth) reactions when exposed to fg antigen in vivo. | 1991 | 1811371 |
| nucleotide sequences of the 3' leader and 5' trailer regions of human respiratory syncytial virus genomic rna. | the nucleotide sequences of the 3' extracistronic (leader) and 5' extracistronic (trailer) regions were determined for genomic rna (vrna) of human respiratory syncytial virus (rsv) strain a2. to sequence the 3' leader region, vrna was extracted from purified virions, size-selected, polyadenylated, copied into cdna, amplified by the polymerase chain reaction, cloned, and sequenced. the 3' leader sequence is 44 nt, which is somewhat shorter than its counterparts (50 to 70 nt) in other nonsegmented ... | 1991 | 1840712 |
| sequence of the major nucleocapsid protein gene of pneumonia virus of mice: sequence comparisons suggest structural homology between nucleocapsid proteins of pneumoviruses, paramyxoviruses, rhabdoviruses and filoviruses. | the complete nucleotide sequence of gene 3 of pneumonia virus of mice has been determined, and the 5' end of the mrna mapped using a modification of the polymerase chain reaction technique. the gene contains a single open reading frame, beginning with a 5'-proximal aug initiation codon, encoding a polypeptide with a predicted mr of 43141. expression of the gene 3 protein in escherichia coli and in vitro showed that it reacted with virus-specific antiserum and comigrated with the major nucleocaps ... | 1991 | 1848602 |
| nucleotide sequence analysis of a matrix and small hydrophobic protein dicistronic mrna of bovine respiratory syncytial virus demonstrates extensive sequence divergence of the small hydrophobic protein from that of human respiratory syncytial virus. | the nucleotide and deduced amino acid sequences of the matrix (m) and small hydrophobic (sh) proteins of bovine respiratory syncytial virus (brsv) have been determined from a dicistronic mrna. comparison of these sequences with the corresponding published sequences of human respiratory syncytial virus (hrsv) revealed extensive overall homology at both the nucleotide and amino acid levels in the m protein, but low overall homology at both the nucleotide and amino acid levels in the sh protein. th ... | 1991 | 1856698 |
| evolution of the g and p genes of human respiratory syncytial virus (subgroup a) studied by the rnase a mismatch cleavage method. | the g and p genes of human respiratory syncytial viruses (subgroup a), isolated between 1961 and 1989, were analyzed by rnase a one-dimensional fingerprinting, using the long strain as the reference. total rna extracted from cells infected with the different isolates was hybridized to radiolabeled antisense g or p rna probes of the long virus. the rna:rna heteroduplexes were digested with rnase a and the resistant products analyzed by gel electrophoresis. comparative analysis of the cleavage pat ... | 1991 | 1871967 |
| genes 1 and 2 of pneumonia virus of mice encode proteins which have little homology with the 1c and 1b proteins of human respiratory syncytial virus. | genes 1 and 2 of pneumonia virus of mice (pvm) consist of 410 and 571 nucleotides and encode proteins of 113 and 156 amino acids respectively. the proteins show no extensive (gene 1 analogous to 1c) or low (gene 2 analogous to 1b) homology to their presumed counterparts in human respiratory syncytial virus (hrsv). the strongest homology is between regions of approximately 35 amino acids located near the carboxy termini of the gene 2 product and the 1b protein with 29% identity, although a lower ... | 1991 | 1919530 |
| rescue of synthetic analogs of respiratory syncytial virus genomic rna and effect of truncations and mutations on the expression of a foreign reporter gene. | the viral genomic rna (vrna) of human respiratory syncytial virus is a nonsegmented negative strand that is not infectious alone. to develop methods for complementing synthetic vrna with viral proteins, a cdna was constructed to encode a vrna in which all of the viral protein-coding sequences were removed and replaced with a negative-sense copy of the bacterial chloramphenicol acetyltransferase gene. upon transfection into respiratory syncytial virus-infected cells, the synthetic vrna was "rescu ... | 1991 | 1946383 |
| molecular cloning and sequence analysis of bovine respiratory syncytial virus mrna encoding the major nucleocapsid protein. | the nucleotide sequence of the gene encoding the major nucleocapsid (n) protein of bovine respiratory syncytial virus (brsv) has been determined. the n mrna is 1196 nucleotides long with a single, large open reading frame. the derived polypeptide has 391 amino acids corresponding to a calculated molecular weight of 42,600 da. this is in agreement with the molecular weight of 43,000 da determined for the brsv n protein by sds-polyacrylamide gel electrophoresis (page). comparison of the nucleotide ... | 1991 | 1984667 |
| comparison of the virulence of wild-type thymidine kinase (tk)-deficient and tk+ phenotypes of vaccinia virus recombinants after intranasal inoculation of mice. | a recombinant vaccinia virus vector was constructed which expressed the major surface glycoprotein g of human respiratory syncytial virus (rsv) and the thymidine kinase (tk) gene of vaccinia virus. the virulence of this tk+ recombinant virus was compared with that of a tk- recombinant and the wild-type (wt) virus after intranasal inoculation of mice. respiratory infection with wt virus resulted in a lethal infection with widespread dissemination of virus. in contrast, infection with the tk- reco ... | 1991 | 1990060 |
| deduced amino acid sequence of the fusion glycoprotein of turkey rhinotracheitis virus has greater identity with that of human respiratory syncytial virus, a pneumovirus, than that of paramyxoviruses and morbilliviruses. | the sequence of the fusion (f) glycoprotein of turkey rhinotracheitis virus (trtv) has been deduced from cdna clones derived from oligo(dt)-selected infected cell rna. the protein consists of 538 amino acids, the f2 and f1 subunits containing 102 (including the f2-f1 connecting peptide rrrr) and 436 residues, respectively. each subunit has one potential n-linked glycosylation site. the protein has 38 to 39% amino acid identity with the f protein of respiratory syncytial virus (pneumovirus genus) ... | 1991 | 1990068 |
| antiviral effects of xanthate d609 on the human respiratory syncytial virus growth cycle. | the antiviral compound tricyclo-decan-9-yl-xanthogenate (d609) inhibits respiratory syncytial (rs) virus growth in human epithelial (hep 2) cells. d609 treatment resulted in a decrease in the accumulation of viral proteins, in the phosphorylation of the viral phosphoprotein, and in the amount of extracellular antigens and infectious particles. the relative accumulation of viral proteins was also unbalanced, however no differences were found in the amount of viral rna with plus or minus polarity. ... | 1991 | 1994569 |
| vaccination of cotton rats with a chimeric fg glycoprotein of human respiratory syncytial virus induces minimal pulmonary pathology on challenge. | the cotton rat model of human respiratory syncytial virus (rsv) infection was used to study the safety and efficacy of a chimeric fg glycoprotein that was expressed in insect cells using a baculovirus vector. histologic and virologic examination of vaccinated rat lungs was done after challenge with rsv. when rats were challenged 1 month after vaccination, severe pulmonary inflammation characterized by both a mononuclear and polymorphonuclear cell infiltrate and 30%-40% involvement of lung tissue ... | 1991 | 1995720 |
| the g protein of human respiratory syncytial virus: significance of carbohydrate side-chains and the c-terminal end to its antigenicity. | the reactivities of eighteen monoclonal antibodies with different glycosylated forms of the human respiratory syncytial (rs) virus g protein were tested in western blots. only five antibodies recognized the unglycosylated precursor. the majority of antibodies, however, reacted with the o-glycosylated form of the g protein, emphasizing the importance of this type of modification for the antigenicity of the mature molecule. human antisera, which recognized the rs virus g protein in western blots, ... | 1991 | 2005433 |
| human and bovine respiratory syncytial virus: immunopathologic mechanisms. | human respiratory syncytial virus (hrsv) is the major respiratory tract pathogen of infants and young children. bovine respiratory syncytial virus (brsv) is recognised as an important cause of respiratory tract disease in calves. both of these viruses and their respective diseases share many similarities. immunopathologic mechanisms have been proposed to be involved in the pathogenesis of respiratory syncytial virus (rsv) infections. this review examines the current understanding of the role of ... | 1991 | 2021054 |
| cytotoxic t cell activity against the 22-kda protein of human respiratory syncytial virus (rsv) is associated with a significant reduction in pulmonary rsv replication. | recombinant vaccinia viruses expressing the rsv f glycoprotein (vac-f), or a previously described chimeric protein consisting of the extracellular domains of the f and g glycoproteins (vac-fg), or the 22-kda membrane protein (vac-22 kda) were evaluated for their ability to protect balb/c mice against infection by rsv subgroup a or subgroup b viruses and for their ability to induce a humoral immune response or a cytolytic t lymphocyte (ctl) response. immunization with vac-f or vac-fg fully protec ... | 1991 | 2024493 |
| location of phosphorylated residues in human respiratory syncytial virus phosphoprotein. | the phosphoprotein (p protein) from human respiratory syncytial virus long strain, labelled in vivo with [32p]orthophosphate, was purified from virions or virus-infected human epithelial (hep-2) cells. the main phosphorylated amino acid found was serine. the determination of the n-terminal sequence of unphosphorylated and phosphorylated fragments of p protein obtained after chemical or enzymic treatments suggested that some or all of the six serines present at positions 116, 117, 119, 143, 156 a ... | 1991 | 2045795 |
| sequence analysis of the polymerase l gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses. | the complete nucleotide sequence of the large (l) polymerase gene of human respiratory syncytial virus (rsv) strain a2 was determined by analysis of cloned-cdnas representing the entire gene and confirmed in part by dideoxy sequencing of genomic rna. the rsv l gene is 6578 nucleotides in length and contains a single major open reading frame that encodes a protein of 2165 amino acids. the molecular weight (250,226) and amino acid composition of the deduced rsv l protein are similar to those of ot ... | 1991 | 2053282 |
| o glycosylation of glycoprotein g of human respiratory syncytial virus is specified within the divergent ectodomain. | cdnas encoding the g glycoprotein of respiratory syncytial virus and the hemagglutinin-neuraminidase (hn) glycoprotein of parainfluenza virus type 3 were modified by site-specific mutagenesis and restriction fragment replacement to encode chimeric proteins consisting of the cytoplasmic and transmembrane domains of one protein fused to the ectodomain of the other. in the case of the hn ectodomain attached to the g transmembrane and cytoplasmic domains, cell surface expression of the chimera was r ... | 1990 | 2164608 |
| nucleotide sequence analysis and expression from recombinant vectors demonstrate that the attachment protein g of bovine respiratory syncytial virus is distinct from that of human respiratory syncytial virus. | bovine respiratory syncytial (brs) virus causes a severe lower respiratory tract disease in calves similar to the disease in children caused by human respiratory syncytial (hrs) virus. while there is antigenic cross-reactivity among the other major viral structural proteins, the major glycoprotein, g, of brs virus and that of hrs virus are antigenically distinct. the g glycoprotein has been implicated as the attachment protein for hrs virus. we have carried out a molecular comparison of the glyc ... | 1990 | 2214024 |
| frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus. | the genetic characterization of four previously reported mutants of human respiratory syncytial (rs) virus resistant to monoclonal antibody 63g is described. sequences of the g protein genes were obtained from: (i) mrna derived cdna recombinants, (ii) direct mrna sequencing and (iii) amplified vrna derived cdnas. the results obtained indicate that the original escape mutants, recovered from individual plaques, contained heterogeneous viral populations. this heterogeneity affected the number of a ... | 1990 | 2249671 |
| the two open reading frames of the 22k mrna of human respiratory syncytial virus: sequence comparison of antigenic subgroups a and b and expression in vitro. | the sequence of the 22k mrna of strain 18537 of antigenic subgroup b of human respiratory syncytial virus (rsv) was determined by sequencing cloned cdnas of intracellular mrna. comparison with the corresponding sequence of the a2 strain of subgroup a showed that there is 78% nucleotide sequence identity overall, that the amino acid sequence of the 22k protein is 92% identical between subgroups and that the 22k mrna of both subgroups contains a second, internal, overlapping open reading frame (or ... | 1990 | 2273393 |
| analysis of genetic variability in human respiratory syncytial virus by the rnase a mismatch cleavage method: subtype divergence and heterogeneity. | we have applied the rnase a mismatch cleavage method to the analysis of genetic variability among human respiratory syncytial (rs) viruses. antisense rna probes of the long strain were hybridized to total rna extracted from cells infected with other strains. the rna:rna heteroduplexes were digested with rnase a and the resistant products analyzed by gel electrophoresis. each virus generated characteristic band patterns with the different probes. comparative analyses of the cleavage patterns indi ... | 1990 | 2294636 |
| sequence comparison of the phosphoprotein mrnas of antigenic subgroups a and b of human respiratory syncytial virus identifies a highly divergent domain in the predicted protein. | the sequences of the p mrna and protein of strain 18537 of antigenic subgroup b of human respiratory syncytial virus were determined by sequencing cloned cdnas of intracellular mrna. comparison with the corresponding sequences of the a2 strain of subgroup a showed that there was extensive sequence identity at both the nucleotide (80% identity) and amino acid (90% identity) levels. the p proteins contained a single divergent region (52% amino acid identity) flanked by highly conserved domains (96 ... | 1990 | 2307966 |
| evaluation in chimpanzees of vaccinia virus recombinants that express the surface glycoproteins of human respiratory syncytial virus. | the immunogenicity and protective efficacy of recombinant vaccinia viruses that express the two major protective antigens of human respiratory syncytial virus (rsv), the f and g glycoproteins, were evaluated in chimpanzees. in previous studies in rodents and monkeys the f and g proteins expressed by the same recombinants were highly immunogenic and induced high levels of resistance to rsv replication following subsequent challenge. in contrast, in chimpanzees, a single intradermal immunization i ... | 1990 | 2336876 |
| the small hydrophobic protein of human respiratory syncytial virus: comparison between antigenic subgroups a and b. | the nucleotide and amino acid sequences of the mrna and predicted polypeptide of the integral membrane small hydrophobic (sh) protein of human respiratory syncytial virus strain 18537 (a prototype strain of antigenic subgroup b) were determined from cloned cdna. at the nucleotide and amino acid levels there was 78% and 76% identity, respectively, with the previously described sh mrna and protein of strain a2 (a prototype strain of subgroup a). most of the amino acid substitutions occurred in the ... | 1990 | 2374008 |
| induction of a neutralizing immune response to human respiratory syncytial virus with anti-idiotypic antibodies. | anti-idiotypic (anti-id) antibodies were raised in rabbits against monoclonal antibodies that recognized either f glycoprotein 47f or g glycoprotein 63g, 62g, or 74g of the human respiratory syncytial virus long strain. anti-id sera inhibited the virus binding of the immunizing monoclonal antibodies and in some cases the binding of other antibodies reacting with overlapping epitopes. the anti-id sera also inhibited virus neutralization mediated by the original monoclonal antibodies. affinity pur ... | 1990 | 2384917 |
| two distinct subtypes of human respiratory syncytial virus. | antigenic variation of human respiratory syncytial (rs) virus strains was analysed using a collection of nine, six, six, nine and one monoclonal antibodies respectively directed against the large glycoprotein (g), fusion protein (f), matrix protein (m), nucleoprotein (np) and phosphoprotein (p) components of the long strain of rs virus. a comparison was made with seven other strains isolated during different years in radioimmune precipitation analyses and immune fluorescence tests. two different ... | 1985 | 2413163 |
| antigen mimicry involving measles virus hemagglutinin and human respiratory syncytial virus nucleoprotein. | intergenic antigenic relationships between measles virus and respiratory syncytial (rs) virus-specific structural components were studied by using monoclonal antibodies. of 75 monoclonal antibodies against these components, only one, an anti-measles virus hemagglutinin monoclonal antibody, cross-reacted. immunofluorescence analysis of measles virus- and rs virus-infected cells with this monoclonal antibody showed qualitatively different staining patterns which indicated that the antigen involved ... | 1986 | 2416956 |
| immunovirological studies on human respiratory syncytial virus structural proteins. | immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the paramyxoviridae family: the two external membrane glycoproteins h (90 000) and fo (f1, 49 000; f2, 20 000; disulfide linked), the internal membrane protein m (34 000), the nucleoprotein n (42 000), and a protein (78 000) designated p that could be the equivalent of the polymerase of the morbillivirus and paramyxovirus genus. neutralizing monoclonal ... | 1986 | 2421860 |
| human and murine cytotoxic t cells specific to respiratory syncytial virus recognize the viral nucleoprotein (n), but not the major glycoprotein (g), expressed by vaccinia virus recombinants. | the viral antigens recognized by cytotoxic t cells (ctl) have not been defined in most viruses infecting mouse or man. natural or artificial virus recombinants can be used to determine the antigen specificity of ctl directed against viruses with segmented genomes, such as influenza, but this technique is more difficult to apply to the study of unsegmented viruses. we describe here the use of recombinant vaccinia viruses, containing cdna corresponding to either the nucleoprotein (n) gene or the m ... | 1986 | 2431051 |
| gene overlap and site-specific attenuation of transcription of the viral polymerase l gene of human respiratory syncytial virus. | sequence analysis of the gene encoding polymerase l protein of respiratory syncytial virus showed that l-gene transcription initiates within its upstream neighbor, the gene encoding the 22-kda protein 22k. this is an exception to the canonical mode of sequential transcription of nonoverlapping genes described for other nonsegmented negative-strand rna viruses. as a consequence of the gene overlap, the termination/polyadenylylation signal for the 22k gene is located at nucleotides 56-68 within th ... | 1987 | 2440043 |
| the g glycoprotein of human respiratory syncytial viruses of subgroups a and b: extensive sequence divergence between antigenically related proteins. | two major antigenic subgroups (designated a and b) have been described for human respiratory syncytial virus (rsv). previously, on the basis of reactivity patterns with monoclonal antibodies, the greatest intersubgroup variation was shown to occur in the g protein, the putative attachment glycoprotein. to delineate the molecular basis for this variation, we have determined the nucleotide and deduced amino acid sequences of the g mrnas and proteins representing a subgroup a (long strain) and a su ... | 1987 | 2441388 |
| respiratory syncytial virus fusion glycoprotein: further characterization of a major epitope involved in virus neutralization. | competition experiments and biological assays with a panel of 15 monoclonal antibodies confirmed the presence of at least four antigenic sites on the fusion protein of human respiratory syncytial virus, three of which were involved in virus neutralization. one antigenic site, recognized by two strongly neutralizing antibodies, was conserved after reduction and denaturation and shown by immunoblotting to be localized on the f1 fragment of the fusion protein. cleavage of this protein with staphylo ... | 1987 | 2446731 |
| preparation and characterization of monoclonal antibodies directed against five structural components of human respiratory syncytial virus subgroup b. | mouse hybridomas producing antibodies against the structural proteins of strain wv4843, a subgroup b strain of respiratory syncytial (rs) virus, were produced by fusion of sp2/0 myeloma cells with spleen cells from balb/c mice immunized with purified preparations of the virus. after immunoprecipitation test with [35s]methionine-labelled extracellular virions, 35 clones found to produce antibodies against the fusion (f) protein, six against the member (m) protein, 21 against the nucleocapsid (np) ... | 1987 | 2447224 |
| marked differences in the antigenic structure of human respiratory syncytial virus f and g glycoproteins. | monoclonal antibodies directed against the glycoproteins of human respiratory syncytial virus were used in competitive enzyme-linked immunosorbent assays for topological mapping of epitopes. whereas epitopes of the f glycoprotein could be ascribed to five nonoverlapping antigenic sites, anti-g antibodies recognized unique epitopes, many of whose competition profiles overlapped extensively. variant viruses selected with a neutralizing (47f) anti-f antibody lost the binding for only 47f and 49f an ... | 1989 | 2463385 |
| antigenic variation of human rsv strains isolated in japan. | antigenic variations of respiratory syncytial virus (rsv) strains were analyzed using a collection of nine, seven, two, and one monoclonal antibodies (mabs), respectively, raised against the fusion protein (f), large glycoprotein (g), nucleoprotein (np), and phosphoprotein (p) components of the long strain of rsv. competitive binding assay by these mabs demonstrated eight, four, and two distinct epitopes on f, g, and np components, respectively. comparison of prototype long with ten field strain ... | 1989 | 2466102 |
| characterization of a novel human respiratory syncytial virus chimeric fg glycoprotein expressed using a baculovirus vector. | human respiratory syncytial virus (rsv) codes for two glycoproteins (f and g) which have been shown to the major targets for the host antibody response. we have expressed a novel chimeric glycoprotein (fg) in insect cells using a baculovirus vector. the chimeric glycoprotein contains the signal and extracellular regions of the rsv f glycoprotein linked to the extracellular region of the rsv g glycoprotein. beginning at the amino terminus, the chimeric glycoprotein consists of amino acids 1 to 48 ... | 1989 | 2477497 |
| detection of bovine respiratory syncytial virus using a heterologous antigen-capture enzyme immunoassay. | based on the marked antigenic similarities that exist between antigens of the human and bovine strains of respiratory syncytial virus (rsv), an enzyme immunoassay (eia) designed to detect human rsv was used to detect bovine rsv. the commercial test kit (rsv eia) consists of a solid phase (beads) coated with a capture antiserum prepared against the long strain of human rsv. the rsv eia test was compared with the method of inoculation of cell cultures and fluorescent antibody (fa) staining of lung ... | 1989 | 2488346 |
| the 1b (ns2), 1c (ns1) and n proteins of human respiratory syncytial virus (rsv) of antigenic subgroups a and b: sequence conservation and divergence within rsv genomic rna. | a 2330 nucleotide sequence spanning the 1b (ns2), ic (ns1) and n genes and intergenic regions of human respiratory syncytial virus strain 18537, representing antigenic subgroup b, was determined by sequencing cloned cdnas of intracellular mrnas. comparison with the previously reported sequences for strain a2 of subgroup a showed that 1b, 1c and n were highly conserved at the nucleotide level (78, 78 and 86% identity, respectively) and at the amino acid level (92, 87 and 96% identity, respectivel ... | 1989 | 2525176 |
| processing, surface expression, and immunogenicity of carboxy-terminally truncated mutants of g protein of human respiratory syncytial virus. | posttranslational processing and cell surface expression were examined for three c-terminally truncated mutants of the g protein of respiratory syncytial virus expressed from engineered cdnas. the truncated mutants, encoded by cdnas designated g71, g180, and g230, contained the n-terminal 71, 180, and 230 amino acids, respectively, of the 298-amino-acid g protein. to facilitate detection of g71, which reacted inefficiently with g-specific antisera, we constructed a parallel set of cdnas, designa ... | 1989 | 2535742 |
| production, characterization, and utility of monoclonal antibodies which react with a novel chimeric glycoprotein of human respiratory syncytial virus termed fg. | primary immunization of mice with recombinant vaccinia virus expressing the f or g glycoprotein of human respiratory syncytial virus followed by an intravenous boost with crude fg chimeric glycoprotein resulted in the generation of hybridomas each specific for either the f or g portion of fg. characterization of each mab was determined following binding to various viral and glycoprotein antigens, by immunoprecipitation, by competition binding and by subclass determination. relative affinity was ... | 1989 | 2613268 |
| immunization of cotton rats with the human respiratory syncytial virus f glycoprotein produced using a baculovirus vector. | the f glycoprotein of respiratory syncytial virus in insect cells was produced using a baculovirus expression vector to examine its potential as a subunit vaccine. two different forms of the f glycoprotein were expressed: the intact f (f) glycoprotein and the truncated (ft) glycoprotein, in which the cooh-terminal anchor region was deleted. the f glycoprotein remained cell associated, whereas the ft glycoprotein was secreted into the media of infected cells. in contrast to the processing of the ... | 1989 | 2644369 |
| protection of cotton rats against human respiratory syncytial virus by vaccination with a novel chimeric fg glycoprotein. | the cotton rat model of experimental human respiratory syncytial virus (rsv) infection was used to study the efficacy of fg, a novel chimeric glycoprotein which was expressed in insect cells using a baculovirus vector. fg contained the extracellular regions of the f (fusion) and g (attachment) glycoproteins of rsv. vaccination with fg resulted in induction of neutralizing antibody and was correlated with protection of lung tissue from rsv challenge against both serogroup a and b virus strains. b ... | 1989 | 2677234 |
| structure and cell surface maturation of the attachment glycoprotein of human respiratory syncytial virus in a cell line deficient in o glycosylation. | the synthesis of the extensively o-glycosylated attachment protein, g, of human respiratory syncytial virus and its expression on the cell surface were examined in a mutant chinese hamster ovary (cho) cell line, ldld, which has a defect in protein o glycosylation. these cells, used in conjunction with an inhibitor of n-linked oligosaccharide synthesis, can be used to establish conditions in which no carbohydrate addition occurs or in which either n-linked or o-linked carbohydrate addition occurs ... | 1989 | 2677404 |
| interprotein disulfide bonding between f and g glycoproteins of human respiratory syncytial virus. | the envelope glycoprotein g, of human respiratory virus was purified by immunoaffinity chromatography using a monoclonal antibody reacting with g glycoprotein. the purified material was analyzed for its protein patterns and by western blot for its reactivity with specific monoclonal antibodies. in addition to the g specific proteins at 90 and 55 kilodalton (kda) range, high molecular weight species were coeluted with g protein. three high molecular weight species were noticed: one (140 kda) reac ... | 1989 | 2719556 |
| fatty acid acylation of the fusion glycoprotein of human respiratory syncytial virus. | we describe the covalent attachment of palmitate to the fusion glycoprotein of respiratory syncytial virus and the identification of the attachment site. labeling of respiratory syncytial virus-infected vero cells with [3h]palmitate, followed by the purification and subsequent analysis of the fusion glycoprotein in conjunction with polyacrylamide gel electrophoresis, demonstrated that the fatty acid is covalently attached to the f1 subunit of the fusion glycoprotein. the bound palmitate was sens ... | 1989 | 2732224 |
| polypeptides of pneumonia virus of mice. i. immunological cross-reactions and post-translational modifications. | a murine polyclonal antiserum and monoclonal antibodies have been employed to identify pneumonia virus of mice (pvm) polypeptides in infected cells and to study post-translational modifications. immunoprecipitation experiments using a murine polyclonal antiserum and a monoclonal antibody directed against a 39k protein have established an antigenic relationship between two pvm proteins and the n and p proteins of human respiratory syncytial virus. although 20 virus-specific polypeptides have been ... | 1989 | 2732719 |
| participation of cytoskeletal intermediate filaments in the infectious cycle of human respiratory syncytial virus (rsv). | rsv infection of hep-2 or hela cells leads to biochemical and morphological changes of cytoskeletal intermediate filaments (if). thus, human cytokeratin 18 is modified to generate a more acidic polypeptide of slightly larger apparent molecular weight. in addition, the amounts of vimentin and other cytokeratins are reduced, probably as a consequence of proteolytic degradation. these changes are reflected in a decrease of immunofluorescence with specific antibodies in rsv-induced syncytia and a mo ... | 1988 | 2837016 |
| nucleotide sequences of the 1b and 1c nonstructural protein mrnas of human respiratory syncytial virus. | the genes encoding the 1c and 1b mrnas of human respiratory syncytial (rs) virus are first in the order of viral transcription and encode nonstructural (ns) proteins of approximate molecular weights 14,000 and 11,000, respectively, estimated by gel electrophoresis. the complete nucleotide sequences of the 1c and 1b mrnas determined from several full-length cdna clones are described. the 1c and 1b mrnas contain 528 and 499 nucleotides, respectively, exclusive of poly(a), and encode proteins of 13 ... | 1985 | 2998021 |
| nucleotide sequence of the gene encoding the fusion glycoprotein of newcastle disease virus. | the nucleotide sequence of the gene encoding the fusion (f) glycoprotein of the beaudette c strain of newcastle disease virus (ndv) has been determined from cdna clones obtained from virion rna. the gene is 1792 nucleotides long, including mrna start and polyadenylation signals typical of paramyxoviruses. the single open reading frame encodes a polypeptide of 553 amino acids, with a predicted molecular weight of 59042. the f polypeptide has three regions of high hydrophobicity: an n-terminal sig ... | 1986 | 3025345 |
| evaluation in non-human primates of the safety, immunogenicity and efficacy of recombinant vaccinia viruses expressing the f or g glycoprotein of respiratory syncytial virus. | it has been shown previously that immunization with recombinant vaccinia viruses expressing the f or g envelope glycoprotein of human respiratory syncytial virus (rsv) strain a2 induced a protective immune response in the lower respiratory tract of cotton rats against live rsv challenge. as a continuation of these studies, the safety, immunogenicity and efficacy of these recombinant vaccinia viruses was evaluated in non-human primates. rhesus and patas monkeys were each inoculated intradermally ... | 1988 | 3072795 |
| the fusion glycoproteins of human respiratory syncytial virus of subgroups a and b: sequence conservation provides a structural basis for antigenic relatedness. | two major antigenic subgroups (a and b) have been described for human respiratory syncytial virus. the complete nucleotide sequence was determined for the fusion (f) mrna of the subgroup b strain 18537 and the amino acid sequence of the f protein deduced, for comparison with the previously described sequences for the a2 strain of subgroup a. the f proteins (excluding the cleaved signal peptide) were 91% identical between the subgroups, consistent with the previously described high degree of anti ... | 1988 | 3171553 |
| the a and b subgroups of human respiratory syncytial virus: comparison of intergenic and gene-overlap sequences. | intergenic and flanking gene regions for the 1c-1b, 1b-n, n-p, m-1a, g-f and f-22k gene junctions of respiratory syncytial virus strain 18537, representing antigenic subgroup b, were determined by dideoxynucleotide sequencing of polycistronic cdnas and mrnas. comparison with their counterparts from the subgroup a strain a2 showed that the intergenic sequences were not conserved within or between the strains. flanking non-coding gene sequences also were generally not conserved except for the high ... | 1988 | 3183631 |
| immunological differences between the envelope glycoproteins of two strains of human respiratory syncytial virus. | the envelope glycoproteins of two distinct strains of respiratory syncytial virus (rsv) (long and 18537 strains) were purified by affinity chromatography and characterized by immunological methods. the fusion (f) proteins from the two strains were similar in molecular weight by gel electrophoresis and were very closely related immunologically. rabbit antisera to either f protein reacted with near equivalent titres with the heterologous f protein by western blot, enzyme-linked immunoassay (eia), ... | 1987 | 3302101 |
| antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups a and b: evaluation of the contributions of f and g glycoproteins to immunity. | the degree of antigenic relatedness between human respiratory syncytial virus (rsv) subgroups a and b was estimated from antibody responses induced in cotton rats by respiratory tract infection with rsv. glycoprotein-specific enzyme-linked immunosorbent assays of antibody responses induced by rsv infection demonstrated that the f glycoproteins of subgroups a and b were antigenically closely related (relatedness, r approximately 50%), whereas the g glycoproteins were only distantly related (r app ... | 1987 | 3305988 |
| immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. | previous reports have established that vaccinia virus (vv) recombinants expressing g, f, or n protein of respiratory syncytial (rs) virus protect small animals against intranasal challenge with live rs virus. this work demonstrates that a variety of parameters affect the protection induced by recombinant viruses. the route of vaccination, the subtype of challenge virus, and the species used influenced the antibody titers and extent of protection. during these studies, observations were also made ... | 1987 | 3316707 |
| adenosine deaminase inhibitors. synthesis and biological activity of deaza analogues of erythro-9-(2-hydroxy-3-nonyl)adenine. | two new deaza analogues of erythro-9-(2-hydroxy-3-nonyl)adenine (ehna, 1), 7-deaza-ehna (6) and 1,3-dideaza-ehna (11), were synthesized and evaluated for adenosine deaminase (ada) inhibitory activity and compared with ehna, 1-deaza-ehna (2), and 3-deaza-ehna (3). substitution of a methine group for a nitrogen atom in the 7-position of the purine moiety of ehna produces a dramatic drop in the inhibitory activity (ki = 4 x 10(-4) m) whereas compounds 2 and 3 are still good inhibitors (ki = 1.2 x 1 ... | 1988 | 3339608 |
| strain-specific serum antibody responses in infants undergoing primary infection with respiratory syncytial virus. | we sought to characterize the human respiratory syncytial virus (rsv) subgroup-specific antibody response to primary infection with rsv. rsv isolates from 43 infants and young children were typed as either subgroup a (long strain-like) or b (18537 strain-like) based on reactivity with monoclonal antibodies. acute-phase or preinfection and convalescent-phase sera were collected from the 43 subjects and tested in an enzyme-linked immunosorbent assay using purified f and g glycoproteins from rsv su ... | 1988 | 3346563 |
| electron microscopic evidence for bridges between bovine respiratory syncytial virus particles. | electron microscopic examination of ultrathin sections of a continuous cell line of ovine kidney (ok) origin, infected by bovine respiratory syncytial virus (brsv), revealed the presence of well defined bridges between virus particles. this is the first report of this novel structure. observation of ultrathin sections of human rsv long strain also grown on ok cells did not show inter-particle bridges and therefore suggested that this structure could be specific to brsv. the biological significan ... | 1988 | 3385409 |
| expression of the major glycoprotein g of human respiratory syncytial virus from recombinant vaccinia virus vectors. | the major glycoprotein, g, of human respiratory syncytial (rs) virus is a mr 84,000-90,000 species that has about 60% of its mass contributed by carbohydrate, most of which is in the form of o-linked oligosaccharides. the g protein contains neither a hydrophobic n-terminal signal sequence nor a hydrophobic c-terminal anchor region. instead, its amino acid sequence reveals only one region with significant hydrophobic character, which is between residues 38 and 66. in order to study the synthesis, ... | 1986 | 3455762 |
| nucleotide sequences for the gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. | complete sequences for the intergenic regions of the genome of human respiratory syncytial virus were obtained by dideoxynucleotide sequencing using synthetic oligonucleotides. these experiments established that the 10 respiratory syncytial viral genes are arranged, without additional intervening genes, in the order 3' 1c-1b-n-p-m-1a-g-f-22k-l 5'. for the first nine genes, the exact gene boundaries were identified by comparison of the genomic sequences with previously determined mrna sequences. ... | 1986 | 3460060 |
| in vitro and in vivo stimulation of murine lymphocytes by human respiratory syncytial virus strains. | respiratory syncytial virus (rsv) strains of subtype a (a2, wv9894, and wv12138) and of subtype b (wv1293, wv4843, and wv6873) are mitogenic in vitro for unprimed balb/c spleen cells. the virus also triggered splenocytes in vitro to secrete immunoglobulins. plaque-purified and uv-irradiated materials of both rsv subtypes produced comparable levels of dna synthesis. infectious materials of both subtypes also induced pronounced responses. lymphocyte activation with uv-inactivated rsv strain a2 was ... | 1987 | 3498209 |
| resistance to human respiratory syncytial virus (rsv) infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the rsv g glycoprotein. | a cdna copy of the g glycoprotein gene of human respiratory syncytial virus (rsv) was placed under control of a vaccinia virus promoter and inserted into the thymidine kinase locus of the vaccinia virus genome. the recombinant vaccinia virus retained infectivity and expressed a 93-kda protein that migrated with the authentic rsv g glycoprotein upon polyacrylamide gel electrophoresis. glycosylation of the expressed protein and transport to the cell surface were demonstrated in the absence of othe ... | 1986 | 3513191 |
| antigenic variation between human respiratory syncytial virus isolates. | three hybridoma antibodies, prepared against the rsn-2 strain of human respiratory syncytial (rs) virus, have been used to identify antigenic variation between 41 isolates of rs virus collected from widely separated geographical regions over a period of 29 years. one antibody was directed against an antigenic site on the virus fusion protein, vp70. this site was shared by 21 virus isolates tested and its recognition by the antibody was sensitive to the presence of 2-mercaptoethanol. the remainin ... | 1986 | 3517224 |
| expression of the f glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the f and g glycoproteins to host immunity. | a cdna clone representing the mrna coding sequence of the fusion glycoprotein (f) gene of human respiratory syncytial virus (rsv) was constructed and inserted into the thymidine kinase gene of vaccinia virus (wr strain) under the control of a vaccinia virus promoter. the resulting recombinant vaccinia virus, vaccinia f, expressed the f1 and f2 cleavage products (48 and 20 kda, respectively) of the f glycoprotein in cell culture. f1 and f2 were indistinguishable from their authentic rsv counterpa ... | 1986 | 3532115 |
| recombinant vaccinia viruses carrying the n gene of human respiratory syncytial virus: studies of gene expression in cell culture and immune response in mice. | the construction and characterization of vaccinia virus recombinants carrying the nucleocapsid (n) protein gene of human respiratory syncytial (rs) virus are described. recombinant viruses were constructed that contained the n gene oriented either positively or negatively with respect to the 7.5-kilodalton vaccinia virus promoter. in addition, a positively oriented recombinant was constructed that lacked an out-of-frame aug codon in the 5'-terminal noncoding region. in hep-2 cells, both positive ... | 1987 | 3612955 |
| human respiratory syncytial virus glycoprotein g expressed from a recombinant vaccinia virus vector protects mice against live-virus challenge. | recombinant vaccinia virus vectors were constructed which expressed the major surface glycoprotein g of human respiratory syncytial (rs) virus. the biological activity of the g protein expressed from these vectors was assayed. inoculation of rabbits with live recombinant virus induced high titers of antibody which specifically immunoprecipitated rs virus g protein and was capable of neutralizing rs virus infectivity. immunization of mice by either the intranasal or the intraperitoneal route with ... | 1986 | 3773052 |
| expression of the fusion protein of human respiratory syncytial virus from recombinant vaccinia virus vectors and protection of vaccinated mice. | vaccinia virus (vv) recombinants were constructed that contained full-length cdna copies of the fusion (f) protein gene of human respiratory syncytial (rs) virus. the f protein gene was placed next to the strong early-late vv 7.5-kilodalton promoter and was located within the vv thymidine kinase (tk) gene. full-length recombinant transcripts that initiated at both the tk and the 7.5-kilodalton promoters accumulated in cells early in infection, and one or more of these transcripts was translated ... | 1987 | 3806789 |
| the envelope-associated 22k protein of human respiratory syncytial virus: nucleotide sequence of the mrna and a related polytranscript. | we recently determined that respiratory syncytial virus (strain a2) encodes a fourth unique envelope-associated virion protein that has molecular weight of approximately 24,000, as estimated by gel electrophoresis. the nucleotide sequence of the mrna encoding this novel protein has now been determined from five cdna clones, including three that contain the complete mrna sequence. the complete mrna sequence is 957 nucleotides, exclusive of polyadenylate, and contains two partially overlapping ope ... | 1985 | 3838351 |
| nucleotide sequence of the g protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. | the major surface glycoprotein (g) of human respiratory syncytial (rs) virus has an estimated mature mr of 84,000-90,000. among a library of cdna clones prepared from rs virus mrnas, we identified clones that hybridized to a message that encoded a mr 36,000 polypeptide that was specifically immunoprecipitated with anti-g antiserum. the amino acid sequence of the g protein backbone was determined by nucleotide sequence analysis of several of the cdna clones. it contains a combination of structura ... | 1985 | 3858865 |
| the 1a protein gene of human respiratory syncytial virus: nucleotide sequence of the mrna and a related polycistronic transcript. | the 1a mrna is the smallest mrna of human respiratory syncytial (rs) virus and encodes a single protein of approximate molecular weight 9500 as estimated previously by gel electrophoresis. the nucleotide sequence of the 1a mrna, determined from several full-length cdna clones, is reported. the 1a mrna consists of 405 nucleotides, exclusive of poly(a), with relatively long nontranslated regions at the 5' and 3' ends (84 and 126 nucleotides, respectively). the sequences at the 5' and 3' termini of ... | 1985 | 3879976 |
| characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. | a total of 13 respiratory syncytial (rs) virus specific polypeptides were identified by pulse-chase metabolic labeling of infected hep-2 cells. ten of the 13 proteins were shown to be unique. they were the l, g, f (f1, f2), n, p, m, 24k, 14k, 11k and 9.5k proteins. these conclusions were based on peptide mapping and on previous work showing that each of 10 polypeptides are coded for by a unique mrna. the seven largest proteins, l, g, f (f1, f2), n, p, m and 24k were identified clearly as virion ... | 1985 | 3993233 |
| mrna sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22k structural protein. | an mrna sequence of two human respiratory syncytial viral nonstructural protein genes and of a gene for a 22,000-molecular-weight (22k) protein was obtained by cdna cloning and dna sequencing. sequences corresponding to the 5' ends of the respective transcripts were deduced directly by primer extension and dideoxy nucleotide sequencing of the mrnas. the availability of a bicistronic clone (prsc6) confirmed the gene order for this portion of the genome. contrary to other unsegmented negative-stra ... | 1985 | 4009789 |
| respiratory syncytial virus envelope glycoprotein (g) has a novel structure. | amino acid sequence of human respiratory syncytial virus envelope glycoprotein (g) was deduced from the dna sequence of a recombinant plasmid and confirmed by limited amino acid microsequencing of purified 90k g protein. the calculated molecular mass of the protein encoded by the only long open reading frame of 298 amino acids was 32,588 daltons and was somewhat smaller than the 36k polypeptide translated in vitro from mrna selected by this plasmid. inspection of the sequence revealed a single h ... | 1985 | 4069997 |
| nucleotide sequence of the gene encoding the fusion (f) glycoprotein of human respiratory syncytial virus. | the nucleotide sequence of the mrna encoding the f protein of respiratory syncytial (rs) virus (strain a2) was determined from cdna clones that contain the complete mrna sequence. the mrna is 1899 nucleotides long exclusive of polyadenylylate. the single major open reading frame encodes a protein of 574 amino acids, with a calculated molecular weight of 63,453. major structural features predicted from the amino acid sequence include an nh2-terminal signal sequence (residues 1-22), hydrophobic tr ... | 1984 | 6096849 |
| cdna cloning and transcriptional mapping of nine polyadenylylated rnas encoded by the genome of human respiratory syncytial virus. | we have isolated cdna clones representing nine unique poly(a)+ rnas transcribed from the genome of human respiratory syncytial virus, a paramyxovirus. a cdna library was constructed by using poly(a)+ rna from virus-infected cells as template and the escherichia coli plasmid pbr322 as vector. viral cdna clones were identified by hybridization with cdna probes prepared from viral genomic rna. the viral clones were grouped into nine different families by hybridization with individual size-selected ... | 1983 | 6190173 |