| sequence characterization of the matrix protein genes of parainfluenza virus types 4a and 4b. | the complete nucleotide sequences of the matrix protein (m) genes of parainfluenza virus types 4a and 4b (piv-4a and -4b) were determined from cdna of the mrna, and found to be 1548 bases in length, exclusive of poly(a) sequences. the sequences contained a large open reading frame of 1146 nucleotides encoding 362 amino acids. a high degree of identity (96.1%) was observed between the amino acid sequences of piv-4a and piv-4b m. these m sequences were compared with those of 10 other paramyxovirus ... | 1991 | 1654379 |
| strain variation in parainfluenza virus type 4. | variations in epitopes on structural proteins of four isolates of parainfluenza virus type 4 (piv-4) and the mr of polypeptides in these isolates were determined by radioimmune precipitation assay with monoclonal antibodies to parainfluenza virus type 4a (piv-4a) and type 4b (piv-4b). three isolates antigenically resembled the prototype piv-4a and the sizes of their structural proteins were 72k (hn protein), 61k (f0 protein), 61k (np protein) and 40k (m protein). however, one virus isolate showe ... | 1990 | 1695674 |
| sequencing analyses and comparison of parainfluenza virus type 4a and 4b np protein genes. | the nucleotide sequences of the cdna copies of the mrna coding for the nucleocapsid proteins (nps) of human parainfluenza viruses type 4a (piv-4a) and type 4b (piv-4b) were determined. the copy of piv-4a np mrna contained 1885 nucleotides encoding a protein with a calculated molecular weight of 62,561. the same number of amino acids with a similar molecular weight (62,425) were predicted for the piv-4b np protein. comparisons on the nucleotide sequence and the amino acid sequence of np protein b ... | 1990 | 2152988 |
| sequence analysis of the phosphoprotein (p) genes of human parainfluenza type 4a and 4b viruses and rna editing at transcript of the p genes: the number of g residues added is imprecise. | we cloned and sequenced the cdnas against genomic rnas and mrnas for phosphoproteins (ps) of human parainfluenza virus types 4a (piv-4a) and 4b (piv-4b). the piv-4a and -4b p genes were 1535 nucleotides including poly(a) tract and were found to have two small open reading frames, neither of which was apparently large enough to encode the p protein. a cluster of g residues was found in genomic rna and the number of g residues was 6 in both piv-4a and -4b. however, the number of g residues at the ... | 1990 | 2167560 |
| isolation and characterization of monoclonal antibodies to human parainfluenza virus type 4 and their use in revealing antigenic relation between subtypes 4a and 4b. | eighty monoclonal antibodies (mabs) against parainfluenza virus type 4(piv-4) were isolated and characterized. of 50 mabs against piv-4a, 14 reacted with the nucleocapsid (np) protein, 11 with the hemagglutinin-neuraminidase (hn) glycoprotein, 6 with the fusion (f) glycoprotein, and 19 with the matrix (m) protein. with the aid of the piv-4a and piv-2 specific mabs showing cross-reactivity with piv-4b, the structural proteins of piv-4b were identified. gp72, p65, gp65, gp55, p53, and p40 of piv-4 ... | 1989 | 2472703 |
| [isolation of parainfluenza virus type 4b in kiev]. | | 1974 | 4374824 |
| n-glycosylation contributes to the limited cross-reactivity between hemagglutinin neuraminidase proteins of human parainfluenza virus type 4a and 4b. | cdnas encoding human parainfluenza virus type 4b (hpiv-4b) hemagglutinin neuraminidase (hn) protein were cloned and the nucleotide sequences were determined. a high degree of identity (81.4%) was observed between the nucleotide sequences of hpiv-4a and -4b hn proteins, and an 87.3% identity was found between the deduced amino acid sequences. this degree of identity is considered to be greater than immunological similarity between hpiv-4a and -4b hn proteins determined using monoclonal antibodies ... | 2000 | 11034551 |
| comparative analysis of the full-length genome sequence of a clinical isolate of human parainfluenza virus 4b. | we are engaged in airborne transmission and epidemiology studies of respiratory pathogens, with particular interest in human parainfluenza virus type 4 (hpiv-4) and other lesser studied viruses. in this paper, hpiv-4 was detected in primary rhesus monkey kidney (prmk) cells that had been inoculated with nasopharyngeal swab material obtained from a child with a mild upper respiratory tract illness. attempts to isolate the virus in pure culture were hampered by the presence of a fast-growing simia ... | 2012 | 24278751 |