| antigenic relationship of the feline infections peritonitis virus to coronaviruses of other species. | utilizing the direct and indirect fluorescent antibody procedure, the antigenic relationship of the feline infectious peritonitis virus (fipv) to 7 other human and animal coronaviruses was studied. fipv was found to be closely related to transmissible gastroenteritis virus (tgev) of swine. transmissible gastroenteritis virus and fipv were in turn antigenically related to human coronavirus 229e (hcv-229e) and canine coronavirus (ccv). an interesting finding in the study was that the 8 coronavirus ... | 1978 | 81044 |
| brief communication: replication of feline infectious peritonitis virus in organ cultures of feline tissue. | | 1978 | 208815 |
| feline infectious peritonitis virus. ii. propagation in suckling mouse brain. | | 1978 | 211754 |
| seroepidemiology of feline infectious peritonitis virus infections using transmissible gastroenteritis virus as antigen. | | 1977 | 341605 |
| feline infectious peritonitis virus. | | 1977 | 878736 |
| morphologic and physical characteristics of feline infectious peritonitis virus and its growth in autochthonous peritoneal cell cultures. | characteristic viral-type particles were seen in liver of kittens experimentally infected with the feline infectious peritonitis (fip) agent. the particles were from 70 to 75 nm in diameter, with a central doughnut-shaped nucleoid 50 to 55 nm in diameter; numerous spikelike projections extended from their envelopes. similar particles were seen by electron microscopy in peritoneal cell cultures derived from the peritoneal exudate of experimentally infected kittens, and viral antigens were identif ... | 1976 | 1275343 |
| monoclonal antibodies to the spike protein of feline infectious peritonitis virus mediate antibody-dependent enhancement of infection of feline macrophages. | antibody-dependent enhancement of virus infection is a process whereby virus-antibody complexes initiate infection of cells via fc receptor-mediated endocytosis. we sought to investigate antibody-dependent enhancement of feline infectious peritonitis virus infection of primary feline peritoneal macrophages in vitro. enhancement of infection was assessed, after indirect immunofluorescent-antibody labelling of infected cells, by determining the ratio between the number of cells infected in the pre ... | 1992 | 1309922 |
| a study of naturally occurring feline coronavirus infections in kittens. | feline coronavirus is a common infection in cats, as indicated by the high prevalence of antibodies against the virus, especially in multicat households. approximately 5 to 12 per cent of seropositive cats develop classical feline infectious peritonitis. a survey of kittens born into households of seropositive cats demonstrated the existence of healthy coronavirus carriers. seronegative animals did not appear to excrete virus. no specific antibody titre could be linked to carrier status and some ... | 1992 | 1313617 |
| immunization of cats against feline infectious peritonitis with anti-idiotypic antibodies. | anti-idiotypic antibodies (ab2s) generated against neutralizing antibodies (ab1s) specific for feline infectious peritonitis virus (fipv) were shown to be specific for paratope-associated idiotopes of the ab1s and not against isotypic determinants. in a study to determine the efficacy of an anti-idiotypic vaccine against feline infectious peritonitis (fip), cats that were immunized with a pool of monoclonal ab2s developed ab3s that recognized the variable regions of the ab2s as well as the natur ... | 1992 | 1319173 |
| comparison of early and late feline immunodeficiency virus encephalopathies. | the study of the early and late stages of encephalopathy following infection by the feline immunodeficiency virus (fiv) was carried out with laboratory and naturally infected cats. | 1992 | 1319717 |
| a novel glycoprotein of feline infectious peritonitis coronavirus contains a kdel-like endoplasmic reticulum retention signal. | a new protein of feline infectious peritonitis coronavirus (fipv) was discovered in lysates of [35s]cysteine-labeled infected cells. expression of open reading frame (orf) 6b of fipv in recombinant vaccinia virus-infected cells was used to identify it as the 6b protein. further characterization revealed that it is a novel type of viral glycoprotein whose function is not clear. it is a soluble protein contained in microsomes; its slow export from the cell is caused by the presence of an endoplasm ... | 1992 | 1321279 |
| humoral immune responses of cats to feline infectious peritonitis virus infection. | immunoperoxidase antibody (ipa) method as a titrating method of feline infectious peritonitis (fip) virus (fipv) was developed for titrating antibody to fipv (ipa-titer). by this method the immune responses of the cats that had been infected with fipv, were traced. the infected cats could be grouped into three types by their immune response to fipv and clinical appearances. type i cats lived for a long time, formed a major group among infected cats, had 160 to 1 x 10(4) ipa-titers, and showed he ... | 1992 | 1322714 |
| the prevalence of types i and ii feline coronavirus infections in cats. | the types of feline coronaviruses that are prevalent throughout japan were determined by competitive enzyme-linked immunosorbent assay (elisa) using a monoclonal antibody (mab) to feline infectious peritonitis virus (fipv) type ii and neutralizing test using type ii fipv as challenge virus. a total of 1,079 cat serum samples were tested by indirect fluorescent antibody (ifa) assay for fipv type ii antigen, all 42 sample from natural cases of fip, 138 of 647 (21.3%) from cases with some chronic d ... | 1992 | 1322718 |
| genomic organization and expression of the 3' end of the canine and feline enteric coronaviruses. | the genomic organization at the 3' end of canine coronavirus (ccv) and feline enteric coronavirus (fecv) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (fipv) and transmissible gastroenteritis virus (tgev) of swine. comparison of the latter two has previously revealed an extra open reading frame (orf) at the 3' end of the fipv genome, lacking in tgev, which is currently designated orf 6b. both ccv and fecv possess 6b-related orfs at the 3' ends of ... | 1992 | 1329312 |
| induction and enhancement of feline infectious peritonitis by canine coronavirus. | preexisting antibody to feline infectious peritonitis virus (fipv) causes acceleration and enhancement of disease on subsequent infection of cats with fipv. other workers have shown that canine coronavirus (ccv) can infect cats subclinically, but have found no evidence of enhancement of, or protection against, subsequent fipv infection. with various isolates of ccv, we determined that 1 strain of ccv can induce transient mild diarrhea in cats and, furthermore, that previous infection with ccv ca ... | 1992 | 1329586 |
| differential in vitro inhibition of feline enteric coronavirus and feline infectious peritonitis virus by actinomycin d. | the growth of feline enteric coronavirus strain 79-1683 in whole feline embryo cells was inhibited by the presence of 1 microgram/ml of actinomycin d in the culture fluid. no virus-specific mrnas could be detected in such cultures and yields of infectious virus were depressed by > 99%. by contrast, the antigenically related feline infectious peritonitis virus strain 79-1146 was unaffected by the presence of actinomycin d, indicating a fundamental difference between the two feline coronavirus str ... | 1992 | 1335030 |
| antigenic variation among transmissible gastroenteritis virus (tgev) and porcine respiratory coronavirus strains detected with monoclonal antibodies to the s protein of tgev. | five nonneutralizing monoclonal antibodies (mab) generated to the virulent miller strain of transmissible gastroenteritis virus (tgev) and specific for the s protein were characterized. competition assays between purified and biotinylated mab indicated that mab 75b10 and 8g11 mapped near a new subsite, designated v and 2 mab, 44c11 and 45a8, mapped to a previously designated subsite d. a fifth mab mapped between subsites v and e. these mab were tested with 3 previously characterized mab to subsi ... | 1992 | 1379786 |
| monoclonal antibody analysis of neutralization and antibody-dependent enhancement of feline infectious peritonitis virus. | fifty-four monoclonal antibodies (mabs) to feline infectious peritonitis virus (fipv) were characterized according to protein specificity, immunoglobulin subclass, virus neutralization, reactivity with different coronaviruses, and ability to induce antibody-dependent enhancement (ade) of fipv infection in vitro. the mabs were found to be specific for one of three structural proteins of fipv. a total of 47 mabs were specific for the 205-kda spike protein (s), 3 mabs were specific for the 45-kda n ... | 1992 | 1383568 |
| analysis of a 9.6 kb sequence from the 3' end of canine coronavirus genomic rna. | we have analysed the organization of the 3' end of the genomic rna of canine coronavirus (ccv), a virus which has a close antigenic relationship to transmissible gastroenteritis virus (tgev), porcine respiratory coronavirus (prcv) and feline infectious peritonitis virus (fipv). genomic rna isolated from ccv strain insavc-1-infected a72 cells was used to generate a cdna library. overlapping clones, spanning approximately 9.6 kb [from the 3' end of the polymerase gene, 1b, to the poly(a) tail] wer ... | 1992 | 1431811 |
| [the effectiveness of paramunization for the control of feline coryza]. | 20 cats in a cat home were treated prophylactically and therapeutically with baypamun hk. the animals were allocated into three groups as described. 7 freshly admitted clinically healthy cats were treated prophylactically on day 1, 2 and 9 with 1 ml baypamun hk (group i). 7 cats, who already were allocated for one year in the home and were sick of the feline respiratory disease complex were treated as described for group i (group ii). 6 further cats, who also showed symptoms of the feline respir ... | 1992 | 1524577 |
| differentiation between transmissible gastroenteritis virus and porcine respiratory coronavirus using a cdna probe. | a plasmid, pg3bs, containing a cdna clone from the 5' coding region of the peplomer glycoprotein gene appears to be specific for enteric transmissible gastroenteritis virus (tgev) strains and for live-attenuated tgev vaccines. this cdna probe is used to differentiate porcine respiratory coronavirus (prcv) isolates from tgev field and vaccine strains by a slot blot hybridization assay. probe pg3bs also hybridizes to canine coronavirus (ccv) rna but does not hybridize to antigenically related feli ... | 1991 | 1645595 |
| characterization of a feline infectious peritonitis virus isolate. | | 1991 | 1650605 |
| antigenic analysis of feline coronaviruses with monoclonal antibodies (mabs): preparation of mabs which discriminate between fipv strain 79-1146 and fecv strain 79-1683. | we prepared 31 monoclonal antibodies (mabs) against either fipv strain 79-1146 or fecv strain 79-1683, and tested them for reactivity with various coronaviruses by indirect fluorescent antibody assay (ifa). sixteen mabs which reacted with all of the 11 strains of feline coronaviruses, also reacted with canine coronavirus (ccv) and transmissible gastroenteritis virus (tgev). in many of them, the polypeptide specificity was the recognition of transmembrane (e1) protein of the virus. we succeeded i ... | 1991 | 1653482 |
| perspectives on the epizootiology of feline enteric coronavirus and the pathogenesis of feline infectious peritonitis. | this review presents some current thoughts regarding the epizootiology of the feline coronaviruses; feline infectious peritonitis virus (fipv) and feline coronavirus (fecv) with primary emphasis on the pathogenesis of these viruses in nature. although the mechanism(s) whereby fipv causes disease are still incompletely understood, there have been significant contributions to the literature over the past decade which provide a framework upon which plausible explanations can be postulated. two conc ... | 1991 | 1654668 |
| a study on the mechanism of antibody-dependent enhancement of feline infectious peritonitis virus infection in feline macrophages by monoclonal antibodies. | enhancement of feline infectious peritonitis virus (fipv) infection of feline macrophages was studied using monoclonal antibodies (mabs) to the fipv strain 79-1146. adherent cells recovered from the feline lung and peritoneal cavity phagocytosed fixed red blood cells, and formed fc-mediated rosettes. enhancement of virus infection by mab was investigated by inoculating alveolar macrophages with a mixtures of viral suspension and mab, and examining the cells for intracellular viral antigen by the ... | 1991 | 1659798 |
| characterization of crystals of an fab fragment of a murine monoclonal antibody. | the fab fragment of an antibody, made against an e2-specific feline infectious peritonitis virus neutralizing antibody, has been crystallized in a form suitable for x-ray diffraction analysis from peg 4000 using vapor diffusion methods. the fab fragment crystals diffract to about 2.9 a resolution and are of triclinic space group p1. unit cell dimensions, by which the reciprocal lattice can be indexed, are a = 57.16 a, b = 70.85 a, c = 75.81 a, alpha = 85.11 degrees, beta = 121.28 degrees and gam ... | 1991 | 1660922 |
| characterization of monoclonal antibodies against feline infectious peritonitis virus type ii and antigenic relationship between feline, porcine, and canine coronaviruses. | seven monoclonal antibodies (mabs) with neutralizing activity against feline infectious peritonitis virus (fipv) strain 79-1149 (type ii) were prepared. when the polypeptide specificity recognized by these monoclonal antibodies (mabs) was investigated by western immunoblotting, all of the mabs reacted with peplomer glycoprotein (s) of the virus. by competitive binding assay these mabs were found to recognize at least 3 different epitopes. the reactivity of these mabs with 6 viruses classified as ... | 1991 | 1706593 |
| differentiation of transmissible gastroenteritis virus from porcine respiratory coronavirus and other antigenically related coronaviruses by using cdna probes specific for the 5' region of the s glycoprotein gene. | two cdna clones prepared from the virulent miller strain of transmissible gastroenteritis virus (tgev) were identified, and their nucleotide sequences were determined. the clones were nonoverlapping and located in the 5' region of the s glycoprotein gene. their nucleotide and predicted amino acid sequences were compared with published sequences of the attenuated purdue strain of tgev and feline infectious peritonitis virus (fipv). tgev clone pe21 contained 381 bp of the s glycoprotein gene and h ... | 1991 | 1847152 |
| primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens. | feline infectious peritonitis virus (fipv) causes a mostly fatal, immunologically mediated disease in cats. previously, we demonstrated that immunization with a recombinant vaccinia virus expressing the fipv spike protein (s) induced early death after challenge with fipv (vennema et al., 1990, j. virol. 64, 1407-1409). in this paper we describe similar immunizations with the fipv membrane (m) and nucleocapsid (n) proteins. the genes encoding these proteins were cloned and sequenced. comparison o ... | 1991 | 1847259 |
| immunogenicity of recombinant feline infectious peritonitis virus spike protein in mice and kittens. | the gene encoding the fusogenic spike protein of the coronavirus causing feline infectious peritonitis (fipv) was recombined into the genome of vaccinia virus, strain wr. the recombinant induced spike protein specific, in vitro neutralizing antibodies in mice. when kittens were immunized with the recombinant, low titers of neutralizing antibodies were obtained. after challenge with fipv, these animals succumbed earlier than the vwr-immunized control group ("early death syndrome"). | 1990 | 1966406 |
| characterization of an attenuated temperature sensitive feline infectious peritonitis vaccine virus. | intranasal administration of a ts-fipv vaccine protected cats against two rigorous challenges of immunity. investigations showed that ts-fip viral rna synthesis was normal at 39 degrees c and structural proteins were synthesized, but not expressed at the cell surface. lack of surface expression combined with decreased virus titer indicate that, although structural viral proteins were initially synthesized, they were not packaged into intact virions at the nonpermissive temperature. the ts-fip va ... | 1990 | 1966441 |
| protection against feline infectious peritonitis by intranasal inoculation of a temperature-sensitive fipv vaccine. | cats vaccinated intranasally (i.n.) with a temperature sensitive feline infectious peritonitis virus (ts-fipv) vaccine were protected against an fip-inducing challenge. seventeen of 20 vaccinated cats (85%) survived a rigorous virulent fipv challenge that caused fip in 12 of 12 non-vaccinated cats (100%), 10 (83%) of which died. intranasal vaccination stimulated serum igg and serum and salivary iga antibody responses (measured by elisa), fipv-neutralizing antibody (vn), and a cell-mediated immun ... | 1990 | 2087874 |
| [the use of genetic engineering in veterinary medicine with examples from epidemiology, diagnosis and drug production]. | the results of genetic engineering have reached practical veterinary medicine already. nevertheless there is a great lack of knowledge among those veterinarians who usually do not work with these methods. therefore we want to give an introduction into the advantages and dangers of this technology concerning veterinary medicine. some important analytical methods are explained. related viruses such as wee and eee or canine parvovirus, feline parvovirus and mink enteritis virus, or the related coro ... | 1990 | 2112273 |
| potentiation of platelet responses in vitro by feline infectious peritonitis virus. | the effect of feline infectious peritonitis virus (fipv) on platelet aggregation and 14c-serotonin release induced by threshold levels of four agonists (adenosine diphosphate [adp], collagen, arachidonic acid, and epinephrine) was examined in vitro in ten specific-pathogen-free cats. purified suspensions of fipv added to stirred platelet suspensions (virus to platelet ratio equal to 1:320) 1 minute prior to the addition of agonist potentiated the adp-induced aggregation response by greater than ... | 1990 | 2144930 |
| early death after feline infectious peritonitis virus challenge due to recombinant vaccinia virus immunization. | the gene encoding the fusogenic spike protein of the coronavirus causing feline infectious peritonitis was recombined into the genome of vaccinia virus. the recombinant induced spike-protein-specific, in vitro neutralizing antibodies in mice. when kittens were immunized with the recombinant, low titers of neutralizing antibodies were obtained. after challenge with feline infectious peritonitis virus, these animals succumbed earlier than did the control group immunized with wild-type vaccinia vir ... | 1990 | 2154621 |
| effect of interferon or propionibacterium acnes on the course of experimentally induced feline infectious peritonitis in specific-pathogen-free and random-source cats. | seventy-four cats (52 treated and 22 untreated) were evaluated in efficacy studies of interferon (ifn), propionibacterium acnes, or a combination of these drugs against experimentally induced feline infectious peritonitis (fip). cats were given doses of recombinant human leukocyte (alpha) ifn (rhuifn-alpha), feline fibroblastic (beta) ifn (fifn-beta) or p acnes at regular intervals before and after inoculation of virulent fip virus (fipv). prophylactic and therapeutic administration of high dose ... | 1990 | 2159739 |
| demonstration of feline corona virus (fcv) antigen in organs of cats suspected of feline infectious peritonitis (fip) disease. | cryosections of organs and smears from membrana nicitians from cats suspected of having spontaneous infection with feline infectious peritonitis virus (fipv), were investigated using an indirect immunofluorescence assay (iifa) in order to detect the presence of feline corona virus (fcv). in 113 cats, from each of which six organs were screened, virus antigen was found most often in membrana nicitians and lung. out of these animals an additional six organs from a group of 30 cats were screened. i ... | 1990 | 2168724 |
| enhanced platelet reactivity in cats experimentally infected with feline infectious peritonitis virus. | platelet function was evaluated in six specific-pathogen-free cats prior to and following intraperitoneal inoculation with feline infectious peritonitis virus (fipv). by 4 days post-inoculation, platelet samples from five of six cats responded with irreversible platelet aggregation to threshold concentrations of adenosine diphosphate (adp). this was accompanied by enhanced platelet 14c-serotonin release (greater than 10%) in two cats. compared to one of six baseline samples, five of five post-in ... | 1990 | 2169665 |
| a case report: encephalitis in lions. pathological and virological findings. | an acute outbreak of encephalomyelitis in lions from a safari-park was investigated. three moribund lions were euthanatized and a post mortem examination was performed. a disseminated non-suppurative polioencephalomyelitis with demyelination in the spinal cord was the only pathological finding. from tonsil material of one lion feline herpesvirus type 1 was isolated. canine distemper virus, pseudorabies virus, feline infectious peritonitis virus and rabies were excluded as cause of the disease. t ... | 1990 | 2311536 |
| nucleotide sequence of the gene encoding the spike glycoprotein of human coronavirus hcv 229e. | the gene encoding the spike glycoprotein of the human coronavirus hcv 229e has been cloned and sequenced. this analysis predicts an s polypeptide of 1173 amino acids with an mr of 128,600. the polypeptide has 30 potential n-glycosylation sites. a number of structural features typical of coronavirus s proteins can be recognized, including a signal sequence, a membrane anchor, heptad repeat structures and a carboxy-terminal cysteine cluster. a detailed, computer-aided comparison with the s protein ... | 1990 | 2345367 |
| intracellular transport of recombinant coronavirus spike proteins: implications for virus assembly. | coronavirus spike protein genes were expressed in vitro by using the recombinant vaccinia virus expression system. recombinant spike proteins were expressed at the cell surface and induced cell fusion in a host-cell-dependent fashion. the intracellular transport of recombinant spike proteins was studied. the half time of acquisition of resistance to endo-beta-n-acetylglucosaminidase h was approximately 3 h for the recombinant feline infectious peritonitis virus s protein. the s protein in feline ... | 1990 | 2403441 |
| antigenic structure of transmissible gastroenteritis virus. i. properties of monoclonal antibodies directed against virion proteins. | thirty-two hybridoma cell lines producing monoclonal antibodies (mabs) against the three major structural proteins of transmissible gastroenteritis virus (tgev) have been isolated. radioimmunoprecipitation of intracellular viral polypeptides showed that 17 hybridomas recognized both the peplomer protein [e2, 220 x 10(3) mol. wt. (220k)] and a lower mol. wt. species (e'2, 175k), which was characterized as a precursor of e2. six mabs selectively immunoprecipitated the e'2 protein. four hybridomas ... | 1986 | 2418148 |
| epitope-specific antibody responses to virulent and avirulent feline infectious peritonitis virus isolates. | feline infectious peritonitis virus (fipv) has been isolated several times from infected cats. some of these isolates vary markedly in their ability to cause disease. specific-pathogen-free cats were inoculated with the avirulent fipv-ucd-2 isolate or the extremely virulent fipv-79-1146 isolate or both. after 1 month, cats which had received fipv-79-1146 were either dead or showed clinical signs of fip. all cats which received only fipv-ucd-2 remained healthy up to 6 months after inoculation. an ... | 1987 | 2442191 |
| humoral immune response of cats to virulent and avirulent feline infectious peritonitis virus isolates. | isolates of feline infectious peritonitis virus (fipv) vary in their degree of virulence and have antigenically and functionally different peplomer glycoproteins. we tested the most virulent isolate, fipv-79-1146 and the avirulent isolate, fipv-ucd-2 in vivo in order to better understand the pathogenesis of fip. specific pathogen free cats were inoculated with either fipv-79-1146 or fipv-ucd-2. after 28 days cats which had received fipv-79-1146 were either dead or showing clinical signs of fip. ... | 1987 | 2449048 |
| cats inoculated with feline infectious peritonitis virus exhibit a biphasic acute phase plasma protein response. | | 1988 | 2464231 |
| comparative features of a coronavirus isolated from a cheetah with feline infectious peritonitis. | a coronavirus which was isolated from a cheetah (acinonyx jubatus) that succumbed to feline infectious peritonitis was characterized in vitro. the virus was determined to be highly cell-associated with crandell feline kidney (crfk) cells and was routinely maintained as a persistent infection (crfk 83-4497). the cheetah coronavirus was compared with other members of the feline coronavirus group including the feline enteric coronavirus (fecv) 79-1683 and the feline infectious peritonitis viruses ( ... | 1989 | 2546331 |
| stably expressed fipv peplomer protein induces cell fusion and elicits neutralizing antibodies in mice. | we have established bovine papilloma virus (bpv)-transformed mouse c127 cell lines that synthesize the peplomer protein of the feline infectious peritonitis virus (fipv) strain 79-1146. for this purpose, a new cassette expression vector phsl, which carries the drosophila hsp70 promotor and the polyadenylation signal of the moloney murine leukemia virus long terminal repeat, was constructed. cocultivation of the bpv-transformed cell lines with fipv-permissive feline fcwf-d cells resulted in polyk ... | 1989 | 2548329 |
| inhibitory effects of ribavirin alone or combined with human alpha interferon on feline infectious peritonitis virus replication in vitro. | the antiviral activities of ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide; virazole), either alone or in combination with recombinant human leukocyte (alpha) interferon (rhuifn-alpha), were evaluated against feline infectious peritonitis virus (fipv) in feline kidney-cell cultures. the 50% inhibitory dose (id50) of ribavirin for uninfected, rapidly dividing cells was approximately 17 micrograms ml-1 whereas the id50 for fipv was 2.5 micrograms ml-1. the therapeutic index (ti) of ... | 1989 | 2549687 |
| demonstration of feline infectious peritonitis virus in conjunctival epithelial cells from cats. a simple and reliable method for clinical veterinary virology screening. | a simple technique demonstrating the presence of feline infectious peritonitis virus (fipv) in cats is described. smears from the third eyelid (membrana nictitans) were dried on microscope slides, fixed in aetone, and stained using an indirect immunofluorescence method with rabbit-anti-fipv serum. by this method fipv-antigen could be demonstrated in the cytoplasma of epithelial cells. the results obtained with this method (m3) were compared with serological and immunohistopathological methods fo ... | 1989 | 2551353 |
| evaluation of immunity to feline infectious peritonitis in cats with cutaneous viral-induced delayed hypersensitivity. | delayed-type hypersensitivity (dth)-like reactions to feline infectious peritonitis (fip) virus (fipv) were induced in the skin of nine cats that were asymptomatic after a previous challenge-exposure with fipv. four of the nine previously challenge-exposed cats were negative for virus-neutralizing antibodies against fipv at the time of intradermal (id) testing for dth. two other cats tested for dth when acutely ill with clinical fip did not have cutaneous dth responses to fipv. gross skin reacti ... | 1989 | 2552650 |
| characterization of a temperature sensitive feline infectious peritonitis coronavirus. | the characteristics of a temperature sensitive feline infectious peritonitis virus (ts-fipv) were examined. ts-fipv, unlike its parent strain, df2 wild type fipv (wt-fipv), propagated at 31 degrees c (permissive temperature) but not at 39 degrees c (nonpermissive temperature). this temperature preference of ts-fipv was also demonstrated in cats by the ability of the virus to replicate only at the lower temperature in the upper respiratory tract and not at systemic sites where higher temperatures ... | 1989 | 2558634 |
| evaluation of antithrombin-iii activity as a coindicator of disseminated intravascular coagulation in cats with induced feline infectious peritonitis virus infection. | six adult specific-pathogen-free cats were inoculated intraperitoneally with a cell culture-adapted strain of feline infectious peritonitis virus. plasma samples were evaluated for antithrombin-iii (at-iii) activities at post-inoculation days (pid) 0, 4, and 11 and at termination on pid 16 (1 cat) or 21 (5 cats). other hemostatic values evaluated were activated partial thromboplastin times, prothrombin times, thrombin times, fibrinogen, platelet counts, and fibrin/fibrinogen degradation products ... | 1989 | 2559630 |
| clinical feline toxoplasmosis. serologic diagnosis and therapeutic management of 15 cases. | clinical toxoplasmosis was diagnosed in 15 cats by correlating serologic evidence of infection and clinical signs to either response to therapy or histopathologic demonstration of the organism. ophthalmic manifestations, primarily involving the anterior segment, were common. other common physical examination abnormalities included muscle hyperesthesia, fever, and weight loss. response to therapy was variable, but administration of clindamycin hydrochloride resulted in resolution of all clinical ... | 1989 | 2778747 |
| isolation and characterization of a new and high titer strain of feline infectious peritonitis virus. | | 1987 | 2828734 |
| rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (elisa). | the development of three separate rapid elisas for detecting antibodies in host serum to three different viruses is described. these include: 1. a direct antigen assay using enzyme labelled anti-canine ig for detecting antibodies to canine parvovirus, 2. a competitive elisa using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. a competitive elisa using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26 ... | 1987 | 2829416 |
| the nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus (tgev): comparison with the sequence of the peplomer protein of feline infectious peritonitis virus (fipv). | the amino acid sequence of the peplomer protein of transmissible gastroenteritis virus (tgev) has been derived from the cloned cdna sequence. the gene encodes a protein of 1447 amino acids with a molecular weight of 159 574. comparison with the primary structure of the peplomer protein of feline infectious peritonitis virus (fipv) (de groot et al., 1987b) revealed one domain, from amino acids 1 to 274, in which the nucleotide homology was 39%, whereas in the second domain (from residues 275 to 1 ... | 1987 | 2829461 |
| relatedness of rabbit coronavirus to other coronaviruses. | rabbit coronavirus (rbcv) was apparently first encountered in 1961 when scandinavian investigators observed occasional mortality in rabbits used to propagate the nichols strain of treponema pallidum. mortality rates reached 50 percent by 1968 and 75 percent by 1970. contaminated samples of t. pallidum were brought to the johns hopkins university school of medicine, a world health organization center for the study of treponematoses. there it was established that the causative agent was filterable ... | 1987 | 2829566 |
| virologic and immunologic aspects of feline infectious peritonitis virus infection. | a number of feline coronavirus isolates have been characterized over the last few years. these isolates consist of what we have referred to as feline enteric coronaviruses (fecvs) and feline infectious peritonitis viruses (fipvs). fecvs cause a transient enteritis in kittens but no systemic illness. fipvs, in contrast, cause a systemic and usually fatal disease syndrome characterized either by an exudative serositis or a disseminated granulomatous disease. although the diseases they cause are qu ... | 1987 | 2829567 |
| feline interleukin 1 production induced by feline infectious peritonitis virus. | | 1988 | 2834596 |
| a titration method of feline infectious peritonitis virus using immunoperoxidase antibody. | | 1988 | 2834597 |
| virus shedding and immune responses in cats inoculated with cell culture-adapted feline infectious peritonitis virus. | eight specific pathogen-free cats were inoculated orally or parenterally with a cell culture-adapted strain of feline infectious peritonitis virus (fipv). faeces and oropharyngeal swabs were monitored daily for infectious virus by inoculation of feline embryo lung cells. virus was recovered from both sites for approximately 2 weeks after inoculation, before clinical signs of disease developed. peripheral blood lymphocytes collected from these cats were tested in an in-vitro blastogenic assay usi ... | 1988 | 2836990 |
| delayed-type hypersensitivity skin responses associated with feline infectious peritonitis in two cats. | two cats previously challenge-exposed and seropositive to feline infectious peritonitis virus (fipv) were evaluated for delayed-type hypersensitivity (dth) skin responses to intradermal fipv. before testing, cat 1 (fip-resistant) had survived a severe experimental fipv challenge-exposure and had remained asymptomatic, whereas cat 2 (fip-susceptible) developed acute fulminant fip after a considerably smaller virus challenge-exposure. cat 1 developed a focal thickened plaque at the fipv-injected s ... | 1988 | 2841735 |
| increased plasma levels of leukotriene b4 and prostaglandin e2 in cats experimentally inoculated with feline infectious peritonitis virus. | specific-pathogen-free kittens experimentally infected with feline infectious peritonitis virus (fipv) subsequently demonstrated increased plasma levels of the arachidonic acid metabolites, leukotriene (lt) b4 and prostaglandin (pg) e2. significant increases (p less than 0.025) in ltb4 plasma levels occurred in all (5/5) fipv-inoculated kittens on postchallenge-exposure days (pcd) 7 and 14 vs pcd 0. significant increases (p less than 0.05) in pge2 plasma levels occurred in 80% (4/5) of fipv-infe ... | 1988 | 2848354 |
| biological and pathological consequences of feline infectious peritonitis virus infection in the cheetah. | an epizootic of feline infectious peritonitis in a captive cheetah population during 1982-1983 served to focus attention on the susceptibility of the cheetah (acinoyx jubatus) to infectious disease. subsequent observations based upon seroepidemiological surveys and electron microscopy of fecal material verified that cheetahs were indeed capable of being infected by coronaviruses, which were antigenically related to coronaviruses affecting domestic cats, i.e. feline infectious peritonitis virus/f ... | 1988 | 2849387 |
| attempted immunisation of cats against feline infectious peritonitis using canine coronavirus. | specific pathogen free kittens were vaccinated with an unattenuated field isolate of canine coronavirus (ccv) either by aerosol or subcutaneously, and received boosting vaccinations four weeks later. aerosolisation elicited a homologous virus-neutralising (vn) antibody response that increased steadily over a four-week period and levelled off one to two weeks after revaccination. the initial aerosolised dose produced an asymptomatic infection with excretion of ccv from the oropharynx up to eight ... | 1988 | 2850601 |
| comparison of serologic assays for measurement of antibody response to coronavirus in cats. | serologic virus neutralization tests, indirect immunofluorescence tests, and elisa, using tissue culture-adapted feline infectious peritonitis virus (fipv) or feline enteric coronavirus (fecv) were compared for their ability to distinguish specific virus exposure in cats. sera of specific-pathogen-free cats inoculated with virulent or modified fipv or fecv were used to compare the sensitivity and specificity of the homologous assays to a heterologous assay that measures antibody reactivity with ... | 1988 | 2851952 |
| the sites of early viral replication in feline infectious peritonitis. | the sites of early replication of feline infectious peritonitis virus were studied following oral inoculation of specific-pathogen-free (spf) cats with virus grown in cell cultures. viral antigen was first detected by immunofluorescence in the tonsils and small intestine within 24 h of inoculation, and was later found in caecum, colon, mesenteric lymph nodes and liver. however, histological changes in the gut did not appear until relatively late in the course of infection. virus was recovered fr ... | 1988 | 2852873 |
| role of t cells in feline infectious peritonitis virus infection of suckling mice. | | 1985 | 2993724 |
| experimental inoculation of cats with human coronavirus 229e and subsequent challenge with feline infectious peritonitis virus. | minimal-disease cats exposed to live human coronavirus 229e developed homologous antibody responses that suggested little or no replication of the virus in inoculated animals. oronasal and subcutaneous inoculation of coronavirus 229e did not elicit an antibody response by heterologous (transmissible gastroenteritis virus, canine coronavirus) neutralization or by heterologous (transmissible gastroenteritis virus) kinetics-based enzyme-linked immunosorbent assay. no clinical signs attributable to ... | 1985 | 2994865 |
| competitive enzyme immunoassays for the rapid detection of antibodies to feline infectious peritonitis virus polypeptides. | monoclonal antibodies specific for the envelope (e1), peplomer (e2), and nucleocapsid (n) polypeptides of feline infectious peritonitis virus (fipv) were used in rapid, competitive enzyme-linked immunosorbent assays (elisa) to study the humoral immune response of cats to fipv infection. results from the competitive elisas were correlated with those from immunofluorescent antibody assays (ifas) on 203 samples obtained from 64 individual cats. the ifa results correlated best with those obtained wi ... | 1985 | 2995437 |
| virion polypeptide specificity of immune complexes and antibodies in cats inoculated with feline infectious peritonitis virus. | immune complexes purified from sera and ascitic fluids of cats after inoculation with feline infectious peritonitis (fip) virus contained proteins and proteolytic fragments of the peplomer, nucleocapsid, and envelope polypeptides; in addition, host proteins were demonstrated in the immune complexes. free (uncomplexed) antibodies against the 3 classes of virion polypeptides were detected and quantitated; the weakest and latest response was directed against the peplomer protein. immunofluorescence ... | 1986 | 3008607 |
| intracellular rnas of the feline infectious peritonitis coronavirus strain 79-1146. | in felis catus whole foetus d cells infected with feline infectious peritonitis virus (fipv), strain 79-1146, six virus-specific, poly(a)-containing rna species of about 20, 9.6, 5.2, 3.8, 2.8 and 1.6 kb were found. by translation in vitro the 3.8 and 2.8 kb rnas were shown to encode the 25k envelope protein and the 45k nucleocapsid protein, respectively. the partial map of the fipv genome was compared with genomic maps of porcine, murine and avian coronaviruses. differences in these maps sugges ... | 1987 | 3033137 |
| comparative properties of feline coronaviruses in vitro. | two feline coronaviruses were characterized to determine their biological properties in vitro and their antigenic relatedness to a previously recognized feline infectious peritonitis virus and canine coronavirus. the viruses, designated wsu 79-1146 and wsu 79-1683, were shown to have comparable growth curves with the prototype feline infectious peritonitis virus. treatment of the feline infectious peritonitis virus strains with 0.25% trypsin indicated that they were relatively resistant to prote ... | 1987 | 3038290 |
| effects of elevated temperatures on the opacity and toxicity of pertussis vaccines manufactured with different inactivating agents. | plain pertussis vaccine manufactured with different inactivating agents was evaluated for its stability at 4-8, 25 and 35 degrees c with regard to the opacity and the toxicity [mouse weight gain test (mwgt) and histamine sensitizing (hs) activity]. two pools each of heat inactivated pertussis vaccine (hipv), formaldehyde inactivated pertussis vaccine (fipv), glutaraldehyde inactivated pertussis vaccine (gipv), thimerosal inactivated pertussis vaccine (tipv) and acetone (i) treated pertussis vacc ... | 1986 | 3094265 |
| inhibition of feline infectious peritonitis virus replication by recombinant human leukocyte (alpha) interferon and feline fibroblastic (beta) interferon. | replication of feline infectious peritonitis virus (fipv) in feline cell cultures was inhibited after incubation of cells with either human recombinant leukocyte (alpha) interferon (ifn) or feline fibroblastic (beta) ifn for 18 to 24 hours before viral challenge exposure. compared with virus control cultures, fipv yields were reduced by ranges of 0.1 to 2.7 log10 or 2 to 5.2 log10 tcid50 in cultures treated with human alpha- or feline beta-ifn, respectively; yield reductions were ifn dose depend ... | 1988 | 3178028 |
| isolation and identification of feline peritoneal macrophages for in vitro studies of coronavirus-macrophage interactions. | feline peritoneal cells were collected by lavage with isotonic saline without the use of irritants or need for euthanasia of the cats. macrophages were purified by centrifugation on percoll followed by selective adherence. although few macrophages could be obtained from an initial lavage, a second lavage performed on the same cat 9-11 days later yielded six times as many macrophages as the first lavage, providing sufficient numbers of cells for characterization and infection experiments. macroph ... | 1988 | 3183510 |
| porcine epidemic diarrhea virus (cv 777) and feline infectious peritonitis virus (fipv) are antigenically related. | using gut sections from pigs infected with porcine epidemic diarrhea virus (strain cv 777) and ascitic fluid from cats which had succumbed to feline infectious peritonitis (fip), a weak cross reaction was found by immunofluorescence. its specificity was confirmed when detergent-treated purified cv 777 showed a prominent reaction with fipv antibodies in elisa; no reaction was obtained with intact virions, which indicated common determinants on an internal component of the particle. antigenic cros ... | 1988 | 3196169 |
| sequence analysis of the 3'-end of the feline coronavirus fipv 79-1146 genome: comparison with the genome of porcine coronavirus tgev reveals large insertions. | the genetic information, carried on mrna 6 of feline infectious peritonitis virus (fipv) strain 79-1146, was determined by sequence analysis of cdna clones derived from the 3' end of the fipv genome. two orfs were found, encoding polypeptides of 11k (orf-1) and 22k (orf-2). the fipv sequence was compared to the 3' end sequence of transmissible gastroenteritis virus (tgev). orf-1 has a homologous counterpart (orf-x3) in the tgev genome; both orfs are located at the same position relative to the n ... | 1988 | 3201747 |
| identification of viral antigens that induce antibody responses on exposure to coronaviruses. | various techniques were used to look for protective, non-cross-reactive antibodies in the sera of cats exposed to virulent feline infectious peritonitis virus (fipv). antibodies reactive with feline enteric coronavirus (fecv) from fipv-exposed cats were adsorbed by several passages over an fecv-sepharose column. in an elisa against fecv and fipv, the activity against both viruses was removed at the same rate; thus, no fipv-specific antibodies could be identified. by gel electrophoresis-derived e ... | 1988 | 3223654 |
| cdna cloning and sequence analysis of the gene encoding the peplomer protein of feline infectious peritonitis virus. | the peplomer gene of feline infectious peritonitis virus (fipv) strain 79-1146 was isolated from a genomic cdna library by differential hybridization with rna 2 and 3 as probes. from the nucleotide sequence a primary translation product of 1452 residues (mr 160,472) was predicted, containing an n-terminal signal sequence, a c-terminal transmembrane segment and 35 potential n-linked glycosylation sites. by s1 nuclease analysis the 5' end of the presumptive rna 2 body was located at about 30 nucle ... | 1987 | 3312491 |
| possible association of thymus dysfunction with fading syndromes in puppies and kittens. | "wasting" or "fading" syndromes are common causes of puppy and kitten mortality. numerous infectious and toxic, metabolic, or nutritional factors could potentially be responsible for wasting and death in young animals. evidence has been presented that infectious canine hepatitis virus infection, beta-hemolytic streptococcus infection, and feline infectious peritonitis virus infection are responsible for a significant number of deaths due to wasting syndrome. however, many cases of wasting syndro ... | 1987 | 3496704 |
| feline infectious peritonitis virus antibody test using enzyme-linked immunosorbent assay. | | 1987 | 3573475 |
| antigenic comparison of feline coronavirus isolates: evidence for markedly different peplomer glycoproteins. | the antigenic relationships among seven feline coronavirus isolates were investigated by using a panel of 26 monoclonal antibodies (mabs). the mabs were categorized into five immunoreactive groups which were used to delineate two antigenic types of feline coronaviruses. one antigenic type included the more virulent feline infectious peritonitis virus (fipv) isolates (fipv-ucd-1, fipv-ucd-4, fipv-tn406, fipv-df2, and fipv-79-1146), whereas the second antigenic type was composed of the avirulent i ... | 1987 | 3599183 |
| functional differences in the peplomer glycoproteins of feline coronavirus isolates. | the feline coronaviruses can be divided into two distinct antigenic groups on the basis of antigenic differences found on the peplomer (e2) glycoprotein of the virus. because the e2 glycoprotein is responsible for many of the biological functions of coronaviruses, experiments were done to determine whether there were any e2 functional differences between these two antigenic groups. the avirulent feline infectious peritonitis virus (fipv) isolate fipv-ucd-2, which has one antigenic type of e2, wa ... | 1987 | 3599185 |
| antigenic and biological diversity of feline coronaviruses: feline infectious peritonitis and feline enteritis virus. | antigenically related feline coronaviruses cause two distinct disease manifestations in infected cats. the diseases are feline infectious peritonitis (fip), in which the virus is widely disseminated, and feline enteric coronavirus (fecv), a mild disease in which the virus is usually limited to the villi. these two viruses were found to differ in their growth in cell culture. fipv grows to higher titer, forms larger plaques and switches off host cell protein synthesis more effectively than fecv. ... | 1987 | 3619653 |
| pathogenicity studies of feline coronavirus isolates 79-1146 and 79-1683. | two feline coronavirus isolates were characterized by their disease-causing potential in cats. the 79-1683 feline coronavirus isolate caused an inapparent-to-mild enteritis when given oronasally to specific-pathogen-free kittens and was not a cause of feline infectious peritonitis (fip). target tissues for the virus were the mature apical epithelium of the small intestine, mesenteric lymph nodes, tonsils, thymus, and (to a lesser extent) the lungs. inoculated kittens shed high numbers of virus i ... | 1984 | 6084432 |
| role of neutrophils in production of early enteritis after oral infection with feline infectious peritonitis virus in passively sensitized kittens. | | 1984 | 6090746 |
| feline infectious peritonitis. an immune-mediated coronaviral vasculitis. | mainly through studies inducing experimental infection of susceptible cats, significant advances have recently been made in our understanding of the pathogenesis of fip. much of this knowledge should not presently be directly extrapolated to field cases of fip, because the route of infection and challenge dose and strain of virus may be significantly different. advances in the prevention and treatment of fip will depend greatly on clarification of the exact nature of the several coronaviruses af ... | 1984 | 6093323 |
| experimental inoculation of cats with canine coronavirus and subsequent challenge with feline infectious peritonitis virus. | oronasal inoculation of minimal-disease cats with a virulent field isolate of canine coronavirus elicited a neutralizing antibody response that increased steadily over a two-month period, indicating in vivo amplification of the inoculated dose. a heterologous neutralizing antibody response to transmissible gastroenteritis virus could be detected three weeks after titers to canine coronavirus were first observed and was found to parallel the homologous response at a level approximately one log10 ... | 1984 | 6097763 |
| an immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, cv 777, and several coronaviruses. | a possible antigenic relationship between the porcine enteropathogenic coronavirus-like agent (cvla) and 6 known coronaviruses was examined by immunoelectron microscopy (iem) and by immunofluorescence (if). cvla did not show cross reactivity with infectious bronchitis virus, transmissible gastroenteritis virus (tgev), canine coronavirus (ccv) hemagglutinating encephalomyelitis virus (hev), neonatal calf diarrhea coronavirus (ncdcv) or feline infectious peritonitis virus (fipv). antigenic relatio ... | 1981 | 6166280 |
| lesions in the small intestine of newborn pigs inoculated with porcine, feline, and canine coronaviruses. | the infectivity and pathogenicity to newborn pigs of antigenically related coronaviruses from pigs (transmissible gastroenteritis virus; tgev), cats (feline infectious peritonitis virus; fipv), and dogs (canine gastroenteritis virus; cgev) were studied by light, scanning electron, and immunofluorescence microscopy. hysterectomy-derived, 12-hour-old pigs were orally given tissue culture or frozen preparations of 6 coronavirus strains (3 porcine, 2 feline, and 1 canine). the pigs were killed at re ... | 1981 | 6168221 |
| antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses. | transmissible gastroenteritis virus of swine (tgev), feline infectious peritonitis virus (fipv), and canine coronavirus were studied with respect to their serological cross-reactivity in homologous and heterologous virus neutralization, immune precipitation of radiolabeled tgev, electroblotting, and enzyme-linked immunosorbent assay using individual virion polypeptides prepared by polyacrylamide gel electrophoresis. tgev was neutralized by feline anti-fipv serum, and the reaction was potentiated ... | 1982 | 6182101 |
| disseminated intravascular coagulation in experimentally induced feline infectious peritonitis. | disseminated intravascular coagulation was induced in kittens by intraperitoneal inoculation of feline infectious peritonitis virus (fipv). kittens seronegative to fipv survived significantly (p less than 0.05) longer than those seropositive to fipv. pyrexia, anemia, icterus, hyperbilirubinemia, and elevated concentrations of liver-specific enzymes were detected in the inoculated cats. lesions induced included disseminated fibrinonecrotic and pyogranulomatous inflammation, hepatic necrosis, and ... | 1980 | 6250426 |
| immunofluorescent and electron microscopic studies of feline small intestinal organ cultures infected with feline infectious peritonitis virus. | the pathogenesis, morphology, and morphogenesis of feline infectious peritonitis virus were studied in feline small intestinal organ cultures. characteristic coronaviruses were demonstrated by electron microscopy in the extracellular organ culture fluid taken at 1 through 12, 15, 22, 31, and 43 days after the cultures were inoculated. viral particles had a mean diameter of 94 nm. numerous petal-shaped projections approximately 15 nm long were seen on the envelopes. specific immunofluorescence wa ... | 1980 | 6250427 |
| in vitro culture of feline infectious peritonitis virus. | | 1981 | 6267766 |
| infection studies in kittens, using feline infectious peritonitis virus propagated in cell culture. | the propagation of feline infectious peritonitis virus (nw1-fipv strain) in cell culture is described. tissue culture-propagated virus was used to inoculate specific-pathogen-free kittens intraperitoneally, intratracheally, or orally. intraperitoneal inoculation caused seroconversion and effusive peritonitis in 100% of the kittens. intratracheal inoculation produced disease in 60% of the kittens, and oral inoculation in only 20%. seroconversions without production of disease occurred in 10% of t ... | 1981 | 6267959 |
| an enteric coronavirus infection of cats and its relationship to feline infectious peritonitis. | an enteric coronavirus that is antigenically closely related to feline infectious peritonitis virus (fipv) is ubiquitous in the cat population. this virus has been designated feline enteric coronavirus to differentiate it from fipv. the virus is shed in the feces by many seropositive cats; in catteries it is a cause of inapparent to mildly severe enteritis in kittens 6 to 12 weeks of age. the virus may produce a more severe enteritis in young specific-pathogen-free kittens. feline enteric corona ... | 1981 | 6267960 |
| pathogenesis of feline infetious peritonitis: pathologic changes and immunofluorescence. | feline infectious peritonitis (fip) was experimentally induced in fip virus (fipv) antibody-positive and antibody-negative kittens after challenge exposure to live-virus aerosol. seropositive kittens developed antiviral immunofluorescence and lesions more rapidly after challenge exposure than did seronegative kittens. in seropositive kittens, fipv antigen was present in macrophages and large mononuclear cells in tracheobronchial lymph nodes, lungs, and trachea on postchallenge-exposure day (pcd) ... | 1981 | 6280518 |
| antibody, immune complexes, and complement activity fluctuations in kittens with experimentally induced feline infectious peritonitis. | humoral changes were studied in 6 specific-pathogen-free kittens during experimental infection with feline infectious peritonitis virus. although the incubation period and the duration of the disease differed widely, a similar pattern of rectal temperatures, serum complement values, circulating immune complexes, and antibody titers was found for all kittens during the last 15 to 20 days of life. antibody formation started 8 to 13 days before death and was accompanied by the appearance of circula ... | 1982 | 6280534 |
| enteritis due to feline infectious peritonitis virus. | | 1982 | 6285056 |