| structural proteins of bovine coronavirus strain l 9: effects of the host cell and trypsin treatment. | the polypeptide profile of the cell-adapted strain of bovine coronavirus (mebus bcv-l 9) is remarkably affected by the host cell and trypsin. we compared the structural proteins of virus purified from different cell lines and found cell-dependent differences in the virus structure. bcv was purified from four clones of human rectal tumour cells (hrt-18): 3f3, d2, 3e3, and 4b3. the structural profiles of bcv propagated in clones 3e3 and 3f3 were identical, consisting of proteins with molecular wei ... | 1988 | 2463821 |
| bovine coronavirus hemagglutinin protein. | treatment of purified bovine coronavirus (mebus strain) with pronase destroyed the integrity of virion surface glycoproteins gp140, gp120, gp100, reduced the amount of gp26 and destroyed the hemagglutinating activity of the virus. bromelain, on the other hand, destroyed the integrity of gp120, gp100 and gp26 but failed to remove gp140 and failed to destroy viral hemagglutinating activity. these experiments suggest that gp140 is the virion hemagglutinin. immunoblotting studies using monospecific ... | 1985 | 3984492 |
| antigenic relationships among proteins of bovine coronavirus, human respiratory coronavirus oc43, and mouse hepatitis coronavirus a59. | antisera prepared against each of three single and one pair of major structural proteins of the bovine coronavirus (mebus strain) were used in immunoblotting studies to measure cross-reactivity with the structural proteins of the human coronavirus oc43 and the mouse hepatitis coronavirus a59. we conclude that the bovine coronavirus is comprised of four major structural proteins, gp190 (normally present as 120- and 100-kilodalton subunits), gp140, pp52, and gp26. the human coronavirus oc43 has an ... | 1984 | 6205167 |