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the first epidemic of japanese encephalitis studied in india--virological studies. 19752552
equine viral encephalitis.the most important neurotropic viral infections of the horse are the arthropod-borne encephalitides. these include venezuelan encephalitis (ve), eastern encephalitis (ee) and western encephalitis (we), which are found in the americas, and japanese b encephalitis which occurs in the far east. all the viruses cause encephalitis in man. between 1969 and 1972 an epidemic of ve occurred in central america. in 1971 the disease was reported in texas, where it was brought under control by the vaccinatio ...19764301
isolation of japanese encephalitis virus from mosquitoes near bogor, west java, indonesia. 19754621
japanese encephalitis virus from pigs and mosquitoes in jakarta, indonesia.japanese encephalitis virus was isolated from 11 pools of culex tritaeniorhynchus and a single pool of c. gelidus mosquitoes at a pig-raising area near jakarta, west java, indonesia, during 1972-74. ten sentinel pigs placed in the area all developed haemagglutination-inhibition antibodies against japanese encephalitis and the virus was isolated from the blood of 3.19757033
japanese encephalitis virus multiplication in the regional rabbit ear vein in the early viremic period. 197715444
plaque formation by alphavirus (getah) in culex mosquito cells.plaques were formed by getah virus (an alphavirus) which had been grown in bhk cells and passaged once in culex cells before inoculation of culex cell cultures. plaques were not produced either by getah or japanese encephalitis (je) viruses which had been passaged only in bhk cells. plaque counts in culex cell monolayers were almost the same as in bhk cells.197717288
non-infectious antigen for laboratory diagnosis of japanese encephalitis.sucrose-acetone suckling mouse brain antigens of na-kayama nih, jagar-01 and peking-1 strains of japanese encephalitis (je) virus treated with beta-propiolactone (bpl) retained their ability to specifically react in haemagglutination inhibition (hi) tests with antibody to je virus, as demonstrated with convalescent sera. bpl treatment abolished the infectivity but did not affect the haemagglutinating activity of the virus of the ph optimum of the haemagglutination (ha) test.197720767
mononuclear cells in japanese encephalitis virus infection: changes in cells counts and specific fluorescence.progressive reduction in blood cell counts was observed in mice inoculated intracerebrally (ic) with japanese encephalitis (je) virus. no changes were observed in the blood cell counts of mice inoculated intraperitoneally (ip). reduction in cell counts after a transient rise was noticed in lymph nodes of mice inoculated by either route but the cell counts returned to normal in lymph nodes of ip inoculated mice by the 8th day post inoculation (p.i.). je virus antigen was demonstrated by immunoflu ...197722237
agglutination and detection of japanese encephalitis virus-infected lymph node cells with concanavalin a. 197722241
susceptibility of four species of mosquitoes to japanese encephalitis virus as examined by larval infection (author's transl). 197723607
infestation of mosquitoes from the maritime territory by the entomopathogenic fungus, coelomomyces iliensis, under laboratory conditions.the infection of larvae of culex pipiens molestus, c. vagans and c. tritaeniorhynchus (the vector of the virus of japanese encephalitis) with the entomopathogenic fungus coelomomyces iliensis from kazakhstan was carried out in the primorje territory in 1973--1974. at the infection of larvae of c. tritaeniorhynchus with the suspension of infected larvae the mortality was recorded only from 60% of individuals, while at the infection with a content of sporangia--from 36.6% of larvae. somewhat less ...197827745
the isolation of japanese encephalitis virus from taiwan mosquitoes by mosquito cell cultures and mouse inoculation. 197829127
cross reactions among flaviviruses in macrophage migration inhibition assay.adult swiss albino mice were immunized intravenously with flaviviviruses. cellular immune response was studied by macrophage migration inhibition assay. migration of cells from sensitized mice was significantly inhibited in the presence of homologous virus or antigen. migration inhibition was also significant with serologically related viruses, thus establishing cross reaction in cellular immune response.197829477
studies on the mosquitoes of bankura district, west bengal: adult populations. 197831333
isolation of japanese encephalitis virus from mosquitoes collected in bankura district (west bengal) during october 1974 to december 1975. 197934565
virion and soluble antigens of japanese encephalitis virus.japanese encephalitis virions contain a 58 x 10-3-molecular-weight envelope glycoprotein antigen that can be solubilized with sodium lauryl sulfate and separated from other virion structural polypeptides and viral ribonucleic acid by gel filtration chromatography. the 58 x 10-3-molecular-weight envelope protein is the major antigen responsible for cross-reactivity of the virion in complement fixation tests with other closely related arboviruses. a naturally occurring soluble complement-fixing an ...197547312
japanese encephalitis virus plaque formation in culex tritaeniorhynchus cell monolayers. 197549929
[determination of arbovirus group affiliation in the indirect hemagglutination reaction].a dry polyvalent erythrocyte immunoglobulin diagnostic preparation for arboviruses of group b may be prepared to be used in the indirect hemagglutination test. the preparation detects antigens of arboviruses of this group in the tissue culture fluid and in suckling mouse brain tissue treated with sucrose-acetone. the test with group b arbovirus antigens is inhibited specifically by the homologous polyvalent immune gamma globulin. inhibition of hemagglutination by species-specific immune globulin ...197554999
[use of hemagglutination inhibition test for isolation of antibodies against the japanese encephalitis virus]. 197557268
interspecies interactions of arboviruses. i. competition phenomenon in mixed populations of japanese encephalitis and west nile viruses.two phenomena, competition and independent reproduction, were observed on co-cultivation of japanese encephalitis (je) and west nile (wn) viruses in permissive systems. competition occurred in populations propagated in mouse brains and led to elimination of je virus. only clones of wn virus were isolated from these populations at the 1st-3rd passage level. no competition was observed in mixed infection of primary syrian hamster kidney (shk) cell culturess the population had the combined immunolo ...197661719
antigenic characterization among strains of japanese encephalitis virus isolated in hyogo prefecture by the antibody-absorption test. 197664642
[avidity criteria in assessing the functional activity of antigens, antibodies and non-specific serum inhibitors on a model of the kinetic reaction of hemagglutination suppression with arboviruses].the functional activity of some arboviruses of groups a and b, of the antibodies and serum inhibitors was studied on a model of the kinetic hemagglutination inhibition test (hai) by different avidity criteria (velocity, completeness and stability of formation of a neutral complex). the avidity indices of the antigens, antibodies and the inhibitors proved to depend on the group, species and strain peculiarities of the arboviruses, the method of preparation of the antigen, the biological species o ...197665077
interspecies interactions of arboviruses. ii. participation of the genomes of two flaviviruses, west nile and japanese encephalitis, in formation of a virus clone with dual antigenic determinants.a peculiar clone, 41/wn+je+, possessing antigenic determinants of a flaviviruses, west nile (wn) and japanese encphalitis (je), is described. the antigenic duality of this clone exceeded the cross reactions between wn and je viruses; either antiserum neutralized 104--105 pfu/ml of the virus. the property of antigenic duality was inherited in over 340 virus generations and retained upon propagation in selective cell systems and after recloning. the progeny of infectious rna consisted of 3 types o ...197766864
biological and immunological studies on two substrains, c-1 and c-3, derived from the nakayama-nih strain of japanese encephalitis virus.a comparative analysis was performed on two substrains, c-1 and c-3, derived from the nakayama-nih strain of japanese encephalitis virus. the infectivity of the two substrains was inactivated at similar rates by treatment with tween 80 and tween 20 but not by treatment with tween 60. however, hemagglutinating activity as measured at ph 6.8 of the c-3 substrain treated with tween 80 was more labile than that of the c-1 substrain treated with tween 80. also, the immunogenicity (neutralizing and he ...197767102
viruses isolated from bats in japan.virus isolation has been attempted from the blood, spleen, liver and kidneys of bats. nine viruses (1.1 per cent) have been isolated so far from 838 bats collected in different places in japan. two of the isolates (tok-1350 and tok-1362) were isolated from vespertilio supernas collected in fukushima prefecture. they killed suckling mice within 3-5 days after intracerebral inoculation. the remaining seven isolates were all filtrable through millipore filters (0.22 micron) and were pathogenic for ...197768764
antigenic characterization of flavivirus structural proteins separated by isoelectric focusing.isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. the envelope protein and nucleocapsid protein were isolated at isoelectric points of pi 7.8 and 10.3, respectively. the antigenic determinants of st. louis encephalitis, japanese encephalitis, and dengue virus envelope and nucleocapsid proteins were examined by solid-phase competition radioimmunoassay. by the appropriate s ...197769040
clonal analysis of mammalian cell cultures persistently infected with japanese encephalitis virus.more than 200 cells were cloned from populations of mammalian cells persistently infected with japanese encephalitis virus. only four cloned cultures contained cells that had viral antigen measurable by immunofluorescence and that released infectious virus, yet all clones harbored virus-specific rna. superinfection of cloned cells with wild-type japanese encephalitis virus did not produce cytopathic effects, but resulted in production of viral antigen and infectious virus in formerly nonproducin ...1979117119
an attenuated japanese encephalitis vaccine. 1975164051
[effect of japanese encephalitis virus infection on accumulation of methyl mercury in the mouse]. 1975166221
immunological sequelae of trichinella spiralis infection in mice: effect on the antibody responses to sheep erythrocytes and japanse b encephalitis virus.immunosuppression of the antibody response to japanese b encephalitis (jbe) virus and sheep erythrocytes (srbc) was observed in mice infected with trichinella spiralis. this suppression was paralleled by the presence of fewer antibody-forming cells to srbc in the spleens of parasitized mice. both primary and secondary complement-fixing antibody responses to jbe virus were suppressed, but the development of immunological memory was not affected. parasitized mice demonstrated a normal blastogenic ...1975166916
dual infections of mice: visceral larva migrans and sublethal infection with japanese encephalitis virus.experiments in 3 weeks old albino mice with toxocara canis and sublethal infection with je virus established a marked synergestic effect in dually infected mice. the results are discussed to indicate the possible role of visceral larva migrans in creating exploxive outbreaks of "acute encephalopathy syndrome" in individuals having simultaneous infection with a virus (es) which, alone, might produce only mild illness. the nature of the possible mechanisms involved yet remains to be understood.1975167480
origin of brain macrophages and the nature of the so-called microglia.two aspects of the so-called microglia were studied by silver impregnation and 3h-tdr arg in light and electron microscopy. (1) so-called microglioblasts are glioblasts differentiated from matrix cells. they are progenitors of the so-called resting microglia as well as of astrocytes and oligodendroglia. (2) brain macrophages in stab wounds, experimental japanese encephalitis and retrograde degeneration of the facial nucleus are all found to be of hematogenous origin. infiltrating hematogenous ce ...1975168722
experimental transmission of japanese encephalitis virus by culex tritaeniorhynchus and c. fuscocephalus.experimental transmission of japanese encephalitis virus from swine to baby chicks was accomplished using culex tritaeniorhynchus and culex fuscocephalus, two of three mosquito species in taiwan from which the virus has been recovered in nature. whereas neither species had become infective by the twelfth day, one of ten c. fuscocephalus and 11 of 51 c. tritaeniorhynchus were infective by the seventeenth day of extrinsic incubation as verified by viraemia in chicks fed upon by potentially infecti ...1975168819
immunization of pigs with the attenuated s- strain of japanese encephalitis virus.the attenuated s- strain of japanese encephalitis virus was produced from a wild strain of this virus by serial cultivation in primary bovine kidney cell cultures at 30 degrees c. pigs were inoculated with it and examined for ability to produce antibody and protect themselves from infection with a wild strain used for challenge. in pigs inoculated with a single dose of 10(6.5) approximately 10(7.5) tcid50 of the s- strain, the neutralizing antibody titer or hemagglutination-inhibiting antibody ( ...1975170542
arbovirus infections in reptiles: studies on the presence of japanese encephalitis virus antibody in the plasma of the turtle, trionyx sinensis.this study was undertaken to examine further the natural infection of poikilothermic animals e.g. turtles, to japanese encephalitis (je) virus. plasma samples from 75 soft-shelled fresh water turtles (trionyx sinensis wiegman) from china were examined in virus neutralization (vn) and hemagglutination inhibition (hai) tests for the presence of specific antibody. the total incidence of antibody detected by either test to a titer of 10 or greater was 89% while 77% and 60% were positive by vn and ha ...1975170690
hematogenous cells in experimental japanese encephalitis.four adult mice were injected with 3h-thymidine repeatedly so that in their brains only circulating blood cells were labelled with 3h-thymidine. they then received an intracerebral injection of japanese encephalitis virus, were sacrified on the 3rd, 4th and 5th day after inoculation: the brains were examined by light and electron microscopic autoradiography. inflammatory cells appearing in the brain parenchyma and perivascularly in the acute stage of the experimental japanese encephalitis are de ...1975170778
the virulence to man of japanese encephalitis virus in thailand. 1975173200
recent epidemiological features of japanese encephalitis in the republic of korea. 1975173664
the effect of octadecenoic acid on the growth of japanese encephalitis virus in novikoff hepatoma cells.the growth characteristics of japanese encephalitis virus cultivated in novikoff hepatoma cells grown in shaker culture can be differentially altered by the presence of 6-cis or cis-9-octadecenoic acid in swim's 67-g medium. the addition of 125 mug of the 6-isomer per ml medium reduced the number of infectious particles produced, whereas the same amount of the 9-isomer enhanced virus production. the virus was found to be more stable in cell-free spent medium than in fresh medium. the presence of ...1975174140
[study of organ cultures of human embryonic intestines infected with the human cytomegalovirus and the japanese encephalitis virus].reproduction of human cytomegalovirus (cmv) and japanese encephalitis virus (je) was studied in organ cultures of human embryo intestine under conditions of mono- and combined infection. the titer of je virus in monoinfection reached its maximum level (10(7) tcd50/ml) whereas the titer of cmv did not exceed 10(2) tcd50/ml. in combined infection the titer of cmv increased to 10(5.5) tcd50/ml and the titer of je virus remained at the same level (10(7) tcd50/ml). the results obtained in light and s ...1975174325
natural and experimental infections of japanese tree sparrows with japanese encephalitis virus.20 to 37 per cent of wild japanese tree sparrows had neutralizing antibodies to japanese encephalitis virus (jev). sparrows free of antibodies were inoculated with 10,000 plaque-forming units of jev. none of the birds became clinically ill. virus could be detected in blood plasma during the first 3 days after inoculation but not thereafter. neutralizing antibodies started to appear at 4 days after inoculation and the response was of variable duration, in some cases extending up to 200 days after ...1975174531
the effect of dengue virus infection on the clinical sequelae of japanese encephalitis: a one year follow-up study in thailand.in order to determine if prior dengue virus infection reduces the severity of japanese encephalitis (je), we examined 127 patients hospitalized during the 1970 je epidemic in the chiangmai and lampang valleys of northern thailand. patients were studied during the first 30 days after onset of je; 120 of these patients were examined one year later for residual neurologic sequelae. about 21% of patients had serological evidence of a prior dengue virus infection. morbidity and mortality in patients ...1975176736
investigations on the outbreak of japanese encephalitis in burdwan district, west bengal. part ii. serological survey of human population. 1975177357
virological and serological investigations of an epidemic of encephalitis which occurred at bankura district, west bengal. 1976178592
studies on the inhibitory activities of human serum lipoproteins for japanese encephalitis virus.human serum lipoproteins were purified by ultracentrifuging and their concentrations adjusted as required to be within the normal male/female serum range for all assays. the activities in inhibition of hemagglutination (hai) for japanese encephalitis virus were--low density lipoprotein (ldl) greater than very low density lipoprotein (vldl) greater than high density lipoprotein (hdl). heating (56 degrees c/30 minutes) caused the ldl titer to fall and freeze-thawing (20 degrees c/room temperature) ...1975179150
japanese encephalitis surveillance in china (province of taiwan) during 1968-1971. i. geographical and seasonal features of case outbreaks.during 1968-1971 japanese encephalitis (je) surveillance was conducted with who-assisted programs in taiwan area. emphasis was placed on: (1) active case-finding by hospital visits; (2) blood collection from every patient at the suitable time; and (3) the enforcement of standard diagnostic criteria on the results of hemagglutination-inhibition tests. each year, approximately 90% of reported patients were etiologically examined. je virus etiology was established in 277 (1968), 279 (1969), 269 (19 ...1975180319
japanese encephalitis virus in northern taiwan, 1969-1973.sentinel pigs were bled and mosiquitoes were collected for isolation of japanese encephalitis virus (jev) between 1969 and 1973 in a rural area of northern taiwan which reported a high annual incidence of human cases. the study site included a farmyard, schools, a bat cave, rice paddies and a heronry. although culex annulus was collected in every month of the year, isolations were made only in midsummer and only from culex annulus and (once) from culex tritaeniorhynchus. these isolates were usua ...1976180845
host preference of mosquito vectors of japanese encephalitis.the host preference of 4 culex mosquito species collected in miaoli and pingtung counties, taiwan was studied by capillary precipitin method. antisera to alum-precipitated sera of man, bovine, swine, rabbit, horse, dog, cat, mouse, chicken, duck, and pigeon were produced in rabbits and reacted with 758 mosquito blood meals among which reactions to one or more antisera. culex annulus and culex tritaeniorhynchus summorosus showed a great avidity for pig, and culex fuscocephala for bovine. culex pi ...1975181218
efficacy of japanese encephalitis vaccine in horses.the efficacy of japanese encephalitis vaccine in horses has been described from the effect of mass vaccination on the local prevalence of the disease in horses in each district of hokkaido, japan.1976182487
persistence of virus-specific igm and clinical recovery after japanese encephalitis.we have searched for evidence of a chronic japanese encephalitis virus (jev) infection in six thai patients convalescing from acute japanese encephalitis (je) in whom jev-specific igm antibody was last detected 116 to 350 days after their acute illness. these six patients were compared with 94 other je patients matched for age, sex and serological response and in whom jev-specific igm was either short-lived (less than 90 days) or not tested. all patients were evaluated for the presence or absenc ...1976183556
application of freeze-dried, one-day-old chick erythrocytes to viral hemagglutination and hemagglutination-inhibition tests.erythrocytes collected from 1-day-old chicks were stabilized by fixation with formaldehyde and by freeze-drying after treatment with carbon monoxide. suspensions of freeze-dried erythrocytes in distilled water or physiological saline had a homogeneous bright reddish-purple color. freeze-dried erythrocytes were compared with fresh erythrocytes for hemagglutination and hemagglutination-inhibition tests for various viruses including rubella, japanese encephalitis, influenza, mumps, newcastle diseas ...1976184109
experimental studies on vertical infection of mice with japanese encephalitis virus. i. effect of mouse strain on placental and fetal infection.an experiment was carried out with mice to study the effect of the mouse strain on the establishment of experimental vertical infection with japanese encephalitis virus. mice of various inbred strains were inoculated with a field strain of virus on the 7th day of pregnancy. an attempt was made on them to recover the virus from the placenta and fetuses. as a result, there were differences in the rate of placental and fetal infection among the mouse strains used. it was clarified that vertical inf ...1976185531
the 1973 epidemic of japanese encephalitis in west bengal: a serological survey of domestic animals. 1976186404
histopathological studies of brains (and other viscera) from cases of je virus encephalitis during 1973 epidemic at bankura. 1976186405
[the influence of the season of birth on preventive efficacy of the japanese encephalitis vaccine (author's transl)]. 1976190430
passive transfer of protection through immune cells in japanese encephalitis virus infection in mice--a preliminary report. 1976191393
comparative study of protection by japanese encephalitis (je) vaccine against a japanese strain and an indian strain of je virus. 1976191395
attenuation of japanese encephalitis virus by brain passage of guinea pigs.je virus was difficult to keep alive in guinea pig brain beyond 3 days and the virus was lost after 3 days. ten cross passages from guinea pig to mice attenuated je virus (gp10) and the result indicated that 3 passages could induce illness in guinea pig (gp4). gp4 and gp10 did not induce viremia to mice. 0.1 ml of 1:100,000 and 1:10,000 mouse suspension of gp4 inoculated into the author subcutaneously did not cause any uncomfortable symptom.1976192688
experimental double infection of japanese encephalitis virus and herpes simplex virus in mouse brain.in our laboratory, 64 autopsy cases of japanese encephalitis patients were examined by immunofluorescence. three patients showed some evidences of double infection of japanese encephalitis virus and herpes simplex virus. experiments were done to see the mechanisms of double infection. in doubly infected mice, japanese encephalitis virus antigen was localized in the herpes simplex virus infected areas in the brain. these results suggested that "blood brain barrier" was broken by herpes simplex vi ...1977192928
experimental studies on vertical infection of mice with japanese encephalitis virus. ii. effect of inoculation route on placental and fetal infection.studies were made on the effect of the route of inoculation on the establishment of experimental vertical infection of mice with japanese encephalitis virus. mice of the three strains, cfw, c3h/he, and c57bl/6, were inoculated with a field strain of the virus by the intravenous, intraperitoneal, or subcutaneous route. an attempt was made on them to recover the virus from the placenta and fetus. as a result, there were differences in rate of plancental and fetal infection among the route of inocu ...1977196221
[cytochalasins in virologic research]. 1977197711
an epidemiological study on the susceptibility of japanese people to japanese encephalitis virus infection by season of birth. 1977199768
intranasal infection of monkeys with japanese encephalitis virus: clinical response and treatment with a nuclease-resistant derivative of poly (i).poly (c). 1977202173
transovarial transmission of japanese encephalitis virus by mosquitoes.female aedes albopictus and aedes togoi mosquitoes infected with japanese encephalitis virus either by intrathoracic inoculation or by ingestion of a virus-sucrose-erythrocyte mixture transmitted the virus to a small percentage of their f1 progeny. adult f1 female aedes albopictus thus infected transmitted the virus in turn to newly hatched chickens by feeding on them.1978203035
[disorder of spermatogenesis and viral discharge into semen in boars infected with japanese encephalitis virus (author's transl)]. 1977203101
experimental transmission of japanese encephalitis virus through anopheles tessellatus and culex fatigans mosquitoes. 1977203526
experimental viraemia and transmission of japanese encephalitis virus by mosquitoes in ardeid birds. 1977204576
experimental viraemia and transmission of japanese encephalitis virus by mosquitoes in domestic ducks.. 1977205503
epidemiological studies on japanese encephalitis in kyoto city area, japan. i. evidence for decrease of vector mosquitoes.mosquito collections by using light traps have been carried out at 10 to 11 stations in kyoto city area at intervals of about 10 days every year. mean percent indexes (mpi), being calculated from the data of mosquito collections, were used for comparison of the annual abundance of mosquitoes. it is no doubt that culex tritaeniorhynchus summorosus has decreased recently and this decrease is correlated with the reduction of human patients of japanese encephalitis. wide use of two herbicides, cnp a ...1978207913
epidemiological studies on japanese encephalitis in kyoto city area, japan. ii. annual patterns of virus dissemination on virus recoveries from unfed culex tritaeniorhynchus summorosus.annual patterns of dissemination of japanese encephalitis virus in vector mosquitoes have been investigated at the main collection station from 1965 through 1973 at some other stations from 1969 through 1971. the virus was recovered usually from culex tritaeniorhynchus summorosus during a period of about a month from july to august every year till 1969, and from august to september after 1970, although at some of the stations the virus was recovered intermittently for longer or shorter terms. hi ...1978207914
biomedical survey in irian jaya (west irian), indonesia.a biomedical survey was conducted in several areas of irian jaya, indonesia in july 1972 in association with an investigation of reports of a cholera outbreak. stool specimens, blood smears and sera were collected and examined for evidence of parasitic as well as other infectious diseases. a total of 114 stools were examined and the most commonly found intestinal parasites were trichuris trichiura (94%), ascaris lumbricoides (74%), hookworm (58%), entamoeba coli (15%), endolimax nana (8%), entam ...1977208184
biomedical survey in the moluccas, indonesia. 1978212835
epidemiological studies on japanese encephalitis in kyoto city area, japan. iv. natural infection in sentinel pigs.natural infection with japanese encephalitis virus in three sentinel pigs held in separate experimental huts was examined daily by virus recovery from blood samples of the pigs and from mosquitoes after incubation for about 7 days from their blood feeding and by hi antibody titration of the blood samples. after a period of low infection rates, below 6%, for about two weeks in engorged culex tritaeniorhynchus summorosus, high mosquito infections of over 30% from each viremic pig occurred for two ...1978213633
transmission of japanese encephalitis virus by culex bitaeniorhynchus giles. 1978214400
an epidemiological review of japanese encephalitis.twenty-five years ago, japanese encephalitis (je) was known as an endemic, mosquito-borne disease in east asia. today, the causative virus is known to be distributed from maritime siberia to the north, eastern india to the west, the archipelago of japan presumably the philippines to the east, and from indonesia to the south. since the late-1960s, the geopathological status of je epidemics has undergone considerable changes. the sizes of je epidemics in japan and china (province of taiwan) have s ...1978214963
epidemiological studies on japanese encephalitis in kyoto city area, japan. iii. seasonal prevalence of virus infections in several pig populations shown by virus recovery from engorged culex tritaeniorhynchus summorosus.when vector mosquitoes engorged by feeding on pigs are tested for virus recovery after incubation for 7 to 10 days, the results may show mosquito infection itself. therefore, seasonal prevalence of infection in each pig population can be estimated from course of the infection rate among mosquitoes. many mosquitoes of the main vector of japanese encephalitis virus, culex tritaeniorhynchus summorosus, collected by light traps everyday or every other day in some pig sheds from 1967 to 1970 were tes ...1978215807
effects of mouse serum interferon and interferon inducers on dengue and japanese encephalitis virus infections in weanling mice. 1978216848
absence of interferon production in a newly established human cell line.a cell line established from human embryonic lung, hel-r66, was demonstrated to be highly susceptible to herpes simplex virus types 1 and 2, vaccinia virus, newcastle disease virus (ndv), japanese encephalitis virus (jev), western equine encephalitis (wee) virus, sindbis virus, vesicular stomatitis virus (vsv), and rabies virus. the maximal yields of ndv, jev, wee virus, and rabies virus in this cell line exceeded by 2--4 logs those in control human embryonic lung cells. inability of this cell l ...1979218901
japanese encephalitis virus in mosquito salivary glands.culex tritaeniorhynchus and c. pipiens mosquitoes were infected with japanese encephalitis virus either by intrathoracic injection or by membrane feeding. the virus maturation sites and the process of virus particle concentration in salivary gland cells were studied by electron microscopy. occurrence of mature virions was primarily associated with intracytoplasmic viral matrices which were extraordinarily large and had a perinuclear location in c. pipiens mosquitoes. the other sign of virus repl ...1979219722
field experiments on live attenuated japanese encephalitis virus vaccine for swine.the efficacy of a live attenuated japanese encephalitis virus (jev) vaccine was examined in swine under conditions where natural infection could occur. the pigs immunized with the vaccine produced antibodies within one week after vaccination, and the antibody was retained until the end of the experiment, i.e. 36 days. however, the antibody titers in this group were lower than that in control group naturally infected with jev. no virus was isolated from the five vaccinated pigs, but virus was iso ...1978219844
variable effect on peripheral blood leucocytes during je virus infection of man.the peripheral blood leucocytes of twenty-four cases of japanese encephalitis (je) were studied and the findings were compared with those in twenty-five normal health controls of matching age and sex. in the early phases of the disease marked neutrophil leucocytosis was seen which returned to almost normal levels by the fourth week. lymphopenia was associated with diminished t lymphocytes but the number of b lymphocytes remained within the normal range. though the number of t lymphocytes was red ...1979231492
experimental viraemia with japanese enchphalitis virus in certain domestic and peridomestic vertebrates. 1979231573
results of insecticide susceptibility tests with culex gelidus in bangalore city. 1979231574
pilot sero-epidemiological survey on japanese encephalitis in north-western burma.a pilot sero-epidemiological survey of japanese encephalitis was carried out in three areas of north-western burma applying the hemagglutination-inhibition test. the existence of japanese encephalitis infection in these areas was confirmed and its incidences in these areas were estimated and compared graphically.1979233227
arbovirus infections in sarawak, october 1968--february 1970 tembusu and sindbis virus isolations from mosquitoes.thirty isolations of tembusu virus and four of sindbis virus were obtained from approximately 280 000 mosquitoes collected between october 1968 and february 1970 in sarawak, particularly from k. tijirak, a land dyak village 19 miles south of kuching. twenty-two isolations of tembusu virus and two of sindbis virus were from culex tritaeniorhynchus; two of tembusu virus and two of sindbis virus came from culex gelidus. tembusu virus was active throughout the year at k. tijirak, the highest infecti ...1975235907
arbovirus infections in sarawak, october 1968-february 1970: getah virus isolations from mosquitoes.14 strains of getah virus were isolated from a variety of mosquito species collected in sarawak between october 1968 and february 1970. ten strains were isolated from c. tritaeniorhynchus 7 of them at k. tijirak. single strains were isolated from c. gelidus, c. pseudovishnui, m. bonneae/dives and aanopheles species. 6 of the isolates were obtained in october 1968 when japanese encephalitis, tembusu and sindbis viruses were also very active. the available evidence suggest that getah virus in sara ...1975238314
use of duck embryo cell cultures for the study of arbovirus reproduction.duck embryo cell (dec) cultures were used for the study of arbovirus reproduction. high sensitivity of dec cultures to japanese encephalitis, west nile, tyuleniy, sakhalin and baku viruses was established. dec cultures are recommended for investigation of arbovirus reproduction.1975241248
a modified method for the preparation of antigens for the indirect florescent antibody technique with japanese encephalitis virus. 1977368421
neutralization tests for dengue and japanese encephalitis viruses by the focus reduction method using peroxidase-anti-peroxidase staining.neutralization tests were made on 4 types of dengue (den) virus and japanese encephalitis (je) virus by incubation of serially diluted antisera and constant amounts of the viruses and then focus assay of surviving virus infectivity with peroxidase-anti-peroxidase (pap) staining. neutralization reactions were virtually completed in 2 hr on incubation of serum-virus mixtures at 28 c. a straight regression line was obtained on a probit chart by plotting the focus reduction rates at various dilution ...1978383069
morphological properties of japanese encephalitis virus; its nucleocapsid. 1979440145
geographic distribution of arbovirus antibodies in indigenous human populations in the indo-australian archipelago.sera from lifetime residents in 16 localities of the indo-australian archipelago and adjacent areas were tested for hemagglutination-inhibition antibody against four alpha-viruses (sindbis, getah, chikungunya, and ross river) and for neutralizing antibody against six flaviviruses (dengue 2 and 3, japanese encephalitis, murray valley encephalitis, kunjin, and edge hill). mosquito collections were carried out in some of the localities to study vector relationships to distribution of the arbovirus ...1979453438
proteins specified by togaviruses in infected aedes albopictus (singh) mosquito cells.yields of greater than 10(7) p.f.u./ml at 28 or 37 degrees c of the alphavirus sindbis and the flavivirus kunjin were obtained in the aedes albopictus (singh) cell line, the latent periods being 4 to 6 and 10 to 12 h, respectively. despite a high background of host protein synthesis, virtually all the virus-specified proteins of the flaviviruses kunjin, dengue-2 and japanese encephalitis were labelled and resolved by slab gel electrophoresis of infected and uninfected cell proteins. in contrast, ...1979479844
accelerated degradation tests on some immunological products. 1979489620
intracellular distribution of virus-specific rna in chick embryo cells infected with japanese encephalitis virus.japanese encephalitis virus (jev) infected chick embryo (ce) cells were treated with 4 mug actinomycin d/ml and 5 mm-d-glucosamine at 2 or 3 h before harvesting. production of jev was not affected by the short-time treatment of these drugs. the radioactivity in virus-specific rna in the glucosamine-treated cells was a-parently higher than in non-treated cells. nuclear and cytoplasmic extracts were prepared from the jev-infected cells pulse-labelled with 3h-uridine at 15 h after infection. analys ...1977556758
polykaryocytosis induced by certain arboviruses in monolayers of bhk-21-528 cells.multinucleated giant cell formation in a clone of bhk-21 cells, bhk-21-528, was tested with certain arboviruses. eleven out of 19 viruses tested, chikungunya, getah, sagiyama, sindbis, western equine encephalitis, st. louis encephalitis, japanese encephalitis, west nile, bunyamwera, germiston and california encephalitis virus induced cell fusion after infection. all cases where giant cells were observed during the experiments, involved the b type cell fusion (fusion from within).1977557074
association of virus specific replicative ribonucleic acid with nuclear membrane in chick embryo cells infected with japanese encephalitis virus.using high resolution electron microscopic autoradiography and velocity sedimentation, rna synthesis was examined in chick embryo cells infected with japanese encephalitis virus (jev). rna was labelled with 3h-uridine for 5 min or 10 min at 15 h after infection in the presence of actinomycin d and d-glucosamine. microautoradiography showed significantly numbers of silver grains on the nuclear membranes of 5 min pulse-labelled thin cell sections. the rna species in membrane fractions obtained fro ...1978564392
critical evaluation of the role of immunization as an etiological factor of infantile spasms.one hundred and ten cases of infantile spasms with detailed information about immunization available were selected as the masterial to evaluate the significance of immunization as an etiological factor of infantile spasms. in 80% of the cases, immunization could not be considered to have any relation with causation of infantile spasms, because 44 cases (40%) never had innoculations as yet, and other 44 cases (40%) had been immunized by some vaccines over one month before or after the onset of th ...1977578294
electron microscopic observation of the budding maturation of group b arboviruses. 1977593173
[characterization of the attenuated os- strain of japanese encephalitis virus (author's transl)]. 1977602024
ultrastructure of soluble complement fixing antigen and antibody of japanese encephalitis virus. 1977608702
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