| rice black-streaked dwarf virus: its properties, morphology and intracellular localization. | | 1977 | 855190 |
| conserved terminal nucleotide sequences in the genome of rice black streaked dwarf virus. | the terminal regions of the dsrna genome segments of rice black streaked dwarf virus (rbsdv) were sequenced. the individual dsrnas, which were 32p-labelled at their 3' termini by incubation with [32p]pcp and t4rna ligase, were separated by 5% page, and the 10 dsrna segments were sequenced by two-dimensional electrophoresis. the common 3'-terminal sequences ---guc 3' and ---aaaaacuu 3' were found in the plus and minus strands, respectively. the strictly conserved terminal sequences (5' aaguuuuu . ... | 1992 | 1607875 |
| serological relationship between maize rough dwarf virus and rice black-streaked dwarf virus. | | 1973 | 4215213 |
| nucleotide sequence of nilaparvata lugens reovirus genome segment s8 coding for the major outer capsid protein. | the complete nucleotide sequence of genome segment 8 (s8) of nilaparvata lugens reovirus (nlrv) was determined. it consisted of 1802 nucleotides containing a long open reading frame (562 amino acids), which was expressed in escherichia coli as a fusion protein. the expressed s8 product, a 62k protein, was detected by western blotting using igg directed against intact nlrv particles. this result indicates that s8 encodes the major outer capsid protein of nlrv. the protein exhibited 18.6% amino ac ... | 1994 | 7931170 |
| close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. | the complete nucleotide sequences of rice black-streaked dwarf virus (rbsdv) genome segments 8 (s8) and 7 (s7) were determined, and were found to have high sequence identities to the corresponding maize rough dwarf virus (mrdv) genome segments. rbsdv s8 and s7 consisted of 1927 and 2193 nucleotides, respectively. rbsdv s8 had a single long open reading frame (orf) encoding 591 amino acids. rbsdv s7 had two nonoverlapping orfs encoding 362 (orf1) and 309 (orf2) amino acids. the two orfs of rbsdv ... | 1993 | 8336114 |
| complete nucleotide sequence of the nilaparvata lugens reovirus: a putative member of the genus fijivirus. | the nucleotide sequences of all genome segments of the nilaparvata lugens reovirus (nlrv), which is found in the brown planthopper nilaparvata lugens, have been determined and some genes have been assigned to structural and functional proteins. the genome of nlrv consists of 28 699 nucleotides and contains at least 11 large open reading frames (orfs). the genome of nlrv is the largest among viruses of the family reoviridae reported to date. the deduced amino acid sequence of genome segment s1 co ... | 1996 | 8558122 |
| taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome. | sequence determination of full-length cdna clones of genomic segments 7-10 (s7-s10) of oat sterile dwarf fijivirus (osdv) revealed that the 5' and 3' ends of the plus strands of these segments had the same conserved terminal sequences, 5' aacgaaaaa and uuuuuuuaguc 3'. these sequences are similar, but not identical, to the conserved terminal nucleotide sequences of the genomic segments of rice black streaked dwarf fijivirus (rbsdv) and maize rough dwarf fijivirus (mrdv). the coding strands of s7 ... | 1998 | 9634091 |
| identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in china. | three virus isolates from maize with rough dwarf in different provinces in china were analyzed at the molecular level. when compared to an isolate from diseased rice plants in hubei province, all four isolates had identical genomic rna electrophoretic profiles, which were composed of ten double-stranded (ds) rnas. full-length cdnas of segment 10 (s10) from each of the four isolates were cloned by rt-pcr and the complete sequences were determined. analysis of the sequences revealed that each cons ... | 2001 | 11266211 |
| molecular characterisation of segments 1 to 6 of rice black-streaked dwarf virus from china provides the complete genome. | the nucleotide sequences of segments s1 to s6 of a chinese isolate of rice black-streaked dwarf virus (rbsdv) were determined. this provides the first complete sequence of a plant pathogenic member of the genus fijivirus. the complete ten-segment genome has 29,141 nucleotides, making it the largest reovirus genome so far reported. each of the segments s1-s6 is predicted to encode a single major protein. protein comparisons indicated that s1 encoded an rna dependent rna polymerase, with similarit ... | 2001 | 11811683 |
| cloning and sequencing of the genome segment s9 of rice black-streaked dwarf virus. | genome segment s9's of rice black-streaked dwarf virus (rbsdv) of three chinese isolates were amplified by rt-pcr and sequenced, and were found to be consisted of 1 900 nt (rbsdv-zj s9 embl accession number aj297430),1 898 nt (rbsdv-heb s9 embl accession number aj297429) and 1 900 nt (rbsdv-hub s9 embl accession number aj291706), respectively. genome segment s9's of three chinese isolates shared 98.5%--98.8% sequence homology and all contained two open reading frames (orf), which encoded two pol ... | 2001 | 12040425 |
| identification of rice black streaked dwarf virus in different cereal crops with dwarfing symptoms in china. | this report describes isolation of virus particles from plants of rice, maize, wheat and sorghum with symptoms of dwarfing collected from two provinces of china, purification of double-stranded rna (dsrna) from the virus particles, and synthesis of full-length cdnas of genome segments 9 (s9) and 10 (s10) by reverse transcription-polymerase chain reaction (rt-pcr). sequence analysis showed that the s9 sequences of the chinese isolates and a japanese rice black-streaked disease virus (rbsdv) isola ... | 2001 | 12083334 |
| nucleotide sequences of double-stranded rna segments from a hypovirulent strain of the white root rot fungus rosellinia necatrix: possibility of the first member of the reoviridae from fungus. | twelve double-stranded (ds) rna segments were detected from a hypovirulent strain w370 of the white root rot fungus rosellinia necatrix. the estimated molecular weights ranged from 0.41 x 10(6) to 2.95 x 10(6). full length cdna clones for eight segments were obtained. northern blot analysis suggested that each segment was genetically unique. the nucleotide sequences of eight full length dsrna segments were determined. one long open reading frame was found in each segment. conserved sequences at ... | 2002 | 12206302 |
| sequence analysis of genome segments s4 and s8 of mal de río cuarto virus (mrcv): evidence that the virus should be a separate fijivirus species. | this is the first sequence-based characterization of mal de río cuarto virus (mrcv), currently classified as a variant of maize rough dwarf virus (mrdv) and exclusively found in south america. we sequenced and analyzed genome segments s4 and s8. mrcv s4 coded for a putative 131.67 kda protein while mrcv s8 coded for a putative 68.26 kda protein containing an atp/gtp-binding motif. the 5' and 3' ends of mrcv segments, were 5'aaguuuuu3' and 5'cagcunnnguc3', respectively. prediction of secondary st ... | 2002 | 12209310 |
| selection of phage-display peptides that bind specifically to the outer coat protein of rice black streaked dwarf virus. | several peptides that could bind specifically to the outer coat protein encoded by the s10 gene of rice black streaked virus (rbsdv) were isolated from a phage-display random 12-mer peptide library. the sequence analysis showed that the amino acid motif (k)k**(*)p, the asterisk denoting any amino acid, might be the core sequence by which the peptides bind to the target protein. the peptide 1 that had a high affinity to rbsdv outer coat protein was synthesized by a chemical method and its fusion ... | 2002 | 12387499 |
| phylogenetic analysis reveals that a dwarfing disease on different cereal crops in china is due to rice black streaked dwarf virus (rbsdv). | a viral disease with dwarfing symptoms is associated with severe damage of different cereal crops including rice, maize, wheat and sorghum grown in china. it is believed that the pathogenic agent of the disease on rice and sorghum is rice black streaked dwarf virus (rbsdv), however, the cause of maize dwarf disease in china is still inconclusive. in this report, dsrna was isolated from virus particles obtained from the diseased plants of rice, maize, wheat and sorghum from two chinese provinces. ... | 2002 | 12416683 |
| [cdna cloning and sequence analysis of genome segment s7 of rice black-streaked dwarf virus]. | genome segments 7 of zhejiang and hebei isolates of rice black-streaked dwarf virus (rbsdv) were amplified and sequenced. segment 7 of zhejiang isolate was consisted of 2193 nts (embl accession no. aj297427) in length and that of hebei isolate was 2190 nts (aj297428). both segments contained two non-overlapping open reading frames (orfs), which encoded two polypeptides with molecular weights of 41 kd and 36 kd. these two segments shared 99% nucleotide identity, 100% and 94.4% amino acid identiti ... | 2002 | 12557397 |
| sequence and phylogenetic analysis of genome segments s1, s2, s3 and s6 of mal de río cuarto virus, a newly accepted fijivirus species. | mal de río cuarto virus (mrcv) is a newly described species of the genus fijivirus, family reoviridae. the nucleotide sequence of four mrcv genome segments was determined. mrcv s1, s2, s3 and s6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kda, respectively. mrcv s1 encodes a basic protein that contains conserved rna-dependent rna polymerase motifs, and is homologous to rice black streaked dwarf virus (rbsdv), fiji disease virus (fdv) and nilaparvata lugens reovirus (nlrv) pol ... | 2003 | 12606083 |
| molecular analysis of fiji disease fijivirus genome segments 1 and 3. | fiji disease fijivirus (fdv) genomic segments 1 (s1) and 3 (s3) were completely sequenced. fdv s1 comprised 4,532 nt and was predicted to encode a 170.6 kda protein. fdv s3 comprised 3,623 nt and was predicted to encode a 135.5 kda protein. the terminal sequences of s1 and s3 were 5' aaguuuuu......cagcuagcguc 3' and 5' aaguuuuu......cagcagauguc 3', respectively, and located immediately adjacent to these sequences were 12 bp imperfect inverted repeats. the predicted translation product of fdv s1 ... | 2003 | 12876456 |
| sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. | the complete nucleotide sequences of 10 genomic segments (s1-s10) from an isolate of rice black-streaked dwarf virus causing rough dwarf disease on maize (rbsdv-hbm) in china were determined, a total of 29,142 base pairs (bp). each segment possessed the genus-specific termini with conserved nucleotide sequences of (+) 5'-aaguuuuu......cagcunnnguc-3' and a perfect or imperfect inverted repeat of seven to eleven nucleotides immediately adjacent to the terminal conserved sequence. while the coding ... | 2003 | 14501194 |
| molecular analysis of fiji disease virus genome segments 5, 6, 8 and 10. | the complete sequences of fiji disease virus (fdv) genome segments 5 (s5), s6, s8 and s10 were obtained and comprised 3150 nt, 2831 nt, 1959 nt and 1819 nt, respectively. each segment contained a single orf which encoded putative proteins of 115 kda, 97 kda, 69 kda and 63.0 kda, respectively. the putative amino acid sequences encoded by s5 and s6 contained putative leucine zipper motifs while fdv s5 and s8 each contained an atp-gtp-binding motif. at the amino acid level, fdv s5, s6, s8 and s10 s ... | 2004 | 15045559 |
| sequence analysis of genome segments s5 and s10 of mal de rio cuarto virus (fijivirus, reoviridae). | mal de rio cuarto virus (mrcv) was recently described as a new species of the genus fijivirus, family reoviridae. the nucleotide sequence of two mrcv genome segments was determined. mrcv s5 and s10 were predicted to encode proteins of 106.9 and 63.5 kda respectively. the protein coded by mrcv s5 had 62.8% and 35.7% identity to fijiviruses rbsdv s5 and fdv s5 coded proteins, and contained a rarely reported type-1 c-terminal peroxisomal targeting signal. the protein coded by mrcv s10 had identity ... | 2005 | 15747051 |
| [cdna cloning and expression in escherichia coli of rice black-streaked dwarf virus segment 7]. | using primers designed from the terminal sequences of maize rough dwarf virus s6, a 2.2 kb cdna fragment was amplified by rt-pcr from maize plants showing maize rough dwarf disease. sequence analysis shows that the full length of this cdna is 2193bp. it contains two open reading frames that encoded two polypeptides with molecular weight of 41.0kd and 36.3kd, respectively. results of multi-sequences alignment suggest that, this cdna sequence has significant similarity to rice black-streaked dwarf ... | 2003 | 16276917 |
| [cloning and expression of s9-1 gene of rice black-streaked dwarf virus in escherichia coli]. | the cdna of rbsdv s9 was amplified by rt-pcr using the total rna of infected maize leaves as the template, and then the pcr product was cloned into pbluescriptii sk. after the dna sequence was determined, the s9-1 gene was subcloned into e. coli expression vector pet-21d. sds-page analysis revealed that a 40 kd protein was highly expressed. n-terminal sequence analysis confirmed the 40 kd protein was s9-1 protein. antiserum was prepared using the purified protein. western blotting indicated that ... | 2003 | 16279198 |
| development of an id-elisa for the detection of rice black-streaked dwarf virus in plants. | the rice black-streaked dwarf virus (rbsdv) virion is composed of two layers of capsid proteins and 10 segments of double-stranded genomic rna (s1-s10). due to the fragility of the rbsdv outer capsid, it is very difficult to obtain intact virus particles for preparation of antiserum needed in virus detection. in this work, the major outer capsid protein (p10) encoded by s10 of rbsdv was expressed in escherichia coli cells as a glutathione-s-transferase (gst) fusion protein. after purification of ... | 2006 | 16406134 |
| molecular analysis of fiji disease virus segments 2, 4 and 7 completes the genome sequence. | the complete nucleotide sequences of fiji disease virus (fdv) genome segments s2, s4 and s7 were determined. this now completes the sequencing of all ten dsrna genome segments of the fijivirus type member, fdv, which comprises a total of 29339 nt. fdv s2, s4 and s7 comprised 3820, 3568 and 2194 nt, respectively. s2 and s4 each contained a single open reading frame (orf), which encoded putative proteins of 137 and 133 kda, respectively, while s7 contained two orfs, which encoded putative proteins ... | 2006 | 16525734 |
| sequencing of the bicistronic genome segments s7 and s9 of mal de río cuarto virus (fijivirus, reoviridae) completes the genome of this virus. | the nucleotide sequences of genomic segments s7 and s9 of mal de río cuarto virus (mrcv, fijivirus group ii) have been determined, thus completing the entire genome sequence of the virus. these segments showed a non-overlapping bicistronic structure, as in other members of the genus. mrcv s7 orf-1 had a length of 1086 bp and encoded a 41.5 kda putative polypeptide, whereas mrcv s7 orf-2 had a length of 930 bp and encoded a 36.8 kda putative polypeptide. proteins of 39 and 20.5 kda were predicted ... | 2007 | 17115303 |
| rice black-streaked dwarf virus outer capsid protein p10 has self-interactions and forms oligomeric complexes in solution. | the p10 protein encoded by s10 orf of rice black-streaked dwarf virus (rbsdv) was thought to be the component of outer shell of viral particle. in the present study, p10 has an ability for self-interaction as shown by a gal4 transcription activator-based yeast two-hybrid assay system and further confirmed by in vitro far-western blot analysis. the domain responsible for p10-p10 self-interaction was mapped to the first 230 amino acids at the n-terminal region of the protein. the oligomerization p ... | 2007 | 17442443 |
| rice black-streaked dwarf virus minor core protein p8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts. | virus-encoding nuclear transcriptional regulators play important roles in the viral life cycle. most of these proteins exhibit intrinsic transcriptional activation or repression activity, and are involved in the regulation of the expression of virus genome itself or important cellular genes to facilitate viral replication and inhibit antiviral responses. here, we report that the minor core protein p8 of rice black-streaked dwarf virus, a dsrna virus infecting host plants and insects, is targeted ... | 2007 | 17499245 |
| rice black streaked dwarf virus p9-1, an alpha-helical protein, self-interacts and forms viroplasms in vivo. | replication and assembly of viruses from the family reoviridae are thought to take place in discrete cytoplasmic inclusion bodies, commonly called viral factories or viroplasms. rice black streaked dwarf virus (rbsdv) p9-1, a non-structural protein, has been confirmed to accumulate in these intracellular viroplasms in infected plants and insects. however, little is known about its exact function. in this study, p9-1 of rbsdv-baoding was expressed in escherichia coli as a his-tagged fusion protei ... | 2008 | 18559948 |
| a black-streaked dwarf disease on rice in china is caused by a novel fijivirus. | an isolate of a plant reovirus causing severe stunting and dark leaf symptoms on rice from guangdong, china, was similar in virion morphology and serologically related to rice black-streaked dwarf virus (rbsdv). the electrophoretic profiles of genome segments of the two viruses in agarose or polyacrylamide gel were indistinguishable. the four genome segments of the new isolate corresponding to rbsdv s7-s10 were amplified by ligation rt-pcr and sequenced. the size and organization of each genome ... | 2008 | 18820828 |
| molecular detection of nine rice viruses by a reverse-transcription loop-mediated isothermal amplification assay. | a reverse-transcription loop-mediated isothermal amplification (rt-lamp) assay was established for the detection of nine viruses from infected rice plants, including rice black-streaked dwarf virus (rbsdv), rice dwarf virus (rdv), rice gall dwarf virus (rgdv), rice ragged stunt virus (rrsv), rice transitory yellowing virus (rtyv), rice stripe virus (rsv), rice grassy stunt virus (rgsv), rice tungro spherical virus (rtsv), and rice tungro bacilliform virus (rtbv). virus-specific primer sets were ... | 2010 | 20837064 |
| rice black-streaked dwarf virus p6 self-interacts to form punctate, viroplasm-like structures in the cytoplasm and recruits viroplasm-associated protein p9-1. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus within the family reoviridae, can infect several graminaceous plant species including rice, maize and wheat, and is transmitted by planthoppers. although several rbsdv proteins have been studied in detail, functions of the nonstructural protein p6 are still largely unknown. | 2011 | 21241517 |
| molecular characterization of segments s7 to s10 of a southern rice black-streaked dwarf virus isolate from maize in northern china. | southern rice black-streaked dwarf virus (srbsdv) is a novel fijivirus prevalent in rice in southern and central china, and northern vietnam. its genome has 10 segments of double-stranded rna named s1 to s10 according to their size. an isolate of srbsdv, jni4, was obtained from naturally infected maize plants from ji'ning, shandong province, in the 2008 maize season. segments s7 to s10 of jni4 share nucleotide identities of 72.6%-73.1%, 72.3%-73%, 73.9%-74.5% and 77.3%-79%, respectively, with co ... | 2011 | 21331890 |
| immunity to rice black streaked dwarf virus, a plant reovirus, can be achieved in rice plants by rna silencing against the gene for the viroplasm matrix protein. | the nonstructural protein p9-1 of rice black streaked dwarf virus has been confirmed to accumulate in viroplasms, the putative sites of viral replication, in infected plants and insects. we transformed rice plants by introducing an rna interference construct against the p9-1-encoding gene. the resultant transgenic plants accumulated short interfering rnas specific to the construct. all progenies produced by self-fertilization of these transgenic plants with induced rna interference against the g ... | 2011 | 21621568 |
| the p7-1 protein of southern rice black-streaked dwarf virus, a fijivirus, induces the formation of tubular structures in insect cells. | southern rice black-streaked dwarf virus (srbsdv), an insect- and plant-infecting reovirus of the genus fijivirus, induced the formation of virus-containing tubules in infected plant and insect vector cells. expression of the nonstructural protein p7-1 of srbsdv in insect cells by a recombinant baculovirus resulted in the formation of tubules with dimensions and appearance similar to those found in srbsdv-infected cells. these tubules protruded from the cell surface, supporting the hypothesis th ... | 2011 | 21671041 |
| proteome profile of maize (zea mays l.) leaf tissue at the flowering stage after long-term adjustment to rice black-streaked dwarf virus infection. | maize rough dwarf disease (mrdd) is a viral disease and causes great yield loss. to better understand the effects of mrdd on plant growth and metabolism, comparative proteomic analysis of leaves from virus-infected and normal plants was performed. in order to eliminate the interference of ribulose-1, 5-bisphosphate carboxylase with low-abundance proteins, total proteins were pre-fractionated by 15% peg and the proteins from supernatant and precipitated fractions were analyzed by 2-de, subsequent ... | 2011 | 21708230 |
| Alteration of gene expression profile in maize infected with a double-stranded RNA fijivirus associated with symptom development. | Maize rough dwarf disease caused by Rice black-streaked dwarf virus (RBSDV) is a major viral disease in China. It has been suggested that the viral infection of plants might cause distinct disease symptoms through the inhibition or activation of host gene transcription. We scanned the gene expression profile of RBSDV-infected maize through oligomer-based microarrays to reveal possible expression changes associated with symptom development. Our results demonstrate that various resistance-related ... | 2011 | 21955602 |
| crystallographic analysis reveals octamerization of viroplasm matrix protein p9-1 of rice black streaked dwarf virus. | the p9-1 protein of rice black streaked dwarf virus accumulates in viroplasm inclusions, which are structures that appear to play an important role in viral morphogenesis and are commonly found in viruses in the family reoviridae. crystallographic analysis of p9-1 revealed structural features that allow the protein to form dimers via hydrophobic interactions. each dimer has carboxy-terminal regions, resembling arms, that extend to neighboring dimers, thereby uniting sets of four dimers via later ... | 2012 | 22072761 |
| reverse transcription loop-mediated isothermal amplification of rna for sensitive and rapid detection of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) causes one of the most serious viral diseases of rice in southeast asia. a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for detection of srbsdv using total rna extracted from rice tissues and the insect pest, white-backed planthopper. the assay was based on a set of four primers matching a total of six sequences in the s9 region of srbsdv genome. presence of the virus could be detected in rt-lamp reac ... | 2011 | 22227615 |
| expression of cytoplasmic incompatibility and host fitness effects in field populations of sogatella furcifera infected with cardinium. | 'candidatus cardinium' is a maternally inherited intracellular bacterium that infects a wide range of arthropods and causes diverse reproductive alterations of its arthropod hosts, including cytoplasmic incompatibility (ci), parthenogenesis and feminization. ci is the most common effect and is expressed as a reduction in the number of offspring in crosses between infected males and uninfected females (or females infected with a different bacterial strain). the white-backed planthopper sogatella ... | 2012 | 23356082 |
| the secretory pathway and the actomyosin motility system are required for plasmodesmatal localization of the p7-1 of rice black-streaked dwarf virus. | rice black-streaked dwarf virus (rbsdv), a plant-infecting reovirus (genus fijivirus), generally induces virus-containing tubules in infected cells. the nonstructural protein p7-1, encoded by the first open reading frame of segment 7, is involved in forming the structural matrix of these tubules. in experiments to investigate the subcellular localization of p7-1 in nicotiana benthamiana epidermal cells, fluorescence of p7-1:egfp was observed in the nucleus, cytoplasm and cell periphery, and in p ... | 2012 | 23271163 |
| small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus. | numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ... | 2015 | 26537672 |
| identification and profiling of conserved and novel micrornas in laodelphax striatellus in response to rice black-streaked dwarf virus (rbsdv) infection. | micrornas (mirnas) are small non-coding endogenous rna molecules that play important roles in various biological processes. this study examined microrna profiles of laodelphax striatellus using the small rna libraries derived from virus free (vf) and rice black-streaked dwarf virus (rbsdv) infected (rb) insects. a total of 59 mature mirnas (46 mirna families) were identified as conserved insect mirnas in both vf and rb libraries. among these conserved mirnas, 24 were derived from the two arms of ... | 2014 | 26484150 |
| transcriptome and comparative gene expression analysis of sogatella furcifera (horváth) in response to southern rice black-streaked dwarf virus. | the white backed planthopper (wbph), sogatella furcifera (horváth), causes great damage to many crops by direct feeding or transmitting plant viruses. southern rice black-streaked dwarf virus (srbsdv), transmitted by wbph, has become a great threat to rice production in east asia. | 2012 | 22558400 |
| understanding the immune system architecture and transcriptome responses to southern rice black-streaked dwarf virus in sogatella furcifera. | sogatella furcifera, the white-backed planthopper (wbph), has become one of the most destructive pests in rice production owing to its plant sap-sucking behavior and efficient transmission of southern rice black-streaked dwarf virus (srbsdv) in a circulative, propagative and persistent manner. the dynamic and complex srbsdv-wbph-rice plant interaction is still poorly understood. in this study, based on a homology-based genome-wide analysis, 348 immune-related genes belonging to 28 families were ... | 2016 | 27805032 |
| analysis of sogatella furcifera proteome that interact with p10 protein of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv) is transmitted efficiently only by white-backed planthopper (wbph, sogatella furcifera) in a persistent propagative manner. here we used a yeast two-hybrid system to investigate the interactions between the srbsdv- p10 and the cdna library of wbph. of 130 proteins identified as putative interactors, 28 were further tested in a retransformation analysis and β-galactosidase assay to confirm the interaction. the full-length gene sequences of 5 candi ... | 2016 | 27653366 |
| maize-pathogen interactions: an ongoing combat from a proteomics perspective. | maize (zea mays l.) is a host to numerous pathogenic species that impose serious diseases to its ear and foliage, negatively affecting the yield and the quality of the maize crop. a considerable amount of research has been carried out to elucidate mechanisms of maize-pathogen interactions with a major goal to identify defense-associated proteins. in this review, we summarize interactions of maize with its agriculturally important pathogens that were assessed at the proteome level. employing diff ... | 2015 | 26633370 |
| selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time pcr. | both genome-wide transcriptomic surveys of the mrna expression profiles and virus-induced gene silencing-based molecular studies of target gene during virus-plant interaction involve the precise estimation of the transcript abundance. quantitative real-time pcr (qpcr) is the most widely adopted technique for mrna quantification. in order to obtain reliable quantification of transcripts, identification of the best reference genes forms the basis of the preliminary work. nevertheless, the stabilit ... | 2013 | 23954326 |
| simultaneous detection and differentiation of rice black streaked dwarf virus (rbsdv) and southern rice black streaked dwarf virus (srbsdv) by duplex real time rt-pcr. | the diseases caused by rice black streaked dwarf virus (rbsdv) and southern rice black streaked dwarf virus (srbsdv) have been occurring epidemically in china and southeastern asia in recent years. a sensitive, reliable and quantitative method is required to detect and distinguish for rbsdv and srbsdv in rice and vector insects. | 2013 | 23331990 |
| enhanced virus resistance in transgenic maize expressing a dsrna-specific endoribonuclease gene from e. coli. | maize rough dwarf disease (mrdd), caused by several fijiviruses in the family reoviridae, is a global disease that is responsible for substantial yield losses in maize. although some maize germplasm have low levels of polygenic resistance to mrdd, highly resistant cultivated varieties are not available for agronomic field production in china. in this work, we have generated transgenic maize lines that constitutively express rnc70, a mutant e. coli dsrna-specific endoribonuclease gene. transgenic ... | 2013 | 23593318 |
| identification and fine-mapping of a qtl, qmrdd1, that confers recessive resistance to maize rough dwarf disease. | maize rough dwarf disease (mrdd) is a devastating viral disease that results in considerable yield losses worldwide. three major strains of virus cause mrdd, including maize rough dwarf virus in europe, mal de río cuarto virus in south america, and rice black-streaked dwarf virus in east asia. these viral pathogens belong to the genus fijivirus in the family reoviridae. resistance against mrdd is a complex trait that involves a number of quantitative trait loci (qtl). the primary approach used t ... | 2013 | 24079304 |
| structure and components of the globular and filamentous viroplasms induced by rice black-streaked dwarf virus. | viroplasms of members of the family reoviridae are considered to be viral factories for genome replication and virion assembly. globular and filamentous phenotypes have different components and probably have different functions. we used transmission electron microscopy and electron tomography to examine the structure and components of the two viroplasm phenotypes induced by rice black-streaked dwarf virus (rbsdv). immuno-gold labeling was used to localize each of the 13 rbsdv encoded proteins as ... | 2017 | 28359957 |
| the influence of sogatella furcifera (hemiptera: delphacidae) migratory events on the southern rice black-streaked dwarf virus epidemics. | southern rice black-streaked dwarf virus (srbsdv) caused serious rice losses. after the first outbreak in 2009 in northern vietnam and southern china, the virus ravaged crops again on enormous scales in 2010, but infections have decreased sharply since 2011. we presumed that the sudden epidemics and fadeout of srbsdv would be closely related to the migratory events of the insect vector, sogatella furcifera. this study sought the source area of srbsdv using the trajectory analysis method, and rev ... | 2017 | 28334380 |
| evaluation of rice resistance to southern rice black-streaked dwarf virus and rice ragged stunt virus through combined field tests, quantitative real-time pcr, and proteome analysis. | diseases caused by southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) considerably decrease grain yield. therefore, determining rice cultivars with high resistance to srbsdv and rrsv is necessary. in this study, rice cultivars with high resistance to srbsdv and rrsv were evaluated through field trials in shidian and mangshi county, yunnan province, china. sybr green i-based quantitative real-time polymerase chain reaction (qrt-pcr) analysis was used to quantitat ... | 2017 | 28241456 |
| characteristics of sirnas derived from southern rice black-streaked dwarf virus in infected rice and their potential role in host gene regulation. | virus-derived sirnas (vsirnas)-mediated rna silencing plays important roles in interaction between plant viruses and their hosts. southern rice black-streaked dwarf virus (srbsdv) is a newly emerged devastating rice reovirus with ten dsrna genomic segments. the characteristics of srbsdv-derived sirnas and their biological implications in srbsdv-rice interaction remain unexplored. | 2017 | 28183327 |
| interaction between southern rice black-streaked dwarf virus minor core protein p8 and a rice zinc finger transcription factor. | the fijivirus southern rice black-streaked dwarf virus (srbsdv) causes one of the most serious viral diseases of rice in china and vietnam. to better understand the molecular basis of srbsdv infection, a yeast two-hybrid screen of a rice cdna library was carried out using p8, a minor core protein of srbsdv, as the bait. a rice cys2his2-type zinc finger protein (oszfp) was found to interact with srbsdv p8. a strong interaction between srbsdv p8 and oszfp was then confirmed by pull-down assays, an ... | 2017 | 28124144 |
| jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to rice black streaked dwarf virus infection in rice. | plant hormones play a vital role in plant immune responses. however, in contrast to the relative wealth of information on hormone-mediated immunity in dicot plants, little information is available on monocot-virus defense systems. we used a high-throughput-sequencing approach to compare the global gene expression of rice black-streaked dwarf virus (rbsdv)-infected rice plants with that of healthy plants. exogenous hormone applications and transgenic rice were used to test rbsdv infectivity and p ... | 2017 | 27976810 |
| virus altered rice attractiveness to planthoppers is mediated by volatiles and related to virus titre and expression of defence and volatile-biosynthesis genes. | viruses may induce changes in plant hosts and vectors to enhance their transmission. the white-backed planthopper (wbph) and brown planthopper (bph) are vectors of southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv), respectively, which cause serious rice diseases. we herein describe the effects of srbsdv and rrsv infections on host-selection behaviour of vector and non-vector planthoppers at different disease stages. the y-tube olfactometer choice and free-choic ... | 2016 | 27924841 |
| rnai-mediated resistance to rice black-streaked dwarf virus in transgenic rice. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to rbsdv have not yet been found. one of the most promising methods to confer resistance against rbsdv is the use of rna interference (rnai) technology. rbsdv non-structural pro ... | 2017 | 27900537 |
| characterization of small interfering rnas derived from rice black streaked dwarf virus in infected maize plants by deep sequencing. | rice black streaked dwarf virus (rbsdv) is the casual agent of maize rough dwarf disease, which frequently causes severe yield loss in china. however, the interaction between rbsdv and maize plants is largely unknown. rna silencing is a conserved mechanism against viruses in plants. to understand the antiviral rna interfering response in rbsdv-infected plants, the profile of virus-derived small interfering rnas (vsirnas) from rbsdv in infected maize plants was obtained by deep sequencing in this ... | 2017 | 27888127 |
| the speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors. | rice black-streaked dwarf virus (rbsdv) and southern rice black-streaked dwarf virus (srbsdv) are two closely related fijiviruses transmitted by the small brown planthopper (sbph) and white-backed planthopper (wbph), respectively. srbsdv has a latent period 4 days shorter than that of rbsdv, implying a more efficient spread in insect vector. currently, the mechanisms underlying this higher efficiency are poorly understood. however, our recent studies have implicated a role of virus induced tubul ... | 2016 | 27760544 |
| asymmetric spread of srbsdv between rice and corn plants by the vector sogatella furcifera (hemiptera: delphacidae). | plant viruses are mostly transmitted by sucking insects via their piercing behaviors, which may differ due to host plant species and their developmental stages. we characterized the transmission of a fijivirus, southern rice black-streaked dwarf virus (srbsdv), by the planthopper vector sogatella furcifera horváth (hemiptera: delphacidae), between rice and corn plants of varying developmental stages. srbsdv was transmitted from infected rice to uninfected corn plants as efficiently as its transm ... | 2016 | 27760223 |
| molecular variation and expansion of a rice black-streaked dwarf virus population based on analysis of segment 1 in jining, china. | to analyze the variation in rice black-streaked dwarf virus (rbsdv) in an area with high incidence of maize rough dwarf disease (mrdd), the rbsdv s1 segment in a collection of 100 maize isolates (sample population a100) from jining, shandong province, was sequenced. an additional 21 maize and rice isolates (subpopulation b21) that were sampled from nine other geographic locations in china in 2012 and 2013 were used as a control. a total of 914 nucleotide mutations, including 239 singleton variab ... | 2016 | 27638775 |
| tolerance and responsive gene expression of sogatella furcifera under extreme temperature stresses are altered by its vectored plant virus. | southern rice black-streaked dwarf virus (srbsdv), a newly emerged fijivirus causing great loss to rice production in eastern and southeastern asian countries in recent years, is efficiently transmitted by a rice pest, white-backed planthopper (wbph, sogatella furcifera) in a persistent, circulative propagative manner and can be considered as an insect virus. in this study, srbsdv infection in wbph was found to increase the vector's death rate under extreme cold stress but improve its survival r ... | 2016 | 27531640 |
| dual transcriptome analysis reveals insights into the response to rice black-streaked dwarf virus in maize. | maize rough dwarf disease (mrdd) is a viral infection that results in heavy yield losses in maize worldwide, particularly in the summer maize-growing regions of china. mrdd is caused by the rice black-streaked dwarf virus (rbsdv). in the present study, analyses of micrornas (mirnas), the degradome, and transcriptome sequences were used to elucidate the rbsdv-responsive pathway(s) in maize. genomic analysis indicated that the expression of three non-conserved and 28 conserved mirnas, representing ... | 2016 | 27493226 |
| epg recordings reveal differential feeding behaviors in sogatella furcifera in response to plant virus infection and transmission success. | plant viruses are primarily transmitted by insect vectors and virus infection may influence on the vectors' feeding behaviors. using an electrical penetration graph, we detected that infection with the southern rice black-streaked dwarf virus (srbsdv) in the white-backed planthopper (wbph) and in rice plants both altered the vector's feeding behavior. when viruliferous wbph (carrying srbsdv) were fed on uninfected plants, they spent more time in salivation and phloem sap ingestion than non-virul ... | 2016 | 27492995 |
| a phloem-limited fijivirus induces the formation of neoplastic phloem tissues that house virus multiplication in the host plant. | a number of phloem-limited viruses induce the development of tumours (enations) in the veins of host plants, but the relevance of tumour induction to the life cycle of those viruses is unclear. in this study, we performed molecular and structural analyses of tumours induced by rice black-streaked dwarf virus (rbsdv, genus fijivirus) infection in maize plants. the transcript level of the maize cdc2 gene, which regulates the cell cycle, was highly elevated in tumour tissues. two-dimensional electr ... | 2016 | 27432466 |
| hairpin rna targeting multiple viral genes confers strong resistance to rice black-streaked dwarf virus. | rice black-streaked dwarf virus (rbsdv) belongs to the genus fijivirus in the family of reoviridae and causes severe yield loss in rice-producing areas in asia. rna silencing, as a natural defence mechanism against plant viruses, has been successfully exploited for engineering virus resistance in plants, including rice. in this study, we generated transgenic rice lines harbouring a hairpin rna (hprna) construct targeting four rbsdv genes, s1, s2, s6 and s10, encoding the rna-dependent rna polyme ... | 2016 | 27187354 |
| identification and characterization of micrornas in the white-backed planthopper, sogatella furcifera. | micrornas (mirnas) are a novel class of small, non-coding endogenous rnas that play critical regulatory roles in many metabolic activities in eukaryotes. reports of the identification of mirnas in sogatella furcifera (white-backed planthopper), the insect that acts as the only confirmed vector of the southern rice black-streaked dwarf virus (srbsdv), are limited. in this study, a total of 382 mirnas were identified in s. furcifera, including 106 conserved and 276 novel mirnas, using high-through ... | 2016 | 27060479 |
| [development of transgenic maize with anti-rough dwarf virus artificial mirna vector and their disease resistance]. | maize is one of the most important food crops. rice black-streaked dwarf virus is a maize rough dwarf disease pathogen. the occurrence and transmission of maize rough dwarf disease brings great damage to maize production. the technology of using artificial mirna to build antiviral plant has been proven effective in a variety of plants. however, such trials in maize have not been reported. we designed primers based on the sequence of maize zea-mir159a precursor and sequence of function protein ge ... | 2015 | 26955715 |
| complete genomic sequence of maize rough dwarf virus, a fijivirus transmitted by the small brown planthopper. | the nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus (mrdv) were determined. this first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. | 2016 | 26847903 |
| reference gene selection and evaluation for gene expression studies using qrt-pcr in the white-backed planthopper, sogatella furcifera (hemiptera: delphacidae). | the white-backed planthopper, sogatella furcifera (hemiptera, delphacidae), is one of the most devastating rice pests. for a better control strategy, various genetic studies have been conducted using reverse-transcription quantitative real-time polymerase chain reaction (qrt-pcr). the appropriate application of qrt-pcr requires reliable endogenous controls; however, studies on this aspect of the white-backed planthopper are lacking. in the present study, nine commonly used reference genes, elong ... | 2016 | 26612891 |
| assessment of reference gene stability in rice stripe virus and rice black streaked dwarf virus infection rice by quantitative real-time pcr. | stably expressed reference gene(s) normalization is important for the understanding of gene expression patterns by quantitative real-time pcr (rt-qpcr), particularly for rice stripe virus (rsv) and rice black streaked dwarf virus (rbsdv) that caused seriously damage on rice plants in china and southeast asia. | 2015 | 26497487 |
| relation between the viral load accumulation of southern rice black-streaked dwarf virus and the different developmental stages of sogatella furcifera (hemiptera: delphacidae). | the white-backed planthopper, sogatella furcifera (horvath), is currently the only confirmed vector of southern rice black-streaked dwarf virus (srbsdv), which causes severe rice production losses in china. in this study, an absolute quantification qpcr method was used to detect viral gene mrna expression levels at different developmental stages of white-backed planthoppers fed srbsdv-infected rice plants. a comparison of viral copy numbers of the srbsdv s10 gene at the same developmental stage ... | 2015 | 26470211 |
| argonaute subfamily genes in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | argonaute (ago) proteins are essential catalytic components of the rna-induced silencing complex and play central roles in rna interference. using a combination of bioinformatics and rapid amplification of cdna ends (race) methods, putative ago subfamily members, ls-ago1 and ls-ago2, were cloned and characterized from the small brown planthopper, laodelphax striatellus. the open reading frame (orf) of ls-ago1 is 2,820 bp long, encoding a putative protein of 939 amino acid residues, and ls-ago2 c ... | 2016 | 26446351 |
| nac transcription factor family genes are differentially expressed in rice during infections with rice dwarf virus, rice black-streaked dwarf virus, rice grassy stunt virus, rice ragged stunt virus, and rice transitory yellowing virus. | expression levels of the nac gene family were studied in rice infected with rice dwarf virus (rdv), rice black-streaked dwarf virus (rbsdv), rice grassy stunt virus (rgsv), rice ragged stunt virus (rrsv), and rice transitory yellowing virus (rtyv). microarray analysis showed that 75 (68%) osnac genes were differentially regulated during infection with rdv, rbsdv, rgsv, and rrsv compared with the control. the number of osnac genes up-regulated was highest during rgsv infection, while the lowest n ... | 2015 | 26442000 |
| low temperature storage of southern rice black-streaked dwarf virus-infected rice plants cannot sustain virus transmission by the vector. | southern rice black-streaked dwarf virus (srbsdv) is a novel virus transmitted by white-backed planthopper sogatella furcifera (hováth) (hemiptera: delphacidae). due to low virus transmission efficiency by the planthopper, researchers are frequently confronted with shortage of viruliferous vectors or infected rice plants, especially in winter and the following spring. to find new ways to maintain virus-infected materials, viral rice plants were stored at -80°c for 45 or 140 d and evaluated as vi ... | 2016 | 26405060 |
| rice responses and resistance to planthopper-borne viruses at transcriptomic and proteomic levels. | rice (oryza sativa) is one of the most important cereal crops in the world, especially in asian areas. rice virus diseases are considered as the most serious threat to rice yields. most rice viruses are transmitted by hemipteran insects such as planthoppers and leafhoppers. in asia five rice viruses are transmitted mainly by three planthopper species in a persistent manner: rice stripe virus, rice black-streaked dwarf virus, rice ragged stunt virus, rice grassy stunt virus, and southern rice bla ... | 2016 | 26363817 |
| interactive effects of southern rice black-streaked dwarf virus infection of host plant and vector on performance of the vector, sogatella furcifera (homoptera: delphacidae). | performance of insect vectors can be influenced by the viruses they transmit, either directly by infection of the vectors or indirectly via infection of the host plants. southern rice black-streaked dwarf virus (srbsdv) is a propagative virus transmitted by the white-backed planthopper, sogatella furcifera (hovath). to elucidate the influence of srbsdv on the performance of white-backed planthopper, life parameters of viruliferous and nonviruliferous white-backed planthopper fed rice seedlings i ... | 2014 | 26309259 |
| identification and expression profiling analysis of calmodulin-binding transcription activator genes in maize (zea mays l.) under abiotic and biotic stresses. | the calmodulin-binding transcription activators (camta) play critical roles in plant growth and responses to environmental stimuli. however, how camtas function in responses to abiotic and biotic stresses in maize (zea mays l.) is largely unknown. in this study, we first identified all the camta homologous genes in the whole genome of maize. the results showed that nine zmcamta genes showed highly diversified gene structures and tissue-specific expression patterns. many zmcamta genes displayed h ... | 2015 | 26284092 |
| genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice. | rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ... | 2015 | 26198760 |
| whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper. | rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ... | 2015 | 26149652 |
| molecular genetic analysis and evolution of segment 7 in rice black-streaked dwarf virus in china. | rice black-streaked dwarf virus (rbsdv) causes maize rough dwarf disease or rice black-streaked dwarf disease and can lead to severe yield losses in maize and rice. to analyse rbsdv evolution, codon usage bias and genetic structure were investigated in 111 maize and rice rbsdv isolates from eight geographic locations in 2013 and 2014. the linear dsrna s7 is a+u rich, with overall codon usage biased toward codons ending with a (a3s, s7-1: 32.64%, s7-2: 29.95%) or u (u3s, s7-1: 44.18%, s7-2: 46.06 ... | 2015 | 26121638 |
| quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. | southern rice black streaked dwarf virus (srbsdv) and rice black streaked dwarf virus (rbsdv) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. in this study, we quantitatively compared the spatial distribution of srbsdv and rbsdv contents over time in their vector and nonvector insects using real time-pcr. genome equivalent copies (gec) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period ( ... | 2015 | 26116274 |
| studies of binding interactions between dufulin and southern rice black-streaked dwarf virus p9-1. | southern rice black-streaked dwarf virus (srbsdv) is an important rice virus that can cause considerable crop losses. during infection, srbsdv p9-1 accumulates viroplasms in srbsdv-infected plant and insect cells, which may facilitate the formation of srbsdv particles. our field trial results showed that dufulin inhibited infection activity in srbsdv-infected rice. however it is not clear how dufulin inhibits the infection activity. therefore we studied the interactions between dufulin and srbsd ... | 2015 | 25934228 |
| characterization of the importance of terminal residues for southern rice black-streaked dwarf virus p9-1 viroplasm formations. | southern rice black-streaked dwarf virus (srbsdv) p9-1 is involved in viroplasm formations in the srbsdv-infected plants and insects. during infection, srbsdv p9-1 is an important protein. however, the function characterization of p9-1 octamers in vitro and in yeast is still obscure. in this study, the secondary and 3d structure of srbsdv p9-1 was predicted, then srbsdv p9-1 was expressed and analyzed in vitro, a size exclusion chromatography assay showed that p9-1 had the ability to form octame ... | 2015 | 25870931 |
| quantitative analysis of southern rice black-streaked dwarf virus in sogatella furcifera and virus threshold for transmission. | we investigated southern rice black-streaked dwarf virus (srbsdv) accumulation in a vector insect, the whitebacked planthopper (sogatella furcifera), to elucidate the association of virus accumulation in the vector with virus transmission efficiency. real-time quantitative reverse transcription polymerase chain reaction analysis confirmed that this virus is transmitted in a persistent-propagative manner. srbsdv was successfully transmitted by s. furcifera males in which rna accumulation of the c ... | 2015 | 25870927 |
| presence of poly(a) tails at the 3'-termini of some mrnas of a double-stranded rna virus, southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv), a new member of the genus fijivirus, is a double-stranded rna virus known to lack poly(a) tails. we now showed that some of srbsdv mrnas were indeed polyadenylated at the 3' terminus in plant hosts, and investigated the nature of 3' poly(a) tails. the non-abundant presence of srbsdv mrnas bearing polyadenylate tails suggested that these viral rna were subjected to polyadenylation-stimulated degradation. the discovery of poly(a) tails in differen ... | 2015 | 25835534 |
| interaction research on the antiviral molecule dufulin targeting on southern rice black streaked dwarf virus p9-1 nonstructural protein. | southern rice black streaked dwarf virus (srbsdv) causes severe harm to rice production. unfortunately, studies on effective antiviral drugs against srbsdv and interaction mechanism of antiviral molecule targeting on srbsdv have not been reported. this study found dufulin (dfl), an ideal anti-srbsdv molecule, and investigated the interactions of dfl targeting on the nonstructural protein p9-1. the biological sequence information and bonding characterization of dfl to four kinds of p9-1 protein w ... | 2015 | 25807053 |
| p5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts. | the genome segment s5 of rice black-streaked dwarf virus (genus fijivirus, family reoviridae) is functionally bicistronic in infected plants. it has a conserved second orf (p5-2) partially overlapping the major orf in a different reading frame, but its function remains unknown. p5-2 was detected in infected plants, but not in purified viral particles by western blotting, indicating that it is a non-structural protein. in immunoelectron microscopy, polyclonal antibodies against p5-2 specifically ... | 2015 | 25749897 |
| first discovery of acetone extract from cottonseed oil sludge as a novel antiviral agent against plant viruses. | a novel acetone extract from cottonseed oil sludge was firstly discovered against plant viruses including tobacco mosaic virus (tmv), rice stripe virus (rsv) and southern rice black streaked dwarf virus (srbsdv). gossypol and β-sitosterol separated from the acetone extract were tested for their effects on anti-tmv and analysed by nuclear magnetic resonance (nmr) assay. in vivo and field trials in different geographic distributions and different host varieties declared that this extract mixture w ... | 2015 | 25705894 |
| development of proteomic technology of shotgun and label free combined with multiple reaction monitoring to simultaneously detect southern rice black-streaked dwarf virus and rice ragged stunt virus. | the co-infection of rice caused by southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) was widely found at many regions, such as yunnan province, china, and north and central vietnam. these rice viral diseases lead to seriously yield loss of rice. in this study, the proteomics technology of shotgun and label free combined with multiple reaction monitoring (mrm) was developed to detect rice sample of a single or/and co-infection. the shotgun assay indicated that s ... | 2014 | 25674599 |
| phylogenetic and recombination analysis of rice black-streaked dwarf virus segment 9 in china. | rice black-streaked dwarf virus (rbsdv) is an economically important virus that causes maize rough dwarf disease and rice black-streaked dwarf disease in east asia. to study rbsdv variation and recombination, we examined the segment 9 (s9) sequences of 49 rbsdv isolates from maize and rice in china. three s9 recombinants were detected in baoding, jinan, and jining, china. phylogenetic analysis showed that chinese rbsdv isolates could be classified into two groups based on their s9 sequences, reg ... | 2015 | 25633210 |
| genome-wide characterization of rice black streaked dwarf virus-responsive micrornas in rice leaves and roots by small rna and degradome sequencing. | micrornas (mirnas) are small, non-coding rnas which typically function by guiding cleavage of target mrnas. they play important roles in development, abiotic stress and responses to pathogens. four small rna libraries and four degradome libraries were constructed from the leaves and roots of healthy rice and plants infected with rice black streaked dwarf virus (rbsdv). analysis of the deep sequencing results showed that the expression patterns of 14 mirnas in leaves and 16 mirnas in roots change ... | 2015 | 25535197 |
| a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector. | rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ... | 2015 | 25455902 |
| characterization of homologous and heterologous interactions between viroplasm proteins p6 and p9-1 of the fijivirus southern rice black-streaked dwarf virus. | p6 of southern rice black-streaked dwarf virus (srbsdv) is a multifunctional protein that is involved in the formation of viroplasms by interacting with p5-1. here, we used yeast two-hybrid and bimolecular fluorescence complementation assays to show that there were homologous and heterologous interactions between srbsdv p6 and p9-1 in yeast and plant cells. mutational analysis showed that the n-terminal region (residues 1-93) of p6 was necessary for the interaction between p6 and p9-1. self-inte ... | 2015 | 25377635 |
| infection of rice plants by rice black streaked dwarf virus improves an egg parasitoid, anagrus nilaparvatae (hymenoptera: mymaridae), of rice planthoppers. | the effects of rice plants infected by rice black streaked dwarf virus (rbsdv) on the host preference, duration of immature stages, sex ratio, and adult longevity and parasitic capacity of an egg parasitoid, anagrus nilaparvatae pang et wang, of rice brown planthopper, nilaparvata lugens stål, were evaluated. tests of response to plant volatiles using an olfactometer showed that a. nilaparvatae preferred rice plants harboring rice brown planthopper eggs over plants free of rice brown planthopper ... | 2014 | 25199055 |
| virus-mediated chemical changes in rice plants impact the relationship between non-vector planthopper nilaparvata lugens stål and its egg parasitoid anagrus nilaparvatae pang et wang. | in order to clarify the impacts of southern rice black-streaked dwarf virus (srbsdv) infection on rice plants, rice planthoppers and natural enemies, differences in nutrients and volatile secondary metabolites between infected and healthy rice plants were examined. furthermore, the impacts of virus-mediated changes in plants on the population growth of non-vector brown planthopper (bph), nilaparvata lugens, and the selectivity and parasitic capability of planthopper egg parasitoid anagrus nilapa ... | 2014 | 25141278 |
| a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host. | rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ... | 2014 | 25075933 |
| micrornas responding to southern rice black-streaked dwarf virus infection and their target genes associated with symptom development in rice. | southern rice black-streaked dwarf virus (srbsdv) is a recently emerged rice virus that has spread across asia. this devastating virus causes rice plants to produce a variety of symptoms during different growth stages. micrornas (mirnas) comprise a large group of 21-24-nt rna molecules that are important regulators of plant development processes and stress responses. in this study, we used microarray profiling to investigate rice mirnas responding to srbsdv infection at 3, 9, 15, and 20 days pos ... | 2014 | 25038403 |