| cloning of the maize rough dwarf virus genome: molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains within a single genomic segment. | the segmented double-stranded rna genome of maize rough dwarf virus, a plant-infecting reovirus of the genus fijivirus, was cloned and partially characterized. nucleotide sequence analysis of full-length cdna clones corresponding to genomic segments s6, s7, and s8 revealed each segment to contain the conserved terminal oligonucleotide sequences (+) 5' aaguuuuuu------uguc 3' and adjacent, segment-specific, regions of inverted complementarity (inverted repeats), a structural motif previously repor ... | 1991 | 1989382 |
| in vitro transcription of the double-stranded rna genome of maize rough dwarf virus (reoviridae). | an rna-dependent rna polymerase associated with particles of maize rough dwarf virus, a fijivirus, was characterized using two in vitro assays differing in their energy regeneration systems. optimum reaction rates occurred at ph 8.0 to 8.5 at 20 degrees c. the presence of virus and mn2+ or mg2+ was essential for enzyme activity; mn2+ stimulated more incorporation events than mg2+, at optimum concentrations of 2 to 4 mm and 4 mm, respectively. incorporation was not affected by alpha-amanitin, act ... | 1990 | 2313268 |
| serological relationship between maize rough dwarf virus and rice black-streaked dwarf virus. | | 1973 | 4215213 |
| fractionation of the double-stranded rna of maize rough dwarf virus subviral particles. | | 1974 | 4363586 |
| enhancement of the immunogenicity of the maize rough dwarf virus outer shell with the cross-linking reagent dithiobis(succinimidyl)propionate. | injection into rabbits of native or glutaraldehyde (ga)-treated intact particles of the fijivirus maize rough dwarf virus (mrdv) failed to yield antibodies to the outer shell of the virus. after fixation of the particles with the cross-linking reagent dithiobis(succinimidyl)propionate (dsp), antisera were obtained reacting with the outer shell of mrdv (both dsp-fixed and unfixed) to a titre of 1/1024 in immunoelectron microscopic decoration tests, but giving no reaction with another fijivirus, o ... | 1981 | 6792213 |
| close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. | the complete nucleotide sequences of rice black-streaked dwarf virus (rbsdv) genome segments 8 (s8) and 7 (s7) were determined, and were found to have high sequence identities to the corresponding maize rough dwarf virus (mrdv) genome segments. rbsdv s8 and s7 consisted of 1927 and 2193 nucleotides, respectively. rbsdv s8 had a single long open reading frame (orf) encoding 591 amino acids. rbsdv s7 had two nonoverlapping orfs encoding 362 (orf1) and 309 (orf2) amino acids. the two orfs of rbsdv ... | 1993 | 8336114 |
| identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in china. | three virus isolates from maize with rough dwarf in different provinces in china were analyzed at the molecular level. when compared to an isolate from diseased rice plants in hubei province, all four isolates had identical genomic rna electrophoretic profiles, which were composed of ten double-stranded (ds) rnas. full-length cdnas of segment 10 (s10) from each of the four isolates were cloned by rt-pcr and the complete sequences were determined. analysis of the sequences revealed that each cons ... | 2001 | 11266211 |
| cloning and sequencing of the genome segment s9 of rice black-streaked dwarf virus. | genome segment s9's of rice black-streaked dwarf virus (rbsdv) of three chinese isolates were amplified by rt-pcr and sequenced, and were found to be consisted of 1 900 nt (rbsdv-zj s9 embl accession number aj297430),1 898 nt (rbsdv-heb s9 embl accession number aj297429) and 1 900 nt (rbsdv-hub s9 embl accession number aj291706), respectively. genome segment s9's of three chinese isolates shared 98.5%--98.8% sequence homology and all contained two open reading frames (orf), which encoded two pol ... | 2001 | 12040425 |
| identification of rice black streaked dwarf virus in different cereal crops with dwarfing symptoms in china. | this report describes isolation of virus particles from plants of rice, maize, wheat and sorghum with symptoms of dwarfing collected from two provinces of china, purification of double-stranded rna (dsrna) from the virus particles, and synthesis of full-length cdnas of genome segments 9 (s9) and 10 (s10) by reverse transcription-polymerase chain reaction (rt-pcr). sequence analysis showed that the s9 sequences of the chinese isolates and a japanese rice black-streaked disease virus (rbsdv) isola ... | 2001 | 12083334 |
| sequence analysis of genome segments s4 and s8 of mal de río cuarto virus (mrcv): evidence that the virus should be a separate fijivirus species. | this is the first sequence-based characterization of mal de río cuarto virus (mrcv), currently classified as a variant of maize rough dwarf virus (mrdv) and exclusively found in south america. we sequenced and analyzed genome segments s4 and s8. mrcv s4 coded for a putative 131.67 kda protein while mrcv s8 coded for a putative 68.26 kda protein containing an atp/gtp-binding motif. the 5' and 3' ends of mrcv segments, were 5'aaguuuuu3' and 5'cagcunnnguc3', respectively. prediction of secondary st ... | 2002 | 12209310 |
| phylogenetic analysis reveals that a dwarfing disease on different cereal crops in china is due to rice black streaked dwarf virus (rbsdv). | a viral disease with dwarfing symptoms is associated with severe damage of different cereal crops including rice, maize, wheat and sorghum grown in china. it is believed that the pathogenic agent of the disease on rice and sorghum is rice black streaked dwarf virus (rbsdv), however, the cause of maize dwarf disease in china is still inconclusive. in this report, dsrna was isolated from virus particles obtained from the diseased plants of rice, maize, wheat and sorghum from two chinese provinces. ... | 2002 | 12416683 |
| [cdna cloning and expression in escherichia coli of rice black-streaked dwarf virus segment 7]. | using primers designed from the terminal sequences of maize rough dwarf virus s6, a 2.2 kb cdna fragment was amplified by rt-pcr from maize plants showing maize rough dwarf disease. sequence analysis shows that the full length of this cdna is 2193bp. it contains two open reading frames that encoded two polypeptides with molecular weight of 41.0kd and 36.3kd, respectively. results of multi-sequences alignment suggest that, this cdna sequence has significant similarity to rice black-streaked dwarf ... | 2003 | 16276917 |
| molecular analysis of fiji disease virus segments 2, 4 and 7 completes the genome sequence. | the complete nucleotide sequences of fiji disease virus (fdv) genome segments s2, s4 and s7 were determined. this now completes the sequencing of all ten dsrna genome segments of the fijivirus type member, fdv, which comprises a total of 29339 nt. fdv s2, s4 and s7 comprised 3820, 3568 and 2194 nt, respectively. s2 and s4 each contained a single open reading frame (orf), which encoded putative proteins of 137 and 133 kda, respectively, while s7 contained two orfs, which encoded putative proteins ... | 2006 | 16525734 |
| sequencing of the bicistronic genome segments s7 and s9 of mal de río cuarto virus (fijivirus, reoviridae) completes the genome of this virus. | the nucleotide sequences of genomic segments s7 and s9 of mal de río cuarto virus (mrcv, fijivirus group ii) have been determined, thus completing the entire genome sequence of the virus. these segments showed a non-overlapping bicistronic structure, as in other members of the genus. mrcv s7 orf-1 had a length of 1086 bp and encoded a 41.5 kda putative polypeptide, whereas mrcv s7 orf-2 had a length of 930 bp and encoded a 36.8 kda putative polypeptide. proteins of 39 and 20.5 kda were predicted ... | 2007 | 17115303 |
| a black-streaked dwarf disease on rice in china is caused by a novel fijivirus. | an isolate of a plant reovirus causing severe stunting and dark leaf symptoms on rice from guangdong, china, was similar in virion morphology and serologically related to rice black-streaked dwarf virus (rbsdv). the electrophoretic profiles of genome segments of the two viruses in agarose or polyacrylamide gel were indistinguishable. the four genome segments of the new isolate corresponding to rbsdv s7-s10 were amplified by ligation rt-pcr and sequenced. the size and organization of each genome ... | 2008 | 18820828 |
| impact of 9 years of bt-maize cultivation on the distribution of maize viruses. | this study assesses the effect of bt-maize on the distribution of maize viruses. random surveys were conducted in spain between 2001 and 2006 to evaluate the occurrence of maize viruses in bt-maize cultivation areas and in areas where this crop had not been introduced. maize dwarf mosaic virus (mdmv) was the predominant virus in bt-areas, and maize rough dwarf virus (mrdv) was the most predominant one in non-bt-areas, with mrdv an emergent virus in both types of areas. a decline in the occurrenc ... | 2009 | 19067216 |
| synthesis and migration of maize rough dwarf virus in the host cell: an autoradiographic study. | | 2015 | 4427107 |
| electrophoretic separation of dsrna genome segments from fiji disease and maize rough dwarf viruses. | by employing two different buffer solutions for polyacrylamide gel electrophoresis, all genome segments of fiji disease and maize rough dwarf virus were separated. fiji disease virus contains ten genome segments with approximate genome molecular weights of 19.26 x 10(6) and 19.85 x 10(6), depending on the buffer employed for electrophoresis. maize rough dwarf virus possesses ten dsrna segments and according to the buffer employed for electrophoresis the approximate molecular weights of the genom ... | 1975 | 18621350 |
| identification and fine-mapping of a qtl, qmrdd1, that confers recessive resistance to maize rough dwarf disease. | maize rough dwarf disease (mrdd) is a devastating viral disease that results in considerable yield losses worldwide. three major strains of virus cause mrdd, including maize rough dwarf virus in europe, mal de río cuarto virus in south america, and rice black-streaked dwarf virus in east asia. these viral pathogens belong to the genus fijivirus in the family reoviridae. resistance against mrdd is a complex trait that involves a number of quantitative trait loci (qtl). the primary approach used t ... | 2013 | 24079304 |
| complete genomic sequence of maize rough dwarf virus, a fijivirus transmitted by the small brown planthopper. | the nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus (mrdv) were determined. this first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. | 2016 | 26847903 |
| double-stranded ribonucleic acid from carnation cryptic virus. | the nucleic acid of carnation cryptic virus has been identified as double-stranded rna on the basis of (i) susceptibility to rnase in 0.1 x ssc (ssc = 0.15 m nacl-0.015 m sodium citrate buffer, ph 7.0) and resistance to rnase in 2 x ssc and to dnase, (ii) reaction with an antiserum containing antibodies to dsrna, and (iii) electron microscopic appearance in nondenaturing media similar to that of the dsrna of maize rough dwarf virus. upon electrophoresis in 5% polyacrylamide gels carnation crypti ... | 1981 | 18635110 |
| complete nucleotide sequence of a french isolate of maize rough dwarf virus, a fijivirus member in the family reoviridae. | the complete nucleotide sequence of a french isolate of maize rough dwarf virus (mrdv) was determined by next-generation sequencing and compared with the single available complete sequence and with the partial sequences of two additional isolates available in online databases. | 2016 | 27445367 |
| identification of promoter motifs regulating zmeif4e expression level involved in maize rough dwarf disease resistance in maize (zea mays l.). | maize rough dwarf disease (mrdd, a viral disease) results in significant grain yield losses, while genetic basis of which is largely unknown. based on comparative genomics, eukaryotic translation initiation factor 4e (eif4e) was considered as a candidate gene for mrdd resistance, validation of which will help to understand the possible genetic mechanism of this disease. zmeif4e (orthologs of eif4e gene in maize) encodes a protein of 218 amino acids, harboring five exons and no variation in the c ... | 2013 | 23474695 |
| stimulation of jasmonic acid production in zea mays l. infected by the maize rough dwarf virus-río cuarto. reversion of symptoms by salicylic acid. | in the present paper we study the possible biological relevance of endogenous jasmonic acid (ja) and exogenous salicylic acid (sa) in a plant-microbial system maize-virus. the virus disease "mal de río cuarto" is caused by the maize rough dwarf virus-río cuarto. the characteristic symptoms are the appearance of galls or "enations" in leaves, shortening of the stem internodes, poor radical system and general stunting. changes in ja and protein pattern in maize control and infected plants of a vir ... | 2002 | 12619568 |
| [cdna cloning and sequence analysis of the seventh segment of maize rough dwarf virus genome]. | the double strand rna of maize rough dwarf virus (mrdv) was prepared from the maize samples showing symptoms which was from the luanchen county of heibei province of china. the primers were designed according to the known sequence of mrdv, the cdna sequence of the seventh segment of mrdv was obtained by rt-pcr, the s7 sequence was analyzed by computer after sequencing. the results showed: the full length of the s7 cdna is 1936 bp and equal to that of the s7 cdna from abroad, the two open reading ... | 2000 | 12548759 |
| x-ray microanalysis of leaf tumors from maize plants experimentally infected with maize rough dwarf virus: scanning and transmission electron microscopic study. | x-ray microanalysis, performed by both scanning and transmission electron microscopes equipped with energy-dispersive spectrometers (eds), was used to analyze the element distribution in phloem tumors induced by maize rough dwarf virus (mrdv) in zea mays l. the content of both si and ca was lower in tumors than in the nontumoral surrounding tissues. the si content increased in old tumors where this element was mainly concentrated in the trichomes growing at their surface. the peaks of other dete ... | 1980 | 18631653 |
| some properties of maize rough dwarf virus subviral particles. | | 1973 | 4147143 |
| partial purification, structure and infectivity of complete maize rough dwarf virus particles. | | 1973 | 4122423 |
| double-stranded ribonucleic acid from maize rough dwarf virus. | | 1972 | 4404094 |
| electron microscopy of maize rough dwarf virus assembly sites in maize. cytochemical and autoradiographic observations. | | 1972 | 4116130 |
| tubular structures associated with maize rough dwarf virus particles in crude extracts: electron microscopy study. | | 1971 | 4108671 |
| phases of maize rough dwarf virus multiplication in the vector laodelphax striatellus (fallén). | | 1970 | 5422718 |
| polyphenoloxidase activity in healthy maize plants and in those infected by the maize rough dwarf virus (mrdv). | | 1964 | 5856314 |