| identification of atypical rotaviruses in outbreaks of preweaning and postweaning diarrhea in québec swine herds. | intestinal contents of diarrheic pigs from 120 outbreaks of diarrhea were examined for the presence of atypical rotaviruses. pigs involved in these outbreaks were aged two days to five weeks and samples collected over a period of one year originated from different regions of the province of québec. samples were analyzed by polyacrylamide gel electrophoresis (page) for the presence of viral rna and genome profiles were compared to those of porcine rotavirus a/osu, b/ohio and c/cowden. based on el ... | 1991 | 1653639 |
| [heterologous system of immunoenzyme analysis for the diagnosis of human rotavirus infection and its comparison with rna electrophoresis]. | on the basis of porcine rotavirus a heterologous eia test system was worked out and tested for diagnosis of human rotavirus infection. a high sensitivity and specificity of the test system was demonstrated, its results were compared with those of electron microscopy, diffuse precipitation test, and rna electrophoresis. out of 201 specimens (fecal filtrates) collected from children ranging in ages from 14 days to 10 years, rotavirus antigen was detected in 68 (33.8%) rna electrophoresis demonstra ... | 1988 | 2835872 |
| [expression of recombinant vp6 gene of porcine rotavirus a with non-antibiotic lactobacillus vector in escherichia coli]. | the antigenic determinants of vp6 gene of porcine rotavirus a was amplified from infected ma 104 cell by the reverse transcription-polymerase chain reaction (rt-pcr), the product of which was a 1194bp cdna segment. using t-a cloning technique, the pcr product was cloned into pgem-t vector. cloning plasmid pgem-t-vp6 and the prokaryotic shuttle expression vector pw425t between e. coli and lactobacillus, were digested by saci and kpni double enzymes, respectively. the purified vp6 gene was subclon ... | 2005 | 16342756 |
| [expression and immunogenicity analysis of recombinant plasmid pw425et-vp7 of porcine rotavirus a in lactobacillus]. | we tested the antigenicity of porcine rotavirus vp7 gene expressed in lactobacillus. | 2008 | 19149168 |
| multiplex pcr and multiplex rt-pcr for inclusive detection of major swine dna and rna viruses in pigs with multiple infections. | multiplex pcr and multiplex rt-pcr were developed to identify nine viruses in pigs with multiple infections. these viruses are: porcine circovirus type 2 (pcv2), suid herpesvirus 1, porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus (prrsv), japanese encephalitis virus, porcine rotavirus a (porv-a), porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and getah virus. these methods were shown to be high specificity and sensitivity. in the ... | 2009 | 19467264 |
| annual changes in predominant genotypes of rotavirus a detected in the feces of pigs in various developmental stages raised on a conventional farm. | the goal of the present study was to improve understanding of the ecology of porcine rotavirus a (rva) infection in pigs raised on a conventional farrow-to-finish farm. we collected 145 fecal samples over a 3-year period from suckling pigs and their dams, and pigs at 30, 60, 90, 120, and 150 days of age. reverse transcriptase-polymerase chain reaction analysis revealed that 29 samples (20%) were positive for the viral vp7 gene. the detection rate of vp7 sequences was highest in 30-day-old pigs ( ... | 2012 | 23313539 |
| analysis of porcine rotavirus a enterotoxin gene for structure-function relationship. | rotavirus (rv) infected piglets are presumed to be the latent sources of heterologous rv infection to humans and other animals. in rvs, non-structural protein 4 (nsp4) is the major virulence factor with pleiotropic properties. in this study, we analyzed nsp4 gene from porcine rvs, isolated from diarrheic and non-diarrheic cases, at different levels of protein folding to explore any correlation with their diarrhea inducing capabilities and evolution of nsp4 in porcine population. the full length ... | 2017 | 28057906 |
| whole genome analysis provides evidence for porcine-to-simian interspecies transmission of rotavirus-a. | we report here whole genome analysis of a porcine rotavirus-a (rva) strain rva/pig-wt/kna/et8b/2015/g5p[13] detected in a diarrheic piglet, and nearly whole genome (except for vp4 gene) analysis of a simian rva strain rva/simian-wt/kna/08979/2015/g5p[x] detected in a non-diarrheic african green monkey (agm) on the island of st. kitts, caribbean region. strain et8b exhibited a g5-p[13]-i5-r1-c1-m1-a8-n1-t7-e1-h1 genotype constellation that was identical to those of brazilian porcine rva g5p[13] s ... | 2017 | 28039076 |
| phylogenetic inference of the porcine rotavirus a origin of the human g1 vp7 gene. | rotavirus a (rva) is an important cause of acute gastroenteritis in children worldwide. the most common vp7 genotype of human rva is g1, but g1 is rarely detected in porcine strains. to understand the evolutionary relationships between human and porcine g1 vp7 genes, we sequenced the vp7 genes of three japanese g1 porcine strains; the first two (prv2, s80b) were isolated in 1980 and the third (kyusyu-14) was isolated in 2001. then, we performed phylogenetic and in-silico structural analyses. all ... | 2016 | 26961591 |
| rapid detection and high occurrence of porcine rotavirus a, b, and c by rt-qpcr in diagnostic samples. | rotaviruses are important cause of diarrhea in animals, including humans. currently, rotavirus species a, b, c, e, and h (rva-rvc, rve, and rvh) have been identified in pigs. traditionally, rva has been considered the primary cause of diarrhea in pigs, and rvb and rvc had been described sporadically in pigs until recently. qualitative porcine rva, rvb, and rvc rt-pcr (rt-qpcr) assays were designed and 7508 porcine diarrheic samples, submitted to university of minnesota, were tested to estimate t ... | 2014 | 25194889 |
| development and validation of monoclonal antibody-based antigen capture elisa for detection of group a porcine rotavirus. | porcine rotavirus-a (porva) is one of the common causes of mild to severe dehydrating diarrhea, leading to losses in weaning and postweaning piglets. a rapid, highly specific, and sensitive antigen-capture enzyme-linked immunosorbent assay (ac-elisa) was developed for detection of porva, by using vp6 (a highly conserved and antigenic protein of group-a rotavirus)-directed rabbit polyclonal antibodies (capture antibody) and murine monoclonal antibodies (detector antibody). the detection limit of ... | 2017 | 28414586 |
| epidemiological analysis of porcine rotavirus a genotypes in germany. | group a porcine rotaviruses are a global threat to animal health in stock breeding. while certain genotypes have shown predominance in other countries, data from europe's second largest swine population is still scarce. therefore, porcine rotaviruses taken from different areas of germany were genotyped to create a basis for comparison with data from neighboring countries. in addition, the potential predominance and regionality based on regions (federal states) have been investigated by examining ... | 2018 | 29408039 |