| animal model of rotavirus infection in rabbits--protection obtained without shedding of viral antigen. | a small animal model was developed in order to investigate the pathogenesis and immunology of rotavirus infections and to study the interaction of different virus strains. seronegative rabbits of the breed french lop were used. two rabbit rotavirus strains, belonging to the same serotype, were used: 82/311 f and r-2, both isolated during diarrhoeal outbreaks in commercial rabbitries. the animals were inoculated orally. the viral shedding and the serological response was monitored by elisa. initi ... | 1989 | 2479363 |
| evidence of duplication and deletion in super short segment 11 of rabbit rotavirus alabama strain. | the complete nucleotide sequence of genome segment 11, of the alabama strain of rabbit rotavirus, was determined by sequencing dsrna and mrna using the dideoxy chain termination method. this strain has a "super short" rna pattern. its segment 11 is 1036 bp in length, 369 nucleotides longer than that reported for rotavirus strains with a "long" electropherotype. sequence analysis indicated that the shift in genome segment 11 of alabama strain to a "super short" rna pattern results from a simple d ... | 1989 | 2543133 |
| molecular characterization of three rabbit rotavirus strains. | we report biochemical (rna and protein patterns and gene-coding assignments) and serologic (serotype and subgroup) properties of three strains of rabbit rotaviruses--ala c11 and r2. the rna electropherotypes were a standard "short" pattern for r2, an unusual "short" pattern for ala, and an unusual "long" pattern for c11. serologic studies indicated that these viruses were all group a serotype 3 rotaviruses. in addition, the ala and c11 viruses were found to possess subgroup i specificity, wherea ... | 1988 | 2831858 |
| rabbit model of rotavirus infection. | a new small animal model was developed to study parameters of rotavirus infections, including the active immune response. seronegative new zealand white rabbits (neonatal to 4 months old) were inoculated orally with cultivatable rabbit rotavirus strains ala, c11, and r2 and with the heterologous simian strain sa11. the course of infection was evaluated by clinical findings, virus isolation (plaque assay and enzyme-linked immunosorbent assay), and serologic response. all four strains of virus wer ... | 1988 | 2833612 |
| isolation of two lapine rotaviruses: characterization of their subgroup, serotype and rna electropherotypes. | rotaviruses were detected by an elisa test in stool specimens from diarrheic rabbits in two commercial rabbitries and cultured in ma 104 cells. their identity was confirmed by electron microscopy and indirect immunofluorescence. they were found to belong to subgroup i by testing with monoclonal antibodies and to serotype 3 by neutralization with homologous and heterologous antisera. although both viruses were neutralized by antiserum to human serotype 3 the ala rabbit rotavirus was minimally neu ... | 1986 | 3013127 |
| comparative study of rotavirus strains of bovine and rabbit origin. | newborn calves were susceptible to infection with a rotavirus strain isolated from rabbits and, conversely, rabbits 4-5 weeks of age became ill and some died when inoculated with the rotavirus strain of bovine origin. however, the latter isolate appeared to be more virulent than the rabbit isolate in either calves or rabbits. moreover, in reciprocal cross protection tests, carried out in calves and in rabbits, the rabbit rotavirus antiserum afforded only weak protection to challenge infection wi ... | 1984 | 6099786 |
| analysis of host range restriction determinants in the rabbit model: comparison of homologous and heterologous rotavirus infections. | the main limitation of both the rabbit and mouse models of rotavirus infection is that human rotavirus (hrv) strains do not replicate efficiently in either animal. the identification of individual genes necessary for conferring replication competence in a heterologous host is important to an understanding of the host range restriction of rotavirus infections. we recently reported the identification of the p type of the spike protein vp4 of four lapine rotavirus strains as being p[14]. to determi ... | 1998 | 9499095 |
| determination of the duration of a primary immune response and the id50 of ala rabbit rotavirus in rabbits. | the rabbit model of rotavirus infection has been used to examine the immune response to rotavirus infection and to evaluate strategies for rotavirus vaccine development. to determine the 50% infectious does (id50) of tissue culture adapted ala virus, rabbit were orally inoculated with 10(1)-10(3) pfu of ala rotavirus. the id50 of ala virus was determined to be 1.7 x 10(2) pfu. the immune response induced in rabbits infected at low virus doses (10(2)-10(3) pfu) was of similar magnitude to the imm ... | 1997 | 9672593 |
| full genomic analysis of human rotavirus strain b4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission. | the belgian rotavirus strain b4106, isolated from a child with gastroenteritis, was previously found to have vp7 (g3), vp4 (p[14]), and nsp4 (a genotype) genes closely related to those of lapine rotaviruses, suggesting a possible lapine origin or natural reassortment of strain b4106. to investigate the origin of this unusual strain, the gene sequences encoding vp1, vp2, vp3, vp6, nsp1, nsp2, nsp3, and nsp5/6 were also determined. to allow comparison to a lapine strain, the 11 double-stranded rna ... | 2006 | 16571797 |
| complete genome analysis of a rabbit rotavirus causing gastroenteritis in a human infant. | group a rotaviruses (rva) are responsible for causing infantile diarrhea both in humans and animals. the molecular characteristics of lapine rva strains are only studied to a limited extent and so far g3p[14] and g3p[22] were found to be the most common g/p-genotypes. during the 2012-2013 rotavirus season in belgium, a g3p[14] rva strain was isolated from stool collected from a two-year-old boy. we investigated whether rva/human-wt/bel/be5028/2012/g3p[14] is completely of lapine origin or the re ... | 2015 | 25690801 |
| full genomic analysis of rabbit rotavirus g3p[14] strain n5 in china: identification of a novel vp6 genotype. | group a rotaviruses (rvas) are major pathogens associated with acute gastroenteritis in young children and in a wide variety of domestic animals. the full-length genome of a rabbit rva strain, rva/rabbit-tc/chn/n5/1992/g3p[14], showed a g3-p[14]-i17-r3-c3-m3-a9-n1-t1-e3-h2 genomic configuration. a novel vp6 genotype, i17, was confirmed by the rotavirus classification working group. phylogenetic analyses revealed that strain n5 possessed vp1-3, vp7, nsp1-2 and nsp4 genes closely related to those ... | 2012 | 22750552 |
| rapid detection of three rabbit pathogens by use of the luminex x-tag assay. | domestic rabbits especially new zealand white rabbits play an important role in biological research. the disease surveillance and quality control are essential to guarantee the results of animal experiments performed on rabbits. rabbit hemorrhagic disease virus, rabbit rotavirus and sendai virus are the important pathogens that needed to be eliminated. rapid and sensitive method focus on these three viruses should be established for routine monitoring. the luminex x-tag assay based on multiplex ... | 2018 | 29625588 |