| expression of the major inner capsid protein of the group b rotavirus adrv: primary characterization of genome segment 5. | a complete cdna copy of the fifth rna segment of the human group b rotavirus, adrv, has been cloned into plasmid ad512. gene segment 5 contains 1269 bases and encodes one long open reading frame of 391 amino acids beginning at base 31 and terminating at base 1203. the gene 5 polypeptide, expressed in vitro in a rabbit reticulocyte lysate, comigrates with the 44-kda major inner capsid protein present on edta treated adrv virions. the gene 5 protein is immunoprecipitable by hyperimmune serum to ad ... | 1991 | 1850929 |
| characterization of monoclonal antibodies to human group b rotavirus and their use in an antigen detection enzyme-linked immunosorbent assay. | three monoclonal antibodies (mabs)--b5c9, b5e4, and b10g10--to human group b rotavirus, an agent implicated in epidemic outbreaks of diarrhea in the people's republic of china, primarily in adults, were prepared. mab reactivity was decreased when virus preparations were treated with edta, suggesting reactivity with the outer-capsid protein(s). competition experiments suggested that these mabs recognize overlapping epitopes within a single antigenic site. a simple antigen detection enzyme-linked ... | 1989 | 2536755 |
| purification and characterization of adult diarrhea rotavirus: identification of viral structural proteins. | adult diarrhea rotavirus (adrv) is a newly identified strain of noncultivable human group b rotavirus that has been epidemic in the people's republic of china since 1982. we have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western (immuno-) blot analysis to examine the viral proteins present in the outer and inner capsids of adrv and compared these with the proteins of a group a rotavirus, sa11. edta treatment of double-shelled virions removed the outer capsid and resulted ... | 1989 | 2539512 |
| genetic and antigenic relatedness of human and animal strains of antigenically distinct rotaviruses. | we used nucleic-acid hybridization and enzymatic and immunofluorescence assay to examine the relatedness of human and animal strains of antigenically distinct rotaviruses (adrvs). adrvs isolated from rats and humans in baltimore, maryland, were shown to be closely related to bovine and porcine strains of group b rotavirus. a human group b rotavirus associated with epidemics of gastroenteritis in china was also found to share antigenic determinants and genome sequence homology with adrvs passaged ... | 1986 | 3023498 |
| cloning sequencing and expression of the gene encoding the vp2 protein of the human group b rotavirus, adrv. | adult diarrheal rotavirus, adrv, is a noncultivable human group b rotavirus. a complete cdna copy of adrv gene segment 2 has been cloned and sequenced. gene segment 2 contains 2844 bases and encodes one long open reading frame beginning at base 14 and terminating at base 2812. gene 2 encodes a protein containing 933 amino acids with a calculated molecular weight of 105.6 kda and a pl of 5.5. the gene 2 polypeptide contains significant homology with the vp2 protein which comprises the core of gro ... | 1994 | 8178482 |
| identification and baculovirus expression of the vp4 protein of the human group b rotavirus adrv. | a complete cdna copy of the fourth rna segment of the human group b rotavirus adult diarrheal rotavirus (adrv) has been cloned into lambda phage and excised into plasmid psk bluescript. gene segment 4 contains 2,303 bases and encodes one long open reading frame beginning at base 16 and terminating at base 2263. the encoded protein contains 749 amino acids, with a calculated molecular mass of 84.4 kda and a pi of 6.1. gene 4 cdna was inserted into a recombinant baculovirus via homologous recombin ... | 1993 | 8386274 |
| human group b rotavirus: adult diarrhea rotavirus. | | 1996 | 8758349 |
| amplification of various genes of human group b rotavirus from stool specimens by rt-pcr. | the detection of the human group b rotavirus (hugbr) cal strain from india has given us an opportunity to design suitable primers for the detection of hugbr since cal is the second hugbr detected until now, the chinese adult diarrhoea rotavirus (adrv) being the first reported human pathogen belonging to this group of viruses. the primers described here may thus be used for the detection of human group b rotaviruses by reverse transcription-pcr (rt-pcr) in a diagnostic laboratory. | 2000 | 10996114 |
| sequence analysis of genes encoding structural and nonstructural proteins of a human group b rotavirus detected in calcutta, india. | nucleotide sequences of rna segments encoding structural proteins(vp4, vp6, and vp7) and nonstructural proteins(nsp1 and nsp3) of a human group b rotavirus cal-1, which was detected in calcutta, india, were determined and their relatedness with cognate genes of other group b rotaviruses was analyzed. the cal-1 genes showed generally high sequence identities (more than 90%) to those of human group b rotavirus, adult diarrheal rotavirus (adrv) in china, while identities with bovine, murine, and ov ... | 2001 | 11468747 |
| human group b rotavirus infections cause severe diarrhea in children and adults in bangladesh. | human group b rotavirus was detected in 12 of 220 adult patients and 2 of 67 child patients with severe diarrhea in bangladesh. group b rotavirus may be virulent in both adults and children, and the virus may be an especially serious diarrheal agent in bangladesh. | 2003 | 12734276 |
| group b rotaviruses similar to strain cal-1, have been circulating in western india since 1993. | generally, group a rotaviruses are the most common cause of paediatric diarrhoea. however, group b rotavirus, adult diarrhoea rotavirus (adrv), was found to be involved in epidemics of severe gastroenteritis in several areas of china during 1982-1983 and had resulted in more than one million cases among adults as well as older children. human group b rotavirus has been rarely reported outside china, but has been detected first from five adults with diarrhoea in kolkata, india during 1997-1998 (s ... | 2004 | 15310177 |
| phylogenetic analysis of a human group b rotavirus wh-1 detected in china in 2002. | a human group b rotavirus strain wh-1 was detected in an adult sporadic case of diarrhea in wuhan, china in 2002. in this study, the gene sequences of wh-1 were determined in order to examine the phylogenetic relatedness to other human group b rotaviruses found previously in china (adrv, in 1982), india (cal-1, in 1998), and bangladesh (bang373, in 2000), as well as animal viruses, and to estimate the mutation rate of group b rotavirus. vp7 (major outer capsid protein) gene of wh-1 showed extrem ... | 2004 | 15484272 |
| molecular characterization of the rotavirus nsp4 enterotoxin homologue from group b rotavirus. | the rna segment (gene 10) from a human group b rotavirus which encodes the homologue of the rotavirus enterotoxin (nsp4) has been cloned and sequenced. the gene is of the same length (751 nucleotides) as its better-characterized group a rotavirus counterpart but shows minimal homology (approximately 10%) to it at the primary sequence level. despite this low level of sequence homology, secondary structure predictions for the group b protein (adrv-nsp4) showed a close similarity of structural feat ... | 2005 | 15845266 |
| comparison of nsp4 protein between group a and b human rotaviruses: detection of novel diarrhea-causing sequences in group b nsp4. | the human group b rotavirus is a causative agent of severe adult diarrhea. in this study, we analyzed the nsp4 structure of a group b rotavirus strain, cal-1, and determined whether enterotoxin activity was present in cal-1 nsp4. cal-1 nsp4 was comprised of 219 amino acids which was longer than group a and c rotavirus nsp4, and the primary structures of their sequences differed considerably. however, cal-1 nsp4 had an enterotoxin-like sequence (residues 106-127) that was only 27% identical to th ... | 2006 | 16132179 |
| rt-pcr based diagnosis revealed importance of human group b rotavirus infection in childhood diarrhoea. | human group b rotavirus was first identified as causative agent of a large outbreak of severe gastroenteritis affecting more than 1 million people, predominantly adults in china in 1982-1983. in spite of serological evidences for the presence of group b rotavirus in many countries of the world, the virus has been detected only from china, india and bangladesh, where most of the cases were from adults. | 2006 | 16765641 |
| detection of group b rotavirus in an adult with acute gastroenteritis in yangon, myanmar. | in yangon, myanmar, a human group b rotavirus was first detected in 2007 in a stool specimen from a sporadic case of acute gastroenteritis in an adult. the strain was designated as mmr-b1. the full-length sequences of the mmr-b1 genes encoding vp7, vp4 (vp5* and vp8*), vp6, and nsp4 were determined for genetic characterization. these four mmr-b1 genes showed considerable higher sequence identities (97.2-98.4%) to those of group b rotaviruses detected in india (cal-1 in 1998) and bangladesh (bang ... | 2009 | 19774684 |
| evidence for occurrence of human group b rotavirus in central india based on characterization of nsp2 gene. | human group b rotavirus (hugbr) was first described as the causative agent of severe gastroenteritis, affecting millions of people in china during 1982-1983. in spite of serological evidences for the presence of hugbr in many countries of the world, the virus has only been detected from china, bangladesh and some parts of india. the present study describes a hugbr (designated as mp-1 isolate) which was confirmed in an adult patient suffering from gastroenteritis in 2008 in madhya pradesh, centra ... | 2011 | 23637510 |
| genome characterization of turkey rotavirus g strains from the united states identifies potential recombination events with human rotavirus b strains. | rotavirus g (rvg) strains have been detected in a variety of avian species, but rvg genomes have been published from only a single pigeon and two chicken strains. two turkey rvg strains were identified and characterized, one in a hatchery with no reported health issues and the other in a hatchery with high embryo/poult mortality. the two turkey rvg strains shared only an 85.3 % nucleotide sequence identity in the vp7 gene while the other genes possessed high nucleotide identity among them (96.3- ... | 2017 | 29168675 |