| the reoviridae family. | the members of the reoviridae family are extremely varied in host ranges and have such diverse natural histories that it is compelling to conclude that their structural asset and replication strategy are uniquely successful in evolutionary terms. it follows that their study addresses fundamental aspects of virology, besides the ones which are customary with important pathogens affecting humans, animals and plants. we deal here with the present taxonomy of the family reoviridae and of its genera, ... | 1994 | 8001342 |
| coltiviruses isolated from mosquitoes collected in indonesia. | viruses with genomes consisting of 12 segments of double-stranded (ds) rna were isolated from mosquito pools collected in indonesia in 1980 and 1981. the genome segments of 11 virus isolates exhibited two distinct electrophoretic patterns in agarose gels, and the viruses probably represent distinct groups of coltiviruses. ten of the 11 virus isolates produced recognizably similar page profiles of two groups of 6 segments (6-6) with 3 distinct profile variants, and the remaining virus isolate dis ... | 1993 | 8102827 |
| arbovirus survey in china in recent ten years. | | 1996 | 8758350 |
| comparative sequence analysis of american, european and asian isolates of viruses in the genus coltivirus. | in this study, the basis for the classification of virus isolates grouped within the genus coltivirus, family reoviridae, is discussed. sequences of dsrna segments from american (segments 9-12), european (segment 12) and asian (segments 7-12) isolates were characterized and polythetic criteria were defined for their taxonomic classification. these criteria (including sequence analysis) permitted the different species to be distinguished and classified into two groups. in both groups, subgroups w ... | 1998 | 9780055 |
| molecular diagnosis of group b coltiviruses infections. | the group-b of genus coltivirus encompasses isolates from humans, ticks or mosquitoes collected in indonesia and china. subgroup-b1 includes the strain jkt/dsr-7075 and subgroup-b2 strains jkt/dsr-6423, jkt/dsr-6969, jkt/dsr-7043 and the banna virus. data are described for the pcr-based diagnosis of infection by group b coltiviruses. sets of primers were designed from the sequences of the 7th, 9th and 12th viral segments and rt pcr assays were developed. consensus primers permitted the detection ... | 1999 | 10488759 |
| complete sequence determination and genetic analysis of banna virus and kadipiro virus: proposal for assignment to a new genus (seadornavirus) within the family reoviridae. | arboviruses with genomes composed of 12 segments of double-stranded (ds) rna have previously been classified as members or probable members of the genus coltivirus within the family reoviridae: a number of these viruses have been isolated in north america and europe and are serologically and genetically related to colorado tick fever virus, the coltivirus type species. these isolates constitute subgroup a of the coltiviruses. the complete genome sequences are now presented of two asian arbovirus ... | 2000 | 10811934 |
| sequence determination and analysis of the full-length genome of colorado tick fever virus, the type species of genus coltivirus (family reoviridae). | the colorado tick fever virus (ctfv) is the type species of genus coltivirus, family reoviridae. its genome consisting of 12 segments of dsrna was completely sequenced. it was found to be 29,174 nucleotides long (the longest of all reoviridae genomes characterized to date). conserved sequences at the 5' end (sacuuuugy) and at the 3' end (wugcagus) of the 12 segments were identified. the analysis of the putative proteins deduced from the nucleotide sequences permitted to identify functional motif ... | 2000 | 10891382 |
| strategies for the sequence determination of viral dsrna genomes. | the genetic study of viruses having dsrna genomes is hampered by the technical difficulty of complete sequence determination of dsrna. optimised methods are described here for sequencing dsrnas, which meet three different situations: (1) genomes that can be obtained in fairly high amounts (>20 ng per separated segment); (2) genomes with limited amounts of rna that can be detected by electrophoretic gel separation and staining; (3) genomes that cannot be detected by electrophoretic gel separation ... | 2000 | 10996648 |
| genus coltivirus (family reoviridae): genomic and morphologic characterization of old world and new world viruses. | we report a genomic and morphologic study of the european eyach (eya) virus (genus coltivirus, family reoviridae) and a comparative analysis with the american colorado tick fever (ctf) virus (the type species of the genus). the previously established, but distant, antigenic relationship between these viruses was strengthened by genetic findings (presence of cognate genes, amino acid identity between 55 and 88%, similar conserved terminal motifs, suspected read-through phenomenon in segment 9 of ... | 2002 | 11958454 |
| [new subtype of coltivirus isolated from mosquitoes in the northeast part of china]. | the purpose of this study was to isolate new arbovirus from mosquitoes collected from the northeast part of china during the summer of 1996. | 1999 | 12569751 |
| [preparation and storage of coltivirus antigen and its application in detection of coltivirus antibodies in serum from patients]. | to prepare purified and concentrated coltivirus high titer antigen in order to further detect antibodies against coltivirus in serum sample of patients. | 2002 | 12665927 |
| recombinant vp7-based enzyme-linked immunosorbent assay for detection of immunoglobulin g antibodies to colorado tick fever virus. | vp6, vp7, vp9, vp10, vp11, and vp12 of colorado tick fever virus (ctf virus), a virus member of the genus coltivirus, family reoviridae, were expressed in bacteria with the pgex-4t-2 vector. a partial sequence of vp7 (designated pvp7) was chosen to elaborate an enzyme-linked immunosorbent assay (elisa) for detecting anti-ctf virus immunoglobulin g (igg) antibodies in humans. this was based on two observations: (i) among all expressed proteins, pvp7 showed the highest immunoreactivity to an anti- ... | 2003 | 12734255 |
| molecular characterization of dsrna segments 2 and 5 and electron microscopy of a novel reovirus from a hypovirulent isolate, w370, of the plant pathogen rosellinia necatrix. | a hypovirulent isolate, w370, of the white root rot fungus rosellinia necatrix has previously been shown to harbour 12 dsrna segments. in this study, complete nucleotide sequences of segments 2 and 5 of w370 dsrnas were determined. the nucleotide sequence of genome segment 2 was 3773 bases long with a single long open reading frame (orf) encoding 1226 amino acid residues with a predicted molecular mass of approximately 138.5 kda. the nucleotide sequence of segment 5 was 2089 bases long with a si ... | 2003 | 12917464 |
| a reovirus of the fungus cryphonectria parasitica that is infectious as particles and related to the coltivirus genus of animal pathogens. | rna viruses of filamentous fungi fall into two broad categories, those that contain double-stranded rna (dsrna) genomes in rigid particles and those that are more closely related to positive-sense, single-stranded rna viruses with dsrna replicative intermediates found within lipid vesicles. effective infectivity systems have been described for the latter, using rna transcripts, but not for the former. we report the characterization of a reovirus from cryphonectria parasitica, the filamentous fun ... | 2004 | 14694120 |
| conserved sequence motifs for nucleoside triphosphate binding unique to turreted reoviridae members and coltiviruses. | | 2004 | 15113934 |
| recombinant vp6-based enzyme-linked immunosorbent assay for detection of immunoglobulin g antibodies to eyach virus (genus coltivirus). | eyach virus (eyav) is a tick-borne virus belonging to genus coltivirus, family reoviridae. it was isolated in germany and france and has been suspected to be responsible for neurological diseases in humans. to date, there has been no relatively rapid and relatively specific serological assay for eyav. | 2004 | 15135744 |
| [investigation of arboviruses in lancang river down-stream area in yunnan province]. | to investigate the epidemic state of arboviruses in the downstream area of lancang river in yunnan province. | 2003 | 15340542 |
| [genotyping of the chinese isolates of coltivirus]. | to classify the chinese isolates of coltiviruses. | 2003 | 15340549 |
| tick-borne virus diseases of human interest in europe. | several human diseases in europe are caused by viruses transmitted by tick bite. these viruses belong to the genus flavivirus, and include tick-borne encephalitis virus, omsk haemorrhagic fever virus, louping ill virus, powassan virus, nairovirus (crimean-congo haemorrhagic fever virus) and coltivirus (eyach virus). all of these viruses cause more or less severe neurological diseases, and some are also responsible for haemorrhagic fever. the epidemiology, clinical picture and methods for diagnos ... | 2004 | 15606630 |
| [report of 5 cases with colti virus encephalitis in southeastern areas of guizhou province]. | | 2004 | 15631724 |
| the structure and function of the outer coat protein vp9 of banna virus. | banna virus (bav: genus seadornavirus, family reoviridae) has a double-shelled morphology similar to rotavirus and bluetongue virus. the structure of bav outer-capsid protein vp9 was determined by x-ray crystallography at 2.6 a resolution, revealing a trimeric molecule, held together by an n-terminal helical bundle, reminiscent of coiled-coil structures found in fusion-active proteins such as hiv gp41. the major domain of vp9 contains stacked beta sheets with marked structural similarities to th ... | 2005 | 15642258 |
| studies of coltivirus in china. | the purpose of this article is to review the developments of studies of coltivirus in china. | 2005 | 15820089 |
| coltiviruses and seadornaviruses in north america, europe, and asia. | coltiviruses are tickborne viruses of the genus coltivirus. the type species, colorado tick fever virus (from north america), has been isolated from patients with flulike syndromes, meningitis, encephalitis, and other severe complications. another coltivirus, eyach virus, has been isolated from ticks in france and germany and incriminated in febrile illnesses and neurologic syndromes. seadornaviruses are endemic in southeast asia, particularly indonesia and china. the prototype virus of the genu ... | 2005 | 16318717 |
| seroprevalence of tick-borne and mosquito-borne arboviruses in european brown hares in northern and western germany. | tríbec virus (kemerovo serogroup, genus orbivirus), eyach virus (genus coltivirus), and tahyna virus (california encephalitis serogroup, genus bunyavirus) are arthropod-borne viruses known to occur in germany. these viruses are also suspected to cause human disease. so far, no information is available on their geographical distribution in germany and their natural transmission cycles. a total of 166 sera from european brown hares (lepus europaeus) collected in seven districts of the federal stat ... | 2006 | 16530475 |
| [detection of banna virus-specific nucleic acid with taqman rt-pcr assay]. | to develop a rapid and more sensitive taqman rt-pcr assay specific for banna virus. | 2006 | 16642219 |
| isolation and molecular characterization of banna virus from mosquitoes, vietnam. | we isolated and characterized a banna virus from mosquitoes in vietnam; 5 strains were isolated from field-caught mosquitoes at various locations; banna virus was previously isolated from encephalitis patients in yunnan, china, in 1987. together, these findings suggest widespread distribution of this virus throughout southeast asia. | 2008 | 18680655 |
| [neglected arthropod-borne viral infections in the czech republic]. | central european encephalitis is the most common arthropod-borne virus disease in the czech republic, with the mean annual incidence of 6 cases per 100 000 population. however, seven less known arboviruses (flavivirus west nile, bunyavirus tahyna, bunyavirus batai, bunyavirus sedlec, bunyavirus lednice, orbivirus tribee, uukuvirus uukuniemi) also circulate in this country, of which west nile, tahyna, tribec and possibly batai have been reported to cause human disease. moreover, antibodies agains ... | 2008 | 18767374 |
| [isolation and identification of arboviruses from mosquito pools in some regions of liaoning province, china]. | to isolate and identify arboviruses from mosquito pools in some regions of liaoning province. | 2009 | 19565849 |
| [the first report of kadipiro virus isolation in china]. | 5 strains of virus isolated from culex tritaeniorhynchus, anopheles sinensis and armigeres subalbatus, which caused cytopathic effect in c6/36 cells, had been obtained in the survey of arboviruses in northwestern yunnan province. china. the virus particles displayed 70 nanometers diameter (n=7) with no envelope but spikes on the surfaces. rna-page of the genomes of the isolates showed 6-5-1 profile. a fragment of the 12th segment sequence was amplified by a pair of specific primers for kadipiro ... | 2009 | 19634758 |
| [isolation and identification of banna virus from mosquito for the first time in inner mongolia]. | to identify the virus isolated from a mosquito culex modestus collected from tongliao city of inner mongolia autonomous region. | 2009 | 20104749 |
| banna virus, china, 1987-2007. | banna viruses (bavs) have been isolated from pigs, cattle, ticks, mosquitoes, and human encephalitis patients. we isolated and analyzed 20 bavs newly isolated in china; this finding extends the distribution of bavs from tropical zone to north temperate climates and demonstrate regional variations in bav phylogeny and mosquito species possibly involved in bav transmission. | 2010 | 20202434 |
| medical microbiology | the family reoviridae is composed of eight genera: orthoreovirus, orbivirus,
coltivirus, rotavirus, aquareovirus, cypovirus, phytoreovirus, and
fijivirus. certain orthoreovirus, orbivirus,
coltivirus, and rotavirus species infect humans;
phytoreovirus and fijivirus species infect
plants and insects; the cypoviruses (the cytoplasmic polyhidrosis viruses) infect
insects, and aquareovirus ... | 1996 | 21413350 |
| characterization of the stop codon readthrough signal of colorado tick fever virus segment 9 rna. | termination codon readthrough is utilized as a mechanism of expression of a growing number of viral and cellular proteins, but in many cases the mrna signals that promote readthrough are poorly characterized. here, we investigated the readthrough signal of colorado tick fever virus (ctfv) segment 9 rna (seg-9). ctfv is the type-species of the genus coltivirus within the family reoviridae and is a tick-borne, double-stranded, segmented rna virus. seg-9 encodes a 36-kda protein vp9, and by readthr ... | 2011 | 22190746 |
| [isolation of coltivirus from mosquitoes in beijing and it's biological characteristics]. | two strains (beijing 95-70 and 95-75) of coltivirus were isolated from mosquitoes collected in beijing during summer-autumn in 1994. the viruses caused cytopathogenic effect on c6/36 cells but not on bhk-21 and vero cells, and were not lethal for new born mice and three week old mice. the isolates were resistant to 5'-idu and ether, but sensitive to acid ph 3.0. there were 12 segments rna shown by polyacrylamide gel electrophoresis (page) and page profile of the two isolates were similar (6-6). ... | 1997 | 15617249 |
| complete nucleotide sequences of genome segments 1 and 3 of rosellinia anti-rot virus in the family reoviridae. | the nucleotide sequences of genome segments 1 and 3 of rosellinia anti-rot virus (rarv) from a hypovirulent isolate, w370, of the plant pathogen rosellinia necatrix were determined. the complete nucleotide sequence of the genome segment 1 encoded a putative rna-dependent rna polymerase (rdrp). the deduced amino acid sequence of rdrp of rarv showed 29% identity with rdrps of colorado tick fever virus (ctfv) and european eyach virus (eyav) in the genus coltivirus, and identities of 23-21% with mem ... | 2004 | 15045563 |
| [eyach virus: relative of the colorado tick fever virus rediscovered in baden-württemberg]. | | 2003 | 14518442 |
| serologic and molecular diagnosis of colorado tick fever viral infections. | molecular and serologic methods usable for the biological diagnosis of coltivirus infection are reported. we designed a multiplex reverse transcription-polymerase chain reaction system that allowed the simultaneous and specific amplification of three genomic segments from as little as 0.01 plaque-forming units. another system in the s2 viral segment permitted the differential diagnosis of american and european viral isolates. we also discuss some improvements of previous elisas, and the results ... | 1998 | 9840594 |
| [erve and eyach: two viruses isolated in france, neuropathogenic for man and widely distributed in western europe]. | two arboviruses have been isolated from mayenne, western france, in 1981-1982, during systematic field surveys: eyach virus, a cultivirus from ixodes ricinus and i. ventalloi ticks, and erve virus, a nairovirus from the tissues of white-toothed shrews, crocidura russula. for many years, these two viruses were considered as "orphan viruses" without pathogenic power for man, since it became evident that they circulate actively in europe among populations of wild small and large mammals. moreover, ... | 1998 | 9673063 |
| medically important arboviruses of the united states and canada. | of more than 500 arboviruses recognized worldwide, 5 were first isolated in canada and 58 were first isolated in the united states. six of these viruses are human pathogens: western equine encephalitis (wee) and eastern equine encephalitis (eee) viruses (family togaviridae, genus alphavirus), st. louis encephalitis (sle) and powassan (pow) viruses (flaviviridae, flavivirus), lacrosse (lac) virus (bunyaviridae, bunyavirus), and colorado tick fever (ctf) virus (reoviridae, coltivirus). their scien ... | 1994 | 8118792 |
| recombinant vp9-based enzyme-linked immunosorbent assay for detection of immunoglobulin g antibodies to banna virus (genus seadornavirus). | banna virus (bav, genus seadornavirus, family reoviridae) is an arbovirus suspected to be responsible for encephalitis in humans. two genotypes of this virus are distinguishable: a (chinese isolate, bav-ch) and b (indonesian isolate, bav-in6969) which exhibit only 41% amino-acid identity in the sequence of their vp9. the vp7 to vp12 of bav-ch and vp9 of bav-in6969 were expressed in bacteria using pgex-4t-2 vector. vp9 was chosen to establish an elisa for bav, based mainly on two observations: (i ... | 2004 | 14715307 |
| baculovirus expression of the 11 mycoreovirus-1 genome segments and identification of the guanylyltransferase-encoding segment. | the type member mycoreovirus 1 (myrv-1) of a newly described genus, mycoreovirus, isolated from a hypovirulent strain 9b21 of the chestnut blight fungus, has a genome composed of 11 dsrna segments (s1-s11). all of the segments have single orfs on their capped, positive-sense strands. infection of insect cells by baculovirus recombinants carrying full-length cdnas of s1-s11 resulted in overexpression of protein products of the expected sizes, based on their deduced amino acid sequences. this expr ... | 2007 | 17170467 |
| epidemiology of colorado tick fever in montana, utah, and wyoming, 1995-2003. | colorado tick fever (ctf) is a biphasic, febrile illness caused by a coltivirus and transmitted by the rocky mountain wood tick, dermacentor andersoni, in the western united states and canada. symptoms generally include acute onset of fever, headache, chills, and myalgias; illness often lasts for 3 weeks or more. laboratory-confirmed cases of ctf were identified from public health department records in montana, utah, and wyoming, and from the centers for disease control and prevention diagnostic ... | 2010 | 19725767 |
| complete characterisation of the american grass carp reovirus genome (genus aquareovirus: family reoviridae) reveals an evolutionary link between aquareoviruses and coltiviruses. | an aquareovirus was isolated from several fish species in the usa (including healthy golden shiners) that is not closely related to members of species aquareovirus a, b and c. the virus, which is atypical (does not cause syncytia in cell cultures at neutral ph), was implicated in a winter die-off of grass carp fingerlings and has therefore been called 'american grass carp reovirus' (agcrv). complete nucleotide sequence analysis of the agcrv genome and comparisons to the other aquareoviruses show ... | 2008 | 18191982 |
| structural organization of an encephalitic human isolate of banna virus (genus seadornavirus, family reoviridae). | banna virus (bav) is the type species of the genus seadornavirus within the family reoviridae. the chinese bav isolate (bav-ch), which causes encephalitis in humans, was shown to have a structural organization and particle morphology reminiscent of that of rotaviruses, with fibre proteins projecting from the surface of the particle. intact bav-ch virus particles contain seven structural proteins, two of which (vp4 and vp9) form the outer coat. the inner (core) particles contain five additional p ... | 2005 | 15784909 |
| identification and functional analysis of vp3, the guanylyltransferase of banna virus (genus seadornavirus, family reoviridae). | banna virus (bav) particles contain seven structural proteins: vp4 and vp9 form an outer-capsid layer, whilst the virus core contains three major proteins (vp2, vp8 and vp10) and two minor proteins (vp1 and vp3). sequence analysis showed that vp3 contains motifs [kx(i/v/l)s] and (hx(n)h) that have previously been identified in the guanylyltransferases of other reoviruses. incubation of purified bav-ch core particles with [alpha-32p]gtp resulted in exclusive covalent labelling of vp3, demonstrati ... | 2005 | 15784908 |