| rna segment 5 of broadhaven virus, a tick-borne orbivirus, shows sequence homology with segment 5 of bluetongue virus. | the sequence of broadhaven (brd) virus segment 5, the major genetic determinant of serotype, is 1658 nucleotides in length and contains a single open reading frame (orf) having the coding capacity for a protein of mr 52.5k. comparison of the orf of segment 5 of brd virus with published sequences of bluetongue virus (btv) revealed 30% nucleotide homology and 31% amino acid homology with the protein encoded by segment 5 of btv serotype 10. significant homology was not shown with segment 2 of btv, ... | 1990 | 2171220 |
| isolation and characterization of temperature sensitive mutants of broadhaven virus, a kemerovo group orbivirus (family, reoviridae). | eighteen stable temperature sensitive (ts) mutants of broadhaven virus were isolated without the aid of mutagens. spontaneous mutants were detected using 41 degrees c as the nonpermissive temperature and 36 degrees c as the permissive temperature. high frequency pairwise recombination defined five recombination groups. four mutants belonged to group i, three to group ii, six to group iii, two to group iv, and two to group v. ts 7 was a possible double mutant representing lesions corresponding to ... | 1986 | 3014779 |
| comparison of the non-structural protein, ns1, of tick-borne and insect-borne orbiviruses. | the nucleotide sequence of rna segment 6 of broadhaven virus (brdv), a tick-borne orbivirus, was determined principally from two overlapping cdna clones and rna end sequence analysis. the genome segment is 1714 base pairs in length and has a coding capacity for a protein of 537 amino acids, having a net charge of +4.0 at neutral ph. comparison of the predicted amino acid sequence of brdv rna segment 6 with the ns1 sequence of insect-borne orbiviruses, bluetongue virus (btv), african horse sickne ... | 1995 | 7653106 |
| subcore- and core-like particles of broadhaven virus (brdv), a tick-borne orbivirus, synthesized from baculovirus expressed vp2 and vp7, the major core proteins of brdv. | the genes encoding the two major core proteins (vp2 and vp7) of broadhaven (brd) virus, a tick-borne orbivirus, were inserted into the genome of autographa californica nuclear polyhedrosis virus (acnpv) under the control of copies of the acnpv polyhedrin promoter to produce two recombinant baculoviruses. infection of spodoptera frugiperda (sf) cells with a recombinant acnpv that synthesized brdv vp2 produced large numbers of brdv subcore-like particles. co-infection of cells with the two recombi ... | 1994 | 8079519 |
| structure of broadhaven virus by cryoelectron microscopy: correlation of structural and antigenic properties of broadhaven virus and bluetongue virus outer capsid proteins. | the three-dimensional structure of broadhaven virus (brdv) has been determined to 23 a resolution by cryoelectron microscopy and image processing. as predicted from sequence homology, the brdv structure resembles that of bluetongue virus (btv) with the notable exception of one of the outer shell proteins. the cores of brdv and btv are identical at medium resolution; they have a diameter of 710 a and the vp7 trimers are arranged on a t = 13 icosahedral lattice. the outer shell proteins, vp5 of br ... | 1997 | 9281498 |
| ticks associated with macquarie island penguins carry arboviruses from four genera. | macquarie island, a small subantarctic island, is home to rockhopper, royal and king penguins, which are often infested with the globally distributed seabird tick, ixodes uriae. a flavivirus, an orbivirus, a phlebovirus, and a nairovirus were isolated from these ticks and partial sequences obtained. the flavivirus was nearly identical to gadgets gully virus, isolated some 30 year previously, illustrating the remarkable genetic stability of this virus. the nearest relative to the orbivirus (for w ... | 2009 | 19194498 |
| complete sequence of great island virus and comparison with the t2 and outer-capsid proteins of kemerovo, lipovnik and tribec viruses (genus orbivirus, family reoviridae). | the complete nucleotide sequence of great island virus (giv) genome was determined, along with genome segments (seg) 1, 2 and 6 of kemerovo (kemv), lipovnik (lipv) and tribec (trbv) viruses. all four viruses, together with broadhaven virus, are currently classified within the species great island virus and have been isolated from ticks, birds or humans. sequence comparisons showed that seg-4 of giv encoded the outer-capsid protein responsible for cell attachment, although it was approximately ha ... | 2010 | 20739272 |