| features of the mammalian orthoreovirus 3 dearing l1 single-stranded rna that direct packaging and serotype restriction. | a series of recombinant mammalian orthoreoviruses (mammalian orthoreovirus 3 dearing, mrv-3de) were generated that express an mrv-3de lambda3-cat fusion protein. individual viruses contain l1cat double-stranded (ds) rnas that range in length from a minimum of 1020 bp to 4616 bp. the engineered dsrnas were generated from in vitro-transcribed single-stranded (ss) rnas and incorporated into infectious virus particles by using reverse genetics. in addition to defining the sequences required for thes ... | 2007 | 18024910 |