| a photo-cidnp study of the interaction of oligonucleotides with gene-5 protein of bacteriophage m13. | it is shown that photo-cidnp effects (cidnp, chemically induced dynamic nuclear polarization) can be generated in the 360-mhz proton nmr spectrum of gene-5 protein from bacteriophage m13. this technique is used to determine the number of tyrosyl residues at the surface of the protein and to assign the resonances from the 3,5-ring protons of these residues. the dna-binding site of the protein is investigated by formation of complexes with oligonucleotides. complex formation leads to shifting and/ ... | 1978 | 364473 |
| nucleotide sequence of the recognition site of the b-specific restriction modification system in e. coli. | two sb mutations in the genome of bacteriophage fd were located by sequence analysis in the fd sequence at positions 971 and 6341. base changes at or close to these positions in phage m13 and in phage fl am 124 also correlate with a loss of sensitivity to b restriction. from the sequence homology between the sequences at the two sb sites the recognition signal for the e. coli b restriction/modification enzzyme is predicted to be: 5' tga---8n---tgct 3' 3' act---8n---acga 5'. | 1979 | 374993 |
| expression of bacteriophage m13 dna in vivo. i. synthesis of phage-specific rna and protein in minicells. | it is demonstrated that after infection of the appropriate minicell-producing strain of escherichia coli with the filamentous bacteriophage m13, its replicative form dna is segregated into minicells. consequently these minicells have acquired the capability to direct the synthesis of phage-specific rna and protein. comparision of the electrophoretic mobilities of phage-specific rna species made in vitro with those made in m13 replicative form dna harbouring minicells, have indicated that almost ... | 1978 | 363158 |
| replication of bacteriophage m13. xiv. differential inhibition of the replication of m13 and m13 miniphage in a mutant of escherichia coli defective in the 5' leads to 3' exonuclease associated with dna polymerase i. | previous studies have shown that m13 single-strand synthesis is inhibited at nonpermissive temperature in escherichia coli polaexl, a temperature-sensitive mutant defective in the 5' leads to 3' exonuclease activity of polymerase i (t.-c. chen and d. s. ray, j. mol. biol. 106:589-604, 1976). under these conditions the formation of covalently closed replicative form (rf) molecules is greatly reduced, and miniature forms of rf accumulate. we show here that the accumulation of mini-rfs is the conse ... | 1978 | 366176 |
| insertion of the tn3 transposon into the genome of the single-stranded dna phage m13. | the transposable genetic element tn3, which carries an ampicillin (ap) resistance determinant, has been translocated from a cole1-apr plasmid, rsf2124, to the genome of the filamentous single-stranded dna phage m13. the site orientation of the inserted element has been determined for one such phage, m13::tn3-15. the insertion is within the intergenic space separating genes 2 and 4 and containing both the viral strand and complementary strand origins. the lengths of both the filamentous phage and ... | 1978 | 363518 |
| replication of bacteriophage m13. xii. in vivo cross-linking of a phage-specific dna binding protein to the single-stranded dna of bacteriophage m13 by ultraviolet irradiation. | | 1977 | 336896 |
| soluble precursor of an integral membrane protein: synthesis of procoat protein in escherichia coli infected with bacteriophage m13. | prior to virus assembly, the major coat protein of coliphage m13 is an integral protein of the host cytoplasmic membrane. coat protein synthesized in vitro is initially made with an nh2-terminal "leader peptide" of 23 amino acids and is termed "procoat." we now report that procoat is a biosynthetic precursor of coat protein in vivo. conversion of procoat to coat occurs within 30 sec in cells infected with wild-type virus. this proteolytic processing is delayed in cells infected by m13 mutants (i ... | 1979 | 375229 |
| translational and post-translational cleavage of m13 procoat protein: extracts of both the cytoplasmic and outer membranes of escherichia coli contain leader peptidase activity. | the coat protein of coliphage m13 is an integral protein of the host cytoplasmic membrane at all stages of the infectious cycle. both in in vivo and dna-directed in vitro synthesis, it is initially made with an nh2-terminal "leader peptide" of 23 amino acids and is termed procoat. we now report that leader peptidase, and activity which removes the leader peptide and converts procoat to coat, is found in both the inner (cytoplasmic) and outer membrane of escherichia coli. however, only cytoplasmi ... | 1979 | 370824 |
| comparison of specific binding sites for escherichia coli rna polymerase with naturally occurring hairpin regions in single-stranded dna of coliphage m13. | | 1978 | 353054 |
| function of phospholipids in escherichia coli. characterization of a mutant deficient in cardiolipin synthesis. | screening of a collection of temperature-sensitive mutants of escherichia coli for defects in phospholipid metabolism led to the isolation of a mutant deficient in cardiolipin synthesis. the defective gene, named cls, is closely linked to the trp marker and maps at about minute 27 on the e. coli chromosome. after transfer of cls to a defined genetic background by transduction, the mutant has the following properties as compared to an isogenic wild type. exponentially growing cells show a reducti ... | 1978 | 353047 |
| filamentous coliphage m13 as a cloning vehicle: insertion of a hindii fragment of the lac regulatory region in m13 replicative form in vitro. | a hindii restriction fragment comprising the escherichia coli lac regulatory region and the genetic information for the alpha peptide of beta-galactosidase (beta-d-galactosidegalactohydrolase, ec. 3.2.1.23) has been inserted into 1 of the 10 bsu i cleavage sites of m13 by blunt end ligation. a stable hybrid phage was isolated and identified by its ability to complement the lac alpha function. further characterization of the hybrid phage includes retransformation studies, agarose gel electrophore ... | 1977 | 333444 |
| base-unpaired regions in supercoiled replicative form dna of coliphage m13. | superhelical covalently closed circular replicative form dna (rf i) of coliphage m13 appears as a relaxed molecule that has a base-unpaired region in the form of a bubble (100 to 200 base pairs long) seen in electron micrographs when spread in the presence of formaldehyde and formamide or after pretreatment with glyoxal. s1 endonuclease, specific for single-stranded dna, converts superhelical m13 rf i dna, but not nonsuperhelical m13 rf i to a significant extent, into unit-length linear molecule ... | 1977 | 328505 |
| inhibition of bacteriophage m13 and phix174 duplex dna replication and single-strand synthesis in temperature-sensitive dnaz mutants of escherichia coli. | a functional dnaz product, known to be essential for host dna polymerization and for the synthesis of m13 and phix174 parental replicative-form (rf) dna, is required also for rf replication and single-strand synthesis by both of these phages. all three stages of m13 and phix174dna replication (parental rf formation, rf replication, and single-strand synthesis) are inhibited in dnazts mutants at elevated temperatures. in addition, the thermolabile step in m13 parental rf formation appears to occu ... | 1977 | 323515 |
| stability of the carrier state in bacteriophage m13-infected cells. | bacteriophage m13-infected carrier cells were shown to be unstable to prolonged growth under all conditions. carrier hfr cells were transferred in dilute culture (10(3) to 10(4)/ml), where reinfection was impossible and the physiology of the cell was minimally altered. after an initial period of about 10 generations, during which all cells in the culture remained infected, there was exponential decay in the proportion of infected cells in the culture. uninfected cells that appeared were m13 sens ... | 1977 | 321804 |
| nucleotide sequence of gene vii and of a hypothetical gene (ix) in bacteriophage m13. | a dna fragment containing gene vii of bacteriophage m13 has been transcribed and the nucleotide sequence of this 169-nucleotides long transcript was determined by rna sequencing methods. additionally, the nucleotide sequence of this gene and parts of its neighbouring genes v and viii has been determined by the dimethylsulphate-hydrazine technique. the reading frame of gene vii has been established by determining the nucleotide changes occurring in the transcripts of two amber mutants of this gen ... | 1978 | 370777 |
| cloning of a functional replication origin of phage g4 into the genome of phage m13. | | 1979 | 231682 |
| effect of escherichia coli dna binding protein on the transcription of single-stranded phage m13 dna by escherichia coli rna polymerase. | | 1977 | 334166 |
| the role of dna polymerase i-associated 5'-exonuclease in replication of coliphage m13 replicative-form dna. | | 1977 | 334178 |
| structure of nascent replicative form dna of coliphage m13. | nascent replicative form type ii (rfii) dna of coliphage m13 synthesized in an escherichia coli mutant deficient in the 5' leads to 3' exonuclease associated uith dna polymerase i contains ribonucleotides that are retained in the covalently closed rfi dna sealed in vitro by the joint action of t5 phage dna polymerase and t4 phage dna ligase. these rfi molecules are labile to alkali and rnase h, unlike the rfi produced either in vivo or from rfii with e. coli dna polymerase i and e. coli dna liga ... | 1978 | 272630 |
| photoreactive labeling of m13 coat protein in model membranes by use of a glycolipid probe. | coliphage m13 coat protein in synthetic bilayer membranes was labeled by use of 12-(4-azide-2-nitrophenoxy)stearoyl[1-14c]glucosamine, a photoreactive glycolipid probe that spontaneously inserts into membranes. in this model system, the probe preferentially labeled the proteins over the lipids. experiments designed to test the probe's restriction to integral membrane proteins revealed that extrinsic proteins as well as external peptide fragments of integral membrane proteins were not accessible ... | 1979 | 293655 |
| asymmetric orientation of a phage coat protein in cytoplasmic membrane of escherichia coli. | the coat protein of a filamentous phage (m13) enters the cytoplasmic membrane from two directions: from the outside upon infection and from the cell interior late in the viral life cycle prior to phage assembly and extrusion. binding of 125i-labeled anti-coat protein antibody to spheroplasts or to inverted vesicles was used to assay the orientation of coat protein in the membrane. both parental and newly synthesized coat protein were found to be exposed on the outer surface of the cytoplasmic me ... | 1975 | 54916 |
| membrane-associated assembly of m13 phage in extracts of virus-infected escherichia coli. | assembly of coliphage m13 is known to occur as the viral dna crosses the cytoplasmic membrane, shedding its virus-coded dna unwinding protein and acquiring from the membrane approximately 2400 copies of the major coat protein. conditions are described in which extracts of m13-infected e. coli and membranes prepared from such extracts will support virus assembly at a rate equivalent to that of intact cells. extracts prepared from cells infected with temperature-sensitive m13 mutants in genes 1, 3 ... | 1977 | 15248 |
| construction of an m13 histidine-transducing phage: a single-stranded cloning vehicle with one ecori site. | in order to create a ready source of single-stranded dna for dna sequence determination by the dideoxy chain-termination method, the promoter-proximal part of the histidine operon, the hisogd region of salmonella typhimurium, was cloned onto the single-stranded phage m13. both orientations of the his dna were cloned to supply dna template for sequencing of each strand. insertion was achieved at an haeiii site in the intergenic region (ir) of m13, and a single ecori site was purposely regenerated ... | 1979 | 376403 |
| molecular basis of the am8h1 lesion in bacteriophage m13. | | 1979 | 462808 |
| nucleotide sequence of the genes iii, vi and i of bacteriophage m13. | a dna region of 2750 base pairs encompassing the genes iii, vi and i of bacteriophage m13 has been sequenced by the maxam-gilbert procedure. by establishing the nucleotide changes introduced by several amber mutations, the coding region and the regulatory signals of each gene have been deduced. the genes appear to span 1275 base pairs (gene iii; mol.wt. 44,748) 339 base pairs (gene vi; mol.wt. 12,264) and 1047 base pairs (gene i; mol.wt. 39,500). their separating non-codogenic regions are extrem ... | 1979 | 379830 |
| isolation and characterization of naturally occurring hairpin structures in single-stranded dna of coliphage m13. | | 1977 | 603641 |
| identification of two new capsid proteins in bacteriophage m13. | | 1979 | 387445 |
| a fast and simple method for sequencing dna cloned in the single-stranded bacteriophage m13. | | 1979 | 448736 |
| synthesis of phage m13 coat protein and its assembly into membranes in vitro. | the coat protein (gene 8 product) of coliphage m1o is an integral protein of the host cell membrane at all stages of virus infection. this protein, when made in a cell-free reaction, has been shown by others to have an additional nh2-terminal peptide region and is referred to as "procoat." it is initially not membrane-bound but, upon exposure to escherichia coli membrane vesicles or to liposomes prepared from e. coli lipids, it assembles into the bilayer in an integral fashion. much of this prot ... | 1978 | 273906 |
| a cascade mechanism of transcription in bacteriophage m13 dna. | | 1978 | 664236 |
| expression of bacteriophage m13 dna in vivo. localization of the transcription initiation and termination signal of the mrna coding for the major capsid protein. | during the infection cycle of the filamentous bacteriophage m13 a phage specific rna species is made which selectively directs in vitro the synthesis of the precursor of the major capsid protein encoded by gene viii. this rna is unstable (its mean half-life is 11 min) and is made in amounts representing at least 2% of the newly synthesized rn. nucleotide sequence analysis have indicated that the synthesis of this rna species is initiated and terminated at the same promoter (g0.18) and terminatio ... | 1978 | 693322 |
| cloning, nucleotide sequence, overexpression, and inactivation of the escherichia coli 2-keto-4-hydroxyglutarate aldolase gene. | having previously determined the complete amino acid sequence of 2-keto-4-hydroxyglutarate aldolase from escherichia coli (c. j. vlahos and e. e. dekker, j. biol. chem. 263:11683-11691, 1988), we amplified the gene that codes for this enzyme by the polymerase chain reaction using synthetic degenerate deoxyoligonucleotide primers. the amplified dna was sequenced by subcloning the polymerase chain reaction products into bacteriophage m13; the nucleotide sequence of the gene was found to be in exac ... | 1992 | 1339418 |
| replication of bacteriophage m13. xiii. structure and replication of cloned m13 miniphage. | | 1978 | 731688 |
| genomic variations in mosquitocidal strains of bacillus sphaericus detected by m13 dna fingerprinting. | the genomic variation of bacillus sphaericus reference and local strains belonging to different serotypes was examined by dna fingerprinting. a phage m13 dna probe detected a number of variable fragments in the restriction digests of total strain dnas. the patterns of band distribution showed a certain homology among mosquitocidal strains, expressed by similarity index d and might be a reliable criterion for assessing the level of genomic similarity between closely related strains. an important ... | 1992 | 1352319 |
| restriction-enzyme-cleavage maps of bacteriophage m13. existence of an intergenic region on the m13 genome. | replicative form dna of bacteriophage m13 was cleaved into specific fragments by an endonuclease isolated from hemophilus aegyptius (endor.haeii) and an endonuclease from arthrobacter luteus (endor.alui). the fragments were ordered as to construct a circular map of the phage m13 genome by: (a) using each fragment as a primer for the synthesis in vitro of its respective neighbour and (b) digesting the isolated fragments with the hemophilus aegyptius enzyme endor.haeii or the hemophilus aphirophil ... | 1976 | 786638 |
| transcription of bacteriophage m13 dna: existence of promoters directly preceding genes iii, vi, and i. | in vitro transcription and coupled transcription-translation studies have been performed with restriction fragments of bacteriophage m13 replicative-form dna which contain either gene iii, gene vi, or gene i. it could be demonstrated that dna fragments which contain gene iii were able to direct the synthesis of gene iii protein. fragments which encompassed genes vi and i gave rise to the synthesis of gene i protein only, whereas gene i-containing fragments were able to direct the synthesis of ge ... | 1978 | 731795 |
| fractionation of membrane vesicles from coliphage m13-infected escherichia coli. | membrane vesicles were prepared by osmotic lysis of spheroplasts from m13-infected escherichia coli. reduced nicotinamide adenine dinucleotide (nadh) oxidase (reduced nad: oxidoreductase, ec 1.6.99.3) and mg2+-ca2+-activated adenosine triphosphatase (atp phosphohydrolase, ec 3.6.1.3), which are normally localized to the inner surface of the cytoplasmic membrane, were 50% acceesible to their polar substrates in these vesicles. the major coat protein of coliphage m13 is also bound to the cytoplasm ... | 1976 | 132427 |
| the role of escherichia coli dnag function in coliphage m13 dna synthesis. | examination of the role of escherichia coli dnag function in different stages of m13 phage dna synthesis by ultracentrifugal analysis of intracellular phage dna in a thermosensitive dnag mutant shows that: (a) the formation of parental double-strand replicative-form dna (rfdna) from the infecting virus is independent of dnag function; (b) the synthesis of progeny rfdna requires dnag product; (c) after a pool of rfdna is made up, dnag function is not required for the progeny single-strand dna (ss ... | 1976 | 786625 |
| replication of bacteriophage m13. x. m13 replication in a mutant of escherichia coli defective in the 5' leads to 3' exonuclease associated with dna polymerase i. | | 1976 | 789894 |
| bacterial rep- mutations that block development of small dna bacteriophages late in infection. | several related mutants of escherichia coli c have been isolated that block the growth of the small icosahedral dna phages phix174 and s13 late in infection. phage g6 is also blocked, at a stage not yet known. growth of the filamentous phage m13, though not blocked, is affected in these strains. these host mutations co-transduce with ilv at high frequency, as do rep- mutations. however, the new mutants, designated grol-, differ from previously studied rep- mutants in that they permit synthesis o ... | 1976 | 789914 |
| mapping of promoter sites on the genome of bacteriophage m13. | with the aid of transcription studies on restriction fragments of bacteriophage m13 dna it has been demonstrated that at least eight promoter sites are located on the m13 genome. five of these promoters initiate the synthesis of rna chains which contain at their 5'-terminal end pppg (g promoters), while the other three promoters initiate rna chains which start exclusively with pppa (a promoters). the positions of these promoter sites on the physical map are: 0.82 (g0.82), 0.88 (g0.88), 0.94 (g0. ... | 1976 | 795656 |
| nucleotide sequence of the origin for bacteriophage m13 dna replication. | | 1979 | 289455 |
| role of hydrophobic forces in membrane protein asymmetry. | m13 virus coat protein is an integral cytoplasmic membrane protein at all stages of viral infection. the pure virus coat protein can also be incorporated into synthetic lecithin vesicles near the lipid-phase transition temperature (tm), spanning the bilayer with its n terminus exposed on the outside and its c-terminus inside (wickner, w. (1976), proc. natl. acad. sci. u.s.a. 73, 1159-1163). the assembly of coat protein into vesicles in this asymmetric fashion has a sharp maximum near the phase-t ... | 1977 | 836786 |
| asymmetric orientation of phage m13 coat protein in escherichia coli cytoplasmic membranes and in synthetic lipid vesicles. | at each stage of infection, the major coat protein of coliphage m13 binds to the e. coli cytoplasmic membrane with its antigenic site exposed to the cell exterior [wickner, w. (1975) proc. nat. acad. sci. usa 72, 4749-4753]. this antigenic site is now shown to be at the amino-terminus of the protein. the amino-terminus of m13 coat protein is also found exclusively on the outside of dilauroyl or dimyristoyl lecithin vesicles, formed with coat protein by the cholate dilution technique [racker, e., ... | 1976 | 772680 |
| iolation and charcterization of a dna-binding non-histone protein from tetrahymena pyriformis. | three proteins (a, b and c) that bind specifically to single-stranded dna have been isolated from the eukaryotic organism tetrahymena pyriformis. their molecular weights are 47 000, 41 000 and 32 000. the amino acid composition of the a protein indicates that it is a non-histone protein and sucrose gradient centrifugation shows that it binds to bacteriophage m13 dna and to oligo (dt)100 in a cooperative manner. the exonucleolytic degradation of oligo (dt)100 is prevented when it is bound to the ... | 1975 | 809059 |
| replication of bacteriophage m13 ix. requirement of the escherichia coli dnag function for m13 duplex dna replication. | temperature-shift experiments with an escherichia coli dnag strain indicate a requirement for the dnag function for m13 phage production only at an early stage of infection. mutant cells infected at nonpermissive temperature form the parental rf (ss leads to rf) but do not replicate further. a shift to nonpermissive temperature after infection inhibits rf leads to rf replication but not rf leads to ss synthesis. the synthesis of both strands of the duplex rf was inhibited equally after a tempera ... | 1975 | 1097735 |
| replication of bacteriophage m13. xi. localization of the origin for m13 single-strand synthesis. | | 1977 | 845943 |
| effects of bacteriophage m13 infection upon phospholipid and fatty acid compositions of escherichia coli. | escherichia coli k38 were grown and infected with wild type and amber mutants of bacteriophage m13 in the early log phase. lipid compositions of the infected and healthy cultures, grown under identical conditions, were determined 2 hr after infection. from the results, it was observed that total lipid and total phospholipid content remained nearly constant, suggesting that the cell membrane which contained the maximum phospholipids was not damaged by the infection. moreover, the percentage of di ... | 1975 | 1099382 |
| studies on dna unwinding. proton and phosphorus nuclear-magnetic-resonance studies of gene v protein from bacteriophage m13, interacting with d(pc-g-c-g). | the interaction of gene v protein from bacteriophage m13 with the self-complementary tetranucleotide d(pc-g-c-g) was studied by 1h and 31p nuclear magnetic resonance. it is shown, using the hydrogen-bonded proton resonances of the watson-crick base pairs as a probe, that the protein is able to unwind the small double-helical fragment even at 0 degrees c. binding of the tetranucleotide causes changes in the aromatic part of the 1h nmr spectrum of the complex, suggesting that aromatic residues, mo ... | 1977 | 598376 |
| identification and mapping of origins of dna replication within the dna sequences of the genome of insect iridescent virus type 6. | the origins of dna replication of the genome (209 kbp) of chilo iridescent virus (civ), which is circularly permuted and terminally redundant, were identified. the defined genomic library of civ, which represents 100% of dna sequences of the viral genome (e.g., all 32 ecori civ dna fragments), was used for transfection of choristoneura fumiferana insect cell cultures (cf-124) that were previously infected with civ. the plasmid rescue experiments were carried out to select those recombinant plasm ... | 1992 | 1549908 |
| physical mapping of the central terminator for transcription on the bacteriophage m13 genome. | with the aid of in vitro transcription and translation studies it has been demonstrated that termination of transcription on bacteriophage m13 replicative form dna occurs at a unique site which is located immediately distal to the 3'-end of gene viii, the gene which codes for the major capsid protein. the position of this site has been mapped accurately on the enzyme cleavage maps by transcription of restriction fragments of m13 rf dna. the central termination site was found to be located in res ... | 1975 | 1103087 |
| studies on bacteriophage m13 dna. 1. a cleavage map of the m13 genome. | a physical map of the bacteriophage m13 genome has been constructed on the basis of specific cleavage of m13 replicative form dna by bacterial restriction endonucleases. the 13 fragments produced by the enzyme from haemophilus aphirophilus (endonuclease r.hap ii) as well as the 10 fragments produced by the enzyme from haemophilus aegyptius (endonuclease r.hae iii) have been ordered by analysis of partial digest products and by analysis of overlapping sets of fragments. in addition, the single si ... | 1975 | 1079769 |
| studies on the structure of replicative intermediates in bacteriophage m13 single stranded dna synthesis. | pulse-labeled replicative intermediates in m 13 single stranded dna synthesis can be separated by dye-buoyant density centrifugation into two major fractions: supercoiled molecules (ri i) containing viral strands of more than one genome length, and "relaxed" molecules (ri ii) with labeled dna chains shorter than unit length. it is postulated that ri ii molecules might be formed in vivo by site-specific nicking of rf i molecules. | 1975 | 1129143 |
| electron microscopic studies of bacteriophage m13 dna replication. | intracellular forms of m13 phage dna isolated after infection of escherichia coli with wild-type phage have been studied by electron microscopy and ultracentrifugation. the data indicate the involvement of rolling-circle intermediates in single-stranded dna synthesis. in addition to single-stranded circular dna, we observed covalently closed and nicked replicative-form (rf) dnas, dimer rf dnas, concatenated rf dnas, rf dnas with single-stranded tails (theta, rolling circles), and, occasionally, ... | 1977 | 916032 |
| miniphage-a class of satellite phage to m13. | satellite or defective bacteriophage particles can appear in extensively recycled stocks of coliphage m13. these particles, herein known as miniphage, replicate using the wild type bacteriophage as a helper. their physical properties (u.v. spectra, sedimentation of dna and bacterophage, electrophoretic moblitiy) are described and a method for the isolation of specific satellite bacteriophage is presented. | 1975 | 1123611 |
| stoichiometry, selectivity, and exchange dynamics of lipid-protein interaction with bacteriophage m13 coat protein studied by spin label electron spin resonance. effects of protein secondary structure. | bacteriophage m13 major coat protein has been isolated with cholate and reconstituted in dimyristoyl- and dioleoylphosphatidylcholine (dmpc and dopc, respectively) bilayers by dialysis. fourier transform infrared spectra of dmpc/coat protein recombinants confirmed that, whereas the protein isolated by phenol extraction was predominantly in a beta-sheet conformation, the cholate-isolated coat protein contained a higher proportion of the alpha-helical conformation [cf. spruijt, r. b., wolfs, c. j. ... | 1992 | 1312343 |
| [preparation of single-stranded e1a-oncogene dna fragments from simian adenovirus sa7 by endonuclease hydrolysis recombinant phage m13 ss-dna complexed with oligonucleotides, containing restriction sites]. | the ss-dna of the (+) and (-) chains of ela dna fragment was obtained by hydrolysis of the recombinant bacteriophages m13 mp8g and mp9g (where g is 1-1750 bp:, e1a region of oncogene sa7) in complexes with the 16 bp oligonucleotides containing alui and bspri sites of restriction and sequences complementary to e1a sa7. the obtained fragments overlap the e1a zones associated with the immortalizing potential of sa7. | 1992 | 1301501 |
| regulation of gene activity in bacteriophage m13 dna: coupled transcription and translation of purified genes and gene-fragments. | | 1975 | 1189286 |
| corrected nucleotide sequence of m13mp18 gene iii. | there are seven differences between the actual nucleotide (nt) sequence of bacteriophage m13mp18 gene iii and the previously reported nt sequence (which had been compiled based on the nt sequence of wild-type bacteriophage m13 gene iii). | 1992 | 1375182 |
| functional half-lives of bacteriophage m13 gene 5 and gene 8 messages. | the half-lives of the m13 gene 5 and gene 8 messages were determined by measuring the decay in the rate of synthesis of the gene 5 and gene 8 proteins after inhibition of new rna chain initiations with rifampin. the gene 5 and gene 8 messages decay with half-lives of approximately 2.5 and 5 min, respectively. we found no evidence of a functional m13 message with a half-life as long as that reported for hybridizable mrna. | 1976 | 1255878 |
| cleavage maps of the filamentous bacteriophages m13, fd, fl, and zj/2. | the replicative form dnas of bacteriophage m13, fd, f1, and zj/2 were found to be sensitive to cleavage by the restriction endonucleases endor-hapii, endor-haeii, endor-haeiii, endor-hindii, endor-alui, endor-hha, and endor-hinf. with respect to m13 dna the number of cleavage sites varied from 21 for endor-hinf, 18 for endor-alui, 15 for endor-hha, 13 for endor-hapii, 10 for endor-haeiii, 3 for endor-haeii, to only a single site for endor-hindii. in contrast to m13, fd and f1, the zj/2 dna molec ... | 1976 | 1271528 |
| spectroscopy of lipid-protein interactions: structural aspects of two different forms of the coat protein of bacteriophage m13 incorporated in model membranes. | | 1992 | 1287668 |
| a bacteriophage m13 based sandwich hybridization assay for the detection of hepatitis b virus in human blood. | a two step hybridization procedure was developed to detect the presence of hepatitis b virus in blood samples using bacteriophage m13 radiolabelled dna as probe. during the first step of hybridization, single-stranded bacteriophage m13 tg 130 dna, with 3.2 kb hbv dna cloned into it, was hybridized to target hbv dna immobilized on nitrocellulose membrane filter. in the second step of hybridization, m13 dna annealed to hbv target is detected with the help of double stranded form of m13 dna. the as ... | 1991 | 1652551 |
| formation of non-bilayer structures induced by m13 coat protein depends on the conformation of the protein. | a comparison is made of the interaction of the coat protein of bacteriophage m13 in a predominant alpha-helix conformation and in a predominant beta-sheet conformation. to perform a systematic study of the interaction between the protein in these two different forms of the surrounding lipid matrix, nmr spectra of 2h-nuclei of specific labelled phospholipid systems are measured. in addition 31p-nmr is employed to provide information about the morphological structure adopted by the reconstituted l ... | 1992 | 1390851 |
| protease inhibitor display m13 phage: selection of high-affinity neutrophil elastase inhibitors. | we report display of the complete protease inhibitor (kunitz) domain, bpti, on the surface of bacteriophage m13 as a fusion to the gene iii product. phage that display bpti bind specifically to anti-bpti antibodies, trypsin and anhydrotrypsin. a point mutation of bpti [lys15-->leu(k15l)] alters the binding specificity of fusion phage such that a human neutrophil elastase-binding phenotype is conferred while a trypsin-binding phenotype is eliminated. phage were eluted from an immobilized protease ... | 1992 | 1385268 |
| fab assembly and enrichment in a monovalent phage display system. | we have developed a system that allows the expression of a single copy of an antibody fab molecule on the surface of the filamentous bacteriophage m13 and demonstrate the utility of this system for enrichment of specific "fab phage". a "humanized" version of antibody 4d5 (h4d5) directed against the extracellular domain of the her2 (neu) receptor, was used as prototype to assess the assembly of fab molecules on the phage and to determine the power of the enrichment system. the h4d5 fab phage show ... | 1991 | 1369462 |
| determination of twin zygosity by dna hybridisation to wild type bacteriophage m13. | | 1992 | 1420002 |
| typing of methicillin-resistant staphylococcus aureus with an m13 repeat probe. | a bacteriophage m13 tandem repeat has been used to probe ecori digested genomic dna of methicillin-resistant staphylococcus aureus (mrsa). the patterns generated were found to be useful in typing mrsa and generally confirmed the relationships that had previously been recognized in other studies based on antimicrobial resistance and plasmid profiles. the epidemic mrsa of london hospitals (emrsa) and the majority of the epidemic mrsa of eastern australian hospitals (ea mrsa) gave the same pattern. ... | 1992 | 1350600 |
| identification and characterization of the in vitro synthesized gene products of bacteriophage m13. | bacteriophage m13 replicative form (rf) dna was used to direct coupled transcription and translation in cell-free extracts prepared from escherichia coli. by using rf dna, isolated from cells infected with appropriate amber mutants of this phage, it has been possible to identify the products of genes i through iv. by using the same methods no gene-product relationship could be demonstrated for genes vi and vii. coupled in vitro protein synthesis studies on rf-iii dna, a linear double-stranded dn ... | 1975 | 1089807 |
| ten proteins required for conversion of phix174 single-stranded dna to duplex form in vitro. resolution and reconstitution. | protein requirements for conversion of phix174 single-stranded dna to a double-stranded replicative form with a small gap (rf ii) have been determined by resolution and reconstitution of the multienzyme system from extracts of gently lysed escherichia coli. assays depended on: (a) complementation of extracts of thermosensitive mutants and (b) fractionation of extracts of wild type cells to divide essential components into groups, each of which was further resolved. these procedures have yielded ... | 1975 | 1097445 |
| transmembrane region of wild-type and mutant m13 coat proteins. conformational role of beta-branched residues. | although transmembrane (tm) segments of integral membrane proteins are putatively alpha-helical in conformation, beta-sheet promoters (val, ile, thr) often account for approximately 40% of tm residue composition. we are examining the conformational role(s) of these residues, using as a model system the major coat protein of the filamentous bacteriophage m13. this 50-residue protein, which is located at the escherichia coli host membrane during phage reproduction, contains a prototypic 19-residue ... | 1992 | 1544912 |
| selection and characterization of randomly produced mutants of gene v protein of bacteriophage m13. | gene v protein of bacteriophage ff (m13, f1, fd) is a master regulator of phage dna replication and phage mrna translation. it exerts these two functions by binding to single-stranded viral dna or to specific sequences in the 5' ends of its target mrnas, respectively. to study the structure/function relationship of gene v protein, m13 gene v was inserted in a phagemid expression vector and a library of missense and nonsense mutants was constructed by random chemical mutagenesis. phagemids encodi ... | 1992 | 1551382 |
| purification and properties of dna polymerase from bacillus caldotenax. | a thermostable dna polymerase was prepared from bacillus caldotenax by using a four-step chromatography procedure. the protein exists as a monomer of m(r) 94,000, has a pi of 4.9 and has no associated 3'-5' or 5'-3'-exonuclease activities or endonuclease activity. the temperature optimum of the enzyme was about 70 degrees c and the ph for maximum activity was about 7.5. the enzyme has an absolute requirement for a bivalent cation, and maximum activity was obtained at the unusually high concentra ... | 1992 | 1445254 |
| studies on bacteriophage m13 dna. 2. the gene order of the m13 genome. | the double-stranded replicative form dna of bacteriophage m13 was cleaved into 13 specific fragments by the restriction endonuclease from haemophilus aphirophilus. the individual dna fragments from wild-type replicative form molecules were then annealed to circular, single-stranded dnas of phage m13, bearing amber mutations as genetic markers. when such dna hybrids infected competent escherichia coli cells, only those duplexes which were genetically heterozygous gave rise to wild-type phages in ... | 1975 | 1095371 |
| identification of potential active-site residues in the escherichia coli leader peptidase. | leader peptidase of escherichia coli cleaves the leader sequence from the amino terminus of membrane and secreted proteins after these proteins insert across the membrane. despite considerable research, the mechanism of catalysis of leader peptidase remains unknown. this peptidase cannot be classified using protease inhibitors to the serine, cysteine, aspartic acid, or metallo- classes of proteases (zwizinski, c., date, t., and wickner, w. (1981) j. biol. chem. 256, 3593-3597). using site-direct ... | 1992 | 1618816 |
| dna transcription and repressor binding affect deletion formation in escherichia coli plasmids. | chimeric plasmids containing phage m13 and plasmid pbr322 sequences undergo deletions in escherichia coli with a high frequency. in all plasmids one deletion endpoint is the m13 replication origin nick site. we examined the effects of transcription on the position of the other deletion end-point, by inserting in the plasmids an inducible promoter followed by a transcription terminator. transcription dramatically affected deletions in an orientation-dependent way, such that greater than 95% of en ... | 1992 | 1396563 |
| assignment of amide 1h and 15n nmr resonances in detergent-solubilized m13 coat protein: a model for the coat protein dimer. | the major coat protein of the filamentous coliphage m13 is a 50-residue integral membrane protein. detergent-solubilized m13 coat protein is a promising candidate for structure determination by nuclear magnetic resonance methods as the protein can be prepared in large quantities and the protein-containing micelle is reasonably small. under the conditions of our experiments, sds-bound coat protein exists as a dimer with an apparent molecular weight of 27,000. broad lines and poor resolution in th ... | 1992 | 1606152 |
| using the polymerase chain reaction to maintain dna probe inventories in clinical and diagnostic laboratories. | clinical and diagnostic dna laboratories must maintain a large inventory of dna probes for use in hybridization studies. the preparation of plasmid dna and isolation of dna fragments for use as probes in both expensive and time consuming. we present here a rapid and relatively inexpensive method of producing large amounts of dna fragments from stocks, using the polymerase chain reaction (pcr). our experience over the past year using this technique has been very positive and we believe many labor ... | 1991 | 1673093 |
| fluorescence studies of the binding of bacteriophage m13 gene v mutant proteins to polynucleotides. | this investigation describes how the binding characteristics of the single-stranded dna-binding protein encoded by gene v of bacteriophage m13, are affected by single-site amino acid substitutions. the series of mutant proteins tested includes mutations in the purported monomer-monomer interaction region as well as mutations in the dna-binding domain at positions which are thought to be functionally involved in monomer-monomer interaction or single-stranded dna binding. the characteristics of th ... | 1992 | 1606950 |
| the nucleotide sequence of 3c proteinase region of the coxsackievirus a24 variant: comparison of the isolates in taiwan in 1985-1988. | acute hemorrhagic conjunctivitis caused by coxsackievirus a24 variant (ca24v) first appeared in taiwan in october 1985, followed by two other sequential epidemics in 1986 and 1988. in order to know the evolutionary relationship of the ca24v strains isolated in taiwan, we first determined the nucleotide sequence of the 3c proteinase (3cpro) region of the prototype strain (eh24/70), isolated in singapore in 1970, by molecular cloning. the nucleotide sequence of the 3cpro region thus sequenced show ... | 1991 | 1647565 |
| survival of phage m13 with uracils on one or both dna strands. | the survival of m13 dna was studied after partial replacement of thymine by uracil in the bacteriophage. uracils carry the same genetic information as the thymines. nevertheless in escherichia coli wild-type cells, uracils in dna are replaced by thymines by excision repair initiated by uracil-dna glycosylase (udg). thus inactivation of uracil-containing phage dna is solely due to repair initiated by udg. incorporation of uracils was achieved in one or in both strands, either randomly or site-spe ... | 1992 | 1620092 |
| three-dimensional structure of a cloning vector. x-ray diffraction studies of filamentous bacteriophage m13 at 7 a resolution. | filamentous bacteriophage m13 is a single-stranded dna phage about 65 a in diameter and 9300 a long. x-ray diffraction studies of magnetically oriented fibers of native, mercury and iodine-labeled phage particles have been used to determine the arrangement of the major coat protein, the gene 8 product, in the virion. the coat protein is made up of a single gently curving alpha-helix extending from approximately pro6 to near the carboxyl terminus. the axis of the alpha-helix is tilted about 20 de ... | 1992 | 1640460 |
| two regions of m13 phage genome hybridizing with human dna are similar to several keratin genes. | dna from two regions of the phage m13 genome hybridizes with dna restriction fragments from genomes of various species including man [15, 20]. as the pattern of hybridization is individual-specific, this phage m13 probe can be used for dna fingerprinting. we demonstrate here that the regions of many keratin genes coding for glycine-rich parts of c and n end domains are very similar to the phage m13 probe, and this similarity may be responsible for hybridization. | 1990 | 1710508 |
| hepatitis b surface antigen particles with all four subtypic determinants: point mutations of hepatitis b virus dna inducing phenotypic changes or double infection with viruses of different subtypes. | hepatitis b surface antigen (hbsag) particles carry the common determinant, a, as well as d or y and w or r subtype determinants, and are classified into the four major subtypes, i.e., adw, adr, ayw and ayr. rare sera contain hbsag particles with all four subtype determinants (adywr). target sequences (nucleotides 38-550) in the s gene of hepatitis b virus (hbv) dna in two such sera were amplified by the polymerase chain reaction. individual amplification products were cloned in an m13 phage vec ... | 1990 | 1694959 |
| preferential transposition of an is630-associated composite transposon to ta in the 5'-ctag-3' sequence. | a composite transposon, tn4731, associated with is630 has been shown to transpose preferentially to 5'-ta-3' sequences that are located at two sites in a rho-dependent transcription terminator in plasmid cole1 in escherichia coli (t. tenzen, s. matsutani, and e. ohtsubo, j. bacteriol. 172:3830-3836, 1990). here we demonstrated that tn4731 preferentially transposes to ta sequences at four sites in plasmid puc118 and its derivatives: the ta sequence (hot spot i) in the intergenic region of phage m ... | 1991 | 1655702 |
| hormone phage: an enrichment method for variant proteins with altered binding properties. | human growth hormone (hgh), a 191 residue protein containing two disulfide bonds, was fused to the carboxyl-terminal domain of the gene iii protein, a minor coat protein exposed at one end of the filamentous phage m13. the gene fusion was cloned into a plasmid containing origins of replication for escherichia coli and filamentous phage and was packaged into phagemid particles upon infection by an m13ko7 helper phage. transcription of the hgh-gene iii fusion was controlled so that usually no more ... | 1990 | 1708882 |
| synthesis of dna by human immunodeficiency virus reverse transcriptase is preferentially blocked at template oligo(deoxyadenosine) tracts. | the genome of human immunodeficiency virus (hiv) and especially the envelope gene are mutated with unusually high frequency during in vivo replication. recent studies indicate that hiv reverse transcriptase (rt) is unusually error prone and that the number of generated mutations is disproportionately high within repetitive base sequences. to study the ability of recombinant and wild-type hiv rt to traverse specific homo-oligomeric stretches, we used bacteriophage m13 dna templates that contain d ... | 1990 | 1698789 |
| [rna and dna synthesis catalyzed by a dna-polymerase alpha complex with primase from silkworm cells on single-stranded dna]. | depending on the ionic environment the replicative complex of silkworm bombyx mori, containing dna polymerase alpha and primase, catalyzes on single-stranded dna of phage m13 a ntp-dependent synthesis or elongation of preformed primers. in the presence of ntps and dntps at conditions optimal for the ntp-dependent synthesis the replicative complex synthesizes on m13 dna oligoribonucleotides of 9-11 residues, which serve as primers for polymerization of dna. the length of rna-primers synthesized b ... | 1991 | 1716736 |
| terminating a macromolecular helix. structural model for the minor proteins of bacteriophage m13. | analysis of the results of x-ray diffraction, electron microscopy and s sequence studies of filamentous bacteriophage m13 are used to construct structural models for the minor proteins gp7 and gp9 at the end of the virus assembled first, and a portion of gp6 at the end of the virus that binds host. comparison of the sequence of the major coat protein, gp8, with those of gp7, gp9 and gp6 indicates that significant portions of these three proteins have sequences similar to that of gp8. assuming th ... | 1992 | 1469721 |
| identification of meningococcal serosubtypes by polymerase chain reaction. | the polymerase chain reaction was used as the basis of a novel typing method for neisseria meningitidis. southern hybridization experiments demonstrated that it was possible to identify genes encoding different serological variants of the meningococcal class 1 outer membrane protein by probing with polymerase chain reaction products corresponding to known epitopes. a set of 14 defined variable regions was prepared in bacteriophage m13mp19 by the cloning of polymerase chain reaction products. the ... | 1992 | 1452652 |
| genetic effects of oxidative dna damage: comparative mutagenesis of 7,8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine in escherichia coli. | a single 7,8-dihydro-8-oxoguanine (g8-oxo; 8-hydroxyguanine) adduct in the lacz alpha gene of bacteriophage m13 dna induces a targeted g-->t transversion after replication in escherichia coli (biochemistry, 29, 7024-7031 (1990)). this mutation is thought to be due to the facile formation during dna synthesis of a g8-oxo.base pair, where g8-oxo is in the syn conformation about the deoxyglycosyl bond. a related modified purine, 7,8-dihydro-8-oxoadenine (a8-oxo; 8-hydroxyadenine), is an abundant pr ... | 1992 | 1461734 |
| [study of the mutagenic properties of oligonucleotides containing and internucleotide pyrophosphate bond using a mutation system based on phage m13]. | mutagenic properties of oligonucleotides with pyrophosphate internucleotide bond was studied. it was shown that the pyrophosphate bond in the oligo structure does not induce mutations but promotes a more efficient induction of marker deletions predetermined by the nucleotide sequence as compared to the native oligonucleotide. marker deletion induction proceeds according to the repair mechanism as homozygotes dominate in the mutant generation. | 1991 | 1667540 |
| deletions in the trna(lys) primer-binding site of human immunodeficiency virus type 1 identify essential regions for reverse transcription. | the initiation of human immunodeficiency virus type 1 (hiv-1) reverse transcription occurs by the extension of a trna primer bound near the 5' end of the genomic rna at a position termed the primer-binding site (pbs). the pbs is an 18-nucleotide region of the hiv-1 genome complementary to cellular trna(lys). to further investigate the sequence requirements for the pbs in reverse transcription, deletions in the pbs were created and subcloned into a plasmid containing the infectious hiv-1 proviral ... | 1991 | 1714513 |
| role of dna polymerase 3'----5' exonuclease activity in the bypass of aminofluorene lesions in dna. | n-(deoxyguanosin-8-yl)-2-(acetylamino)fluorene (aaf-g) adducts in the dna of bacteriophage m13 can be converted to n-(deoxyguanosin-8-yl)-2-aminofluorene (af-g) adducts in situ by treatment with 1.0 m naoh for 45 min at room temperature. the conversion is accompanied by a dramatic increase in the transfection activity of the samples which is correlated with the measured deacetylation of the acetylaminofluorene adduct. the pair of substrates (aaf-g/af-g) with adducts at identical places in the dn ... | 1990 | 1702368 |
| experience in shotgun sequencing a 134 kilobase pair dna molecule. | until now, large dna sequences have been obtained by cloning fragments of the target molecule into plasmid, cosmid or bacteriophage lambda vectors. the 134 kbp dna sequence of channel catfish virus was determined with relative ease by shotgun cloning of random fragments of genomic dna directly into a bacteriophage m13 vector, sequencing by dideoxynucleotide chain termination, and compilation of the data using staden's database handling programs. experience gained during this endeavour indicates ... | 1991 | 1768862 |
| assignment of the 1h nmr spectrum and secondary structure elucidation of the single-stranded dna binding protein encoded by the filamentous bacteriophage ike. | by means of 2d nmr techniques, all backbone resonances in the 1h nmr spectrum of the single-stranded dna binding protein encoded by gene v of the filamentous phage ike have been assigned sequence specifically (at ph 4.6, t = 298 k). in addition, a major part of the side chain resonances could be assigned as well. analysis of noesy data permitted the elucidation of the secondary structure of ike gene v protein. the major part of this secondary structure is present as an antiparallel beta-sheet, i ... | 1992 | 1734970 |
| gene v protein-mediated translational regulation of the synthesis of gene ii protein of the filamentous bacteriophage m13: a dispensable function of the filamentous-phage genome. | introduction of a deletion in the genome of wild-type m13 bacteriophage that eliminates translational repression of m13 gene ii by its cognate gene v protein had no effect on phage viability. furthermore, it was noted that gene v protein of phage ike, a distant relative of m13, does not function as a translational repressor of its cognate gene ii protein. the data strongly indicate that the gene v protein-mediated control of gene ii expression in bacteriophage m13 is an evolutionary relic of the ... | 1992 | 1729248 |
| viable transmembrane region mutants of bacteriophage m13 coat protein prepared by site-directed mutagenesis. | bacteriophage m13 coat protein - a 50-residue protein located at the e. coli host membrane during phage reproduction - is subjected to cytoplasmic, membrane-bound, and dna-interactive environments during the phage life cycle. in research to examine the specific features of primary/secondary structure in the effective transmembrane (tm) region of the protein (residues 21-39: yigyawamvvvivgatigi) which modulate its capacity to respond conformationally to the progressive influences of these varying ... | 1991 | 1953741 |
| genetic assay of misincorporation. | a system to characterize mutations arising from in vitro nucleotide misincorporation, which avoids the effects of in vivo mismatch repair on recovery of mutants, was constructed and evaluated. the laci gene of escherichia coli was inserted into phage m13 and the m13-laci recombinant was introduced into a strain of e. coli lacking a resident laci gene. in this system the function of the m13-bearing laci gene can be detected by plaque color. mutants in the 5'-region of the laci gene (encoding oper ... | 1991 | 1720870 |
| inserting foreign peptides into the major coat protein of bacteriophage m13. | foreign dna fragments were inserted into filamentous phage gene viii to create hybrid b-proteins with foreign sequences in the amino terminus. the hybrid proteins are incorporated into the virions which retain viability and infectivity. virions with hybrid b-proteins have the same contour length and the same number of b-protein molecules as virions with natural b-proteins. it was shown that for one of hybrid b-proteins the position of the processing site had changed. | 1992 | 1577174 |