| infection of phaseolus vulgaris by bean golden mosaic virus: ultrastructural aspects. | | 1978 | 685179 |
| infectivity and complete nucleotide sequence of the cloned genomic components of a bipartite squash leaf curl geminivirus with a broad host range phenotype. | through cloning and molecular analysis we have identified two highly homologous bipartite geminiviruses as causing squash leaf curl disease. mechanical and agrobacterium-mediated inoculation of plants with cloned viral dna components identified the two genomic components of sqlcv-e, a squash leaf curl virus with an unexpectedly broad host range for a whitefly-transmitted geminivirus. nucleotide sequence analysis of the genome of this virus showed it to have the same bipartite component organizat ... | 1991 | 1984668 |
| total nucleotide sequences of the infectious cloned dnas of bean golden mosaic virus. | complete nucleotide sequences of the infectious cloned dna components (dna a and b) of bean bolden mosaic virus were determined. the dna a (2585 nucleotides) and dna b (2647 nucleotides) have little sequence homology with each other, but both a and b contain a common region of 205 nucleotides. a possible large hairpin structure is detected in the common region. nucleotide sequences of dnas a and b revealed the presence of 8 potential coding regions for proteins (m.w. greater than 10,000). among ... | 1987 | 3037283 |
| the nucleotide sequence and genome structure of mung bean yellow mosaic geminivirus. | complete nucleotide sequences of the infectious cloned dna components (dna 1 and dna 2) of mung bean yellow mosaic virus (mymv) were determined. mymv dna 1 and dna 2 consists of 2,723 and 2,675 nucleotides respectively. dna 1 and dna 2 have little sequence similarity except for a region of approximately 200 bases which is almost identical in the two molecules. analysis of open reading frames revealed nine potential coding regions for proteins of mol. wt. > 10,000, six in dna 1 and three in dna 2 ... | 1993 | 8231962 |
| host and viral factors determine the dispensability of coat protein for bipartite geminivirus systemic movement. | geminiviruses have unique, twinned icosahedral particles which encapsidate circular single-stranded dna. their genomes are composed of either one or two dna segments. monopartite geminiviruses absolutely require a functional coat protein (cp) for infectivity, whereas bipartite geminivirus cp null mutants can infect plants systemically. however, we show here that a cp mutant of the bipartite tomato golden mosaic virus (tgmv), which can infect nicotiana benthamiana systemically, is confined to the ... | 1996 | 8615033 |
| two domains of the al1 protein mediate geminivirus origin recognition. | the geminiviruses tomato golden mosaic virus (tgmv) and bean golden mosaic virus (bgmv) have bipartite genomes. their a and b dna components contain cis-acting sequences that function as origins of replication, while their a components encode the trans-acting replication proteins--al1 and al3. earlier experiments demonstrated that virus-specific interactions between the cis- and trans-acting functions are required for tgmv and bgmv replication and that the al1 proteins of the two viruses specifi ... | 1997 | 9426458 |
| genetic determinants of host-specificity in bipartite geminivirus dna a components. | geminiviruses are small, ssdna-containing plant viruses. bean golden mosaic virus (bgmv) and tomato golden mosaic virus (tgmv) have bipartite genomes, the components of which are designated a and b. although they are closely related, bgmv and tgmv nevertheless exhibit distinct host-specific phenotypes, with bgmv being well adapted to beans and tgmv being well adapted to nicotiana benthamiana. a previous study showed that the two open reading frames (orfs) of dna b only partially determine the ho ... | 1998 | 9837800 |
| genetic analysis of bipartite geminivirus tissue tropism. | the bipartite geminiviruses bean golden mosaic virus (bgmv), cabbage leaf curl virus (cablcv), and tomato golden mosaic virus (tgmv) exhibit differential tissue tropism in nicotiana benthamiana. in systemically infected leaves, bgmv remains largely confined to vascular-associated cells (phloem-limited), whereas cablcv and tgmv can escape into the surrounding mesophyll. previous work established that tgmv bri, the noncoding region upstream from the br1 open reading frame (orf), is required for me ... | 2001 | 11878900 |
| restriction map and southern analysis of the bean golden mosaic virus genome. | a restriction map showing 5'-3' polarity for two species of circular single-stranded (ss) dna from bean golden mosaic virus (bgmv) is presented. the two viral single-stranded dnas, which are very similar in size, were separated by prolonged agarose gel electrophoresis at low voltage. hybridization analysis using probes made from these separated dnas shows they are largely dissimilar in nucleotide sequence except for a common region of high homology which has been mapped to a unique location on e ... | 1983 | 18639119 |
| functional equivalence of late gene promoters in bean golden mosaic virus with those in tomato golden mosaic virus. | in the bipartite geminivirus tomato golden mosaic virus (tgmv), the activity of late gene promoters is up-regulated by the multifunctional viral protein al2. cis-acting sequences required for al2-mediated promoter responses have not been well characterized. however, nucleotide sequence analysis has implicated a motif termed the conserved late element (cle). the cle is present in tgmv and many other begomoviruses, although it is not ubiquitous. here we analysed the regulation of late gene express ... | 2001 | 11172109 |
| backcross assisted by microsatellite markers in common bean. | the objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. four populations were developed using donor parents resistant to the bean golden mosaic virus and, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. for crosses arc100-4 x dor303 and arc100-4 x phas8328, assisted selection was carried out in the f(2) and f(2)bc(1) generations, while ... | 2008 | 19048479 |
| three distinct begomoviruses associated with soybean in central brazil. | we report the complete nucleotide sequences of geminiviruses of the genus begomovirus infecting soybean (glycine max) in central brazil. samples obtained from soybean plants collected at santo antonio de goiás, goiás state, showing typical symptoms of viral infection, were analyzed. infection was confirmed by pcr-based amplification of a dna-a fragment with universal begomovirus primers. total dna from infected plants was then subjected to rolling-circle amplification (rca), and 2.6-kb molecules ... | 2009 | 19636495 |
| tissue specificity of geminivirus infection is genetically determined. | the types of cells and tissues infected by a virus define its tissue tropism. determinants of tissue tropism in animal-infecting viruses have been extensively investigated, but little is known about plant viruses in this regard. some geminiviruses in the genus begomovirus exhibit phloem limitation and are restricted to cells of the vascular system, whereas others can invade mesophyll tissue. to identify viral genetic determinants of tissue tropism, we established a model system using two begomov ... | 2000 | 11090223 |
| reduction of viral load in whitefly (bemisia tabaci gen.) feeding on rnai-mediated bean golden mosaic virus resistant transgenic bean plants. | the rnai concept was explored to silence the rep gene from the bean golden mosaic virus (bgmv) and a genetically modified (gm) bean immune to the virus was previously generated. we investigated if bgmv-viruliferous whiteflies would reduce viral amount after feeding on gm plants. bgmv dna amount was significantly reduced in whiteflies feeding in gm-plants (compared with insects feeding on non-gm plants) for a period of 4 and 8 days in 52% and 84% respectively. | 2015 | 26297125 |
| bipartite geminivirus host adaptation determined cooperatively by coding and noncoding sequences of the genome. | bipartite geminiviruses are small, plant-infecting viruses with genomes composed of circular, single-stranded dna molecules, designated a and b. although they are closely related genetically, individual bipartite geminiviruses frequently exhibit host-specific adaptation. two such viruses are bean golden mosaic virus (bgmv) and tomato golden mosaic virus (tgmv), which are well adapted to common bean (phaseolus vulgaris) and nicotiana benthamiana, respectively. in previous studies, partial host ad ... | 2000 | 11080490 |
| effect of silver reflective mulch and a summer squash trap crop on densities of immature bemisia argentifolii (homoptera: aleyrodidae) on organic bean. | polyethylene mulch with a reflective silver stripe and a yellow summer squash, cucurbita pepo l., trap crop were tested alone and in combination as tactics to reduce densities of bemisia argentifolii bellows & perring eggs and nymphs, and incidence of bean golden mosaic geminivirus on snap bean, phaseolus vulgaris l. egg densities were consistently higher on squash than on bean, but egg densities and virus incidence were not lower on bean grown with squash than on bean grown in monoculture. silv ... | 2000 | 10902322 |
| mutational analysis of the putative nicking motif in the replication-associated protein (ac1) of bean golden mosaic geminivirus. | geminiviruses are circular single-stranded dna viruses that replicate by a rolling circle mechanism involving the viral-encoded ac1 protein. dna nicking is necessary both for initiating replication of the covalently closed double-stranded dna templates and for releasing unit-length monomers. the effects of mutations in a putative nicking motif (k101 a y i d k106; e. v. koonin and t. v. ilyina, j. gen. virol. 73:2763-2766, 1992) of the ac1-derived protein for bean golden mosaic geminivirus isolat ... | 1996 | 8810074 |
| interactions between geminivirus replication proteins. | geminiviruses are small dna viruses that replicate in the nuclei of infected plant cells. the closely related geminiviruses tomato golden mosaic virus and bean golden mosaic virus each encode a protein, al1, that catalyzes the initiation of rolling-circle replication. both viruses also specify a second replication protein, al3, that greatly enhances the level of viral dna accumulation. using recombinant proteins produced in a baculovirus expression system, we showed that al1 copurifies with a pr ... | 1996 | 8794317 |
| populational survey of arthropods on transgenic common bean expressing the rep gene from bean golden mosaic virus. | genetically modified (gm) crops is considered the fastest adopted crop technology in the history of modern agriculture. however, possible undesirable and unintended effects must be considered during the research steps toward development of a commercial product. in this report we evaluated effects of a common bean virus resistant line on arthropod populations, considered as non-target organisms. this gm bean line (named m1/4) was modified for resistance against bean golden mosaic virus (bgmv) by ... | 2017 | 24922280 |
| virus and host-specific adaptations in the bl1 and br1 genes of bipartite geminiviruses. | the host range of individual geminiviruses may be quite narrow, and closely related viruses can exhibit distinct host adaptations. two such bipartite geminiviruses are bean golden mosaic virus (gbmv) and tomato golden mosaic virus (tgmv). in both, the bl1 and br1 genes are required for the spread of virus infection in plants. we have investigated the contributions of bl1 and br1 to host-specific phenotypes of bgmv and tgmv by constructing hybrid viruses in which these coding regions were exchang ... | 1995 | 8553533 |
| geminivirus replication origins have a modular organization. | tomato golden mosaic virus (tgmv) and bean golden mosaic virus (bgmv) are closely related geminiviruses with bipartite genomes. the a and b dna components of each virus have cis-acting sequences necessary for replication, and their a components encode trans-acting factors are required for this process. we showed that virus-specific interactions between the cis- and trans-acting functions are required for tgmv and bgmv replication in tobacco protoplasts. we also demonstrated that, similar to the ... | 1994 | 8180499 |
| whitefly transmission and efficient ssdna accumulation of bean golden mosaic geminivirus require functional coat protein. | bean golden mosaic geminivirus (bgmv) has a bipartite genome composed of two circular ssdna components (dna-a and dna-b) and is transmitted by the whitefly, bemisia tabaci. dna-a encodes the viral replication proteins and the coat protein. to determine the role of bgmv coat protein systemic infection and whitefly transmission, two deletions and a restriction fragment inversion were introduced into the bgmv coat protein gene. all three coat protein mutants produced systemic infections when coinoc ... | 1994 | 8091659 |
| mutational analysis of a putative ntp-binding domain in the replication-associated protein (ac1) of bean golden mosaic geminivirus. | bean golden mosaic virus (bgmv) is a whitefly-transmitted, ssdna geminivirus with a bipartite genome. ac1 is the only orf required for geminiviral replication. a putative ntp-binding motif, egx4gktx32dd, was present in the derived amino acid sequence of the replication-associated protein from the ac1 orf for 13 geminiviruses including bgmv-ga (guatemalan isolate, amino acids 221-263). we analyzed the phenotypes of mutations within this domain using a rapid and sensitive pcr-based assay for gemin ... | 1995 | 7645202 |
| serological relationships and epitope profiles of isolates of okra leaf curl geminivirus from africa and the middle east. | a panel of seven murine monoclonal antibodies (mabs) was raised against particles of okra leaf curl virus (olcv), a whitefly-transmitted geminivirus which is prevalent in west africa. the mabs detected at least six distinguishable epitopes, two continuous and four discontinuous. in tests with these mabs, relatively little antigenic variation was found among 24 olcv isolates from burkina faso, chad, ghana, ivory coast, nigeria, oman and saudi arabia, each virus isolate reacting with at least five ... | 1993 | 7506936 |
| potential gene products of bean golden mosaic virus have higher sequence homologies to those of tomato golden mosaic virus than to those of cassava latent virus. | comparison of the nucleotide sequences of the dnas of bean golden mosaic virus (bgmv), tomato golden mosaic virus (tgmv) and cassava latent virus (clv) revealed a fairly close relationship between bgmv dna1, tgmv dna1, and clv dna1 and a comparatively distant relationship between bgmv dna2, tgmv dna2, and clv dna2. the 200-base region common to the two dnas of each virus had little sequence homology, except for a highly conserved 33-36 base sequence potentially capable of forming a stable hairpi ... | 1988 | 3238924 |
| computer analysis between nucleotide and amino acid sequences of bean golden mosaic virus and those of maize streak, wheat dwarf, chloris striate mosaic, and beet curly top viruses. | bean golden mosaic virus (bgmv) dna 1 and 2 have little sequence homology with maize streak virus (msv), wheat dwarf virus (wdv), and chloris striate mosaic virus (csmv) dnas. bgmv dna 1 and beet curly top virus (bctv) dna are closely related, whereas bgmv dna 2 and bctv dna are not related. direct amino acid homologies of predicted proteins between bgmv orfs and msv orfs, wdv orfs or csmv orfs were 40-50%. bgmv 1l1 and bctv l1, and bgmv il3 and bctv l4 were highly conserved. the sequence taatat ... | 1989 | 2615677 |
| use of the asymmetric polymerase chain reaction and dna sequencing to determine genetic variability of bean golden mosaic geminivirus in the dominican republic. | a combination of the polymerase chain reaction (pcr), asymmetric pcr (a-pcr) and dna sequencing was used to determine the nucleotide sequence of a hypervariable region of the bipartite genome of bean golden mosaic geminivirus (bgmv). this region, which was part of the intergenic region of the dna-b component, was amplified using primers designed from the nucleotide sequence of a dna-b component clone (pdrb1) of an isolate of bgmv from the dominican republic (bgmv-dr). pdrb1 is infectious on bean ... | 1991 | 1940873 |
| identification of the coat protein gene of bean golden mosaic geminivirus. | an open-reading frame designated 1r1, on dna component 1 of bean golden mosaic geminivirus (bgmv), has been identified as the coat protein gene. a drai restriction fragment of bgmv dna1 that includes 1r1 was inserted into the smai pkk223-3 expression vector. the 32 kd protein expressed in escherichia coli cells reacted with antibodies to the bgmv capsid polypeptide and behaved identically to purified capsid protein in western blots. | 1991 | 1864720 |
| variability in geminivirus isolates associated with phaseolus spp. in brazil. | abstract bean golden mosaic geminivirus (bgmv) is the single most devastating virus of common beans in the tropical and subtropical americas and the caribbean basin. the bgmv from brazil, named bgmv-bz, is considered distinct from bgmv-pr isolates from puerto rico, guatemala, and the dominican republic because of dna sequence data, the ability to form pseudorecombinants, and mechanical transmissibility properties. in bean-growing areas of brazil, samples were collected from beans, lima beans, an ... | 1999 | 18944768 |
| trans-dominant inhibition of geminiviral dna replication by bean golden mosaic geminivirus rep gene mutants. | abstract geminiviruses are a group of single-stranded dna viruses that cause major losses on a number of important crops throughout the world. bean golden mosaic virus (bgmv) is a typical bipartite, whitefly-transmitted geminivirus that causes a severe disease on beans (phaseolus vulgaris) in the western hemisphere. the lack of natural resistance to geminiviruses has led to attempts to engineer resistance, particularly through the use of pathogen-derived resistance strategies. the rep gene conta ... | 1999 | 18944720 |
| biological and molecular properties of a begomovirus from dicliptera sexangularis. | abstract sixangle foldwing, dicliptera sexangularis (acanthaceae), showing severe yellow mottle and leaf distortion symptoms was collected from the shoreline of calusa island (lee county, fl). the putative virus was transmitted from infected d. sexangularis to healthy seedlings by mechanical, whitefly (bemisia tabaci biotype b), and graft-inoculations. different forms of geminivirus-like dnas were detected in total dna extracted from infected plants by southern blot hybridization analyses using ... | 2000 | 18944491 |
| the composition of bean golden mosaic virus and its single-stranded dna genome. | bean golden mosaic virus (bgmv) contains predominantly circular single-stranded dna, but dna isolation by treating virus with proteinase k results in conversion of the dna to a linear form. the conversion is inhibited by as little as 0.025% sodium dodecyl sulfate but not by phenylmethylsulfonyl fluoride and does not occur when purified dna is incubated with proteinase k. protein is not involved in the covalent structure of bgmv dna; the conversion appears to be caused by an endonuclease that cop ... | 1980 | 18631717 |
| the size and topology of single-stranded dna from bean golden mosaic virus. | the single-stranded dna (ssdna) of bean golden mosaic virus (bgmv) was resolved into two components by polyacrylamide gel (2.5%) electrophoresis in 8 m urea and by rate-zonal sedimentation in 5-20% alkaline sucrose density gradients. viral dna, either unfractionated or fractionated and electroeluted from polyacrylamide gels, was spread for electron microscopic examination. unfractionated dna and the slower of the two fractionated components contained predominantly circular molecules. the faster ... | 1979 | 18631605 |
| [biological aspects of bemisia tabaci (genn.) b biotype (hemiptera: aleyrodidae) on six bean genotypes]. | the silverleaf whitefly is one of the most harmful pests that attack bean crops, mainly for extracting large quantities of phloem sap and transmitting the bean golden mosaic virus. resistant germoplasm plants can be an important method for controlling this pest. the biological aspects of bemisia tabaci b biotype on bean (phaseolus vulgaris) genotypes were evaluated. the tests were conducted under laboratory conditions, with the following genotypes: arc 1, arc 3s, arc 5s, g13028, g11056 and porri ... | 2008 | 18506300 |
| rnai-mediated resistance to bean golden mosaic virus in genetically engineered common bean (phaseolus vulgaris). | bean golden mosaic virus (bgmv) is transmitted by the whitefly bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (phaseolus vulgaris l.). the characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. the disease is the largest constraint to bean production in latin america and causes severe yield losses (40 to 100%). here, we explored the concept of using an rna interference construct to silence the sequence region of ... | 2007 | 17555279 |
| nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. | we have sequenced the genome of bean golden mosaic virus, which comprises two circular single-stranded dna molecules (2646 and 2587 nucleotides long) of mostly unique sequence. comparison of the sequences of bean golden mosaic virus and of cassava latent virus, which share serological relationship but are very different in host range and geographical origin, shows that each virus has a unique 200-nucleotide sequence (common region) on each 2.6-kilobase molecule of its genome. the common regions ... | 1985 | 16593562 |
| isolation and characterization of virus-specific double-stranded dna from tissues infected by bean golden mosaic virus. | a double-stranded (ds) dna which may be a replication intermediate was isolated from bean (phaseolus vulgaris l. "top crop") leaves systemically infected with bean golden mosaic virus, a whitefly-transmitted plant virus with a genome of circular single-stranded (ss) dna. the isolation method used phenol/chloroform extraction, hydroxyapatite column chromatography, and rate-zonal centrifugation. the dsdna had sequences complementary to those of viral dna. the guanine-plus-cytosine content was 35%, ... | 1981 | 16593050 |
| transgene elimination in genetically modified dry bean and soybean lines. | transgene elimination is a poorly studied phenomenon in plants. we made genetic and molecular studies of a transgenic dry bean line immune to bean golden mosaic geminivirus and a soybean line. in both lines, the transgenes were stable during the vegetative phase but were eliminated during meiosis. due to its potential biotechnological value, this transgenic line was micropropagated by grafting and the vegetative copies were studied for more than two years. more than 300 plants of progeny were ob ... | 2005 | 16110439 |
| phaseolus immature embryo rescue technology. | predominant among the production constraints of the common bean phaseolus vulgaris are infestation of ascochyta blight, bean golden mosaic virus (bgmv), and bean fly. interbreeding with phaseolus -coccineus l. and/or phaseolus polyanthus greenm has been shown to provide p. vulgaris with greater resistance to these diseases. for interspecific crosses to be successful, it is important to use p. coccineus and p. polyanthus as female parents; this prevents rapid reversal to the recurrent parent p. v ... | 2011 | 21207266 |
| comparative analysis of nutritional compositions of transgenic rnai-mediated virus-resistant bean (event emb-pv051-1) with its non-transgenic counterpart. | golden mosaic is among the most economically important diseases that severely reduce bean production in latin america. in 2011, a transgenic bean event named embrapa 5.1 (emb-pv051-1), resistant to bean golden mosaic virus, was approved for commercial release in brazil. the aim of this study was to measure and evaluate the nutritional components of the beans, as well as the anti-nutrient levels in the primary transgenic line and its derived near-isogenic lines after crosses and backcrosses with ... | 2015 | 25894661 |
| development of an event-specific hydrolysis probe quantitative real-time polymerase chain reaction assay for embrapa 5.1 genetically modified common bean (phaseolus vulgaris). | a genetically modified (gm) common bean event, namely embrapa 5.1, resistant to the bean golden mosaic virus (bgmv), was approved for commercialization in brazil. brazilian regulation for genetically modified organism (gmo) labeling requires that any food containing more than 1% gmo be labeled. the event-specific polymerase chain reaction (pcr) method has been the primary trend for gmo identification and quantitation because of its high specificity based on the flanking sequence. this work repor ... | 2014 | 25437743 |
| screening metagenomic data for viruses using the e-probe diagnostic nucleic acid assay. | next generation sequencing (ngs) is not used commonly in diagnostics, in part due to the large amount of time and computational power needed to identify the taxonomic origin of each sequence in a ngs data set. by using the unassembled ngs data sets as the target for searches, pathogen-specific sequences, termed e-probes, could be used as queries to enable detection of specific viruses or organisms in plant sample metagenomes. this method, designated e-probe diagnostic nucleic acid assay, first t ... | 2014 | 25207481 |
| contrasting genetic structure between two begomoviruses infecting the same leguminous hosts. | begomoviruses are whitefly-transmitted, ssdna plant viruses and are among the most damaging pathogens causing epidemics in economically important crops worldwide. wild/non-cultivated plants play a crucial epidemiological role, acting as begomovirus reservoirs and as 'mixing vessels' where recombination can occur. previous work suggests a higher degree of genetic variability in begomovirus populations from non-cultivated hosts compared with cultivated hosts. to assess this supposed host effect on ... | 2014 | 25028472 |
| molecular characterization of the first commercial transgenic common bean immune to the bean golden mosaic virus. | golden mosaic of common bean is caused by the bean golden mosaic virus (bgmv). the disease is one of the greatest constraints on bean production in latin america and causes significant yield losses. the rnai concept was explored to silence the rep (ac1) viral gene and a transgenic bean line immune to bgmv upon inoculation at high pressure was previously generated. identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the rnai casset ... | 2013 | 23639387 |
| primers and probes development for specific pcr detection of genetically modified common bean (phaseolus vulgaris) embrapa 5.1. | the genetically modified common bean embrapa 5.1, developed by brazilian agricultural research corporation (embrapa), is the first commercial gm plant produced in latin america. it presents high resistance to the bean golden mosaic virus. in this work, primers and probes targeting a taxon-specific reference dna sequence for the common bean (phaseolus vulgaris l.) and a construct-specific dna sequence of embrapa 5.1 gm common bean were successfully developed. the primers and probes showed high sp ... | 2012 | 22506690 |
| inheritance of foreign genes in transgenic bean (phaseolus vulgaris l.) co-transformed via particle bombardment. | exploiting the biolistic process we have generated stable transgenic bean (phaseolus vulgaris l.) plants with unlinked and linked foreign genes. co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2s albumin gene isolated from the brazil nut and the antisense sequence of ac1, ac2, ac3 and bc1 genes from the bean golden mosaic geminivirus. the results revealed a co-transformation frequency rang ... | 1996 | 24162211 |
| stable transformation of phaseolus vulgaris via electric-discharge mediated particle acceleration. | transgenic phaseolus vulgaris or common bean has been produced using electric-discharge particle acceleration. the method uses particle acceleration to introduce dna into bean seed meristems. multiple shoots are then generated and screened to recover transgenic plants at a rate of 0.03% germline transformed plants/shoot. we have been able to recover transgenic plants using both gus and herbicide screening to introduce the gus, bar, and bean golden mosaic virus coat protein genes into the navy be ... | 1993 | 24196855 |
| in situ detection of early replication phases of a gemini virus in legume protoplasts. | protoplasts of phaseolus vulgaris l. (top crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. an iodine-125 labeled probe was made from the replicative form of the virus. the localization and quantitation was done by autoradiography. cell wall removal lowered the background and allowed a more accurate analysis. rnase was used to eliminate the possibility of hybrids to rna. the evidence suggests a sequence of virus movements startin ... | 1987 | 24248933 |
| complete genome sequence of a new bipartite begomovirus infecting macroptilium lathyroides in brazil. | a distinct bipartite begomovirus was isolated in northeastern brazil infecting macroptilium lathyroides showing symptoms of yellow mosaic. the complete genome (dna-a and dna-b) of the virus was cloned using rolling circle amplification and subsequently sequenced. clones presented the typical genomic organization of a new world bipartite begomovirus. based on the current taxonomic criteria established for the genus begomovirus, the virus corresponds to a new species, showing highest nucleotide id ... | 2017 | 28779234 |