| use of the asymmetric polymerase chain reaction and dna sequencing to determine genetic variability of bean golden mosaic geminivirus in the dominican republic. | a combination of the polymerase chain reaction (pcr), asymmetric pcr (a-pcr) and dna sequencing was used to determine the nucleotide sequence of a hypervariable region of the bipartite genome of bean golden mosaic geminivirus (bgmv). this region, which was part of the intergenic region of the dna-b component, was amplified using primers designed from the nucleotide sequence of a dna-b component clone (pdrb1) of an isolate of bgmv from the dominican republic (bgmv-dr). pdrb1 is infectious on bean ... | 1991 | 1940873 |
| pseudorecombination between infectious cloned dna components of tomato mottle and bean dwarf mosaic geminiviruses. | a newly described whitefly-transmitted geminivirus infecting tomato plants in florida induces yellow mottling symptoms on leaves, and stunted and distorted growth. the dna-a and dna-b components were cloned from extracts of field-infected tomato tissue; excised monomers or uncut tandem dimers of these clones were infectious when co-inoculated on to nicotiana benthamiana by rub-inoculation. tomato plants inoculated directly with the dna-a and dna-b dimers, or indirectly by sap or graft transmissi ... | 1993 | 8423448 |
| increased pathogenicity in a pseudorecombinant bipartite geminivirus correlates with intermolecular recombination. | most whitefly-transmitted geminiviruses possess bipartite dna genomes, and this feature may facilitate viral evolution through pseudorecombination and/or recombination. to test this hypothesis, the dna-a and dna-b components of the geminiviruses bean dwarf mosaic virus (bdmv) and tomato mottle virus (tomov) were exchanged, and the resultant pseudorecombinants were serially passaged through plants. both pseudorecombinants were infectious in nicotiana benthamiana but induced attenuated symptoms an ... | 1996 | 8764054 |
| nucleotide sequence of a new bipartite geminivirus isolated from the common weed sida rhombifolia in costa rica. | the nucleotide sequence of infectious clones of a geminivirus from costa rica that infects sida rhombifolia was determined. sida golden mosaic virus (sigmv-co) has a bipartite genome (dnas a and b). computer analysis showed that the bipartite genome of sigmv-co resembles that of other whitefly-transmitted geminiviruses. the dna a (2605 nt) and dna b (2587 nt) components have little sequence homology other than within the common region (cr). analysis of dnas a and b showed that sigmv-co is closel ... | 1997 | 9225056 |
| transgenic plants expressing geminivirus movement proteins: abnormal phenotypes and delayed infection by tomato mottle virus in transgenic tomatoes expressing the bean dwarf mosaic virus bv1 or bc1 proteins. | transgenic tomato plants expressing wild-type or mutated bv1 or bc1 movement proteins from bean dwarf mosaic virus (bdmv) were generated and examined for phenotypic effects and resistance to tomato mottle virus (tomov). fewer transgenic plants were recovered with the wild-type or mutated bc1 genes, compared with the wild-type or mutated bv1 genes. transgenic tomato plants expressing the wild-type or mutated bv1 proteins appeared normal. interestingly, although bdmv induces only a symptomless inf ... | 2000 | 10707355 |
| tomato mottle taino virus pseudorecombines with pymv but not with tomov: implications for the delimitation of cis- and trans-acting replication specificity determinants. | over the last decade, the tomato production in cuba has been affected by new whitefly-associated diseases. in addition to the well-documented presence of tomato yellow leaf curl virus (tylcv) along the island, the occurrence of bipartite begomoviruses has also been reported. one of them, tentatively named tomato mottle taino virus (tomotv), has now been cloned and characterized at the molecular level. its genomic organization is similar to other bipartite geminiviruses. phylogenetic analyses pla ... | 2003 | 14505083 |
| the dna-b of the non-phloem-limited bean dwarf mosaic virus (bdmv) is able to move the phloem-limited abutilon mosaic virus (abmv) out of the phloem, but dna-b of abmv is unable to confine bdmv to the phloem. | abutilon mosaic virus (abmv) and bean dwarf mosaic virus (bdmv) are two phylogenetically related bipartite begomoviruses. while abmv is restricted to phloem, bdmv spreads to non-phloem tissues. cell-to-cell and long-distance movement of abmv and bdmv were investigated after replacing the coat protein (cp) gene with the reporter gene encoding the green fluorescence protein (gfp). the dna-a and dna-b genomic components of abmv and bdmv, and their pseudorecombinants (pr), were delivered to bean (ph ... | 2003 | 15082926 |
| interaction of dna with the movement proteins of geminiviruses revisited. | geminiviruses manage the transport of their dna within plants with the help of three proteins, the coat protein (cp), the nuclear shuttle protein (nsp), and the movement protein (mp). the dna-binding capabilities of cp, nsp, and mp of abutilon mosaic virus (abmv; family geminiviridae; genus begomovirus) were scrutinized using gel mobility shift assays and electron microscopy. cp and nsp revealed a sequence-independent affinity for both double-stranded and single-stranded dna, as has been previou ... | 2004 | 15220444 |
| a viral resistance gene from common bean functions across plant families and is up-regulated in a non-virus-specific manner. | genes involved in a viral resistance response in common bean (phaseolus vulgaris cv. othello) were identified by inoculating a geminivirus reporter (bean dwarf mosaic virus expressing the green fluorescent protein), extracting rna from tissue undergoing the defense response, and amplifying sequences with degenerate r gene primers. one such gene (a tir-nbs-lrr gene, rt4-4) was selected for functional analysis in which transgenic nicotiana benthamiana were generated and screened for resistance to ... | 2006 | 16880399 |
| permeabilized mammalian cells as an experimental system for nuclear import of geminiviral karyophilic proteins and of synthetic peptides derived from their nuclear localization signal regions. | the plant-infecting geminiviruses deliver their genome and viral proteins into the host cell nucleus. members of the family geminiviridae possess either a bipartite genome composed of two approximately 2.6 kb dnas or a monopartite genome of approximately 3.0 kb dna. the bipartite genome of bean dwarf mosaic virus (bdmv) encodes several karyophilic proteins, among them the capsid protein (cp) and bv1 (nuclear shuttle protein). a cp is also encoded by the monopartite genome of tomato yellow leaf c ... | 2006 | 16894212 |
| phosphorylation of movement proteins by the plasmodesmal-associated protein kinase. | plant viruses encode movement proteins (mps) which play important roles in spreading their infectious materials throughout host plants. this infection is facilitated by cell-to-cell trafficking of mps through specialized channels termed plasmodesmata, which involves specific interactions between mps and host factors. recently, we have reported the identification of a host protein kinase named plasmodesmal-associated protein kinase (papk) which specifically phosphorylates a subset of noncell auto ... | 2008 | 18370285 |
| tomato yellow leaf curl virus infection of a resistant tomato line with a silenced sucrose transporter gene leht1 results in inhibition of growth, enhanced virus spread, and necrosis. | to identify genes involved in resistance of tomato to tomato yellow leaf curl virus (tylcv), cdna libraries from lines resistant (r) and susceptible (s) to the virus were compared. the hexose transporter leht1 was found to be expressed preferentially in r tomato plants. the role of leht1 in the establishment of tylcv resistance was studied in r plants where leht1 has been silenced using tobacco rattle virus-induced gene silencing (trv vigs). following tylcv inoculation, leht1-silenced r plants s ... | 2010 | 19946703 |
| characterization of a novel toll/interleukin-1 receptor (tir)-tir gene differentially expressed in common bean (phaseolus vulgaris cv. othello) undergoing a defence response to the geminivirus bean dwarf mosaic virus. | summary common bean (phaseolus vulgaris l.) cultivar (cv.) othello develops a hypersensitive response-associated vascular resistance to infection by bean dwarf mosaic virus (bdmv), a single-stranded dna virus (genus begomovirus, family geminiviridae). a pcr-based cdna subtraction approach was used to identify genes involved in this resistance response. eighteen clones, potentially involved with bdmv resistance, were identified based upon being up-regulated in bdmv-infected tissues and/or having ... | 2007 | 20507487 |
| tomato chlorotic leaf distortion virus, a new bipartite begomovirus infecting solanum lycopersicum and capsicum chinense in venezuela. | virus isolate t217l was obtained from a diseased tomato (solanum lycopersicum) plant showing leaf deformation and chlorotic mottle symptoms near maracaibo in the state of zulia, venezuela. full-length dna-a and dna-b molecules of t217l were cloned and sequenced. the genome organization of t217l was identical to the bipartite genomes of other begomoviruses described from the americas. characteristic disease symptoms were reproduced in s. lycopersicum and capsicum annum plants inoculated using the ... | 2011 | 21853328 |
| blocking single-stranded transferred dna conversion to double-stranded intermediates by overexpression of yeast dna replication factor a. | agrobacterium tumefaciens delivers its single-stranded transferred dna (t-strand) into the host cell nucleus, where it can be converted into double-stranded molecules. various studies have revealed that double-stranded transfer dna (t-dna) intermediates can serve as substrates by as yet uncharacterized integration machinery. nevertheless, the possibility that t-strands are themselves substrates for integration cannot be ruled out. we attempted to block the conversion of t-strands into double-str ... | 2015 | 25424309 |
| two proteins of a plant dna virus coordinate nuclear and plasmodesmal transport. | plant viruses establish a systemic infection by moving through plasmodesmata, but little is known of the mechanism(s) involved. the roles of two movement-associated proteins of a single-stranded dna virus were investigated in vivo, using functional proteins expressed in e. coli and microinjection into plant cells. we report here that the bl1 protein of bean dwarf mosaic geminivirus moves extensively from cell to cell, increases mesophyll plasmodesmal size exclusion limit, and potentiates the mov ... | 1994 | 8124726 |
| bean dwarf mosaic virus: a model system for the study of viral movement. | bean dwarf mosaic virus-[colombia:1987] (bdmv-[co:87]) is a single-stranded plant dna virus, a member of the genus begomovirus of the family geminiviridae. | 2010 | 20618704 |
| molecular identification of a new begomovirus infecting yellow passion fruit (passiflora edulis) in colombia. | the complete genome sequence of a bipartite begomovirus (genus begomovirus, family geminiviridae) infecting yellow passion fruit (passiflora edulis) in the state of valle del cauca (colombia) has been determined. the complete dna-a and dna-b components were determined to be 2600 and 2572 nt in length, respectively. the dna-a showed the highest nucleotide sequence identity (87.2 %) to bean dwarf mosaic virus (m88179), a begomovirus found in common bean crops in colombia, and only 77.4 % identity ... | 2017 | 27743253 |
| histone h3 interacts and colocalizes with the nuclear shuttle protein and the movement protein of a geminivirus. | geminiviruses are plant-infecting viruses with small circular single-stranded dna genomes. these viruses utilize nuclear shuttle proteins (nsps) and movement proteins (mps) for trafficking of infectious dna through the nuclear pore complex and plasmodesmata, respectively. here, a biochemical approach was used to identify host factors interacting with the nsp and mp of the geminivirus bean dwarf mosaic virus (bdmv). based on these studies, we identified and characterized a host nucleoprotein, his ... | 2011 | 21900168 |
| the n-terminus of the begomovirus nuclear shuttle protein (bv1) determines virulence or avirulence in phaseolus vulgaris. | the bv1 gene of the bipartite begomovirus genome encodes a nuclear shuttle protein (nsp) that is also an avirulence determinant in common bean. the function of the nsp of two common bean-infecting bipartite begomoviruses, bean dwarf mosaic virus (bdmv) and bean golden yellow mosaic virus (bgymv), was investigated using a series of hybrid dna-b components expressing chimeric bdmv and bgymv nsp, and genotypes of the two major common bean gene pools: andean (cv. topcrop) and middle american (cvs. a ... | 2007 | 17990960 |
| genetics of resistance to the geminivirus, bean dwarf mosaic virus, and the role of the hypersensitive response in common bean. | bean dwarf mosaic virus (bdmv) is a single-stranded dna virus (genus: begomovirus, family: geminiviridae) that infects common bean ( phaseolus vulgaris l.) and causes stunted plant growth, and mosaic and mottle symptoms in leaves. bdmv shows differential pathogenicity in common bean, infecting germplasm of the andean gene pool (e.g., the snap bean cultivar topcrop), but not that of the middle american gene pool (e.g., the pinto bean cultivar othello). resistance to bdmv in othello is associated ... | 2004 | 14625673 |
| bean dwarf mosaic virus bv1 protein is a determinant of the hypersensitive response and avirulence in phaseolus vulgaris. | the capacities of the begomoviruses bean dwarf mosaic virus (bdmv) and bean golden yellow mosaic virus (bgymv) to differebean dwarf mosaic viru certain common bean (phaseolus vulgaris) cultivars were used to identify viral determinants of the hypersensitive response (hr) and avirulence (avr) in bdmv. a series of hybrid dna-b components, containing bdmv and bgymv sequences, was constructed and coinoculated with bdmv dna-a (bdmv-a) or bdmva-green florescent protein into seedlings of cv. topcrop (s ... | 2000 | 11059485 |
| bean dwarf mosaic geminivirus movement proteins recognize dna in a form- and size-specific manner. | plant viral movement proteins mediate the cell-to-cell movement of nucleic acids. this involves either a direct interaction between the viral movement protein and the nucleic acid or an indirect interaction involving host factors. the bipartite geminiviruses possess two movement proteins, bv1 and bc1, that coordinate movement of viral dna across nuclear and plasmodesmal boundaries, respectively. here, we demonstrate that both bv1 and bc1 interact directly with dna and, in addition, that they hav ... | 1998 | 9778251 |