polymerase chain reaction detection of viruliferous bemisia tabaci (homoptera: aleyrodidae) with two tomato-infecting geminiviruses.the sweetpotato whitefly, bemisia tabaci (gennadius), is an important pest worldwide. a new biotype of sweetpotato whitefly, biotype b, causes damage by direct feeding and by the transmission of plant viruses, such as geminiviruses. in the mediterranean area, tomato yellow leaf curl geminivirus (tylcv) is the most serious disease of tomatoes. another whitefly-transmitted geminivirus, tomato mottle geminivirus (tomov), is presently a serious problem in tomato production in west-central and southw ...19947962950
havana tomato virus, a new bipartite geminivirus infecting tomatoes in cuba.the cloning and nucleotide sequence of a new bipartite geminivirus found in cuba is described. dna a (2620 nt) and dna b (2586 nt) presented a genomic structure resembling that of other geminiviruses transmitted by bemisia tabaci. both components had a common region of 168 nt with a 95% identity. typical elements involved in replication and transcription were found in this region, though group-characteristic arrangement of iterons was not conserved in this virus. sequence was compared with gemin ...19989787659
effect of geminivirus infection and bemisia infestation on accumulation of pathogenesis-related proteins in tomato.the whitefly, bemisia tabaci biotype b, has been shown to cause pathogenesis-related (pr) proteins to accumulate in plants as a result of direct feeding, but their specific role in plant defensive systems is unclear. our objective was to compare accumulation of tomato pr proteins (beta-1,3-glucanase, chitinase, peroxidase, p2 and p4) in response to whitefly, with or without tomato mottle virus (tomov) infection. tomato pr protein response was measured over time in plants divided into three treat ...200211921078
differential transcriptional activity of plant-pathogenic begomoviruses in their whitefly vector (bemisia tabaci, gennadius: hemiptera aleyrodidae).plant-pathogenic begomoviruses have a complex association with their whitefly vector and aspects concerning virus genetic activity (genome replication and gene transcription) within the insect remain highly controversial. virus transcript abundance was assessed by quantifying selected gene transcripts of tomato mottle virus (tomov, a new world bipartite begomovirus) and tomato yellow leaf curl virus (tylcv, an old world monopartite begomovirus) in whiteflies (bemisia tabaci biotype b) after feed ...200515831966
whitefly (bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cdna libraries.the past three decades have witnessed a dramatic increase in interest in the whitefly bemisia tabaci, owing to its nature as a taxonomically cryptic species, the damage it causes to a large number of herbaceous plants because of its specialized feeding in the phloem, and to its ability to serve as a vector of plant viruses. among the most important plant viruses to be transmitted by b. tabaci are those in the genus begomovirus (family, geminiviridae). surprisingly, little is known about the geno ...200616608516
fine mapping of the tomato yellow leaf curl virus resistance gene ty-2 on chromosome 11 of tomato.resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (tylcv), have been introgressed to cultivated tomato (solanum lycopersicum) from wild tomato accessions. a major gene, ty-2 from s. habrochaites f. glabratum accession "b6013," that confers resistance to tylcv was previously mapped to a 19-cm region on the long arm of chromosome 11. in the present study, approximately 11,000 plants were screened and nearly 157 recombination events ...201425076841
evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics.plant viruses in the genus begomovirus, family geminiviridae often cause substantial crop losses. these viruses have been emerging in many locations throughout the tropics and subtropics. like many plant viruses, they are often not recognized by plant diagnostic clinics due in large part to the lack of rapid and cost effective assays. an isothermal amplification assay, recombinase polymerase amplification (rpa), was evaluated for its ability to detect three begomoviruses and for its suitability ...201627000806
snrk1 phosphorylation of al2 delays cabbage leaf curl virus infection in arabidopsis.geminivirus al2/c2 proteins play key roles in establishing infection and causing disease in their plant hosts. they are involved in viral gene expression, counter host defenses by suppressing transcriptional gene silencing, and interfere with the host signaling involved in pathogen resistance. we report here that begomovirus and curtovirus al2/c2 proteins interact strongly with host geminivirus rep-interacting kinases (griks), which are upstream activating kinases of the protein kinase snrk1, a ...201424990996
psa causes oncogenic suppression of agrobacterium by inhibiting vire2 protein export.when coresident with the ti (tumor-inducing) plasmid, the 21-kda product of the osa gene of the plasmid psa can suppress crown gall tumorigenesis incited by agrobacterium tumefaciens. neither t-dna processing nor vir (virulence) gene induction is affected by the presence of osa in the bacterium. we used arabidopsis thaliana root segments and tobacco leaf discs to demonstrate that osa inhibits a. tumefaciens from transforming these plants to the stable phenotypes of tumorigenesis, kanamycin resis ...19999864329
peptide aptamers that bind to geminivirus replication proteins confer a resistance phenotype to tomato yellow leaf curl virus and tomato mottle virus infection in tomato.geminiviruses constitute a large family of single-stranded dna viruses that cause serious losses in important crops worldwide. they often exist in disease complexes and have high recombination and mutation rates, allowing them to adapt rapidly to new hosts and environments. thus, an effective resistance strategy must be general in character and able to target multiple viruses. the geminivirus replication protein (rep) is a good target for broad-based disease control because it is highly conserve ...201323824791
evaluation of a sumo e2 conjugating enzyme involved in resistance to clavibacter michiganensis subsp. michiganensis in solanum peruvianum, through a tomato mottle virus vigs assay.clavibacter michiganensis subsp. michiganensis (cmm) causes bacterial wilt and canker of tomato. currently, no solanum lycopersicum resistant varieties are commercially available, but some degree of cmm resistance has been identified in solanum peruvianum. previous research showed up-regulation of a sumo e2 conjugating enzyme (scei) transcript in s. peruvianum compared to s. lycopersicum following infection with cmm. in order to test the role of scei in resistance to cmm, a fragment of scei from ...201526734014
identification of begomoviruses infecting crops and weeds in belize.plants including pepper, red kidney bean, squash, string bean and tomato, as well as weeds with viral symptoms were collected from five districts in belize over a three year period with the aim of determining the diversity of the begomoviruses present. sixty five percent of the samples screened via dna hybridization produced signals indicative of begomovirus infection. subsequent pcr amplifications and nucleotide sequence analyses revealed the presence of four begomoviruses in belize. pepper gol ...200820596296
inoculation of plants with begomoviruses by particle bombardment without cloning: using rolling circle amplification of total dna from infected plants and whiteflies.a new system for inoculation of plants with begomoviral dna without cloning or the use insect vectors is described. total dna extracted from begomovirus-infected plants was amplified by rolling circle amplification (rca) using the bacteriophage phi29 dna polymerase, and inoculated to plants by particle bombardment. infection rates of up to 100% were obtained using this technique. this technique successfully inoculated all the begomoviruses evaluated: five bipartite (bean golden yellow mosaic vir ...201020447420
complementation of coat protein mutants of pepper huasteco geminivirus in transgenic tobacco plants.abstract the role of the pepper huasteco virus (phv) coat protein (cp) gene during the infection was investigated in three different hosts by using mutations that produced truncated proteins and by complementation assays in transgenic plants. the infectivity analysis revealed that mutants that express truncated cp (cp7 and cp191) behave like the wild-type virus when inoculated onto pepper and nicotiana benthamiana plants in terms of symptom expression and viral dna movement. on the contrary, the ...199918944688
induced systemic resistance and promotion of plant growth by bacillus spp.abstract elicitation of induced systemic resistance (isr) by plant-associated bacteria was initially demonstrated using pseudomonas spp. and other gram-negative bacteria. several reviews have summarized various aspects of the large volume of literature on pseudomonas spp. as elicitors of isr. fewer published accounts of isr by bacillus spp. are available, and we review this literature for the first time. published results are summarized showing that specific strains of the species b. amyloliquef ...200418944464
preliminary identification and coat protein gene phylogenetic relationships of begomoviruses associated with native flora and cultivated plants from the yucatan peninsula of mexico.a number of native and cultivated eudicots in the yucatan peninsula of mexico (ypm) exhibit symptoms associated with virus infection. symptomatic leaves were collected and assessed for begomoviral detection using polymerase chain reaction (pcr), and universal primers that amplify a fragment of the coat protein gene (core cp). begomovirus were detected in nine native and seven cultivated species, representing seven eudicot families. dna extracts from the 16 hosts were used for pcr amplification a ...200717682933
tomato mottle taino virus pseudorecombines with pymv but not with tomov: implications for the delimitation of cis- and trans-acting replication specificity determinants.over the last decade, the tomato production in cuba has been affected by new whitefly-associated diseases. in addition to the well-documented presence of tomato yellow leaf curl virus (tylcv) along the island, the occurrence of bipartite begomoviruses has also been reported. one of them, tentatively named tomato mottle taino virus (tomotv), has now been cloned and characterized at the molecular level. its genomic organization is similar to other bipartite geminiviruses. phylogenetic analyses pla ...200314505083
transgenic plants expressing geminivirus movement proteins: abnormal phenotypes and delayed infection by tomato mottle virus in transgenic tomatoes expressing the bean dwarf mosaic virus bv1 or bc1 proteins.transgenic tomato plants expressing wild-type or mutated bv1 or bc1 movement proteins from bean dwarf mosaic virus (bdmv) were generated and examined for phenotypic effects and resistance to tomato mottle virus (tomov). fewer transgenic plants were recovered with the wild-type or mutated bc1 genes, compared with the wild-type or mutated bv1 genes. transgenic tomato plants expressing the wild-type or mutated bv1 proteins appeared normal. interestingly, although bdmv induces only a symptomless inf ...200010707355
phenotypic variation in transgenic tobacco expressing mutated geminivirus movement/pathogenicity (bc1) proteins.tobacco plants were transformed with the movement protein (pathogenicity) gene (bc1) from tomato mottle geminivirus (tmov), using agrobacterium-mediated transformation. different transgenic tobacco lines that expressed high levels of the bc1 protein had phenotypes ranging from plants with severe stunting and leaf mottling (resembling geminivirus symptoms) to plants with no visible symptoms. the sequence data for the bc1 transgene from the transgenic plants with the different phenotypes indicated ...19979390421
increased pathogenicity in a pseudorecombinant bipartite geminivirus correlates with intermolecular recombination.most whitefly-transmitted geminiviruses possess bipartite dna genomes, and this feature may facilitate viral evolution through pseudorecombination and/or recombination. to test this hypothesis, the dna-a and dna-b components of the geminiviruses bean dwarf mosaic virus (bdmv) and tomato mottle virus (tomov) were exchanged, and the resultant pseudorecombinants were serially passaged through plants. both pseudorecombinants were infectious in nicotiana benthamiana but induced attenuated symptoms an ...19968764054
pseudorecombination between infectious cloned dna components of tomato mottle and bean dwarf mosaic geminiviruses.a newly described whitefly-transmitted geminivirus infecting tomato plants in florida induces yellow mottling symptoms on leaves, and stunted and distorted growth. the dna-a and dna-b components were cloned from extracts of field-infected tomato tissue; excised monomers or uncut tandem dimers of these clones were infectious when co-inoculated on to nicotiana benthamiana by rub-inoculation. tomato plants inoculated directly with the dna-a and dna-b dimers, or indirectly by sap or graft transmissi ...19938423448
the nucleotide sequence of tomato mottle virus, a new geminivirus isolated from tomatoes in florida.a new geminivirus, tomato mottle virus (tmov), affecting tomato production in florida has been cloned and sequenced. sequence analysis of the cloned replicative forms of tmov revealed four potential coding regions for the a component [2601 nucleotides (nt)] and two for the b component (2541 nt). comparisons of the nucleotide sequence of the tmov genome with those of other whitefly-transmitted geminiviruses indicate that tmov is a typical bipartite geminivirus of the new world and is closely rela ...19921469361
multitrophic interactions of the silverleaf whitefly, host plants, competing herbivores, and phytopathogens.our laboratory found that silverleaf whitefly (slw; bemisia argentifolii bellows & perring) feeding alters host plant physiology and chemistry. the slw induces a number of host plant defenses, including pathogenesis-related (pr) protein accumulation (e.g., chitinases, beta-1,3-glucanases, peroxidases, chitosanases, etc.). induction of the pr proteins by slw feeding occurs in various plant species and varieties. the extent and type of induction is dependent on a number of factors that include hos ...200212432517
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