| molecular characterization of a new whitefly-transmissible bipartite geminivirus infecting tomato in panama. | the nucleotide sequence of infectious clones of a tomato-infecting geminivirus from panama [named tomato leaf curl virus (tolcv-pan) because of symptoms produced in infected tomato (plant stunting and mild leaf curling)] was determined. tolcv-pan has a bipartite genome (dnas a and b) and computer analysis showed that the genome resembles that of other bipartite, whitefly-transmitted geminiviruses. dna a (2584 nt) and b (2542 nt) have little sequence homology other than within the common region. ... | 1998 | 9780034 |
| expression of the oligomerization domain of the replication-associated protein (rep) of tomato leaf curl new delhi virus interferes with dna accumulation of heterologous geminiviruses. | the minimal dna binding domain of the replication-associated protein (rep) of tomato leaf curl new delhi virus was determined by electrophoretic mobility gel shift analysis and co-purification assays. dna binding activity maps to amino acids 1-160 (rep-(1-160)) of the rep protein and overlaps with the protein oligomerization domain. transient expression of rep protein (rep-(1-160)) was found to inhibit homologous viral dna accumulation by 70-86% in tobacco protoplasts and in nicotiana benthamian ... | 2001 | 11342533 |
| tomato mottle taino virus pseudorecombines with pymv but not with tomov: implications for the delimitation of cis- and trans-acting replication specificity determinants. | over the last decade, the tomato production in cuba has been affected by new whitefly-associated diseases. in addition to the well-documented presence of tomato yellow leaf curl virus (tylcv) along the island, the occurrence of bipartite begomoviruses has also been reported. one of them, tentatively named tomato mottle taino virus (tomotv), has now been cloned and characterized at the molecular level. its genomic organization is similar to other bipartite geminiviruses. phylogenetic analyses pla ... | 2003 | 14505083 |
| biological and molecular properties of a begomovirus from dicliptera sexangularis. | abstract sixangle foldwing, dicliptera sexangularis (acanthaceae), showing severe yellow mottle and leaf distortion symptoms was collected from the shoreline of calusa island (lee county, fl). the putative virus was transmitted from infected d. sexangularis to healthy seedlings by mechanical, whitefly (bemisia tabaci biotype b), and graft-inoculations. different forms of geminivirus-like dnas were detected in total dna extracted from infected plants by southern blot hybridization analyses using ... | 2000 | 18944491 |
| distribution and diversity of geminiviruses in trinidad and tobago. | abstract seven crop and eight weed species from 12 agricultural locations in trinidad and tobago were assayed for the presence of whitefly-transmitted geminiviruses (wtgs) by using dot blot hybridization and polymerase chain reaction (pcr) amplification of the n-terminal coat protein sequence with degenerate primers. the amplified fragments were cloned and analyzed by restriction enzyme digestion to determine fragment length polymorphism among the cloned fragments. representative clones were the ... | 1998 | 18944827 |
| molecular characterization and experimental host range of an isolate of macroptilium golden mosaic virus that infects wissadula amplissima in jamaica. | partial genome sequences for the tentative begomovirus macroptilium golden mosaic virus (mgmv) have been previously reported and were originally obtained for an isolate that infected macroptilium lathyroides in jamaica. in this study, we pcr-amplified, cloned and determined the sequence for the complete genome of isolates of mgmv that we found infecting wissadula amplissima collected from august town and spanish town, jamaica. sequence analysis confirmed that mgmv is a distinct begomovirus speci ... | 2010 | 20347895 |
| a novel synthetic quantification standard including virus and internal report targets: application for the detection and quantification of emerging begomoviruses on tomato. | abstract: background: begomovirus is a genus of phytopathogenic single-stranded dna viruses, transmitted by the whitefly bemisia tabaci. this genus includes emerging and economically significant viruses such as those associated with tomato yellow leaf curl disease, for which diagnostic tools are needed to prevent dispersion and new introductions. five real-time pcrs with an internal tomato reporter gene were developed for accurate detection and quantification of monopartite begomoviruses, includ ... | 2011 | 21819593 |
| characterization of potato yellow mosaic virus as a geminivirus with a bipartite genome. | a whitefly-transmitted virus from venezuela, potato yellow mosaic virus, has been propagated, isolated and characterized as a member of the geminivirus group. the virus was transmitted to several species of nicotiana and to petunia hybrida by mechanical inoculation, and to potato and tomato plants by grafting. purified virus possessed typical geminate particle morphology and encapsidated both genomic and subgenomic species consisting of single-stranded dna. the genome of the virus was cloned int ... | 2011 | 3182230 |
| characterization and genetic diversity of potato yellow mosaic virus from the caribbean. | the begomovirus potato yellow mosaic virus (pymv) is responsible of significant yield losses in tomato in guadeloupe. four field isolates from guadeloupe were analyzed in term of their host range using three inoculation methods (mechanical, grafting and insect vector), sequences analysis of pcr fragments and phylogenetic analysis of an infectious clone, pymv-[gp]. capsicum annuum, datura stramonium, nicotiana benthamiana, n. tabacum 'xanthi nc', petunia hybrida, and solanum tuberosum were found ... | 2004 | 14745605 |
| potato yellow mosaic virus: a synonym of tomato yellow mosaic virus. | tomato yellow mosaic was first described in 1963, as a disease caused by a geminivirus transmitted by the whitefly bemisia tabaci in venezuela. in 1981 and 1985, tomato yellow mosaic virus (toymv) was reported to occasionally infect potato plants growing in the proximity of tomato plantings affected by this virus. despite these previous reports, a virus isolated from yellow mosaic-affected potato plants in venezuela, was described in 1986 as a "new geminivirus" called potato yellow mosaic virus ... | 2001 | 11765926 |
| the nucleotide sequence of the infectious cloned dna components of potato yellow mosaic virus. | the complete nucleotide sequence of a venezuelan isolate of potato yellow mosaic virus (pymv) has been determined, showing it to be typical of subgroup i geminiviruses in that it is whitefly-transmitted, has a circular, bipartite ssdna genome and possesses bidirectionally orientated open reading frames (orfs). the two genomic components have little sequence similarity apart from a common region of 268 nucleotides (nt) which is almost identical. analysis of orfs revealed six potential coding regi ... | 1991 | 1856690 |