| the nucleotide sequence of tomato mottle virus, a new geminivirus isolated from tomatoes in florida. | a new geminivirus, tomato mottle virus (tmov), affecting tomato production in florida has been cloned and sequenced. sequence analysis of the cloned replicative forms of tmov revealed four potential coding regions for the a component [2601 nucleotides (nt)] and two for the b component (2541 nt). comparisons of the nucleotide sequence of the tmov genome with those of other whitefly-transmitted geminiviruses indicate that tmov is a typical bipartite geminivirus of the new world and is closely rela ... | 1992 | 1469361 |
| abutilon mosaic geminivirus double-stranded dna is packed into minichromosomes. | an intermediate form of abutilon mosaic geminivirus, a complex of viral double-stranded dna (dsdna) and protein, was characterized by two different techniques. cesium sulphate gradient centrifugation was used to show that the majority of this form comigrates with host chromatin. micrococcus nuclease digestion experiments with isolated nuclei demonstrated that the viral dsdna is organized in a manner comparable to that of host nucleosomes. results from a previous electron microscopical work (abou ... | 1992 | 1641992 |
| transcript mapping of abutilon mosaic virus, a geminivirus. | the transcripts of the dna of abutilon mosaic virus, a geminivirus with a bipartite genome (dna a and dna b), were characterized by northern blot hybridization, s1 nuclease assay, primer extension analysis, and sequencing of the 3' termini of cdna clones. it was shown that transcription is bidirectional and that the transcripts are polyadenylated. two overlapping transcripts of 1.6 and 0.7 kb were mapped to the complementary strand of dna a and two of 1.3 and 1.2 kb to the complementary strand o ... | 1991 | 1962440 |
| localization of abutilon mosaic virus (abmv) dna within leaf tissue by in situ hybridization. | abutilon mosaic virus (abmv) is a whitefly-transmitted geminivirus with a a bipartite genome. using in situ hybridization abmv dna was detected exclusively in the phloem of infected abutilon sellovianum leaves and intracellularly predominant in the nuclei. no abmv dna was found in cells from palisade and spongy parenchyma, the tissues which show the predominant cytopathological effects. a hypothesis is discussed to account for the finding that, while abmv accumulates in the phloem, symptoms are ... | 1991 | 2014637 |
| the nucleotide sequence of abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. | the complete nucleotide sequence of abutilon mosaic virus (west indian isolate, abmva) is presented. the resulting genomic structure resembles that of other geminiviruses which are transmitted by the whitefly bemisia tabaci: abmv possesses a bipartite circular genome with bidirectional orientation of the open reading frames (orf). both components have a common region of 180 bases with 99% homology while the rest of their sequence is distinct. eukaryotic regulatory transcription elements precede ... | 1990 | 2219703 |
| complementation and recombination between mutants of complementary sense genes of dna a of abutilon mosaic virus. | mutations have been inserted into the complementary sense orfs of dna a of abutilon mosaic virus (abmv). mutation in both orf ac1 and orf ac2/3 resulted in clones incapable of infecting nicotiana benthamiana following agroinoculation. mutation in orf ac1 but not in orf ac2/3 prevented dna replication in leaf discs. thus orf ac1 is essential for abmv dna replication whereas orf ac2/3 is probably involved in some aspect of the spread of the virus through the plant. coagroinoculation of both mutant ... | 1993 | 8212592 |
| specificity of bipartite geminivirus movement proteins. | pseudorecombinants produced by exchanging genome components (dnas a and b) of the geminiviruses african cassava mosaic virus (acmv) and indian cassava mosaic virus (icmv), acmv, and tomato golden mosaic virus (tgmv), and tgmv and abutilon mosaic virus (abmv) are not infectious in their common host nicotiana benthamiana. in each case, dna a was unable to trans-replicate the heterogenomic dna b component in a n. benthamiana leaf disc assay. the non-viability of the pseudorecombinants has been expl ... | 1993 | 8372440 |
| pseudorecombination between infectious cloned dna components of tomato mottle and bean dwarf mosaic geminiviruses. | a newly described whitefly-transmitted geminivirus infecting tomato plants in florida induces yellow mottling symptoms on leaves, and stunted and distorted growth. the dna-a and dna-b components were cloned from extracts of field-infected tomato tissue; excised monomers or uncut tandem dimers of these clones were infectious when co-inoculated on to nicotiana benthamiana by rub-inoculation. tomato plants inoculated directly with the dna-a and dna-b dimers, or indirectly by sap or graft transmissi ... | 1993 | 8423448 |
| dna b facilitates, but is not essential for, the spread of abutilon mosaic virus in agroinoculated nicotiana benthamiana. | full-length dimers of both dna a and dna b of abutilon mosaic virus have been constructed. these constructs are not infectious when inoculated into nicotiana benthamiana as dna; however, an infection could be obtained using the method of agroinoculation. symptom induction required both dna molecules but agroinoculation with dna a alone resulted in virus spread in the majority of plants. mutations were made in both of the open reading frames of dna b. coagroinoculation of wild-type dna a and muta ... | 1993 | 8503185 |
| coat protein gene replacement results in whitefly transmission of an insect nontransmissible geminivirus isolate. | geminiviruses are transmitted by whiteflies, leafhoppers, or treehoppers. the whitefly species bemisia tabaci (gennadius) is the most efficient vector of subgroup iii geminiviruses. an isolate of abutilon mosaic virus (abmv), a bipartite geminivirus, was not detectable in the dna extract from insects by southern blot analysis, nor was the isolate transmissible by the b-biotype of b. tabaci, although the virus dna was amplified (by pcr) from some insects. in contrast, sida golden mosaic virus (si ... | 1997 | 9325236 |
| host range and symptom variation of pseudorecombinant virus produced by two distinct bipartite geminiviruses. | within the whitefly group only the species bemisia tabaci (gennadius) is the vector. most whitefly-transmitted geminiviruses possess bipartite dna genomes, dnas a and b. although they are closely related to each other, the production of viable pseudorecombinants between bipartite geminiviruses by reassortment of infectious cloned components is generally limited to strains of a particular virus. following exchange of cloned genomic components of sida golden mosaic virus (sigmv/hoyv) and abutilon ... | 2000 | 10963348 |
| the groel protein of the whitefly bemisia tabaci interacts with the coat protein of transmissible and nontransmissible begomoviruses in the yeast two-hybrid system. | we have previously suggested that a groel homolog produced by the whitefly bemisia tabaci endosymbiotic bacteria interacts in the insect hemolymph with particles of tomato yellow leaf curl virus from israel (tylcv-is), ensuring the safe circulative transmission of the virus. we have now addressed the question of whether the nontransmissibility of abutilon mosaic virus from israel (abmv-is) is related to a lack of association between groel and the virus coat protein (cp). translocation analysis h ... | 2000 | 11040131 |
| fulfilling koch's postulates for abutilon mosaic virus. | cloned abutilon mosaic geminivirus (abmv) has been re-transmitted to abutilon sellovianum (syn. striatum) plants by a two-step combined agroinfection/grafting method. the symptoms induced were indistinguishable from the characteristic mosaic of ornamental abutilon plants. therefore, we can exclude that a mixture of different abmv variants is responsible for the striking variety of abutilon leaf pattern elements. analysis of the symptoms on consecutively infected leaves suggests that mosaic forma ... | 2000 | 11087104 |
| dna forms indicate rolling circle and recombination-dependent replication of abutilon mosaic virus. | geminiviruses have spread worldwide and have become increasingly important in crop plants during recent decades. recombination among geminiviruses was one major source of new variants. geminiviruses replicate via rolling circles, confirmed here by electron microscopic visualization and two-dimensional gel analysis of abutilon mosaic virus (abmv) dna. however, only a minority of dna intermediates are consistent with this model. the majority are compatible with recombination-dependent replication ... | 2001 | 11689455 |
| localizing the movement proteins of abutilon mosaic geminivirus in yeast by subcellular fractionation and freeze-fracture immuno-labelling. | the movement proteins bc1 and bv1 of abutilon mosaic geminivirus fused to glutathion-s-transferase (gst) and flag-peptide were expressed in fission yeast (schizosaccharomyces pombe) cells to analyse the fundamental intracellular distribution of these proteins in an eukaryotic cell in the absence of plant-specific factors. most of bc1 protein sedimented rapidly after cell lysis and differential centrifugation. using freeze-fracture immuno-labelling, the protein was detected in situ predominantly ... | 2002 | 11855624 |
| exchange of three amino acids in the coat protein results in efficient whitefly transmission of a nontransmissible abutilon mosaic virus isolate. | geminiviruses are transmitted in a circulative manner by whiteflies, leafhoppers, or treehoppers. the whitefly species bemisia tabaci (genn.) is the vector for members of the genus begomovirus. the closely related bipartite central american begomoviruses abutilon mosaic virus (abmv), sida golden mosaic virus originating from costa rica (sigmv-cr), and sida golden mosaic virus originating from honduras (sigmv-hoyv) were used to study transmission by their insect vector. the abmv isolate is defect ... | 2001 | 11883001 |
| movement proteins (bc1 and bv1) of abutilon mosaic geminivirus are cotransported in and between cells of sink but not of source leaves as detected by green fluorescent protein tagging. | two movement proteins (bv1 and bc1) facilitate the intra- and intercellular transport of begomoviruses in plants. in contrast to other geminiviruses the movement protein bc1 of abutilon mosaic virus (abmv) remained in the supernatant after centrifuging plant extracts at 20,000 g. to test whether this unusual behavior results from a distinct intracellular distribution of the protein, the bc1 gene has been fused to the gene of green fluorescent protein (gfp). the resulting plasmids were delivered ... | 2001 | 11883189 |
| subcellular targeting domains of abutilon mosaic geminivirus movement protein bc1. | abutilon mosaic geminivirus (abmv) encodes two movement proteins, bv1 and bc1, which mediate the intra- and intercellular transport of viral dna in plants cooperatively. it has been shown previously that singly expressed bc1, fused to green fluorescent protein (gfp), accumulates preferentially either at the cell periphery or around the nucleus in separate plant cells. to define the bc1 domains responsible for understanding the subcellular sorting, deletion mutants were fused to gfp and expressed ... | 2002 | 12491102 |
| multitasking in replication is common among geminiviruses. | geminiviruses package single-stranded circular dna and replicate via double-stranded dna intermediates. during the past decade, increasing evidence has led to the general acceptance that their replication follows a rolling-circle replication mechanism like bacteriophages with single-stranded dna. in a recent study, we showed that this is also true for abutilon mosaic geminivirus (abmv), but that this particular virus may also use a recombination-dependent replication (rdr) route in analogy to t4 ... | 2003 | 12584322 |
| tomato mottle taino virus pseudorecombines with pymv but not with tomov: implications for the delimitation of cis- and trans-acting replication specificity determinants. | over the last decade, the tomato production in cuba has been affected by new whitefly-associated diseases. in addition to the well-documented presence of tomato yellow leaf curl virus (tylcv) along the island, the occurrence of bipartite begomoviruses has also been reported. one of them, tentatively named tomato mottle taino virus (tomotv), has now been cloned and characterized at the molecular level. its genomic organization is similar to other bipartite geminiviruses. phylogenetic analyses pla ... | 2003 | 14505083 |
| mapping of abutilon mosaic geminivirus minichromosomes. | the single-stranded circular dna of abutilon mosaic geminivirions is complemented to double-stranded dna by host proteins after infecting cells. this double-stranded dna serves as a template for replication as well as transcription and is assembled into host nucleosomes, yielding circular viral minichromosomes. their chromatin structure was analyzed by use of isolated nuclei combining nuclease sensitivity assays with ligation-mediated pcr, evaluating nucleosomal ladders and topoisomer distributi ... | 2003 | 14512531 |
| sida micrantha mosaic is associated with a complex infection of begomoviruses different from abutilon mosaic virus. | we report on the nucleotide sequences of geminiviruses of the genus bemogovirus infecting sida micrantha schr., a common weed in brazil. for decades, the mosaic frequently associated with sida plants was considered to be caused by a brazilian strain of abutilon mosaic virus (abmv). by infection studies and sequence comparisons, we demonstrate that it is associated with a complex of at least two begomoviruses as different from abmv as most south american geminiviruses. two molecules of dna a (a1, ... | 2004 | 15045569 |
| the dna-b of the non-phloem-limited bean dwarf mosaic virus (bdmv) is able to move the phloem-limited abutilon mosaic virus (abmv) out of the phloem, but dna-b of abmv is unable to confine bdmv to the phloem. | abutilon mosaic virus (abmv) and bean dwarf mosaic virus (bdmv) are two phylogenetically related bipartite begomoviruses. while abmv is restricted to phloem, bdmv spreads to non-phloem tissues. cell-to-cell and long-distance movement of abmv and bdmv were investigated after replacing the coat protein (cp) gene with the reporter gene encoding the green fluorescence protein (gfp). the dna-a and dna-b genomic components of abmv and bdmv, and their pseudorecombinants (pr), were delivered to bean (ph ... | 2003 | 15082926 |
| interaction of dna with the movement proteins of geminiviruses revisited. | geminiviruses manage the transport of their dna within plants with the help of three proteins, the coat protein (cp), the nuclear shuttle protein (nsp), and the movement protein (mp). the dna-binding capabilities of cp, nsp, and mp of abutilon mosaic virus (abmv; family geminiviridae; genus begomovirus) were scrutinized using gel mobility shift assays and electron microscopy. cp and nsp revealed a sequence-independent affinity for both double-stranded and single-stranded dna, as has been previou ... | 2004 | 15220444 |
| yeast two-hybrid systems confirm the membrane- association and oligomerization of bc1 but do not detect an interaction of the movement proteins bc1 and bv1 of abutilon mosaic geminivirus. | most of the plant begomoviruses use two proteins to transport their dna from cell to cell, bv1 to shuttle it between nucleus and cytoplasm and bc1 to facilitate movement across plasmodesmata. in order to analyse their interaction for abutilon mosaic geminivirus (abmv) in yeast ( saccharomyces cerevisiae), bc1 and bv1 genes were cloned into various plasmid vectors suitable for two-hybrid analysis. bc1 was fused to the binding domain (gbd) and bv1 to the activation domain (gad) of the gal4 transcr ... | 2004 | 15449136 |
| single-stranded dna from abutilon mosaic virus is present in the plastids of infected abutilon sellovianum. | the leaf mosaic of abutilon sellovianum is caused by abutilon mosaic virus (abmv), a geminivirus that has a circular single-stranded dna genome. dna was isolated from intact plastids of abmv-infected and noninfected plants. plastids from infected plants were shown to contain the single-stranded abmv dna by southern-blot hybridization experiments that used a probe made from highly purified abmv dna. the possibility of adsorption of abmv virions or viral dna onto the plastid envelope was ruled out ... | 1987 | 16578823 |
| synergism of a dna and an rna virus: enhanced tissue infiltration of the begomovirus abutilon mosaic virus (abmv) mediated by cucumber mosaic virus (cmv). | replication of the begomovirus abutilon mosaic virus (abmv) is restricted to phloem nuclei, generating moderate levels of virus dna. co-infection with cucumber mosaic virus (cmv) evidently increased abmv titers in nicotiana benthamiana, tobacco, and tomato, resulting in synergistic symptom enhancement. in situ hybridization revealed that in double-infected leaves an increased number of nuclei contained elevated amounts of abmv. additionally, the begomoviral phloem-limitation was broken. whereas ... | 2007 | 16959287 |
| synergistic pathogenicity of a phloem-limited begomovirus and tobamoviruses, despite negative interference. | in contrast to previous observations on phloem-limited geminiviruses supported in movement andaccumulation by rna viruses such as cucumo- and tobamoviruses, tissue infiltration by abutilon mosaic virus (abmv) was enhanced by neither tobacco mosaic virus nor tomato mosaic virus (tomv) in two different hosts, nicotiana benthamiana and tomato. both tobamoviruses exerted a negative effect on the dna virus, resulting in a decrease in abmv accumulation and significantly reduced infectivity in n. benth ... | 2007 | 17325378 |
| the movement protein bc1 promotes redirection of the nuclear shuttle protein bv1 of abutilon mosaic geminivirus to the plasma membrane in fission yeast. | in order to monitor their interaction and cellular localisation, the movement protein (mp; syn. bc1) and the nuclear shuttle protein (nsp; syn. bv1) of the geminivirus abutilon mosaic virus (abmv) were ectopically expressed in schizosaccharomyces pombe cells, either alone or together under the control of an inducible promoter. for highest resolution, electron microscopy using freeze-fracture immunolabelling served to detect these proteins in situ. as expected from previous in planta and yeast ex ... | 2007 | 17351736 |
| abutilon mosaic virus dna b component supports mechanical virus transmission, but does not counteract begomoviral phloem limitation in transgenic plants. | different nicotiana benthamiana lines stably transformed with abutilon mosaic virus (abmv) dimeric dna b were capable of systemically spreading complete bipartite abmv genomes, following agroinoculation of dna a alone. constitutively expressed viral movement protein (bc1) did not induce any persistent disease phenotype, but plants developed transient morphological abnormalities such as radially symmetric leaves after kanamycin withdrawal. systemic abmv infection produced symptoms and virus titer ... | 2007 | 17462695 |
| molecular characterization and phylogenetic relationships of two new bipartite begomovirus infecting malvaceous plants in yucatan, mexico. | sida acuta and corchorus siliquosus plants showing yellow mosaic and yellow vein symptoms, respectively, were collected in the yucatan peninsula, mexico. total dna was isolated from both plant species and used for the amplification, cloning, and sequencing of the begomovirus genome. nucleotide comparison of the complete dna-a component isolated from s. acuta and c. siliquosus confirmed the presence of two distinct begomoviruses species. based on phenotypic symptoms observed in infected field pla ... | 2007 | 17638064 |
| post-translational modifications of abutilon mosaic virus movement protein (bc1) in fission yeast. | the movement protein (mp) of abutilon mosaic virus (abmv, geminiviridae) exhibited a complex band pattern upon gel electrophoresis indicating its post-translational modification when expressed in abmv-infected plants or, ectopically, in fission yeasts. high-resolution separation according to charge and molecular weight in acetic acid/urea polyacrylamide or sodium dodecyl sulphate polyacrylamide gels followed by western blot analysis revealed a pattern of abmv mp from infected plants more related ... | 2008 | 17919761 |
| a protocol for the rapid isolation of full geminivirus genomes from dried plant tissue. | a high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an extract-n-amp-based dna isolation technique with rolling circle amplification (rca) of viral dna, is presented. using this method an attempt was made to isolate and clone full geminivirus genomes/genome components from 102 plant samples, including dried leaves stored at room temperature for between 6 months and 10 years, with an average hands-on-time to rca-ready dna of 15 min per 20 ... | 2008 | 18280590 |
| cytochemical characterization of plastidal inclusions in abutilon mosaic-infected malva parviflora mesophyll cells. | electron microscopy of plastids from mesophyll cells of malva parviflora infected with the abutilon mosaic virus revealed elongated "chains of pearls" with subunits of 7.5 nm in diameter. paracrystalline inclusions of the chains of pearls studied by means of cytochemical techniques gave evidence of the presence of dna. | 1980 | 18631715 |
| cotton leaf crumple virus is a distinct western hemisphere begomovirus species with complex evolutionary relationships indicative of recombination and reassortment. | abstract the bipartite dna genome of cotton leaf crumple virus (clcrv), a whitefly-transmitted begomovirus from the sonoran desert, was cloned and completely sequenced. the cloned clcrv genome was infectious when biolistically delivered to cotton or bean seedlings and progeny virus was whitefly-transmissible. koch's postulates were completed by the reproduction of characteristic leaf crumple symptoms in cotton seedlings infected with cloned clcrv dna, thereby verifying the etiology of leaf crump ... | 2004 | 18943795 |
| variability in geminivirus isolates associated with phaseolus spp. in brazil. | abstract bean golden mosaic geminivirus (bgmv) is the single most devastating virus of common beans in the tropical and subtropical americas and the caribbean basin. the bgmv from brazil, named bgmv-bz, is considered distinct from bgmv-pr isolates from puerto rico, guatemala, and the dominican republic because of dna sequence data, the ability to form pseudorecombinants, and mechanical transmissibility properties. in bean-growing areas of brazil, samples were collected from beans, lima beans, an ... | 1999 | 18944768 |
| three c-terminal phosphorylation sites in the abutilon mosaic virus movement protein affect symptom development and viral dna accumulation. | the abutilon mosaic virus (abmv, geminiviridae) dna b component encodes a movement protein (mp), which facilitates viral transport within plants and affects pathogenicity. the presence of phosphorylated serine and threonine residues was confirmed for mp expressed in yeast and nicotiana benthamiana by comparative western blot analysis using phospho-amino acid- and mp-specific immunodetection. mass spectrometry of yeast-derived mp identified three phosphorylation sites located in the c-terminal do ... | 2009 | 19464722 |
| expression dynamics and ultrastructural localization of epitope-tagged abutilon mosaic virus nuclear shuttle and movement proteins in nicotiana benthamiana cells. | the geminivirus abutilon mosaic virus (abmv) encodes two proteins which are essential for viral spread within plants. the nuclear shuttle protein (nsp) transfers viral dna between the nucleus and cytoplasm, whereas the movement protein (mp) facilitates transport between cells through plasmodesmata and long-distance via phloem. an inducible overexpression system for epitope-tagged nsp and mp in plants yielded unprecedented amounts of both proteins. western blots revealed extensive posttranslation ... | 2009 | 19628237 |
| molecular characterization and phylogenetic relationships of desmodium leaf distortion virus (deldv): a new begomovirus infecting desmodium glabrum in yucatan, mexico. | the complete dna-a component sequence of desmodium leaf distortion virus (deldv, begomovirus) isolated in yucatan was determined to be 2569 nucleotides (nt) in length, and it was most closely related to cotton leaf crumple virus-california (clcrv-[cal]), at 76%. the complete dna-b component sequence was 2514 nt in length, and shared its highest nucleotide identity (60%) with potato yellow mosaic trinidad virus (pymtv). phylogenetic analyses group the deldv dna-a component in the slcv clade, wher ... | 2009 | 19757008 |
| molecular characterization and experimental host range of an isolate of wissadula golden mosaic st. thomas virus. | partial genome segments of a begomovirus were previously amplified from wissadula amplissima exhibiting yellow-mosaic and leaf-curl symptoms in the parish of st. thomas, jamaica and this isolate assigned to a tentative begomovirus species, wissadula golden mosaic st. thomas virus. to clone the complete genome of this isolate of wissadula golden mosaic st. thomas virus, abutting primers were designed to pcr amplify its full-length dna-a and dna-b components. sequence analysis of the complete bego ... | 2009 | 19768650 |
| a plastid-targeted heat shock cognate 70kda protein interacts with the abutilon mosaic virus movement protein. | the movement protein (mp) of bipartite geminiviruses facilitates cell-to-cell as well as long-distance transport within plants and influences viral pathogenicity. yeast two-hybrid assays identified a chaperone, the nuclear-encoded and plastid-targeted heat shock cognate 70kda protein (cphsc70-1) of arabidopsis thaliana, as a potential binding partner for the abutilon mosaic virus (abmv) mp. in planta, bimolecular fluorescence complementation (bifc) analysis showed cphsc70-1/mp complexes and mp h ... | 2010 | 20193958 |
| the complete nucleotide sequence of a new bipartite begomovirus from brazil infecting abutilon. | the complete nucleotide sequence of abutilon mosaic brazil virus (abmbv), a new bipartite begomovirus from bahia, brazil, is described and analyzed phylogenetically. its dna a is most closely related to those of sida-infecting begomoviruses from brazil and forms a phylogenetic cluster with pepper- and euphorbia-infecting begomoviruses from central america. the dna b component forms a cluster with different sida- and okra-infecting begomoviruses from brazil. both components are distinct from thos ... | 2010 | 20349251 |
| cell-free construction of disarmed abutilon mosaic virus-based gene silencing vectors. | the bipartite abutilon mosaic virus (abmv) was engineered as a versatile silencing vector in which the coat protein gene of dna a was deleted and replaced by sequences of interest. plants transgenic for the dimeric abmv dna b component were used as test hosts to minimize the risk of unintended release of the recombinant dna. the vector construct was stable genetically upon systemic infection and, in common with the parental virus, the vector remained phloem-limited. for virus-induced gene silenc ... | 2010 | 20638413 |
| rna viruses and their silencing suppressors boost abutilon mosaic virus, but not the old world tomato yellow leaf curl sardinia virus. | mixed viral infections can induce different changes in symptom development, genome accumulation and tissue tropism. these issues were investigated for two phloem-limited begomoviruses, abutilon mosaic virus (abmv) and tomato yellow leaf curl sardinia virus (tylcsv) in nicotiana benthamiana plants doubly infected by either the potyvirus cowpea aphid-borne mosaic virus (cabmv) or the tombusvirus artichoke mottled crinkle virus (amcv). both rna viruses induced an increase of the amount of abmv, led ... | 2011 | 21843560 |
| self-interaction of abutilon mosaic virus replication initiator protein (rep) in plant cell nuclei. | geminiviruses replicate their circular single-stranded dna genome in nuclei of infected plant cells. their replication initiator proteins (reps) possess interaction domains for homo- and hetero-oligomerization as shown previously by in vitro studies and yeast two hybrid assays. here, homo-oligomerization and cellular localization of the abutilon mosaic virus (abmv) rep was analysed with bimolecular fluorescence complementation (bifc) in epidermal tissues of nicotiana benthamiana. bifc revealed t ... | 2011 | 21840354 |
| conformation-selective methylation of geminiviral dna. | geminiviruses with small circular single-stranded dna genomes replicate in plant cell nuclei using various double-stranded dna intermediates: distinct open circular and covalently closed circular as well as heterogeneous linear dna. their dna may be methylated partially at cytosine residues, which has been detected previously by bisulfite sequencing and subsequent polymerase chain reaction. in order to determine the methylation patterns of the circular molecules, the dna of tomato yellow leaf cu ... | 2011 | 21835804 |
| the induction of stromule formation by a plant dna-virus in epidermal leaf tissues suggests a novel intra- and intercellular macromolecular trafficking route. | stromules are dynamic thin protrusions of membrane envelope from plant cell plastids. despite considerable progress in understanding the importance of certain cytoskeleton elements and motor proteins for stromule maintenance, their function within the cell has yet to be unraveled. several viruses cause a remodulation of plastid structures and stromule biogenesis within their host plants. for rna-viruses these interactions were demonstrated to be relevant to the infection process. an involvement ... | 2012 | 23293643 |
| the stress granule component g3bp is a novel interaction partner for the nuclear shuttle proteins of the nanovirus pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus. | stress granules (sgs) are structures within cells that regulate gene expression during stress response, e.g. viral infection. in mammalian cells assembly of sgs is dependent on the ras-gap sh3-domain-binding protein (g3bp). the c-terminal domain of the viral nonstructural protein 3 (nsp3) of semliki forest virus (sfv) forms a complex with mammalian g3bp and sequesters it into viral rna replication complexes in a manner that inhibits the formation of sgs. the binding domain of nsp3 to hsg3bp was ... | 2017 | 27693920 |
| differential methylation of the circular dna in geminiviral minichromosomes. | geminiviral minichromosomes were purified to explore epigenetic modifications. the levels of methylation in their covalently closed circular dna were examined with the help of methylation-dependent restriction (mdr). dna with 12 superhelical turns was preferentially modified, indicating minichromosomes with 12 nucleosomes leaving an open gap. mdr digestion yielded a specific product of genomic length, which was cloned and sanger-sequenced, or amplified following ligation-mediated rolling circle ... | 2016 | 27716464 |
| a population genetics perspective on geminivirus infection. | the selective accumulation of both dna components of a bipartite geminivirus, abutilon mosaic virus, was recorded during early systemic infection of nicotiana benthamiana plants. purified nuclei were diagnosed for viral dna using hybridization specific for dna a or dna b to detect these individual genome components either alone or both simultaneously by dual-color staining. although this virus needs both components for symptomatic infection, dna a alone was transported to upper leaves, where it ... | 2015 | 26378173 |
| early function of the abutilon mosaic virus ac2 gene as a replication brake. | the c2/ac2 genes of monopartite/bipartite geminiviruses of the genera begomovirus and curtovirus encode important pathogenicity factors with multiple functions described so far. a novel function of abutilon mosaic virus (abmv) ac2 as a replication brake is described, utilizing transgenic plants with dimeric inserts of dna b or with a reporter construct to express green fluorescent protein (gfp). their replicational release upon abmv superinfection or the individual and combined expression of epi ... | 2015 | 25589661 |
| form follows function in geminiviral minichromosome architecture. | a comprehensive survey on the viral minichromosomes of the begomoviruses abutilon mosaic virus, tomato yellow leaf curl sardinia virus, african cassava mosaic virus, indian cassava mosaic virus (family geminiviridae) during the course of infections in nicotiana benthamiana is summarized. using optimized one-dimensional and two-dimensional gel systems combined with blot hybridization and a standardized evaluation, discrete and heterogeneous virus-specific signals with different dna forms were com ... | 2015 | 25445344 |
| evolutionary liberties of the abutilon mosaic virus cluster. | two new strains of abutilon mosaic virus (abmv; geminiviridae) from germany (stuttgart) and france (paris) have been characterized by circomics, direct pyrosequencing of rolling circle amplification (rca) products, as well as conventional cloning and sanger sequencing. rca combined with an analysis of restriction fragment length polymorphisms confirmed the completeness of the sequence determination and a close relationship of both isolates for dna a with 99 % nucleotide sequence identity. phylog ... | 2015 | 25315632 |
| molecular evidence for the occurrence of abutilon mosaic virus, a new world begomovirus in india. | during an investigation in the year 2010, on the weed reservoir of begomovirus, abutilon pictum showing bright yellow mosaic symptoms was observed in udhagamandalam, tamil nadu, india. the complete bipartite genome of a begomovirus was cloned and sequenced which revealed association of abutilon mosaic virus (abmv). nicotiana benthamiana plants inoculated biolistically with the concatemers generated through rolling circle amplification of the cloned dnas were asymptomatic; however three out of ni ... | 2013 | 24426288 |