| nucleic acid biosynthesis in rat embryo cells infected with x14 or h-1 parvovirus. | nucleic acid biosynthesis was studied in rat embryo cell (rec) cultures 48 hours after infection with x14 or h-1 parvovirus. the incorporation of 14c-formate and 6-(14c-orotic acid into purines and pyrimidines of various was lowered after infection with these parvoviruses. 14c-formate incorporation into acid-soluble thymine was greatly inhibited in h-1 virus-infected cells whereas it was slightly inhibited in x14 virus-infected cells. these results suggest that x14 virus-infected cells can carry ... | 1977 | 18918 |
| specific binding sites for a parvovirus, minute virus of mice, on cultured mouse cells. | the early interactions between parvoviruses and host cells have not been extensively described previously. in this study we have characterized some aspects of viral binding to the cell surface and demonstrated the existence of specific cellular receptor sites for minute virus of mice (mvm) on two murine cell lines that are permissive for viral growth. the interaction had a ph optimum of 7.0 to 7.2, and both the rate and extent of the reactions were slightly affected by temperature. mouse a-9 cel ... | 1977 | 20517 |
| isolation of nucleoprotein from the parvovirus krv. | | 1978 | 23606 |
| pyrimidine nucleotide synthesis in rat embryo cells infected with x14 or h-1 parvovirus. | the activity of some enzymes involved in pyrimidine nucleotide synthesis was studied in rat embryo cell (rec) cultures infected with x14 or h-1 parvovirus. dcmp aminohydrolase activity of the infected cells was 64--121% greater than that of the mock-infected cells. dtmp synthetase activity was 18% greater in x14 virus-infected cells and 34% lower in h-1 virus-infected cells. these results suggest some differences in the infected cells, as regards the biosynthesis of dtmp. orotate phosphoribosylt ... | 1978 | 26195 |
| h-1 and x14 parvovirus antibodies in women with abortions or still-births. | antibodies against h-1 and x14 parvoviruses were found 13.71 and 9.14%, respectively, of 350 sera from women affected by repeated abortions or still-births. on the contrary, only 2.66 and 1.66% of 300 control sera were positive to the two viruses. the difference in the incidence of antibodies appears highly significant (p greater than 0.001). therefore a possible role of the parvoviruses in inducing abortions or still-births in humans is suggested. | 1978 | 30268 |
| the pathogenicity of avian mycoplasmas. | based on literature data and own experiences the author gives an outlook about pathogenicity of avian mycoplasmas. in chickens and turkeys m. gallisepticum and m. synoviae (in addition to it m. meleagridis exclusively in turkeys) are the most important mycoplasmas producing respiratory disease, inflamation of synovial membranes and other lesions. their pathogenic effect is very much influenced by dose of agent, route of entry of microorganism, age of birds, virulence and tropism of organism as w ... | 1979 | 44612 |
| parvovirus-like particles in human sera. | a parvovirus-like antigen has been found in sera of nine healthy blood-donors and two patients. its pathogenicity is unknown, but 30% of adults possess specific antibody. the new agent can be confused with hepatitis-b antigen both morphologically and serologically. | 1975 | 46024 |
| letter: parvovirus-like particles in human faeces. | | 1975 | 47121 |
| letter: parvovirus-like particles in human sera. | | 1975 | 47477 |
| parvoviruses associated with diarrhea in calves. | | 1978 | 81199 |
| immunofluorescent studies of the local immune response in the mammary glands of rats. | two irritants, phosphate-buffered saline and alcohol, and antigens including killed brucella abortus, live b. abortus, staphylococcus aureus, and rat parvovirus were separately infused into rat mammary glands during pregnancy, and by using immunofluorescent techniques, the numbers of immunoglobulin-containing cells in glands during lactation and involution were determined. the study provided basic information on the local immune response of the mammary gland to antigens of various types. in all ... | 1979 | 106012 |
| [isolation of parvovirus from stillborn piglets in relation to the extensive occurrence of pregnancy disorders in sows]. | | 1979 | 106511 |
| porcine parvovirus: frequency of naturally occurring transplacental infection and viral contamination of fetal porcine kidney cell cultures. | the frequency of naturally occurring transplacental infection of swine with porcine parvovirus (ppv) and one of the possible consequences of such infection--the presence of ppv in cell cultures prepared from fetal tissues--were investigated. transplacental infection was indicated by the presence of high titers of hemagglutination inhibiting (hi) antibody for ppv in serums of 0-day-old, hysterectomy-derived, colostrum-deprived pigs of 3 of 82 litters. all letters were farm-raised dams. moreover, ... | 1975 | 163603 |
| ultrastructural studies of h-1 parvovirus replication. i. cytopathology produced in human nb epithelial cells and hamster embryo fibroblasts. | | 1975 | 167522 |
| murine virus susceptibility of cell cultures of mouse, rat, hamster, monkey, and human origin. | studies were initiated to determine the practicality of using various tissue cultures for the propagation of murine viruses isolated from laboratory animals. the cytopathogenic effects of 10 murine viruses known to cause disease in laboratory rodents were compared in monolayer cultures of l929, bhk-21, wi-38, bsc-1, and vero cells. the susceptibility of primary hamster embryo, hamster kidney, mouse embryo, mouse kidney, and rat embryo cell cultures was also tested. seven of the viruses produced ... | 1975 | 168428 |
| hepatitis a. perspectives and recent advances. | the basis for the epidemiologic and etiologic differentiation of two major forms of viral hepatitis, hepatitis a and b, was established in a series of studies undertaken between 1930 and 1970. final recovery and visualization of the presumed etiologic agent of hepatitis a was not, however, accomplished until the technique of immune electron microscopy was applied to the examination of specimen materials collected from individuals in the early acute stages of infection. morphologically homogeneou ... | 1975 | 174435 |
| prevalence of antibodies to porcine enteroviruses and porcine parvovirus in body fluids of fetal pigs from small vs large litters. | | 1976 | 175042 |
| ultrastructural studies of h-1 parvovirus replication. iii. intracellular localization of viral antigens with immunocytochrome c. | | 1976 | 178512 |
| separation and properties of enterovirus and reovirus recovered from a fecal sample of calf with diarrhea. | in april, 1971, a disease with pyrexia and diarrhea as main symptoms broke out collectively among calves. fecal samples were collected from calves involved and inoculated into bovine kidney (bk) cell cultures. as a result, the diarrheal feces of one calf were suspected to contain two agents simultaneously. one agent (c-121 e strain) was isolated from the primary infected bk cell culture fluid by terminal dilution passages. it had been predominant in replication and shown a cytopathic effect whic ... | 1976 | 181679 |
| the parvoviruses. | | 1976 | 181899 |
| isolation and structural characterization of monomeric and dimeric forms of replicative intermediates of kilham rat virus dna. | two virus-specific species of newly synthesized dna were isolated from rat fibroblast cell cultures infected with the kilham rat virus (rv). these two dna species were purified; their behavior on hydroxyapatite chromatography and their sedimentation coefficients in sucrose gradients were determined. one of the two species corresponds to the linear double-stranded form of the rv dna, and the other corresponds to the dimeric duplex form. after denaturation, a fraction of both species showed an int ... | 1976 | 185420 |
| isolation of aleutian disease virus of mink in cell culture. | aleutian disease virus, the causative agent of a persistent infection in mink, was isolated in a continuous line of feline renal cells when the cultures were maintained at reduced temperature (31.8 degrees). after serial in vitro passage of the virus at this temperature it had an optimum replication temperature of 37 degrees. an immunofluorescence focus assay was found to be suitable for virus quantitation. the cultured virus reproduced aleutian disease in mink, and the virus could be reisolated ... | 1977 | 192696 |
| characterization of the protein and nucleic acid of aleutian disease virus. | aleutian disease virus (adv) was extracted and purified from infected mink. nucleic acid extracted from the virus was examined in an electron microscope. three different sizes of molecule, with approximate lengths of 1.2, 0.55, and 0.25 micron, were observed. the ratios of the large molecules to the small molecules were similar in all the particles prepared under different conditions. equilibrium cscl density gradient centrifugation showed that adv nucleic acid had a buoyant density of 1.733 g/c ... | 1977 | 196113 |
| [modification of chromosome aberrations caused by kilham virus in rat embryo cells]. | mutagenic effect of kilham virus on the frequency of chromosome aberrations in the cells of primary and continous rat embryo cultures and the modification effect of cadmium salt on the mutagenic potential of this virus was studied. the frequency of chromosome aberrations increased in the primary rat embryo culture after kilham virus enfection. rat embryo culture chronically infected with kilham virus did not differ from control continuous cells in the frequency level of chromosome aberrations. i ... | 1977 | 199529 |
| transmissible gastroenteritis: demonstration of the virus from field specimens by means of cell culture and pig inoculation. | isolation of transmissible gastroenteritis virus was attempted from segments of jejunum collected from piglets submitted for diagnosis of transmissible gastroenteritis. the virus was isolated more frequently in susceptible piglets than in pig kidney or pig thyroid cells. practically, both cell systems were equally capable of demonstrating the virus when the tissue suspensions were sonicated. the pig thyroid cells prepared with glands collected from minimal disease pigs were preferred to the pig ... | 1977 | 200316 |
| cellular changes in primary rabbit kidney cells resembling those caused by certain viruses. | changes suggestive of being caused by a virus have recently been observed in primary rabbit kidney cells. in their nature and development they resembled those of parvoviruses. the changes were found in cultures from rabbits from various sources within and outside the netherlands. if the changes are caused by a viral agent, this agent is most probably of rabbit origin, as exogenous infection, for example through trypsin, could almost certainly be excluded. however, no virus has yet been isolated ... | 1976 | 201526 |
| infectious agents associated with fetal and early neonatal death and abortion in swine. | laboratory examination of specimens from 824 porcine abortions over a 6-year period produced 320 (38.8%) diagnoses. viruses were involved in 22% and bacteria in 16.5% of all cases. enterovirus was the virus isolated most often (10.9%), followed by parvovirus (4.9%), reovirus (4.4%), pseudorabies virus (1%), and adenovirus (0.8%). leptospirosis was the bacterial infection most commonly diagnosed (9.8%), with numerous miscellaneous bacterial and fungal infections making up the remainder (7.0%) of ... | 1978 | 203567 |
| persistent infection of a human lymphocyte cell line (molt-4) with the kilham rat virus. | inoculation of a human thymus-derived lymphocyte cell line (molt-4) with the kilham rat virus resulted in persistently infected cultures, that released infectious virus for periods up to seven months. at any time following infection, a small percentage of the cells were positive for rat virus antigens as determined by immunofluorescene assay. | 1978 | 203637 |
| human lymphoblastoid cells as hosts for parvoviruses h-1 and rat virus. | a human t-cell line (molt-4) was shown by viral hemagglutination and infectivity assays to support the replication of rat virus (rv) and h-1 virus. in addition, h-1 virus, but not rv, multiplied in two human b-cell lines, av-1 and nc-37. the ability to bind radioactively labeled rv was demonstrated for each of the cell lines, but viral adsorption occurred to a greater degree with molt-4 cells than with either av-1 or nc-37 cells. after challenge with rv, virus-specific antigens were detected in ... | 1978 | 203718 |
| rotavirus and winter gastro-enteritis in white south african infants. | in a microbiological investigation of winter gastro-enteritis in 23 white children rotaviruses were found in 14 (61%) and parvovirus-like particles in 1 (4%). bacteriological examination of stools from 11 of these patients yielded one isolate of salmonella eastbourne, but no enterotoxigenic or invasive bacteria were found. rotavirus appears to be the main aetiological agent of acute winter gastro-enteritis in white infants. | 1977 | 204064 |
| multiple buoyant densities of hepatitis a virus in cesium chloride gradients. | hepatitis a virus (hav) recovered from stools of human cases of hepatitis a and from stools of chimpanzees experimentally infected with hav was shown to possess multiple buoyant densities in cscl gradients. the greatest proportion of hav was most frequently found at a buoyant density of 1.32-1.34 g/cm3, however, large proportions of hav were also frequently found at higher densities, including 1.36-1.37, 1.40-1.42, and 1.45-1.48 g/cm3. these findings are consistent with the notion that hav may b ... | 1977 | 204743 |
| characterization and classification of virus particles associated with hepatitis a. i. size, density, and sedimentation. | virus-like particles were purified from stools of patients in an epidemic of hepatitis a in germany. when reference ms-1 chimpanzee pre-inoculation and convalescent sera were used, the close serological relationship of the purified particles to well-known isolates of hepatitis a could be established. on the other hand, the physicochemical characteristics of the particles were determined in parallel to the characteristics of a marker parvovirus (luiii) and a marker picornavirus (poliovirus type 2 ... | 1978 | 206730 |
| characterization and classification of virus particles associated with hepatitis a. ii. type and configuration of nucleic acid. | virus particles banding at 1.34 g/ml in cscl and sedimenting at 160s in sucrose gradients were isolated from fecal specimens of patients suffering from hepatitis. in the presence of 4 m urea and about 90% formamide, these particles released linear nucleic acid molecules of the kinked appearance characteristic of single-stranded rna or single-stranded dna. they could be distinguished from the nucleic acid of phage lambda added to the preparation as a marker for double-stranded configuration. expe ... | 1978 | 206731 |
| studies on the replication of krv single-stranded linear dna. | the autonomous parvovirus, kilham rat virus (krv), is composed of three structural proteins (salzman & white, 1970) and a single molecule of dna. the dna molecule is linear and single-stranded (ss). it is believed to have both 3' and 5'-terminal palindromic sequences (salzman, 1977). replication of the ss dna may involve double-stranded (ds) dna intermediates of one unit length as found in the virion or multiple length concatamers (salzman & white, 1973; gunther & may, 1976; lavelle & li, 1977). ... | 1978 | 207822 |
| growth of an autonomously replicating parvovirus (feline panleukopenia): kinetics and morphogenesis. | | 1978 | 208485 |
| the use of polyethylene glycol in concentration and purification of several bovine viruses. | the polyethylene glycol (peg) precipitation method was used for the concentration and purification of eight bovine viruses. good results were obtained from four viruses, parainfluenza--3 virus, bovine enterovirus, bovine adenovirus, and bovine parvovirus. no satisfactory results of concentration were obtained from bovine reovirus, bovine viral diarrhea virus, and infectious bovine rhinotracheitis virus. the failure of concentration of the four viruses seems to be ascribed rather to the resuspend ... | 1978 | 211444 |
| in vitro synthesis of double-stranded dna from the kilham rat virus single-stranded dna genome. | double-stranded, full-length linear dna was synthesized in vitro by using single-stranded linear dna as a self-priming template from the parvovirus kilham rat virus and escherichia coli dna polymerase "large fragment" as the polymerizing enzyme. to ascertain the order of the synthesis of the cleavage fragments and to assess the accuracy of the in vitro synthesis, restriction endonuclease cleavage sites with known recognition sequences were mapped on the dna. comparing the cleavage pattern of the ... | 1978 | 212593 |
| replication of nondefective parvoviruses: lack of a virion-associated dna polymerase. | we have examined four of the nondefective parvoviruses for an associated dna polymerase. virions were purified from neuraminidase-treated infected-cell lysates by isopycnic centrifugation in cscl or from infected cell material by cacl(2) precipitation and centrifugation through sucrose into cscl. preparations of bovine parvovirus or kilham rat virus obtained by the former procedure contained dna polymerase activity but were not free of contaminating cellular proteins. the latter method produced ... | 1978 | 212603 |
| [helper viruses of adeno-associated virus type 4 replication]. | in replication of adeno-associated virus type 4 (aav-4) the helper function may be performed by a non-defective virus from the same group of parvoviruses (kilham virus). the synthesis of aav-4 antigen was observed in a pig embryo kidney cell line, spev, chronically infected with kilham virus, strain rv-13, 45--52 passages. a one-day-old spev-kilham culture was infected with aav-4. the aav-4 antigen was detected by immunofluorescence at 6, 8, 12, 18 hours, 2, 3, 4, and 5 days after inoculation. d ... | 1979 | 217178 |
| parvovirus rna transcripts containing sequences not present in mature mrna: a method for isolation of putative mrna precursor sequences. | we report here a method of rna preparation that may enrich for precursor rna sequences and the results of an investigation of adeno-associated virus (aav) rna transcription that used this method. whole cells were lysed with detergent and high salt and separated into supernatant and pellet (crude chromatin) fractions. these fractions were then separately deproteinized by proteolytic digestion and phenol extractions. dna was removed from the preparation by two cycles of pancreatic dnase digestion ... | 1979 | 218213 |
| isolation and identification of a bovine adenovirus type 3 with an adenovirus-associated virus. | a bovine adenovirus with agglutinating activity was isolated from feedlot calves and classified as serotype 3. the agglutinating activity was shown to be the property of an adenovirus-associated virus (aav). the aav was isolated from the bovine adenovirus by isopycnic centrifugation in cscl; the aav had a density of 1.4 g/cm2. this aav is serologically related to bovine aav-tr-15, but is distinct from bovine parvovirus-1 and primate aav types 1 to 4, using counterimmunoelectrophoresis and hemagg ... | 1978 | 219733 |
| [structure of inclusion bodies and electron microscopic virus detection in naturally occurring inclusion body hepatitis in chickens]. | electron microscopy was used to examine the liver of chickens with spontaneous inclusion body hepatitis. eosinophilic inclusion bodies only were established from two flocks, mainly amphophilic from one flock, and primarily basophilic from another two flocks. eosinophilic inclusion bodies were predominant in broiler chickens with dystrophic fatty degeneration of the liver, while basophile inclusion bodies were recorded primarily from parental or laying-hen chickens with reduced metabolic stress o ... | 1978 | 219796 |
| studies of respiratory disease in random-source laboratory dogs: viral infections in unconditioned dogs. | a prospective study was conducted to identify the viruses causing respiratory diseases in unconditioned, random-source dogs. during the quarantine period, respiratory disease occurred in 86 of 167 (52%) dogs, and 34 (21%) died. most affected dogs had a distemper-like illness which required extensive and prolonged care. histopathologic studies confirmed the diagnosis of canine distemper in 10 of 12 (83%) fatal infections examined. sixty-seven of 91 (74%) dogs which arrived without canine distempe ... | 1979 | 220463 |
| defective-interfering particles of the human parvovirus adeno-associated virus. | | 1979 | 220782 |
| structure of the 3' hairpin termini of four rodent parvovirus genomes: nucleotide sequence homology at origins of dna replication. | the nucleotide sequences of the 3' termini of the dna from four autonomous rodent parvoviruses have been determined. the terminus of each genome exists as a y-shaped hairpin structure involving 115 or 116 nucleotides. the sequence of this region of dna is highly conserved and shows no evidence of internal sequence heterogeneity, a characteristic which is observed in the terminal nucleotide sequence of the helper-dependent, adeno-associated viruses (berns et al., 1978a). the implications of these ... | 1979 | 225040 |
| nucleotide sequence of the self-priming 3' terminus of the single-stranded dna extracted from the parvovirus kilham rat virus. | the parvovirus genome is a linear, single-stranded dna molecule with double-stranded hairpin termini. the 3' terminus can serve in vitro as a self-primer for the synthesis of a double-stranded viral dna intermediate. we have sequenced the nucleotides in the 3' terminus and propose a model for the secondary structure of the terminus and the in vitro origin of replication for the complementary viral dna strand. | 1979 | 225552 |
| expression of the autonomous parvovirus h1 genome: evidence for a single transcriptional unit and multiple spliced polyadenylated transcripts. | we identified viral transcripts in parvovirus h1-infected rodent cells using the s1 nuclease mapping technique of berk and sharp (1977, 1978). the most abundant viral transcript, present in both nucleus and cytoplasm, is approximately 2.8 kb long and represents about 56% of the viral genome. less abundant viral transcripts of 3.0, 1.45 and 1.30 kb, and possibly other minor viral transcripts, are also detected in nuclear and cytoplasmic fractions. in contrast, a prominent 4.7 kb viral transcript ... | 1979 | 226267 |
| canine parvovirus--a new disease. | | 1979 | 229621 |
| spliced adenovirus-associated virus rna. | we describe the structure of cytoplasmic rna species transcribed from the dna of adenovirus-associated virus, a defective parvovirus. the rna was hybridized with minus strand template dna and visualized in the electron microscope. alternatively, the dna.rna duplex molecules were digested with nuclease s1 or escherichia coli exonuclease vii and analyzed by agarose gel electrophoresis. a set of rna species was observed with 5' terminal at map positions 5, 13, 19, or 39 and a 3' terminus and poly(a ... | 1979 | 230481 |
| inhibition of the replication of parvovirus x14 by 5-iodo-2'-deoxyuridine pre-treatment of cell cultures. | pre-treatment of rat embryo cell cultures with 5-iodo-2'-deoxyuridine (idurd) inhibits the replication of parvovirus x14. reduced yields of haemagglutinating and infectious particles were observed. adsorption of virus to cells was not affected, but both virus protein and dna synthesis were inhibited. fewer cells were capable of supporting protein or antigen synthesis as determined by immunofluorescence. virus-specific dna was detected in idurd pre-treated cells, but the amount synthesized was co ... | 1979 | 231085 |
| incomplete genomes of the parvovirus minute virus of mice: selective conservation of genome termini, including the origin for dna replication. | deletion mutants of minute virus of mice arising during a single high-multiplicity passage and after serial undiluted passage have been isolated, and the incomplete viral genomes contained therein have been analyzed. the dna isolated from incomplete virions derived from a single high-multiplicity passage was heterogeneous, ranging in size from 15 to 70% of the intact viral genome, with an average molecular length of approximately, 2,000 nucleotides. two distinct types of molecules, designated as ... | 1979 | 232178 |
| characterization of the deoxyribonucleic acid polymerase associated with kilham rat virus. | purified preparations of the parvovirus, kilham rat virus, have associated with them a protein with dna polymerase activity. the enzyme has been separated from the other two or three viral proteins and purified 63-fold. the viral associated enzyme was found in a single peak of dna polymerase activity after chromatography on deae-cellulose, dna-cellulose, and phosphocellulose columns. it shares some properties in common with the host cellular dna polymerases, described in the preceding paper (sal ... | 1975 | 237924 |
| development of sensitive methods for detection of latent kilham virus infection of rats. | the developed system of procedures, including preliminary selection of rats according to the presence of 19 s antibody in the blood serum, and the use of the most sensitive methods has increased considerably the incidence of detection of kilham virus (kv) in organs of latently infected rats. to isolate and establish kv strains from the organs of rats in which the specific antigen was detected by immunofluorescence, at least 5-9 passages in rat embryo cell cultures or in newborn syrian hamsters w ... | 1975 | 241235 |
| morphological methods of kilham virus detection. | morphological investigations on organs from kilham virus (kv)-infected syrian hamsters revealed acute glomerulonephritis with a clear-cut haemorrhagic syndrome. no pathological changes were found in internal organs from kv-infected rats. | 1975 | 241237 |
| isolation of porcine parvovirus from aborted piglets. | | 1977 | 275675 |
| mechanism for circularization of linear dnas: circular parvovirus mvm dna is formed by a "noose" sliding in a "lasso"-like dna structure. | during an electron-microscopic survey with the aim of identifying the parvovirus mvm transcription template, we observed previously unidentified structures of mvm dna in lysates of virus-infected cells. these included double-stranded "lasso"-like structures and relaxed circles. both structures were of unit length mvm dna, indicating that they were not intermediates formed during replication; they each represented about 5% of the total nuclear mvm dna. the proportion of these structures was uncha ... | 1979 | 291964 |
| parvovirus infection in dogs. | | 1979 | 292917 |
| covalent association of protein with replicative form dna of parvovirus h-1. | the double-stranded replicative form (rf) dna of the autonomous parvovirus h-1 can be isolated from infected cells in a covalent complex with protein. the protein is present on most or all of the rf dna, including actively replicating molecules, and is associated with the 5'-terminal endonuclease hae iii fragments of both the viral and complementary strands of rf. the size of the protein is estimated to be 60,000-70,000 daltons from its effect on buoyant density of dna. dna with covalently bound ... | 1979 | 293659 |
| isolation of a parvovirus from dogs with enteritis. | | 1979 | 295110 |
| canine hemorrhagic enteritis: detection of viral particles by electron microscopy. | at necropsy, several dogs which died showing symptoms of hemorrhagic diarrhea, had significant lesions of the mucosa that were found especially in the duodenum and upper part of the small bowel. study of ultrathin sections from the diseased mucosa revealed particles resembling parvoviruses in altered nuclei of cells of the intestinal crypts. electron microscopic examination of intestinal contents by negative staining has shown the presence of many viral particles which have a diameter of 24 nm a ... | 1979 | 316320 |
| [improved methods of detecting latent parvovirus infection in rats]. | | 1977 | 331735 |
| pathogenesis of porcine parvovirus infection: pathology and immunofluorescence in the foetus. | | 1977 | 332722 |
| ultrastructural studies of h-1 parvovirus replication. vi. simultaneous autoradiographic and immunochemical intranuclear localization of viral dna synthesis and protein accumulation. | the localization of h-1 viral replicative-form double-stranded dna and progeny single-stranded dna replication in parasynchronously infected, simian virus 40-transformed newborn human kidney cells was studied with high-resolution electron microscope autoradiography (80-nm silver grains). we analyzed wild-type h-1 and ts1 h-1 (a conditional mutant defective in progeny single-stranded dna synthesis). the proportion of the total dna synthesis that was viral was estimated to be >90% by comparing the ... | 1978 | 340710 |
| viruses and diarrhea--a review. | a critical analysis of the literature shows that only a few viral agents have been proved by accepted criteria to cause acute gastroenteritis. rotavirus, of the rna family reoviridae, is the cause of most childhood gastroenteritis. the parvovirus-like agents (norwalk, hawaii, mc, and w) are proved agents of epidemic gastroenteritis. there is good evidence that the marcy, fs, and niigata agents were the causes of gastroenteritis outbreaks. thirty percent of all acute gastroenteritis episodes in l ... | 1978 | 343580 |
| demonstration of parvovirus in canadian swine and antigenic relationships with isolates from other countries. | a canadian isolate of porcine parvovirus, isolated from cultured pig thyroid cells, was shown to be antigenically indistinguishable from a british (59e/63) and a german (g10/1) strain when treated by the modified direct complement-fixation, the hemagglutination-inhibition and the fluorescent antibody tests. these tests also revealed that antibodies to parvoviruses were detectable in a large proportion of the conventionally raised pigs in the provinces of quebec and ontario. cell cultures, prepar ... | 1978 | 356941 |
| isolation of parvovirus from kidney cell cultures of gnotobiotic piglets. | | 1979 | 394537 |
| interaction in replication between goose parvovirus strain b and duck plague herpesvirus. | the goose parvovirus strain b was found to replicate only in cells with dna synthesis. cells in the stationary phase of dna synthesis or maintained under 2 mm thymidine (tdr) in excess were refractive to the parvovirus infection. however, stationary phase cells could be rendered to produce progeny paroviruses by herpesvirus superinfection. in reverse, the replication of the herpesvirus was significantly inhibited by parvovirus preinfection of cells. | 1979 | 426633 |
| prenatal infection following maternal exposure to porcine parvovirus on either the seventh or fourteenth day of gestation. | intranasal and oral exposure of two gilts to porcine parvovirus on either the seventh or 14th day of gestation resulted in prenatal infection. normal appearing fetuses and necrotic remnants of what were believed embryos and extraembryonic membranes were found when the gilts were necropsied seven weeks after exposure. the presence of masses of porcine parvovirus antigen throughout necrotic tissues of six of seven embryos, but not in any of the nine normal appearing fetuses suggested that embryoni ... | 1979 | 427636 |
| effect of porcine parvovirus on development of fertilized pig eggs in vitro. | | 1979 | 435962 |
| effect of transferring parvovirus-infected fertilized pig eggs into seronegative gilts. | | 1979 | 435963 |
| sudden death in young dogs with myocarditis caused by parvovirus. | sudden death of pups in the 4- to 6-week age range has recently been occurring in western canada as a result of severe, primary, nonsuppurative myocarditis. at necropsy, the prominent macroscopic lesion was pulmonary edema, and microscopically, characteristic intranuclear inclusion bodies were found within cardiac myofibers in association with myocarditis. ultrastructurally, numerous small particles resembling parvoviruses were found within the intranuclear inclusion bodies, which were positive ... | 1979 | 438048 |
| myocarditis and enteritis in puppies associated with parvovirus. | | 1979 | 452332 |
| canine enteritis caused by a parvovirus-illinois. | | 1979 | 457568 |
| subfractionation of cscl-purified h-1 parvovirus on metrizamide gradients. | the different density classes of h-l parvovirus, collected within 30 hr of infection of par-asynchronous cultures, following the standard cscl purification step, have been shown to be heterogeneous. rebanding of the denser form (hf, p = 1.46 g/cm3) and the less dense form (lf, p = 1.42 g/cm3) of infectious virus in the nonionic density generating solute, metrizamide, showed that both hf and lf virus bands were heterogeneous in density. the infectivity banded with isotopically labeled virus prote ... | 1979 | 462819 |
| sequence of the 3' terminus of the genome from kilham rat virus, a nondefective parvovirus. | | 1979 | 462820 |
| efficacy of an inactivated virus vaccine for prevention of porcine parvovirus-induced reproductive failure. | gilts vaccinated im either once (4 gilts) or twice (2 gilts) with an acetylethyleneimine-inactivated porcine parvovirus (ppv) vaccine before they were bred were subsequently exposed intranasally and orally to virulent ppv at about the 40th day of gestation (from 37 to 43 days). at 2 weeks after vaccination, all had hemagglutination-inhibiting (hi) titers for ppv (from 20 to 80) which decreased by the time the immunity was challenged with virulent virus (from 10 to 40), but increased thereafter ( ... | 1979 | 464358 |
| isolation of bovine parvovirus type 1 in australia. | | 1979 | 464943 |
| isolation of rodent parvoviruses in australia. | | 1979 | 464945 |
| canine viral enteritis. ii. morphologic lesions in naturally occurring parvovirus infection. | the morphologic lesions associated with naturally occurring canine parvovirus infection are described. the disease is characterized by necrosis of crypt epithelium in the small intestine and of lymphoid tissue in peyers patches, lymph nodes, spleen and thymus. | 1979 | 467076 |
| a possible parvovirus associated with an epidemic gastroenteritis of dogs in canada. | | 1979 | 473507 |
| early events in parvovirus replication: lack of integration by minute virus of mice into host cell dna. | viral dna sequences were not detected in high-molecular-weight host dna until well after the onset of viral dna replication. | 1979 | 480460 |
| transcription of the bovine parvovirus genome in isolated nuclei. | transcription of the genome of the nondefective parvovirus bpv was examined in nuclei isolated from synchronized bovine fetal spleen cells. the relative levels of total rna polymerase and rna polymerase i, ii, and iii activities in nuclei isolated from bpv-infected and mock-infected cells were found to be similar throughout the course of infection. hybridization of rna synthesized in isolated nuceli indicated that bpv-specific rna synthesis began during the period of 8 to 12 h postinfection and ... | 1979 | 480472 |
| effect of inseminating seropositive gilts with semen containing porcine parvovirus. | | 1979 | 487052 |
| myocarditis in young dogs associated with a parvovirus-like agent. | | 1979 | 487357 |
| lesions of spontaneous canine viral enteritis. | spontaneous enteric disease characterized by hemorrhagic diarrhea and high mortality occurred in puppies from commercial kennels in three midwestern states. microscopic lesions resembling those of panleukopenia in cats were seen in the intestines. the predominant features were necrosis of crypt epithelium, collapse or dilation of crypt lumina and villous atrophy. viral particles morphologically resembling parvovirus were found in the feces by direct electron microscopy. the canine virus reacted ... | 1979 | 505892 |
| [variations in resistance of viruses from different groups to chemico-physical decontamination methods]. | the resistance of a total of 13 different viruses to some important chemico-physical influences was studied under uniform experimental conditions. stability in tape water, thermostability and sensitivity to anodic oxidation, gamma radiation, some virucidal substances and several commercial disinfectants were tested. in evaluating the results, an attempt is made to rank the viruses investigated according to their sensitivity. on average a bovine parvovirus, and also a reovirus and three enterovir ... | 1979 | 511342 |
| isolation of a parvovirus from dogs in britain. | | 1979 | 516348 |
| canine parvovirus isolated in uk. | | 1979 | 516351 |
| evidence for a parvovirus as the aetiologic agent in the myocarditis of puppies. | | 1979 | 518427 |
| parvovirus infection in maned wolves. | | 1979 | 521366 |
| porcine parvovirus infection in ontario: incidence and diagnosis in herds with reproductive failures. | | 1979 | 526907 |
| histopathologic evidence for parvovirus infection in dogs. | two dogs kenneled at a commercial establishment died of a disease manifested as severe hemorrhagic diarrhea. histopathologic findings in both dogs bore a striking resemblance to those typical of feline infectious enteritis. viral particles resembling members of the parvoviridae family were observed in the contents of the small intestine of one of the dogs. the disease was similar to the recently recognized canine gastroenteritis associated with a parvovirus-like agent. | 1979 | 528325 |
| [parvovirus infections in dogs in the netherlands]. | | 1979 | 531881 |
| parvovirus infection in dogs. | | 1979 | 532064 |
| vaccination against canine parvovirus. | | 1979 | 532070 |
| parvovirus infection and greyhounds. | | 1979 | 532077 |
| parvovirus infection treatment. | | 1979 | 532078 |
| parvovirus as a cause of enteritis and myocarditis in puppies. | a gastro-enteritis syndrome mimicking feline panleukopaenia was diagnosed in young dogs in the republic of south africa. parvovirus was demonstrated by electron microscopy in the faeces of these animals. in addition an acutely fatal, acute to sub-acute non-purulent interstitial myocarditis occurred in pups in the same area. histopathologically large basophilic intranuclear inclusion bodies were seen in the myocardium of these cases. the two syndromes generally occurred separately, but in two cas ... | 1979 | 553961 |
| embryonal carcinoma cells (and their somatic cell hybrids) are resistant to infection by the murine parvovirus mvm, which does infect other teratocarcinoma-derived cell lines. | minute virus of mice (mvm), a non-defective parvovirus, has been shown to infect cultures of non-pluripotent differentiated teratocarcinoma-derived cells, but pluripotent (and "nullipotent") embryonal carcinoma cells derived from the same teratocarcinoma resist mvn infection. somatic cell hybrids between an embryonal carcinoma line and friend erythroblastic leukemia cells are also resistant to mvm, even though friend cells are susceptible. among three blastocyst-derived lines tested, only one, a ... | 1977 | 558990 |
| kinetics of assembly of a parvovirus, minute virus of mice, in synchronized rat brain cells. | the rates of assembly of the three classes of particles of minute virus of mice were examined in synchronized rat brain cells by a combination of electron microscopy and biochemical techniques. we observed a burst of virus assembly beginning about 8 h after the end of cellular s phase. labeled thymidine incorporated into the 1.46 g/cm3 class of full virus particles was transferred almost quantitatively to the 1.42 g/cm3 class. the 1.46 g/cm3 virus appeared to be an immediate precursor to the 1.4 ... | 1977 | 559779 |