| the amino acid sequence of the ccgg recognizing dna methyltransferase m.bsufi: implications for the analysis of sequence recognition by cytosine dna methyltransferases. | the bacillus subtilis fi dna methyltransferase (m.bsufi) modifies the outer cytosine of the dna sequence ccgg, causing resistance against r.bsufi and r.mspi restriction. the m.bsufi gene was cloned and expressed in b.subtilis and escherichia coli. as derived from the nucleotide sequence, the m.bsufi protein has 409 amino acids, corresponding to a molecular mass of 46,918 daltons. including these data we have compared the nucleotide and amino acid sequences of different ccgg recognizing enzymes. ... | 1990 | 2108858 |
| bacillus subtilis phage spr codes for a dna methyltransferase with triple sequence specificity. | spr, a temperate bacillus subtilis phage, codes for a dna methyltransferase that can methylate the sequences ggcc (or ggcc) and ccgg at the cytosines indicated. we show here that it can also methylate the sequence cc(a/t)gg and protect it from cleavage with ecorii and apyi. this methylation can be seen in vivo as well as in vitro with purified spr methyltransferase. spr19 and spr83 are two mutant phages, defective in ggcc or ccgg methylation, respectively. these mutants have not lost their abili ... | 1987 | 3108859 |
| dna methyltransferase genes of bacillus subtilis phages: structural relatedness and gene expression. | the dna methyltransferase (mtase) genes of temperate bacillus subtilis phages phi 3t, rho 11 and sp beta were cloned and expressed in escherichia coli. each gene specifies a 47-kda1 protein, which modifies bsur (ggcc) and fnu4hi (gcngc) target sequences. transcription is controlled by phage promoters located on the cloned fragments. the direction of transcription and the approximate position of the mtase genes were determined. dna/dna hybridization experiments revealed close structural relatedne ... | 1985 | 3928442 |
| restriction and modification in bacillus subtilis: nucleotide sequence, functional organization and product of the dna methyltransferase gene of bacteriophage spr. | bacillus subtilis phage spr codes for a dna methyltransferase (mtase) which methylates the 5' cytosine in the sequence ggcc and both cytosines in the sequence ccgg. a 2126-bp fragment of spr dna containing the mtase gene has been sequenced. this fragment has only one significant open reading frame of 1347 bp, which corresponds to the mtase gene. within the sequence the mtase promoter has been defined by s1 mapping. the size of the spr mtase predicted from the deduced amino acid composition is 49 ... | 1984 | 6092231 |
| structure of the gene coding for the sequence-specific dna-methyltransferase of the b. subtilis phage spr. | the nucleotide sequence of the gene coding for the 5'-ggcc and 5'-ccgg specific dna methyltransferase of the bacillus subtilis phage spr was determined by the maxam-gilbert procedure. transcriptional and translational signals of the sequence were assigned with the help of s1 mapping and translation in e. coli minicells. the gene codes for a 49 kd polypeptide. the amino acid sequence of the spr methylase shows regions of homology with the sequence of the 5'-ggcc-specific bspri modification methyl ... | 1984 | 6096817 |
| restriction and modification in bacillus subtilis: expression of the cloned methyltransferase gene from b. subtilis phage spr in e. coli and b. subtilis. | expression of the spr methyltransferase gene from b. subtilis phage spr cloned into lambda and spp1 was studied by analyzing the sensitivity of the hybrid phage dnas to restriction by the enzymes haeiii, mspi, and hpaii. the following results were obtained: (1) the genes were expressed both in the homologous (b. subtilis) and heterologous (e. coli) host. (2) the specificity of the expression of the cloned gene was identical to that of the gene in spr. (3) expression depended on the orientation o ... | 1983 | 6222239 |