| staphylococcus aureus bacteriophages mediating the simultaneous lysogenic conversion of beta-lysin, staphylokinase and enterotoxin a: molecular mechanism of triple conversion. | a new group of serotype f bacteriophages of staphylococcus aureus has been found which mediates the simultaneous triple-lysogenic conversion of enterotoxin a, staphylokinase and beta-lysin. the phages were recovered fro methicillin-resistant strains of s. aureus isolated in irish hospitals between 1971 and 1988 and from strain ps42-d, which has been used as the propagating strain for the s. aureus typing phage 42d since before 1965. the molecular mechanism of triple conversion mediated by three ... | 1989 | 2533245 |
| characterization of staphylococci isolated from mastitic cows in spain. | a total of 57 gram-positive, catalase-positive cocci, considered etiological agents of clinical and subclinical bovine mastitis, were tested for glucose and mannitol fermentation, coagulase and thermonuclease production, sensitivity to lysostaphin, gelatin hydrolysis, lysozyme, phosphatase and egg yolk factor production, hemolytic properties, antibiotic sensitivity, susceptibility to human and bovine phages, and enterotoxin production. all 57 strains were identified as staphylococci. a good corr ... | 1980 | 7387155 |
| longitudinal study on the susceptibility to bacteriophages of staphylococcus aureus strains isolated from dairy farms in trinidad. | a 6-month longitudinal study was conducted on 30 dairy cows in early lactation and their human handlers on six farms across trinidad. weekly samples of bulk milk, composite milk and anterior nares and hand swabs from human handlers were collected and cultured for staphylococcus aureus on baird-parker agar (bpa). the susceptibility of s. aureus strains to bacteriophages and the relatedness of strains isolated over the study period were determined. sixty-three (51.2%) of 123 strains of s. aureus f ... | 1999 | 10574074 |
| relatedness of staphylococcus aureus strains isolated from milk and human handlers in dairy farms in trinidad. | the susceptibility of s. aureus strains isolated from milk and human handlers in dairy farms in trinidad to bacteriophages was determined. of the 110 strains isolated from bulk milk, 73 (66.4%) were typable either at routine test dilution (rtd) or 100-rdt compared to 108 (72.5%) of 149 strains from composite milk sensitive but the difference was not statistically significant (p > or = 0.05; chi 2). strains of s. aureus from human handlers had significantly (p < or = 0.05; chi 2) lower sensitivit ... | 1997 | 9451945 |
| characteristics of staphylococcus aureus strains isolated from bovine mastitic milk: bacteriophage and antimicrobial agent susceptibility, and enterotoxigenicity. | staphylococcus aureus strains isolated from bovine mastitic milk in trinidad were examined for their susceptibility to bacteriophages and antimicrobial agents and their ability to produce enterotoxins. phage 42d was used to screen for bovine strains of s. aureus in milk. of 250 strains tested, 224 (89.6%) were sensitive to phages in the international phage set (ips), 85 (34.0%) were resistant to antimicrobial agents and 134 (53.6%) were enterotoxigenic. strains lysed by phages in various groups ... | 1995 | 8553706 |
| purification and characterization of the bacterial plasminogen activator staphylokinase secreted by a recombinant bacillus subtilis. | a gene coding for the bacterial plasminogen activator staphylokinase (sak) was cloned from staphylococcus aureus bacteriophage 42d into an exoprotease reduced mutant strain of bacillus subtilis (1). yields of up to 50 mg sak per litre of culture supernatant were obtained depending on the medium used. sak purified by ion exchange chromatography and gel filtration had a specific activity of 16,000 units/mg protein. isoelectric focusing of the purified sak revealed heterogeneity with respect to the ... | 1988 | 3146168 |
| cloning and expression in escherichia coli, bacillus subtilis, and streptococcus sanguis of a gene for staphylokinase--a bacterial plasminogen activator. | the gene coding for the bacterial plasminogen activator staphylokinase was cloned from the staphylococcus aureus phage 42d, a serogroup f phage used for lysotyping, onto the standard escherichia coli plasmid vector pacyc184. the coding and flanking sequences of the sak42d gene were largely identical to those of a sak gene cloned from the serologically different s. aureus phage søc (sako and tsuchida 1983). subcloning of a 2.5 kb phage 42d dna fragment onto plasmid pgb3631 allowed the sak42d gene ... | 1987 | 3123893 |
| expression and secretion of interferon-alpha 1 by streptomyces lividans: use of staphylokinase signals and amplification of a neo gene. | a gene coding for mature human interferon, ifn-alpha 1, fused to the expression and secretion signals of a staphylokinase gene (sak) derived from staphylococcus aureus phage 42d, was inserted into the streptomyces promoter probe vector pij487. streptomyces lividans transformed with the recombinant plasmid (pmg341) secreted biologically active ifn-alpha 1 into the culture medium. expression of the ifn-alpha 1 gene was at least on the translational level directed by the sak signals since numerous ... | 1988 | 2851494 |