| [plasmid transduction using the d3112deltah miniphage in pseudomonas aeruginosa cells]. | plasmid dna transduction with mini-d3112 delta h, deletion derivative of phage d3112, which lost the genes essential for phage growth but retained the sites required for transposition and packaging was studied. unlike d3112, mini-d3112 delta h element can transduce plasmids and plasmid markers at frequencies of 10(-5)-10(-8) in rec+ cells of pseudomonas aeruginosa. plasmids r1162 and r388 of the size smaller than phage genome were transduced intact. large plasmids, like rp4 and r151, were delete ... | 1992 | 1468641 |
| characterization of the pseudomonas aeruginosa transposable phage d3112 [corrected] left-end regulatory region. | the nucleotide sequence (2682 bp) of the left end of the mu-like transposable bacteriophage d3112 cts15 from pseudomonas aeruginosa was determined. a 720 bp open reading frame (orf) is located on the bottom strand (positions 892-173), potentially encoding a polypeptide of 240 residues (mr = 26,329). specific binding of escherichia coli integration host factor (ihf) to a site located 907-922 bp from the d3112 left end suggests the existence of a p. aeruginosa ihf and its role, as in mu, in the re ... | 1991 | 1846565 |
| [genetic control of morphogenesis of pseudomonas aeruginosa transposable phage d3112]. | the influence of ts mutations in the early and late genes of transposable phage d3112 on phage morphogenesis was studied. the mutations in the early genes a, b and c were shown to suppress morphogenesis of d3112. six genes (d, e, f, g, h and i), located from 14 to 29 kbp of the phage physical map, control morphogenesis of phage head. five genes (j, k, l, m and n), clustered in the 29-36 kbp region of the map, control morphogenesis of tail. the similarity of genetic organization of the escherichi ... | 1989 | 2517491 |
| mini-d3112 bacteriophage transposable elements for genetic analysis of pseudomonas aeruginosa. | small bacteriophage d3112 transposable elements deleted for most of the phage-lytic functions while retaining the sites required for transposition and packaging were constructed to facilitate genetic studies in pseudomonas aeruginosa. these mini-d derivatives were constructed with the terminal 1.85 kilobases (kb) of the phage left end and 1.4 kb of the phage right end and either the tn5 kanamycin resistance or the psc101 (pbr322) tetracycline resistance determinant. thermally induced lysates of ... | 1989 | 2544562 |
| in vivo cloning of pseudomonas aeruginosa genes with mini-d3112 transposable bacteriophage. | the transposition properties of the pseudomonas aeruginosa mutator bacteriophage d3112 were exploited to develop an in vivo cloning system. mini-d replicon derivatives of d3112 were constructed by incorporating broad host range plasmid replicons between short terminal d3112 sequences. these elements were made with small replication regions from the rk2, sa, and pvs1 plasmids and selectable genes for tetracycline, carbenicillin, kanamycin, and gentamicin resistance. some of the mini-d replicons a ... | 1989 | 2544563 |
| [compatibility of transposable phages of escherichia coli and pseudomonas aeruginosa. i. co-development of phages mu and d3112 and integration of phage d3112 into rp4::mu plasmid in pseudomonas aeruginosa cells]. | the possibility of using a model system (which included rp4::mu plasmid and d3112 phage in pseudomonas aeruginosa cells) for analysis of compatibility of transposable escherichia coli phage mu and p. aeruginosa phage d3112, as phages and transposons, was studied. no interaction was observed during the vegetative growth of phages. the majority of the hybrid rp4::mu plasmids lost the mu dna after insertion of d3112 into rp4::mu. the phenomenon was not a result of transposition immunity. we conside ... | 1988 | 2840341 |
| [interaction of heterologous bacteriophages: growth suppression of temperate pp56 phage of pseudomonas putida by the transposable phage d3112 of pseudomonas aeruginosa]. | we have found an inhibiting effect of hybrid rp4::d3112 plasmid (where d3112 is represented as genome of a transposable phage specific for pseudomonas aeruginosa) on the development of temperate p. putida phage pp56. the study of the effect has revealed a previously unknown locus (in the region 12-14.2 kb of the d3112 genome) which functions in the prophage state. the locus affects pp56 decreasing phage yield. mutants of pp56 insensitive to inhibition were found. | 1988 | 2843421 |
| [minigenomes of the transposable phage d3112 of pseudomonas aeruginosa and their properties]. | two derivatives of d3112 prophage with large internal deletions (mini-d3112) have been constructed. mini-d3112 delta h is about 3.5 kb long, containing the repressor c1 gene. mini-d3112 delta e is about 12 kb long, contains the c1 gene, several structural genes and replication gene a. these mini-d3112 are unable to replicate. however, they could replicate and maturated in the presence of the helper d3112cts. mini-d3112 mediate translocation of the gene argh from the chromosome into the r' plasmi ... | 1988 | 2843423 |
| [transcription of the genome of pseudomonas aeruginosa phage-transposon d3112 in an homologous host]. | in vivo transcription of pseudomonas aeruginosa transposable phage d3112 was studied. the 3h-labelled rna isolated from the lysogenic cells pao1 (d3112cts) after heat induction was hybridized with d3112 dna to estimate phage-specific rna. two main stages of d3112 transcription, including transcription of the early (first 6-8 min) and the late (after 8th min) genes, were revealed. the transcription rate of d3112 early genes achieves the maximum at 3-5 min, being reduced to the minimum at 6-8 min ... | 1988 | 2848750 |
| [genetic map of the transposable phage d3112 of pseudomonas aeruginosa]. | using a large group of newly isolated deletion mutants of prophage d3112 the location of all known mutations of d3112 phage was more precisely defined. the mutations affecting establishment of lysogenic state were mapped in two regions of the genome- 0-1.3 and 29-30 kb. the replicative a gene is mapped between 1.3 and 4.9 kb, the second replicative b gene being situated on the right of the a gene, between 4.9 and 9.4 kb. the c gene which is responsible for positive regulation of phage late genes ... | 1988 | 2855068 |
| [coupling and rec-independence of the processes of replication and transposition of pseudomonas aeruginosa phage d3112. the effect of the phage genes controlling the replication of dna of d3112]. | d3112 phage was shown to replicate via the process of coupled replication--transposition: the phage dna is not excised from the chromosome after prophage induction and new phage copies insert into many different sites. the transposition is controlled by two d3112 early genes--a (mapped in the 1.5-3 kbp region) and b (3-4.5 kbp), and requires intact attl site (involvement of the phage right end attr not studied). d3112 is capable to transpose rp4 plasmid into the chromosome; both the d3112 and rp ... | 1986 | 3015725 |
| [effective, plasmid rp4-dependent replication-transposition of dna of the transposable phage d3112 of pseudomonas aeruginosa in a heterologous escherichia coli system]. | the processes of replication and transposition of pseudomonas aeruginosa transposable phage d3112 in cells of escherichia coli (d3112) and e. coli (rp4::d3112) were studied. d3112 genome is a "silent cassette" ("conex-phage"--conditionally expressible) in e. coli cells incubated at 42 degrees c. two compulsory conditions for d3112 genome expression are incubation at 30 degrees c and the presence in cells of rp4 plasmid. processes of replication and transposition in e. coli are coupled. rp4 plasm ... | 1986 | 3021578 |
| [introduction of the hybrid plasmid rp4::d3112 into pseudomonas putida cells requires the presence of specific mutation in the phage genome]. | the wild type of d3112, a transposable phage of pseudomonas aeruginosa can not be introduced as a portion of the hybrid plasmid rp4::d3112 into pseudomonas putida cells. it is only possible when phage d3112 carries mutations designated lpc (lethal for p. putida and escherichia coli). analysis of heteroduplex molecules between dnas of phages d3112w+ and d3112lpc demonstrated the absence of nonhomology regions, which suggests that lpc is a point mutation. the lpc2 mutation was located within the i ... | 1984 | 6086454 |
| [pseudomonas aeruginosa bacteriophages with dna structure similar to the dna structure of mu1 phage. ii. evidence for similarity between d3112, b3, and b39 bacteriophages: analysis of dna splits by restriction endonucleases, isolation of d3112 and b3 recombinant phages]. | it is found that bacteriophages b3 and b39 specific for pseudomonas aeruginosa have the same genome structure as previously described phage d3112. on the right (s) end of their genomes a variable non-phage dna is located (approximately 0.9-2.5 kilobases for different phages). it is probable that this variable dna has its origin from different regions of bacterial chromosome. in genome of one of the phages, b3 phage, such variable dna (not more than 150 base pairs) was found on the left end of dn ... | 1980 | 6256259 |
| [transposition of the phage d3112 genome in escherichia coli cells]. | it has been demonstrated that the genome of phage d3112 of preudomonas aeruginosa can be transposed into escherichia coli chromosome as a component of the hybrid plasmid rp4 tcrkms::d3112. also, transposition of d3112 from e. coli (d3112) chromosome into rp4 plasmid occurs. the phage stimulates the chromosome mobilizing activity of rp4 plasmid, similar to other transposons. e. coli (rp4::d3112) cells were previously shown to form no colonies at 30 degrees c. auxotrophic mutants and mutants incap ... | 1983 | 6317518 |
| insertion and replication of the pseudomonas aeruginosa mutator phage d3112. | d3112 is a temperate bacteriophage of p. aeruginosa with heterogeneous sequences at one extremity of the virion dna molecule. infection of strain paol with phage d3112 results in a 40- to 65-fold increase in the frequency of ami mutants resistant to fluoroacetamide. nine ami::d3112 prophages have been mapped to distinct sites within the ami locus by southern blotting experiments with a cloned ami+ probe. all prophages have the same restriction map as the d3112 genome extracted from phage particl ... | 1983 | 6318038 |
| [use of deletion mutants of plasmid rp4::d3112 for the genetic analysis of pseudomonas aeruginosa bacteriophage d3112]. | the hybrid plasmid rp4::d3112 becomes unstable in escherichia coli k-12 cells under certain growth conditions. the deletion mutants of this plasmid are formed at a high frequency. all the deletions selected have a specific feature: they start in the left end, at the point of joining of plasmid and phage dna, and remove different portions of the phage genome. the deletion mutants have been used for genetic mapping of d3112. we have localized the repressor gene ci (0-1.3 kb), 3 early genes (1.3-14 ... | 1983 | 6418616 |
| [integration of the genome of the mu-like pseudomonas aeruginosa bacteriophage d3112 into plasmid rp4 and its hybrid plasmid transfer into pseudomonas putida and escherichia coli c600 bacteria]. | the genome of a mu-like bacteriophage d3112 specific for pseudomonas aeruginosa was integrated in vivo into the rp4 plasmid. the fact of integration has been proved by two experiments: 1. the loss of rp4 plasmid is accompanied by loss of d3112 prophage; 2. transfer of the plasmid by conjugation from pseudomonas aeruginosa into bacteria of other species - p. putida pgg1 or escherichia coli c600 leads to the occurrence of clones of these species which liberate phage capable of growing on the lawn ... | 1982 | 6799358 |
| [interaction of pseudomonas aeruginosa plasmids and mu-like phages: the suppression of the early stages of cell infection by phage d3112 in the presence of plasmid rpl11]. | the growth of mu-like, d3112, b39 and b3 bacteriophages of pseudomonas aeruginosa on bacterial strains containing r plasmids was studied. plasmids rpl11, rms148 and rms163 were shown to interfere with phage growth: 1) d3112 and b39 phages do not produce plaques on a lawn of pao1 (rms148) giving e.o.p. less than 10(-9); 2) rpl11 plasmid restricts phage d3112 growth (e.o.p. less than 10(-9), the growth of phage b3 being also restricted by this plasmid, though in considerably less extent; 3) phage ... | 1982 | 6807753 |
| [expression of the genome of mu-like phage d3112 specific for pseudomonas aeruginosa in escherichia coli and pseudomonas putida cells]. | the behavior of escherichia coli cells carrying rp4 plasmid which contains the genome of a mu-like d3112 phage specific for pseudomonas aeruginosa was studied. two different types of d3112 genome expression were revealed in e. coli. the first is bp4-dependent expression. in this case, expression of certain d3112 genes designated as "kil" only takes place when rp4 is present. as a result, cell division stops at 30 degrees c and cells form filaments. cell division is not blocked at 42 degrees c. t ... | 1982 | 6811373 |
| introduction of pseudomonas aeruginosa mutator phage d3112 into alcaligenes eutrophus strain ch34. | we have investigated the possibility of growing mutator phages from pseudomonas aeruginosa on various isolates of alcaligenes eutrophus. although none out of 10 a. eutrophus strains were susceptible to infection with any of the phages tested, phage d3112 could be readily transferred in our model strain ch34 by means of an rp4::d3112 plasmid. ch34/rp4::d3112 lysogens were stable and produced phages. however, neither mitomycin c nor uv treatment increased the phage yield. | 1995 | 7569319 |
| isolation and characterization of mutants defective in the cyanide-insensitive respiratory pathway of pseudomonas aeruginosa. | the branched respiratory chain of pseudomonas aeruginosa contains at least two terminal oxidases which are active under normal physiological conditions. one of these, cytochrome co, is a cytochrome c oxidase which is completely inhibited by concentrations of the respiratory inhibitor potassium cyanide as low as 100 microm. the second oxidase, the cyanide-insensitive oxidase, is resistant to cyanide concentrations in excess of 1 mm as well as to sodium azide. in this work, we describe the isolati ... | 1995 | 7814333 |
| the pilg gene product, required for pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator chey. | the pseudomonas aeruginosa pilg gene, encoding a protein which is involved in pilus production, was cloned by phenotypic complementation of a unique, pilus-defective mutant of strain pao1. this mutant, designated fa2, although resistant to the pilus-specific phage d3112 was sensitive to the pilus-specific phages b3 and f116l. in spite of the unusual phage sensitivity pattern, fa2 lacked the ability to produce functional polar pili (pil) and was incapable of twitching motility (twt). genetic anal ... | 1993 | 8104179 |
| utilization of a mini-dlac transposable element to create an alpha-complementation and regulated expression system for cloning in pseudomonas aeruginosa. | a lac-based alpha-complementation and expression system was developed for use in molecular cloning in pseudomonas aeruginosa. a bacteriophage d3112-based mini-dlac transposable element, containing the laciq-regulated lacz delta m15 gene next to a selectable marker, was constructed. mixed d3112 lysates were used to transduce p. aeruginosa pao1, and derivatives containing randomly inserted chromosomal copies of the mini-dlac element were obtained. transformation of the pao1::mini-dlac transductant ... | 1994 | 8125342 |
| [the phenotype of rpi mutant of pseudomonas aeruginosa phage-transposon d3112 expressed in a heterologous escherichia coli host]. | a clone of escherichia coli ii-16 with unique properties was isolated upon incorporation of hybrid plasmid rp4::d3112 with an integrated genome of phage-transposon d3112 pseudomonas aeruginosa into e. coli c600 cells. the cells of this clone produce viable phage and are not sensitive to growth under low temperatures, which is characteristic of the majority of e. coli (rp4::d3112) clones with the genome of wild type phage. the clone e. coli ii-16 contains phage genome both in an integrated state ... | 1994 | 8188046 |
| [the effect of certain escherichia coli genes on the appearance of the tcs phenotype, conferred by plasmid rp4 with an integrated genome of the d3112 pseudomonas aeruginosa phage]. | the possibility of the sos system activation caused by introduction of a hybrid plasmid rp4::d3112 (where d3112 is a genome of the transposable phage of pseudomonas aeruginosa) into escherichia coli was examined. it has been shown previously that the presence of this plasmid confers to e. coli a so called tcs phenotype: e. coli (rp4::d3112) forms normal colonies and grows at 42 degrees c but does not divide and becomes filamentous at 30 degrees c, probably because of e. coli dna damages generate ... | 1993 | 8405972 |
| characterization of the pseudomonas aeruginosa transposable bacteriophage d3112 a and b genes. | the left end dna of mu-like transposable bacteriophage d3112 was sequenced from bp 2521 to bp 5483. two large open reading frames were identified: orf a (bp 2539-4611) and orf b (bp 4626-5378). orf a can encode a 690 amino acid, 78 kda protein which is 44.4% similar to mu transposase and orf b can encode a 250 amino acid, 27 kda protein, which is 46.4% similar to, though 62 amino acids shorter than, the mu b protein. the cloned d3112 a gene exhibited activity on a mini-d3112-containing plasmid i ... | 1995 | 8547306 |
| [introduction of mutator phage d3112 of pseudomonas aeruginosa into alcaligenes eutrophus var. metallotollerans (strain ch34)]. | it is demonstrated that the intact genome of a d3112 tranposable phage (tp) of pseudomonas aeruginosa, integrated into a recombinant plasmid rp4 :: d3112, can be transferred by means of conjugation from p. putida ppg1 (rp4npt :: d3112) donor cells into alcaligenes eutrophus var. metallotollerans cells. p. aeruginosa strains are unacceptable as donors because they have a bactericidal effect on a. eutrophus. rp4npt :: d3112 plasmid is stably inherited by a. eutrophus with d3112 being expressed and ... | 1996 | 8647419 |
| [interaction between pseudomonas aeruginosa phages-transposons: genetic analysis of the trait of inhibition by prophage d3112 of phage b39 development]. | a novel, previously unknown cip gene of pseudomonas aeruginosa phage d3112 was discovered. the cip gene is responsible for inhibiting the intracellular growth of the related heteroimmune phage b39 of p. aeruginosa. it was shown that the cip gene product inhibits replication of the b39 genome, interacting, apparently, with the definite s+ site in the phage b39 genome. mutants cip- of phage d3112, incapable of inhibiting the growth of phage b39, and b39 mutants, insensitive to the action of the ci ... | 1996 | 8964478 |
| [cloning and study of the expression of pseudomonas aeruginosa cip1 gene by phage-transposon d3112 in a homologous host and in escherichia coli]. | regulatory gene cipl of pseudomonas aeruginosa transposable phage d3112 was cloned, and its expression was studied in p. aeruginosa and escherichia coli. overexpression of the cipl gene prevents transcription and replication of phage d3112 dna and also lysogenization of bacteria p. aeruginosa pao1 by phage d3112. the direction of cipl gene transcription within the vector was determined in the study of cipl gene expression, dependent on its orientation toward the gene lacz promoter. the expressio ... | 1996 | 8974912 |
| [comparison of localizations of insertions of phage-transposons d3112 and b3 into the pseudomonas aeruginosa chromosome]. | preferential chromosomal integration sites (sites of conservative transposition) of transposable phage d3112 were identified by means of pulsed-field gel electrophoresis and separation of restriction fragments of the pseudomonas aeruginosa chromosome. phase b3 was shown to differ from d3112 with respect to this trait. high site-specificity and regional specificity, typical for conservative transposition of d3112, can partially explain the absence of mutations in biosynthetic p. aeruginosa genes ... | 1997 | 9206671 |
| [x811--mutation of the pseudomonas aeruginosa transposable phage d3112 with a pleiotropic effect]. | characteristics of pseudomonas aeruginosa transposable phage d3112 carrying mutation x811 are described. x811 is a recessive mutation with pleiotropic effect. it determines a deteriorated lysis of infected or induced bacteria, a delayed replication, and a considerably decreased replication rate. in addition, the x811 mutation is expressed as the kil phenotype, since high-temperature induction of prophage d3112 cts15 x811 does not cause an immediate decrease in the ability of bacteria to form col ... | 1999 | 10368781 |
| characterization of the lysogenic repressor (c) gene of the pseudomonas aeruginosa transposable bacteriophage d3112. | bacteriophage d3112 is a mu-like temperate transposable phage of pseudomonas aeruginosa. genetic mapping and dna sequence analysis have identified the left end of the phage genome as encoding the transposase enzyme (a) and the lysogenic (c) repressor. the c open reading frame (orf), located at the leftmost end of the phage genome and transcribed from right to left, has four possible gtg initiation codons. using site-directed mutagenesis, each of the four gtg codons was modified to gta, which can ... | 2000 | 10873751 |
| complete sequence and evolutionary genomic analysis of the pseudomonas aeruginosa transposable bacteriophage d3112. | bacteriophage d3112 represents one of two distinct groups of transposable phage found in the clinically relevant, opportunistic pathogen pseudomonas aeruginosa. to further our understanding of transposable phage in p. aeruginosa, we have sequenced the complete genome of d3112. the genome is 37,611 bp, with an overall g+c content of 65%. we have identified 53 potential open reading frames, including three genes (the c repressor gene and early genes a and b) that have been previously characterized ... | 2004 | 14702309 |
| complete genomic sequence of bacteriophage b3, a mu-like phage of pseudomonas aeruginosa. | bacteriophage b3 is a transposable phage of pseudomonas aeruginosa. in this report, we present the complete dna sequence and annotation of the b3 genome. dna sequence analysis revealed that the b3 genome is 38,439 bp long with a g+c content of 63.3%. the genome contains 59 proposed open reading frames (orfs) organized into at least three operons. of these orfs, the predicted proteins from 41 orfs (68%) display significant similarity to other phage or bacterial proteins. many of the predicted b3 ... | 2004 | 15375138 |