| further biological and molecular characterization of actinophage vwb. | the development cycle of the temperate actinophage vwb was investigated. adsorption of most phage particles occurred within 30 min and the adsorption constant was 0.6 x 10(-8) ml min-1. the latent and rise periods were 140 and 100 min, respectively, and the burst size was estimated to be 130-250 p.f.u. although phage vwb could infect only streptomyces venezuelae eth 14630 (atcc 40755), of six different s. venezuelae strains tested, phage dna could be introduced by transfection into most non-infe ... | 1990 | 2230721 |
| determination of the packaging capacity of bacteriophage vwb. | vwb is a temperate bacteriophage whose chromosome has cohesive ends. vwb can stably package modified chromosomes that contain insertions of up to about 4 kilobases of foreign dna. phage particles containing extra dna differ from the wild type in their increased sensitivity to chelating agents. because of these properties, vwb is a promising cloning vector for streptomyces venezuelae. | 1990 | 2298695 |
| a restriction endonuclease map of streptomyces phage vwb. | a physical map of the actinophage vwb has been constructed using the restriction endonucleases bglii, clai, ecori, ecorv, hindiii, kpni and sphi. phage vwb, genome size 47.3 kb, propagates on streptomyces venezuelae, and it can also lysogenise this species. the three bglii-generated fragments of vwb dna were cloned in pbr322, and subsequently mapped. in this manner the restriction map of the vwb phage genome was constructed. | 1985 | 2995769 |
| analysis of the open reading frames of the main capsid proteins of actinophage vwb. | the nucleotide sequence of a 6 kb fragment encoding the main late proteins (p14, p38 and p24) of actinophage vwb was obtained. sequence comparison of the encoded proteins with those filed in databases indicated that the phage vwb main late proteins were all novel. a search for special motifs revealed that p14 (13.3 kda) has a p-loop sequence commonly found in atp- and gtp-binding proteins. this observation might indicate that p14 is important for atp-driven dna translocation during encapsidation ... | 1995 | 7611876 |
| site-specific integration of bacteriophage vwb genome into streptomyces venezuelae and construction of a vwb-based integrative vector. | the temperate bacteriophage vwb integrates into the chromosome of streptomyces venezuelae eth14630 via site-specific integration. following recombination of the vwb attp region with the chromosomal attb sequence, the host-phage junctions attl and attr are formed. nucleotide sequence analysis of attp, attb, attl and attr revealed a 45 bp common core sequence. in attb this 45 bp sequence consists of the 3' end of a putative trna arg(agg) gene with a 3'-terminal cca sequence which is typical for pr ... | 1998 | 9884227 |
| complete genomic nucleotide sequence and analysis of the temperate bacteriophage vwb. | the entire double-stranded dna genome of the streptomyces venezuelae bacteriophage vwb was sequenced and analyzed. its size is 49,220 bp with an overall molar g + c content of 71.2 mol%. sixty-one potential open reading frames were identified and annotated using several complementary bioinformatics tools. clusters of functionally related putative genes were defined, supporting a refined version of the modular theory of phage evolution. | 2005 | 15629775 |