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general method for the isolation of conditional lethal mutants in any required region of the virus genome: its application to the 'semi-essential' region of phage mu.a general method is presented that enables viral mutants to be isolated in any required region of their genome. an application of the method is reported relating to the isolation of conditional lethal mutants in the "semi-essential' region of phage mu. some properties are described of one mutant found in this way, mucts62seeam8 apl, which is typical of a class of mutants that were isolated.19862940329
chromosomal integration mechanism of infecting mu virion dna.dna transposition is central to the propagation of temperate phage mu. a long-standing problem in mu biology has been the mechanism by which the linear genome of an infecting phage, which is linked at both ends to dna acquired from a previous host, integrates into the new host chromosome. if mu were to use its well-established cointegrate mechanism for integration (single-strand nicks at mu ends, joined to a staggered double-strand break in the target), the flanking host sequences would remain l ...200616484193
microarray analysis of transposition targets in escherichia coli: the impact of transcription.transposable elements have influenced the genetic and physical composition of all modern organisms. defining how different transposons select target sites is critical for understanding the biochemical mechanism of this type of recombination and the impact of mobile genes on chromosome structure and function. phage mu replicates in gram-negative bacteria using an extremely efficient transposition reaction. replicated copies are excised from the chromosome and packaged into virus particles. each v ...200415210965
functional genomics on potato virus a: virus genome-wide map of sites essential for virus propagation.transposition-based in vitro insertional mutagenesis strategies provide promising new approaches for functional characterization of any cloned gene or genome region. we have extended the methodology and scope of such analysis to a complete viral genome. to map genome regions both essential and nonessential for potato virus a propagation, we generated a genomic 15-bp insertion mutant library utilizing the efficient in vitro dna transposition reaction of phage mu. we then determined the proficienc ...200211932242
a large nucleoprotein assembly at the ends of the viral dna mediates retroviral dna integration.we have probed the nucleoprotein organization of moloney murine leukemia virus (mlv) pre-integration complexes using a novel footprinting technique that utilizes a simplified in vitro phage mu transposition system. we find that several hundred base pairs at each end of the viral dna are organized in a large nucleoprotein complex, which we call the intasome. this structure is not formed when pre-integration complexes are made by infecting cells with integrase-minus virus, demonstrating a requirem ...19979405379
supercoiling and integration host factor change the dna conformation and alter the flow of convergent transcription in phage mu.transcription of bacteriophage mu is modulated by its repressor, by negative supercoiling, and by the escherichia coli protein integration host factor (ihf). two converging mu promoters regulate lytic and lysogenic development. the influence of ihf on these convergent promoters depended on the dna conformation. when mu operator dna changed from the relaxed to the negative supercoiled form, ihf changed from a stimulatory factor to an inhibitor of the repressor promoter, and the ratio of the lytic ...19892644278
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