| human papillomavirus type 58 dna sequence. | the complete nucleotide sequence of human papillomavirus type 58 (hpv 58) dna cloned from an invasive cervical carcinoma was determined. the hpv 58 genome consists of 7824 nucleotides, containing 37.9% of gc residues, and has a similar genome organization of other hpvs. on the nucleotide sequence level, it conserves the signal sequences for regulation of gene expression as with other genital hpvs and exhibits an extensive homology with hpv 33 (77%). comparative analysis of amino acid sequences r ... | 1991 | 1656594 |
| genotyping of 22 human papillomavirus types by dna chip in korean women: comparison with cytologic diagnosis. | more sensitive and reliable methods than individual testing (such as polymerase chain reaction, restriction fragment length polymorphism, and southern blot) should be developed as screening tools for the detection of latent human papillomavirus. today, the new bethesda system recommends human papillomavirus testing as an adjuvant to the conventional papanicolaou smear for more comprehensive identification of women at certain risk of cervical neoplasia. we performed human papillomavirus genotypin ... | 2003 | 12548196 |
| high prevalence of human papillomavirus type 58 in mexican colposcopy patients. | cervical cancer is the second most common cancer of the women worldwide. infection with some genotypes of human papillomavirus is the most important risk factor associated to cervical cancer. | 2004 | 14962790 |
| distribution of human papillomavirus type 58 variants in progression of cervical dysplasia in korean women. | this cross-sectional study examined the distribution of hpv 58 sequence variation in korean women for the first time. among 1,750 korean women, 53 women were positive for hpv 58 single infection, of whom 26 were without disease, 20 were with cervical intraepithelial neoplasia (cin) 1, and 7 with cin 2 or 3. altogether, 36 different nucleotide sequence variations were identified with the l1, 20 within e2, 5 within e6, and 10 within e7. further studies on variants of oncogenic hpvs are necessary, ... | 2009 | 19809265 |
| purification and immunogenicity study of human papillomavirus 58 virus-like particles expressed in pichia pastoris. | two human papillomavirus (hpv) prophylactic vaccines are currently available in the market: gardasil and cervarix. these two vaccines work against tumor high-risk subtypes hpv 16 and hpv 18. however, they do not include other high-risk subtypes such as hpv 58. epidemiological research in china shows that hpv 58 is a prevalent high-risk subtype, second only to hpv 16 and hpv 18. thus, for cervical cancer prevention in china, developing a vaccine against hpv 58 is necessary. in this study, hpv 58 ... | 2011 | 21821128 |
| the preparation of human papillomavirus type 58 vaccine and exploring its biological activity and immunogenicity in vitro. | : human papillomavirus (hpv) type 58 is the second most prevalent virus infection among chinese women. to develop an hpv58 vaccine that combines both prophylactic and therapeutic functions, we generate a chimeric virus-like particle (cvlp). | 2011 | 21738041 |
| identification of human papillomavirus type 58 lineages and the distribution worldwide. | background. human papillomavirus type 58 (hpv-58) accounts for a much higher proportion of cervical cancers in east asia than other types. a classification system of hpv-58, which is essential for molecular epidemiological study, is lacking. methods and results. this study analyzed the sequences of 401 isolates collected from 15 countries and cities. the 268 unique concatenated e6-e7-e2-e5-l1-lcr sequences that comprised 57% of the whole hpv-58 genome showed 4 distinct clusters. l1 and lcr produ ... | 2011 | 21592985 |
| replication and transcription of human papillomavirus type 58 genome in saccharomyces cerevisiae. | to establish a convenient system for the study of human papillomavirus (hpv), we inserted a saccharomyces cerevisiae selectable marker, ura, into hpv58 genome and transformed it into yeast. | 2010 | 21156081 |
| simple and convenient chromatography-based methods for purifying the pseudovirus of human papillomavirus type 58. | the currently available purification protocol for human papillomavirus (hpv) pseudovirus (psv), as a substitute for the native hpv virion, utilizes optiprep gradients (og), which require costly equipment such as ultracentrifuges, and 4-7 h of working time, and cannot cope with large psv samples. to develop a convenient method for purifying hpv type 58 psv (hpv58 psv) we have examined the use of heparin chromatography (hc) and cation-exchange chromatography (cc), which utilize open column systems ... | 2010 | 21059392 |
| distribution of human papillomavirus 58 and 52 e6/e7 variants in cervical neoplasia in chinese women. | the specific intratype hpv genome variations may be associated with the development of cervical cancer in specific geographic regions and a given population. human papillomavirus (hpv) 58 and 52 have been found to be relatively prevalent among asian women including chinese women. this study aimed to assess the risk of hpv 58 and 52 variants for cervical cancer and its precursors in chinese women. | 2010 | 20870281 |
| t-cell response to human papillomavirus type 58 l1, e6, and e7 peptides in women with cleared infection, cervical intraepithelial neoplasia, or invasive cancer. | human papillomavirus type 58 (hpv-58) exists in a relatively high prevalence in certain parts of the world, including east asia. this study examined the t-cell response to hpv-58 l1, e6, and e7 peptides among women with cleared infection, cervical intraepithelial neoplasia grade 2 (cin2) or cin3, or invasive cervical cancer (icc). peptides found to be reactive in the in vitro peptide binding assay or mouse-stimulating study were tested with a gamma interferon (ifn-gamma) enzyme-linked immunospot ... | 2010 | 20668141 |
| high prevalence and low e6 genetic variability of human papillomavirus 58 in women with cervical cancer and precursor lesions in southeast mexico. | infection with some genotypes of human papillomavirus (hpv) is the most important risk factor associated with cervical cancer (cc). throughout the world, hpv type 58 prevalence varies from one region to another; it is higher in women from certain countries in asia and latin america, such as china and mexico. although intratypic variants have been reported on a few occasions, our knowledge about hpv 58 genetic variation remains limited. therefore, this work aims to (i) determine the prevalence of ... | 2010 | 20428672 |
| binding and neutralization characteristics of a panel of monoclonal antibodies to human papillomavirus 58. | human papillomavirus (hpv) 58 is a high-risk hpv type associated with progression to invasive genital carcinomas. we developed six monoclonal antibodies (mabs) against hpv58 l1 virus-like particles that bind conformational epitopes on hpv58. the hybridoma cell lines were adapted to serum- and animal component-free conditions and the mab supernatants were affinity-purified. the six mabs neutralized hpv58 pseudoviruses (psvs) and 'quasivirions' with different capacities. the mabs differed in their ... | 2010 | 20181746 |
| phylogeographic analysis of human papillomavirus 58. | human papillomavirus 58 (hpv58) is one type of hpv with high risk of causing cervical cancer. unusually high prevalence of hpv58 has been reported in asia, africa and some other areas. however, due to the scattered distribution of global data, in addition to the lack of data of some hpv58 high-incidence nations and regions, like mainland china, a comprehensive analysis of the global geographical distribution of hpv58 remains blank so far. in this study, hpv58 from the human cervical cancer tissu ... | 2009 | 20016974 |
| the human papillomavirus type 58 e7 oncoprotein modulates cell cycle regulatory proteins and abrogates cell cycle checkpoints. | hpv type 58 (hpv-58) is the third most common hpv type in cervical cancer from eastern asia, yet little is known about how it promotes carcinogenesis. in this study, we demonstrate that hpv-58 e7 significantly promoted the proliferation and extended the lifespan of primary human keratinocytes (phks). hpv-58 e7 abrogated the g1 and the postmitotic checkpoints, although less efficiently than hpv-16 e7. consistent with these observations, hpv-58 e7 down-regulated the cellular tumor suppressor prb t ... | 2010 | 19945133 |
| the prevalence of human papillomavirus type 58 in chinese patients with cervical carcinoma and its influence on survival. | to assess the prevalence of human papillomavirus (hpv) type-specific infections and its influence on prognosis and survival rate of cervical cancer patients treated with radiotherapy alone or in combination with chemotherapy or hyperthermotherapy or both. | 2009 | 19875274 |
| high prevalence of human papillomavirus type 58 in patients with cervical pre-malignant lesions in southern brazil. | cervical cancer is the second most common type of cancer in women worldwide. several human papillomavirus (hpv) genotypes, sexual behavior, and socioeconomic profile represent major risk factors for the development of this carcinoma. cervical invasive cancer is preceded by cellular abnormalities that can be identified by cytological or histological exams. in order to determine the prevalence and genotypes of hpv in women with abnormal cytology or histopathology, cervical cell samples from 256 pa ... | 2009 | 19475616 |
| human papillomavirus-58 and -73-associated digital squamous cell carcinoma in a patient with aggressive digital papillary adenocarcinoma. | aggressive digital papillary adenocarcinoma (adpa) is a rare tumor that is considered to arise from eccrine sweat glands of the skin. it occurs predominantly in men with a mean age in the sixth decade. it shows a strong tendency for local recurrence and has the potential to metastasize to distant sites. prompt diagnosis and regular follow-up are important to ensure the best possible outcome. we discuss a case of recurrent adpa associated with subsequent squamous cell carcinoma (scc) in different ... | 2009 | 19461243 |
| human papillomavirus 58 in a squamous cell carcinoma of the tongue. | | 2009 | 19362048 |
| profile of physical status and gene variation of human papillomavirus 58 genome in cervical cancer. | epidemiological studies have shown that human papillomavirus 58 (hpv 58) is found at a relatively high frequency in east asia and some regions of central and south america. to investigate the physical status of hpv 58 and analyse sequence variations of hpv 58 in cervical cancer patients, the hpv 58 genome in 37 hpv 58-positive cervical cancer specimens collected from china were investigated by a mapping analysis based on nested pcr and nucleotide sequencing. a pure integrated genome was found in ... | 2009 | 19264634 |
| an optimized yeast cell-free system: sufficient for translation of human papillomavirus 58 l1 mrna and assembly of virus-like particles. | we developed a yeast cell-free system suitable for in vitro translation of human papillomavirus 58 (hpv58) l1 mrna. this system was systematically optimized resulting in enhanced translation efficiency. the optimal concentrations of potassium and magnesium ions observed were specific to the hpv58 l1 protein production. supplementation with sucrose in the preparation of the yeast lysate greatly enhanced its stability. after optimization, protein production in this system was significantly superio ... | 2008 | 18691524 |
| [the preliminary analysis on immunogenicity of dna vaccines against human papillomavirus 58]. | in order to evaluate the immunogenicity of hpv 58 l1 dna vaccines, five dna vaccines had been constructed with pcdna3.1 vector containing different l1 genes of hpv 58, which were designated as l1h, l1hdeltac, l1s, l1sm and l1wt. the protein expression of dna vaccines in vitro was tested by western blot. the ability of forming pseudovirus was evaluated by transfecting dna vaccine together with pcdna3.1-h58l2 and pcdna3.1-gfp into 293ft cells. the neutralizing antibodies and cellular immune respon ... | 2008 | 18683556 |
| carboxyl terminus truncated human papillomavirus type 58 l1 protein maintains its bioactivity and ability to form virus-like particles. | to prepare carboxyl terminus truncated human papillomavirus type 58 l1 (hpv58l1) protein and evaluate its ability to form virus-like particles, the baculovirus and sf-9 insect cells was used to express hpv58l1 protein, and pfastbac-htb containing hpv58l1 gene sequence of carboxyl terminus truncation was generated. then sf-9 cells were infected with recombinant baculovirus. after being cultured, the post-infected cells expressing--hpv58l1 protein were harvested and analyzed by sds-page and wester ... | 2004 | 15791833 |
| l1 sequence of a new human papillomavirus type-58 variant associated with cervical intraepithelial neoplasia. | the present study on molecular characterization of a human papillomavirus (hpv) isolated in central brazil describes the l1 gene sequence from a new variant of hpv-58, the isolate bsb-02. the sample was from a smear obtained from a woman with cervical intraepithelial neoplasia grade ii. the whole l1 gene from isolate bsb-02 was sequenced automatically, showing 99.1% nucleotide identity with the gene from the hpv-58 reference. the clustering between bsb-02 and hpv-58 reference sequence was also s ... | 2005 | 15665981 |
| detection of human papillomavirus type 58 in polydactylous bowen's disease on the fingers and toes of a woman - concurrent occurrence of invasive vulval and cervical carcinomas. | the group related to human papillomavirus (hpv) type 16 (hpv-16, -31, -33, -35, -52, -58 and -67) is dominantly identified in cervical intraepithelial neoplasia and cervical carcinomas. hpv-16 has also been frequently detected in bowen's disease on the hands and feet. we describe herein a case of polydactylous bowen's disease on the fingers and toes of a woman who had had radical vulvectomy and hysterectomy for concomitant invasive vulval and cervical carcinomas. all the lesions, except for the ... | 2004 | 15459536 |
| association of human papillomavirus type 58 variant with the risk of cervical cancer. | human papillomavirus (hpv) type 58 has been found to be prevalent among chinese patients with cervical cancer. this study examined the oncogenic risk of hpv58 variants in hong kong, a southern part of china. altogether, 1924 women were studied: 42.8% with a normal cervix, 16.2% with cervical intraepithelial neoplasia (cin) i, 12.7% with cin ii, 20.8% with cin iii, and 7.6% with invasive cervical cancer (icc). the overall prevalence of hpv58 was 11.4% (220) and increased statistically significant ... | 2002 | 12189229 |
| [expression of the human papillomavirus type 58l1 capsid protein in e.coli and preparation of the hpv58 l1 mouse antiserum]. | human papillomavirus type 58 (hpv58) has been shown to be one of the most important highly-oncogenic, risky hpv types. the present study aimed at investigating the expression of the hpv58 l1 major capsid protein by e.coli and preparation of hpv58 l1 antiserum. | 2001 | 11986714 |
| human papillomavirus type 58 in bowen's disease of the elbow. | human papillomavirus (hpv) can be detected in skin lesions of bowen's disease, particularly on the fingers, and its genotype is associated with mucosal/genital types of hpv. we report herein an 85-year-old woman who had hpv-associated bowen's disease on her elbow. hpv-58 dna was detected in the lesion by polymerase chain reaction with restriction fragment length polymorphism and by southern blot hybridization. in situ hybridization revealed numerous hybrid cells in the nuclei of the upper epider ... | 2001 | 11251579 |
| high prevalence of human papillomavirus type 58 in chinese women with cervical cancer and precancerous lesions. | the prevalence of human papillomavirus (hpv) among 332 hong kong chinese women with abnormal papanicolaou smears were determined by polymerase chain reaction and restriction fragment length polymorphism analysis. the overall hpv positive rate was 44.3% with 18.6% (16/86) for normal/inflamed cervices, 36.4% (32/88) for condyloma, 64.7% (33/51) for cervical intraepithelial neoplasia grade 1 (cin 1), 37.9% (11/29) for cin 2, 68.3 (41/60) for cin 3, and 77.8% (14/18) for carcinoma. double hpv infect ... | 1999 | 10459162 |
| epitomics: igg-epitome decoding of e6, e7 and l1 proteins from oncogenic human papillomavirus type 58. | to enable rational multi-epitope vaccine and diagnostic antigen design, it is imperative to delineate complete igg-epitome of the protein. here, we describe results of igg-epitome decoding of three proteins from high-risk (hr-) oncogenic human papillomavirus type 58 (hpv58). to reveal their entire epitomes, employing peptide biosynthetic approach, 30 precise linear b-cell epitopes (bces) were mapped on e6, e7 and l1 proteins using rabbits antisera to the respective recombinant proteins. using se ... | 2016 | 27708433 |
| human papillomavirus genotype distribution in cervical intraepithelial neoplasia grade 2/3 and invasive cervical cancer in japanese women. | human papillomavirus vaccines are being introduced worldwide and are expected to reduce the incidence of cervical cancer. here we report a cross-sectional study using a validated human papillomavirus genotyping method to reveal the human papillomavirus prevalence and genotype distribution in japanese women with cervical intraepithelial neoplasia grade 2/3 and invasive cervical cancer. | 2014 | 25156680 |
| a dna vaccine encoding mutated hpv58 me6e7-fc-gpi fusion antigen and gm-csf and b7.1. | persistent infection with high-risk human papillomavirus (hpv) is a predominant cause of cervical cancer, and hpv58 is the third most common virus detected in the patients with cervical cancer in asia. e6 and e7 are the viral oncogenes which are constitutively expressed in hpv-associated tumor cells and can be used as target antigens for related immunotherapy. in this study, we modified the hpv58 e6 and e7 oncogenes to eliminate their oncogenic potential and constructed a recombinant dna vaccine ... | 2015 | 26604780 |
| association of human papillomavirus type 58 with breast cancer in shaanxi province of china. | a number of reports have identified hpv dna in breast cancer specimens and hpv type 16, 18, 31, 33, 45, and 51 were more prevalent. hpv 58 was frequently detected in cervical cancer in shaanxi china. the aim of the present study was to investigate whether hpv 58 present in breast cancer. 169 cases of breast cancer samples and 83 benign breast lesions were analyzed. type specific primers and oligonucliotide probe were used for the detection of hpv 58 by conventional pcr and in situ hybridization ... | 2015 | 25676062 |
| [construction and eukaryotic expression of pvax1-hpv58me6e7fcgb composite gene vaccine]. | to construct and express a composite gene vaccine for human papillomavirus 58(hpv58)-associated cervical cancer, we inserted hpv58me6e7 fusion gene into pci-fc-gpi eukaryotic expression vector, constructing a recombinant plasmid named pci-sig-hpv58me6e7-fc-gpi. then we further inserted fragment of sig-hpv58me6e7fc-gpi into the novel vaccine vector pvax1-ires-gm/b7, constructing pvax1-hpv58me6e7fcgb composite gene vaccine. pvax1-hpv58me6e7fcgb vaccine was successfully constructed and identified b ... | 2013 | 24459978 |
| genome sequencing accuracy by rca-seq versus long pcr template cloning and sequencing in identification of human papillomavirus type 58. | genome variations in human papillomaviruses (hpvs) are common and have been widely investigated in the past two decades. hpv genotyping depends on the finding of the viral genome variations in the l1 orf. other parts of the viral genome variations have also been implicated as a possible genetic factor in viral pathogenesis and/or oncogenicity. | 2014 | 24410913 |
| an optimized yeast cell-free lysate system for in vitro translation of human virus mrna. | yeast (saccharomyces cerevisiae) as a model organism has long been established to study various aspects of eukaryotic cellular and molecular biology. cell-free lysates prepared from different yeast strains have been used as a powerful tool to study eukaryotic protein expression in vitro. recently, we established a yeast cell-free lysate system for in vitro translation long and short l1 capsid gene mrnas of human papillomavirus type 58. we were able to significantly improve the translation effici ... | 2014 | 24395419 |
| prevalence of human papillomavirus type 58 in women with or without cervical lesions in northeast brazil. | human papillomavirus (hpv) has been recognized as a necessary, but not sufficient, cause of cervical cancer. | 2013 | 24379999 |
| designing of epitope-focused vaccine by targeting e6 and e7 conserved protein sequences: an immuno-informatics approach in human papillomavirus 58 isolates. | human papillomavirus (hpv) is a dna virus that belongs to the papillomavirus family and is capable of infecting humans. currently, few vaccines are available to prevent infection by hpv. however, they are not so much effective and provide little benefit to women who have already been infected with hpv. the aim of this study was to design epitope-based vaccines of hpv58 by targeting e6 and e7 proteins of hpv58. proteomic sequences were retrieved from different isolates at different time periods a ... | 2016 | 27640170 |
| high prevalence of human papillomavirus type 58 in hiv infected men who have sex with men: a preliminary report in central italy. | human papillomavirus (hpv) infection and type-specific prevalence at anal, oral, coronal sulcus, and urethral mucosa in fifty hiv positive men having sex with men (msm) were evaluated; patients were enrolled in a non-metropolitan area of central italy. clinical and socio-demographic information, drug, and sexual behaviors were obtained for each participant. hpv was detected by pcr from an overall of 200 specimens, and genotyping was performed by both restriction fragment length polymorphism anal ... | 2016 | 26467111 |
| [transformation activity and antigenicity of the human papillomavirus type 58 e6e7 fusion gene mutant]. | to develop a prophylactic and therapeutic vaccine against human papillomavirus (hpv) type 58-associated cervical carcinoma, and explore its transformation activity and antigenicity. | 2013 | 24257298 |
| evidence of the causal role of human papillomavirus type 58 in an oropharyngeal carcinoma. | persistent human papillomavirus infection (hpv) is recognized as an important etiologic factor for a subset of head and neck squamous cell carcinomas (scc), especially those arising from the oropharynx. whereas hpv16 accounts for the majority of hpv dna-positive oropharyngeal scc, infections with other mucosal high-risk hpv types are quite rare and biological data demonstrating their causal involvement are insufficient. here we present the first case of an oropharyngeal scc driven by hpv type 58 ... | 2013 | 24220072 |
| methylation of human papillomavirus-52 and -58 is a candidate biomarker in cervical neoplasia. | previous studies of human papillomavirus (hpv)16/18 genome methylation have concluded that methylation status of the l1 gene might act as a biomarker for cervical intraepithelial neoplasia (cin). | 2013 | 23871162 |
| human papillomavirus type 58 genome variations and rna expression in cervical lesions. | human papillomavirus type 58 (hpv58) is relatively prevalent in china and other asian countries. in this study, the hpv58 genome in cervical lesions was decoded from five grade 2 or 3 cervical intraepithelial neoplasia lesion (cin2/3) samples and five cervical cancer tissues using rolling-circle amplification of total cell dna and deep sequencing and verified by whole-genome cloning and sequencing. hpv58 isolates from china feature a total of 52 nucleotide substitutions (0.66%) from the referenc ... | 2013 | 23785208 |
| detection of human papillomavirus type 58 in periungual bowen's disease. | | 2013 | 23529176 |
| detection of the human papillomavirus 58 physical state using the amplification of papillomavirus oncogene transcripts assay. | hpv 58 is detected commonly in cervical cancer in east asian countries. to evaluate the hpv 58 physical state, the amplification of papillomavirus oncogene transcripts (apot) and hybridisation assays were established. episome- and integrate-derived transcripts were confirmed by direct sequencing. twenty-nine hpv 58 positive samples from various cervical lesions were used. the results showed that the episome-derived transcripts were recognised as two major specific amplified products (1040 and 71 ... | 2013 | 23466631 |
| variations of human papillomavirus type 58 e6, e7, l1 genes and long control region in strains from women with cervical lesions in liaoning province, china. | infection with certain types of human papillomaviruses (hpvs) is a risk factor for the development of cervical cancer. hpv type 58 (hpv 58) is prevalent among chinese women. the intratype sequence variants differ in oncogenic potential and their prevalences vary across geographic regions. the objective of this study was to analyze the variations of hpv 58 e6, e7, l1 genes and long control region (lcr) in a large samples collected from northeastern chinese women with cervical lesions. a total of ... | 2012 | 22659102 |
| human papillomavirus type 58: the unique role in cervical cancers in east asia. | about 15 types of human papillomavirus (hpv) are classified as high-risk based on their epidemiological link with cervical cancer. these hpv types have deferent degrees of oncogenicity and their distribution among cervical precancers and cancers varies ethnogeographically. hpv58 is rare worldwide but being found more commonly in east asia. | 2012 | 22571619 |
| type-specific interaction between human papillomavirus type 58 e2 protein and e7 protein inhibits e7-mediated oncogenicity. | human papillomavirus type 58 (hpv-58) is a very common hpv type in eastern asia. little is known about its biology and tumorigenesis. in this study, hpv-58 e2 protein (58e2) was found to interact with e7 protein (58e7), and the hinge domain of 58e2 was shown to be responsible for binding to the 58e7 protein. interestingly, the e2-e7 interaction appears to be hpv type-specific, as we found that the hpv-16 e2 could not bind to the 58e7 protein, and neither did 58e2 interact with hpv-16 e7. the bio ... | 2012 | 22442110 |
| codon optimization of the human papillomavirus type 58 l1 gene enhances the expression of soluble l1 protein in saccharomyces cerevisiae. | the effect of codon optimization of l1 gene on the production of the l1 protein of human papillomavirus (hpv) was investigated in a yeast expression system. saccharomyces cerevisiae was transformed with a plasmid containing either the wild type (ws)-hpv type 58 l1 (hpv58 l1) gene or a codon-optimized (mo)-hpv58 l1 gene. the proportion of soluble l1 protein expressed from mo-hpv58 l1 was significantly higher than that expressed from ws-hpv58 l1. moreover, the amount of purified mo-hpv58 l1 protei ... | 2013 | 23183918 |
| geographical distribution and oncogenic risk association of human papillomavirus type 58 e6 and e7 sequence variations. | human papillomavirus (hpv) 58 accounts for a notable proportion of cervical cancers in east asia and parts of latin america, but it is uncommon elsewhere. the reason for such ethnogeographical predilection is unknown. in our study, nucleotide sequences of e6 and e7 genes of 401 hpv58 isolates collected from 15 countries/cities across four continents were examined. phylogenetic relationship, geographical distribution and risk association of nucleotide sequence variations were analyzed. we found t ... | 2013 | 23136059 |
| the production and immunogenicity of human papillomavirus type 58 virus-like particles produced in saccharomyces cerevisiae. | human papillomavirus (hpv) is the cause of most cases of cervical cancer. hpv type 58 (hpv58) is the second most frequent cause of cervical cancer and high-grade squamous intraepithelial lesions (hsil) in asia and south / central america, respectively. however, there is no vaccine against hpv58, although there are commercially available vaccines against hpv16 and 18. in this study, we produced hpv58 l1 protein from saccharomyces cerevisiae, and investigated its immunogenicity. we first determine ... | 2012 | 23124750 |
| human papillomavirus type 58 l1 virus-like particles purified by two-step chromatography elicit high levels of long-lasting neutralizing antibodies. | human papillomavirus (hpv) type 58 is a high-risk type of hpv frequently detected in cervical cancers, especially in eastern asia. there are still no commercially available vaccines against hpv 58 infection. high levels of long-lasting neutralizing antibodies are crucial for long-term protection against hpv infection. here, we have developed a two-step chromatography strategy and have purified highly pure hpv l1 proteins, which form more homogenous and uniform vlps than those purified by cscl ul ... | 2013 | 22965579 |
| the development of dna-based quartz crystal microbalance integrated with isothermal dna amplification system for human papillomavirus type 58 detection. | to address the effect of dramatic change in temperature and viscosity during pcr process on quartz crystal microbalance (qcm) sensor and to increase the sensitivity, isothermal amplification was employed in the system. we combined loop-mediated isothermal amplification (lamp) technique with qcm, called as lamp-qcm, for detection of high-risk human papillomavirus viral dna type 58 (hpv-58) which is commonly found in asian women. the liquid-phase lamp-qcm prototype comprised the frequency counter, ... | 2013 | 22959017 |