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identification of an immunologically distinct papillomavirus from lesions of epidermodysplasia verruciformis.virions isolated from warts of 2 siblings with epidermodysplasia verruciformis (ev), a rare disease characterized by the lifelong growth of warty skin tumors containing papova-like virions, were compared to isolates of human papillomavirus (hpv) from 3 pools of plantar and common hand warts. the length of relaxed, circular (form ii) molecules of ev virion dna approximated the length of hpv dna but was 3.3% longer. antisera prepared in rabbits against the 3 hpv pools coated and aggregated hpv in ...197771354
primary tissue culture of human wart-derived epidermal cells (keratinocytes).the formation of human skin warts may depend on transformation by human papilloma virus or result from a productive virus infection. to explore this problem, methods were developed for the primary tissue culture of epidermal cells (keratinocytes) derived from wart tissues and normal skin. wart-derived keratinocytes continued to proliferate for periods exceeding 3 months, but the patterns of cell growth were identical to normal keratinocytes. electron microscopic and immunohistologic examination ...1975164558
structural polypeptides of rabbit, bovine, and human papillomaviruses.the number and apparent molecular weight of the structural polypeptides of shope rabbit papilloma virus (rpv), bovine papilloma virus (bpv), and human papilloma virus (hpv) were estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. up to 10 polypeptides were detected in highly purified bpv and hpv full particles; a close homology was found between the polypeptide composition of both viruses. purified rpv virions gave a similar polypeptide pattern. the main components of the thr ...1975167184
persistence of viral dna in human cell cultures infected with human papilloma virus. 1975167292
human papillomavirus dna: physical map.human papillomavirus (hpv) dna form i (supercoiled) was prepared from plantar warts. hpv dna was cleaved with restriction enzymes obtained from the following sources: escherichia coli (ecori), hemophilus influenzae strain rd (both unfractionated hind and aeparated hindii and hindiii enzymes) and hemophilus parainfluenzae (hpai). the cleavage products were analyzed by polyacrylamide gradient slab gel electrophoresis and electron microscopy. hpv dna was cleaved into two fragments by ecori (87% and ...1975174077
human papilloma virus dna: physical mapping and genetic heterogeneity.the molecular weight of three preparations of human papilloma virus dna derived from different plantar warts was determined by agarose gel electrophoresis or electron microscopic contour length measurement. it was found to amount to approximately 4.9 x 10(6). analysis of this dna after sequential digestion by four different restriction endonucleases (ecori, bam, hind ii, and hind iii) permitted physical mapping of the cleavage sites. two of the three dna preparations revealed an identical cleava ...1976177985
[human papilloma virus (author's transl)]. 1976189105
chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses.four low-molecular-weight polypeptides migrating like h2a, h2b, h3, and h4 calf liver histones were detected by sodium dodecyl sulfate-acrylamide gel electrophoresis of highly purified preparations of bovine papillomavirus (bpv) and human papillomavirus (hpv). complexes of these polypeptides and viral dna were isolated by agarose-gel filtration of the alkaline disruption products of both viruses. when observed under the electron microscope, these complexes appeared as circular structures compose ...1977191643
human papillomavirus dna: physical mapping of the cleavage sites of bacillus amyloliquefaciens (bami) and haemophilus parainfluenzae (hpaii) endonucleases and evidence for partial heterogeneity.the dna of human papillomavirus (hpv) obtained from a pool of plantar warts is cleaved by bacillus amyloliquefaciens (bami) and haemophilus parainfluenzae (hpaii) restriction endonucleases at one and four specific sites, respectively. these sites were localized on the previously established cleavage map of hpv dna, using the hind, hindiii, hpai, and ecori endonuclease restriction sites as reference. the four hpaii sites were mapped, clockwise, at 1.4, 41.1, 44.3, and 52.8% of the genome length f ...1977191644
in vitro-labeled dna for detecting viral genomes in multiple sclerosis: i. papovaviruses.papovaviruses appear to be neurotropic and one, jc virus, is implicated as the cause of one type of demyelinating disease, progressive multifocal leukoencephalopathy. to investigate whether human papovaviruses play a role in multiple sclerosis, radioactively labeled dna from bk virus, human papilloma virus, and simian virus 40 was used as a probe in order to detect related unlabeled dna sequences in dna isolated from multiple sclerosis brain and/or spinal cord. labeled viral probes were denature ...1977196234
identification and characterization of a papillomavirus from birds (fringillidae).from skin papillomas of the chaffinch (fringilla coelebs), a virus has been purified and studied by physicochemical techniques and electron microscopy. the virions measure 52 nm in diameter and are composed of 72 morphological units arranged in a skew t = 7d surface lattice. a sedimentation coefficient of about 300s and a buoyant density of 1.34 g/ml in cscl were determined for the particle. its protein composition resembles that of human papillomavirus, and the circular double-stranded genome m ...1977197046
inverted repetitive sequences in human papilloma virus 1 (hpv-1) dna. 1977201085
specific immunity in patients suffering from recurring warts before and after repetitive intradermal tests with human papilloma virus.the specific humoral and cellular immunity of 22 patients with multiple or recurring warts was studied. after repeated intradermal tests, using an inactivated, purified viral antigen, the responses obtained could be classed into two groups. the first group (10 patients) was characterized immunologically by the acquisition of a specific cellular immunity and the appearance of circulating igg antibodies, and clinically by a total regression of resolution of the warts two to three weeks after the f ...1977201265
seroepidemiological studies of human papilloma virus (hpv-1) infections.at least two groups of human papilloma viruses can be distinguished serologically: on the one hand hpv 1-3, which are closely related but differ in the restriction enzyme pattern of their dna, and on the other hand hpv-4. the age distributions of patients with warts induced by hpv 1-3 or by hpv-4, respectively, differ markedly. hpv 1-3 predominates between 5 and 15 years of age, whereas hpv-4 could be isolated more often between the ages of 20-25 years. the large number of hpv-1-3-induced warts ...1978203545
characterization of human papilloma virus (h.p.v.) present in genital warts. 1978205177
specific immunity to human papilloma virus (hpv) in patients with genital warts.a study of human papilloma virus (hpv) specific cellular and humoral immunity in 30 patients with genital warts is reported. by in vivo testing with purified, inactivated plantar wart virus, a cell-mediated immunity to hpv was determined in 60% of patients. circulating antibodies, evaluated by immunofluorescence testing, were rare, but these increased after an intradermal test had been carried out, especially in patients with a positive skin test, suggesting a booster effect. no significant diff ...1978207383
so-called multicentric pigmented bowen's disease. report of a case and a possible etiologic role of human papilloma virus.multicentric pigmented bowen's disease (mpbd) is a bowenoid atypia in the genitocrural region with peculiar clinical appearances. a 36-year-old japanese female patient showed a variety of lesions. clinically the lesions on the external genitalia consisted of brown-black papillomatous eruptions, black discrete or confluent papules, and whitish macerated papules. histologically only black papules showed bowenoid atypia, but whitish papules also showed transient bowenoid atypia. electron microscopi ...1978211056
seroepidemiologic studies of bovine papillomavirus infections.bovine and human sera were analyzed for the presence of antibodies against bovine papillomavirus types 1 and 2 (bpv 1 and 2) and human papillomavirus type 1 (hpv 1) in a solid-phase radioimmunoassay. human sera did not react with bpv antigens, and bovine sera showed no evidence of antibodies against hpv 1. in contrast, 19% of all bovine sera tested reacted with bpv 1 and 2 antigens, and 35% of human sera revealed antibodies against hpv 1. no serologic evidence was obtained for heterologous infec ...1979220453
localization of viral dna-replication in sections of human warts by nucleic acid hybridization with complementary rna of human papilloma virus type 1.using complementary rna of human papilloma virus type 1 (hpv1) and in situ hybridization techniques the localization of viral dna replication was studied in sections of 38 human virus acanthomata from 31 different patients. in five cases significant labeling was detected by autoradiography. labeling started always in the first or the second suprabasal cell layer and was strongly limited to the nuclei. a remarkable early beginning of the vacuolated process seemed to be correlated with the visible ...1979220921
connection between capsomeres in human papilloma virus. 1979223316
characterization of human papillomavirus 3 in warts of a renal allograft patient.multiple flat wart-like lesions of a renal allograft recipient were shown to contain hpv 3 or a serologically crossreacting virus by indirect immunofluorescence with monospecific animal antisera against hpv [1--5]. the patient's serum revealed virus specific antibodies (immunofluorescence titer 1/80). papillomaviruses were isolated and after in vitro iodination 3 major proteins (mw 70.000, 56.000 and 43.000) were detected by sds polyacryalmide gel electrophoresis. dna was extracted from the wart ...1979227966
immunology of human warts.rapid advances have occurred in the characterization of human papilloma virus (hpv) types applying the new advanced techniques of restriction endonuclease analysis and molecular hybridization to human wart virus. human papilloma virus can no longer be viewed as a single, homogeneous virus producing all varieties of clinical warts. at least three antigenically heterogeneous hpv types have been associated with common and plantar warts. two additional hpv types have been found in patients with epid ...1979229134
conserved polynucleotide sequences among the genomes of papillomaviruses.the dnas of different members of the papillomavirus genus of papovaviruses were analyzed for nucleotide sequence homology. under standard hybridization conditions (tm - 28 degrees c), no homology was detectable among the genomes of human papillomavirus type 1 (hpv-1), bovine papillomavirus type 2 (bpv-2), or cottontail rabbit (shope) papillomavirus (crpv). however, under less stringent conditions (i.e., tm - 43 degrees c), stable hybrids were formed between radiolabeled dnas of crpv, bpv-1, or b ...1979232171
detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization.human papillomavirus is associated with a variety of anogenital lesions, including genital warts, precancers and cancers. in male patients human papillomavirus has been identified in proliferative lesions ranging from penile and urethral warts to penile and prostatic cancers. we examined the association of human papillomavirus deoxyribonucleic acid (dna) in 84 prostate tissue specimens. specimens were selected from radical prostatectomy, transurethral resection or transrectal biopsy procedures. ...19921279224
prognostic factors in invasive cervical carcinomas associated with human papillomavirus (hpv). quantitative data and cytokeratin expression.as a part of a larger programme to search for the prognostic factors in cervical cancer, quantitative morphometry, demonstration of agnors and expression of different cytokeratin polypeptides (sk2-27, sk1, a 53-b/a2) were used to study a series of 85 cervical squamous cell carcinomas, previously analysed for the presence of human papillomavirus (hpv) dna by in situ hybridization and polymerase chain reaction (pcr). the following nuclear profile parameters were calculated: nuclear area, perimeter ...19921280356
human papillomavirus in oral verrucal-papillary lesions. a comparative histological, clinical and immunohistochemical study.the purpose of this study was to determine the presence of human papillomavirus (hpv) virions in the verrucal-papillary (ovp) lesions by clinical, histological and immunohistochemical [peroxidase-antiperoxidase (pap) features]. thirty seven positive cases were compared with 47 negative cases. considerable overlapping of clinical and histological features can be seen among all these lesions. the study provides evidence that there are no clinical or histologic features with taken alone are strongl ...19921281537
[interferon, interferon inducers, human papillomavirus infection]. 19921285258
replication and persistence of hpv dna in cultured cells derived from laryngeal papillomas.we have investigated the replication and persistence of human papillomavirus (hpv) type 6 and 11 dna in cultured cells derived from laryngeal papillomas, with paradoxical findings. measured by bromodeoxyuridine incorporation into heavy/light dna separated on a cesium chloride gradient, viral dna replicates in both primary and secondary cells. the ratio of the fraction of replicated viral to replicated cellular dna was equal to or greater than 1 in all but one case and was closer to 2 in primary ...19921309272
human papillomavirus type 42: new sequences, conserved genome organization.we report the nucleotide sequence and genome organization of the human papillomavirus type 42. hpv42 dna was isolated from vulvar papillomas. it has been detected in benign forms of proliferative lesions only. the genome of hpv42 is 7917 bp long and shows the open reading frame pattern conserved in all hpvs sequenced so far. hpv42 has no high degree of sequence homology to any of the known hpvs. it shows characteristics previously found either exclusively in hpvs associated with invasive carcino ...19921309278
differences in the integration pattern and episomal forms of human papillomavirus type 16 dna found within an invasive cervical neoplasm and its metastasis.human papillomavirus (hpv) type 16 dna was found in three separate neoplastic lesions within a female patient. the physical state of the viral dna in each lesion was determined by two-dimensional agarose gel electrophoresis. the primary cervical tumor contained large amounts of several distinct episomal forms as well as integrated hpv dna. metastatic tumor tissue found in the vagina had greatly reduced levels of episomal dna and a viral dna integration pattern that was different from that of the ...19921309279
the first 124 nucleotides of the e7 coding sequences of hpv16 can render the hpv11 genome transformation competent.the human papillomavirus (hpv) type 16 is associated with the majority of cervical tumors and is capable of oncogenically transforming cells in culture. hpv type 11 is rarely associated with malignant lesions and does not transform cells in vitro. while the e7 of hpv16 is necessary and sufficient for transformation of rodent cells, the e7 gene of hpv11 is not transforming. in the present report we demonstrate that the hpv11 genome, with the first 124 nucleotides of the hpv16 e7 open reading fram ...19921309281
evaluation and follow-up of abnormal pap smears.an estimated 13,500 cases of invasive cervical cancer and 6,000 related deaths occur each year in the united states. these numbers can be significantly reduced if all women are regularly screened with papanicolaou (pap) smears and undergo colposcopy when abnormalities are detected. screening with pap smears should begin at age 18 or at the age of first sexual intercourse and should be repeated every one to three years, depending on individual risk factors, until age 65. screening may be disconti ...19921309403
clinical effects of photodynamic therapy on recurrent laryngeal papillomas.thirty-three patients with moderate to severe recurrent laryngeal papillomatosis underwent photodynamic therapy at our institution. all received 2.5 mg/kg of dihematoporphyrin ether intravenously either 48 or 72 hours prior to photoactivation with an argon pump dye laser system. photosensitivity was the only side effect seen. statistical analysis showed a significant decrease, by approximately 50%, in the average rate of laryngeal papilloma growth following treatment. the response was especially ...19921309420
the comparative test performance of dot filter hybridization (viratype) and conventional morphologic analysis to detect human papillomavirus.to investigate the test performance of a commercially available detection kit for human papillomavirus (hpv), the relationship between the detection of hpv by dot filter hybridization (dfh) and by standard morphologic methods was studied. four hundred two cervical samples taken from 381 patients referred to a colposcopy clinic were examined. human papillomavirus dna sequences were identified and typed using commercially available anti-sense rna probes. simultaneous cytologic smears were obtained ...19921309483
human papillomavirus types 16 and 18 are not involved in human prostate carcinogenesis: analysis of archival human prostate cancer specimens by differential polymerase chain reaction.human papilloma viruses (hpv) have been implicated in the pathogenesis of a variety of malignancies, especially in carcinomas of the female genital tract. recently, based on observations using the polymerase chain reaction amplification assay, hpv types 16 and 18 specific dna sequences have been detected in prostate cancer specimens obtained by transurethral resection. since hpv types 16 and 18 have been shown to possess oncogenic potential, an association between hpv infection and prostatic car ...19921309581
[inverted papilloma of the nose and paranasal sinuses. apropos of 10 cases].authors report a retrospective study of 10 new cases of inverted papilloma of the nose and paranasal sinuses, observed during a 26 years period, with a 6.1 years average follow up. this group is marked by 2 cases of directly carcinomatous association, 4 cases of recurrence and 2 cases of later malignant transformation. the responsibility of different types of human papillomavirus in the origin of this tumor and its malignant transformation is suspected. preoperative evaluation is based on clinic ...19921298176
histological diagnosis of human papillomavirus (hpv)-related dysplasia: quality control by in situ hybridization (ish) analysis.the authors studied 54 cervical biopsies using in situ hybridization (ish) to identify hpv related lesions. 26 biopsies lacked clear cut koilocytotic atypia on histological examination and 28 had a diagnosis of grades 1 and 2 intraepithelial neoplasia. histological diagnosis of hpv-related dysplasia were studied using ish with biotinylated probes (phatogene dna probe assay enzo diagnostics). probes included hpv types 6/11, 16/18 and 31/35/51. hpv dna were detected in 18 of 28 (64.3%) cervical bi ...19921283127
a t-helper cell epitope overlaps a major b-cell epitope in human papillomavirus type 18 e2 protein.cultivated cd4+ t-helper cells from two patients with cervical adenocarcinoma showed responses to a peptide ektgiltvtyhsetqrtk derived from an e2 protein of human papillomavirus type 18 (hpv 18), but not to a corresponding hpv 16 peptide (hksaivtltydsewqrdq). serum antibodies in the hpv 18 peptide were also demonstrated in these patients. the gilt motif resembles a common pattern present in many t-cell epitopes, and is located at the beginning of an 11-amino acid-long a-helix structure close to ...19921282020
failure to produce warts on human skin grafts on 'nude' mice.split-thickness human skin was exposed to a suspension of human papilloma virus containing 10(11)-10(12) particles/ml, by immersion for varying lengths of time. it was then grafted onto the backs of congenitally athymic 'nude' mice. all grafts were accepted, but no warts developed during an observation period of up to 9 weeks.1976779819
antibody-mediated neutralization of human papillomavirus type 11 (hpv-11) infection in the nude mouse: detection of hpv-11 mrnas.the nu/nu mouse xenograft is the only experimental system permitting the growth of human papillomaviruses (hpv). previous studies demonstrating inhibition of hpv-11 infection by antibodies against hpv-11 virions have used indirect markers of infection, such as graft size and histopathologic features. the presence of hpv-11 mrnas was used as a direct marker of infection: infectious hpv-11 was incubated with rabbit serum raised against purified hpv-11 virions or with the corresponding preimmune se ...19921309849
human papillomavirus type 1 e4 proteins differing by their n-terminal ends have distinct cellular localizations when transiently expressed in vitro.two major human papillomavirus type 1 (hpv-1) e4 proteins are found in large amounts in productively infected differentiating wart cells, a 17-kda protein translated from an e1-e4 transcript and a processed 16-kda protein lacking the e1 amino acids at least. the functions of the e4 proteins are still unknown. we have designed an in vitro system allowing the transient expression of three forms of hpv-1 e4 proteins: the 17-kda e1-e4 protein, an e4 protein without the five e1 amino acids (e4-3200), ...19921309915
a comparison of risk factors in juvenile-onset and adult-onset recurrent respiratory papillomatosis.the clinical triad of a firstborn delivered vaginally to a young (teenage) mother has been previously noted among juvenile onset recurrent respiratory papillomatosis (jo-rrp) patients. this study was based on a questionnaire survey of jo-rrp patients, adult onset recurrent respiratory papillomatosis (ao-rrp) patients, and juvenile and adult controls. the survey results revealed that the complete or partial triad was observed in 72% of jo-rrp patients, 36% of ao-rrp patients, 29% of juvenile cont ...19921309932
characteristics of the lesions and risk of malignant conversion associated with the type of human papillomavirus involved in epidermodysplasia verruciformis. 1979218721
epidermodysplasia verruciformis versus disseminated verrucae planae: is epidermodysplasia verruciformis a generalized infection with wart virus?recently it has been shown that epidermodysplasia verruciformis is induced by human papilloma/virus different from the papilloma/virus of warts, and that 2 distinct viruses-designated hpv-3 and hp-4--are responsible for it. ten cases of epidermodysplasia verruciformis were found to have been caused by hpv-3. clinically and histologically, as well as in the often depressed cell-mediated immunity they closely resembled long-standing verrucae planae, also caused by hpv-3. contrariwise, in epidermod ...1979217936
[malignancies induced by human papilloma virus]. 1978214825
mastomys natalensis papilloma virus (mnpv), the causative agent of epithelial proliferations: characterization of the virus particle.a virus (mnpv) with the structural characteristics of papilloma viruses was isolated from benign and malignant proliferations of adult animals of the inbred line 'gra giessen' of mastomys natalensis. the particles can be banded in cscl gradients at densities of 1.34 g/ml (full particles) and 1.29 g/ml (empty particles). the virus dna has a buoyant density of 1.7104 g/ml and can exist in three different conformations (supercoiled circular, nicked circular and linear), the sedimentation values of ...1978214519
the phylogenetic relationship and complete nucleotide sequence of human papillomavirus type 35.the 7851-bp nucleotide sequence of human papillomavirus (hpv) type 35 was determined. hpv 35 is associated with high-grade cervical intraepithelial neoplasia and invasive carcinomas. from the hpv 35 sequence, open reading frames encoding putative proteins e6, e7, e1, e2, e4, e5, l2, and l1, common to other mucosal hpv types, were identified. structural and control elements present in the long control region (lcr) conserved among other mucosal hpv types were also present in hpv 35. analysis of th ...19921310198
[warts, viruses, cancer (author's transl)].the malignant transformation of cutaneous lesions in epidermodysplasia verruciformis (e.v.), which lesions are caused by a human papilloma virus (hpv), can be compared to that experimentally induced by the polyoma virus. thus, hpv would undergo a lytic, transformant and/or abortive cycle in epidermal cells. in this latter case, it would increase cell dna synthesis and there would be production of cellular antigens coded by the virus. this production of cellular antigens would be the result of th ...1978213005
amplification of human papillomavirus type 16 transforming genes from cervical cancer biopsies and lymph nodes of hungarian patients.we have used a polymerase chain reaction (pcr) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (hpv 16) dna. of the 75 cervical specimens tested, 36 (48%) were positive for hpv 16 in the pcr. a total of 65 pelvic lymph nodes removed during radical surgery on 35 women were also analyzed. lymph nodes originating from 19 patients whose cervical biopsy specimens were negative for hpv 16 seemed to lack hpv 16 sequences. for 16 women ...19921310330
immunological studies in epidermodysplasia verruciformis.immunofluorescence and cell mediated immunity studies have been performed in 14 cases of epidermodysplasia verruciformis (ev), 3 of those abortive or regressing in members of the families of the patients with ev. two different types of human papillomavirus (hpv)--hpv3 and hpv4--have been found in cases of ev. hpv3 was detected also in flat warts without features of ev. there was no cross-reactivity between these two viruses, neither with hpv1 responsible for plantar warts nor with hpv2 inducing ...1978212145
an immunoelectron microscopic localization of wart associated antigens present in human papilloma virus (hpv) infected cells.wart associated antigens were observed by the indirect immunoperoxidase technique in light and electron microscopy. positive reaction products could be found in the nuclei of wart cells, in which virus particles were labeled with a specific immune rabbit serum and with human sera from patients with warts. tissue antigens, differing from the viral labeled inclusions, were detected within the cytoplasm of some infected cells, by means of igm and igg antibodies from patients with warts. in these pa ...1978205617
human papillomavirus--related diseases in the female patient.human papillomavirus (hpv) has been strongly associated with malignancy in the female lower genital tract. because squamous-cell carcinoma of the cervix is preceded by a spectrum of easily detectable and treatable premalignant changes, it is very preventable. the management of the patient with an abnormal papanicolaou smear and the treatment of cervical, vaginal, and vulvar disease are outlined.19921310548
characterization of a new type of human papillomavirus that causes skin warts.a human papillomavirus (hpv) was isolated from the lesions of a patient (ml) bearing numerous hand common warts. this virus was compared with the well-characterized hpv found in typical plantar warts (plantar hpv). ml and plantar hpv dnas have similar molecular weights (5.26 x 10(6) and 5.23 x 10(6), respectively) but were shown to be different by restriction enzyme analysis. when the cleavage products of both dnas by endonuclease ecori, bami, hpai, or hind were analyzed by electron microscopy, ...1977198572
comparative study of delayed hypersensitivity skin reactions and antibodies to human papilloma virus (hpv). 1977198169
koilocytosis in neoplasia of the urinary bladder.koilocytosis is commonly regarded as indicative of human papilloma virus infection in the uterine cervix. in 1987 morphologically similar changes were reported in bladder tumours. this is confirmed in the present study, the incidence here being 65%. in addition the incidence of koilocytic change was shown here to increase from non-infiltrative who grade i to infiltrative who ii lesions, and to be more common in bladder lesions in women with cervical koilocytosis. the latter is in keeping with ou ...19921310631
analysis of the structure of human papilloma virus dna.human papilloma virus dna has been examined for heterogeneity by electron microscopy, reassociation kinetics, and restriction endonuclease digestion. no heterogeneity in contour length was detected. the reassociation rate of papilloma dna isolated from pooled warts was consistent with that expected for a homogeneous, unique dna of molecular weight 5 x 10(6). restriction endonuclease digestions of five papilloma dna preparations isolated from pooled warts yielded predominantly fragments of the ex ...1977196580
evidence for antigenic determinants shared by the structural polypeptides of (shope) rabbit papillomavirus and human papillomavirus type 1. 197884435
an in vivo study of cell-mediated immunity in human warts. preliminary results.purified human papilloma virus (hpv) was used to study cell-mediated immunity (cmi) in patients with warts and in controls by means of the intradermal test (idt). idt was positive in 75% of patients with past history of warts, in 56% of patients presenting with warts, but in only 7% of controls. immunofluorescent antibody levels were initially low and increased after injection of hpv antigen in all groups.197770923
human papillomavirus dna in women without and with cytological abnormalities: results of a 5-year follow-up study.to determine the prevalence of hpv 6, 11, 16, and 18 in a population without cytological or histological abnormalities, the cervical smears of women attending three clinics in germany were screened over the past 5 years. the filter in situ hybridization method was used throughout. a total of 20,161 smears, taken from 11,667 women, were tested. when the results of only the first examination are considered, 8.8% (950/10,778) of women with normal cytology were positive for hpv dna. if we divide the ...19921309717
occurrence of human papillomavirus dna in primary lung neoplasms.the occurrence of human papillomavirus (hpv) dna in primary lung carcinomas and in squamous metaplasia of the bronchus was studied using in situ hybridization techniques and commercially available biotinylated dna probes to hpv subtypes 6/11, 16/18, and 31/33/35. the authors found hpv dna in six of 20 cases of squamous cell carcinoma and one of six cases of large cell undifferentiated carcinoma. there were two cases each of the 6/11 serotypes and the 16/18 serotypes and three cases of the 31/33/ ...19921309679
chromosome alterations contribute to neoplastic progression of transformed rat embryonal fibroblasts.three types of transformants derived from rat embryonal fibroblasts (refs) corresponding to the different progressional stages were obtained: tf1 (human papillomavirus type 16 e7 (hpv16 e7) transfection alone) and tf2 (e7 plus adenovirus type 12(ad12) e1b were immortalized, tf3 (e7 plus adenovirus type 5 (ad5) e1b) was anchorage-independent but not tumorigenic, and tf4 (e7 plus ej-ras) was tumorigenic. cytogenetic investigations revealed that the cells carrying specific chromosomal abnormalities ...19921309485
detection of human papillomavirus dna in carcinomas of the nasal cavities and paranasal sinuses by polymerase chain reaction.the authors retrospectively searched for human papillomavirus (hpv) types 16 and 18 in 60 cases of carcinoma arising from the nasal cavities (nc) and paranasal sinuses (ps) by using the polymerase chain reaction (pcr) on dna extracted from formalin-fixed, paraffin-embedded tissues. in cases of scc (n = 49), the authors also compared the clinical features of patients with hpv-positive and hpv-negative results to determine the clinical significance of hpv. hpv 16 and 18 were detected in 7 of the 4 ...19921309430
failure to detect human papillomavirus dna in extramammary paget's disease.ten genital skin specimens, biopsy proven to be paget's disease, were examined by human papillomavirus (hpv) in situ hydridization in an effort to detect dna of hpv types 6, 11, 16, 18, 31, 33, and 35. all ten specimens showed no evidence of dna of these hpv types. extra-mammary paget's disease is probably not a result of infection with hpv types 6, 11, 16, 18, 31, 33, or 35.19921309305
transcriptional activation of several heterologous promoters by the e6 protein of human papillomavirus type 16.the e6 protein of human papillomavirus type 16 (hpv-16), along with e7, is responsible for the hpv-induced malignant transformation of the cervix. however, the mechanism of this transformation activity is not well understood. we investigated whether the entire e6 protein of hpv-16 could act as an activator of transcription. experiments in which nih 3t3 cells were cotransfected with an e6 expression vector together with the reporter chloramphenicol acetyltransferase (cat) gene linked to various m ...19921309249
detection of human papillomavirus dna sequences in tongue squamous-cell carcinoma utilizing the polymerase chain reaction method.twenty-four cases of tongue squamous-cell carcinoma (scc) were analyzed for human papillomavirus (hpv) dnas by the polymerase chain reaction (pcr) method and the dot-blot hybridization technique. hpv dnas were detected in 8 cases. one specimen histopathologically diagnosed as poorly differentiated grade-iii scc contained both hpv-16 and hpv-18 dna, and 7 other cases contained hpv-16 dna.19921309724
molecular analysis of different allelic variants of wild-type human p53.the p53 tumour suppressor gene is intensively studied because mutations in this gene are the most common genetic alteration so far identified in human cancer. considerable emphasis has thus been placed on characterizing the biological differences between mutant and wild-type p53 protein. this has led to the realization that in cultured cells, mutant p53 behaves like an oncogene, whereas wild-type p53 is a tumor suppressor gene. the p53 protein is also a target for the tumour virus oncogene produ ...19921297328
human papillomavirus dna in tissues and ocular surface swabs of patients with conjunctival epithelial neoplasia.dna from human papillomavirus (hpv) type 16 has been recently identified in conjunctival epithelial dysplasia and carcinoma. in other body sites, hpv 16 is thought to play a role in the development of dysplastic lesions. to further explore the relationship between hpv and conjunctival neoplasia, we examined paraffin-embedded tissue samples from 42 biopsies or excisions from 38 patients whose lesions ranged from mild dysplasia to infiltrating squamous carcinoma of the conjunctiva. we also examine ...19921309728
human papillomavirus type 16 e5 gene stimulates the transforming activity of the epidermal growth factor receptor.we have until recently made several unsuccessful attempts to assign any activity to the human papillomavirus type 16 (hpv-16) e5 gene product. however, studies with the bovine papilloma virus 1 (bpv-1) e5 protein indicated an interaction with the epidermal growth factor receptor (egfr). in light of the overall similarity between the hpv and bpv e5 proteins we attempted to determine whether the hpv-16 e5 gene had any common activity. in cells expressing high levels of egfr plus hpv-16 e5 we found ...19921311063
during negative regulation of the human papillomavirus-16 e6 promoter, the viral e2 protein can displace sp1 from a proximal promoter element.the principal early promoter of human papillomaviruses (hpvs), designated p97 in the case of hpv-16, contains four characteristically aligned cis-responsive elements, namely one binding site for sp1, two for the viral e2 proteins, and the tata box. the sp1 binding site is needed to mediate activation of p97 by the remote epithelial-specific enhancer, and the two e2 binding sites contribute to a negative feedback-loop of viral gene expression. the sp1 consensus motif and the tata-box distal e2 bi ...19921311070
specific n-methylations of hpv-16 e7 peptides alter binding to the retinoblastoma suppressor protein.complex formation between the human papilloma virus type 16 e7 protein (hpv-16 e7) and the retinoblastoma growth suppressor protein (rb) is believed to contribute to the process of cellular transformation that leads to cervical carcinoma. genetic analysis of the hpv-16 e7 protein has shown that the segment of e7 homologous to the conserved region 2 of adenovirus 5 e1a protein is involved in both rb binding and e7-mediated cell transformation. we have previously shown that a peptide colinear with ...19921309779
comparative study of in situ hybridization and immunohistochemical techniques for the detection of human papillomavirus in lesions of the uterine cervix.among the techniques currently used for the detection of human papillomavirus (hpv) in genital lesions, only two correlate hpv with the histopathological findings of the lesion: immunohistochemistry and in situ hybridization. consequently, we were prompted to carry out a comparative study on both techniques to check their utility and efficacy as routine diagnostic methods. 52 biopsy specimens of uterine cervix diagnosed histopathologically as condylomas and cervical intraepithelial neoplasia+koi ...19921281009
human papillomavirus infection in esophageal squamous-cell carcinoma in western countries.recent studies have suggested that esophageal hpv infection could be a risk factor for esophageal squamous-cell carcinoma. the aims of our study were to assess the presence of hpv esophageal infection among french patients with esophageal squamous-cell carcinoma and to compare the prevalence of this infection among control patients exposed to similar known risk factors (alcohol and tobacco) and among non-exposed control patients. all patients had the following investigations: serum immunoglobuli ...19921311284
human papillomavirus type 6a dna in the lung carcinoma of a patient with recurrent laryngeal papillomatosis is characterized by a partial duplication.transcriptionally active human papillomavirus type 6a (hpv-6a) dna was detected in a lung carcinoma of a patient with recurrent laryngeal papillomatosis. the carcinoma contained episomal hpv-6a genomes that had a duplication of the upstream regulatory region, the late region and a portion of the early region. hpv-6a genomes found in benign laryngeal papillomas from the same patient did not contain this duplication. a role for the mutant molecules in the pathogenesis of the malignancy is suggeste ...19921311363
squamous papilloma of the esophagus associated with the human papillomavirus.squamous cell papilloma of the esophagus is a rare lesion involving less than 60 case reports worldwide. these lesions are generally asymptomatic but may at times grow and spread rapidly. one fatality, a result of massive dissemination, has been reported. until recently, human papillomavirus had not been identified in association with esophageal papillomas. a second case, to the authors' knowledge, of esophageal papillomas associated with human papillomavirus is reported. the virus has been prev ...19921310082
detection of human papillomavirus in squamous neoplasm of the penis.infection of the external human urogenital system with human papillomavirus has been implicated with the development of genital cancer. a modified polymerase chain reaction technique has been used to evaluate type specific deoxyribonucleic acid (dna) sequences of unique e6 to e7 transforming regions of human papillomavirus genomes (types 6b/11, 16 and 18) in a morphological spectrum of in situ (carcinoma in situ) and invasive neoplasm of the penis. we studied 15 examples of carcinoma in situ [7 ...19921310122
recent progress in defining the epidemiology of human papillomavirus infection and cervical neoplasia. 19921311392
determination of the functional difference between human papillomavirus type 6 and 16 e7 proteins by their 30 n-terminal amino acid residues.human papillomavirus type 16 (hpv 16) is often found in cervical carcinomas, while hpv 6 is frequently associated with benign genital lesions. we have compared the abilities of the e7 transforming proteins of hpv 6 and 16 to transform various established and primary rodent cells by using the same heterologous promoter system. hpv 16 e7 efficiently induced anchorage-independent growth of all the rodent cell lines tested and immortalized or cooperated with ras in transforming primary rat cells. on ...19921310180
differences in transforming activity and coded amino acid sequence among e6 genes of several papillomaviruses associated with epidermodysplasia verruciformis.using the polymerase chain reaction technique, we cloned and sequenced dna fragments containing the e6 genes of the epidermodysplasia verruciformis (ev)-associated hpvs 5, 8, 14, 20, 21, 25, and 47, of which only the sequences of hpvs 5, 8, and 47 have previously been reported. based on the deduced amino acid sequence homology (57.3 to 83.0%), these hpvs could be divided into two clusters: hpvs 5, 8, and 47, and hpvs 14, 20, 21, and 25. the e6 genes of three hpvs from each cluster were examined ...19921310189
possible transplacental transmission of human papillomaviruses.the objective of this study was to examine the possibility of intrauterine human papillomavirus infection of fetuses by transplacental transmission of human papillomavirus before delivery.19921310201
human papillomavirus dna detection in papanicolaou-stained cervical smears with a nonradioactive, in situ hybridization assay.archived papanicolaou-stained cervical smears from women with different cervical pathologies were processed for human papillomavirus (hpv) dna detection and typing with an in situ hybridization (ish) assay that employed commercial biotinylated hpv dna probes. two hpv dna probes were utilized: one included hpv genotypes 6/11 and the other, 16/18. the method yielded positive results for hpv dna 6/11 in 5 cases with condylomata acuminata (100%) and in 2 of 47 with flat warty lesions (4.2%), whereas ...19921311885
detection of antibodies to a linear epitope on the major coat protein (l1) of human papillomavirus type-16 (hpv-16) in sera from patients with cervical intraepithelial neoplasia and children.antibodies to the major (l1) coat protein of human papillomavirus type 16 (hpv-16) in sera from patients with cervical intra-epithelial neoplasia (cin) have been investigated by means of recombinant proteins and synthetic peptides. when l1-hpv-16 fusion proteins were used in immunoblot assays, no antibody reactivity was found in sera from 52 patients with cin or from 21 unrelated children. amino-acid sequence analyses indicated that l1-hpv-16 amino acids 473 to 492 may contain an hpv-16 type-res ...19921310487
clinical applications of molecular biologic screening for human papillomavirus: diagnostic techniques. 19921311996
prevalence of human papillomavirus types 16 and 18 in squamous-cell carcinoma of the penis: a retrospective analysis of primary and metastatic lesions by differential polymerase chain reaction.human papillomaviruses (hpv), particularly types 16 and 18, may be carcinogenic effectors in a variety of human lower-genital-tract malignancies. using the highly sensitive technique of differential polymerase chain reaction (d-pcr) with amplimers from the e6 open reading frames of hpv types 16 and 18, a retrospective analysis of a 20-year institutional experience with squamous-cell carcinoma of the penis (sccp) was performed to determine the prevalence of these hpv types in this malignancy. par ...19921312062
expression and splicing patterns of human papillomavirus type-16 mrnas in pre-cancerous lesions and carcinomas of the cervix, in human keratinocytes immortalized by hpv 16, and in cell lines established from cervical cancers.we have analysed the splicing patterns of human papillomavirus (hpv) type-16 mrnas in a human epithelial cell line immortalized by hpv 16 (hpkii), in cell lines established from cervical carcinomas (siha and caski) and in pre-invasive and invasive carcinomas of the cervix. the presence of mrna species previously described, which could encode the e6, e6i, e6ii, e6iii, e7, e2, e2c, e4, e5 and l1 proteins, was determined, using the rna polymerase chain reaction (pcr) technique with primers that fla ...19921310488
seroreactivity against hpv 16 e4 and e7 proteins in renal transplant recipients and pregnant women. 19921312108
human papillomavirus in the male patient.human papillomavirus is a double-stranded dna virus associated with a broad spectrum of clinical states including condylomata acuminata, latent and subclinical infection (acetowhitening), bowen's disease, and carcinoma of the penis and anus. the virus is transmitted by direct contact with site-subtype specificity; additional studies are needed to elucidate the exact transmissibility and disease course of hpv infection. the association of hpv-16 and hpv-18 with anogenital malignancy increases the ...19921310549
the e7 functions of human papillomaviruses in rat 3y1 cells.among more than 60 human papillomavirus (hpv) genotypes, several hpvs are believed to be high risk because they are found in close association with cervical carcinoma. we compared the e7 genes from hpvs 1, 6b, 16, 18, and 33 for their transactivating, transforming, and mitogenic functions in a single cell line rat 3y1. whereas both the low-risk (1 and 6b) and the high-risk (16, 18, and 33) hpvs were transactivating for the adenovirus e2 promoter, only the high-risk hpvs were capable of focal tra ...19921310552
antibodies to hpv-16 e6 and e7 proteins as markers for hpv-16-associated invasive cervical cancer.transforming proteins e6 and e7 of human papillomaviruses (hpvs) are consistently expressed in hpv-associated cervical cancers. in elisa with four hpv-16 e6-e7 peptides, patients with hpv-16-associated invasive cervical cancer (group 1) had a greater seroreactivity than all other groups, which included patients with hpv-16-associated cervical intraepithelial neoplasia, invasive cervical cancer patients without hpvs, and unaffected controls. a larger proportion of group 1 sera, as compared to ser ...19921312268
human papillomavirus and the three group metaphase figure as markers of an increased risk for the development of cervical carcinoma.in this study, the presence of atypical mitotic figures and human papilloma virus (hpv) genomes was related to the degree of cervical intraepithelial neoplasia (cin) or microinvasive carcinoma (mic) as found in 94 paraffin-embedded biopsies from cervical lesions. the results showed that the frequency of three group metaphase (tgm) figures, a special kind of atypical mitotic figure, as well as the presence of hpv 16 and 18 genomes increased with the degree of cervical intraepithelial neoplasia. t ...19921310833
laboratory techniques in the investigation of human papillomavirus infection. 19921312507
in situ hybridization and laryngeal papillomas.the technique of in situ hybridization with biotin-labeled probes was applied to 20 patients with laryngeal papillomatosis (9 with adult-onset disease and 11 with juvenile-onset). ten patients were noted for epithelial transformation of their laryngeal disease. all patients, regardless of their clinical outcome, tested positive for human papillomavirus group 6/11. the biotin method of in situ hybridization proved to be a sensitive method in this study, identifying the infecting viral group in 23 ...19921310841
molecular variants of human papillomavirus type 16 from four continents suggest ancient pandemic spread of the virus and its coevolution with humankind.we have amplified by the polymerase chain reaction, cloned, and sequenced genomic segments of 118 human papillomavirus type 16 (hpv-16) isolates from 76 cervical biopsy, 14 cervical smear, 3 vulval biopsy, 2 penile biopsy, 2 anal biopsy, and 1 vaginal biopsy sample and two cell lines. the specimens were taken from patients in four countries--singapore, brazil, tanzania, and germany. the sequence of a 364-bp fragment of the long control region of the virus revealed 38 variants, most of which diff ...19921312620
the e6 and e7 genes of human papillomavirus type 6 have weak immortalizing activity in human epithelial cells.previous studies have shown that the e7 gene of human papillomavirus (hpv) type 16 or 18 alone was sufficient for immortalization of human foreskin epithelial cells (hfe) and that the efficiency was increased in cooperation with the respective e6 gene, whereas the hpv6 e6 or e7 gene was not active in hfe. to detect weak immortalizing activities of the hpv6 genes, cells were infected with recombinant retroviruses containing hpv genes, alone and in homologous and heterologous combinations. the hpv ...19921312623
the role of human papillomavirus in cutaneous neoplasia. 19921310867
low incidence of human papillomavirus type 16 dna in bladder tumor detected by the polymerase chain reaction.human papillomavirus (hpv) detection was done using the polymerase chain reaction technique on tumor tissue from 44 patients with transitional cell carcinoma of the urinary bladder. only one of the 44 was associated with hpv infection. the hpv-positive patient was not known to have immunodeficiency or genital warts, and the tumor was not morphologically different from the other tumors. control experiments excluded the possibility that this finding was caused by contamination of the sample. this ...19921310888
lack of immortalizing activity of a human papillomavirus type 16 variant dna with a mutation in the e2 gene isolated from normal human cervical keratinocytes.the oncogenic potential of a human papillomavirus type 16 (hpv16) variant cloned from normal human cervical keratinocytes has been tested in vitro using primary rodent epithelial cells and human cervical keratinocytes. the hpv16 variant was able to extend the lifespan of, but failed to immortalize, human keratinocytes. it could however cooperate with an activated ras oncogene to transform primary rodent cells. radioimmunoprecipitation assays of the rodent cells showed that they expressed the e7 ...19921312701
human papillomavirus type 16 and 18 gene expression in cervical neoplasias.human papillomavirus (hpv) types 16 and 18 are strongly implicated in the generation of progressive cervical neoplasms. the viruses produce complex families of overlapping messenger rnas that are linked to differentiation, making it necessary to analyze gene expression in the context of morphology. we have developed hpv type 16 and type 18 subgenomic clones from which 3h-labeled riboprobes specific to individual mrna families can be generated in vitro. using these probes for in situ hybridizatio ...19921310950
demonstration of human papillomavirus dna in a peripheral ameloblastoma by in situ hybridization.the peripheral ameloblastoma (pa) is a rare, extraosseous, odontogenic lesion usually located on the mandibular or maxillary gingiva. it often appears as an asymptomatic, firm, pink-red nodule with occasional secondary involvement of the underlying bone. although histologically identical to primary intraosseous ameloblastoma, the pa is notably less aggressive in biologic behavior and rarely recurs following conservative treatment. it has a proposed dual histogenesis, while its etiology remains o ...19921310952
oral folic acid supplementation for cervical dysplasia: a clinical intervention trial.we attempted to evaluate the effect of oral folic acid supplementation on the course of cervical dysplasia.19921312773
strategies of chemoprevention based on antigenic and molecular markers of early and premalignant lesions of the bladder.using monoclonal antibodies, we have identified a series of tumor-associated antigens selectively expressed on tumor subtypes with distinct clinical behaviours. the mucinous antigen m344 and the gp200 surface antigen 19a211 are preferentially expressed on papillary superficial tumors and carcinoma in situ lesions of the bladder. the combination of these two antigenic markers in immunocytology and flow cytometry studies of exfoliated cells has improved the sensitivity of detection for bladder tum ...19921305695
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