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glomerular deposition of immune complexes in dogs following natural infection with canine adenovirus.the renal lesions were studied in eight dogs which had either died as a result of acute canine adenovirus infection (rubarth's disease) or were in various stages of recovery from the clinical disease. using immunofluorescence techniques granular deposits of igg were detected in the glomeruli of six dogs; four of these animals had similar glomerular deposits of canine adenovirus antigen. eluates obtained from kidney tissue of four dogs were found to contain antiviral antibody. histologically thos ...1975166480
cross-protective immunity to canine adenovirus type 2 by canine adenovirus type 1 vaccination. 1978216291
viral-antibody complexes in canine adenovirus type 1 (cav-1)ocular lesions: leukocyte chemotaxis and enzyme release.canine adenovirus-type 1 (cav-1)-antibody complexes caused severe anterior uveitis with corneal edema ("blue eye") when injected into the anterior chamber of normal dogs. the response of the anterior uvea to such immune complexes (ic) was similar to the spontaneously occurring disease. in the presence of complement (c'), ic caused release of neutrophile chemotactic factors. following phagocytosis of ic-c' leukocytes released lysosomal enzymes, as indicated by the presence of acid phosphatase in ...19751095299
diseases and parasites of red foxes, gray foxes, and coyotes from commercial sources selling to fox-chasing enclosures.fifty-six red foxes (vulpes vulpes), 18 gray foxes (urocyon cinereoargenteus), and 13 coyotes (canis latrans) obtained by the south carolina wildlife and marine resources department during an investigation of suspected illegal wildlife translocation were examined for diseases and parasites. red foxes and coyotes were confiscated from an animal dealer based in ohio (usa), and gray foxes were purchased from an animal dealer in indiana (usa). emphasis was placed on detection of pathogens representi ...19921474656
acute canine adenovirus 2 infection increases histamine airway reactivity in beagle puppies.acute infection with canine adenovirus was studied in 23 specific pathogen-free outbred beagle puppies (median age = 78 days, range = 67 to 86 days) to determine its effects on pulmonary function and airway responsiveness to aerosolized histamine. the following groups were studied: uninoculated (n = 6, control); inoculated with canine adenovirus type 2 (cav2) (n = 11, infected); and subclinical spontaneous infection with cav2 (n = 6, subclinical). while anesthetized with chloralose and mechanica ...19902154153
[purification and properties of the hexon antigen of canine adenovirus serotype cav-1].the main antigen and immunogen of canine adenovirus type 1 (cav-1) has been purified to near homogeneity from cultural fluid of a cav-1-infected primary cell culture by hydrophobic and anion-exchange chromatography. the hexon native form (trimer) was shown to be resistant against denaturation by sds under conditions of sds-page performed without heating the samples. the monomer chain of the cav-1 hexon was apparently identical in terms of electrophoretic mobility with that of the previously sequ ...19902085617
changes in lung mechanics and reactivity with age after viral bronchiolitis in beagle puppies.we measured changes with growth in lung function and airway reactivity after acute canine parainfluenza virus type 2 (cpi2, n = 5), canine adenovirus type 2 (cav2, n = 7), and sequential cav2-cpi2 (n = 6) infections or no infection (controls, n = 6) in beagle puppies (age approximately 79 days). in the cpi2 and cav2 groups, a lower respiratory illness developed by day 3 postinfection with clinical recovery by day 14. in the cav2-cpi2 group, puppies were inoculated initially with cav2 and 12 days ...19901963888
a mutant of canine adenovirus type 2 with a duplication of the e1a region exhibits altered expression of early region 4.the genomic dna of a vaccine strain of canine adenovirus type 2 (vaxitas; ici tasman) has been shown to contain two copies of the e1a region, the second being at the far right end of the genome. dna sequence analysis of the right terminal 2.8 kbp of this vaccine strain showed that numerous point mutations have occurred in the second copy, which would preclude the synthesis of any functional products. however, expression vectors in which the e1a promoter from the right terminus were linked to the ...19911832708
sequence analysis of putative e3 and fiber genomic regions of two strains of canine adenovirus type 1.the nucleotide sequences of the genomes of two strains of canine adenovirus, type 1 (glaxo and cll) have been determined within the region spanning approximately 0.80-0.91 map unit. homology comparisons with other adenoviral dnas indicate that this region contains part of the hexon-associated protein (pviii) precursor gene on the left, and a 57-kda fiber-encoding gene on the right. in both viral strains, the sequences of these genes are identical. accepting the generally highly conserved nature ...19911828920
changes in lung mechanics and histamine responsiveness after sequential canine adenovirus 2 and canine parainfluenza 2 virus infection in beagle puppies.we determined the effects of an immediately antecedent viral lower respiratory tract infection (lri) on the severity of clinical illness, changes in lung function and airway histamine responsiveness produced by a subsequent lri in 9-12 week old beagle puppies inoculated with canine adenovirus 2, followed in 2 weeks by inoculation with canine parainfluenza 2 virus (cav2-cp12, n = 7). we compared their acute responses to puppies infected with cp12 alone (n = 5), cav2 alone (n = 7), and no infectio ...19911654540
prerequisites for oral immunization of free-ranging raccoons (procyon lotor) with a recombinant rabies virus vaccine: study site ecology and bait system development.a model baiting system suitable for the delivery of an oral rabies vaccine to free-ranging raccoons (procyon lotor) was developed and tested on barrier islands in south carolina (usa). features of barrier island physiography and ecology were studied relative to selective bait deployment and site biosecurity. capture-mark-recapture data were obtained from 228 raccoons. raccoon density estimates, using a modified census assessment technique, were one raccoon per 1.8 to 2.7 ha. mean (+/- se) and ra ...19921548804
differences in the e3 regions of the canine adenovirus type 1 and type 2.sequence analysis of a region extending between the pviii and the fiber gene of canine adenovirus type 1 (cav-1, utrecht) and type 2 (cav-2, manhattan) was performed. the results revealed a high level of identity between the two viruses when the pviii gene and the n-terminal part of the fiber gene were compared. the open reading frames of region e3 in of cav-1 and cav-2 encoded a 22 kda and 40.7 kda polypeptide, respectively. the cav-1 and cav-2 e3 region polypeptides shared conserved amino and ...19921534956
experimental oral administration of canine adenovirus (type 2) to raccoons (procyon lotor).canine adenovirus type 2 (cav2) has been proposed for recombinant vaccines to control rabies in wild animals. to evaluate the suitability of cav2 as a safe vector for the genetically engineered vaccines, seven wild-caught raccoons (three males and four females) were administered cav2 per os. two of the animals were euthanatized on each of post-infection days 3, 6, and 14, and one was euthanatized on day 21. two other control raccoons (a male and a female) were also euthanatized on day 21. micros ...19921448897
nucleotide and deduced amino acid sequence of the canine adenovirus type 1 proteinase.the dna sequence of an open reading frame (orf) corresponding to the canine adenovirus type 1 (can1) proteinase gene was determined. a total of 1171 base pairs were sequenced from the downstream end of the hindiii-a can1 genomic fragment, including adjacent regions corresponding to the carboxy-terminal portions of the hexon and the dna-binding proteins. the predicted can1 proteinase consists of 206 residues (23,325 d) of which 68% are identical and 83% are similar to the sequence of the human ad ...19921413543
a serologic survey of the island fox (urocyon littoralis) on the channel islands, california.the island fox is listed as a threatened species in california. a serologic survey of 194 island foxes (urocyon littoralis) was conducted over the entire range of the species on the channel islands (california, usa). antibody prevalence against canine adenovirus and canine parvovirus reached 97% and 59%, respectively, in some populations sampled. antibody prevalence of canine herpesvirus, canine coronavirus, leptospirosis and toxoplasmosis were low. antibodies against canine distemper virus were ...19921318424
multiple enlargements in the right inverted terminal repeat of the dna of canine adenovirus type 2.the manhattan strain of canine adenovirus type 2 (cav 2) was examined. restriction endonuclease analysis and blot hybridization experiments revealed the heterogeneity of the viral dna. at least 9 unequally expanded species of the viral genome have been recognized. this diversity is caused by different enlargements in the right inverted terminal repeat (itr) of the virus. the differences between the individual enlargements were shown to be the different multiples of 150 base pairs. relatedness of ...19921307439
characterization of canine adenovirus type 1 isolated from american black bears.viruses recovered from tissues taken at necropsy from american black bears were examined by use of immunofluorescence with polyclonal and monoclonal antibodies, virus neutralization with monoclonal antibodies, and restriction endonuclease analyses of the viral genomes. with these techniques, viruses were determined to be canine adenovirus type 1. seronegative dogs that were inoculated with the virus had clinical signs typical of infectious canine hepatitis, suggesting that the virus, which was v ...19882840839
corneal endothelium in viral induced anterior uveitis. ultrastructural changes following canine adenovirus type 1 infection.dogs inoculated intravenously with attenuated canine adenovirus type 1 developed anterior segment inflammation and corneal edema. during the stage of mild anterior uveitis, virus was isolated from the aquious fluid, and by electron microscopy, viral replication was found to occur in corneal endothelial cells. later, at the stage of severe anterior uveitis with corneal edema, virus was not isolated from the aqueous fluid and cells containing intranuclear (replicating) virus were not found. at thi ...19751138688
differences in cytopathology between canine adenovirus serotypes. 1979534926
use of canine adenovirus type 2 vaccine to control kennel cough syndrome. 1979223268
studies of respiratory disease in random-source laboratory dogs: viral infections in unconditioned dogs.a prospective study was conducted to identify the viruses causing respiratory diseases in unconditioned, random-source dogs. during the quarantine period, respiratory disease occurred in 86 of 167 (52%) dogs, and 34 (21%) died. most affected dogs had a distemper-like illness which required extensive and prolonged care. histopathologic studies confirmed the diagnosis of canine distemper in 10 of 12 (83%) fatal infections examined. sixty-seven of 91 (74%) dogs which arrived without canine distempe ...1979220463
the pathogenicity of an attenuated strain of canine adenovirus type 2 (cav-2). 1978217139
the canine contagious respiratory disease complex (kennel cough).several infectious agents are involved in the kennel cough complex in dogs. they include canine parainfluenza virus (sv5), canine adenovirus 2, bordetella bronchiseptica, and possibly several mycoplasma species. the importance of each of these agents in the disease syndrome is discussed as well as possible prevention or treatment.1978204455
effect of canine adenovirus on jinet and vero cells. 1977199776
an immunopathologic study of interstitial nephritis associated with experimental canine adenovirus infection.the localisation of canine adenovirus in the renal tubules induces a marked cellular response in the interstitium. a number of mechanisms appear to be involved in the genesis of the lesions. direct lytic damage to tubular epithelial cells by the virus is probably augmented by immunological factors. the production of local anti-viral antibody by plasma cells in the vicinity of virus-containing cells may result in the formation of toxic antigen-antibody complexes while the accumulation of large po ...1976189005
detection of immune complexes in the serum of dogs infected with canine adenovirus.evidence for the presence of circulating virus-immune complexes in dogs experimentally infected with canine adenovirus was found when deposits of canine igg were present in the glomeruli of recipient mice. this passive transfer of complexes occurred only with serum taken at the height of the disease when antibody titres were low and when deposition of complexes in the donor dogs was maximal. transfer of complexes was not obtained early in the disease (no circulating antibody and minimal depositi ...1976181806
canine adenovirus: its role in renal and ocular disease: a review. 1976175219
canine adenovirus type 2-induced immunity to two canine adenoviruses in pups with maternal antibody.twenty-four beagle pups with high levels of maternal antibody to canine adenovirus type 1 (cav-1) and canine adenovirus type 2 (cav-2) were oronasally inoculated with cav-2 at 4 weeks of age. the cav-2 was isolated from pharyngeal swabs on postinoculation days 2 through 6. in spite of the infection, maternal antibody continued to decrease for 4 to 8 postinoculation weeks, and then homologous cav-2 neutralizing antibody and, to a lesser extent, cav-1 neutralizing antibody began to increase. when ...1975168797
antibody response to modified-live canine adenovirus vaccine in african hunting dogs (lycaon pictus).nine hunting dogs were bled pre and post vaccination with modified-live virus vaccine. their antibody responses to canine adenovirus type 1 was measured by an indirect immunofluorescent antibody test. the vaccine proved to be both effective and safe.19911659069
nucleotide sequence of e1 region of canine adenovirus type 2.the nucleotide sequence of the leftmost ecori-c fragment (0 to 11.3%) of canine adenovirus type 2 (cad2) which could transform rodent cells morphologically but required additional sequences from 10 to 32 map units (m.u.) for full expression of its oncogenic potential was determined. the ecori-c fragment contains 3609 nucleotide base pairs (bp) encoding e1a, e1b, and pix genes. although the nucleotide sequence of cad2 e1 shows little homology to those of human ads, the amino acid sequences of the ...19892800332
an enzyme-linked immunosorbent assay for the detection of canine antibodies to canine adenoviruses.an enzyme-linked immunosorbent assay was used to detect canine immunoglobulin g antibodies specific for infectious canine hepatitis virus and the serologically related canine adenovirus type 2. the sequential development of homologous and heterologous antibodies was measured by the enzyme-linked immunosorbent assay and serum neutralization tests in two groups of dogs which were experimentally infected with either infectious canine hepatitis virus or canine adenovirus type 2. both tests were comp ...1979229339
mdbk nuclear factor-binding site of various serotypes of adenovirus dna.a fraction with the ability to bind the terminal fragment of equine adenovirus (ead) dna was prepared from mdbk cell nuclei. the fraction (mdbk nuclear factor) bound to the terminal fragment of all human and animal adenovirus dnas examined except avian adenovirus eds-76. however, the terminal fragments of two animal adenoviruses, ead and bovine adenovirus type 3 (bad3), showed higher affinity for the nuclear factor than the others. the mdbk nuclear factor-binding site determined by footprinting ...19863796315
large and small plaque variants of canine adenovirus 2 isolate. 19892557478
localization of inflammation and virions in canine adenovirus type 2 bronchiolitis.beagle puppies develop bronchiolar inflammation and histamine hyperresponsiveness with canine adenovirus type 2 (cav2) infections. we determined the distribution of bronchiolar lesions and correlated inflammation with virions and bronchoalveolar lavage fluid (balf) cytology. nineteen beagle puppies were inoculated with tissue culture fluid (control puppies, n = 8), or cav2 (cav2, n = 11). the puppies had clinical assessments and measurements of lung resistance (rl), and dynamic compliance (cdyn) ...19902167625
localization of canine adenovirus capsid antigens in a mdck cell line by immunoferritin and immunofluorescent techniques. 19724115332
characterization of the dna of canine adenovirus by restriction enzyme analysis.the dna of canine adenovirus (canav 1, strain behring h.c.c. 269) was characterized in detail. the molecular weight of canav dna was (20.1 +/- 0.1) x 10(6) daltons as determined by contour length measurements, and 19.9 x 10(6) daltons as determined by restriction enzyme analysis. a terminal protein was found to be covalently linked to the 5' ends of canav dna. physical maps of viral dna were constructed for bamhi, clai, saci, sali, and smai enzymes. three international strains of canav [american ...19852982757
identification and nucleotide sequence of the early region 1 from canine adenovirus types 1 and 2.the genome of canine adenovirus type 1 (cav-1) has been cloned and restriction maps compiled. these maps are compared with those of canine adenovirus type 2 (cav-2). the left ends of both genomes were further characterised by dna sequence analysis. several features of the dna sequence and predicted polypeptide sequence are similar to those of the human adenoviruses. the level of homology observed across the e1 regions appears to be of the same order as the overall dna similarity between cav-1 an ...19892623943
t antigens of bovine and canine adenoviruses demonstrated by immunofluorescence.the intracellular development in acutely infected cells of bovine and canine adenovirus t antigens was followed by immunofluorescent staining. with both species of adenovirus, antigen was first detected as intranuclear pin-points at 18 hr postinfection and coalescence into large lobular masses was noticed by 24 hr. cross-reactions between bovine 1 (nononcogenic) and bovine 3 (oncogenic) t antigens were not observed by the direct technique although the more sensitive indirect procedure did reveal ...19694240433
serologic comparisons of infectious canine hepatitis and toronto a26-61 canine adenoviruses. 19694307984
vaccine-associated canine distemper infection in a litter of african hunting dogs (lycaon pictus).four, 57 days old, african hunting dog puppies (lycaon pictus) from one litter died within three weeks following vaccination with modified-live canine distemper virus (cdv) and killed canine adenovirus type 1, canine parvovirus and leptospira icterohemorrhagiae and canicola. 18 days post vaccination, the animals developed neurologic disease characterized by episodes of grand mal seizures and circling. macroscopic, histological and immunohistochemical studies revealed acute systemic cdv infection ...19902385981
transforming genes of canine adenovirus type 2.genes of canine adenovirus type 2 (cad2) involved in transformation were localized and their functions investigated. cells transformed by cad2 whole dna or the bamhi a fragment (leftmost 47%) totally lacked contact inhibition and were serum-independent, anchorage-independent and tumorigenic in newborn rats. all the cells transformed by the xbai d (0 to 15.2%) and the ecori c (0 to 11.3%) fragments were morphologically transformed, but were serum-dependent, anchorage-dependent and not tumorigenic ...19882459301
characterization of a canine adenovirus hemagglutinin. 19714323345
effects of vaccines on the canine immune system.the effects of several commercially available polyvalent canine vaccines on the immune system of the dog were examined. the results demonstrated that the polyvalent vaccines used in this study significantly suppressed the absolute lymphocyte count and that most of the polyvalent vaccines significantly suppressed lymphocyte response to mitogen, but had no effect on natural effector cell activity, neutrophil chemiluminescence, nor antibody response to canine distemper virus. the individual vaccine ...19892540897
molecular cloning and restriction endonuclease mapping of two strains of canine adenovirus type 2.the dna of a field isolate and of a vaccine strain of canine adenovirus type 2 (cav-2) were analysed by digestion with several restriction endonucleases. the psti restriction fragments of the field isolate (cav-2 glasgow) and the vaccine strain were cloned into the plasmid pbr322. physical maps of the two viral genomes were constructed by molecular hybridization of psti, ecori, smai, bamhi and kpni digests of the viral dna with the cloned psti fragments. the restriction profile of cav-2 glasgow ...19892732685
canine adenovirus nephritis. 19714335376
oral vaccination of dogs fed canine adenovirus in baits.six groups of 5 dogs each were fed dilutions of canine adenovirus-2, either as raw liquid or after insertion into cornmeal baits. by the fourth week after vaccination, 29 of the 30 dogs developed high titers of serum-neutralizing antibodies to the virus.19892764336
canine adenovirus in organ cultures. 19724338601
the genetic relationship of two canine adenoviruses as determined by nucleic acid hybridization. 19724343898
incomplete particles of canine adenovirus type 1: occurrence and structure.replication of canine adenovirus (cav) type 1 in dog kidney (dk) cells has been found to generate particles containing double-stranded dna molecules of size 6-9 kbp with protein moieties at their 5' termini. the particles formed a discrete band in cscl gradients at a density intermediate to that of intact virions and empty capsids. electron microscopy demonstrated that they were morphologically similar to intact virions. restriction enzyme digests and hybridization experiments revealed the exist ...19892815582
characterization of the genome of a vaccine strain of canine adenovirus type 1.restriction endonuclease cleavage maps have been constructed for the genome of a canine adenovirus type 1 (cav-1) vaccine strain (cll; connaught laboratories, ltd., willowdale, ontario). restriction enzyme analyses were also carried out on cav-1 (cll) genomes isolated from viral stocks over 8 serial passages in a dog kidney cell line (dk 6722). the right hand 20% of the genome became more heterogeneous in size with increasing passage in dk 6722 cells due to deletions up to 3-4 kb, whereas the le ...19882852417
occurrence of canine adenovirus antibodies and non-specific inhibitors of haemagglutination in the sera of diverse animal species. 19734357493
mesangial localization of immune complexes in experimental canine adenovirus glomerulonephritis. 19744375485
bronchiolitis obliterans and pneumonia induced in young dogs by experimental adenovirus infection.young beagle dogs were experimentally inoculated with canine adenovirus type 2 and studied by virologic, histologic, immunoperoxidase, and ultrastructural methods from 1 to 26 days after inoculation. virus was recovered from lungs at 2, 3, 5, and 8 days after inoculation. virions and viral antigen were demonstrated by ultrastructural and immunoperoxidase techniques in nonciliated bronchiolar epithelial cells and mucous cells in bronchioles, bronchi, and trachea as well as in bronchial and trache ...19852990219
two distinctive transforming dna regions on the canine adenovirus type 1 genome.by transfecting cloned dna fragments of canine adenovirus type 1, a novel transforming region of mammalian adenovirus was demonstrated inside the viral genome. besides the left-end smai-d fragment (left 17.2%), an internally located smai-e fragment (17.2-28.7%) of canine adenovirus type 1 was found to transform primary rat kidney cells.19852991059
serologic evaluation of vaccinated american river otters.the oklahoma department of wildlife conservation acquired 20 american river otters (lutra canadensis) between 1984 and 1985 for reintroduction into oklahoma waterways. in 1985, 10 otters were evaluated for serum antibody titers after vaccination with canine distemper virus, canine adenovirus type 2, canine parvovirus (cpv), feline panleukopenia virus (fpv), feline rhinotracheitis virus (frv), and feline calicivirus. prevaccination serum-virus neutralization (svn) antibody to feline rhinotracheit ...19853001000
morphological studies of a canine adenovirus. 19684868889
comparative analysis of the canav-1 and canav-2 genomes.the molecular weights of canine adenovirus type 1 (canav-1, strain utrecht) and type 2 (canav-2, strain toronto a26/61) dnas were determined by contour length measurements and restriction endonuclease analysis. in each case, an average molecular weight value of 20 (+/- 0.5) x 10(6) daltons was obtained with both methods. similar in size, the canav-1 and canav-2 dna molecules were, however, cleaved at very distinct positions by a variety of restriction endonucleases. the observed lack of dna sequ ...19863032842
multiple reiteration of a 40-bp nucleotide sequence in the inverted terminal repeat of the genome of a canine adenovirus.the dna of a vaccine strain of canine adenovirus type 1 [ichv vaccine; connaught laboratories, ltd.; cav-1(cll)] has been cloned in plasmid pat153 in the form of subgenomic bamhi digestion fragments. analysis of the nucleotide sequences of cloned terminal fragments has revealed an inverted terminal repeat (itr) with a minimum length of 198 nucleotides, including a tandem reiteration of the 40-bp nucleotide sequence from positions 14 to 53. the itrs had the 5'-catcatcaat ... sequence typical of a ...19873037784
serum antibody response to canine parvovirus, canine adenovirus-1, and canine distemper virus in dogs with known status of immunization: study of dogs in sweden.serum antibody titers to canine parvovirus (cpv), canine adenovirus-1 (cav-1), and canine distemper virus (cdv) were measured in dogs with known immunization status. the dogs represented 3 groups: nonvaccinated dogs less than 12 months old; vaccinated dogs less than 12 months old; and adult dogs greater than 12 months old. for practical reasons, the population from which the specimens were obtained could be considered as free from natural infection with cav-1 and cdv. in nonvaccinated dogs less ...19883223653
a study of the pathogenicity of a canine adenovirus (toronto a26-61) for dogs. 19695815925
cloning, physical mapping and cross-hybridization of the canine adenovirus types 1 and 2 genomes.cloned hindiii fragments of the type 1 and type 2 canine adenovirus (canad) genomes were mapped with respect to the bamhi, ecori, hincii, paer7 and psti restriction endonuclease cleavage sites. considering the large differences found between the canad-1 and canad-2 dna molecules, in terms of number and location of restriction sites, cross-hybridization experiments were performed. homologous dna sequences were located on the canad-1 and canad-2 physical maps. both viruses are genetically related ...19873440519
tumors induced in hamsters by a respiratory-associated canine adenovirus (a26-61). 19704315946
the effect of temperature on the hemagglutinin activity of the canine adenovirus (infectious canine hepatitis virus). 19704326627
genotypic characterization of type-2 variants of canine adenovirus.two canine adenovirus (cav) isolates, apparently distinct from type-1 (utrecht) and type-2 (toronto a26/61) reference strains in their biochemical and/or immunologic properties, were submitted to dna-restriction endonuclease analysis. both isolates, designated iaf-81-2116 and iaf-75-95, appeared as genotypic variants of cav-2. isolate iaf-81-2116 was recovered from the intestine of a young pup with diarrheal disease. seemingly, relatively small changes in the original cav-2 dna sequence allowed ...19863963563
oral administration of an attenuated strain of canine adenovirus (type 2) to raccoons, foxes, skunk, and mongoose.an attenuated strain of canine adenovirus type-2 (cav-2) was administered orally to 2 foxes (vulpes fulva), 6 raccoons (procyon lotor), a skunk (mephitis mephitis), and a mongoose (herpestus auropunctatus). blood was collected weekly from the animals to monitor cav-2 virus-neutralizing antibody titers. all animals had increases in titers. sera from 8 foxes, 30 mongooses, 52 raccoons, and 22 skunks trapped in the field had naturally occurring antibody to cav-2.19883348527
serologic study of a canine adenovirus (toronto a26/61) infection in dogs. 19694307342
comparison of the immunological properties of two canine adenoviruses. 19724338316
isolation of canine adenovirus-2 from the faeces of dogs with enteric disease and its unambiguous typing by restriction endonuclease mapping.fifty-four faecal specimens obtained from kennelled dogs with diarrhoeal disease were used to inoculate a range of cell types in tissue culture. particles resembling adenovirus virions were seen in three specimens and 22 stools yielded an adenovirus upon culture. viral dna from each isolate was digested with the restriction endonucleases bam h1 and pst 1. agarose gel electrophoresis revealed identical restriction patterns for all isolates. one isolate, 9228, was selected as a prototype and was c ...19882836923
abortive infection of human cell cultures by a canine adenovirus. 19694306056
should the criteria for species distinction in adenoviruses be reconsidered? evidence from canine adenoviruses 1 and 2.four strains of canine adenovirus type 1 (cav-1) and two strains of canine adenovirus type 2 (cav-2) were examined by restriction enzyme analysis. in all cases, cav-1 could be readily differentiated from cav-2. additionally, monoclonal antibodies were prepared against the mirandola and manhattan strains of cav-1 and cav-2, respectively. 18 of 36 monoclonal antibodies from the cav-1 fusion and 77 of 160 monoclonal antibodies from the cav-2 fusion were type-specific by an indirect fluorescent-anti ...19853884549
[diagnostic problems posed by respiratory infections of dogs].the following viruses as well as bacteria and mycoplasma have been isolated from dogs with contagious respiratory disease: canine distemper virus; canine adenoviruses (type 1 and 2); parainfluenza type 2 (sv5); reovirus type 1; canine herpesvirus; bordetella bronchiseptica, streptococcus, pasteurella, staphylococcus and mycoplasma. the occurrence of these agents can be in direct relationship with: the evolution of a systemic disease; respiratory disorders being a regular or inconsistant symptom ...19852998696
association of a type-2 canine adenovirus with an outbreak of diarrhoeal disease among a large dog congregation. 19852999217
immunohistochemical detection of canine adenovirus in paraffin sections of liver.an avidin-biotin immunoperoxidase procedure was optimized for detection of canine adenoviral antigens in formalin-fixed, paraffin-embedded liver. long-term stability of viral antigen was shown by successful demonstration of virus in liver tissue preserved up to six years from dogs with infectious canine hepatitis. this immunohistochemical stain was applied to sections from livers with a wide range of inflammatory lesions. examination of sections from 53 dogs yielded five livers with small amount ...19863018984
monoclonal antibodies to canine adenoviruses 1 and 2 that are type-specific by virus neutralization and immunofluorescence.monoclonal antibodies were produced against the mirandola strain of canine adenovirus type 1 (cav-1) and the manhattan strain of canine adenovirus type 2 (cav-2). the monoclonal antibodies were used in vitro in virus neutralization (vn) assays and in indirect fluorescent antibody (ifa) tests to examine several strains of each virus type. out of 36 monoclonal antibodies produced against the mirandola strain, 18 were type-specific for cav-1 by ifa and 13 of those neutralized the virus in vitro. th ...19883281371
canine adenovirus respiratory disease: isolation of infectious canine hepatitis virus from natural cases and the experimental production of the disease. 19724338654
canine adenovirus-induced ocular lesions in the afghan hound.a series of referred cases of cav-1-induced ocular disease suggested increased susceptibility of the afghan hound. of a litter of afghan hound puppies inoculated with a commercial vaccine containing attenuated cav-1, three developed corneal edema and the litter as a whole showed marked febrile and serological responses to the virus. under experimental conditions afghan hounds inoculated with cav-1 at two levels of attenuation showed more profound clinical responses than beagles receiving similar ...19816262015
experimental canine adenovirus glomerulonephritis: persistence of glomerular lesions after oral challenge.ten dogs were given canine adenovirus by the non-lethal oral route. eight dogs were biopsied at 10 days after administration of virus in order to establish the presence of immune-complex glomerulonephritis. at this time, granular deposits of igg, igm, c3 and viral antigen were observed in a mesangial location and electrondense deposits were found. the dogs were then killed up to 40 days after receiving virus and, in all cases, deposits of immunoglobulins and complement together with the mesangia ...19816263307
biophysical comparison of two canine adenoviruses.the two canine adenoviruses, infectious canine hepatitis (ich) virus and infectious canine laryngotracheitis (icl) virus (also designated as toronto a26/61 virus), were studied with respect to their morphology and the biological properties of their soluble components. the two viruses were found to be composed of soluble components similar to, and carrying biological activities corresponding to, those of the human adenoviruses after fractionation by rate zonal centrifugation and anion-exchange ch ...19704318090
intranasal vaccine trial for canine infectious tracheobronchitis (kennel cough).two field trials were conducted during periods of endemic (summer) and epizootic (winter) canine infectious tracheobronchitis activity to evaluate the efficacy of three intranasal vaccines in a closed commercial beagle breeding kennel. a trivalent vaccine containing bordetella bronchiseptica, canine parainfluenza, and canine adenovirus-2 was administered at 3 weeks of age. the vaccine was 71.2% and 81.8% effective in decreasing the incidence of coughing during the winter and summer trials, respe ...19816273648
pathology of natural canine adenovirus pneumonia. 19816275478
immunoperoxidase study of canine distemper virus pneumonia.forty-two cases of canine pneumonia were examined for the presence of canine distemper virus. for that purpose canine distemper virus inclusion bodies were located. the histopathological lesions were related to the presence of canine distemper antigen, as demonstrated with an immunoperoxidase technique. this technique was more sensitive for detecting canine distemper infection in lung tissue than was the study of inclusion bodies. attention was also paid to combined infection with canine adenovi ...19836304834
corneal oedema after vaccination of dogs with canine adenovirus 2. 19836651694
identification of a canine adenovirus (infectious canine hepatitis virus) inhibitor in dog liver extracts as arginase.extracts of canine liver inhibited growth of infectious canine hepatitis (ich) virus, a canine adenovirus. purified extracts from mammalian, but not avian, liver tissue contained the inhibitor, and evidence is presented that the inhibitory factor is the enzyme arginase (arginine ureohydrolase). this study further emphasized the need for arginine in adenovirus growth and may explain some of the difficulties in isolating small amounts of ich virus from suspensions of liver.19724344396
serologic survey of giant pandas (ailuropoda melanoleuca), and domestic dogs and cats in the wolong reserve, china.sera from captive and recently rescued giant pandas (ailuropoda melanoleuca) in the wolong reserve, china, were examined by serum neutralization or hemagglutination inhibition for antibodies to canine distemper virus (cdv), canine coronavirus (ccv), canine herpesvirus (chv), pseudorabies virus (prv), canine adenovirus type 2 (cav), and canine parvovirus (cpv). serum samples from village domestic dogs and cats, which run free throughout the reserve also were examined. antibodies against cpv were ...19948151830
pathogenicity of low-virulence strains of two canine adenovirus types. 19734348389
the 'blue eye' phenomenon.diffuse clouding of the cornea ('corneal oedema', 'blue eye') of sudden onset and usually transient duration and with accompanying anterior uveitis, may be attributable to natural infection with canine adenovirus type 1 (ca-1) or to vaccination with live modified virus. it is now recognised that kerato-uveitis is a manifestation of type iii hypersensitivity in which immune complex formation resulting from the release of virus, especially from infected corneal endothelial cells, brings about corn ...19836304988
ultrastructure of the kidney and urinary excretion of renal antigens in experimental canine adenovirus infection. 19734375839
experimental canine adenovirus glomerulonephritis: histological, immunofluorescence and ultrastructural features of the early glomerular changes.eighteen 16-week-old dogs were inoculated i.v. with a virulent strain of canine adenovirus and the renal glomeruli were examined by light, electron (transmission and scanning) and immunofluorescence microscopy at intervals from 24 h to 7 days after inoculation of virus. from 2 days onwards intranuclear inclusion bodies and cell-associated viral antigen were detected in mesangial, endothelial and circulating mononuclear cells. this was accompanied by swelling and, in some cases, necrosis of these ...19836309209
macromolecular content of inclusions produced by a canine adenovirus.early inclusions induced by a canine adenovirus in a canine cell line, appearing before the formation of infectious virus particles, were purified by differential centrifugation in sucrose followed by cscl density gradient centrifugation. chemical analysis of these inclusions revealed that they contained deoxyribonucleic acid (dna), ribonucleic acid, and protein. on the basis of density gradient centrifugation, the dna extracted from the inclusions was found to be viral dna. electron microscope ...19724673491
immunity to canine adenovirus respiratory disease: effect of vaccination with an inactivated vaccine.nine puppies without maternal antibody to canine adenovirus (cav) were divided into two groups. the first consisted of six puppies, each of which was given two doses of a commercial inactivated cav-1 vaccine, 14 days apart. eight days after administration of the second dose of vaccine, all six puppies, together with the second group, consisting of three unvaccinated controls, were challenged with an aerosol of virulent cav-2. one dog from each group was killed on the third, fifth and 10th days a ...19836318419
serologic survey for selected viral pathogens in brown bears from italy.blood samples were collected from six captive bears and nine free-ranging marsican brown bears (ursus arctos marsicanus) in the abruzzo national park, italy, between 1991 and 1995. sera were tested for evidence of exposure to canine distemper virus (cdv), canine adenovirus type 2, canine coronavirus, and canine parvovirus type 2 (cpv-2). serologic evidence of cdv and cpv-2-exposure was found in both captive and free-ranging bears. this may be the first report of cdv exposure in free-ranging bear ...19979131563
urinary tract infection and pyelonephritis due to escherichia coli in dogs infected with canine adenovirus. 19744365947
canine viruses and multiple sclerosis.serum and cerebrospinal fluid from multiple sclerosis (ms) patients and control subjects were tested and compared for presence and titer of neutralizing antibody against the most common canine viruses. canine viruses included canine distemper virus (cdv), canine adenovirus 1 (cav-1), canine parainfluenza virus (cpiv), canine herpesvirus (chv), canine coronavirus (ccv), and canine parvovirus (cpv). neutralizing titers against measles virus (mv) and human adenovirus 8 (ha8) were also tested. signi ...19816267515
diagnosis of rotavirus, adenovirus, and herpesvirus infections by immune electron microscopy using a serum-in-agar diffusion method.the sensitivity of immune electron microscopy (iem) for the detection and identification of bovine rotavirus, infectious bovine rhinotracheitis virus (ibr), and canine adenovirus has been studied by using the serum-in-agar (sia) method in which a specific antiserum has been incorporated in agar.19806250692
establishment of a canine cell line: derivation, characterization, and viral spectrum.a cell line, designated a-72, for virus studies was established from a tumor surgically removed from a female, 8-year-old golden retriever dog. following explant culture, the cells were serially passaged 135 times. the a-72 cells maintained a fibroblastic appearance and, at the 123rd passage, had a population doubling time of approximately 27 hours. karyotypic analysis of the high passage cells showed the modal 2n chromosome number was 92 to 93. using starch gel electrophoresis for enzyme charac ...19806254399
kennel cough and canine adenovirus vaccines in australia. 19846497798
isolation of canine adenovirus a26-61 (toronto) using canine kidney (m.d.c.k.) cell line. 19734763774
seroprevalence of selected disease agents from free-ranging black bears in florida.sera obtained from 66 free-ranging florida black bears (ursus americanus floridanus) from three geographic areas of florida (usa) between november 1993 and august 1995 were tested for antibodies to 13 disease agents. antibody prevalences were 3 positive of 37 tested (8%) coxiella burnetti, 37 of 66 (56%) toxoplasma gondii, 3 of 61 (5%) bluetongue virus/epizootic hemorrhagic disease virus (btv/ehdv), 4 of 66 (6%) canine adenovirus-type 1, 5 of 66 (8%) canine distemper virus (cdv), 10 of 62 (16%) ...19989706572
immunity to canine adenovirus respiratory disease: a comparison of attenuated cav-1 and cav-2 vaccines.four litters of puppies were divided into three groups. one group was vaccinated with a live cav-1 vaccine and another with a live cav-2 vaccine. throat swabs were collected from two dogs in each of these groups to monitor the possible excretion of vaccine virus, but none was found. both groups, together with the third group of unvaccinated controls, were challenged 17 days later with an aerosol of virulent cav-2. one dog from each group was killed on the third, fourth, seventh, ninth, 11th and ...19826280370
dual adenovirus and distemper virus pneumonia in a dog.a case of simultaneous infection of the lungs of a dog with canine distemper virus (cdv) and canine adenovirus (cav) is described. the techniques employed are histological stains, immunoperoxidase technique, and transmission electron microscopy. two viruses are shown to infect the same cells. the significance of dual infection of the same cells is discussed in comparison with literature data on in vitro experiments.19826287699
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