| cross-protective immunity to canine adenovirus type 2 by canine adenovirus type 1 vaccination. | | 1978 | 216291 |
| comparative studies on the soluble proteins of adenovirus type 1. | the soluble proteins of adenovirus type 1 have been separated and purified. their antigenic characteristics were compared in different precipitation experiments performed in electric field. both two-dimension immune electrophoresis and rocket electrophoresis can successfully be applied for quick diagnostic purposes. quantitative determination of virus proteins is also feasible by rocket electrophoresis. the isoelectric point values of hexon, penton and fibre were pi 4.55, pi 4.69 and pi 7.07, re ... | 1977 | 602822 |
| comparative study of adenoviruses with monoclonal antibodies. | the obtainment of monoclonal antibodies for adenovirus species 4(ad4) is described. the specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and enzyme-linked immunosorbent assay (elisa), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus c), 4 (subgenus e), 7 and 16 (subgenus b) and 9 (subgenus d). two monoclonal antibodies species specific to adenovirus 4 (1ciii and 3diii) and one ... | 1992 | 1284894 |
| hexagonal crystalline arrays of adenovirus type 1 hexons. | separated, highly purified and concentrated adenovirus type 1 soluble hexon capsomers were crystallized by dialysis against 0.5 m acetate buffer. the crystallization process was followed electron microscopically. in the early phase of the crystallization, groups of a few hexons began to appear, then the two-dimensional crystal lattices grew gradually to a size of 1-2 micron. simultaneously three-dimensional crystals of tetrahedral and prismatic shapes developed. the hexons in the two-dimensional ... | 1979 | 539469 |
| replication of ovine adenoviruses. | the replication of ovine adenoviruses shows intertypic and intratypic variations, e.g. the extracellular and intracellular virus yield of ort/111, a strain related to bovine adenovirus type 2, reached its peak 40 to 46 hr postinfection, in contrast to gy/14, a strain classified as ovine adenovirus type 1, which required 52 to 58 hr to reach the highest yield. the replication cycle was not appreciably influenced either by rolling of the tube cultures or the age (between 0 and 7 days) of the suppo ... | 1978 | 382754 |
| adenovirus infection and cytopathic alterations of human cervical epithelial cells in vitro. | the effect of adenovirus infection was studied in monolayer cultures of human ecto- and endocervical epithelial cells. in both types of cultures adenovirus type 1 resulted in a slowly progressing lytic infection with persistent production of infectious virus, viral antigens and of cytopathic alterations in a fraction of cells. the changes were similar in ecto- and endocervical epithelial cells. although many of the alterations detected may represent unspecific lesions, nuclear changes consisting ... | 1978 | 282757 |
| [trial of a hyperimmune serum against para-influenza 3, bovine adenovirus 1, infectious rhinotracheitis and mucosal disease]. | a four-valent serum was obtained by parallel hyperimmunization of cattle with live viral suspensions of parainfluenza-3, bovine adenovirus i, the virus of infectious rhinotracheitis and the mucous disease virus. the serum's neutralizing titres are between 1:1280 and 1:2560. the lasting exploitation of serum producing cattle indicates that no competition exists between the four antigens used in the process of hyperimmunization. experimental testing of the serum on calves and its application in pr ... | 1979 | 231341 |
| [isolation of adenovirus type 1 from aborted cattle fetuses]. | five adenoviral strains, typed as adenovirus type 1, were isolated from aborted cattle foetuses, taken from farms with abortions in cows. the viruses were isolated from kidneys, lungs and livers of the foetuses. they were isolated from the kidney most frequenty (three strains). intranuclear basophilic inclusions in the alveolar epithelium of the lungs and in the tubular epithelium of the kidneys have been established in the histological investigations of the internal organs of the foetuses. the ... | 1978 | 223279 |
| in vivo expression of il-1 beta and il-6 genes during viral infections in human. | macrophage infiltration is a constant feature of human virus-infected tissues. however, the in situ functional status of these cells remains undetermined. in order to document an activation of macrophages in virus-infected tissues, the expression of il-1 beta and il-6 genes was analyzed using in situ hybridization. several tissues were studied, as well as infections induced by different viruses: lymph nodes infected by hiv-1 (9 cases) or ebv (one case), lungs infected by cmv (5 cases) or adenovi ... | 1991 | 1654144 |
| [enzymatic activity of the lymphocytes in gnotobionts in adenovirus infection]. | the cytochemical activity of succinate dehydrogenases, alpha-glycerophosphate (mitochondrial and hyaloplasmic), lactate, glycose-6-phosphate, glutamate, beta-oxibutyrate, nadp-h2- and nad-h2 diaphorases and acid phosphatase was studied in lymphocytes of gnotobiotic guinea-pigs infected with human adenovirus type 1. the analysis of transgression of distribution of the values in the experimental and control groups revealed a high informative diagnostic value of acid phosphatase and lactate dehydro ... | 1978 | 208307 |
| structural analysis of viral replicative intermediates isolated from adenovirus type 2-infected hela cell nuclei. | deoxyribonucleoprotein complexes released 17 h postinfection from adenovirus type 1 (ad2)-infected hela cell nuclei were shown by electron microscopy to contain filaments much thicker (about 200 a [20 nm]) than double-stranded dna (about 20 a [2 nm]). the complexes were partially purified through a linear sucrose gradient, concentrated, and further purified in a metrizamide gradient. the major protein present in the complexes was identified as the 72,000-dalton (72k), adenovirus-coded single-str ... | 1978 | 207893 |
| [testing the preventive properties of polyvalent inactivated vaccine (ibr, pi-3, adenovirus 1,3) at a cattle-breeding farm]. | tested was an inactivated polyvalent vaccine. it was shown to produce virus-neutralizing antibodies against the respiratory viruses in high titers. the vaccine proved highly immunogenic, reactogenic, and harmless in doses of 2,3,5,7, and 10 cm3 for 30-45-day-old calves and pregnant cows in their 7th or 8th month of pregnancy. the morbidity rate among the unvaccinated calves ranged from 51 to 66 per cent, and among the vaccinated ones it was from 0 to 5 per cent on the four farms studied. the abo ... | 1976 | 189483 |
| hemagglutinating properties of celo, an oncogenic avian adenovirus. | chicken-embryo-lethal-orphan (celo) virus, phelps strain, agglutinated erythrocytes at 37 c. the hemagglutinating activity, which is a function of complete and incomplete virus particles, was sensitive to heat but not to ph. the soluble components of the virus were similar in sedimentation characteristics to those obtained from human adenovirus type 1. the effects of chemical and physical agents on celo hemagglutinin, celo infectivity, and red-cell receptors suggested that the last were protein ... | 1975 | 173279 |
| canine adenovirus type 2-induced immunity to two canine adenoviruses in pups with maternal antibody. | twenty-four beagle pups with high levels of maternal antibody to canine adenovirus type 1 (cav-1) and canine adenovirus type 2 (cav-2) were oronasally inoculated with cav-2 at 4 weeks of age. the cav-2 was isolated from pharyngeal swabs on postinoculation days 2 through 6. in spite of the infection, maternal antibody continued to decrease for 4 to 8 postinoculation weeks, and then homologous cav-2 neutralizing antibody and, to a lesser extent, cav-1 neutralizing antibody began to increase. when ... | 1975 | 168797 |
| identification of atgcat sequence at sites of smuc i restriction endonuclease by computer and physical mapping of adenovirus type 1 dna. | physical mapping of adenovirus type 1 dna was carried out in order to analyze the recognition sequence of a novel streptococcus restriction endonuclease. in addition to the new map and homology data on this poorly analyzed serotype, the result offers the definite evidence for the atgcat recognition sequence on adenovirus dna and the physical map of cleavage points. | 1991 | 1810111 |
| [group and type antigenic specificity of bovine adenovirus type 1 hexons]. | hexones were obtained from the bovine serotypes 1 and 3. they were investigated through the complement-fixation test along with intact virions in the presence of a hemotype and a heterotype antiserum. it was shown that the free hexones were twice as reactogenic in the homotype system as compared to the heterotype one. the hexones were found to possess two antigenic determinants--a typespecific one and a group-specific one. the latter proved sensitive to chymotrypsin. intact vicions reacted chief ... | 1977 | 76368 |
| early region 4 sequence and biological comparison of two isolates of mouse adenovirus type 1. | the dna sequence of 88-100 map units of mouse adenovirus type 1 (mav-1) was determined. one translational open reading frame showed 48% sequence similarity to a human adenovirus type 2 early region 4 protein. based on the protein similarity, genome location, and transcriptional polarity, we concluded that this region of mav-1 corresponds to early region 4. a 241-bp sequence consisting of 10 imperfect direct repeats with sequence similarity to minisatellite dna was found in this region. two virus ... | 1991 | 1845825 |
| murine adenovirus infection of scid mice induces hepatic lesions that resemble human reye syndrome. | murine adenovirus type 1 (mav-1) infection of cb-17 scid mice (which are homozygous for the severe combined immunodeficiency mutation) induces hepatic histopathologic and ultrastructural features that are strikingly similar to human reye syndrome. gross pathologic examination of mav-1-infected mice revealed only pale yellow liver tissue. histopathologic studies of tissue from mav-1-infected mice revealed diffuse hepatic injury manifested by microvesicular fatty degenerative changes of hepatocyte ... | 1991 | 1852004 |
| differences in the e3 regions of the canine adenovirus type 1 and type 2. | sequence analysis of a region extending between the pviii and the fiber gene of canine adenovirus type 1 (cav-1, utrecht) and type 2 (cav-2, manhattan) was performed. the results revealed a high level of identity between the two viruses when the pviii gene and the n-terminal part of the fiber gene were compared. the open reading frames of region e3 in of cav-1 and cav-2 encoded a 22 kda and 40.7 kda polypeptide, respectively. the cav-1 and cav-2 e3 region polypeptides shared conserved amino and ... | 1992 | 1534956 |
| restriction enzyme maps for equine adenovirus 1 genome. | physical maps were constructed for the genome of equine adenovirus 1 (eav1) using the restriction enzymes; drai, ecorv, noti and sfii. the total size of the eav1 genome was 34.4 kb estimated by comparison with known dna standards and the polarity of the fragment order, with respect to the left and right molecular ends, was determined by hybridization with known regions of the human adenovirus 2 (hav2) genome. | 1992 | 1496807 |
| nucleotide and deduced amino acid sequence of the canine adenovirus type 1 proteinase. | the dna sequence of an open reading frame (orf) corresponding to the canine adenovirus type 1 (can1) proteinase gene was determined. a total of 1171 base pairs were sequenced from the downstream end of the hindiii-a can1 genomic fragment, including adjacent regions corresponding to the carboxy-terminal portions of the hexon and the dna-binding proteins. the predicted can1 proteinase consists of 206 residues (23,325 d) of which 68% are identical and 83% are similar to the sequence of the human ad ... | 1992 | 1413543 |
| lethal disseminated adenovirus type 1 infection in a patient with chronic lymphocytic leukemia. | a 45-year-old woman with chronic lymphocytic leukemia developed fever followed by decreasing liver function. her clinical condition rapidly deteriorated, acute liver necrosis developed, and the patient died of hepatic and renal failure. adenovirus type 1 was isolated before death from blood, urine and throat and found in liver and lung at autopsy. serologic studies showed that the infection was primary and that the patient was unable to mount an antibody response to the infection. adenovirus typ ... | 1990 | 2259869 |
| epidemiology of acute respiratory infections in young children from thailand. | a prospective evaluation of the epidemiology and presentations of acute respiratory infections in children younger than 5 years of age admitted to children's hospital bangkok (1988 to 1989) was supported by the world health organization. there were 226 patients with the inclusion criteria: 1 to 5 years of age; duration of illness less than 2 weeks; no prior antibiotic therapy; and low socioeconomic status. the disease categories included: croup, 19 cases; bronchiolitis, 60 cases; and pneumonia, ... | 1990 | 2277743 |
| sequence of mouse adenovirus type 1 dna encoding the amino terminus of protein iva2. | | 1990 | 2374728 |
| vaccine-associated canine distemper infection in a litter of african hunting dogs (lycaon pictus). | four, 57 days old, african hunting dog puppies (lycaon pictus) from one litter died within three weeks following vaccination with modified-live canine distemper virus (cdv) and killed canine adenovirus type 1, canine parvovirus and leptospira icterohemorrhagiae and canicola. 18 days post vaccination, the animals developed neurologic disease characterized by episodes of grand mal seizures and circling. macroscopic, histological and immunohistochemical studies revealed acute systemic cdv infection ... | 1990 | 2385981 |
| delineation of antigenic determinants of adenovirus hexons by means of monoclonal antibodies. | 60 hexon-specific mouse hybridoma clones were selected by using crystallized hexon capsomers of human adenovirus type 1 as immunizing and selecting antigen. the reactivities of the monoclonal antibodies were tested with purified hexon preparations from 10 human adenovirus types by enzyme-linked immunosorbent assay and passive hemagglutination techniques. the reactivity patterns delineated a total of 41 adenovirus-type-1-related determinants present on a number of heterologous hexons in different ... | 1985 | 2409054 |
| transcription mapping of mouse adenovirus type 1 early region 4. | early region 4 (e4) of mouse adenovirus type 1 was analyzed by northern blotting, cdna sequencing, and s1 nuclease protection and primer extension assays. the transcription map of this region was dissimilar to the consensus human adenovirus e4 transcription map in which all transcripts have identical 5' and 3'-terminal sequences. seven classes of mouse adenovirus type 1 mrnas were identified; all shared the same 3' end. three classes of unspliced mrnas differed at their 5' start sites, two class ... | 1992 | 1388309 |
| [the inhibiting action of superhigh-frequency millimeter waves on adenovirus (author's transl)]. | virological and biochemical analysis was employed for comparative studies of the content of protein in continuous cultures of human embryo kidney cells (rh line) infected with adenovirus type 1, intact and irradiated with electromagnetic waves of uhf in the range of 5 divided by 8 mm at the density of power current of the order of 4 mw. when the cells were inoculated with intact virus, a cytopatic effect, a decrease in the content of protein by 4 hours postinfection and further decrease up to 72 ... | 1975 | 1210320 |
| corneal endothelium in viral induced anterior uveitis. ultrastructural changes following canine adenovirus type 1 infection. | dogs inoculated intravenously with attenuated canine adenovirus type 1 developed anterior segment inflammation and corneal edema. during the stage of mild anterior uveitis, virus was isolated from the aquious fluid, and by electron microscopy, viral replication was found to occur in corneal endothelial cells. later, at the stage of severe anterior uveitis with corneal edema, virus was not isolated from the aqueous fluid and cells containing intranuclear (replicating) virus were not found. at thi ... | 1975 | 1138688 |
| viral-antibody complexes in canine adenovirus type 1 (cav-1)ocular lesions: leukocyte chemotaxis and enzyme release. | canine adenovirus-type 1 (cav-1)-antibody complexes caused severe anterior uveitis with corneal edema ("blue eye") when injected into the anterior chamber of normal dogs. the response of the anterior uvea to such immune complexes (ic) was similar to the spontaneously occurring disease. in the presence of complement (c'), ic caused release of neutrophile chemotactic factors. following phagocytosis of ic-c' leukocytes released lysosomal enzymes, as indicated by the presence of acid phosphatase in ... | 1975 | 1095299 |
| studies on the different properties of adenovirus penton. | the physical, chemical, immunological and biological properties of the soluble penton antigen of adenovirus strains types 1, 5, 7, 8 and 9, belonging to three distinct subgroups of human adenoviruses, were analysed. mono-, di-, and polymers of the penton were studied by complete and incomplete hemagglutination and a method was developed to separate pentons of subgroup ii. immunological techniques were developed for the rapid detection of penton antigen. elution characteristics of the pentons of ... | 1977 | 867802 |
| adenovirus type 2 coded single-stranded dna binding protein: in vivo phosphorylation and modification. | the adenovirus type 2-coded single-stranded dna binding protein (dbp) was shown to be a phosphoprotein and to exist in at least two forms that differ in mobility by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. after a 30-min pulse with [35s]methionine or 32po4, 35s- or 32p-labeled dbp had a nominal molecular weight of 74,000 whereas after a 30-min label followed by a 20-h chase, 35s- and 32p-labeled dbp had a nominal molecular weight of 77,000. both large and small forms of 35s- an ... | 1977 | 864833 |
| antigenic structure of mammalian adenoviruses studied by monoclonal antibodies against bovine adenovirus type 2. | the cross-reactivity pattern of eight monoclonal antibodies (mabs) raised against bovine adenovirus type 2 (bav2) hexon was studied by indirect elisa using seven different adenoviruses. all of the eight mabs reacted with the two bav2 subtypes (a and b), while the other adenoviruses, human adenovirus type 1 (hav1), ovine adenovirus type 3 (oav3), bav1, bav4 and bav8 gave different combinations of positive reactions. based on the results of cross-reactivity there is a close antigenic relationship ... | 1988 | 2469284 |
| [features of the interaction of human adenovirus type 1 process with erythrocytes]. | | 1986 | 2482434 |
| the use of agarose gels for the assay of the deoxyribonuclease activity associated with the soluble penton of adenoviruses. | evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected hep-2 cells also carry an endonucleolytic activity. the effect was measured with both e. coli and adenovirus type 1 double-stranded dna substrates. the use of agarose gels for assaying the endonuclease activity allowed the approximative calculation of the number of penton molecules which might produce one dna double-strand break under the experimental conditions selected. | 1976 | 797350 |
| kinetic studies on the cleavage of adenovirus dna by restriction endonuclease eco ri. | the kinetics of cleavage of dna from adenovirus type 1 (ad1), type 5 (ad5) and type 6 (ad6) by restriction endonuclease ecori was investigated by quantitative evaluation of the fluorescence from ethidium stained dna fragments separated on agarose gels. the apparent rate constants of cleavage at different cleavage sites have been determined and large differences in the cleavage rates of the individual sites within one type of dna were found. from the kinetics of cleavage information on the sequen ... | 1976 | 794836 |
| isolation of bovine adenovirus type 1 without an adenovirus-associated virus. | this report describes the isolation and identification of a strain of bovine adenovirus type 1 from a cow in a herd that had undergone an episode of diarrhea and abortions. this strain of bovine adenovirus type 1 did not agglutinate either guinea pig or human 0 erythrocytes, and no 20-nm virus particles were evident by electron microscopy, further indicating that it was not contaminated by adenovirus-associated virus. | 1978 | 749583 |
| an epidemic of adenovirus type 1 conjunctivitis. | | 1989 | 2560538 |
| identification and nucleotide sequence of the early region 1 from canine adenovirus types 1 and 2. | the genome of canine adenovirus type 1 (cav-1) has been cloned and restriction maps compiled. these maps are compared with those of canine adenovirus type 2 (cav-2). the left ends of both genomes were further characterised by dna sequence analysis. several features of the dna sequence and predicted polypeptide sequence are similar to those of the human adenoviruses. the level of homology observed across the e1 regions appears to be of the same order as the overall dna similarity between cav-1 an ... | 1989 | 2623943 |
| the oncogenicity of avian adenoviruses. iv. confirmatory evidence for recombination between viral and cellular dna sequences and repetition of the recombinant in cells of a tumor line. | on the basis of hybridization analyses with viral dna fragments as probes, we previously concluded that, in dnas of many fowl adenovirus type 1-induced rodent tumors, units consisting of viral dna segments flanked on both sides by cellular dna segments are repeated more than 100 times per diploid genome of cell. molecular cloning of virus-cellular junction fragments and analyses of their sequence arrangement in the present study confirmed that the previous conclusion was basically correct. | 1989 | 2705306 |
| [fragmentation of human adenovirus type 1 dna by the specific endonucleases r. sali, r. ecori and r. bamhi]. | the restricting endonuclease r. ecori hydrolyses human adenovirus type i (adl) dna with formation of three fragments with molecular weights of 17.5 x 10(6), 3.7 x 10(6), and 1.55 x 10(6) dalton. under the effect of r. sali restrictase dna adl disintegrates into 5 fragments with molecular weights: 8.0 x 10(6), 6.0 x 10(6), 4.6 x 10(6), 4.0 x 10(6), and 0.2 x 10(6) dalton. treatment of dna with r. bamhi produces 4 fragments with molecular weights 10.0 x 10(6), 6.8 x 10(6), 4.0 x 10(6), and 2.2 x 1 ... | 1978 | 749344 |
| [density gradient and ultrastructure of bovine adenovirus type 1]. | isolated and purified were virions and hexones from the bovine adenovirus type 1. the infectious virions were of equilibrium density of 1.34 g/cm3, and the empty capsids--of 1.305 g/cm3. the virions had a facet length of 43.5 nm, their size being 82.7 nm (distance between opposite poles) to 74.0 nm (diameter of the virion). the fibers of the bovine adenovirus type 1 measured 26.0--28.0 nm. the virus was destroyed by 30 per cent formamide, yielding free hexones of 1.290 g/cm3 of equlibrium densit ... | 1978 | 741648 |
| primary structure of the murine adenovirus type 1 proteinase. | the dna sequence of an open reading frame (orf) corresponding to the murine adenovirus type 1 (mav1) proteinase gene was determined. 1162 base pairs were sequenced from the downstream end of the smai-d mav1 genomic fragment. the sequence defines the 204 amino acid proteinase, which apparently does not possess the usual l3 polyadenylation signal, but instead the sequence aaataa. this gene is followed by a 147 amino acid c-terminal portion of the dna-binding protein, encoded by the complementary s ... | 1992 | 1536888 |
| prerequisites for oral immunization of free-ranging raccoons (procyon lotor) with a recombinant rabies virus vaccine: study site ecology and bait system development. | a model baiting system suitable for the delivery of an oral rabies vaccine to free-ranging raccoons (procyon lotor) was developed and tested on barrier islands in south carolina (usa). features of barrier island physiography and ecology were studied relative to selective bait deployment and site biosecurity. capture-mark-recapture data were obtained from 228 raccoons. raccoon density estimates, using a modified census assessment technique, were one raccoon per 1.8 to 2.7 ha. mean (+/- se) and ra ... | 1992 | 1548804 |
| [virologico-serologic studies in horses with respiratory tract diseases]. | of 1081 acute and chronically respiratory diseased as well as clinically normal horses 824 sera and 257 paired serum samples collected 1986 and 1987 were tested for antibodies against several different respiratory viruses such as influenza virus a/equi 1 and 2 (influenza 1 a. 2), equine herpesvirus type 1/4 (ehv 1/4), mammalian reovirus type 1-3 (reovirus 1-3), equine rhinovirus type 1 (erv 1), equine adenovirus type 1 (eadv 1), and equine arteritis virus (eav). the investigations resulted in an ... | 1992 | 1558530 |
| cell mediated immune response of chicks following fowl adenovirus type-1 infection. | cellular immune response of 6-week-old chickens infected with fowl adenovirus type-1 was evaluated by both in vivo and in vitro assay. in vitro assay, enumerating peripheral t lymphocyte by alphanaphthyl acetate esterase staining, revealed that cellular immunity appeared as early as 1 week post-infection and was maintained up to 5 weeks post-infection. in vivo assay by phytohaemagglutin skin test showed cellular immunity until 2 weeks post-infection. thereafter no immunity was observed by this a ... | 1991 | 1647932 |
| two distinctive transforming dna regions on the canine adenovirus type 1 genome. | by transfecting cloned dna fragments of canine adenovirus type 1, a novel transforming region of mammalian adenovirus was demonstrated inside the viral genome. besides the left-end smai-d fragment (left 17.2%), an internally located smai-e fragment (17.2-28.7%) of canine adenovirus type 1 was found to transform primary rat kidney cells. | 1985 | 2991059 |
| evaluation of mixed cell types and 5-iodo-2'-deoxyuridine treatment upon plaque assay titers of human enteric viruses. | four continuous cell lines, bgm, l-132, hel-299, and rd, were compared both when cultured separately and as mixtures for use in plaque assay titrations of human adenovirus 1 and six human enterovirus serotypes. the effect of incubating these cell cultures in media containing 5-iodo-2'deoxyuridine (idu) prior to inoculation with virus was also studied. the use of mixed-cell cultures revealed cell line-dependent synergistic effects as well as inhibitory effects. these effects were strongly virus d ... | 1986 | 3015021 |
| antibody response to modified-live canine adenovirus vaccine in african hunting dogs (lycaon pictus). | nine hunting dogs were bled pre and post vaccination with modified-live virus vaccine. their antibody responses to canine adenovirus type 1 was measured by an indirect immunofluorescent antibody test. the vaccine proved to be both effective and safe. | 1991 | 1659069 |
| epidemiology of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children in the tokyo area. | 82/2,223 stool specimens, collected 1982-1988, from children with enteritis (3.7%) were found to contain adenoviruses; 17 adenovirus-positive samples were provided from other institutes. 89 adenoviruses were isolated in graham 293 cells from these 99 specimens and were typed by dna restriction enzyme analysis with sma i. 37 strains were typed as adenovirus 40 (ad40), and 37 strains as adenovirus 41 (ad41). although most strains had the same dna profiles, a few strains had 3 kinds of different el ... | 1991 | 1662830 |
| direct virus inactivation of tachyplesin i and its isopeptides from horseshoe crab hemocytes. | direct virus inactivation of tachyplesin i and related isopeptides, which are antimicrobial peptides isolated from the hemocytes of the horseshoe crab (tachypleus tridentatus and limulus polyphemus), was examined against several viruses. vesicular stomatitis virus (vsv) was inactivated by incubation with tachyplesin i and its isopeptides. influenza a (h1n1) virus was slightly inactivated by tachyplesin i, whereas herpes simplex virus 1 and 2, adenovirus 1, reovirus 2 and poliovirus 1 were resist ... | 1991 | 1666545 |
| outbreak of adenoviral infections in a long-term paediatric facility, new jersey, 1986/87. | during an 8-week period in the winter of 1986-87, there were 11 deaths from an adenovirus infection (case fatality rate = 39%, 11/28) in a long-term care paediatric facility in southern new jersey. among the 61 resident children, all with severe congenital and/or acquired disabilities, 28 developed a febrile respiratory illness compatible with adenovirus infection [attack rate (ar) = 46%]. patients with tracheostomies were three times as likely to become ill [relative risk (rr) = 3.2, 95% confid ... | 1991 | 1680902 |
| identification of mouse adenovirus type 1 early region 1: dna sequence and a conserved transactivating function. | the left end of the genome of mouse adenovirus type 1 (also known as strain fl) was characterized by determination of the dna sequence, amino acid similarities with early region proteins of primate adenoviruses, and a functional assay. several specific dna sequence features were similar to those found in human adenoviruses, and open reading frames from this region could encode proteins similar to human adenovirus early region 1a and early region 1b proteins. dnas from this region were tested in ... | 1988 | 3172335 |
| serum antibody response to canine parvovirus, canine adenovirus-1, and canine distemper virus in dogs with known status of immunization: study of dogs in sweden. | serum antibody titers to canine parvovirus (cpv), canine adenovirus-1 (cav-1), and canine distemper virus (cdv) were measured in dogs with known immunization status. the dogs represented 3 groups: nonvaccinated dogs less than 12 months old; vaccinated dogs less than 12 months old; and adult dogs greater than 12 months old. for practical reasons, the population from which the specimens were obtained could be considered as free from natural infection with cav-1 and cdv. in nonvaccinated dogs less ... | 1988 | 3223653 |
| viral gastro-enteritis in children in malawi. | in a 2-month survey of 186 children with gastro-enteritis attending an out-patient clinic in malawi, 42% were infected with rotavirus (hrv), 9% cryptosporidium, 4.2% adenovirus, 1.2% astrovirus and 0.6% norwalk agent and small round featureless viruses. we believe this to be the first report of hrv in malawi and the first of astrovirus in africa. almost all the hrv infections were in children under 12 months old, 40% were in children under 6 months and 64% of children were being breastfed at pre ... | 1990 | 1694637 |
| sequence analysis of putative e3 and fiber genomic regions of two strains of canine adenovirus type 1. | the nucleotide sequences of the genomes of two strains of canine adenovirus, type 1 (glaxo and cll) have been determined within the region spanning approximately 0.80-0.91 map unit. homology comparisons with other adenoviral dnas indicate that this region contains part of the hexon-associated protein (pviii) precursor gene on the left, and a 57-kda fiber-encoding gene on the right. in both viral strains, the sequences of these genes are identical. accepting the generally highly conserved nature ... | 1991 | 1828920 |
| [mutagenic effect of human adenovirus type i on the somatic and sex cells of male mice]. | human adenovirus 1 was studied for its effect on the chromosomal apparatus both in bone marrow cells and male sex cells of mice. chromosome aberrations were most early detected in spermatocytes of the 1st order mice infected with human adenovirus 1. in bone marrow cells of mice the highest level of chromosome aberrations was observed 30, 60, 90 days after the inoculation, which corresponds to a more frequent detection of the adenoviral antigen. the uv-irradiated-virus caused chromosome aberratio ... | 1986 | 3705168 |
| [purification and properties of the hexon antigen of canine adenovirus serotype cav-1]. | the main antigen and immunogen of canine adenovirus type 1 (cav-1) has been purified to near homogeneity from cultural fluid of a cav-1-infected primary cell culture by hydrophobic and anion-exchange chromatography. the hexon native form (trimer) was shown to be resistant against denaturation by sds under conditions of sds-page performed without heating the samples. the monomer chain of the cav-1 hexon was apparently identical in terms of electrophoretic mobility with that of the previously sequ ... | 1990 | 2085617 |
| transcription mapping of mouse adenovirus type 1 early region 3. | early region 3 (e3) of mouse adenovirus type 1 was analyzed using s1 nuclease protection and primer extension assays, cdna sequencing, and genomic sequencing. we present the genomic sequence from 79 to 83 map units of the viral genome, the precise ends and splice sites of the e3 mrnas, and the predicted protein sequence encoded by the mrnas. three major classes of early mrnas were identified; all were approximately 1 kb long, consisted of three exons, and shared 5' and 3' ends. the three classes ... | 1990 | 2137954 |
| reactivity of mouse ascitic fluids containing monoclonal antibodies directed against adenovirus hexon. | thirty-two mouse hybridoma ascites were produced, containing hexon specific monoclonal antibodies, using crystallized hexons of human adenovirus type 1 as immunizing and selecting antigen. the reactivity of the ascitic fluids was tested with hexon preparations of 11 different human adenovirus species (serological types) belonging to 4 different subgenera by elisa and passive haemagglutination (ha) methods. by these methods the 32 hybridoma antibodies showed 4 and 12 distinct reactivity patterns ... | 1985 | 2412388 |
| isolation of bovine adenovirus type 1 in the sudan. | | 1973 | 4213204 |
| the presence of adenovirus-specific antigens in hamster cells rendered neoplastic by adenovirus 1-sv40 and adenovirus 2-sv40 hybrid viruses. | | 1967 | 4292152 |
| [experimental infections of cattle with human adenovirus (type 1)]. | | 1966 | 4293812 |
| rescue of heat-inactivated adenovirus type 1 and 6 by ultraviolet-irradiated adenovirus type 8. | | 1970 | 4316777 |
| isolation of bovine adenovirus type 1 from a calf with pneumo-enteritis. | | 1971 | 4333343 |
| [study on adenovirus type 1 infection. 1. virological and serological studies on adenovirus type 1 infection in infant asylum (author's transl)]. | | 1973 | 4363430 |
| [genetic markers of clonal variants of adenovirus type 1]. | | 1973 | 4365040 |
| [study on adenovirus type 1 infection: 1. virological and serological studies on adenovirus type 1 infection in infant asylum (author's transl)]. | | 1973 | 4377476 |
| nutrition and the growth of human adenovirus type 1 in monkey cell cultures: the importance of arginine. | | 1969 | 4387636 |
| differentiation of adenovirus hexon epitopes with monoclonal antibodies by gel diffusion assays. | nine monoclonal antibodies (mabs) directed against crystallized human adenovirus type 1 (ad h 1) hexon were tested with purified homologous and heterologous hexon preparations by gel diffusion. six mabs formed a single line with the homologous hexon in a 2-well pattern, and 3 mabs formed lines only in biclonal combinations with an appropriate mab. all of the 6 precipitating mabs formed a continuous line of complete identity when tested simultaneously against homologous and different heterologous ... | 1985 | 2413350 |
| grouping of monoclonal antibodies to adenovirus hexons by their cross-reactivity. | thirty two mouse ascitic fluids containing monoclonal antibodies (mabs) directed against the hexon of human adenovirus type 1 were examined. the type and degree of cross-reactivity (cr) of the mabs were determined by elisa and hemagglutination methods with 10 heterologous hexon types. the similarity and the dissimilarity of the mabs was also characterized by the correlation coefficient calculated from their reactivity values. on the basis of these, the 32 mabs could be divided into five groups a ... | 1986 | 2417575 |
| transformation of rat embryo cells by adenovirus type 1. | | 1969 | 4887234 |
| [stability of the structure and antigenic determinants of adenovirus type 1 native hexon to proteases]. | hexon capsomers of human adenovirus type 1 (h1) labeled by iodine 125 were digested in a native state (trimers) by trypsin, chymotrypsin or papain, and the resulting hydrolysates were analyzed by sds-page. in each case, a discrete and temporally stable pattern of relatively large fragments was revealed. the degree of hexon polypeptide hydrolysis was maximal for papain, intermediate for chymotrypsin and minimal for trypsin, the largest fragments in the digest being 32, 40 and 80 kd, respectively. ... | 1986 | 2429709 |
| multiple copies of identical epitopes on the adenovirus hexon. | a double monoclonal antibody (mab) sandwich enzyme-linked immunosorbent assay (double mab elisa), which uses the same mab as solid-phase immunosorbent (capture mab) and as detector mab (peroxidase-labeled), was developed to quantify the specific epitopes of adenovirus hexon. four mabs directed against crystallized adenovirus type 1 (ad h 1) hexon were tested by this assay with homologous and different heterologous hexons. the lowest reacting concn with the homologous and heterologous hexon types ... | 1986 | 2432411 |
| distribution pattern of adenovirus hexon epitopes in infected cells determined with monoclonal antibodies by immunofluorescence analysis. | two monoclonal antibodies (mabs) specific for two distinct epitopes on the human adenovirus type 1 (av1) hexon were used to determine the subcellular localization of hexon epitopes in the infected hep-2 cells by indirect immunofluorescence. on the basis of cross-reactivity pattern of mabs, presumably one of the epitopes is genus specific and the other should be intertype specific. the epitopes, i.e. the adenovirus hexons could be detected throughout the cell and could display different accumulat ... | 1987 | 2452546 |
| atypical adenovirus type 1 strains isolated from patients with laryngitis. | | 1969 | 5351213 |
| haemagglutinating adeno-associated virus (aav) in association with bovine adenovirus type 1. brief report. | | 1970 | 5530958 |
| the mouse adenovirus type 1 contains an unusual e3 region. | since the e3 region of human adenoviruses codes for a series of proteins that are probably involved in viral pathogenesis, the nucleotide sequence for a 3.6-kilobase dna fragment in the corresponding region (map units 77 through 89) of the mouse adenovirus type 1 genome has been determined. analysis of the sequence revealed that the genes for the fiber and for the precursor to the hexon-associated protein, pviii, that usually flank the e3 region, are well conserved. however, many of the open rea ... | 1989 | 2531236 |
| effects of vaccines on the canine immune system. | the effects of several commercially available polyvalent canine vaccines on the immune system of the dog were examined. the results demonstrated that the polyvalent vaccines used in this study significantly suppressed the absolute lymphocyte count and that most of the polyvalent vaccines significantly suppressed lymphocyte response to mitogen, but had no effect on natural effector cell activity, neutrophil chemiluminescence, nor antibody response to canine distemper virus. the individual vaccine ... | 1989 | 2540897 |
| characterization of adenovirus hexon epitopes by monoclonal antibodies. | three-thousand hybridoma clones were produced using crystallized hexon of human adenovirus type 1 for immunization. elisa revealed 69 homospecific antibody producing clones. the monoclonals were reactive with both crystallized and non-crystallized purified soluble hexon preparations, as well as with the hexons derived from purified virions. hexon preparations of 10 different human adenovirus species (members of 4 different subgenera) were used for the characterization of 33 monoclonal antibodies ... | 1984 | 6085607 |
| the structural proteins of chick embryo lethal orphan virus (fowl adenovirus type 1). | chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contains at least 14 structural proteins with polypeptide molecular weights ranging from 100k to about 6k. a nomenclature of the celo virion polypeptides is presented and the molar proportion of each polypeptide has been estimated. the celo virus pentons were specifically released from the virion by dialysis against borate-based calcium-magnesium saline. the penton base (polypeptide iii, mol. wt. 92k) and two fibres were separated, ... | 1984 | 6092524 |
| dna-binding proteins of chick embryo lethal orphan virus: lack of complementation between early proteins of avian and human adenoviruses. | chick cells infected by chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contained four prominent virus-specific, structurally related dna-binding proteins with mol. wt. of 74k, 64k, 56k, 52k, and two minor forms. the celo virus dna-binding proteins were phosphorylated, delayed-early nuclear proteins. celo virus early proteins were expressed in bhk cells, but did not complement human adenovirus type 5 mutants with lesions e1a, e2a or e2b. moreover, celo virus dna-binding proteins ... | 1984 | 6092525 |
| genome organization of mouse adenovirus type 1 early region 1: a novel transcription map. | mouse adenovirus type 1 (mav-1) genomic dna from 8.9 to 13.7 map units was sequenced and the early region 1 (e1) transcription map was determined by s1 nuclease, primer extension, and northern analyses, and cdna sequencing. the e1 transcription map of mav-1 had marked dissimilarities from the conserved transcription maps of primate adenovirus e1s. one major e1a and two e1b mrnas were identified in overlapping transcription units. the single e1a mrna was composed of three exons; the last exon was ... | 1989 | 2543128 |
| incomplete particles of canine adenovirus type 1: occurrence and structure. | replication of canine adenovirus (cav) type 1 in dog kidney (dk) cells has been found to generate particles containing double-stranded dna molecules of size 6-9 kbp with protein moieties at their 5' termini. the particles formed a discrete band in cscl gradients at a density intermediate to that of intact virions and empty capsids. electron microscopy demonstrated that they were morphologically similar to intact virions. restriction enzyme digests and hybridization experiments revealed the exist ... | 1989 | 2815582 |
| [immune response of calves following vaccination with paramyxovirus parainfluenzae-3 and adenovirus-1 under different colostral immunity stresses]. | studied was the immune response of calves, with colostrum antibodies and without colostrum antibodies, immunised with different dosages of live bivalent vaccine against p. parainfluenzae-3 and adenovirus-1. for the group of calves with colostrum immunity, after single hypodermic immunisation with doses from 10(4) till 10(5) cpe50/cm3 for adenovirus, was not proved immune response, as a result of blocking of the vaccinal antigen of colostrum antibodies. for calves which missed these antibodies, t ... | 1987 | 2833005 |
| detection of antibody titers to bovine viruses in rabbit sera. | sera obtained from 30 new zealand white rabbits from four different colonies were evaluated for the presence of immunoglobulins to 10 bovine viruses. serum neutralizing antibody titers to bovine adenovirus type 1 and to the enteroviruses types 3 and 4 were detected. | 1980 | 6253745 |
| hind iii restriction site map of the human adenovirus type 1 dna. | the physical map of human adenovirus type 1 dna was constructed with the hind iii restriction enzyme. direct comparison of the dna fragments with those of adenovirus type 2 revealed that the genome of adenovirus type 1 is 200 to 300 base pairs longer. the difference are located outside the region of the inverted terminal repetition within three distinct loci. fragment hind iii-f of type 1 dna which is presumed to carry all the genes responsible for in vitro transformation can be separated withou ... | 1981 | 6263226 |
| canine viruses and multiple sclerosis. | serum and cerebrospinal fluid from multiple sclerosis (ms) patients and control subjects were tested and compared for presence and titer of neutralizing antibody against the most common canine viruses. canine viruses included canine distemper virus (cdv), canine adenovirus 1 (cav-1), canine parainfluenza virus (cpiv), canine herpesvirus (chv), canine coronavirus (ccv), and canine parvovirus (cpv). neutralizing titers against measles virus (mv) and human adenovirus 8 (ha8) were also tested. signi ... | 1981 | 6267515 |
| characterization of canine adenovirus type 1 isolated from american black bears. | viruses recovered from tissues taken at necropsy from american black bears were examined by use of immunofluorescence with polyclonal and monoclonal antibodies, virus neutralization with monoclonal antibodies, and restriction endonuclease analyses of the viral genomes. with these techniques, viruses were determined to be canine adenovirus type 1. seronegative dogs that were inoculated with the virus had clinical signs typical of infectious canine hepatitis, suggesting that the virus, which was v ... | 1988 | 2840839 |
| characterization of the genome of a vaccine strain of canine adenovirus type 1. | restriction endonuclease cleavage maps have been constructed for the genome of a canine adenovirus type 1 (cav-1) vaccine strain (cll; connaught laboratories, ltd., willowdale, ontario). restriction enzyme analyses were also carried out on cav-1 (cll) genomes isolated from viral stocks over 8 serial passages in a dog kidney cell line (dk 6722). the right hand 20% of the genome became more heterogeneous in size with increasing passage in dk 6722 cells due to deletions up to 3-4 kb, whereas the le ... | 1988 | 2852417 |
| isolation and characterization of six new genome types of human adenovirus types 1 and 2. | several of the 41 types of human adenovirus have been divided into genome types based on aberrant restriction endonuclease digestion patterns of viral dna. in the process of screening a large number of clinical adenovirus isolates by restriction endonuclease digestion of viral dna, we have identified nine isolates from eight patients which were identified by neutralization as adenovirus type 1 or 2 but had aberrant cleavage patterns. cleavage sites for the enzymes smai, ecori, hindiii, kpni, and ... | 1985 | 2981899 |
| a persistent adenovirus type 1 infection in synovial tissue from an immunodeficient patient with chronic, rheumatoid-like polyarthritis. | | 1985 | 2985091 |
| the 'blue eye' phenomenon. | diffuse clouding of the cornea ('corneal oedema', 'blue eye') of sudden onset and usually transient duration and with accompanying anterior uveitis, may be attributable to natural infection with canine adenovirus type 1 (ca-1) or to vaccination with live modified virus. it is now recognised that kerato-uveitis is a manifestation of type iii hypersensitivity in which immune complex formation resulting from the release of virus, especially from infected corneal endothelial cells, brings about corn ... | 1983 | 6304988 |
| unambiguous typing of canine adenovirus isolates by deoxyribonucleic acid restriction-endonuclease analysis. | viral deoxyribonucleic acid extracted from a limited number of cells infected with canine adenovirus type 1 or type 2 was cleaved with several restriction endonucleases. agarose gel electrophoresis of the limit digests showed stable differences between the canine adenovirus type 1 and type 2 cleavage patterns. rapid and accurate typing of large numbers of clinical isolates may thus be done by deoxyribonucleic acid restriction-endonuclease analysis. | 1983 | 6321002 |
| preferential clustering of viral dna sequences at or near the site of chromosomal rearrangement in fowl adenovirus type 1 dna-transformed cell lines. | all six transformants obtained by inoculating fowl adenovirus type 1 (celo virus) dna or its fragments into a rat cell line of normal karyotype had more than 50 copy-equivalents of viral dna sequences. each of the transformants had almost all if not all of these viral dna sequences clustered on a marker chromosome(s). although the marker chromosome(s) differed from one cell line to another, viral dna sequences preferentially clustered in or near the achromatic (or light-stained) region of the g- ... | 1987 | 3023693 |
| comparative analysis of the canav-1 and canav-2 genomes. | the molecular weights of canine adenovirus type 1 (canav-1, strain utrecht) and type 2 (canav-2, strain toronto a26/61) dnas were determined by contour length measurements and restriction endonuclease analysis. in each case, an average molecular weight value of 20 (+/- 0.5) x 10(6) daltons was obtained with both methods. similar in size, the canav-1 and canav-2 dna molecules were, however, cleaved at very distinct positions by a variety of restriction endonucleases. the observed lack of dna sequ ... | 1986 | 3032842 |
| multiple reiteration of a 40-bp nucleotide sequence in the inverted terminal repeat of the genome of a canine adenovirus. | the dna of a vaccine strain of canine adenovirus type 1 [ichv vaccine; connaught laboratories, ltd.; cav-1(cll)] has been cloned in plasmid pat153 in the form of subgenomic bamhi digestion fragments. analysis of the nucleotide sequences of cloned terminal fragments has revealed an inverted terminal repeat (itr) with a minimum length of 198 nucleotides, including a tandem reiteration of the 40-bp nucleotide sequence from positions 14 to 53. the itrs had the 5'-catcatcaat ... sequence typical of a ... | 1987 | 3037784 |
| changes of adenovirus hexon associated with different passage history of ad h 1. | two descendants of the prototype strain ad71-washington d. c. were obtained by independent passaging for at least 18 years in kiev, and in budapest (ad h 1 kappa, and ad h 1b, respectively). by restriction endonuclease mapping, the dna was identical corresponding to the patterns of human adenovirus type 1. in spite of this, sds-polyacrylamide gel electrophoresis revealed that the purified hexon of ad h 1 kappa was of lower mr than the subunit of ad h 1b. in contrast to this, the native capsomer ... | 1986 | 3105225 |
| monoclonal antibodies to canine adenoviruses 1 and 2 that are type-specific by virus neutralization and immunofluorescence. | monoclonal antibodies were produced against the mirandola strain of canine adenovirus type 1 (cav-1) and the manhattan strain of canine adenovirus type 2 (cav-2). the monoclonal antibodies were used in vitro in virus neutralization (vn) assays and in indirect fluorescent antibody (ifa) tests to examine several strains of each virus type. out of 36 monoclonal antibodies produced against the mirandola strain, 18 were type-specific for cav-1 by ifa and 13 of those neutralized the virus in vitro. th ... | 1988 | 3281371 |
| infection rates of respiratory syncytial virus in pediatric patients attending phra mongkutklao hospital, bangkok. | respiratory syncytial virus (rsv) and other pathogens were isolated from nasopharyngeal secretions from 200 pediatric patients attending the out patient department of phra mongkutklao hospital with symptoms of upper respiratory tract infections. their sera were also taken for determination of class specific immunoglobulin antibody titers. the positive isolation rates were 36% for rsv, 5.5% for adenovirus 1.5% for herpes simplex virus (hsv), and 4% for staphylococcus aureus. one to 5.5% of these ... | 1984 | 6740381 |