| fiber sequence heterogeneity in subgroup f adenoviruses. | the fiber gene of adenovirus type 41 was sequenced and compared to the fiber gene sequence of adenovirus type 40 (a. h. kidd and m. j. erasmus, 1989, virology 172, 134-144), the other known member of subgroup f. the open reading frame, from map units 87 through 92 with transcription from the r-strand, comprised 1686 bases and was 45 bases longer than its counterpart on the ad40 genome. the 45-base difference appears to have resulted from a block deletion on the ad40 sequence. apart from this one ... | 1990 | 2219717 |
| human enteric adenovirus type 41 (tak) contains a second fiber protein gene. | | 1990 | 2336370 |
| dna hybridization for diagnosis of enteric adenovirus infection from directly spotted human fecal specimens. | by using a genomic probe, dna hybridization for adenovirus type 41 (ad41) showed equivalent sensitivity with a direct spot method from clinical specimens compared with a more laborious dna phenol extraction procedure. by using this direct spot preparation method, fecal specimens of 67 patients were examined under code for blind testing for the presence of adenovirus by dna hybridization by using two ad41 probes (genomic and cloned bglii-d) and an adenovirus type 2 genomic probe. identical result ... | 1987 | 2822761 |
| two new serotypes of enteric adenovirus causing infantile diarrhoea. | fastidious enteric adenovirus have recently been recognized as an important cause of acute gastroenteritis in young children. their inability to grow in vitro has hampered classification by conventional methods. with modern immunological and chemical techniques the enteric adenoviruses have been shown to be distinct from the 39 established human adenovirus serotypes. in a prospective study of the viral, bacterial and parasitic aetiology of acute gastroenteritis 410 children and 205 age-matched c ... | 1983 | 6307784 |
| identification of adenoviruses in faeces from patients with diarrhoea at the hospitals for sick children, london, 1989-1992. | faecal samples from 137 patients that had been shown to contain adenoviruses by electron microscopy were identified in a series of enzyme immunoassays (eia) using a single monoclonal antibody (mab) to adenovirus 40 and four different mabs to adenovirus 41. adenoviruses were partially characterised by restriction enzyme analysis (rea) of dna extracts using smai. samples were also run in a commercial eia (adenovirus ideia; dako, ltd.) which detects group antigen. the majority (84%) of adenoviruses ... | 1994 | 8083654 |
| relevance of commercial diagnostic tests to detection of enteric adenovirus infections in south africa. | the prevalence of enteric adenoviruses detected by an in-house enzyme-linked immunosorbent assay (the rivm-elisa) ranged from 13 to 38%, and subgroup f adenoviruses comprised 86%. all subgroup f adenoviruses reacted with both rivm anti-adenovirus type 40 (ad40) and anti-adenovirus type 41 (ad41) monoclonal antibodies but were not detected by adenoclone type 40/41 enzyme immunoassay (eia). the correlation between the biotrin eia and rivm-elisa results was low (26%). immunospecific tests suggest t ... | 2000 | 10747163 |
| [community gastroenteritis caused by adenovirus type 41]. | viral gastroenteritis is caused mainly by nv (norovirus). rotavirus, astrovirus and adenovirus are the major cause of gastroenteritis in humans although there are rare cases. from the end of june to the beginning of july 2002, we had an endemic of community gastroenteritis by adenovirus. in our investigation, the patients were separated into 3 groups. on comparison of the viruses from each groups we observed that they had the same characteristics. in conclusion, we found that the infection was c ... | 2003 | 14768347 |
| human enteric adenovirus type 41 binding to hep-2 cells is specific. | properties of human enteric adenovirus type 41 (ad41) binding to its receptor on the surface of hep-2 cells were investigated. the binding was found to be temperature-dependent, saturable, and specific. analysis of the binding data showed a single class of 4.3 x 10(4) receptor sites per cell, an equilibrium dissociation constant of 21.0 nm, and no cooperativity among receptor sites. trypsin-treated hep-2 cells subsequently grown in the presence of tunicamycin or 2-deoxyglucose recovered full ad4 ... | 1991 | 2053291 |
| the e1b transcription map of the enteric adenovirus type 41. | enteric adenovirus type 41 (ad41) is defective for growth in conventional established cell lines. ad41 is dependent on the ad5 early regions e1a/e1b since it cannot grow in hek cells but only in 293 hek cells transformed by ad5 e1 region. however, hep-2 cells have also been shown to support the growth of ad41 to some extent. the nucleotide sequence of the e1b region of the ad41 strain d389 has been determined. when compared to the corresponding region of the ad41 prototype strain (tak) the degre ... | 1992 | 1533079 |
| molecular epidemiology of subgenus f adenoviruses associated with pediatric gastroenteritis during eight years in hiroshima prefecture as a limited area. | we have studied the prevalence of the subgenus f adenoviruses and the molecular characteristics of adenovirus type 41 in hiroshima prefecture, japan, as a limited area during the period of 1997-2004. subgenus f adenoviruses were detected in 30 (3.4%) of 892 fecal specimens by enzyme immunoassay (eia), and 80.0% (24 of 30) of positive patients were <36 months old. one (3.3%) and 29 (96.7%) of the 30 eia-positive specimens were adenoviruses type 40 (ad40) and 41 (ad41), respectively. the genomes o ... | 2006 | 16847553 |
| enteric adenovirus type 41 isolates: cloning, physical maps and diversity in restriction enzyme cleavage pattern. | adenovirus (ad) type 40 and 41 dnas were directly extracted from stool specimens of children with gastroenteritis. two new strains of ad41, sanekata and ehime strain, were cloned and their restriction maps were constructed. the left terminal end of the cloned ad41 genome, ecori-e fragment of the sanekata strain and ecori-f fragment of the ehime strain, had transforming ability in rat 3y1 cells. only one of the 35 isolates of ad40 tested showed a different restriction profile, while three differe ... | 1992 | 1337571 |
| restriction analysis of the prototype strain of enteric adenovirus type 41 using exonuclease iii. | enteric adenoviruses 40 and 41 (ad40 and ad41) are a prominent cause of gastroenteritis in young children. diagnosis of these enteric types by conventional means is complicated by their fastidious growth characteristics. enteric adenovirus growth was enhanced by cocultivation. typing of enteric isolates currently entails analysis of the extracted viral dna with restriction enzymes. restriction endonuclease fragments of the ad41 strain tak genome were ordered by (i) double digestion, (ii) release ... | 1992 | 1322930 |
| characterizations of adenovirus type 41 isolates from children with acute gastroenteritis in japan, vietnam, and korea. | genetic and antigenic characterizations of 70 strains of adenovirus type 41 (ad41), isolated between 1998 and 2001 from children in japan, vietnam, and korea, were done by dna restriction enzyme (re) analysis, sequencing analysis, and monoclonal antibody (mab)-based enzyme-linked immunosorbent assay (elisa). eight genome types were observed in the present study, among which d25, d26, d27, and d28 were novel genome types. these eight genome types were divided into two genome-type clusters (gtcs) ... | 2004 | 15364986 |
| [studies on epidemic feature of enteric adenoviruses infection in feces of infants with diarrhea in shenzhen]. | to estimate the enteric adenoviruses infection in feces of infants with diarrhea in shenzhen. | 2001 | 11860852 |
| structural studies of human enteric adenovirus type 41. | enteric adenoviruses of serotypes 40 and 41 possess some specific structural features, one of which is the presence on the virion of two fibers of different lengths and primary sequences. these viruses are notoriously difficult to grow under laboratory conditions. in this paper the successful growth and purification of ad41 are presented in detail. structural ad41 proteins were analyzed by biochemical methods, mass spectrometry, and electron microscopy (em), in order to identify and localize the ... | 2002 | 11853401 |
| [preparation of antiserum to protein v of human adenovirus type 41]. | the fastidious property of human adenovirus type 41 (ad41) may be resulted from inadequate expression of protein v (pv), the minor core protein of adenovirus, in packaging cells. in this report, we prepared antiserum to pv of ad41 and for study the mechanism of ad41 fastidiousness. | 2009 | 19637578 |
| adenovirus type 41 lacks an rgd alpha(v)-integrin binding motif on the penton base and undergoes delayed uptake in a549 cells. | human adenovirus (ad) types 2, 3 and 12 are known to interact with cell surface integrins alpha(v)beta(3) and alpha(v)beta(5) through an rgd motif carried by the penton base. this interaction is thought to augment virus entry after initial contact between the fiber and specific receptor(s). ad40 and ad41 are the only members of the human subgroup f adenoviruses. the penton base protein sequence of one ad40 strain is known to carry the motif rgad rather than rgd, suggesting that not all human ade ... | 1999 | 10518709 |
| rapid shell vial culture technique for detection of enteroviruses and adenoviruses in fecal specimens: comparison with conventional virus isolation method. | detection of enteroviruses and adenoviruses mainly in fecal specimens by rapid culture with inoculation onto cell monolayers in flat-bottom tubes by centrifugation and immunofluorescence staining with genus-specific monoclonal antibodies was compared with that by the conventional virus isolation procedure. for both conventional culture and shell vial culture human lung fibroblast cells and tertiary monkey kidney cells were used. for enterovirus detection, 979 clinical specimens (916 stool specim ... | 1998 | 9738034 |
| fate of human enteric viruses during dairy manure-based composting. | murine norovirus 1 (mnv-1), aichi virus (aiv), and human adenovirus 41 (ad41) were seeded in dairy manure and composted for 60 days, and both the stability of virus genomes and infectious viruses were evaluated. for compost started in late fall, pile temperature reached approximately 54.5 degrees c on day 1 and remained between 55 and 60 degrees c for 3 days. for viral genomes, aiv had an approximate 1.4-log loss of viral genome after 1 day and more than a 3.1-log loss after 2 days; while for mn ... | 2010 | 20819370 |
| structural features unique to enteric adenoviruses. | enteric adenoviruses are important agents of pediatric gastroenteritis. characterization of monoclonal antibodies against human adenovirus 41 (h-41) identified an epitope of interest on protein vi, an internal virion protein. the epitope is common to enteric adenoviruses (subgenus a: h-12, h-18, h-31 and subgenus f: h-40, h-41) but is not shared by non-enteric serotypes (subgenera b, c, d or e). by expressing random oligonucleotide fragments of the protein vi gene as t7 gene 10 fusion proteins i ... | 1996 | 9015127 |
| genetic organization, size, and complete sequence of early region 3 genes of human adenovirus type 41. | the complete nucleotide and predicted amino acid sequences for open reading frames (orfs) of the human adenovirus type 41 (ad41) early region 3 (e3) gene have been determined. the sequence of the ad41 e3 gene (map units 74 to 83.9) consists of 3,373 nucleotides and has one tata box and two polyadenylation signals (aataaa). analysis of the nucleotide sequence reveals that the e3 gene can encode six orfs, designated rl1 to rl6. these are all expressed at the mrna level, as determined by reverse tr ... | 1996 | 8642703 |
| gastroenteritis due to adenovirus type 41 in an adult with chronic lymphocytic leukemia. | we describe a case of gastroenteritis due to adenovirus type 41 that lasted for 46 days (until the patient's death) in a 56-year-old woman with terminal b cell chronic lymphocytic leukemia. adenovirus particles were detected by electron microscopy in five stool specimens collected between 5 and 39 days after the onset of the diarrheal illness. the virus was identified as adenovirus type 41 by viral neutralization tests and other assays. we discuss the potential pathogenicity of subgenus f adenov ... | 1994 | 7986904 |
| human adenovirus type 41 contains two fibers. | dna sequencing of the subgroup f human adenovirus serotype 41 (tak, ad41) fiber gene revealed the presence of two adjacent open reading frames encoding information for proteins with molecular weights of 60.6 kda and 41.4 kda (pieniazek, et al; nucleic acids res. 18: p. 1901, 1990). in this paper, various approaches were used to characterize the two proteins and determine whether both fibers were expressed in infected cells as well as on viral particles. we initially used a reverse transcriptase- ... | 1994 | 7975882 |
| detection of emerging and re-emerging pathogens in surface waters close to an urban area. | current knowledge about the spread of pathogens in aquatic environments is scarce probably because bacteria, viruses, algae and their toxins tend to occur at low concentrations in water, making them very difficult to measure directly. the purpose of this study was the development and validation of tools to detect pathogens in freshwater systems close to an urban area. in order to evaluate anthropogenic impacts on water microbiological quality, a phylogenetic microarray was developed in the conte ... | 2015 | 26006125 |
| [an outbreak of enteric adenovirus type 41 endemic in fjieda, japan]. | to determine the incidence of enteric adenovirus, 447 stool specimens were collected between january 1991 to october 1992 from children with gastroenteritis at a clinic in fujieda, japan. each specimen was tested for adenovirus group antigen and for specific enteric adenovirus types 40 and 41 (ad40/41) by using monoclonal antibody enzyme immunoassays. adenovirus was found in 10.7% (48 of 447) cases and 77.1% (37 of 48) of them were ad40/41. there were two peaks of adenovirus infection may and no ... | 1994 | 7876667 |
| direct detection and differentiation of fastidious and nonfastidious adenoviruses in stools by using a specific nonradioactive probe. | restriction fragments of fastidious human adenovirus type 41 (ad41) were cloned in vector plasmid pbr322. a rapid and sensitive nonradioactive molecular-hybridization technique (m. renz and c. kurz, nucleic acids res. 12:3435-3444, 1984) showed that one clone specifically detected fastidious ad40 and ad41 (subgenus f) without cross-hybridization with nonfastidious adenoviruses. this clone was mapped in a region of the ad41 genome corresponding to early transcription unit e1b of ad2. a number of ... | 1986 | 3771764 |
| dna sequence of the adenovirus type 41 hexon gene and predicted structure of the protein. | the gene for the major capsid protein (hexon) of human adenovirus type 41 (ad41) has been isolated and the complete dna sequence determined. comparison of the predicted amino acid sequence with hexons from human ad2 and ad5 and bovine adenovirus type 3 reveals regions of high homology at the n and c termini separated by a central region of low homology. fitting of the ad41 hexon sequence to the known three-dimensional structure of the ad2 hexon demonstrates that both hexons have a common archite ... | 1988 | 3411299 |
| monoclonal antibody enzyme-linked immunosorbent assay for specific identification and typing of subgroup f adenoviruses. | monoclonal antibody specific for subgroup f enteric adenoviruses (eads) was prepared by fusing p3-ns1/ag4-1 mouse myeloma cells with lymphocytes from balb/c mice immunized with g1105, an adenovirus type 41 (ad41) strain. monoclone 3f11/2h9, which specifically recognized ad41, was successfully used as detector antibody in an enzyme-linked immunosorbent assay (elisa). additionally, previously prepared monoclones 5d8/2c2 and 2h6/1e11, recognizing ad40 plus ad41 and ad40 alone, respectively, were us ... | 1988 | 3343324 |
| physical organization of the enteric adenovirus type 41 early region 1a. | enteric adenovirus types 40 and 41 (ad40 and ad41), representing subgenus f, differ from all other human adenoviruses by being so fastidious that productive replication does not occur in conventional established cell lines. they are dependent of the ad5 early regions e1a and e1b since they can not grow in hek cells, only in 293 hek cells transformed by ad5 e1. the overall genetic organization of ad41 e1a is similar to the e1a region of other characterized human adenoviruses but it is slightly sh ... | 1988 | 2834871 |
| nucleotide sequence of human enteric adenovirus type 41 hexon-associated protein viii precursor (pviii) including the early region e3 promoter. | | 1989 | 2762136 |
| characterization of fastidious adenovirus types 40 and 41 by dna restriction enzyme analysis and by neutralizing monoclonal antibodies. | the dna of 48 strains of adenovirus type 40 (ad40) and of 128 strains of adenovirus type 41 (ad41), isolated between 1971 and 1986 from various countries, was characterized by restriction enzyme analysis using nine and ten restriction endonucleases respectively. five new dna variants of ad40 and 18 new dna variants of ad41 were detected. most of the restriction sites which differed among the various dna variants appeared to be distributed at random over the entire length of the viral genomes of ... | 1989 | 2705331 |
| sequence of human enteric adenovirus type 41 tak fiber protein gene. | | 1989 | 2587268 |
| development and evaluation of monoclonal antibody-based immune electron microscopy for diagnosis of adenovirus types 40 and 41. | immune electron microscopy based on monoclonal antibodies was developed and evaluated for diagnosis of adenovirus type 40 and adenovirus type 41 directly from clinical specimens. one adenovirus type 40 monoclonal (5-8) and one adenovirus type 41 monoclonal (5-15) were found to react to high titre with homotypic but not heterotypic antigen. these monoclonals were tested on a coded batch of 20 stools which contained adenovirus type 40 or adenovirus type 41. the results showed that 5/6 adenovirus t ... | 1989 | 2584348 |
| evaluation of a commercial monoclonal antibody-based enzyme immunoassay for detection of adenovirus types 40 and 41 in stool specimens. | a commercial monoclonal antibody-based enzyme immunoassay (adenoscreen; mercia diagnostics ltd., guildford, united kingdom) for the detection of adenovirus types 40 and 41 in stool specimens was evaluated. two assay modes were tested. in the first, 177 stool samples were screened for the presence of adenovirus type 40 or 41 (assay mode 1). virus was detected in 79 of 82 specimens positive for adenovirus type 40 or 41 by a polyclonal antibody-based immune electron microscope test, giving a sensit ... | 1989 | 2546968 |
| nucleotide sequence of the region coding for 100k and 33k proteins of human enteric adenovirus type 41 (tak). | | 1990 | 2349115 |
| adenovirus 41 growth in semi-permissive cells shows multiple-hit kinetics. | adenovirus type 41 infection of semi-permissive chang conjunctival cells, monitored by fluorescent focus assay, followed multiple-hit kinetics. in non-permissive human cells, type 41 showed infectivity with two-hit kinetics in the presence of type 2. type 41 infectivity was seen to be directly proportional to input concentration (one-hit) only in 293 cells, a continuous human line expressing ad 5 e1 products. | 1990 | 2317153 |
| [improved replication of enteric adenovirus type 41 in hep2 cell line expressing e1b55k]. | adenovirus type 40 and 41 (ad40, ad41), which belong to human adenovirus subgroup f, are called fastidious adenoviruses due to their property of poor growth in cultured cell lines in vitro the effect of expression of exogenous e1b55k in hep2 on ad41 replication in this cell line was investigated. e1b55k gene was amplified by pcr with dna extracted from ad41-positive feces supernatant as template. eukaryotic expression plasmid (pcdna3) carrying e1b55k was constructed, purified, and transferred in ... | 2007 | 17894226 |
| novel recombinant adenovirus type 41 vector and its biological properties. | human adenovirus serotype 41 (ad41) is a natural pathogen of the digestive tract and can cause gastroenteritis. there has been interest in reconstructing ad41 as a gene delivery vector targeting the gastrointestinal tract, which is hampered by its fastidiousness. | 2009 | 19097028 |
| quantitative detection of human adenoviruses in wastewater and combined sewer overflows influencing a michigan river. | enteric viruses are important pathogens found in contaminated surface waters and have previously been detected in waters of the great lakes. human adenoviruses were monitored because of their high prevalence and persistence in aquatic environments. in this study, we quantified adenoviruses in wastewater, surface water, and combined sewer overflows (csos) by real-time pcr. between august 2005 and august 2006, adenovirus concentrations in raw sewage, primary-treated effluent, secondary-treated eff ... | 2010 | 19948848 |
| environmental surveillance and molecular characterization of human enteric viruses in tropical urban wastewaters. | to study the prevalence and genotypes of waterborne pathogenic viruses in urban wastewaters in the tropical region. | 2010 | 20233263 |
| outbreak of acute gastroenteritis caused by adenovirus type 41 in a kindergarten. | summaryin response to an alert due to epidemic gastroenteritis in children in a kindergarten, an outbreak investigation was carried out in a portuguese municipality. the objectives were to establish an aetiological diagnosis, assess vaccine efficacy if possible, and to take corrective measures if necessary. the warden at the kindergarten was interviewed, and we visited the premises. the overall attack rate was 11·4% and most cases were mild. stool samples from three symptomatic children were col ... | 2010 | 21156097 |
| block in entry of enteric adenovirus type 41 in hek293 cells. | human species f adenoviruses, hadv-40 and hadv-41, display characteristic gut tropism in vivo as well as poor infectivity in cell culture. to address the hypothesis that poor infectivity of hadv-40/41 reflects a partial block prior to genome delivery, the internalization and trafficking of hadv-41, hadv-5 (species c) and hadv-35 (species b) were compared in 293 (human embryonic kidney) cells, which complement e1b function in hadv-40/41, and in a549 (lung epithelial) cells. unlike fluorescently l ... | 2011 | 21215283 |
| an improved hadv-41 e1b55k-expressing 293 cell line for packaging fastidious adenovirus. | human adenovirus type 41 (hadv-41) is difficult to cultivate under laboratory conditions. tripartite leader sequence (tpl) of hadv-41 was cloned, inserted into eukaryotic expression plasmid, and used to establish a hadv-41 e1b55k-transduced cell line (293te7). hadv-41 e1b55k was expressed more abundantly in 293te7 than in 293e12, an hadv-41 e1b55k-expressing cell line developed previously. after being infected with e1-deleted hadv-41 vector (hadv-41-gfp), 293te7 synthesized more viral genomic dn ... | 2011 | 21601594 |
| generation and biological properties of a recombinant dodecahedron containing the short fiber protein of the human adenovirus 41. | objective: in order to gain further insight into the function of the enteric adenovirus short fiber (sf), we have constructed a recombinant dodecahedron containing the sf protein of hadv-41 and the hadv-3 penton base. methods: recombinant baculoviruses expressing the hadv-41 sf protein and hadv-3 penton base were cloned and amplified in sf9 insect cells. recombinant dodecahedra were expressed by coinfection of high five™ cells with both baculoviruses, 72 h post-infection. cell lysate was centrif ... | 2011 | 22057164 |
| inefficient export of viral late mrna contributes to fastidiousness of human adenovirus type 41 (hadv-41) in 293 cells. | the human adenovirus (hadv) early protein e1b55k interacts with e4orf6 to form an e3 ubiquitin ligase complex, which plays key roles in virus replication. to illustrate the reason for the fastidiousness of hadv-41 in 293 cells, interaction between heterotypic e1b55k and e4orf6 proteins was investigated. hadv-5 e1b55k could interact with hadv-41 e4orf6, and vice versa. to form e1b55k/e4orf6 e3 ubiquitin ligase, hadv-41 e4orf6 recruited cul2 while hadv-5 e4orf6 interacted with cul5. the ligase com ... | 2014 | 25240325 |
| on-chip isothermal nucleic acid amplification on flow-based chemiluminescence microarray analysis platform for the detection of viruses and bacteria. | this work presents an on-chip isothermal nucleic acid amplification test (inaat) for the multiplex amplification and detection of viral and bacterial dna by a flow-based chemiluminescence microarray. in a principle study, on-chip recombinase polymerase amplification (rpa) on defined spots of a dna microarray was used to spatially separate the amplification reaction of dna from two viruses (human adenovirus 41, phi x 174) and the bacterium enterococcus faecalis, which are relevant for water hygie ... | 2016 | 26624222 |
| uv inactivation of adenovirus type 41 measured by cell culture mrna rt-pcr. | adenoviruses are among the most resistant waterborne pathogens to uv disinfection, yet of the 51 serologically distinct human adenoviruses, only a few have been evaluated for their sensitivities to uv irradiation. human enteric adenoviruses (ad40 and ad41) are difficult to cultivate and reliably assay for infectivity, requiring weeks to obtain cytopathogenic effects (cpe). inoculated cell cultures often deteriorate before the appearance of distinctive cpe making it difficult to obtain reliable a ... | 2005 | 16046229 |
| outcome of hematopoietic stem cell transplantation in severe combined immune deficiency with central nervous system viral infection. | patients with severe combined immunodeficiency and preexisting viral pneumonitis formally had a poor outcome from hematopoietic stem cell transplantation. with inhaled steroid and antitumor necrosis factor alpha antibody treatment, results improved. the poor outcome of patients with viral central nervous system infection prompted this retrospective single center review. | 2007 | 17259874 |
| new adenovirus species found in a patient presenting with gastroenteritis. | an unidentified agent was cultured in primary monkey cells at the los angeles county public health department from each of five stool specimens submitted from an outbreak of gastroenteritis. electron microscopy and an adenovirus-specific monoclonal antibody confirmed this agent to be an adenovirus. since viral titers were too low, complete serotyping was not possible. using the dnase-sequence-independent viral nucleic acid amplification method, we identified several nucleotide sequences with a h ... | 2007 | 17360747 |
| prevalence and characterization of enteric adenoviruses in the south of ireland. | enteric adenoviruses have been shown to be a substantial cause of pediatric gastroenteritis in various parts of the world, and are considered to be the second most common cause of viral gastroenteritis, next to rotavirus in young children. genetic characterization of 95 adenovirus isolates obtained from patients with acute gastroenteritis between 2002 and 2007 from the southern regions of ireland, were characterized by pcr analysis, restriction endonuclease (re) analysis and sequencing analysis. ... | 2007 | 17705179 |
| development of a new cell culture-based method and optimized protocol for the detection of enteric viruses. | the development of rapid and effective methods to detect water- and food-borne enteric viruses is important for the prevention and control of mass infection. this study represents an attempt to develop a reliable cell culture-based detection system and optimize an effective and rapid protocol for the assaying of environmental samples for the presence of infectious enteric viruses. six enteric viruses were used in this study: poliovirus, coxsackie virus a9, coxsackie virus b5, human rotavirus g1, ... | 2013 | 23578702 |
| [rescue and amplification of recombinant human adenovirus type 41 in 293 cells]. | human adenovirus type 41 (hadv-41) is considered to be a "fastidious adenovirus". e1-deleted hadv-41 cannot be rescued or amplified in 293 cells. to propagate recombinant hadv-41 in 293 cells, the backbone plasmid padbone41 was reconstructed. that is, the e3 coding sequence of hadv-41 was deleted and replaced with the hadv-5 e4orf6 gene; and the e1a enhancer of hadv-5 was inserted upstream of the e4 promoter of hadv-41. novel adenoviral plasmid pad41e4ee-gfp was generated by homologous recombina ... | 2015 | 26738289 |
| human adenovirus type 41 possesses different amount of short and long fibers in the virion. | to determine the ratio of short fiber (sfiber) to long fiber (lfiber) in human adenovirus type 41 (hadv-41) virions, sfiber and lfiber were expressed in e. coli, quantified, and used as loading standards in western blot. densitometric analyses of the standard and target bands indicated that the ratio of sfiber to lfiber in virions was 5.7±0.7. sfiber-deleted hadv-41, hadv-41-dsf-gfp, was constructed, and western blot analysis showed that the amount of lfiber in hadv-41-dsf-gfp was about 7.3±1.9 ... | 2012 | 22727834 |
| construction of an infectious clone of human adenovirus type 41. | human adenovirus type 41 (hadv-41) is well known for its fastidiousness in cell culture. to construct an infectious clone of hadv-41, a dna fragment containing the left and right ends of hadv-41 as well as a kanamycin resistance gene and a pbr322 replication origin was excised from the previously constructed plasmid pad41-gfp. using homologous recombination, the plasmid pkad41 was generated by co-transformation of the e. coli bj5183 strain with this fragment and hadv-41 genomic dna. virus was re ... | 2012 | 22527861 |
| inactivation of internalized and surface contaminated enteric viruses in green onions. | with increasing outbreaks of gastroenteritis associated with produce, it is important to assess interventions to reduce the risk of illness. uv, ozone and high pressure are non-thermal processing technologies that have potential to inactivate human pathogens on produce and allow the retention of fresh-like organoleptic properties. the objective of this study was to determine if uv, ozone, and high pressure are effective technologies compared to traditional chlorine spray on green onions to reduc ... | 2013 | 23973828 |
| assessing uv inactivation of adenovirus 41 using integrated cell culture real-time qpcr/rt-qpcr. | enteric adenoviruses are among most uv-resistant viruses in water. cytopathic effects (cpe)-based cell culture tcid50 assay as a conventional virus assessment approach has major drawbacks for enteric adenovirus since it is selective on cell lines and takes longer time to show cpe. integrated cell culture real-time quantitative pcr (icc-qpcr) and reverse transcriptase (rt)-qpcr were applied in this study, in comparison with tcid50, to assess uv inactivation of adenovirus type 41 (ad41) in water. ... | 2017 | 28377001 |
| prevalence of serum neutralizing antibodies to adenovirus type 5 (ad5) and 41 (ad41) in children is associated with age and sanitary conditions. | neutralizing antibody (nab) can dampen the immunogenicity of adenovirus (ad) vector-based vaccine. vector systems based on human adenovirus type 41 (ad41) have been constructed and used to develop recombinant vaccines. here, we attempted to study the seroprevalence of nabs to ad5 and ad41 among children and adults in qinghai province, china. the positive rates (titer⩾40) of ad5 and ad41 nab in adults from xining city were 75.7% and 94.7%, respectively. the moderate/high-positive rates (titer⩾160 ... | 2016 | 27682509 |
| viral gastroenteritis in rotavirus negative hospitalized children <5 years of age from the independent states of the former soviet union. | rotavirus causes nearly 40% of all hospitalizations for age among children <5 years of age in the nis of the former soviet union. the etiologic role of other established gastroenteritis viruses in this age group is unknown. | 2014 | 25460823 |
| [morphogenetic study of human adenovirus type 41 in 293te cells]. | to investigate the morphogenetic process of human adenovirus type 41 (hadv-41), 293te cells were infected with purified wild-type hadv-41, and ultrathin sections of infected cells were prepared and observed under a transmission electron microscope. results showed that hadv-41 entered host cells mainly through three ways: non-clathrin-coated pit, clathrin-coated pit, and direct penetration of plasma membrane. in addition, cell microvilli might help hadv-41 enter cells. after entering into cells, ... | 2014 | 24923169 |
| [characterization of fibrillous inclusion body (fib) of human adenovirus type 41 (ad41) using immunoelectron microscopy]. | to investigate the components of fibrillous inclusion body (fib), which was formed in packaging cells during the replication of human adenovirus type 41 (ad41), ad41 long fiber knob (lfk) and short fiber knob (sfk) proteins were expressed in prokaryote respectively and then used to immunize bali mice for preparation of anti-lfk serum and anti-sfk sera. the activity and specificity of anti-lfk and an ti-sfk sera were confirmed with western blot, indirect immunofluorescence assay (ifa) and immunon ... | 2013 | 24520764 |
| enhanced growth of recombinant human adenovirus type 41 (hadv-41) carrying adp gene. | human adenovirus type 41 (hadv-41) has the potential to be constructed as a gene transfer vector for oral vaccine or gene therapy targeting gastrointestinal tract. block in release of progeny virus from host cell severely affects the yield during virus amplification. in this study, hadv-5 adenovirus death protein (adp) gene was used to replace the open reading frames (orfs) of the hadv-41 e3 region to construct a backbone plasmid padbone41adp. recombinant adenoviral plasmids harboring adp and gf ... | 2013 | 23769974 |
| etiology of viral gastroenteritis in children <5 years of age in the united states, 2008-2009. | although rotavirus and norovirus cause nearly 40% of severe endemic acute gastroenteritis (age) in children <5 years of age in the united states, there are limited data on the etiologic role of other enteric viruses in this age group. | 2013 | 23757337 |
| an improved infectivity assay combining cell culture with real-time pcr for rapid quantification of human adenoviruses 41 and semi-quantification of human adenovirus in sewage. | a protocol for the rapid detection and semi-quantification of human enteric adenovirus based on the quantification of viral mrna during cell culture infectivity assay was developed. infectivity assays for adenovirus incorporated cell culture and reverse transcription real-time pcr, where viral mrna detection was used to monitor the progress of adenovirus infection (cc/mrna qpcr). the cell line used was g293. this specific infectivity assay was calibrated against different initial concentrations ... | 2013 | 23579085 |
| [investigation of adenovirus infection in hospitalized children with diarrhea during 2010 in beijing, china]. | the study was designed to evaluate adenovirus infection in hospitalized children with diarrhea. | 2012 | 22931944 |
| clinical evaluation of a bioluminescent enzyme immunoassay for detecting norovirus in fecal specimens from patients with acute gastroenteritis. | noroviruses (novs), which belong to the family caliciviridae, are major causative agents of acute gastroenteritis worldwide. thus, rapid and highly sensitive assays for detecting novs are required. recently, a bioluminescent enzyme immunoassay (bleia) for detecting novs in fecal specimens was developed. this new assay was evaluated using fecal specimens obtained from acute gastroenteritis patients. of the 107 specimens that were found to be nov-positive by rt-pcr or rt-lamp, 104 specimens produc ... | 2014 | 24114991 |