| adenovirus type 7; 1971-74. | the spread of adenovirus type 7 in england and wales between 1971 and 1974 and the clinical features of infections with this virus were investigated in a retrospective study of virological reports and patients' clinical records. an epidemic in 1972-74 apparently stared in the north-east and spread to the south-west. between march 1973 and the end of october 1974 the virus was recovered in 59 of 74 specimens from 42 patients. the mean age of the patients was 9 years, which suggests that a large p ... | 1976 | 62264 |
| fatal adenovirus infection with misleading positive serology for infectious mononucleosis. | symptoms and serological tests in a 19-year-old man with pneumonia indicated that he had infectious mononucleosis. adenovirus type 7 was demonstrated in the lung by electron microscopy and was cultured from the liver, lung, lymph-nodes, and kidney. | 1979 | 84950 |
| detection of herpes-simplex viral genome in brain tissue. | herpes-simplex virus type (hsv-1) nucleic-acid sequences were detected by in-situ hybridisation in thin sections of brains from mice which had been inoculated 24 weeks previously with hsv-1. these mice were not ill, and infectious virus could not be recovered from them. hsv-1 sequences were also present in brain smears from 3 of 4 elderly patients who had died with chronic psychiatric illness and neuropathological changes but not in smears from 2 patients who had had acute psychotic episodes and ... | 1979 | 90272 |
| late transcription and simultaneous replication of simian adenovirus 7 dna as revealed by spreading lytically infected cell cultures. | miller's technique of spreading dna was applied to monkey cells productively infected with simian adenovirus 7. this permitted the visualization of cellular dna transcription, both nucleolar and non-nucleolar, and of late transcription and replication of virus. virus double-stranded dna, thin fibres with very few nucleosome-like particles, were observed carrying either transcription or replication complexes. in addition, both rnp transcripts and replication forks were found on some virus duplex ... | 1979 | 107271 |
| [hemagglutination by simian adenovirus 7]. | simian adenovirus 7, either complete virus or its capsid subunits, agglutinates rat (sprague-dowley) red blood cells in the presence of heterotypical antiserum. haemagglutination takes place at 4 degrees c and room temperature. the antigen could not be eluted and its haemagglutinin properties are heat-stable. the reaction is specific. it is inhibited by homologous antiserum only. this property and its characteristics permit a camparison of this strongly oncogenic adenovirus to the human adenovir ... | 1979 | 108027 |
| biological activity of the intact and cleaved dna of the simian adenovirus 7. | only the deproteinized dna preparations of the simian adenovirus of the type 7 (sa 7) exhibited transforming and tumorigenic activity. the complex of the sa7 dna with terminal protein (tp) did not exhibit either transforming or tumorigenic activity in cell cultures. in contrast to the transforming potential the infectious titers of the dna - tp complex for the monkey kidney cells were 30-50 times higher than those of pure dna. cleavage of the sa7 dna by specific endonucleases enhanced the tumori ... | 1979 | 114980 |
| [modification of human adenovirus type 7 after culture in guinea pig brain cells in vitro]. | multiplication of human adenovirus 7 (ad7) is restricted in subcultures of adult guinea pig brain cells. it was however possible to recover enough infectious virus to purify it, and to study its properties. several properties are similar to that of ad 7 produced in hela cells but differences in sensitivity to cesium chloride and in its polypeptide composition were found. | 1979 | 121268 |
| stimulation of adenovirus replication in simian cells in the absence of a helper virus by pretreatment of the cells with iododeoxyuridine. | pretreatment of african green monkey kidney cells with 50 mu g of 5'-iododeoxyruidine (iudr) per ml can modify their susceptibility to the replication of human adenovirus type 7 in the absence of simian virus 40 (sv40) although this enhancement of adenovirus replication is not as efficient as that of the helper sv40 virus. since the number of infectious centers remains unchanged after iudr pretreatment whereas the burst size of virus from each infected cell increases, the iudr appears to allow e ... | 1975 | 163353 |
| adenovirus-transformed cells restrict herpes simplex virus replication. | a cell line that normally supports the replication of herpes simplex virus types 1 and 2 became resistant to these viruses after transformation by simian adenovirus 7. kinetic studies of the mechanism of resistance demonstrated that both herpesviruses were able to attach to the transformed cells and express some early genomic functions, as demonstrated by the presence of low levels of viral thymidine kinase. however, isopycnic centrifugation studies of the abortive system failed to detect viral ... | 1975 | 163798 |
| spleen cell-mediated cytotoxicity of hamster cells transformed by herpes simplex virus: evidence for virus-specific membrane antigen. | the nature of the host's immune response to isografts of hamster embryo fibroblasts (hef) transformed by herpes simplex virus type 1 (hsv-1) was investigated by the microcytotoxicity assay. it was found that spleen cells from tumor-bearing hamsters killed homologous tumor cells but not hef transformed by cytomegalovirus or para-(defective sv40)-adenovirus 7 (para-7). cytotoxicity was lost as the tumor increased in size. spleen cells from animals bearing isografts of hsv type 2 (hsv-2) transforme ... | 1975 | 163859 |
| letter: petechiae: due to adenovirus type 7 infection or to acetylsalicylic acid therapy? | | 1975 | 165451 |
| analysis of sera from sv40-immunized and tumor-bearing hosts for blocking activity and antibody-dependent cellular cytotoxicity. | the role of serum factors in tumor immunity to cells transformed by para-(defective sv40)-adenovirus 7 was investigated. it was found that sera from sv40-sensitized hosts did not block the specific cytotoxicity of sv40-sensitized spleen cells for para-7 cells. however, such sera could collaborate with nonsensitized spleen cells to produce specific killing. this activity could be absorbed out by para-7 cells but not by cells transformed by cytomegalovirus. the activity of sera from hamsters beari ... | 1975 | 168264 |
| inappropriate secretion of antidiuretic hormone associated with adenovirus pneumonia. | metabolic abnormalities compatible with inappropriate secretion of adh developed during the course of severe viral pneumonia in a 17-year-old navy recruit. with a regimen of strict fluid restriction, normalization of these abnormalities occurred. marked leukopenia and hypoxia were also present, but gradually improved with resolution of the pneumonia. inappropriate adh secretion has been associated most often with bacterial pneumonia and this patient represents one of the few with viral pneumonia ... | 1975 | 170045 |
| differential response to cytochalasin b among cells transformed by dna and rna tumor viruses. | mouse, hamster, rat, human, and chick cells were transformed by rna and dna tumor viruses: simian virus 40, adenovirus type 7, kirsten mouse sarcoma virus (ki-musv), moloney mouse sarcoma virus, and rous sarcoma virus. all cultures of transformed cells grew to high concentration densities. normal and transformed cells were treated with cytochalasin b (cb) at concentrations preventing cytoplasmic cleavage. cells altered by dna tumor viruses responded to cb with numerous nuclear divisions resultin ... | 1975 | 171432 |
| rhinovirus and influenza type a infections as precipitants of asthma. | specimens from 49 persons, 3 to 60 years of age, who were prone to attacks of apparently infectious asthma were cultured for viruses and bacteria during episodes of symptomatic respiratory infection from september 1972 to june 1973. seventy-one of 128 episodes (55 per cent) of symptomatic respiratory infection occurred with asthma. shedding of respiratory pathogens was associated with 43 of the 128 episodes, and 19 of the 43 etiologically defined episodes occurred with wheezing. seven of 15 rhin ... | 1976 | 174464 |
| suppression of the temperature-sensitive character of adenovirus 12 early mutants in monkey cells transformed by an adenovirus 7-simian virus 40 hybrid. | | 1976 | 176780 |
| coxsackievirus and adenovirus infection. association with acute febrile and juvenile rheumatoid arthritis. | the prearthritic manifestations of juvenile rheumatoid arthritis in a 16-year-old boy were associated with a rise in coxsackievirus b3 and a9 neutralizing antibody titers from 1:16 to larger than or equal to 1:512, and 1:64 to 1:512, respectively. recurrent polyarthritis followed and has persisted for three years. adenovirus 7 was isolated from the pericardial fluid of a 9-year-old girl in whom juvenile rheumatoid arthritis then developed. of 11 patients with acute, nonspecific, febrile arthriti ... | 1976 | 177792 |
| characterization of simian cells tranformed by temperature-sensitive mutants of simian virus 40. | seven lines derived from primary african green monkey kidney cells, which had survived lytic infection by wild-type simian virus 40 (sv40) or temperature-sensitive mutants belonging to the a and b complementation groups, were established. these cultures synthesize sv40 tumor (t) antigen constitutively and have been passaged more than 60 times in vitro. the cells released small amounts of virus even at high passage levels but eventually became negative for the spontaneous release of virus. virus ... | 1976 | 178917 |
| isolation and properties of reovirus from cattle in an outbreak of acute respiratory disease. | a cytopathogenic virus was isolated in the primary culture of bovine kidney cells from a nasal swab of affected calves in an outbreak of acute respiratory disease in japan in 1971. it agglutinated human type o erythrocytes and produced cytoplasmic inclusion bodies. viral replication was inhibited by 5-iodo-2'-deoxyuridine, indicating that the viral nucleic acid was rna. the virus was resistant to ether, chloroform, sodium deoxycholate, and acid, and passed readily through sartorius' membrane fil ... | 1976 | 181678 |
| separation and properties of enterovirus and reovirus recovered from a fecal sample of calf with diarrhea. | in april, 1971, a disease with pyrexia and diarrhea as main symptoms broke out collectively among calves. fecal samples were collected from calves involved and inoculated into bovine kidney (bk) cell cultures. as a result, the diarrheal feces of one calf were suspected to contain two agents simultaneously. one agent (c-121 e strain) was isolated from the primary infected bk cell culture fluid by terminal dilution passages. it had been predominant in replication and shown a cytopathic effect whic ... | 1976 | 181679 |
| isolation of a subgroup two adenovirus from calf with weak calf syndrome. | a viral agent, designated id-1, was isolated from the buffy coat of a calf suffering from weak calf syndrome. the virus replicated on bovine salivary gland cells and caused cytopathic effect within four days after infection-cytopathic effect was characterized by rounding and clumping of cells. stained preparations of infected monolayers revealed multiple intranuclear inclusions. the agent was found to be resistant to chloroform, ether, trypsin, sodium desoxycholate, oxytetracycline and a ph rang ... | 1976 | 187293 |
| lacalization of the sv40 t antigen in hamster cells transformed by para(3ct)-adenovirus 7. | the localization of the sv40 t antigen in hamster cells transformed by the para(3ct)-adenovirus 7 variant and, for comparison, by sv40 was examined. the h50 cell strain (transformed by sv40) and the p7-/bl/sv/od strain (transformed by para(3ct)-adenovirus 7) showed a preferential localization of the sv40 t antigen in the nucleus both by immunofluorescence and by complement fixation. on the other hand, two different para(3ct)-adenovirus 7-transformed cell lines (p7-/ar/d/15a and p7/ar/m/14a) show ... | 1975 | 187551 |
| adenovirus type 7 pneumonia associated with a large pleural effusion. | | 1977 | 190706 |
| character of the tumors developing in young hamsters by the mixed cell transplantation of para-adeno-virus 12 and sv40 tumors-the similarity to the tumors induced by para-adenovirus 7. | two mixtures of either allogeneic para-adenovirus 12 tumor cells or adenovirus (adeno) 12 tumor cells and sv40 tumor cells in an equal number of 1 multiplied by 10(7) cells were transplanted subcutaneously at two sites of 10 young hamsters. the former two tumor cells were transplantable in nearly half of the transplants and the latter tumor cells in all of the transplants. by the mixture of para-adeno 12 and sv40 tumor cells, 10 tumors were grown, 7 of which were mixed with adeno and sv40 types ... | 1977 | 200058 |
| [inappropriate secretion of adh during acute respiratory infections due to adenovirus in children (author's transl)]. | four children aged between 7 and 19 months with severe bronchopneumonia due to adenovirus type 7, proved by virology and/or serology developed severe hyponatraemia. one of them is reported in detail: it was possible to estimate plasma adh levels and thereby prove the existence of reversible hypersecretion of the hormone. whilst the syndrome of hyponatraemia with inappropriate secretion of adh has not yet been reported in association with severe pneumonia in the child, it is known in adults. the ... | 1977 | 201922 |
| a murine oncornavirus marker in adenovirus 7-transformed mouse cells. | the presence of oncornavirus particles in balb/c mouse cells transformed by adenovirus 7 was investigated. the particles detected had a buoyant density of 1.16 g/cm3; both p30 and adenovirus t-specific immunofluorescence was demonstrated in the cell cytoplasm. no c-particles were shown by the electron microscope. | 1977 | 203069 |
| [loss of steroidogenesis in a mouse adrenal cell line after simian adenovirus 7 (sa7) transformation]. | after transformation by the simian adenovirus 7 (sa7), the y-1 mouse adrenal tumor cells are no longer able to produce steroïds either spontaneously, or after specific stimulation. nevertheless, these stimulations are able to increase the level of camp both in transformed and original cell line. the differentiation is associated with an intracellular modification of steroïdogenesis which seems to be localized in the metabolic chain of steroïd synthesis after the increase of the camp level. | 1978 | 208792 |
| fatal adenovirus type 7 pneumonia in an adult. | a case of fatal pneumonia caused by adenovirus type 7 in an adult is reported. this virus was isolated from the lung tissue, and typical inclusion bodies were found in histological sections. death due to adenovirus pneumonia in adults appears to be extremely rare. | 1978 | 215888 |
| experimental intraamnionic exposure of bovine fetuses with subgroup 2, type 7 adenovirus. | three third trimester fetuses were exposed to a subgroup 2, type 7 adenovirus (adeno 7) by intraamnionic infection. the virus caused preterm delivery of two clinically ill calves and one stillbirth. the two premature calves died 12 and 72 hours after birth. an elevated serum neutralizing antibody titer (1:256) to adenovirus 7 was found in one principal calf at birth. adenovirus 7 was recovered from several tissues of the live calves and the spleen of the stillborn calf. fetuses exposed by intraa ... | 1978 | 217508 |
| respiratory viruses in hospital patients on the witwatersrand. a 7-year study. | virological investigations were carried out on 4 151 patients with respiratory disease hospitalized between may 1966 and april 1972. the groups examined were black and white children and black miners. influenza viruses were more common among malnourished black children and tended to cause more severe disease. this was also true of adenovirus and herpesvirus hominis type 1 infections. adenoviruses appear to be secondary invaders, frequently after a measles or influenza attack. a generalized epide ... | 1979 | 219551 |
| isolation of bovine adenovirus type 7 from calves with pneumonia and enteritis. | bovine adenovirus type 7 was isolated from a 10-month-old calf with fibrinopurulent pneumonia and from 2 newborn calves with pneumoenteritis. the viruses were isolated on calf lung and adrenal gland cell cultures and were identified as serotype 7 by immunoelectron microscopy and serum-neutralization tests. | 1978 | 219735 |
| extrapulmonary manifestations of adenovirus type 7 pneumonia simulating reye syndrome and the possible role of an adenovirus toxin. | three children developed extensive extrapulmonary disease in the course of fatal adenovirus type 7 pneumonia. several clinical features, including the unexpected onset of coma, suggested the development of reye syndrome, but biochemical and histopathologic findings were inconsistent with this diagnosis. virologic and pathologic studies did not reveal evidence of extrapulmonary adenovirus infection, despite clinical involvement of the liver, skeletal muscle, and central nervous system. the detect ... | 1979 | 224159 |
| sequence of inverted terminal repetitions from different adenoviruses: demonstration of conserved sequences and homology between sa7 termini and sv40 dna. | we have established the nucleotide sequence for the inverted terminal repetition of human adenovirus type 3, a subgroup b adenovirus. the repetition, which is 136 bp long, shows a high degree of homology with the known sequence for the inverted repetition of adenovirus type 5 (steenbergh et al., 1977) a subgroup c adenovirus. partial sequence information convering 120 bp of the inverted terminal repetitions of human serotype 12, a subgroup a member, and of simian adenovirus type 7 has also been ... | 1979 | 225041 |
| comparative sensitivity of a cultural test and the complement fixation test in the diagnosis of adenovirus ocular infection. | the sensitivity of human embryonic kidney (hek) cell culture and the complement fixation test (cft) in the diagnosis of adenovirus ocular infection has been compared. the optimum time for collection of specimens to obtain the best results by each test has been examined. sixty-one (53%) of 116 patients with moderate to severe follicular conjunctivitis, clinically indicative of adenovirus infection, had serological evidence of infection or had the virus isolated from ocular swabs. virus was isolat ... | 1979 | 226114 |
| evidence for an aetiological role for adenovirus type 7 in the mesenteric adenitis syndrome. | previous research has established the relationship between viral infection with adenovirus, influenza b virus and coxsackie b virus, and mesenteric adenitis. a prospective controlled survey of the incidence of viral infection in mesenteric adenitis was undertaken in children attending a children's hospital over a period of 13 months. eighteen patients, together with 18 controls who were matched for age, sex, and date of presentation, were studied. there were five cases of adenovirus type 7 infec ... | 1979 | 226850 |
| the inverted terminal repetition of the dna of weakly oncogenic adenovirus type 7. | by use of the chemical modification technique of maxam and gilbert (1977), the first 180 base pairs at both termini of the human adenovirus 7 genome have been determined. the results show that adenovirus 7 dna contains a perfect inverted terminal repetition of 136 base pairs. | 1979 | 231543 |
| [splitting and rejoining simian adenovirus type 7 dna by using eco ri restriction endonuclease and dna-ligase]. | optimal conditions for ligation of simian adenovirus type 7 (sa-7) dna fragments formed under the effect of treatment of the intact molecule with eco ri endonuclease were established. it was shown that up to 30% of the original material may be ligated and transferred into a structure with molecular weight 23 x 10(6) daltons which corresponded to the molecular weight of the intact sa-7 dna. the data of the existence of one recognition site for eco ri in sa-7 dna were confirmed. the biological act ... | 1977 | 333766 |
| genetic evidence for a temperature-sensitive lesion in the adenovirus 7 region of the para genome. | it has previously been shown that the helper adenovirus (ad) present in the defective ad 7-sv40 hybrid population is temperature-sensitive (ts) for replication in human cells. this study has shown that the replication of the defective hybrid virus (para) in green monkey kidney cells is also restricted at 40.5 percent. complementation tests between the parental or transcapsidant para populations and sv40 or various ad serotypes revealed that the ts lesion is located in the ad region of the hybrid ... | 1978 | 413806 |
| [fragmentation of dna of monkey adenovirus type 7 by specific endonucleases r. sali and r. bami]. | the effect of restrictive endonucleases r. ecor i, r. bam i, and r. sal i on simian adenovirus type 7 (sa7) genome was studied. because of the occurrence of the only recognition site, the r. ecor i enzyme cuts sa7 dna into two fragments with molecular mass of 12 and 10 x 10(6) daltons. r. ram i cuts this dna in 6 sites producing 7 fragments with molecular mass 6.6, 5.9, 3.8, 2.7, 1.3, 0.7 and 0.6 x 10(6) daltons. treatment with r. sal i gives 6 fragments with molecular mass of 8.1, 5.5, 4.3, 2.4 ... | 1978 | 417472 |
| [electron microscopic study of genome homology in 2 clones of the simian adenoviruses type 7]. | homology of the genomes of large-plaque (lp) and small-plaque (sp) clones of simian adenovirus type 7 (sv7) was studied experimentally. guanidine chloride helped to isolate circular dna molecules of identical length for both clones within the measurement error (1 = 11 +/- 0.2 micrometer). a heteroduplex sv7lp--sv7sp molecule was found to have two nonhomology sites 0.5 +/- 0.3 mu and 1.0 +/- 0.5 micrometer long located at a distance of 2 and 4 micrometer from one of the ends of the molecules, res ... | 1979 | 483773 |
| transformation of primary rat kidney cells by dna fragments of weakly oncogenic adenoviruses. | primary cultures of baby rat kidney (brk) cells were transformed by intact dna and dna fragments of weakly oncogenic human adenovirus types 3 and 7. the smallest fragment found to contain transforming activity was the left-terminal 4% endo r.hindiii fragment (for both adenovirus type 3 and 7 dnas). the efficiency of transformation of this fragment was low, and no permanent cell line could be established. left-terminal fragments ranging from 84 to 4,5% of the viral genome could all transform brk ... | 1979 | 513207 |
| [simian adenovirus type 7 (sa7) dna fragmentation by specific r.bgl ii and r.hind iii endonucleases]. | the effect of restricting r. bgiii and r. hindiii endonucleases on the genome of simian adenovirus type 7 (sa7) was studied. the r. bgiii endonuclease has 5 restriction sites on sa7 dna and produces 6 fragments with molecular weights of 10.0 x 10(6), 5,3 x 10(6), 3.6 x 10(6), 1.8 x 10(6), 0.6 x 10(6), and 0.5 x 10(6) daltons. r. hindiii, having 3 restriction sites, hydrolyses sa7 dna into 4 fragments with molecular weights of 7.6 x 10(6), 5.9 x 10(6), 5,2 x 10(6), 3,1 x 10(6) daltons. the r. bgi ... | 1979 | 524855 |
| mappings of adenovirus type 7 cytoplasmic rna species synthesized early in lytically infected cells and synthesized in transformed cells. | early virus-specific rna synthesized in kb cells infected with adenovirus type 7 and virus-specific rna synthesized in rat embryo cells (71jy1-2) transformed by the adenovirus type 7 hindiii-i.j fragment (left-hand 8.1% of the viral genome) have been mapped on the viral genome. about 25% of the viral genome, four discrete regions, two on each strand of the viral genome, are expressed as "early" mrna. almost similar regions in the left-hand 8.1% of the viral genome are transcribed both in kb cell ... | 1979 | 541826 |
| [characteristics of a culture of embryonal human lung cells spontaneously contaminated with mouse cells]. | the characteristics of human embryo lung culture spontaneously contaminated with mouse cells (helccm) are presented. changes in the cytological and karyological characteristics of the cells were observed as compared with those of the initial human embryo lung (hel) cell strain. the culture acquired the resistance to adenovirus type 7; at the same time rna metabolism in helccm culture culture chronically infected with adenovirus practically did not differ from that of the control. electron micros ... | 1978 | 567892 |
| transformation of a rat cell line by an adenovirus 7 dna fragment. | | 1978 | 676073 |
| localization of simian adenovirus 7 (sa 7) transcription and replication in lytic infection. an ultracytochemical and autoradiographical study. | we have studied sa7 (simian adenovirus 7) lytic infection at the ultrastruct level. the use of cytochemical techniques which specifically stain dna or preferentially stain ribonucleoproteins permitted the analysis of the structure of the virus-induced nuclear inclusions, and revealed presumed virus dna before the appearance of other nuclear alterations. correlation of these findings with high resolution autoradiography enabled the functions of virus dna replication and transcription to be ascrib ... | 1978 | 690607 |
| demonstration of three different subtypes of adenovirus type 7 by dna restriction site mapping. | restriction site mapping of the genomes of eight different isolates of adenovirus serotype 7 (ad7) has been performed with six different restriction endonucleases. in this analysis, 37 different restriction sites were localized. three distinctly different cleavage patterns of the genomes of the ad7 strains were observed. these strains could not be distinguished by serological techniques. the following three subtypes were defined on the basis of their restriction site patterns: the ad7 prototype, ... | 1978 | 711317 |
| defective virions of simian adenovirus 7 produced by one passage at high multiplicity of infection. | | 1978 | 726263 |
| ill defined neurological diseases of possible viral origin. | any approach towards elucidating the aetiology of an ill defined disease such as 'epidemic neuromyasthenia' has to be a comprehensive and wide-ranging one. although viruses must be strong candidates, by reason of their ubiquity, this need not necessarily be the case and we have recently seen the onset of legionnaires' disease as a new entity caused by a bacterium. we do not always recognize that a particular virus may affect the entire community and that the patient seen in hospital may represen ... | 1978 | 746022 |
| tumor-specific transplantation antigen(s) of bovine adenoviruses. | protection against bovine adenovirus type 3-induced primary or transplantable tumors was studied in hamsters immunized with bovine adenoviruses, human adenovirus type 12 (a-12), simian adenovirus type 7 (sa7), or chicken-embryo-lethal-orphan (celo) virus. newborn hamsters inoculated with 2.3 times 10-5 plaqueforming units of bovine adenovirus type 3 were given injections of bovine serotypes 1, 2, or 3 during the latent period of tumor development. no hamsters immunized with type 3 and only 47% o ... | 1975 | 803562 |
| the fate of type 7 adenovirions in lysosomes of hela cells. | properties of type 7 adenovirions in lysosomes of hela cells were studied 12 hr postinfection. viral particles were transferred to the lysosomes very quickly after initiation of penetration, i.e., after 10 min of incubation at 37 degrees. no morphological modification of the virion was detected for 6 hr postinfection. however, by 12 hr postinfection, the virion was no longer recognizable. most of the virus remained infectious for 2 hr, whereas after 12 hr the infectivity was abolished. soon afte ... | 1977 | 841871 |
| characterization of a temperature-sensitive mutant of human adenovirus type 7. | the properties of a naturally occurring temperature-sensitive (ts) mutant of human adenovirus type 7 (ad7) were studied. mutant ad7 (19), or e46-, was the nonhybrid adenovirus component derived from the defective simian virus 40 (sv40)-ad7 hybrid (para). growth of the mutant was restricted at 40.5 degrees c, and the ratios of virus yields in kb cells at 40.5 and 33 degrees c were 10(-2) to 10(-3). viral dna synthesis and the synthesis of adenovirus-specific antigens (tumor, capsid, hexon, and pe ... | 1977 | 850305 |
| deoxyribonuclease activity associated with adenovirus 5 and 7. | a dnase activity was found associated with isolated components of adenovirus types 5 and 7. the enzymatic activity was associated with the purified virus and viral components extracted from the soluble material of infected cells. the dnase activity of adenovirus type 5 was maximum at ph 5.5 and 7.0 whereas adenovirus 7 was active only at ph 7.0. both dnases were shown to be located in the penton fraction. | 1977 | 856583 |
| effect of pregnancy hormones on herpesvirus and other deoxyribonucleic acid viruses. | clinical impressions and observations of genital herpesvirus infections indicate that lesions are more persistent and more severe during pregnancy. several explanations are possible: an altered immune state, increased vascularity and blood flow, and/or increased viral infectivity due to increase in one or more hormones during pregnancy. studies presented here were directed toward investigating the latter possibility. herpesvirus, type 2, isolated from a genital lesion was titrated in wi-38 cells ... | 1977 | 900179 |
| viral dna sequences from incomplete particles of human adenovirus type 7. | large pools of empty viral capsids accumulate in cells infected by subgroup b human adenoviruses. such infected cells also yield dna-containing incomplete particles in larger quantities than cells infected with serotypes representing other adenovirus subgroups. dna isolated from carefully purified classes of ad7 incomplete particles was analyzed by restriction endonuclease cleavage, gel electrophoresis and electron microscopy. at least 90% of the dna molecules in each sample consisted of sequenc ... | 1977 | 902315 |
| cleavage maps of human adenovirus type 7 dna by restriction endonuclease hindiii and ecori. | | 1977 | 919350 |
| [characteristics of several indices of the mitotic regime of clone strains of hela cells infected with adenovirus type 7]. | the effect of adenovirus type 7 on the mitotic activity, the level and qualitative characteristics of pathological mitoses was studied in mycoplasma-free and latently mycoplasma-infected cells of clone hela cell lines with different sensitivity to this virus. the level and the extent of changes of the mitotic regimen in adenovirus-infected cells depend upon their susceptibility to the virus and initial characteristics of the mitotic regimen of the culture which is somehow related to the presence ... | 1976 | 983002 |
| in vitro transformation of rodent cells by simian adenovirus 7 and bovine adenovirus type 3 (strain wbr-1). | hamster, rat, and mouse embryo cells were quantitatively transformed by simian adenovirus 7 with maximum efficiencies of 2.1 x 10(5), and 2.5 x 10(5) plaque-forming unit/focus-forming unit, respectively. bovine adenovirus type 3 did not transform rat or mouse embryo cells, but transformed hamster embryo cells with a maximum efficiency of 3.4 x 10(4) plaque-forming unit/focus-forming unit. all of the transformed cells were tumorigenic in appropriate hosts and possessed viral-specific t-antigen. | 1976 | 999075 |
| detection of adenovirus in patient specimens by indirect immune electron microscopy. | immune electron microscopic procedures for the detection of adenovirus type 7 directly in throat swab specimens from patients are described. nineteen of 25 throat swab samples, known to be positive for adenovirus type 7 by isolation of virus from tissue culture, were shown to contain aggregates of adenovirions coated with antibody. sensitivity tests of the immune electron microscopic method showed that as few as 16 to 32 tissue culture infective doses of virus could be detected by the direct imm ... | 1975 | 1080765 |
| method for determination of nucleotide sequence homology between viral genomes by dna reassociation kinetics. | a model and appropriate equations were derived for the quantitative estimation of nucleotide sequence homology between two partially related viral genomes by measurement of the initial rate of reassociation of one labeled dna in the presence of a second unlabeled dna. the validity and usefulness of this procedure were demonstrated by the analysis of the reassociation kinetics of labeled adenovirus 7 dna in the presence of unlabeled adenovirus 2 dna. based on dna reassociation, the extent of homo ... | 1975 | 1113376 |
| unilateral hyperlucent lung with decreased vascular markings (swyer-james syndrome). | when diagnosing unilateral hyperlucency on a x-ray picture one of the possible underlying conditions is the swyer-james syndrome. it consists principally of hyperlucency, diminished size, deficient blood supply, decreased ventilation, and bronchiectasis of the affected lung. frequently a preceding pneumonia, caused by adenovirus, seems to be of aetiological importance. a yound patient, in whom the swyer-james syndrome was diagnosed on clinical, radiological, angiographical and ventilatory sympto ... | 1975 | 1124208 |
| recovery of adenovirus type 7 from human brain cell cultures. | a strain of adenovirus type 7 was recovered from cultured brain cells, taken at necropsy from a patient aged 71 years with chronic schizophrenia. this recovery may indicate the reactivation of a latent infection with one of the few adenoviruses that has regularly-if rarely-been associated with clinical encephalitis. | 1975 | 1159444 |
| initial safety and immunogenicity studies of an oral recombinant adenohepatitis b vaccine. | orally administered adenovirus may be a useful vaccine carrier of cloned antigens of other pathogens. a recombinant adenohepatitis vaccine wy-ad7hz6-1, which expressed hepatitis b surface antigen and contained a large deletion in early region 3 (e3), was constructed and studied in humans. volunteers received wy-ad7hz6-1 (n = 3), adenovirus type 7 vaccine (n = 3) or placebo (n = 3). recipients of wy-ad7hz6-1 shed less vaccine virus in the stool for a shorter period and had a lower titre of anti-a ... | 1992 | 1523878 |
| a rational system for classifying and denominating adenovirus genome types. | we propose to classify "genome types" of human adenoviruses in the same way as the "genomic clusters" defined by li and wadell, i.e. groups of closely related dna variants of a given serotype named by the use of indices, p (standing for prototype) a, b etc. variants within the genome type are named by letter-number indices (p1, p2; a1, a2 etc.). the percentage of comigrating restriction fragments and common restriction sites is significantly higher in variants than between genome types. the new ... | 1991 | 1647053 |
| sensitive analysis of genetic heterogeneity of adenovirus types 3 and 7 in the soviet union. | an analysis of adenovirus strains isolated in the soviet union from 1976 to 1988 revealed four genome types of adenovirus type 3 (ad3), i.e., ad3a4, ad3a9, ad3a10, and ad3a11, and four genome types of adenovirus type 7 (ad7), i.e., ad7p, ad7a, ad7a(1-5), and ad7f1, identified with the dna restriction enzymes bamhi, bglii, and hindiii. three of them, ad3a10, ad3a11, and ad7f1, are newly discovered. the genetic heterogeneity of adenoviruses was examined with restriction endonuclease cfr13i with a ... | 1991 | 1658038 |
| outbreak of adenoviral infections in a long-term paediatric facility, new jersey, 1986/87. | during an 8-week period in the winter of 1986-87, there were 11 deaths from an adenovirus infection (case fatality rate = 39%, 11/28) in a long-term care paediatric facility in southern new jersey. among the 61 resident children, all with severe congenital and/or acquired disabilities, 28 developed a febrile respiratory illness compatible with adenovirus infection [attack rate (ar) = 46%]. patients with tracheostomies were three times as likely to become ill [relative risk (rr) = 3.2, 95% confid ... | 1991 | 1680902 |
| co-expression of hepatitis b virus antigens by a non-defective adenovirus vaccine vector. | adenovirus type 7 vaccine strain was engineered to express foreign antigens from both the e3 early promoter in the e3 region and the major late promoter inserted between the e4 region and the right inverted terminal repeat. this multiple expression vector was used to express hepatitis b core antigen (hbcag), hepatitis b e antigen (hbeag), and hepatitis b surface antigen (hbsag). the gene inserted in the e3 region was derived from the core gene of the hepatitis b virus genome. when the precore re ... | 1991 | 1828660 |
| interaction of human embryonal carcinoma cells and differentiated derivatives in vitro with simian virus 40, human adenovirus type 7, or para. | polyclonal antibodies were used to assay human embryonal carcinoma (ec), differentiating ec, yolk sac carcinoma, and teratoma cells for expression of viral early antigen (t-ag) after infection with simian virus 40 (sv40). cells of four ec lines were induced to differentiate by cultivation at low density or by exposure to retinoic acid or dimethyl sulfoxide. after infection with sv40, t-ag was expressed by 1%, or less, of the cells (presumed to be differentiated derivatives) in only some ec cultu ... | 1990 | 2153554 |
| high level expression of the envelope glycoproteins of the human immunodeficiency virus type i in presence of rev gene using helper-independent adenovirus type 7 recombinants. | the effect of rev (art/trs) gene on the level of hiv-1 envelope (env) expression using recombinant adenovirus was investigated. recombinant adenoviruses expressing either the envelope or the rev gene of the human immunodeficiency virus type 1 (hiv-1) were constructed by inserting the gene into an expression cassette. the expression cassette contained the adenovirus type 7 major late promoter, followed by leader 1 of the adenovirus tripartite leader and a portion of intron between leaders 1 and 2 ... | 1990 | 2183470 |
| nucleotide and deduced amino acid sequence of the bovine adenovirus type 7 proteinase. | | 1990 | 2216744 |
| adenovirus type 7 infections in children in new south wales, australia. | this study describes our experience of adenovirus type 7 infection in children in new south wales, australia. some aspects of the epidemiology of the infection are also recorded. a community outbreak, a hospital-ward outbreak, sporadic cases, and data from a centralised registry are described. results of genome-type identification using restriction endonucleases are given. adenovirus type 7b infection was associated with significant mortality and points to the need, previously expressed, for an ... | 1989 | 2555443 |
| immunogenicity and efficacy testing in chimpanzees of an oral hepatitis b vaccine based on live recombinant adenovirus. | as a major cause of acute and chronic liver disease as well as hepatocellular carcinoma, hepatitis b virus (hbv) continues to pose significant health problems world-wide. recombinant hepatitis b vaccines based on adenovirus vectors have been developed to address global needs for effective control of hepatitis b infection. although considerable progress has been made in the construction of recombinant adenoviruses that express large amounts of hbv gene products, preclinical immunogenicity and eff ... | 1989 | 2570422 |
| insect virus: assays for toxic effects and transformation potential in mammalian cells. | the nuclear polyhedrosis virus of autographa californica (acnpv) was evaluated by using in vitro test systems for toxicity and transforming potential in mammalian cells. mass cell cultures of cv-1 and wi38 cells appeared unaffected by acnpv at a multiplicity of infection of 5. human foreskin cells grew more slowly after inoculation but eventually produced healthy monolayers. the sensitivities of the inhibition of reproductive survivability assays were greater and demonstrated slight acnpv toxici ... | 1989 | 2675761 |
| simian adenovirus type 7 (sa-7) induces tumours of nerve-supporting or paraneural cell origin in newborn hamsters. | simian adenovirus type 7 (sa-7) was found to induce tumours originating from nerve-supporting or paraneural cells in newborn hamsters, regardless of injection site or tissues. sa-7 induces glioblastomas characterized by definite localization (subependymal regions) and its main cell type, bipolar spongioblast-like cells, in the brain of hamsters inoculated as newborns. when the eyes of newborn hamsters were directly inoculated, sa-7 failed to induce retinoblastoma (0/27), but retro or peri-bulbar ... | 1989 | 2765394 |
| transformation of precrisis human cells by the simian virus 40 cytoplasmic-localization mutant psvct3 is accompanied by nuclear t antigen. | psvct3 is a cytoplasmic-localization mutant of simian virus 40 (sv40) isolated from the sv40 adenovirus 7 hybrid virus (para) and cloned into plasmid pbr. the large t antigen of psvct3 accumulates in the cytoplasm of infected monkey cells instead of being transported to the nucleus. the sole change in ct3 large t antigen is amino acid residue 128 (lys----asn). transformation of precrisis rodent cells by psvct3 is negligible, whereas the frequency of transformation of established rodent cell line ... | 1987 | 2822959 |
| adenovirus pneumonia. | adenoviruses are ubiquitous dna viruses that cause a wide variety of illnesses, including pneumonia, in children and adults. forty-one distinct human sero-types have been identified, yet only about six of these serotypes are associated with the majority of the cases of adenovirus-induced pneumonia. adeno-virus infection accounts for up to 20% of childhood pneumonias, primarily in those children younger than 5 years of age, but such pneumonias occur infrequently in the nonmilitary adult populatio ... | 1987 | 2827279 |
| two new genome types of adenovirus 7c. | adenovirus type 7 is the type most frequently associated with serious disease. eighteen different genome types of adenovirus type 7 had been reported up to october 1986. the genome type ad7c, based on the restriction enzyme profiles of smai and bamhi, has been reported from europe prior to 1969 and more recently from south africa. here, we report two new genome types of adenovirus 7 c that have not previously been identified and that have been isolated in south africa between 1975 and 1986 from ... | 1988 | 2828528 |
| infection of human thymic epithelial cells by human cytomegalovirus and other viruses: effect on secretion of interleukin 1-like activity. | cultures of human thymic epithelial (te) cells are able to produce a interleukin 1 (il-1) like activity. this il-1 activity can be detected either using mouse thymocytes in a traditional il-1 assay, or using thymic lymphocytes obtained from cases of pediatric cardio-vascular surgery. production of il-1 activity by te cells was found to be maximal between 3 and 4 weeks after culture initiation. human thymocytes worked best as targets in an il-1 assay, when these cells were derived from donors you ... | 1988 | 2844452 |
| factors predisposing to abnormal pulmonary function after adenovirus type 7 pneumonia. | | 1985 | 2982334 |
| herpes simplex virus type 1 infection of endothelial, epithelial, and fibroblast cells induces a receptor for c3b. | we recently demonstrated that herpes simplex virus type 1 (hsv 1) induces a receptor on human umbilical vein endothelial cells for complement component c3b (c3br). we assigned this receptor function to hsv 1 viral glycoprotein c (gc) based on several observations: tunicamycin, which prevents glycosylation and expression of n-linked glycoproteins on the surface of infected cells, markedly reduced expression of the c3br; monoclonal antibodies to hsv 1 gc blocked detection of the c3br, whereas mono ... | 1985 | 2982950 |
| partial sequence homologies between cytoskeletal proteins, c-myc, rous sarcoma virus and adenovirus proteins, transducin, and beta- and gamma-crystallins. | computer based sequence comparisons indicate partial sequence homology between human c-myc, rous sarcoma virus, adenovirus 7, and simian sarcoma virus proteins and the cytoskeletal proteins d9smin, keratin and vimentin. in addition, sections of the oncogene proteins showed partial but significant homology to alpha and gamma subunits of transducin. gamma-ii and beta-bp crystallins showed partial but significant homology to the cytoskeletal proteins keratin, vimentin, desmin, alpha and beta-tubuli ... | 1985 | 2985144 |
| adenovirus type 8 keratoconjunctivitis--an outbreak and its treatment with topical human fibroblast interferon. | an outbreak of keratoconjunctivitis is described which involved at least 186 people; adenovirus type 8 was identified in 50 of the cases. topical human fibroblast interferon was assessed in a double-blind, placebo-controlled study in which 34 patients participated. seventeen of the 34 trial patients yielded adenovirus type 8; three were infected with adenovirus type 7. the outbreak was curtailed by control of infection measures: principally careful hand-washing by medical personnel between cases ... | 1986 | 3016080 |
| analysis of 15 different genome types of adenovirus type 7 isolated on five continents. | a total of 15 different genome types of adenovirus type 7 (ad7), i.e., ad7p, ad7p1, ad7a, ad7a1 to ad7a5, ad7b, ad7c, ad7d, ad7d1, ad7e, ad7f, and ad7g, were identified among 40 selected strains isolated in europe, asia, north america, south america, and australia by using restriction endonucleases bamhi, bcli, bgli, bglii, bsteii, ecori, hindiii, hpai, sali, smai, xbai, and xhoi. eight of them, ad7p1, ad7a1 to ad7a5, ad7d1, and ad7g, are newly discovered. all 15 genome types could be distinguis ... | 1986 | 3018298 |
| genomic organization of the simian virus 40-adenovirus 7 hybrid virus, para(ct), that encodes a nuclear transport defective simian virus 40 t antigen. | the genomic organization of the simian virus 40 (sv40)-adenovirus (ad)7 hybrid virus, para(ct), was examined. a deletion of approximately 5529 bp of ad7 dna extends from 78.8 map units to 94.0 map units and is replaced by an sv40 dna insert of 3809 bp. the left-hand end of the insertion begins at sv40 nucleotide 5168, 5 bp upstream of the atg initiation codon for t-ag synthesis. the sequence extends counterclockwise through the t-ag encoding sequences and into sv40 late region dna. most of the l ... | 1986 | 3022475 |
| [effectiveness of incorporation of spermine-condensed viral dna into liposomes. interaction of liposomes with cells]. | dna from bacteriophage lambda or monkey adenovirus type 7 have been condensed with spermine and included into three types of liposomes: ethereal, obtained by inverted phases technique and by ca++ fusion. compact dna form presents a tor with 100 nm external diameter and 430 nm width. it can be included into 100 nm or larger liposomes. total preparation contains 15-18% of the required liposomes. liposomal fractions with included dna were separated from empty liposomes by step gradient of ficoll 40 ... | 1985 | 3025690 |
| isolation and characterization of temperature-sensitive mutants of adenovirus type 7. | fifty temperature-sensitive mutants, which replicate at 32 degrees c but not at 39.5 degrees c, were isolated after mutagenesis of the vaccine strain of adenovirus type 7 with hydroxylamine (mutation frequency of 9.0%) or nitrous acid (mutation frequency of 3.8%). intratypic complementation analyses separated 46 of these mutants into seven groups. intertypic complementation tests with temperature-sensitive mutants of adenovirus type 5 showed that the mutant in complementation group a failed to c ... | 1987 | 3041031 |
| the nucleotide sequence of the early region of the tupaia adenovirus dna corresponding to the oncogenic region e1b of human adenovirus 7. | the nucleotide sequence of the early region e1b of the tree shrew (tupaia) adenovirus (tav) dna has been determined. the sequenced region includes the genes for polypeptides of mr 15 000, 44 000 and 13 400, which are analogous to the small and large e1b proteins and protein ix, respectively, of the three human adenovirus serotypes 5, 7, and 12. the hexanucleotide consensus signal aataaa occurs only at the 3' terminus of the gene for protein ix suggesting that the e1 region of tav encompasses one ... | 1985 | 3159623 |
| characterization of a low-molecular-weight virus-associated (va) rna encoded by simian adenovirus type 7 which functionally can substitute for adenovirus type 5 va rnai. | human adenoviruses (ads), like ad type 2 (ad2) and ad5, encode a low-molecular-weight rna (designated virus-associated [va] rnai) which is required for the efficient translation of viral mrnas late after infection. we cloned and characterized a va rna gene from simian adenovirus type 7 (sa7) which appears to have biological activity analogous to that of ad2 va rnai. thus, sa7 va rna stimulates protein synthesis in a transient expression assay and can also functionally substitute for va rnai duri ... | 1986 | 3773054 |
| replication of human adenoviruses in guinea pig embryo cells: an ultrastructural study. | guinea pig embryo (gpe) cells showed different degrees of susceptibility to human adenovirus types as determined by virus infectivity assay and electron microscopic examination. adenovirus 2 and 5 induced extensive cellular changes and produced high titers of infectious virus in gpe cells as in human cells. mature progeny virus and protein crystals were observed in both cell types. adenovirus 7 induced some cellular changes in gpe cells but only a small number of cells yielded progeny virus as d ... | 1987 | 3827599 |
| e1a regions of the human adenoviruses and of the highly oncogenic simian adenovirus 7 are closely related. | simian adenovirus 7 (sa7) is a highly oncogenic virus, capable of causing tumors in hamsters upon the direct injection of viral dna. we determined the transcriptional organization of the transforming region and compared it with that of the human adenoviruses. this analysis demonstrated that there are two independently promoted transcription units similar to the e1a and e1b regions of the human adenoviruses. the nucleotide sequence of the sa7 e1a region demonstrated considerable homology with the ... | 1985 | 3968719 |
| molecular epidemiology of adenoviruses: global distribution of adenovirus 7 genome types. | adenovirus 7 (ad7) is the adenovirus species that most frequently has been associated with severe illness. seven distinct genome types of adenovirus 7, ad7p, ad7a, ad7b, ad7c, ad7d, ad7e, and ad7f, can be identified by using restriction endonucleases bamhi and smai. we analyzed the distribution of the different ad7 genome types among 314 isolates from patients and healthy shedders. the ad7b and ad7c genome types accounted for 90% of the isolates from patients and appeared to be mutually exclusiv ... | 1985 | 3980690 |
| comparative evaluation of three mammalian cell transformation assay systems. | one cellular transformation assay system (syrian hamster embryo (she) primary cells) and two viral mediated transformation systems (primary syrian hamster embryo cells infected with simian adenovirus type 7 (she/sa7), and a rat fibroblast cell line (2fr450) infected with rauscher leukemia virus (rat/rlv] were evaluated using a group of nine "model" and five coded chemicals. the purpose of the project was to establish the intra- and interlaboratory reproducibility of the assays and to provide a b ... | 1985 | 4053085 |
| immunofluorescence of simian adenovirus 7 (sa7)--specific antigens in infected llc-mk 2 cells and sa7 transformed cells. | | 1973 | 4120930 |
| transformation potentials of the noninfectious (defective) component in pools of adenoviruses type 12 and simian adenovirus 7. | pools of adenovirus 12 and simian adenovirus 7 were separated into four or five fractions by density gradient centrifugation in cesium chloride. each fraction was analyzed for total in vitro infectivity units, total transformation activity, and for total virus particle (vp) content. two major subpopulations were separated with mean densities of 1.30 +/- 0.02 and 1.34 +/- 0.02 g/ml, respectively. virions in the 1.34 g/ml range were highly infectious (10(2) to 10(3) vp per infectivity unit) in con ... | 1974 | 4211167 |
| immunofluorescent studies of the potentiation of an adenovirus-associated virus by adenovirus 7. | a quantitative immunofluorescent procedure for detection of viral antigen was used to study the potentiation of aav-1 by ad.7. aav viral antigen formed only when the cells were also infected with adenovirus, and only in cell culture systems in which the adenovirus infection proceeded to completion. ad. 7 infection of agmk. cell cultures did not potentiate aav unless the ad. 7 infection was itself potentiated by sv40. dose-response studies indicated that a single aav particle and a single infecti ... | 1967 | 4225814 |
| encephalitis associated with adenovirus type 7 occurring in a family outbreak. | | 1966 | 4285229 |
| [on two fatal cases of lung disease correlated with adenovirus type 7]. | | 1965 | 4285319 |
| ependymomas produced in syrian hamsters by adenovirus 7, strain e46 ("hybrid" of adenovirus 7 and sv40). | | 1965 | 4285456 |
| replication in simian cells of defective viruses in an sv40-adenovirus "hybrid" population. | butel, janet s. (baylor university college of medicine, houston, tex.), and fred rapp. replication in simian cells of defective viruses in an sv40-adenovirus "hybrid" population. j. bacteriol. 91:278-284. 1966.-an sv40-adenovirus type 7 "hybrid" virus population, previously shown to contain two viruses capable of complementation in green monkey kidney (gmk) cells, has a growth cycle in gmk cells similar to that of adenovirus type 7 in the presence of sv40. extending previous preliminary results, ... | 1966 | 4285535 |
| tumors induced in hamsters by a strain of adenovirus type 3: sharing of tumor antigens and "neoantigens" with those produced by adenovirus type 7 tumors. | | 1965 | 4285931 |