| canine adenovirus type 2-induced immunity to two canine adenoviruses in pups with maternal antibody. | twenty-four beagle pups with high levels of maternal antibody to canine adenovirus type 1 (cav-1) and canine adenovirus type 2 (cav-2) were oronasally inoculated with cav-2 at 4 weeks of age. the cav-2 was isolated from pharyngeal swabs on postinoculation days 2 through 6. in spite of the infection, maternal antibody continued to decrease for 4 to 8 postinoculation weeks, and then homologous cav-2 neutralizing antibody and, to a lesser extent, cav-1 neutralizing antibody began to increase. when ... | 1975 | 168797 |
| cross-protective immunity to canine adenovirus type 2 by canine adenovirus type 1 vaccination. | | 1978 | 216291 |
| viral-antibody complexes in canine adenovirus type 1 (cav-1)ocular lesions: leukocyte chemotaxis and enzyme release. | canine adenovirus-type 1 (cav-1)-antibody complexes caused severe anterior uveitis with corneal edema ("blue eye") when injected into the anterior chamber of normal dogs. the response of the anterior uvea to such immune complexes (ic) was similar to the spontaneously occurring disease. in the presence of complement (c'), ic caused release of neutrophile chemotactic factors. following phagocytosis of ic-c' leukocytes released lysosomal enzymes, as indicated by the presence of acid phosphatase in ... | 1975 | 1095299 |
| corneal endothelium in viral induced anterior uveitis. ultrastructural changes following canine adenovirus type 1 infection. | dogs inoculated intravenously with attenuated canine adenovirus type 1 developed anterior segment inflammation and corneal edema. during the stage of mild anterior uveitis, virus was isolated from the aquious fluid, and by electron microscopy, viral replication was found to occur in corneal endothelial cells. later, at the stage of severe anterior uveitis with corneal edema, virus was not isolated from the aqueous fluid and cells containing intranuclear (replicating) virus were not found. at thi ... | 1975 | 1138688 |
| nucleotide and deduced amino acid sequence of the canine adenovirus type 1 proteinase. | the dna sequence of an open reading frame (orf) corresponding to the canine adenovirus type 1 (can1) proteinase gene was determined. a total of 1171 base pairs were sequenced from the downstream end of the hindiii-a can1 genomic fragment, including adjacent regions corresponding to the carboxy-terminal portions of the hexon and the dna-binding proteins. the predicted can1 proteinase consists of 206 residues (23,325 d) of which 68% are identical and 83% are similar to the sequence of the human ad ... | 1992 | 1413543 |
| differences in the e3 regions of the canine adenovirus type 1 and type 2. | sequence analysis of a region extending between the pviii and the fiber gene of canine adenovirus type 1 (cav-1, utrecht) and type 2 (cav-2, manhattan) was performed. the results revealed a high level of identity between the two viruses when the pviii gene and the n-terminal part of the fiber gene were compared. the open reading frames of region e3 in of cav-1 and cav-2 encoded a 22 kda and 40.7 kda polypeptide, respectively. the cav-1 and cav-2 e3 region polypeptides shared conserved amino and ... | 1992 | 1534956 |
| prerequisites for oral immunization of free-ranging raccoons (procyon lotor) with a recombinant rabies virus vaccine: study site ecology and bait system development. | a model baiting system suitable for the delivery of an oral rabies vaccine to free-ranging raccoons (procyon lotor) was developed and tested on barrier islands in south carolina (usa). features of barrier island physiography and ecology were studied relative to selective bait deployment and site biosecurity. capture-mark-recapture data were obtained from 228 raccoons. raccoon density estimates, using a modified census assessment technique, were one raccoon per 1.8 to 2.7 ha. mean (+/- se) and ra ... | 1992 | 1548804 |
| antibody response to modified-live canine adenovirus vaccine in african hunting dogs (lycaon pictus). | nine hunting dogs were bled pre and post vaccination with modified-live virus vaccine. their antibody responses to canine adenovirus type 1 was measured by an indirect immunofluorescent antibody test. the vaccine proved to be both effective and safe. | 1991 | 1659069 |
| sequence analysis of putative e3 and fiber genomic regions of two strains of canine adenovirus type 1. | the nucleotide sequences of the genomes of two strains of canine adenovirus, type 1 (glaxo and cll) have been determined within the region spanning approximately 0.80-0.91 map unit. homology comparisons with other adenoviral dnas indicate that this region contains part of the hexon-associated protein (pviii) precursor gene on the left, and a 57-kda fiber-encoding gene on the right. in both viral strains, the sequences of these genes are identical. accepting the generally highly conserved nature ... | 1991 | 1828920 |
| [purification and properties of the hexon antigen of canine adenovirus serotype cav-1]. | the main antigen and immunogen of canine adenovirus type 1 (cav-1) has been purified to near homogeneity from cultural fluid of a cav-1-infected primary cell culture by hydrophobic and anion-exchange chromatography. the hexon native form (trimer) was shown to be resistant against denaturation by sds under conditions of sds-page performed without heating the samples. the monomer chain of the cav-1 hexon was apparently identical in terms of electrophoretic mobility with that of the previously sequ ... | 1990 | 2085617 |
| vaccine-associated canine distemper infection in a litter of african hunting dogs (lycaon pictus). | four, 57 days old, african hunting dog puppies (lycaon pictus) from one litter died within three weeks following vaccination with modified-live canine distemper virus (cdv) and killed canine adenovirus type 1, canine parvovirus and leptospira icterohemorrhagiae and canicola. 18 days post vaccination, the animals developed neurologic disease characterized by episodes of grand mal seizures and circling. macroscopic, histological and immunohistochemical studies revealed acute systemic cdv infection ... | 1990 | 2385981 |
| effects of vaccines on the canine immune system. | the effects of several commercially available polyvalent canine vaccines on the immune system of the dog were examined. the results demonstrated that the polyvalent vaccines used in this study significantly suppressed the absolute lymphocyte count and that most of the polyvalent vaccines significantly suppressed lymphocyte response to mitogen, but had no effect on natural effector cell activity, neutrophil chemiluminescence, nor antibody response to canine distemper virus. the individual vaccine ... | 1989 | 2540897 |
| identification and nucleotide sequence of the early region 1 from canine adenovirus types 1 and 2. | the genome of canine adenovirus type 1 (cav-1) has been cloned and restriction maps compiled. these maps are compared with those of canine adenovirus type 2 (cav-2). the left ends of both genomes were further characterised by dna sequence analysis. several features of the dna sequence and predicted polypeptide sequence are similar to those of the human adenoviruses. the level of homology observed across the e1 regions appears to be of the same order as the overall dna similarity between cav-1 an ... | 1989 | 2623943 |
| incomplete particles of canine adenovirus type 1: occurrence and structure. | replication of canine adenovirus (cav) type 1 in dog kidney (dk) cells has been found to generate particles containing double-stranded dna molecules of size 6-9 kbp with protein moieties at their 5' termini. the particles formed a discrete band in cscl gradients at a density intermediate to that of intact virions and empty capsids. electron microscopy demonstrated that they were morphologically similar to intact virions. restriction enzyme digests and hybridization experiments revealed the exist ... | 1989 | 2815582 |
| characterization of canine adenovirus type 1 isolated from american black bears. | viruses recovered from tissues taken at necropsy from american black bears were examined by use of immunofluorescence with polyclonal and monoclonal antibodies, virus neutralization with monoclonal antibodies, and restriction endonuclease analyses of the viral genomes. with these techniques, viruses were determined to be canine adenovirus type 1. seronegative dogs that were inoculated with the virus had clinical signs typical of infectious canine hepatitis, suggesting that the virus, which was v ... | 1988 | 2840839 |
| characterization of the genome of a vaccine strain of canine adenovirus type 1. | restriction endonuclease cleavage maps have been constructed for the genome of a canine adenovirus type 1 (cav-1) vaccine strain (cll; connaught laboratories, ltd., willowdale, ontario). restriction enzyme analyses were also carried out on cav-1 (cll) genomes isolated from viral stocks over 8 serial passages in a dog kidney cell line (dk 6722). the right hand 20% of the genome became more heterogeneous in size with increasing passage in dk 6722 cells due to deletions up to 3-4 kb, whereas the le ... | 1988 | 2852417 |
| two distinctive transforming dna regions on the canine adenovirus type 1 genome. | by transfecting cloned dna fragments of canine adenovirus type 1, a novel transforming region of mammalian adenovirus was demonstrated inside the viral genome. besides the left-end smai-d fragment (left 17.2%), an internally located smai-e fragment (17.2-28.7%) of canine adenovirus type 1 was found to transform primary rat kidney cells. | 1985 | 2991059 |
| comparative analysis of the canav-1 and canav-2 genomes. | the molecular weights of canine adenovirus type 1 (canav-1, strain utrecht) and type 2 (canav-2, strain toronto a26/61) dnas were determined by contour length measurements and restriction endonuclease analysis. in each case, an average molecular weight value of 20 (+/- 0.5) x 10(6) daltons was obtained with both methods. similar in size, the canav-1 and canav-2 dna molecules were, however, cleaved at very distinct positions by a variety of restriction endonucleases. the observed lack of dna sequ ... | 1986 | 3032842 |
| multiple reiteration of a 40-bp nucleotide sequence in the inverted terminal repeat of the genome of a canine adenovirus. | the dna of a vaccine strain of canine adenovirus type 1 [ichv vaccine; connaught laboratories, ltd.; cav-1(cll)] has been cloned in plasmid pat153 in the form of subgenomic bamhi digestion fragments. analysis of the nucleotide sequences of cloned terminal fragments has revealed an inverted terminal repeat (itr) with a minimum length of 198 nucleotides, including a tandem reiteration of the 40-bp nucleotide sequence from positions 14 to 53. the itrs had the 5'-catcatcaat ... sequence typical of a ... | 1987 | 3037784 |
| serum antibody response to canine parvovirus, canine adenovirus-1, and canine distemper virus in dogs with known status of immunization: study of dogs in sweden. | serum antibody titers to canine parvovirus (cpv), canine adenovirus-1 (cav-1), and canine distemper virus (cdv) were measured in dogs with known immunization status. the dogs represented 3 groups: nonvaccinated dogs less than 12 months old; vaccinated dogs less than 12 months old; and adult dogs greater than 12 months old. for practical reasons, the population from which the specimens were obtained could be considered as free from natural infection with cav-1 and cdv. in nonvaccinated dogs less ... | 1988 | 3223653 |
| monoclonal antibodies to canine adenoviruses 1 and 2 that are type-specific by virus neutralization and immunofluorescence. | monoclonal antibodies were produced against the mirandola strain of canine adenovirus type 1 (cav-1) and the manhattan strain of canine adenovirus type 2 (cav-2). the monoclonal antibodies were used in vitro in virus neutralization (vn) assays and in indirect fluorescent antibody (ifa) tests to examine several strains of each virus type. out of 36 monoclonal antibodies produced against the mirandola strain, 18 were type-specific for cav-1 by ifa and 13 of those neutralized the virus in vitro. th ... | 1988 | 3281371 |
| should the criteria for species distinction in adenoviruses be reconsidered? evidence from canine adenoviruses 1 and 2. | four strains of canine adenovirus type 1 (cav-1) and two strains of canine adenovirus type 2 (cav-2) were examined by restriction enzyme analysis. in all cases, cav-1 could be readily differentiated from cav-2. additionally, monoclonal antibodies were prepared against the mirandola and manhattan strains of cav-1 and cav-2, respectively. 18 of 36 monoclonal antibodies from the cav-1 fusion and 77 of 160 monoclonal antibodies from the cav-2 fusion were type-specific by an indirect fluorescent-anti ... | 1985 | 3884549 |
| canine viruses and multiple sclerosis. | serum and cerebrospinal fluid from multiple sclerosis (ms) patients and control subjects were tested and compared for presence and titer of neutralizing antibody against the most common canine viruses. canine viruses included canine distemper virus (cdv), canine adenovirus 1 (cav-1), canine parainfluenza virus (cpiv), canine herpesvirus (chv), canine coronavirus (ccv), and canine parvovirus (cpv). neutralizing titers against measles virus (mv) and human adenovirus 8 (ha8) were also tested. signi ... | 1981 | 6267515 |
| the 'blue eye' phenomenon. | diffuse clouding of the cornea ('corneal oedema', 'blue eye') of sudden onset and usually transient duration and with accompanying anterior uveitis, may be attributable to natural infection with canine adenovirus type 1 (ca-1) or to vaccination with live modified virus. it is now recognised that kerato-uveitis is a manifestation of type iii hypersensitivity in which immune complex formation resulting from the release of virus, especially from infected corneal endothelial cells, brings about corn ... | 1983 | 6304988 |
| unambiguous typing of canine adenovirus isolates by deoxyribonucleic acid restriction-endonuclease analysis. | viral deoxyribonucleic acid extracted from a limited number of cells infected with canine adenovirus type 1 or type 2 was cleaved with several restriction endonucleases. agarose gel electrophoresis of the limit digests showed stable differences between the canine adenovirus type 1 and type 2 cleavage patterns. rapid and accurate typing of large numbers of clinical isolates may thus be done by deoxyribonucleic acid restriction-endonuclease analysis. | 1983 | 6321002 |
| interspecific differences between the dna restriction profiles of canine adenoviruses. | identification of canine adenovirus-1 ( cav -1) and canine adenovirus-2 ( cav -2) strains was done by electrophoresis of restriction endonuclease-fragmented viral dna. results obtained with this sensitive and reproducible technique clearly show that cav -2 is not a variant of cav -1 but a distinct virus. | 1984 | 6327360 |
| dual infection with canine distemper virus and infectious canine hepatitis virus (canine adenovirus type 1) in a dog. | a 72-day-old, female, golden retriever dog showed anorexia, coughing, nasal discharge, diarrhea and hematochezia, and died on the 15th clinical day. pathological examination revealed dual infection with canine distemper virus (cdv) and canine adenovirus (cav). cav inclusion bodies occurred only in the liver, and biliary and respiratory system, whereas cdv inclusions were demonstrated in the visceral organs systematically. the cav inclusions were associated with multifocal hepatocellular necrosis ... | 1993 | 8399762 |
| primary structure of the canine adenovirus pvii protein: functional implications. | the dna sequence of an open reading frame (orf) corresponding to the canine adenovirus type 1 (can 1, utrecht strain) pro-vii (pvii) protein gene was determined. 560 base pairs were sequenced from the upstream end of the hindiii-a genomic fragment. the sequence gives the 33 c-terminal residues of the penton base protein followed by the 132 residue pvii protein, thus conforming to the same order and location of these genes as in ad2. the authenticity of this putative pvii-orf was confirmed by n-t ... | 1993 | 8460501 |
| infectious canine hepatitis: detection of canine adenovirus type 1 by polymerase chain reaction. | a primer pair and a polymerase chain reaction (pcr) method earlier tested for the detection of human and animal adenoviruses were used to demonstrate the presence of canine adenovirus type 1 (cav-1) in tissue culture and clinical specimens. a simple procedure of sample preparation was elaborated making the pcr easily applicable in rapid confirmation of the diagnosis of infectious canine hepatitis. | 1996 | 8908749 |
| complete dna sequence of canine adenovirus type 1. | the complete dna sequence of a field strain of canine adenovirus type 1 was determined by sequencing random fragments of viral dna cloned into pbluescript. the virus has a genome of 30536 bp flanked by two identical 161 bp inverted terminal repeats. thirty orfs have been identified, based on genomic location or sequence identity with published adenoviruses. these are arranged into similar discrete regions found in the human adenoviruses. orfs in the late region show greatest identity with publis ... | 1997 | 9129661 |
| [sequencing of right terminal fragment of canine adenovirus type 1 vaccine strain dna]. | current studies show that adenoviruses are more advantageous as expression vectors than other viruses. canine adenovirus type 1 (cav-1) vaccine strain, cll (cannaught laboratory limited), as vectors will play more important role for the prophylaxis of zoonosis than human adenoviruses. studies on molecular biology of cav-1 or cll are necessary for construction of vectors of cll. in this paper, the right terminal of cll dna cleaved by hind ii was sequenced. authors found that it contained a 238 bp ... | 1996 | 9208578 |
| viral antibodies in coyotes from california. | prevalence of antibodies against canine parvovirus (cpv), canine distemper virus (cdv), and canine adenovirus type 1 (cav) were determined among 152 coyotes (canis latrans) at the naval petroleum reserves (nprc; california, usa) from 1985 to 1990. overall prevalence of antibodies to cpv, cdv, and cav was 66%, 37%, and 68%, respectively. prevalence of cpv and cdv varied significantly among years. antibody prevalence did not differ between sexes for any disease, but did vary significantly among ag ... | 1998 | 9577772 |
| seroprevalence of selected disease agents from free-ranging black bears in florida. | sera obtained from 66 free-ranging florida black bears (ursus americanus floridanus) from three geographic areas of florida (usa) between november 1993 and august 1995 were tested for antibodies to 13 disease agents. antibody prevalences were 3 positive of 37 tested (8%) coxiella burnetti, 37 of 66 (56%) toxoplasma gondii, 3 of 61 (5%) bluetongue virus/epizootic hemorrhagic disease virus (btv/ehdv), 4 of 66 (6%) canine adenovirus-type 1, 5 of 66 (8%) canine distemper virus (cdv), 10 of 62 (16%) ... | 1998 | 9706572 |
| use of polymerase chain reaction and immunohistochemistry for detection of canine adenovirus type 1 in formalin-fixed, paraffin-embedded liver of dogs with chronic hepatitis or cirrhosis. | to assess the possible involvement of canine adenovirus type 1 (cav-1) in naturally occurring cases of canine chronic liver disease, a polymerase chain reaction (pcr)-based assay was developed to detect a conserved region of the major core protein gene (pvii) of cav-1 in formalin-fixed, paraffin-embedded liver sections. results were compared with a standard avidin-biotin immunoperoxidase complex technique that detected cav-1 antigens using a commercial monoclonal anti-adenovirus antibody. sevent ... | 1998 | 9786518 |
| presence of antibodies to canine distemper virus, canine parvovirus and canine adenovirus type 1 in free-ranging jackals (canis adustus and canis mesomelas) in zimbabwe. | a survey of free-ranging jackals (canis adustus and canis mesomelas) in zimbabwe was conducted to determine the prevalence of serum antibodies to canine distemper virus (cdv), canine parvovirus (cpv) and canine adenovirus type 1 (cav-1). sera from 16 canis adustus and 22 canis mesomelas were collected from 1990 to 1993 from various regions of zimbabwe and assayed by means of immunofluorescent techniques. seroprevalence in c. adustus and c. mesomelas respectively were 50% and 63.6% for cdv, 12.5% ... | 1999 | 10631712 |
| cellular uptake and infection by canine parvovirus involves rapid dynamin-regulated clathrin-mediated endocytosis, followed by slower intracellular trafficking. | canine parvovirus (cpv) is a small, nonenveloped virus that is a host range variant of a virus which infected cats and changes in the capsid protein control the ability of the virus to infect canine cells. we used a variety of approaches to define the early stages of cell entry by cpv. electron microscopy showed that virus particles concentrated within clathrin-coated pits and vesicles early in the uptake process and that the infecting particles were rapidly removed from the cell surface. overex ... | 2000 | 10644365 |
| serologic survey of selected viral agents in recently captured wild north american river otters (lontra canadensis). | blood samples were collected from 64 wild north american river otters (lontra [lutra] canadensis) from northern and eastern new york state and analyzed for serologic evidence of exposure to selected viral agents during a 1995 1996 translocation program. no clinical signs of disease nor lesions suggestive of prior viral exposure were seen. titers were detected for antibodies against canine distemper virus, canine herpesvirus-1, and canine parvovirus-2 but not for antibodies against canine adenovi ... | 2000 | 10982127 |
| polymerase chain reaction analysis for viruses in paraffin-embedded myocardium from dogs with dilated cardiomyopathy or myocarditis. | to perform polymerase chain reaction (pcr) analysis on paraffin-embedded myocardium from dogs with dilated cardiomyopathy (dcm) and dogs with myocarditis to screen for canine parvovirus, adenovirus types 1 and 2, and herpesvirus. | 2001 | 11197551 |
| detection and differentiation of cav-1 and cav-2 by polymerase chain reaction. | canine adenovirus type 1 (cav-1) and type 2 (cav-2) can be categorized in the laboratory by haemagglutination and neutralization tests, but they are difficult to differentiate from each other in specimens, especially when infection occurs in the digestive tract. the object of this study was to develop a simple method of detecting and differentiating them. one pair of common primers was designed and synthesized according to the sequences of the e3 and flanking regions and a polymerase chain react ... | 2001 | 11214675 |
| expression of canine interferon-gamma by a recombinant vaccinia virus and its antiviral effect. | a recombinant vaccinia virus-expressing canine interferon (ifn)-gamma (vv/cifn-gamma) was constructed. in rabbit kidney (rk13) and canine a72 cells infected with vv/cifn-gamma, ifn activity was detected in the culture supernatants of both cell types. canine ifn-gamma was also detected in both cell extracts by western blot. the activity of the recombinant canine ifn-gamma in rk13 cells was higher than that in a72 cells. the vv/cifn-gamma could not grow in a72 cells at a low multiplicity of infect ... | 2001 | 11325466 |
| severe enteric disease in an animal shelter associated with dual infections by canine adenovirus type 1 and canine coronavirus. | an outbreak of dual infection in dogs with canine adenovirus type 1 (cav-1) and canine coronavirus (ccv) infection is reported in an animal shelter that comprised approximately 200 adults stray dogs and 30 puppies. twenty puppies died 7-8 days after the onset of the clinical signs (severe enteritis, leucopoenia, respiratory distress and dehydration). both cav-1 and ccv were isolated from tissue or swab samples. antibodies to ccv and, at high levels, to cav-1 also were detected in several puppies ... | 2001 | 11471849 |
| generation of e3-deleted canine adenoviruses expressing canine parvovirus capsid by homologous recombination in bacteria. | e3-deleted canine adenovirus type 1 (cav-1) was generated by homologous recombination in bacterial cells, using an antibiotic resistance marker to facilitate the recovery of recombinants. this marker was flanked by unique restriction endonuclease sites, which allowed its subsequent removal and the insertion of cassettes expressing the canine parvovirus capsid at the e3 locus. infectious virus was recovered following transfection of canine cells and capsid expression was observed by rt-pcr from o ... | 2002 | 11853396 |
| glomerulonephritis associated with simultaneous canine adenovirus-1 and dirofilaria immitis infection in a dog. | this article describes a case of glomerulonephritis and immunocomplex (igm, igg and c3c) deposition in the mesangium and basement membranes of a 2-year-old dog with canine viral hepatitis and dirofilariasis. the deposits observed in the mesangium were in the vicinity of cells with viral replication. however, no clear relationship was found between viral replication and the deposition of immunocomplexes in the glomerular capillary basement membranes, which may be the reason why these deposits hav ... | 2002 | 12121044 |
| [development of a canine adenovirus type 1 vaccine strain e3-deleted based expression vector]. | to evaluate canine adenovirus type 1 vaccine strain (cannaught laboratory limited, cll) as recombinant vaccine and gene transfer vector. | 2001 | 12905816 |
| duration of serologic response to five viral antigens in dogs. | to determine whether vaccinated dogs either remained seropositive or responded serologically to revaccination for 5 key viral antigens after extended periods since their last vaccination. | 2004 | 14710876 |
| diagnosis of infectious canine hepatitis virus (cav-1) infection in puppies with encephalopathy. | nine weaned labrador retriever puppies from a litter of 11 were presented with signs of acute central nervous system (cns) disease that included ataxia and blindness. all puppies died. gross examination of tissues from 2 puppies revealed regionally diffuse hemorrhages in the brain stem and swollen hemorrhagic lymph nodes. light microscopic examination of hematoxylin and eosin-stained tissues showed numerous large, basophilic intranuclear inclusion bodies within cns vascular endothelium and occas ... | 2005 | 15690952 |
| three-year duration of immunity in dogs following vaccination against canine adenovirus type-1, canine parvovirus, and canine distemper virus. | a challenge-of-immunity study was conducted to demonstrate immunity in dogs 3 years after their second vaccination with a new multivalent, modified-live vaccine containing canine adenovirus type 2 (cav-2), canine parvovirus (cpv), and canine distemper virus (cdv). twenty-three seronegative pups were vaccinated at 7 and 11 weeks of age. eighteen seronegative pups, randomized into groups of six dogs, served as challenge controls. dogs were kept in strict isolation for 3 years following the vaccina ... | 2005 | 15906266 |
| a human adenoviral vector with a chimeric fiber from canine adenovirus type 1 results in novel expanded tropism for cancer gene therapy. | the development of novel therapeutic strategies is imperative for the treatment of advanced cancers like ovarian cancer and glioma, which are resistant to most traditional treatment modalities. in this regard, adenoviral (ad) cancer gene therapy is a promising approach. however, the gene delivery efficiency of human serotype 5 recombinant adenoviruses (ad5) in cancer gene therapy clinical trials to date has been limited, mainly due to the paucity of the primary ad5 receptor, the coxsackie and ad ... | 2005 | 16034451 |
| infectious disease and the decline of steller sea lions (eumetopias jubatus) in alaska, usa: insights from serologic data. | serologic data were examined to determine whether infectious disease may have played a role in the decline of steller sea lions (eumetopias jubatus) in the gulf of alaska and aleutian islands, usa. available published data, unpublished data, and recent collections (1997-2000) were compared and reviewed. data were stratified by geography to compare the declining western alaskan population in the aleutian islands through eastern prince william sound to the increasing population in southeastern ala ... | 2005 | 16244061 |
| cloning and expression of canine interferon-alpha genes in escherichia coli. | we cloned five new subtypes of cdna encoding canine interferon-alpha (caifn-alpha) from a canine epithelial cell line. caifn-alphas were divided into two groups by amino acid sequences and a molecular phylogenic tree. two subtypes of them were expressed in escherichia coli, and ifn proteins were purified. recombinant caifn-alphas were highly species-specific and showed antiviral activity against vesicular stomatitis new jersey virus and canine adenovirus-1 , but not against canine herpesvirus-1. | 2005 | 16276065 |
| a serologic survey for antibodies to three canine viruses in wolverines (gulo gulo) from the brooks range, alaska. | canine distemper virus (cdv), canine parvovirus type 2 (cpv-2), and canine adenovirus type 1 (cav-1) are pathogens that are typically associated with canids but may cause serious disease in a wide range of other carnivores. from 1998 to 2002, serum samples from 64 wolverines (gulo gulo) from the brooks range, alaska, were tested for antibodies to cdv, cpv-2, and canine adenovirus (cav). four animals tested positive for antibodies to cdv (7%), one for antibodies to cpv-2 (2%), and none for antibo ... | 2005 | 16456170 |
| three-year rabies duration of immunity in dogs following vaccination with a core combination vaccine against canine distemper virus, canine adenovirus type-1, canine parvovirus, and rabies virus. | thirty-two seronegative pups were vaccinated at 8 weeks of age with modified-live canine distemper virus (cdv), canine adenovirus type-2 (cav-2), and canine parvovirus (cpv) vaccine and at 12 weeks with a modified-live cdv, cav-2, cpv, and killed rabies virus vaccine. an additional 31 seronegative pups served as age-matched, nonvaccinated controls. all test dogs were strictly isolated for 3 years after receiving the second vaccination and then were challenged with virulent rabies virus. clinical ... | 2006 | 17039445 |
| infectious canine hepatitis: an "old" disease reemerging in italy. | four outbreaks of infectious canine hepatitis (ich) occurring in italy between 2001 and 2006 are reported. three outbreaks were observed in animal shelters of southern italy, whereas a fourth outbreak involved two purebred pups imported from hungary few days before the onset of clinical symptoms. in all outbreaks canine adenovirus type 1 (cav-1) was identified by virus isolation and pcr. in three outbreaks, other canine viral pathogens were detected, including canine distemper virus, canine parv ... | 2007 | 17197003 |
| fiber-knob modifications enhance adenoviral tropism and gene transfer in malignant glioma. | malignant gliomas remain refractory to ad5-mediated gene therapy due to deficiency of the coxsackie adenovirus receptor on tumor cells. the purpose of this study was to evaluate whether changes in adenoviral tropism can enhance gene transfer in the context of malignant glioma. | 2007 | 17351980 |
| a serologic assessment of exposure to viral pathogens and leptospira in an urban raccoon (procyon lotor) population inhabiting a large zoological park. | in urban environments, raccoons (procyon lotor) may act as reservoirs for an array of pathogenic organisms, presenting spillover risks for human, domestic animal, and captive (zoo) animal populations. over 5 yr, 159 raccoons from a high-density raccoon population in st. louis, missouri (usa), were surveyed for exposure to canine distemper virus (cdv), canine adenovirus 1 (cav-1); feline parvovirus (fpv; =feline panleukopenia), and several serovars of leptospira interrogans. exposure to each of t ... | 2007 | 17469271 |
| canine adenovirus type 1 infection of a eurasian river otter (lutra lutra). | a 10-year-old female eurasian river otter (lutra lutra) died after prolonged anorexia and weight loss in the seoul grand park zoo, seoul, republic of korea. on necropsy, the liver was found to be swollen and friable with 1 lobe enlarged and necrotic. the other organs showed no significant alterations except for mild atrophy of the right kidney. microscopically, there was multifocal hepatic necrosis. the hepatocytes around the necrotic areas were swollen and contained large basophilic intranuclea ... | 2007 | 17606519 |
| augmented humoral and cellular immune responses induced by canine adenovirus type 1 dna vaccine in balb/c mice. | infectious canine hepatitis (ich) is caused by canine adenovirus type 1 (cav-1), which severely harms infected animals. vaccination provides an effective approach to preventing canine infectious diseases. with the objective of exploring a new vaccination strategy that may prevent or cure ich, we constructed a dna vaccine, pvax1-cpg-loop, and evaluated its immune efficacy. we found that vaccination of balb/c mice with the dna vaccine alone, or priming with dna vaccine and boosting with the loop p ... | 2007 | 17931116 |
| infectious canine hepatitis in a gray fox (urocyon cinereoargenteus). | a free-ranging adult male gray fox (urocyon cinereoargenteus) with moderate diarrhea and thick ocular mucus discharge was examined postmortem. microscopically, the fox had intranuclear inclusion bodies within hepatocytes. canine adenovirus-1 was identified by polymerase chain reaction (pcr) and nucleotide sequencing. to our knowledge, this is the first report of clinical infectious canine hepatitis in a gray fox. | 2007 | 17984271 |
| three-year serologic immunity against canine parvovirus type 2 and canine adenovirus type 2 in dogs vaccinated with a canine combination vaccine. | a group of client-owned dogs and a group of dogs at a commercial kennel were evaluated for duration of antibody responses against canine parvovirus type 2 (cpv-2) and canine adenovirus type 1 (cav-1) after receiving a combination vaccine containing recombinant canarypox-vectored canine distemper virus (cdv) and modified-live cpv-2, cav-2, and canine parainfluenza virus, with (c6) or without (c4) two serovars of leptospira (recombitek c4 or c6, merial). duration of antibody, which correlates with ... | 2007 | 18183549 |
| detection of canine adenoviral infections in urine and faeces by the polymerase chain reaction. | the present study was aimed at developing the polymerase chain reaction (pcr) assay for detection of canine adenoviruses from faecal or urine samples. urine or faecal samples were treated with chloroform or activated charcoal to eliminate the pcr inhibitory substances and the total dna was extracted. the pcr was optimized using common set of primers to amplify 508 bp or 1,030 bp dna sequence within e3 gene of canine adenovirus-1 (cav-1) and canine adenovirus-2 (cav-2), respectively. the pcr assa ... | 2008 | 18329729 |
| disease threats to the endangered iberian lynx (lynx pardinus). | the iberian lynx, (lynx pardinus), is the most endangered felid in the world. to determine whether sympatric carnivores are reservoirs of pathogens posing a disease risk for the lynx, evidence of exposure to 17 viral, bacterial and protozoan agents was investigated in 176 carnivores comprising 26 free-living lynx, 53 domestic cats, 28 dogs, 33 red foxes (vulpes vulpes), 24 egyptian mongooses (herpestes ichneumon), 10 common genets (genetta genetta) and 2 eurasian badgers (meles meles) in the are ... | 2009 | 18555712 |
| tyzzer's disease in a eurasian otter (lutra lutra) in scotland. | a eurasian otter (lutra lutra) cub found in weak condition on the isle of harris, scotland, developed bilateral corneal oedema 16 days after being admitted to a rehabilitation centre. it died unexpectedly on day 26. on postmortem examination, there was excess clear fluid in the body cavities and the liver was swollen with numerous pale focal lesions and petechial haemorrhages throughout. histopathological examination revealed bundles of bacilli morphologically typical of clostridium piliforme wi ... | 2008 | 18978367 |
| hematology, parasitology, and serology of free-ranging coyotes (canis latrans) from south carolina. | blood and feces were collected from 34 adult (19 males, 15 females) and seven juvenile (three males, one female, three not reported) free-ranging coyotes (canis latrans) on the us department of energy's savannah river site (south carolina, usa). significant (p<0.05) hematologic differences by sex were noted for red blood cell counts, hemoglobin, and hematocrit. biochemical differences by sex occurred only for albumen (p<0.05). twenty-one adults were antibody positive for at least one of four vir ... | 2009 | 19617502 |
| a serological survey of infectious disease in yellowstone national park's canid community. | gray wolves (canis lupus) were reintroduced into yellowstone national park (ynp) after a >70 year absence, and as part of recovery efforts, the population has been closely monitored. in 1999 and 2005, pup survival was significantly reduced, suggestive of disease outbreaks. | 2009 | 19756151 |
| age and long-term protective immunity in dogs and cats. | vaccination can provide an immune response that is similar in duration to that following a natural infection. in general, adaptive immunity to viruses develops earliest and is highly effective. such anti-viral immune responses often result in the development of sterile immunity and the duration of immunity (doi) is often lifelong. in contrast, adaptive immunity to bacteria, fungi or parasites develops more slowly and the doi is generally short compared with most systemic viral infections. steril ... | 2010 | 19959181 |
| infectious canine hepatitis in red foxes (vulpes vulpes) in the united kingdom. | the pathological findings are described in three cases of infectious canine hepatitis in free-ranging red foxes (vulpes vulpes) in england. the foxes died after short periods of clinical illness. mild jaundice and hepatic congestion were evident grossly. on histopathological examination, intranuclear inclusion bodies were visible in hepatocytes, in association with hepatocyte dissociation and necrosis, as well as in renal glomeruli, renal tubular epithelial cells and vascular endothelial cells. ... | 2010 | 20097890 |
| [infectious canine hepatitis in 4 dogs in switzerland]. | infectious canine hepatitis in 4 dogs in switzerland. four dogs presented with nonspecific symptoms of lethargy, vomiting, diarrhea, fever and weakness. laboratory results were consistent with hepatopathy and disseminated intravascular coagulation. three dogs died, one survived. in the three deceased dogs, a diagnosis of infectious canine hepatitis (ich) was made based on histological findings and positive immunhistochemistry results for canine adenovirus-1 (cav-1). in the surviving dog, an ante ... | 2010 | 20127647 |
| serosurvey for canine distemper virus, canine adenovirus, leptospira interrogans, and toxoplasma gondii in free-ranging canids in scandinavia and svalbard. | prevalence of antibodies reactive to canine distemper virus (cdv), canine adenovirus type 1 (cav-1), leptospira interrogans serovars canicola and icterohaemorrhagiae, and toxoplasma gondii were examined in free-ranging scandinavian canids. sampling included 275 red foxes (vulpes vulpes) from mainland norway, 60 arctic foxes (vulpes lagopus) from the high-arctic islands of svalbard, and 98 wolves (canis lupus) from the joint swedish-norwegian population. methods used included virus neutralization ... | 2010 | 20688639 |
| lights and shades on an historical vaccine canine distemper virus, the rockborn strain. | both egg- and cell-adapted canine distemper virus (cdv) vaccines are suspected to retain residual virulence, especially if administered to immuno-suppressed animals, very young pups or to highly susceptible animal species. in the early 1980s, post-vaccine encephalitis was reported in dogs from various parts of britain after administration of a particular batch of combined cdv rockborn strain/canine adenovirus type-1 vaccine, although incrimination of the rockborn strain was subsequently retracte ... | 2010 | 21167117 |
| plaque purification as a method to mitigate the risk of adventitious-agent contamination in influenza vaccine virus seeds. | at present, the seed viruses for the manufacture of licensed seasonal inactivated influenza vaccines in the united states are derived from primary egg isolates as a result of concerns associated with adventitious agents. according to the prevailing view, the passage of influenza viruses through eggs serves as a filtering step to remove potential contaminating viruses. we have investigated the feasibility of addressing adventitious-agent risk by subjecting influenza virus to a plaque-purification ... | 2011 | 21354480 |
| isolation of a novel adenovirus from california sea lions zalophus californianus. | viral hepatitis associated with adenoviral infection has been reported in california sea lions zalophus californianus admitted to rehabilitation centers along the california coast since the 1970s. canine adenovirus 1 (cadv-1) causes viral hepatitis in dogs and infects a number of wildlife species. attempts to isolate the virus from previous sea lion hepatitis cases were unsuccessful, but as the hepatitis had morphologic features resembling canine infectious hepatitis, and since the virus has a w ... | 2011 | 21790072 |
| chronic hepatitis in the english springer spaniel: clinical presentation, histological description and outcome. | medical records and liver histology of 68 english springer spaniels (ess) with a histological diagnosis of ch were reviewed retrospectively. pcr was performed on liver tissue for canine adenovirus-1 (cav-1), canine parvovirus, canine herpesvirus and pathogenic leptospira species. follow-up information was obtained to calculate survival times. median age at presentation was three years seven months (range, seven months to eight years five months) and there were 48 female and 20 male dogs. clinica ... | 2011 | 21852307 |
| antibody titers for canine parvovirus type-2, canine distemper virus, and canine adenovirus type-1 in adult household dogs. | serum antibody titers for canine parvovirus type-2 (cpv-2), canine distemper virus (cdv) and canine adenovirus type-1 (cav-1) were investigated in 1031 healthy adult household dogs (2 to 18 years old) given an annual inoculation in the previous 11 to 13 months. the number of dogs retaining significant titers of antibodies against cpv-2, cdv, and cav-1 were 888 (86%), 744 (72%), and 732 (71%), respectively. there were no differences between males and females in antibody titers against the 3 virus ... | 2011 | 22379198 |
| effects of body weight on antibody titers against canine parvovirus type 2, canine distemper virus, and canine adenovirus type 1 in vaccinated domestic adult dogs. | the objective of this study was to determine whether post-vaccination antibody titers vary according to body weight in adult dogs. antibody titers against canine parvovirus type 2 (cpv-2), canine distemper virus (cdv), and canine adenovirus type 1 (cadv-1) were measured for 978 domestic adult dogs from 2 to 6 y of age. the dogs had been vaccinated approximately 12 mo earlier with a commercial combination vaccine. the dogs were divided into groups according to their weight. it was found that mean ... | 2012 | 23543958 |
| health survey of free-ranging raccoons (procyon lotor) in central park, new york, new york, usa: implications for human and domestic animal health. | we conducted health assessments on 113 free-ranging raccoons ( procyon lotor ) in central park, new york city, us, in february 2010, september 2010, and november 2011 in conjunction with a trap-vaccinate-release program to control a raccoon rabies epizootic. five individuals were sampled at two time points for 118 raccoon examinations in total. we tested 13 of 13 and 8 of 13 euthanized raccoons for rabies and canine distemper virus (cdv), respectively, by antigen testing on brain tissue; all wer ... | 2017 | 28135131 |
| enteritis in an alpaca (vicugna pacos) associated with a potentially novel adenovirus. | adenovirus-associated enteritis was diagnosed by histopathology of small intestine in a 2-year-old alpaca (vicugna pacos). electron microscopy confirmed intracytoplasmic and intranuclear adenoviral particles within enterocytes. nucleic acid was extracted from paraffin-embedded tissue sections, and a pan-adenovirus nested polymerase chain reaction (pcr) assay was employed to target a partial sequence of the polymerase gene. the pcr product (321 bp) was cloned and sequenced. comparison of the nucl ... | 2012 | 22786976 |
| virus quantitation by transmission electron microscopy, tcid₅₀, and the role of timing virus harvesting: a case study of three animal viruses. | quantitation of viruses is practised widely in both basic and applied virology. infectious titration in cell cultures, the most common approach to it, is quite labour-intensive and alternative protocols are therefore sought. one of the alternatives is transmission electron microscope (tem) quantitation using latex particles at a known concentration as a reference for counting virus particles. if virus tcid₅₀ is determined in parallel, the ratio of infectious to non-infectious virus particles may ... | 2013 | 23603437 |
| patterns of exposure of iberian wolves (canis lupus) to canine viruses in human-dominated landscapes. | wildlife inhabiting human-dominated landscapes is at risk of pathogen spill-over from domestic species. with the aim of gaining knowledge in the dynamics of viral infections in iberian wolves (canis lupus) living in anthropized landscapes of northern spain, we analysed between 2010 and 2013 the samples of 54 wolves by serology and polymerase chain reaction (pcr) for exposure to four pathogenic canine viruses: canine distemper virus (cdv), canine parvovirus-2 (cpv), canine adenovirus 1 and 2 (cav ... | 2016 | 26589403 |
| establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. | although widespread vaccination against canine distemper virus (cdv) has been conducted for many decades, several canine distemper outbreaks in vaccinated animals have been reported frequently. in order to detect and differentiate the wild-type and vaccine strains of the cdv from the vaccinated animals, a novel reverse transcription loop-mediated isothermal amplification (rt-lamp) method was developed. a set of four primers-two internal and two external-were designed to target the h gene for the ... | 2015 | 25769803 |
| canine adenovirus type 1 in a fennec fox (vulpes zerda). | a 10-mo-old female fennec fox (vulpes zerda) with drooling suddenly died and was examined postmortem. histologic examination of different tissue samples was performed. vacuolar degeneration and diffuse fatty change were observed in the liver. several diagnostic methods were used to screen for canine parvovirus, canine distemper virus, canine influenza virus, canine coronavirus, canine parainfluenza virus, and canine adenovirus (cadv). only cadv type 1 (cadv-1) was detected in several organs (liv ... | 2014 | 25632689 |
| polymerase spiral reaction (psr): a novel, visual isothermal amplification method for detection of canine parvovirus 2 genomic dna. | canine parvovirus-2 (cpv-2), which is ubiquitously distributed worldwide, causes severe and often fatal gastroenteritis in dogs. accurate, differential and rapid diagnosis of canine parvoviral enteritis remains a challenge for clinicians. a recently developed isothermal amplification technique, polymerase spiral reaction (psr), was optimized for the first time for a viral pathogen with reference recombinant plasmid standards from different cpv-2 antigenic variants (cpv-2, cpv-2a, cpv-2b and cpv- ... | 2017 | 28349355 |
| high prevalence of antibodies against canine adenovirus (cav) type 2 in domestic dog populations in south africa precludes the use of cav-based recombinant rabies vaccines. | rabies in dogs can be controlled through mass vaccination. oral vaccination of domestic dogs would be useful in the developing world, where greater vaccination coverage is needed especially in inaccessible areas or places with large numbers of free-roaming dogs. from this perspective, recent research has focused on development of new recombinant vaccines that can be administered orally in a bait to be used as adjunct for parenteral vaccination. one such candidate, a recombinant canine adenovirus ... | 2013 | 23867013 |
| duration of serological response to canine parvovirus-type 2, canine distemper virus, canine adenovirus type 1 and canine parainfluenza virus in client-owned dogs in australia. | to determine whether client-owned dogs in australia, last vaccinated with canvac(®) vaccines containing canine parvovirus-type 2 (cpv-2), canine distemper virus (cdv), canine adenovirus type 2 (cav-2) ± canine parainfluenza virus (cpiv) at least 18 months ago, were seropositive or responded serologically to revaccination. | 2012 | 23186088 |
| booster effect of canine distemper, canine parvovirus infection and infectious canine hepatitis combination vaccine in domesticated adult dogs. | domesticated adult dogs with antibody titer classified as below 'high' to one or more of canine distemper virus (cdv), canine parvovirus type-2 (cpv-2) and canine adenovirus type-1 (cadv-1) were then given an additional inoculation, and the effectiveness of this booster evaluated 2 months later. consequently, cdv and cadv-1 antibody titer experienced a significant increase, but the same effect was not observed in the antibody titer of cpv-2. these findings suggest that with additional inoculatio ... | 2012 | 22672137 |
| serological and molecular epidemiology of canine adenovirus type 1 in red foxes (vulpes vulpes) in the united kingdom. | canine adenovirus type 1 (cav-1) causes infectious canine hepatitis (ich), a frequently fatal disease which primarily affects canids. in this study, serology (elisa) and molecular techniques (pcr/qpcr) were utilised to investigate the exposure of free-ranging red foxes (vulpes vulpes) to cav-1 in the united kingdom (uk) and to examine their role as a wildlife reservoir of infection for susceptible species. the role of canine adenovirus type 2 (cav-2), primarily a respiratory pathogen, was also e ... | 2016 | 27796367 |
| a duplex real-time pcr assay based on taqman technology for simultaneous detection and differentiation of canine adenovirus types 1 and 2. | canine adenoviruses are a major cause of disease in dogs, coyotes, red foxes and wolves, as well as in other carnivores and marine mammals. canine adenovirus type 1 (cadv-1) and canine adenovirus type 2 (cadv-2) cause infectious canine hepatitis (ich) and infectious tracheobronchitis (itb), respectively. in this study, a duplex real-time pcr assay for simultaneous detection and characterisation of cadv-1 and cadv-2 was developed by using a single primer pair and virus-specific probes. the assay ... | 2016 | 27040113 |
| canine adenovirus type 1 and pasteurella pneumotropica co‑infection in a puppy. | in 2008, a 2 months-old male german shepherd was presented with fever, depression, and evident organic wasting. the puppy died within 48 hours after the onset of clinical signs. a complete necropsy was performed. bacteriological examination of samples from the brain, lung, liver, spleen, and bone marrow tested positive for pasteurella pneumotropica. histopathology demonstrated inflammatory and vascular lesions in the central nervous system and internal organs. canine adenovirus type 1 nucleic ac ... | 2017 | 27033531 |
| infectious canine hepatitis in red foxes (vulpes vulpes) in wildlife rescue centres in the uk. | outbreaks of infectious canine hepatitis are described in red foxes ( italic! vulpes vulpes) at two wildlife rescue centres in the uk. disease occurred in two-month-old to four-month-old juvenile foxes, which were held in small enclosures in groups of three to eight animals. the foxes died or were euthanased after a short clinical course, sometimes including neurological signs and jaundice, with a high case fatality rate. four red foxes submitted for postmortem examination had enlarged, congeste ... | 2016 | 27001767 |
| development of a sybr green real-time pcr assay with melting curve analysis for simultaneous detection and differentiation of canine adenovirus type 1 and type 2. | canine adenovirus type 1 (cadv-1) and canine adenovirus type 2 (cadv-2) cause infectious canine hepatitis (ich) and infectious tracheobronchitis (itb) in dogs, respectively. cases of ich have been documented in recent years and recent surveys have demonstrated a wide percentage of asymptomatic cadv-1 infection in the canine population. since both cadv types are detectable in the same biological matrices, and viral coinfection with cadv-1 and cadv-2 are reported with high frequency, it is urgent ... | 2015 | 26028428 |
| the serological and virological investigation of canine adenovirus infection on the dogs. | two types of canine adenovirus (cavs), canine adenovirus type 1 (cav-1), the virus which causes infectious canine hepatitis, and canine adenovirus type 2 (cav-2), which causes canine infectious laryngotracheitis, have been found in dogs. in this study, blood samples taken from 111 dogs, which were admitted to the internal medicine clinic of selcuk university, faculty of veterinary medicine, with clinical symptoms. seventy-seven dogs were sampled from isparta and burdur dog shelters by random sam ... | 2013 | 24223508 |
| molecular epidemiology of canine adenovirus type 1 and type 2 in free-ranging red foxes (vulpes vulpes) in italy. | to date, no studies exist regarding the presence of canine adenovirus (cadv) infection in foxes in italy. furthermore, the majority of worldwide investigations regarding the presence of cadv in foxes have been carried out using common serological assays which are unable to differentiate between cadv type 1 and cadv type 2. to assess the presence of viral infection in italian red foxes (vulpes vulpes), thirty-two subjects shot during the regular hunting season in the province of pisa (tuscany, it ... | 2013 | 23201241 |
| sequence-independent vidisca-454 technique to discover new viruses in canine livers. | in many mammals, viruses cause hepatitis. despite many efforts a specific virus responsible for canine idiopathic hepatitis has not been identified. the discovery of a viral etiology in canine hepatitis will promote the development of specific drugs and vaccines for the treatment of idiopathic hepatitis in dogs. the objective of this study was the application of the sequence-independent virus discovery cdna-amplified fragment length polymorphism (vidisca) technique combined with high through-put ... | 2012 | 22664180 |
| surveillance for antibodies against six canine viruses in wild raccoons (procyon lotor) in japan. | raccoons (procyon lotor) are found worldwide. they are frequently seen in crowded inner cities as well as in forests or wooded areas, often living in proximity to humans and their pets. we examined sera from 100 wild raccoons in japan for antibodies to six canine viruses with veterinary significance to assess their potential as reservoirs. we also aimed to understand the distribution of potentially infected wildlife. we found that 7% of samples were seropositive for canine distemper virus (cdv), ... | 2017 | 28715293 |
| novel adenoviruses detected in british mustelids, including a unique aviadenovirus in the tissues of pine martens (martes martes). | several adenoviruses are known to cause severe disease in veterinary species. recent evidence suggests that canine adenovirus type 1 (cav-1) persists in the tissues of healthy red foxes (vulpes vulpes), which may be a source of infection for susceptible species. it was hypothesized that mustelids native to the uk, including pine martens (martes martes) and eurasian otters (lutra lutra), may also be persistently infected with adenoviruses. based on high-throughput sequencing and additional sanger ... | 2017 | 28749327 |
| novel sequence variants of viral hexon and fibre genes in two dogs with canine adenovirus type 1-associated disease. | there is little information on sequence variation of canine adenovirus type 1 (cadv-1), the aetiological agent of infectious canine hepatitis (ich). this study reports hexon and fibre gene sequence variants of cadv-1 in a dog with systemic ich and a dog with the ocular form of the disease ('blue eye') in northern italy in 2013. one of the sequence variants matched a cadv-1 fox sequence previously detected in italy. | 2017 | 28671077 |
| detection of canine adenovirus 1 in red foxes ( vulpes vulpes) and raccoons ( procyon lotor) in germany with a taqman real-time pcr assay. | we developed a real-time (rt)pcr assay based on taqman probe technology for the specific detection of canine adenovirus 1 (cadv-1). the assay is able to detect three 50% tissue culture infectious dose/ml in cadv-1-containing cell culture supernatant. viral genomes were not amplified of canine adenovirus 2 or of several bovine, porcine, and avian adenoviruses. in silico analysis provided no indication of amplification of other heterologous genomes. the sensitivity of the real-time assay exceeded ... | 2017 | 28581363 |
| genome sequence of canine adenovirus type 1 isolated from a wolf (canis lupus) in southern italy. | canine adenovirus type 1 (cadv-1), a dna virus of the family adenoviridae, causes infectious canine hepatitis, a highly contagious disease primarily affecting canids. in this report, we describe the isolation and whole-genome sequence of a cadv-1 isolate from the liver of a free-ranging wolf (canis lupus). | 2017 | 28428308 |
| a novel recombinant canine adenovirus type 1 detected from acute lethal cases of infectious canine hepatitis. | in this study, canine adenoviruses (cadvs) from two acute fatal cases of infectious canine hepatitis (ich) were analyzed using molecular detection and sequencing of the pviii, e3, and fiber protein genes. pathological findings in affected dogs were typical for cadv-1 associated disease, characterized by severe centrilobular to panlobular necrohemorrhagic hepatitis and the development of disseminated intravascular coagulation in the terminal stages of disease. comparison of partial genome sequenc ... | 2017 | 28426340 |
| infectious canine hepatitis in a brown bear ( ursus arctos horribilis) from alaska. | we diagnosed infectious canine hepatitis in a free-ranging brown bear ( ursus arctos horribilis) cub from alaska, us, found dead in october 2015. intranuclear inclusion bodies were present in hepatocytes, and immunohistochemistry showed reactivity to adenoviral antigens. sequencing of the hexon protein of adenovirus showed 100% identity to canine adenovirus 1. | 2018 | 29498898 |
| exposure of alaska brown bears (ursus arctos) to bacterial, viral, and parasitic agents varies spatiotemporally and may be influenced by age. | we collected blood and serum from 155 brown bears (ursus arctos) inhabiting five locations in alaska, us during 2013-16 and tested samples for evidence of prior exposure to a suite of bacterial, viral, and parasitic agents. antibody seroprevalence among alaska brown bears was estimated to be 15% for brucella spp., 10% for francisella tularensis, 7% for leptospira spp., 18% for canine adenovirus type 1 (cav-1), 5% for canine distemper virus (cdv), 5% for canine parvovirus, 5% for influenza a viru ... | 2019 | 30557123 |