| in vitro studies on target cells of oncogenic adenoviruses in hamster brain. iii. in vitro transformation of brain cells of hamsters at various ages by bovine adenovirus type 3. | in vitro transformation of brain cells of hamsters at various ages was examined after the addition of bovine adenovirus type 3 to determine the type and origin of the target cells. cellular transformations occurred only in cultures of fetus and newborn animals and at low incidences. nine cell lines were obtained. virus specific tumor antigens were demonstrated in the transformed cells. the present investigation suggested that bovine adenovirus type 3 might transform mesenchymal cells (me cell) a ... | 1975 | 132085 |
| local and systemic response after simultaneous intranasal inoculation of temperature-sensitive mutants of parainfluenza 3, ibr and bovine adenovirus 3. | triple seronegative calves were exposed by the nasal route to three (ts) mutants of bovine respiratory viruses (pi3, ibr, adeno3). after a single exposure, they responded with significant levels of serum antibodies to the three viruses. nasal antibodies were demonstrated for pi3 and adenovirus antigens. the failure to demonstrate nasal antibodies to ibr may be due to lack of sensitivity of the procedure used. when reexposed six weeks later, calves had sharp increases in levels of serum antibodie ... | 1975 | 165126 |
| a bovine adenovirus type 3: isolation, characterization, and experimental infection in calves. | a virus was isolated from a group of feedlot cattle with acute respiratory disease that was characterized by physicochemical methods as an adenovirus and identified serologically as a bovine adenovirus type 3. intratracheal inoculation of three 4 month old colostrum deprived calves resulted in pyrexia, hyperpnea, dyspnea, anorexia and in one animal a mild diarrhea. | 1975 | 167684 |
| [ultrastructure of experimental virus-induced hemangiopericytomas]. | electron-microscopy studies of malignant low-differentiated hemangiopericytomas, induced by adenovirus of monkeys sa7(c8) and bovine adenovirus type 3 were, carried out. at earlier stages of the development of these tumours in tumourous cells located around capillaries there were observed the ultrastructural characteristics typical of pericytes and cells of human hemangiopericytis: the presence of the basal layer and fibrills as thick as 40 a. as differentiation proceeded, tumorous cells acquire ... | 1975 | 170893 |
| immunity studies in calves vaccinated with a multivalent live respiratory vaccine composed of i.b.r., parainfluenza 3 and bovine adenovirus type 3. | the persistence of systemic and local antibodies was studied after two intranasal administrations of the vaccine, six weeks apart. systemic antibodies to i.b.r. and adenovirus 3 evoked by the vaccine were still present 21 weeks following the second dose of the vaccine. inconslusive results were obtained regarding the persistence of systemic and local pi-3 antibodies because of an intercurrent natureal pi-3 infection occurring during the observation period. local antibodies to adenovirus type 3 w ... | 1976 | 182597 |
| temperature-dependent induction of cellular dna synthesis by bovine adenovirus type 3 in contact inhibited clonal c3h2k cells. | | 1976 | 189122 |
| investigation of causative agents of bovine respiratory tract disease in a beef cow-calf herd with an early weaning program. | serum samples were collected from early weaned fall calves shortly after the onset of respiratory tract disease. antibody titers to infectious bovine rhinotracheitis (ibr) virus, parainfluenza type 3 (pi-3) virus, bovine viral diarrhea (bvd) virus, bovine adenovirus type 3 (bav-3), and bovine respiratory syncytial virus (brsv) were determined on paired (acute and convalescent) serums. seroconversion rate (a fourfold or greater rise in antibody titer) for ibr virus was 4.3%, pi-3 virus--16.3%, bv ... | 1977 | 201196 |
| isolation and identification of a bovine adenovirus type 3 with an adenovirus-associated virus. | a bovine adenovirus with agglutinating activity was isolated from feedlot calves and classified as serotype 3. the agglutinating activity was shown to be the property of an adenovirus-associated virus (aav). the aav was isolated from the bovine adenovirus by isopycnic centrifugation in cscl; the aav had a density of 1.4 g/cm2. this aav is serologically related to bovine aav-tr-15, but is distinct from bovine parvovirus-1 and primate aav types 1 to 4, using counterimmunoelectrophoresis and hemagg ... | 1978 | 219733 |
| the indirect fluorescent antibody technique as a method for detecting antibodies in aborted fetuses. | in this investigation the indirect fluorescent antibody technique was used to titrate antibodies in bovine sera to parainfluenza 3, infectious bovine rhinotracheitis virus and bovine viral diarrhea virus. these results were compared to those determined on the same samples by hemagglutination inhibition for parainfluenza 3 virus and serum neutralization for bovine virus diarrhea and infectious bovine rhinotracheitis virus. the results of the serological methods agreed closely. the indirect fluore ... | 1979 | 226243 |
| aerosol stability of bovine adenovirus type 3. | the wbr-1 strain of bovine adenovirus type 3 was suspended in eagle's medium or bovine nasal secretion and atomized into a rotating drum at temperatures of 6 degrees c or 32 degrees c and relative humidities of 30% or 90%. impinger samples of the aerosols were collected seven minutes, one, two and three hours postgeneration, and titrated for infectivity in embryonic bovine kidney cell cultures. under certain conditions of temperature and relative humidity, the virus was more stable in aerosols o ... | 1979 | 226247 |
| a field trial with a multicomponent inactivated respiratory viral vaccine. | an inactivated virus vaccine containing strains of parainfluenza type 3 (pi3), bovine adenovirus type 3, reovirus type 1, bovine virus diarrhoea (bvd) and infectious bovine rhinotracheitis (ibr) viruses was tested in a group of 58 calves reared in a semi-intensive management system. following vaccination, 1/30, 14/30 and 17/30, showed significant rises in antibody titre to reovirus type 1, adenovirus type 3 and ibr respectively. none of the animals showed significant serological response to pi 3 ... | 1979 | 232326 |
| the relationship between age-dependent immunological competence of host and tumor growth in the hamster-bovine adenovirus type 3 system. | the relationship of aging to carcinogenesis was immunologically examined in the hamster-bovine adenovirus type 3 system. the age of animals at the time of virus inoculation influenced the tumor growth and latency period, but not the tumor incidence. the immunological competence of hamsters to sheep red blood cells became matured around 4 weeks after birth and was not affected by the infection of bovine adenovirus type 3 (bav-3). the strength of transplant immunity was dependent on the age of ani ... | 1977 | 333864 |
| tumor angiogenesis activity in clonal cells transformed by bovine adenovirus type 3. | four different sets of clonal cells transformed by bovine adenovirus type 3 and its oncogenic dna fragments, and their clonal normal counterparts, were tested for tumor angiogenesis activity. the activity was assayed by measuring the host-mediated vascular response of a chorioallantoic membrane to the cell suspension separated from the vascular bed by a millipore filter. angiogenesis activity due to inflammation reaction was prevented by using corticosteroids. all of the transformed cells tested ... | 1979 | 421213 |
| biochemical studies on bovine adenovirus type 3. iii. cleavage maps of viral dna by restriction endoncleases ecori, bamhi, and hindiii. | cleavage of bovine adenovirus type 3 (bav3) dna by restriction endonucleases ecori, bamhi, and hindiii yielded 7 (a to g), 5 (a to e), and 12 (a to l) fragments, respectively. the order of these fragments has been determined to be gdacbfe for ecori fragments, aebdc for bamhi fragments, and jebkacdhfgil for hindiii fragments, and cleavage sites of these enzymes have been mapped on the genome of bav3. bav3 preparation contains incomplete virus whose genome has a deletion of about 13% of complete v ... | 1978 | 568182 |
| biochemical studies on bovine adenovirus type 3. iv. transformation by viral dna and dna fragments. | by the calcium technique, intact dna of bovine adenovirus type 3 (bav3) was found to transform a31 cells, a clone of balb/3t3. transforming activity was resistant to rnase and pronase but sensitive to dnase. the efficiency of transformation was approximately 5 to 10 foci per mug of dna. attempts were also made to test for transforming activity of bav3 dna fragments prepared with restriction endonucleases ecori and hindiii. the activity was found to associate exclusively with the ecori d fragment ... | 1978 | 702648 |
| tumor-specific transplantation antigen(s) of bovine adenoviruses. | protection against bovine adenovirus type 3-induced primary or transplantable tumors was studied in hamsters immunized with bovine adenoviruses, human adenovirus type 12 (a-12), simian adenovirus type 7 (sa7), or chicken-embryo-lethal-orphan (celo) virus. newborn hamsters inoculated with 2.3 times 10-5 plaqueforming units of bovine adenovirus type 3 were given injections of bovine serotypes 1, 2, or 3 during the latent period of tumor development. no hamsters immunized with type 3 and only 47% o ... | 1975 | 803562 |
| changes in cell surface structure by viral transformation studied by binding of lectins differing in sugar specificity. | changes in cell surface structure by viral transformation were studied by examining changes in the binding of various lectins differing in carbohydrate specificities. binding of lectins was assayed directly using cells grown in coverslips. the following 125i-lectins were used: concanavalin-a (specific for glucose and mannose), wheat germ agglutinin (specific for n-acetylglucosamine), castor bean agglutinin (specific for galactose), wistaria floribunda agglutinin (specific for n-acetylgalactosami ... | 1975 | 815123 |
| in vitro transformation of rodent cells by simian adenovirus 7 and bovine adenovirus type 3 (strain wbr-1). | hamster, rat, and mouse embryo cells were quantitatively transformed by simian adenovirus 7 with maximum efficiencies of 2.1 x 10(5), and 2.5 x 10(5) plaque-forming unit/focus-forming unit, respectively. bovine adenovirus type 3 did not transform rat or mouse embryo cells, but transformed hamster embryo cells with a maximum efficiency of 3.4 x 10(4) plaque-forming unit/focus-forming unit. all of the transformed cells were tumorigenic in appropriate hosts and possessed viral-specific t-antigen. | 1976 | 999075 |
| oxime derivatives of erythromycin: inhibitors of rous sarcoma virus reverse transcriptase activity and focus formation. | bovine adenovirus type 3 (bav-3), strain wbr-1, quantitatively transforms hamster embryo carcass cells with a maximum efficiency of 4-7 x 10-4 p.f.u./focus-forming units (f.f.u). this frequency is signigicantly inhibited by the presence of 1-8 mm of ca++. the transformed cells are highly tumourigenic in hamsters and possess t- and tumour-specific transplantation antigens. bav-3 is one of the most oncogenic of the adenoviruses and an ideal model virus for the study of transformation. | 1975 | 1091717 |
| [biological, morphological and immunologic characteristics of cells transformed in vitro by bovine adenovirus type 3]. | studies of cell lines obtained as a result of in vitro transformation by bovine adenovirus type 3 at low multiplicities of infection: bhk-21/13-780 and bhk-21/13-782 (8 x 10(-5) tcd50/cell) and hamster embryo tissue het-1 and het-2 (2 x 10(-3) tcd50/cell) showed them to possess some oncologic characteristics typical of spontaneous neoplasia such as low degree of differentiation, high rate of cell growth, reduced adhesiveness, high tumorigenicity. in hamster embryo tissue cells (het) transformed ... | 1975 | 1162948 |
| biochemical studies on bovine adenovirus type 3. i. purification and properties. | bovine adenovirus type 3 (bav3) was purified and its properties were studied. on productive infection of ckt1 cells (a cell line derived from calf kidney) with bav3, it was observed that viral dna synthesis was initiated after about 24 h and its rate was maximal after about 40 h. maturation of the virus occurred several hours after this. purified bav3 was separated into four discrete bands by cscl density gradient centrifugation (complete, incomplete, empty, and degraded viruses). the complete b ... | 1975 | 1171993 |
| biochemical studies on bovine adenovirus type 3. ii. incomplete virus. | incomplete virus of oncogenic bovine adenovirus type 3 (bav3) was highly purified and its biological activity was studied. the production of incomplete virus was found to increase with a high multiplicity of infection and with a large amount of arginine in the growth medium. on infection of contact-inhibited mouse cells, incomplete virus induced cellular dna synthesis and focus formation. moreover, this virus was oncogenic to newborn hamsters. on infection of calf kidney cells, a permissive cell ... | 1975 | 1171994 |
| studies of virus-specific antigenicity of tumour cells transformed by bovine adenovirus type 3. | the present paper deals with the study of immunologic relationship between tumours induced by bovine adenovirus type 3 and the organism of the tumour-bearing hamster. the experiments showed that primarily induced tumours were not virus-specifically antigenic as a result of non-expression of weak tsta presumably due to the presence of more potent antigens of cellular origin. it was only after repeated passages of tumour cells in vivo or in vitro, as a result of tumour progression, that the loss o ... | 1975 | 1200779 |
| molecular cloning and restriction enzyme analysis of bovine adenovirus type 3. | bovine adenovirus type 3 (bav3) is a dna virus that causes respiratory and gastrointestinal disorders in cattle. the viral genome consists of a linear double-stranded dna molecule (35,000 base pairs) with inverted terminal repeats at each of its 5' molecular ends. we have subcloned 10 hindiii fragments spanning 4.9-96.0%, 5 ecori fragments spanning 3.4-89.5% and 2 xbai fragments spanning 35.7-82.9% of the bav3 (strain wbr-1) genome into the bacterial cloning vector puc19. the subcloning of the v ... | 1992 | 1302248 |
| sequence analysis of bovine adenovirus type 3 early region 3 and fibre protein genes. | the dna sequences of the early region 3 (e3) and fibre protein genes of bovine adenovirus type 3 (bad3) have been determined and the amino acid sequences predicted to be encoded by their open reading frames (orfs) compared to those of the fibre and e3 proteins from other ads. one of the bad3-e3 proteins contains a region homologous to the 14.7k e3 protein of human ad5 (had5). the putative bad3 fibre protein contains a number of regions homologous to the had2 fibre protein sequence, but is predic ... | 1992 | 1469367 |
| evaluation of a subunit vaccine for bovine adenovirus type 3. | the hexon subunit of bovine adenovirus serotype 3 (bav-3) was purified by use of anion-exchange chromatography. a vaccine composed of the purified hexon in immune-stimulating complexes was administered and induced high titer of virus-neutralizing antibody in rabbits and calves. | 1992 | 1575381 |
| sero-prevalences of selected cattle diseases in the kafue flats of zambia. | sera from five traditionally managed herds grazing in the kafue flats were tested for antibodies to bovine viral diarrhoea-mucosal disease (bvd-md), parainfluenza 3 (pi3), infectious bovine rhinotracheitis-infectious pustular vulvovaginitis (ibr-ipv), bovine adenovirus 3 (bav3) and bluetongue (bt). the sero-prevalences of the first four diseases were respectively 76.2, 94.4, 42.1 and 87.4%. five samples (2.3%) gave doubtful reactions for bt. prevalences of 28.5% for brucellosis, 14% for rift val ... | 1991 | 1646515 |
| first isolation of bovine adenovirus type-3 in turkey (short communication). | | 1991 | 1653696 |
| nucleotide and deduced amino acid sequence of the bovine adenovirus type 3 proteinase. | | 1990 | 2216745 |
| [antigenic determinants of the hexon of bovine adenovirus type 3]. | radioimmunoprecipitation of cattle adenovirus type 3 revealed in its hexon a determinant of narrow specificity (species-specific or subgenus-specific), intersubgenus-specific (common for cattle adenovirus subgroups 1 and 2), genus-specific (common for mammalian adenoviruses) and intergeneric (common for mammalian and avian adenoviruses) antigenic determinants. | 1986 | 2432731 |
| detection of bovine viral diarrhea virus by spot hybridization with probes prepared from cloned cdna sequences. | a cytopathic strain of bovine viral diarrhea virus (bvdv) was purified from infected cell culture fluids by isopycnic density-gradient centrifugation. genomic rna was extracted and tailed with adenine residues at the 3' end with poly-a polymerase. double-stranded complementary dna (cdna) was synthesized, using the poly-a-tailed rna as a template and oligo-dt as a primer, and then cloned into the puc9 plasmid. virus-specific cdna sequences, varying in length from 0.5 to 2.5 kilobases (kb), were o ... | 1989 | 2562207 |
| [the mutagenic action of the dna fragments of bovine adenovirus type 3 (bav-3) containing early e1 (oncogene) and e4 regions]. | a study of mutagenic action of dna fragments of bovine adenovirus type 3 (bav-3), containing early e1 (oncogenic) and e4 regions has been carried out. viral dna was digested with xba 1 restriction endonuclease. the mutagenic effect revealed itself by induction of resistance to 6-mercaptopurine in chinese hamster cells. the data obtained suggest that the early regions e1 and e4 can cause mutagenic effect in mammalian cells. | 1989 | 2760327 |
| bovine adenovirus type-3 infection in feedlot calves. | the frequency of bovine adenovirus type-3 (ba3) infection in 84 of 165 light feeder calves and the possible role of ba3 in inducing febrile disease and affecting weight gain performance were studied for 56 days after the calves entered a feedlot. samples of blood for serotesting were obtained periodically, and samples for virus isolation were collected on days 28 and 56. at the time the calves entered the feedlot, 17.8% (15 of 84) had serum virus-neutralizing antibodies to ba3. during 56 days, 5 ... | 1988 | 2833138 |
| vaccination of dairy calves with bovine adenovirus type 3. | a field study was undertaken to determine the efficacy of vaccinating dairy calves with a killed bovine adenovirus type 3 (ba3) vaccine in a herd where pneumonia associated with ba3 infection had been a severe problem. calves were first vaccinated when they were less than one week old; a second dose was given 10-14 days later. efficacy of the vaccine was evaluated following natural exposure to the virus by comparing prevalence of pneumonia in control (n = 21) and vaccinated (n = 21) calves. sero ... | 1987 | 2833376 |
| prevalence of antibodies to seven viruses in a flock of ewes in minnesota. | blood samples were collected from a flock of healthy ewes at a university of minnesota research station. sera from these blood samples were tested for antibodies against 7 viruses, using 3 tests (eg, virus-neutralization test for bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, bovine adenovirus type 3, and bovine respiratory syncytial virus; hemagglutination inhibition test for parainfluenza virus type 3; and agar-gel immunodiffusion test for lentivirus of ovine progressive ... | 1988 | 2837112 |
| characteristics of in vitro transformed cells essential for their in vivo survival, selection and metastasizing activity. | two questions were investigated: (1) the dynamics of in vivo selection of tumor cells possessing metastasizing activity (ma) at the time preceding the appearance of visible lung metastases, and (2) the possible connection of in vivo selection of metastatic tumor cells with their susceptibility to h2o2 produced by the cytotoxic oxidative burst of activated neutrophils, monocytes and macrophages. spontaneously in vitro transformed syrian hamster embryo cells (strain sthe), never selected in vivo a ... | 1986 | 3005179 |
| nitrogen metabolism of calves inoculated with bovine adenovirus-3 or with infectious bovine rhinotracheitis virus. | beef calves were inoculated with bovine adenovirus-3 or infectious bovine rhinotracheitis virus. after inoculation, plasma fibrinogen increased, serum phosphorus decreased, and nitrogen and phosphorus digestibility decreased compared with preinoculation values. urinary n excretion increased when calves developed rectal temperatures greater than 39.7 c. results indicated that clinical infection of calves with infectious bovine rhinotracheitis virus increases urinary n excretion and reduces n and ... | 1986 | 3013053 |
| [comparison of methods of detection of bovine adenovirus serotype 3 in infected culture of calf kidney cells (mdbk)]. | the comparative study of the dynamics of the main antigen (hexon) and viral dna of the bovine adenovirus type 3 accumulation in the established cell line mdbk under the conditions of single- or multistep cycle of infection has been undertaken. the quantitative immunoelectrophoresis and immunoenzyme assay detected the viral antigens on the late stages of infection in the period of cellular monolayer degradation. the immunofluorescence reaction and histochemical immunoenzyme method detected the an ... | 1988 | 3068542 |
| dna sequence of the adenovirus type 41 hexon gene and predicted structure of the protein. | the gene for the major capsid protein (hexon) of human adenovirus type 41 (ad41) has been isolated and the complete dna sequence determined. comparison of the predicted amino acid sequence with hexons from human ad2 and ad5 and bovine adenovirus type 3 reveals regions of high homology at the n and c termini separated by a central region of low homology. fitting of the ad41 hexon sequence to the known three-dimensional structure of the ad2 hexon demonstrates that both hexons have a common archite ... | 1988 | 3411299 |
| the action of the tumour promoter, tpa, on mutagenesis induced by different agents (uv light, chemical and viral mutagens). | the present paper deals with effects of 12-o-tetradecanoylphorbol-13-acetate (tpa) on the frequency of induced mutations to 6-mercaptopurine (6mp) and ouabain resistance in chinese hamster and mouse cells. uv light, bovine adenovirus 3(bav-3) and 5-bromodeoxyuridine (brdu) were used as mutagens. tpa was shown to raise the frequency of gene mutations induced by uv light and bav-3 but it did not enhance the mutagenic effect of brdu. we also examined the ability of bav-3 and brdu to induce tumours ... | 1987 | 3614245 |
| study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves. | bovine respiratory syncytial virus (brsv) was the viral agent most commonly identified in 14 epizootics of pneumonia in dairy calves. a microtiter serum-virus neutralization test proved to be the best means of identifying involvement of brsv; seroconversion (fourfold or greater rise in titer) was demonstrated in 10 of the 14 epizootics. only limited involvement of bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and bovine adenovirus type 3 was recogni ... | 1986 | 3733504 |
| mdbk nuclear factor-binding site of various serotypes of adenovirus dna. | a fraction with the ability to bind the terminal fragment of equine adenovirus (ead) dna was prepared from mdbk cell nuclei. the fraction (mdbk nuclear factor) bound to the terminal fragment of all human and animal adenovirus dnas examined except avian adenovirus eds-76. however, the terminal fragments of two animal adenoviruses, ead and bovine adenovirus type 3 (bad3), showed higher affinity for the nuclear factor than the others. the mdbk nuclear factor-binding site determined by footprinting ... | 1986 | 3796315 |
| seroepizootiologic study of bovine respiratory syncytial virus in a beef herd. | a seroepizootiologic study of bovine respiratory syncytial virus in a beef herd was conducted from february 1983 through march 1984. during the study period, 3 separate respiratory tract disease epizootics were recognized in calves after they had been split into 3 groups. bovine respiratory syncytial virus infection was diagnosed in each epizootic on the basis of serologic evidence, postmortem findings, and immunofluorescent examination of lung tissue. additionally, there was serologic evidence ... | 1986 | 3954199 |
| [tpa enhancement of the mutagenic and transforming action of bovine adenovirus type 3 on cultured mouse cells]. | the cultured 10ti/2c3h mouse cells were infected with bovine adenovirus 3(bav-3) and treated by tpa. this combined treatment induced ouabain-resistant mutants 19 to 26 times more often than action of only bav-3. bav-3- and tpa-treated cells were implanted to syngenic mice. the tumours appeared 10 times more often than in the mice after implantation of only adenovirus treated cells. | 1985 | 3979337 |
| choroid plexus papilloma and giant cell glioblastoma induced in hamsters with bovine adenovirus type 3. | newborn hamsters were inoculated with 0.02 ml of bovine adenovirus type 3 (bav 3) having a titer of 10(4.5)tcid50/0.1 ml. of 82 hamsters observed over 40 days after viral inoculation, 15 developed choroid plexus papilloma after a latency period of 41-228 days and six developed giant cell glioblastoma after a latency period of 66-214 days. multiple foci of abnormal cell growth were also observed in the meninges and choroid plexus. choroid plexus papilloma developed in the ventricles occupying the ... | 1985 | 4013674 |
| [role of the oncogene in the mutagenic activity of bovine adenovirus type 3]. | the mutagenic and carcinogenic effect of two ecori-fragments of bovine adenovirus type 3 (bav-3) dna inserted into pbr325 has been studied. the c fragment (located between 3,6 and 19,7 map units) contains the viral oncogene, the c fragment (between 44,3 and 63,7 map units) displays no transforming activity. it has been established that oncogene bav-3 statistically true increases the yield of mutants resistant to 6-mercaptopurine (6mp) in chinese hamster cells. the c fragment, pbr325 without vira ... | 1985 | 4040773 |
| [mutagens and the tumor promoter]. | we studied the effect of 12-o-tetradecanoylphorbol-13-acetate (tpa) and saccharin on the frequency of induced mutations of resistance to 6-mercaptopurine and ouabain in chinese hamster and mouse cells. uv-rays, bovine adenovirus-3 (bav-3) and 5-bromdeoxyuridine (brdu) were used as mutagens. in the case of bav-3 and brdu, we investigated, apart from the mutagenic effect, the tumor-inducing activity of these mutagens in mice, brdu proved to have no carcinogenic effect. the data about the influence ... | 1985 | 4054615 |
| pathological studies on carcinogenesis by bovine adenovirus type 3. 3. tumor induction by bovine adenovirus type 3 in c3hf-bi mouse treated with n,n'-dimethylnitrosourea. | | 1972 | 4268661 |
| effects of antithymocyte serum and thymectomy on the growth of tumors induced by bovine adenovirus type 3. | | 1973 | 4271878 |
| the pathogenesis and pathology of infection in calves with a strain of bovine adenovirus type 3. | | 1966 | 4287957 |
| oncogenicity of bovine adenovirus type 3 in hamsters. | | 1966 | 4290895 |
| temperature-dependent cellular regulation in induction of cellular deoxyribonucleic acid synthesis by bovine adenovirus type 3. | | 1973 | 4541886 |
| nonproductive infection and induction of cellular deoxyribonucleic acid synthesis by bovine adenovirus type 3 in a contact-inhibited mouse cell line. | bovine adenovirus type 3 (bav-3), which has been reported to produce tumors in newborn hamsters, induced cellular deoxyribonucleic acid (dna) synthesis in a contact-inhibited mouse kidney cell line (c3h2k). in this system, the virus did not multiply, whereas virus-specific tumor antigen (t antigen) was detected in nearly all cells. replication of viral dna could not be detected by dna-dna hybridization on membrane filters. the cellular dna synthesis induced by bav-3 did occur in the absence of a ... | 1972 | 4552552 |
| growth characteristics of tumors induced by bovine adenovirus type-3 in hamsters of various ages. | | 1972 | 4566614 |
| clonal analysis of tumorigenesis of bovine adenovirus type 3 and isolation of a nontumorigenic variant. | | 1970 | 4986497 |
| neoplastic transformation of hamster cells in vitro by bovine adenovirus type-3. | | 1972 | 5081620 |
| [some biological properties and morphological characteristics of transplantable hamster tumor, induced by bovine adenovirus type 3]. | | 1971 | 5131042 |
| morphology of tumors induced in hamster soft tissues by bovine adenovirus type 3. | | 1971 | 5137314 |
| growth of bovine adenovirus type 3 in cells cloned from a cell line of calf kidney. | | 1971 | 5169311 |
| studies on the oncogenicity of bovine adenovirus type 3. | | 1968 | 5724522 |
| bovine adenovirus type 3: detection of specific tumor and t antigens. | | 1967 | 6022697 |
| serological studies of parainfluenza type 3 virus, bovine adenovirus type 3 and bovine respiratory syncytial virus infection in beef calves. | six serum samples were taken at monthly intervals from birth to weaning from each of 41 newborn calves in the autumn and spring calf crops of a beef cow--calf herd. the serum hemagglutination-inhibition (hi) antibody titres to parainfluenza type 3 virus (piv-3), virus-neutralization (vn) antibody titres to bovine adenovirus type 3 (bav-3) and bovine respiratory syncytial virus (brsv) were determined using microtitration techniques. there was serological evidence of a significantly higher inciden ... | 1984 | 6095517 |
| a seroepidemiologic survey of three pronghorn (antilocapra americana) populations in southeastern idaho, 1975-1977. | sera from 104 adult and 42 fawn pronghorn antelope (antilocapra americana) from southeastern idaho were tested against selected livestock pathogens. the numbers positive/numbers tested (% positive) were as follows: bovine virus diarrhea - adults 2/102 (2), fawns 0/41 (0);; infectious bovine rhinotracheitis - adults 27/101 (27), fawns 9/42 (22); parainfluenza 3 - adults 79/104 (76), fawns 22/42 (52); bovine adenovirus 7 - adults 42/103 (41), fawns 20/48 (48); bovine adenovirus 3 - adults 11/32 (3 ... | 1980 | 6246285 |
| alimentary tract manifestations of bovine adenovirus infections. | two calves and a feedlot steer with systemic bovine adenovirus infection had hemorrhagic and necrotizing lesions confined to the digestive tract. in one calf there was severe, multifocal, necrotizing, hemorrhagic rumenitis, omasitis, abomasitis, enteritis and colitis. small intestinal lesions were predominantly in peyer's patches. multifocal necrotizing abomasitis was the principal change in the other calf. large amphophilic intranuclear inclusions typical of bovine adenovirus were common in swo ... | 1981 | 6269717 |
| adenovirus-related rna sequences in human neurogenic tumours. | thirty two human tumours, mainly neurogenic, have been investigated for the presence of adenovirus-related rna sequences. 3h-labelled tumour virus dna probes derived from human adenoviruses types 2 and 12, bovine adenovirus type 3, and avian adenovirus celo were hybridized in-situ on tumour kryostat sections under conditions that detect complementary rna. tumour virus-related rna was detected in 62% of all tumours tested, but was not detectable in normal human brain tissues. expression of tumour ... | 1982 | 6278813 |
| a study of rat embryo cells transformed in vitro by the bovine adenovirus type 3 (bav-3) dna before and after a passage in the host. | | 1982 | 6294984 |
| a study of viral genomes in cells transformed by the nononcogenic human adenovirus type 5 and highly oncogenic bovine adenovirus type 3. | | 1984 | 6488880 |
| [malignant transformation of mouse cultured cells using bovine adenovirus type 3 and modification of this process by mnng]. | it is shown that tumour transformation of cultured mouse cells 10t1/2c3h depends on the time after the infection with virus bav-3. the maximal number of tumours was observed 3 weeks after infection, then the frequency of tumours was reduced. mnng modified virus-induced tumour cell transformation differently depending on the time between cell infection and treatment with the chemical agent. | 1984 | 6510341 |
| [mutagenic action of the dna of the highly oncogenic bovine adenovirus type 3 (bav-3)]. | the induction of mutations to the 6-mercaptopurine resistance has been studied in chinese hamster cells after transfection with highly oncogenic bovine adenovirus type 3 dna (bav-3). a statistically significant increase in the yield of resistant mutants is induced by viral dna after two days expression time. the value of induction varies from 56,12 x 10(-5) to 70,02 x 10(-5). the comparison of the mutagenic activity of dna and the intact viral particles has revealed that the number of dna molecu ... | 1983 | 6683105 |
| sequence homology between bovine and human adenoviruses. | cross-hybridization has been detected between corresponding regions of the genomes of bovine adenovirus type 3 and human adenovirus type 2. the most conserved region on the viral genomes encodes the hexon polypeptide. the nucleotide sequence of this region in bovine adenovirus type 3 has been determined. comparison of the predicted amino acid sequences of the bovine adenovirus type 3 and human adenovirus type 2 hexon polypeptides reveals three regions of nonhomology. | 1984 | 6694264 |
| [comparative study of sialyltransferases in cultured cells of the rat embryo, transformed by dna bovine adenovirus type 3]. | the specific activities of membrane-bound sialyl transferases from six cultures of cells transformed by bovine adenovirus dna type 3 (ba-3) before and after passage through host animal organism were compared. the sialyl transferase activity was increased with respect to the high molecular weight acceptor, asialylofetuin in the majority of transformed and tumour cultures. identification of sialylation products by paper chromatography revealed that all cell cultures contain sialyltransferases synt ... | 1984 | 6713023 |
| bovine respiratory syncytial virus in quebec: antibody prevalence and disease outbreak. | the prevalence of antibody to bovine respiratory syncytial virus in quebec and the role of the virus in a respiratory disease outbreak was investigated. the indirect immunofluorescent, neutralization and haemagglutination inhibition techniques were used to carry out this study. of the 1,444 adult animals examined 519 (35.9%) had antibody to bovine respiratory syncytial virus. these positive reactors were found in each agricultural region of quebec. the highest (53.0%) and the lowest (21.8%) prev ... | 1980 | 7000320 |
| [induction of chromosome aberrations in chinese hamster cells by oncogenic bovine adenovirus type 3]. | | 1980 | 7190444 |
| [isolation and biological properties of rat embryo cells transformed by the dna of bovine adenovirus type 3 (ba-3)]. | cloned cultures of rat embryo cells transformed by ba-3 dna and those transformed by the virus in hamsters were obtained. it was found that these cultures contain t-antigen and do not differ in oncogenicity for hosts. the adenovirus type 5 dna transformed cells of the same primary culture of rat embryo fibroblasts produced no tumors in syngeneic newborn rats. the role of the viral genome in oncogenicity of the cells transformed by it is discussed. | 1981 | 7456443 |
| pathogenesis and immunogenicity of bovine adenovirus type 3 in cotton rats (sigmodon hispidus). | intranasal inoculation of cotton rats (sigmodon hispidus) with 10(8) pfu of bovine adenovirus type 3 (bad3) resulted in limited virus replication in the lung and trachea. histopathological changes in the lungs were characterized by necrosis and hyperplasia of bronchiolar epithelium, eosinophilic intranuclear inclusions, pneumocyte type ii hyperplasia in the alveoli, and mild peribronchiolar and perivascular lymphocytic infiltration. immunohistochemically, viral antigens were observed more freque ... | 1995 | 7483256 |
| identification and sequence analysis of the e1 genomic region of a porcine adenovirus. | the complete nucleotide sequence of the putative early transcriptional region 1 (e1) of the genome of nadc-1, a porcine adenovirus, was determined. the e1 region of nadc-1 was found to be 3658 bp and located between 0 and 11.2 map units. twelve potential open reading frames (orfs) and five polyadenylation signals were identified in the r strand. the nucleotide sequence and each predicted amino acid sequence were compared to sequences available on a number of databases by a blast search and compa ... | 1995 | 7653103 |
| development of a bovine adenovirus type 3-based expression vector. | we constructed a non-defective bovine adenovirus type 3 recombinant (bad3-luc) containing the firefly luciferase gene inserted in the early region 3 (e3) of the bad3 genome. deletion of a 696 bp xhoi-ncoi e3 segment and insertion of the luciferase gene in e3 was confirmed by southern blot analyses. luciferase was expressed in madin-darby bovine kidney cells infected with bad3-luc as measured by enzymic assays and western blotting. analyses of luciferase expression in the presence or absence of 1 ... | 1995 | 7844546 |
| the fibre of bovine adenovirus type 3 is very long but bent. | the sequence of the fibre of bovine adenovirus type 3 (bad3) predicts an extremely long structure due to a large number of 15-residue repeats in the fibre shaft, the tail and head domains being similar in size to the human adenovirus fibres. the length of the fibre was confirmed using negative-strain electron microscopy of bad3 pentons (fibre plus penton base). the fibre was found to be bent in several discrete places and the bending sites appear to correspond with irregular repeats in the shaft ... | 1994 | 8046411 |
| sequence and sequence analysis of e1 and pix regions of the bav3 genome. | bovine adenovirus type 3 (bav3) is a dna virus that causes respiratory and gastrointestinal disorders in cattle. we have sequenced the extreme left end of bav3 genome (0-11.7 map units). partial analysis of the nucleotide sequence revealed 19 potential open reading frames (orfs) that could encode for polypeptides of 50 or more amino acids. four of these orfs show homology to known adenovirus polypeptides. the four bav3 orfs are located in approximately the same area as the ad5 e1a, e1b, and pix ... | 1993 | 8150592 |
| restriction endonuclease analysis and molecular cloning of porcine adenovirus type 3. | the 6618 strain of porcine adenovirus type 3 was cultivated in swine testis cells, and viral dna was extracted from the infected cells by a modified hirt procedure. the dna was digested by each of 13 restriction endonucleases, and the number of cleavage sites which were identified varied from 1 to 17. the size of the porcine adenovirus type 3 genome, estimated from the sizes of the restriction enzyme fragments, was approximately 35 kb. fragments representing the entire genome were cloned. physic ... | 1993 | 8150597 |
| the e1 sequence of bovine adenovirus type 3 and complementation of human adenovirus type 5 e1a function in bovine cells. | the bovine adenovirus type 3 (bav3) genome was sequenced from the left end to the hindiii site at 11%. this region comprises the entire e1 transcription unit including the open reading frames (orf) for proteins homologous to the e1a, e1b proteins and protein ix of human adenovirus type 5 (ad5). a portion of the bav3 e1a protein showed significant homology with conserved region 3 (cr3), the principal transactivation region of ad5 e1a. the bav3 e1a protein also contains a consensus sequence known ... | 1994 | 8178572 |
| sequence analysis of bovine adenovirus type 3 early region 3 and fibre protein genes. | | 1993 | 8277294 |
| pathology and immunogenicity in the cotton rat (sigmodon hispidus) model after infection with a bovine adenovirus type 3 recombinant virus expressing the firefly luciferase gene. | the histopathology of adenovirus pneumonia in cotton rats (sigmodon hispidus) due to bovine adenovirus type 3-luciferase recombinant virus (bad3-luc), which has a 0.7 kb deletion from the early region 3 (e3) replaced with the firefly luciferase gene, was compared with that produced by the parental wild-type (wt) bovine adenovirus type 3 (bad3). after intranasal inoculation of cotton rats with 3 x 10(7) p.f.u. of bad3-luc, the infectious virus titres in the lungs at various times post-infection w ... | 1996 | 8558115 |
| [seroprevalence of viral infections in llamas (lama glama) in the republic of argentina]. | this study reports the seroprevalence of bovine viral infections in llamas (lama glama) in argentina. this is the first study made in the country including 390 llamas and testing antibodies against eight viruses. samples were collected from nine farms distributed in three different provinces: buenos aires, córdoba and jujuy. the samples were tested for antibodies against eight viruses known to infect cattle: bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine adenovirus ... | 1997 | 9229724 |
| hydropathic characteristics of adenovirus hexons. | the complete nucleotide sequence and the predicted amino acid sequence of the adenovirus type 7 hexon gene were determined. the hydropathy of the hexon proteins from human adenovirus types 2, 3, 4, 5, 7, 12, 16, 40, 41, and 48, bovine adenovirus type 3, murine adenovirus type 1, and avian adenovirus types 1 and 10 was analysed. the presence of purines and pyrimidines in the second position of the codons was correlated to hydrophilicity and hydrophobicity, respectively. comparison of the hydrophi ... | 1997 | 9267445 |
| nucleotide sequence, genome organization, and transcription map of bovine adenovirus type 3. | the complete dna sequence of bovine adenovirus type 3 is reported here. the size of the genome is 34,446 bp in length with a g+c content of 54%. all the genes of the early and late regions are present in the expected locations of the genome. however, the late-region genes are organized into seven families, instead of five as they are in human adenovirus type 2. the deduced amino acid sequences of open reading frames (orfs) in the late regions and early region 2 (e2) and for iva2 show higher degr ... | 1998 | 9445040 |
| characterization of bovine adenovirus type 3 early region 2b. | we have determined the nucleotide sequence of a 6999 base pair region of bovine adenovirus-3 covering map units 9.0 to 29.17, which contained the adenovirus homologs of iva2 protein and the dna replication proteins, precursor of terminal protein and dna polymerase proteins. analysis of the sequence for cis-acting elements suggests that transcripts of dna polymerase and precursor of terminal protein are 3' co-terminal. in addition, this region also contains major late promoter sequence. the seque ... | 1998 | 9654686 |
| long-term viral serology of semi-free-living and captive ungulates. | between 1973 and 1994, blood samples were collected at whipsnade wild animal park (uk) from three ungulate species kept in enclosures, including 28 european bison (bison bonasus), 37 scimitar-horned oryx (oryx dammah), and 49 père david's deer (elaphurus davidianus), and also from 144 semi-free-living chinese water deer (hydropotes inermis). these samples were tested for the presence of antibodies against three bovine viral diarrhea virus (bvdv)-like strains, three alpha-herpesvirus strains, enz ... | 1998 | 9732031 |
| construction and characterization of e3-deleted bovine adenovirus type 3 expressing full-length and truncated form of bovine herpesvirus type 1 glycoprotein gd. | using the homologous recombination machinery of e. coli, a 1.245-kb deletion was introduced in the e3 region of bovine adenovirus 3 (bav3) genomic dna cloned in a plasmid. transfection of the restriction enzyme-excised, linear e3-deleted bav3 genomic dna into primary fetal bovine retina cells produced infectious virus (bav3. e3d), suggesting that all the e3-specific open reading frames are nonessential for virus replication in vitro. using a similar approach, we constructed replication-competent ... | 1998 | 9770436 |
| genetic organization and dna sequence of early region 4 of bovine adenovirus type 3. | we have identified and sequenced 3614 nucleotides located at the extreme right-end of the bovine adenovirus type 3 (bav3) genome from map units 89.5-100. analysis of the sequence revealed an inverted terminal repeat (itr) of 195 bp, and identified five open reading frames (orfs) designated orf1, orf2, orf3, orf4 and orf5. when compared with known e4 orfs of other adenoviruses, orfs 1, 2 and 4, which code for proteins of 143, 69 and 143 amino acids respectively, were found to be unique to bav3. o ... | 1998 | 9778793 |
| nucleotide sequence and transcription map of porcine adenovirus type 3. | the complete nucleotide sequence of porcine adenovirus type 3 was determined and a transcriptional map for the genome was constructed. the size of the genome is 34094 bp in length with an unusually high g + c content (63.7%), the highest thus far reported for any adenovirus. overall organization of the genome is similar to that for previously sequenced adenoviral dnas, but there also were distinct differences. the late regions genes are organized into six families, instead of five as they are in ... | 1998 | 9837805 |
| characterization of early region 1 and pix of bovine adenovirus-3. | the mrnas from early region 1 (e1) and pix of bovine adenovirus type 3 (bav-3) have been studied by northern blot, s1 nuclease, and cdna analysis and transcriptional maps for the regions were constructed. the transcriptional map for the e1 region of bav-3 is different from those of mouse and human adenoviruses for which transcriptional maps for the regions have been constructed. the e1a region of bav-3 is located between 0.8 and 10.5 map units and several different transcripts are produced from ... | 1999 | 9918888 |
| generation of infectious genome of bovine adenovirus type 3 by homologous recombination in bacteria. | the widely used technique of generating adenovirus vectors by homologous recombination in mammalian cells is usually not very efficient. this communication describes a simple method of generating a plasmid containing the full-length genome of an adenovirus by homologous recombination in bacteria. following transfection of a suitable mammalian cell line with the full-length adenovirus genome, infectious virus progeny could easily be generated. using this technique the generation of adenovirus rec ... | 1999 | 10092136 |
| transcription mapping and characterization of 284r and 121r proteins produced from early region 3 of bovine adenovirus type 3. | we established the transcription map of early region (e) 3 of bovine adenovirus 3 (bav-3) by northern blot, s1 nuclease protection assays, cdna sequencing, and rt-pcr analysis. five major classes of mrnas were identified, which shared the 3' ends. four classes of mrnas transcribed from the e3 promoter also shared the 5' end, while one major class of mrna transcribed from the major late promoter contained a tripartite leader sequence at the 5' end. these five transcripts have the potential to enc ... | 1999 | 10191200 |
| mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1. | to determine the potential of replication-competent (e3-deleted) bovine adenovirus-3 (bav-3) as a delivery system for vaccine antigens in calves, we evaluated the ability of recombinant bav-3 expressing different forms of of bovine herpesvirus-1 (bhv-1) glycoprotein gd to protect against bhv-1 infection in calves that had pre-existing bav-3 specific antibodies. three- to four-month-old calves, vaccinated intranasally with recombinant bav-3 expressing full-length gd (bav3.e3gd) or a truncated ver ... | 1999 | 10355773 |
| biodegradable alginate microspheres as a delivery system for naked dna. | sodium alginate is a naturally occurring polysaccharide that can easily be polymerized into a solid matrix to form microspheres. these biodegradable microspheres were used to encapsulate plasmid dna containing the bacterial beta-galactosidase (lacz) gene under the control of either the cytomegalovirus (cmv) immediate-early promoter or the rous sarcoma virus (rsv) early promoter. mice inoculated orally with microspheres containing plasmid dna expressed lacz in the intestine, spleen and liver. ino ... | 1999 | 10369574 |
| experimental inoculation of heifers with bovine adenovirus type 3. | nine 2-year-old heifers having bad3-neutralizing antibody titers between 1:120 and 1:1080 were individually exposed intranasally to an aerosol of 10(8) pfu of wild type (wt) bovine adenovirus type 3 (bad3). four animals were kept as non-inoculated controls. the heifers were examined daily for rectal temperature, weight gain/loss, nasal and ocular discharges, and other clinical signs for 10 d post-inoculation. none of the animals showed any sign of clinical disease. virus excretion was observed i ... | 1999 | 10369575 |
| transcription units of e1a, e1b and pix regions of bovine adenovirus type 3. | the major mrna species in the e1 region of the genome of bovine adenovirus type 3 (bav3) have been defined by using a combination of pcr, 5' race, northern analysis and dna sequencing. independent transcription initiation sites were identified for each of the e1a, e1b and protein ix (pix) transcription units, but all mrna species terminated at the same poly(a) addition site immediately downstream of the pix open reading frame. thus, the bav3 e1 region, which consists of the e1a and e1b genes tog ... | 1999 | 10423142 |
| transcription map and expression of bovine herpesvirus-1 glycoprotein d in early region 4 of bovine adenovirus-3. | early region 4 (e4) of bovine adenovirus type 3 (bav-3) was analyzed by northern blotting, rt-pcr analysis, cdna sequencing, and s1 nuclease protection assays. the transcriptional map of the e4 region of bav-3 has marked dissimilarities from those of mouse adenovirus-1, ovine adenovirus-287, and human adenovirus-2, for which the transcriptional maps have been constructed. the e4 region of bav-3, located between 98.6 and 89.8 mu transcribes seven distinct classes of bovine adenovirus type 3 mrna. ... | 1999 | 10441562 |
| the adenovirus that causes hemorrhagic disease of black-tailed deer is closely related to bovine adenovirus-3. | dna sequence data was obtained from an adenovirus previously shown to be the cause of a distinctive, fatal hemorrhagic disease of black-tailed deer in california. a 256 base fragment of the viral hexon gene was amplified by pcr from purified adenovirus preparations. the amplicon then was cloned and sequenced. phylogenetic relationships with other mammalian adenoviruses were also determined. although sequence analysis of this portion of the hexon gene indicates that the black-tailed deer adenovir ... | 1999 | 10470262 |