| proposals for a new classification of iridescent viruses. | the need for comparative studies of iridoviruses to elucidate the relationships between them has been well appreciated. sixteen iridoviruses, including type species from each of the four recognized genera of the iridoviridae, were compared by restriction endonuclease characterization, hybridization to the major structural protein (msp) gene of an invertebrate iridescent virus (iv) isolate at various stringencies, pcr amplification of the msp gene region and by dot-blot hybridization studies. the ... | 1994 | 8207395 |
| molecular characterization, sequence analysis, and taxonomic position of newly isolated fish iridoviruses. | within the past decade, iridoviruses have been identified as the causative agents of systemic disease in a variety of commercially and recreationally important fish. here we examine nine iridoviruses from fish, reptiles, and amphibians and demonstrate that all isolates were more similar to frog virus 3, the type species of the genus ranavirus, than to lymphocystis disease virus, the type species of the genus lymphocystivirus. comparison of viral protein synthesis profiles, restriction endonuclea ... | 1997 | 9123863 |
| the complete dna sequence of lymphocystis disease virus. | lymphocystis disease virus (lcdv) is the causative agent of lymphocystis disease, which has been reported to occur in over 100 different fish species worldwide. lcdv is a member of the family iridoviridae and the type species of the genus lymphocystivirus. the virions contain a single linear double-stranded dna molecule, which is circularly permuted, terminally redundant, and heavily methylated at cytosines in cpg sequences. the complete nucleotide sequence of lcdv-1 (flounder isolate) was deter ... | 1997 | 9143276 |
| molecular anatomy of lymphocystis disease virus. | lymphocystis disease (ld) has been reported to occur in over one hundred different species of fish worldwide. the disease is caused by lymphocystis disease virus (lcdv), a member of the iridovirus family. numerous fish species that play an important role in fishery and fish farming are highly susceptible to lcdv infection. the infected animals develop disseminated clusters of aberrant hypertrophied cells within their connective tissue, the so-called lymphocystis cells. in the cytoplasm of these ... | 1997 | 9413525 |
| the dna sequence of chilo iridescent virus between the genome coordinates 0.101 and 0.391; similarities in coding strategy between insect and vertebrate iridoviruses. | chilo iridescent virus (civ), the type species of the genus iridovirus within the family iridoviridae, is highly pathogenic for larvae of important pest insects. the virions contain a single linear double-stranded dna molecule (209 kbp) that is circularly permuted and terminally redundant. the nucleotide sequence of the viral genome between the genome coordinates 0.101 and 0.391 (60,170 bp) was determined by automated cycle sequencing. this particular region of the civ genome contains 112 open r ... | 1997 | 9482589 |
| viral dna sequences of genes encoding the atpase and the major capsid protein of tropical iridovirus isolates which are pathogenic to fishes in japan, south china sea and southeast asian countries. | tropical iridovirus infection causes severe epizootic resulting in mass mortalities and large economic losses in freshwater ornamental fishes cultured in southeast asian countries, in wild fish seedlings captured in south china sea, and in marine fishes farmed in japan, singapore, and thailand. all of tropical iridovirus-infected fishes histopathologically showed the systemic formation of inclusion body-bearing cells and necrosis of virus-infected splenocytes and hematopoietic cells. we designed ... | 2002 | 12417946 |
| cladistic analysis of iridoviruses based on protein and dna sequences. | cladograms of iridoviruses were inferred from bootstrap analysis of molecular data sets comprising all published protein and dna sequences of the major capsid protein, atpase and dna polymerase genes of members of the iridoviridae family iridovirus. all data sets yielded cladograms supporting the separation of the iridovirus, ranavirus and lymphocystivirus genera, and the cladogram based on data derived from major capsid proteins further divided both the iridovirus and ranavirus genera into two ... | 2003 | 14579177 |
| investigations on the orf 167l of lymphocystis disease virus (iridoviridae). | the predicted open reading frame 167l (orf 167l) of lymphocystis disease virus (lcdv, iridoviridae ) isolated from plaice, dab and flounder was investigated. the orf 167l corresponding genes of the three lcdv isolates were amplified, cloned and sequenced. a comparison of the lcdv strains showed that the nucleotide sequence of orf 167l and its deduced amino acid sequence were highly conserved in the genus lymphocystivirus (a homology of 80% in dab and flounder/plaice, 97% in plaice and flounder). ... | 2004 | 14739649 |
| identification of a bohle iridovirus thymidine kinase gene and demonstration of activity using vaccinia virus. | in recent years interest in the family iridoviridae has been renewed by the identification of a number of viruses, particularly from the genus ranavirus, associated with disease in a range of poikilotherms. ranaviruses have been isolated from amphibian, piscine and reptilian species. here we describe an open reading frame (orf) identified in the genome of bohle iridovirus (biv) which contains a nucleotide binding motif conserved within the thymidine kinase (tk) genes of iridoviruses from other g ... | 2005 | 15883656 |
| phylogenetic analysis of the major capsid protein gene of iridovirus isolates from cultured flounders paralichthys olivaceus in korea. | in 2003, 13 isolates of iridovirus were obtained from cultured flounders paralichthys olivaceus during epizootics in korea. the full open reading frames (orfs) encoding the major capsid protein (mcp) (1362 bp) from the 13 flounder iridoviruses (flivs) were sequenced and the deduced amino acid sequences were phylogenetically analyzed. phylogenetic analysis of the mcp revealed that all 13 flivs were the same species as rock bream iridovirus (rbiv), red sea bream iridovirus (rsiv), and infectious s ... | 2005 | 15997817 |
| a new genotype of lymphocystivirus, lcdv-rf, from lymphocystis diseased rockfish. | lymphocystis disease virus (lcdv) is the causative agent of lymphocystis disease. in this study, nucleotide sequences of the major capsid protein (mcp) gene were analyzed among lcdv isolates from japanese flounder and rockfish. a phylogenetic tree revealed three clusters for lymphocystiviruses. the first cluster included japanese flounder isolates; the second cluster consisted of rockfish isolates; and the remaining one consisted of lcdv-1. nucleotide sequence identities were > or =99.6% among j ... | 2006 | 16328139 |
| genome of invertebrate iridescent virus type 3 (mosquito iridescent virus). | iridoviruses (ivs) are classified into five genera: iridovirus and chloriridovirus, whose members infect invertebrates, and ranavirus, lymphocystivirus, and megalocytivirus, whose members infect vertebrates. until now, chloriridovirus was the only iv genus for which a representative and complete genomic sequence was not available. here, we report the genome sequence and comparative analysis of a field isolate of invertebrate iridescent virus type 3 (iiv-3), also known as mosquito iridescent viru ... | 2006 | 16912294 |
| [inverse pcr amplification of the complete major capsid protein gene of lymphocystis disease virus isolated from rachycentron canadum and the phylogenetic analysis of the virus]. | the major capsid protein of lymphocystis disease virus isolated from rachycentron canadum (lcdv-rc) was amplified and analysed. the 457bp dna core fragment was amplified with the degenerate primers designed according to the conserved sequences of mcp gene of iridoviruses, then the flaking sequences adjacent to the core region were amplified by inverse pcr, and the complete sequence was obtained by combining all of them. the open reading frame of the gene is 1380bp in length, encoding a putative ... | 2007 | 17969861 |
| a new genotype of lymphocystivirus isolated from cultured gilthead seabream, sparus aurata l., and senegalese sole, solea senegalensis (kaup). | | 2010 | 20487141 |
| the genomic diversity and phylogenetic relationship in the family iridoviridae. | the iridoviridae family are large viruses (~120-200 nm) that contain a linear double-stranded dna genome. the genomic size of iridoviridae family members range from 105,903 bases encoding 97 open reading frames (orfs) for frog virus 3 to 212,482 bases encoding 211 orfs for chilo iridescent virus. the family iridoviridae is currently subdivided into five genera: chloriridovirus, iridovirus, lymphocystivirus, megalocytivirus, and ranavirus. iridoviruses have been found to infect invertebrates and ... | 2010 | 21994690 |