| the replication and titration of iridescent virus type 22 in spodoptera frugiperda cells. | a plaque assay for iridescent virus type 22 (from simulium sp.) using spodoptera frugiperda cells has been devised, and the kinetics of growth of the virus in this cell line have been determined. the virus particle/p.f.u. ratio was 75 +/- 8, and the p.f.u./tcid50 ratio was 0.56 +/- 0.11. | 1978 | 621497 |
| infectivity and pathogenesis of iridescent virus type 22 in various insect hosts. | this paper reports the results of a series of laboratory experiments to determine the infectivity and pathogenesis of iridescent virus type 22 (iv 22) for six species of mosquitoes, phlebotomine sand flies and triatomid bugs. following inoculation, iv 22 replicated in all of the species tested, without producing noticeable mortality within a 14 day observation period. examination of the infected insects by immunofluorescence demonstrated large amounts of viral antigen in many different organs. e ... | 1992 | 1355961 |
| identification and characterization of the gene encoding the major structural protein of insect iridescent virus type 22. | the major structural protein (msp--apparent molecular weight 49,000) of insect iridescent virus type 22 (isolated from blackflies--simulium spp.) was resolved from disrupted, purified virus particles by sds-page and transferred to nitrocellulose by western blotting. the portion of the blot containing the msp was identified and excised. tryptic peptides, generated by digestion in situ, were purified by hplc. three of these peptides were sequenced and an oligonucleotide gene probe was designed usi ... | 1990 | 2389558 |
| iridescent virus type 22 dna. | double stranded dna extracted from iridescent virus type (iv 22) was characterized by its buoyant density in cscl, thermal denaturation profile and guanine plus cytosine content. the dna was linear with a molecular weight of 130--142 x 10(6) determined by reassociation kinetics, contour length measurements and restriction endonuclease analysis. | 1981 | 6264894 |
| is the major capsid protein of iridoviruses a suitable target for the study of viral evolution? | iridoviruses are large cytoplasmic dna viruses that are specific for different insect or vertebrate hosts. the major structural component of the non-enveloped icosahedral virus particles is the major capsid protein (mcp) which appears to be highly conserved among members of the family iridoviridae, phycodnaviridae, and african swine fever virus. the amino acid sequences of the known mcps were used in comparative analyses to elucidate the phylogenic relationships between different cytoplasmic dna ... | 1998 | 9562891 |