| analysis and characterization of the complete genome of tupaia (tree shrew) herpesvirus. | the tupaia herpesvirus (thv) was isolated from spontaneously degenerating tissue cultures of malignant lymphoma, lung, and spleen cell cultures of tree shrews (tupaia spp.). the determination of the complete nucleotide sequence of the thv strain 2 genome resulted in a 195,857-bp-long, linear dna molecule with a g+c content of 66.5%. the terminal regions of the thv genome and the loci of conserved viral genes were found to be g+c richer. furthermore, no large repetitive dna sequences could be ide ... | 2001 | 11312357 |
| identification and characterization of the tupaia herpesvirus dna polymerase gene. | tupaia herpesviruses (thvs) have been isolated from malignant lymphomas and from degenerating lung or spleen cell cultures of tree shrews (tupoia spp.), but because of a lack of genetic information the final classification of thvs is still open. in the present work the viral dna polymerase (dpol) gene was mapped within the genome of the different thv strains using pcr and degenerate oligonucleotide primers. nucleotide sequences of the dpol genes of thv strains 1 to 5 were determined and used for ... | 1998 | 9880021 |
| analysis of tupaia herpesvirus proteins by one- and two-dimensional gel electrophoresis. | the polypeptide composition of four tupaia herpesvirus strains--of which two were isolated from malignant tumors--was analysed by one- and two-dimensional gel analysis. it was found that purified virions of the tree shrew consist of a least 37 viral polypeptides when determined by sds polyacrylamide gel electrophoresis. the number of viral polypeptides increased to 49 when analysed by the two-dimensional technique with the molecular weights ranging from 12.000 to 230.000. variations in the viral ... | 1982 | 7166203 |
| analysis of polypeptides of the tree shrew (tupaia) herpesvirus by gel electrophoresis. | the virion polypeptide composition of three independently isolated tree shrew herpesviruses (thv) was analysed by sds-polyacrylamide slab gel electrophoresis and by a two-dimensional technique using isoelectric focusing. two of the virus isolates analysed were from malignant tumours; the other isolate (thv, strain 1) was from an apparently healthy animal. the polypeptide patterns of the three purified tupaia herpesvirus isolates were remarkably similar, each consisting of at least 35 polypeptide ... | 1982 | 7142964 |
| structural organization and analysis of the viral terminase gene locus of tupaia herpesvirus. | tupaia herpesvirus (thv) was isolated from spontaneously degenerating tissue cultures of malignant lymphoma, lung, and spleen cell cultures of tree shrews (tupaia spp.). in order to determine the phylogenetic relatedness of thv the complete nucleotide sequence of the viral terminase (vter) gene locus (6223 bp) of tupaia herpesvirus strain 2 (thv-2) was elucidated and analysed. the vter gene locus, encoding one of the most highly conserved herpes viral proteins is composed of two exons. the intro ... | 2001 | 11226571 |
| structural organization of a conserved gene cluster of tupaia herpesvirus encoding the dna polymerase, glycoprotein b, a probable processing and transport protein, and the major dna binding protein. | the tupaia herpesviruses (thvs) have been isolated from malignant lymphoma tissue cultures and from degenerating lung and spleen cell cultures of tree shrews (tupaia spp.). recently we succeeded in the localization of the gene locus of the thv dna polymerase (dpol) gene within the viral genome. based on these results the highly conserved gene cluster of herpesviruses encoding the dpol, the glycoprotein b (gb), a probable processing and transport protein (prtp), and the major dna binding protein ... | 1999 | 10392721 |
| protein kinase and specific phosphate acceptor proteins associated with tupaia herpesvirus. | a protein kinase activity was found to be associated with tree shrew (tupaia) herpesvirus. the protein kinase was characterized with respect to its requirements for enzymatic activity. a divalent cation such as mg2+ or mn2+ was necessary as well as atp as the phosphate donor. distinct tupaia herpesvirus polypeptides (molecular weights of 100,000 [100k], 82k, and 53k) were found to be phosphate acceptor proteins when 5 mm mg2+ was used. at a higher mg2+ concentration (20 mm), additional viral pro ... | 1982 | 6809957 |