the specificity of heterophil antibodies in patients and healthy donors with no or minimal signs of infectious mononucleosis.over several years sera were collected from 14 heterophil-positive students or patients who did not fulfill minimal hematologic criteria for infectious mononucleosis (i.m.) the specificity of these heterophil reactions for i.m. was investigated by determining antibodies to epstein-barr virus-determined antigens, i.e., to viral capsid antigens (vca), early antigens (ea), and ebv-associated nuclear antigens (ebna). on the basis of detectable anti-ea and/or the early absence and late emergence of a ...19761126
comparison between two antibody populations in the ebv system: anti-ma versus neutralizing antibody activity.ebv-neutralizing antibody titers were determined in 11 sera derived from african burkitt lymphoma or nasopharyngeal carcinoma patients and in the corresponding serum fractions retained above xm 100 diaflo membranes after low ph treatment, and after recombination of the retained and passed fractions by neutralization of the acidified samples. they were compared with the corresponding antimembrane antigen (ma) titers in seven of the same sera. while all sera tested showed substantial increase of t ...19762559
studies of an epstein-barr virus-induced dna polymerase. 197714436
tumorigenicity of human hematopoietic cell lines in athymic nude mice.human hematopoietic cell lines, which had been classified on the basis of studies on clonality, and morphological, chromosomal and functional parameters as lymphoblastoid cell lines (lcl) of presumed non-neoplastic origin, and lymphoma, myeloma and leukemia lines of proven malignant origin, were tested for tumorigenic potential on subcutaneous transplantation to nude mice and for capacity to grow in semi-solid medium in vitro. recently established lcl failed to grow both in nude mice and in agar ...197714896
studies on epstein-barr virus-related antigens. ii. biochemical properties of soluble antigen in raji burkitt lymphoma cells.biochemical properties of epstein-barr virus (ebv)-related soluble antigen in non-producer raji burkitt lymphoblastoid cells, assayed by the indirect single radial immunodiffusion (isrd) test, were investigated. the soluble isrd antigen retained activity even after exposure to a temperature of 80 degrees c. the antigen was precipitated in 40% saturated ammonium sulfate and isrd activity was recovered from the precipitate when reconstituted into a solution. isoelectric focusing and crossed immuno ...197714897
appearance and some unusual features of epstein-barr virus antibody production in infectious mononucleosis and other health disorders.the indirect immunofluorescence (if) test and the complement-fixation reaction (cfr) were used in examination of over 1500 sera obtained from patients with infectious mononucleosis (im) and other health disorders. the evidence obtained supports a direct aetiological relationship between epstein-barr virus (ebv) and im and points on a relationship of ebv to some other lymphadenopathies and health disorders. the incidence of the igg type antibody against virus capsid antigen (eb-vca) and soluble a ...197715437
late persistence of serum gamma-glutamyl transpeptidase activity after mononucleosis. report of 3 cases.gamma-glutamyl transpeptidase (ggtp) is a sensitive but nonspecific index hepatobiliary disease. in infectious mononucleosis (im) or the mononucleosis-like disease attributable to cytomegalovirus (cytomegalovirus-induced im), ggtp reverted to normal later than aspartate aminotransferase and alkaline phosphatase. in three cases elevated serum ggtp activity persisted for up to 24 months -- raising the question of persistent 'post-im' hepatitis. such prolonged ggtp activity was unusual in other lat ...197715921
differential expression of d and r components of epstein-barr virus early antigen after superinfection and after induction with 5-iododeoxyuridine.three non-productive human lymphoblastoid cell lines, nc-37, raji and l-1, either superinfected with epstein-barr (eb) virus from p3hr-1 cells or treated with 5-iododexyuridine (iudr), were studied for the development of early antigen (ea) of eb virus. in all three superinfected cell lines, both the restricted (r) and diffuse (d) componets of ea were synthesized, with r-component formation preceding d-component synthesis. after iudr treatment both components of ea were only formed in raji cells, ...197718915
opportunistic non-hodgkin's lymphoma in x-linked recessive immunodeficiency and lymphoproliferative syndromes. 197721454
mosquito-virus relationships of american encephalitides. 197824407
epstein-barr virus infection following bone-marrow transplantation.a 12-year-old patient with acute lymphoblastic leukemia received a bone-marrow transplant (bmt) from a matched sibling donor. nine weeks prior to transplant the donor experienced epstein-barr virus (ebv)-induced infectious mononucleosis. the bone-marrow recipient was ebv-negative at the time of transplant; however, 4 weeks post transplant the recipient developed clinical symptoms of graft-verus-host disease (gvhd) coincident with serological evidence of acute ebv infection. in addition, a lympho ...197829839
immunovirological examination of patients with lymphoblastic and prolymphocytic lymphosarcomas. 197830234
uv-inactivation of epstein-barr virus: differences in early antigen expression in two different non-productive cell lines and influence of caffeine.two non-productive epstein-barr (eb) virus genome-carrying lymphoblastoid cell lines, namely raji and nc37, were used for studying the effect of uv irradiation on the ability of p3hr-1 eb virus to induce early antigen (ea) formation. in nc37 cells infected with uv-irradiated virus the formation of ea was delayed; thus the slope of inactivation curve based on the early (24 hr) reading was steeper than that based on the late (72 hr) reading. this was not observed in raji cells. caffeine did not in ...197830263
intracranial heterotransplantation of human hematopoietic cells in nude mice.a panel of established cell lines and many primary cell specimens from lymphomas and leukemias as well as from normal lymphatic tissues were tested for tumorigenicity by intracranial heterotransplantation in nude mice. not only lymphoma and leukemia cell lines, but also lymphoblastoid cell lines, lacking markers of malignancy, were tumorigenic in the brains of nude mice. these findings indicate that tumorigenicity following intracranial heterotransplantation in nude mice cannot be used as proof ...197938211
epstein-barr virus infections in childhood.igm and igg antibodies against epstein-barr virus (ebv) capsid antigen and antibodies against ebv nuclear antigen and heterophil antibodies were investigated in 115 paired sera of children with acute infections and in 100 sera of healthy controls of the same age and sex. ebv-specific igm antibodies could be recognized in 13.7% of the patients and in 7% of the controls. antibodies against ebv nuclear antigen were not detected in the igm-positive sera.197939436
enhancement of epstein-barr virus antibody production in systemic lupus erythematosus patients.two groups of 22 patients suffering from either systemic lupus erythematosus (sle) or infectious mononucleosis (im) were checked for epstein-barr virus capsid antigen antibody (eb-vca) production. the average significant antibody levels as well as the frequency of their occurrence were clearly higher in sle than in im patients.197939442
abortive growth of human lymphocytes carrying a dormant epstein-barr viral genome.when p3hr-1 lymphoblastoid cells expressing epstein-barr virus (ebv)-related antigens at very low frequency were cocultivated with human umbilical cord blood lymphocytes and the cell-free mixed culture fluid was applied to fresh cord lymphocytes, cells morphologically distinct from normal lymphocytes became evident after one to two weeks' exposure. the abnormal cells became abundant after one month and were easily identified by b-cell markers and a variety of morphologic abnormalities. such abno ...197845600
blastogenic response and ebna induction in human lymphocytes by epstein-barr virus only requires b cells but not macrophages.the postulated role of macrophages in the primary infection of human lymphocytes by epstein-barr virus (ebv) was examined. macrophage removal had no effect on the blastogenic response but slightly reduced the percentage of ebna-positive cells induced by the b 95-8 virus strain. both the rate of dna synthesis and the appearance of ebna-positive cells could rather be related to the number of b cells in the infected populations. macrophages are thus not required for the initiation of ebv-induced ly ...197745722
letter: infectious mononucleosis, academic performance, and emotional stability. 197546553
epstein-barr virus-induced transformation of human leukocytes after cell fractionation.the efficiency of transformation of human lymphocytes after infection with epstein-barr virus (ebv) was determined in fractionated and non-fractionated preparations derived from 16 human cord blood samples and two blood samples from adult donors. the transformation efficiency of macrophage-depleted leukocytes was consistently lower as compared to non-fractionated leukocytes. additional depletion of b-cells resulted in a further decrease. reduction of t-cells, however, did not influence significa ...197547842
infectious mononucleosis: model for self-limiting lymphoproliferation.evidence for the extensive lymphoproliferation which characterises infectious mononucleosis (i.m.) is summarised, and some of the mechanisms which are though to control lymphoproliferative activity in this disease are discussed. the main host responses which appear to limit lymphoproliferation in i.m. include the development of humoral antibodies against the epstein-barr (e.b.) virus-associated membrane antigen (present on the e.b. viral envelope and on e.b.-virus-infected cells) and cellular im ...197548066
x-linked recessive progressive combined variable immunodeficiency (duncan's disease).of 18 boys in duncan kindred, 6 died of a lymphoproliferative disease. they exhibited a subtle, progressive combined variable immunodeficiency disease characterised by benign or malignant proliferation of lymphocytes, histiocytosis, and alterations in concentrations of serum-immunoglobulins. infectious mononucleosis occurred during or preceding terminal events in at least 3 of the cousins. fever, pharyngitis, lymphadenomegaly, hepatosplenomegaly, atypical lymphocytosis, and a spectrum ranging fr ...197548119
lymphoblastoid transformation and kinetics of appearance of viral nuclear antigen (ebna) in cord-blood lymphocytes infected by epstein-barr virus (ebv).human cord-blood lymphocytes were infected with b95.8 epstein-barr virus (ebv) before and after separation into b- and t-cell populations. lymphoblastoid cells exhibiting b-cell characteristics appeared after 2 to 3 days of culture in the total population and in the separated b-cell subpopulation but not in the t-cell subpopulation. ebv nuclear antigen (ebna) was detected concurrently with the appearance of lymphoblastoid cells. the proportion of ebna-positive cells corresponded to that of lymph ...197549325
tumor viruses: 1974. 197550896
the epstein-barr virus and malignancy: molecular evidence. 197550897
hodgkin's disease. 197551322
mononucleosis-associated subacute sclerosing panencephalitis.three cases of coincidental infectious mononucleosis (i.m.) and subacute sclerosing panencephalitis (s.s.p.e.) in children are reported. in two of the cases, i.m. preceded the appearance of s.s.p.e. symptoms. the other case of s.s.p.e. was associated with apparent mononucleosis encephalitis. all three patients died within 3 months of s.s.p.e. being diagnosed and had pathological changes characteristic of that disease and raised levels of measles antibody. two of the patients had apparent t-cell- ...197551346
clinical and serological analysis of transfusion-associated hepatitis.of 108 prospectively followed, multiply transfused, open-heart-surgery patients, 12 (11%) developed hepatitis. patients received only volunteer donor blood tested for hepatitis-b surface antigen (hbsag) prior to transfusion by counterelectrophoresis (c.e.p.). 4 of the 12 patients developed hepatitis-b-virus infection. subsequent testing of donor serums by solid-phase radioimmunoassay (r.i.a.) revealed that an r.i.a.-positive, c.e.p.-negative blood unit was transfused to 3 of the 4 type-b hepatit ...197553329
critical evaluation of b and t cell markers in man. 197553874
ebv-determined nuclear antigen (ebna)-positive cells in the peripheral blood of infectious mononucleosis patients.after removal of srbc rosette-forming t-cells from the peripheral blood, the residual, largely b-lymphocyte fraction of five infectious mononucleosis patients was found to contain 0.5-2% blast cells, positive for the ebv-determined nuclear antigen (ebna). there was a rough parallelism between the presence of large lymphoblasts in the hematological smear, ebns-positive large blasts in the b-cell fraction and the ability of the t-cell fraction to exert an ebv-specific lymphocytotoxicity on establi ...197655396
persistence of epstein-barr viral nuclear antigen (ebna) in cells entering the eb viral was shown by double immunofluorescence studies that epstein-barr viral nuclear antigen (ebna) was preserved in ebv-infected cells after they had entered the productive viral cycle, as signalled by the appearance of the early antigen (ea)complex. a nuclear component of the ea comples could be clearly distinguished from ebna with regard to antigenic specificity.197655398
lymphocyte transformation induced by autologous cells. iii. lymphoblast-induced lymphocyte to stimulation does not correlate with eb viral antigen expression or immunity.human mitogen-induced and cell line b lymphoblasts stimulate the proliferation of allogeneic and autologous lymphocytes in culture. the role in thes reaction of eb viral determinants on the stimulating cells and immunity of the lymphocyte donor to the eb virus has been studied. the stimulatory capacity of cultured cell line lymphoblasts is not inhibited by incubating lymphoblasts with antisera to eb viral determinants. cultured cell line b lymphoblasts stimulate as much thymidine incorporation b ...197655437
immunological control of human lymphoma: discussion. 197656225
the absence of b-cell antigen b2 from leukaemia cells and lymphoblastoid cell lines.60 leukaemia patients with acute mylogenous leukaemia, chronic myelocytic leukaemia, acute lymphatic leukaemia, and chronic lymphocytic leukaemia were tested for five specificities of the new b-lymphocyte alloantigenic system. two specificities of the first b-cell locus were significantly lower in frequency than in b lymphocytes from 105 controls. b-cell group 1 was found in 18% of leukaemia patients vs. 48% of controls (p less than 0.025) and b-cell group 2 was found in 0% of leukaemia patients ...197659011
somatic cell hybrids between human lymphoma lines. ii. spontaneous and induced patterns of the epstein-barr virus (ebv) cycle.the regulation of spontaneous, iudr-induced and p3hr-1 virus-induced ea and vca production patterns was studied in two new somatic hybrids between human lymphoma lines. the hybrid 8a was derived from the crossing of the non-producer raji with the spontaneous producer daudi line. the second hybrid, 83, was produced by the fusion of raji with the ebv-genome-negative b-lymphoma line, bjab. the studies suggest the following ebv regulation patterns: (1) the spontaneous production of ea and vca appear ...197659708
surface markers on human b and t lymphocytes. viii. association between complement and epstein-barr virus receptors on human lymphoid association has been found between the epstein-barr virus (ebv) and complement (c3d) receptors on human lymphoid cells. the evidence was four-fold: there was a correlation between the expression of these two receptors; inhibition experiments showed that the binding sites probably are close to each other in the cell membrane, although not identical; ebv and complement receptors have been found to co-cap in either order; and lymphoid cell lines lacking complement receptors could not be superinf ...197659946
oncogenic viruses: implications for human diseases (part ii). 197660244
molecular probes for tumor viruses in human cancer. 197660297
[virus etiology of lymphomas and leukemias in man]. 197661149
studies on epstein-barr virus-related antigens. i. indirect single radial immunodiffusion as a useful method for detection and assay of soluble antigen.a useful method for the detection and assay of epstein-barr virus (ebv)-related soluble antigen has been developed by the application of the indirect single radial immunodiffusion technique which is frequently used for quantitative measurements of immunoglobulins and other soluble proteins. when the extracts of ebv-determined nuclear antigen (ebna)-positive non-producer cells (raji and nc-37) were applied to agar plates containing seropositive human serum, followed by overlay with anti-human igg ...197661945
pathogenesis and phenotypes of an x-linked recessive lymphoproliferative syndrome.a new x-linked recessive lymphoproliferative syndrome has variable phenotypes: fatal infectious mononucleosis (i.m.), agammaglobulinaemia after i.m., american burkitt's lymphoma, histiocytic lymphoma, immunoblastic sarcoma of b cells, or plasmacytoma. an immunodeficiency to rubeola and the epstein-barr virus probably ensues from the mutant gene. the phenotypes (spectrum of b-cell disorders) have a common inheritance and the aetiology is similar.197662116
subpopulations of human lymphoblastoid cell lines. correlation with the expression of surface receptors and content of epstein-barr virus genome.forty lymphoblast cell lines derived from normal subjects, patients with infectious mononucleosis, leukemia, and burkitt's lymphoma have been studied for surface receptors including surface ig, complement receptors by the eac rosette and fluorescent (raji cell) techniques, and fc (aggregate) receptor by direct and indirect immunofluorescence. because of the b-cell tropism of the epstein-barr virus (ebv), an effort was made to correlate the expresion of various surface properties of lymphoblastoi ...197662390
inducibility of the epstein-barr virus (ebv) cycle and surface marker properties of ebv-negative lymphoma lines and their in vitro ebv-converted sublines.two ebv-negative lymphoma lines of human b-cell origin, bjab and ramos, were compared with altogether six of their in vitro ebv-converted, ebna- and ebv-dna-carrying sublines (four of ramos and two of bjab derivation). all converted lines closely resembled the parental line with regard to karyotype and hl-a and b antigen typing. induction of ebv antigens (ea and vca) by p3hr-1 virus superinfection was either similar in the converted and the negative lines, or somewhat increased in certain conver ...197662724
aetiology of nasopharyngeal carcinoma. 197663856
detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their fibroblasts exposed to cytosine arabinoside (ara c) were infected with either cytomegalovirus (cmv) or herpes simplex virus (hsv) type 1 and 2. herpesvirus-determined early antigens (hv-ea), detected by anti-complement immunofluorescence (acif), occurred primarily in the nucleic, and the specificity of these results was established by an acif blocking reaction using f(ab')2 fragments of human and hyperimmune reference sera. direct tests with selected sera and cross-blocking experiments betw ...197764461
is burkitt's lymphoma related to perinatal infection by epstein-barr virus?burkitt's lymphoma (b.l.) is very restricted geographically, whereas the epstein-barr virus (e.b.v.), a very likely causal factor, exists all over the world. it is proposed that perinatal infection (either transplacental, or, more probably, neonatal) is an important risk factor for the development of b.l. this hypothesis arose when the epidemiological characteristics of e.b.v. infection were compared in different parts of the world and is supported by the preliminary results of a seroepidemiolog ...197764861
complex pathogenesis of cancer in transplant recipients. 197765239
comparison of dna polymerase activities from an american burkitt's lymphoma cell line and ebv producer and nonproducer cells. 197565315
comparative studies on ebv antigens by immunofluorescence and immunoperoxidase techniques.three groups of ebv antigens, vca, ma and ea, were compared by the techniques of electron microscopic immunoperoxidase (ip) and immunofluorescence (if). p3hr-1 and ebv superinfected raji cells served as targets for labelled sera from patients with bl, npc and im or from healthy donors. 125i peroxidase-labelled antibodies were also prepared to determine, autoradiographically, the penetration of the complex into the cell system, and to monitor the incubation and washing procedures. the development ...197766208
separation of the complement-fixing and early antigens from epstein-barr soluble antigen.the epstein-barr virus-associated complement-fixing (soluble) antigen (cfsa) and the early antigen (ea) were isolated by ion-exchange chromatography from the p3hr-1 lymphoblastoid cell line activated with 5-iodo-2'-deoxyuridine for 72 hours. as expected, these two antigens were not immunologically identical. the cfsa, but not the ea, was isolated from the rajl lymphoblastoid cell line by the same method.197766320
an immunofluorescence technique with counterstain on fixed cells for the detection of antibodies to human herpesviruses; antibody patterns in patients with hodgkin's disease and nasopharyngeal indirect immunofluorescence (if) test on fixed cells with evans' blue counterstain is described for all four human herpesviruses, i.e., herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2), varicella-zoster virus (vzv), cytomegalovirus (cmv) and epstein-barr virus (ebv). comparison with immunodiffusion (id) for hsv-2 and with id and complement fixation (cf) for vzv and cmv demonstrated the specificity and high sensitivity of the if test. also introduced is a modification of the anti-compl ...197667099
meralgia paraesthetica and epstein-barr virus. 197767330
prospective study of epstein-barr virus antibody tetres and survival in patients with nasopharyngeal carcinoma. 197767401
identification of a purified complement-fixing antigen as the epstein-barr-virus determined nuclear antigen (ebna) by its binding to metaphase chromosomes.a soluble complement-fixing antigen carried by epstein-barr virus (ebv)-transformed human cells has been previously extracted from cell nuclei and purified by dna-cellulose chromatography [luka, j., siegert, w. & klein, g. (1977) j. virol., in press]. on addition of this antigen to methanol/acetic acid-fixed metaphase chrmosomes, followed by exposure to human sera containing antibodies against the ebv-determined nuclear antigen (ebna), brilliant positive staining was obtained by anti-complement ...197767603
quantitation of beta2-microglobulin and hla on the surface of human cells. ii. in vitro cell lines and their hybrids.beta2-microglobulin (beta2m) participates as an integral part in molecules of the major histocompatibility complex (mhc) type. absence of beta2m makes the residual heavy mhc chain largely inactive as antigen. striking reductions in the density per unit surface area of beta2m in seven out of nine malignant lymphoid tumour lines in comparison with normal lymphocytes or "immortalized" epstein-barr-virus-transformed lymphoblastoid lines were found is this study. this would seemingly represent a spec ...197767633
characterization of ebv-genome negative "null" and "t" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-hodgkin lymphoma.sixty-two explants from peripheral blood, bone marrow and cerebral fluid of children with acute lymphoblastic leukemia (all) and leukemic transformed non-hodgkin lymphoma (nhl) were cultivated for at least 8 weeks. although lymphatic cells persisted up to 16 weeks in tissue culture, no proliferation was observed in 54 cultures. from the remaining cultures, eight permanently growing cell lines were obtained. five of these were ebna (epstein-barr virus-specific nuclear antigen)-positive. three, ho ...197768013
unusual prevalence of antibodies to epstein-barr virus early antigen in ataxia telangiectasia. 197768263
antibody-dependent cellular cytotoxicity (adcc) against epstein-barr virus-determined antigens. iii. reactivity in sera from patients with burkitt's lymphoma in relation to tumour development.sera from patients with burkitt's lymphoma (bl) were tested for antibody-dependent cellular cytotoxicity (adcc) against epstein-barr virus (ebv) determined antigens and for antibodies directed against various ebv-specific antigens. no correlation was found between ebv serology and adcc in sera selected for high or low titres against the membrane antigens (ma) or in consecutive sera from three patients with isolated late tumour recurrences. furthermore, no correlation was found between adcc and t ...197770292
cytomegalovirus-directed lymphocyte reactivity in healthy adults tested by a cmv-induced lymphocyte transformation test.a cytomegalovirus (cmv) induced lymphocyte tranformation test (ltt) performed as a microtechnique assay is described. investigations were done in a group of twenty-five healthy adults with normal lymphocyte reactivity to phytohaemagglutinin and previously encountered antigens. lymphocyte reactivity was established in cmv-seropositive persons using heat-inactivated cell-free cmv strain ad 169 in an optimal stimulating dose of 10(1-5) tcid 50. positive cmv-ltt results were shown to be induced by p ...197770294
terminal deoxynucleotidyl transferase activity and cell surface antigens of two unique cell lines (nalm-1 and balm-2) of human leukemic origin.two unique cell lines, nalm-1 and balm-2 derived from lymphoblast-like cells of chronic myelogenous leukemia and rare b cell acute lymphoblastic leukemia patients, respectively, were compared with fresh parent cells from the patients and with a philadelphia chromosome positive k-562 cell line previously established from a chronic myelogenous leukemia patient in blastic phase. nalm-1 resembled the parent cells in the presence of philadelphia chromosome, non-t/non-b acute lymphoblastic leukemia sp ...197770413
observations on the antigenic relationships between epstein-barr virus and herpesvirus saimiri.the antigenic relationships between epstein-barr (eb) virus and herpesvirus saimiri (hvs) have been investigated in comparative immunofluorescence, microimmunodiffusion and serum neutralization tests. no similarity was detected between the structural antigens of the two herpesviruses but the results of microimmunodiffusion tests showed that they shared a non-structural, virus-determined antigen. the implications of this finding are discussed in relation to the importance of hvs and its monkey ly ...197770507
continuous lymphoblastoid suspension cultures from cells of haematopoietic organs of baboons with malignant lymphoma. report iii: immunological studies.lymphoblastoid cultures established from haematopoietic organs of baboons with lymphoma belong to the b-cell type. cytoplasmic antigen is revealed in 2--6% of suspension culture cells established from lymphomatous baboons by indirect immunofluorescence reaction using serum from human burkitt's lymphoma patients with antibody titer to capsid antigen of epstein-barr virus 1: 320. there is a correlation between the number of antigen-containing cells in these cultures and the number of cells in whic ...197771245
characterization of lymphoid cell lines established from multiple epstein-barr virus (ebv)-induced lymphomas in a cotton-topped marmoset. 197771273
multiple-sclerosis-associated agent. 197771613
[virus and cancer]. 197771664
detection of epstein-barr virus antigens with enzyme-conjugated antibody.monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ...197772045
human b cell line deficient in the expression of b cell-specific glycoproteins (gp 27,35).a human b lymphoid cell line, p3hr-1, expresses only low levels of the 27 000 and 35 000 mol.wt. b cell-specific glycoproteins (gp 27,35). indirect antibody-binding and quantitative absorption tests with a xenoantiserum against the antigens showed that p3hr-1 cells have on their surface about 1% of the amount found on other human b lymphoblastoid cell lines. the deficit of the glycoproteins on the surface of p3hr-1 cells could be accounted for by a reduced rate of synthesis in these cells. a sim ...197772671
radioimmunoassay for epstein--barr virus (ebv)-associated nuclear antigen (ebna). binding of iodinated antibodies to antigen immobilized in polyacrylamide gel. 197773461
detection of a nuclear, ebna-type antigen in apparently ebna-negative herpesvirus papio (hvp)-transformed lymphoid lines by the acid-fixed nuclear binding agreement with the findings of previous authors, we could not detect a virally determined nuclear antigen in herpesvirus papio (hvp)-transformed baboon lymphoid lines by anticomplementary staining in situ, as for ebna. however, by means of our recently developed acid-fixed nuclear binding technique an ebna-like antigen could be readily demonstrated, after extraction from both producer and non-producer lines. we propose to designate the antigen as hupna. it can be detected by a human anti-ebna ...197773523
epstein barr virus and nasopharyngeal carcinoma. 197673653
pathogenesis of infectious mononucleosis. 197773962
epstein-barr virus induces viral nuclear antigen in nasopharyngeal epithelial cells. 197773981
effects of 5-tungsto-2-antimoniate in oncogenic dna and rna virus-cell systems. 197774337
serum iga antibodies to epstein-barr virus capsid antigen preceding symptoms of nasopharyngeal carcinoma. 197875457
production of rabbit antibodies against the viral capsid antigen (vca) of the epstein-barr virus (ebv).rabbits were immunized with nuclear or cytoplasmic extracts of the epstein-barr virus (ebv)-producing marmoset cell line b95-8. following extensive absorption with human ebv-negative cells (hep-2, ramos and bjab), sera were obtained that no longer reacted with cellular or serum proteins of human origin, but gave a single precipitin band with extracts of the human ebv-producing line, p3hr-1. immunofluorescence tests performed with appropriate parallel human serum controls showed that the rabbit s ...197875856
transmission of non-a, non-b hepatitis from man to chimpanzee.non-a, non-b hepatitis was transmitted to four colony-born chimpanzees by intravenous inoculation of human sera. two chimpanzees were inoculated with serum from a patient with a clinical and serological diagnosis of chronic non-a, non-b hepatitis whose blood appeared to transmit this disease to a nurse following accidental needle-stick, and the other two chimpanzees were inoculated with serum from either of two former blood-donors whose hbsag-negative blood appeared to transmit clinically recogn ...197876018
epstein-barr virus infection in staff treating patients with nasopharyngeal carcinoma. 197876242
non-a, non-b hepatitis. 197877141
epstein-barr-specific iga in nasopharyngeal cancer. 197878104
observations on cell lines derived from a patient with hodgkin's disease.permanent cell lines have been established from a spleen nodule and lymph node of a male hodgkin's disease (hd) patient whose father has the same disease. th in vitro growth pattern morphological and cytogenetic characteristics of these lines maintained continuously for over 2 years are described. the cultures contain a population of mixed cell types that grow in suspension. between 5 and 10% of the cells have surface immunoglobulins m and d. b-cell alloantigens are also detectable. while the cu ...197878764
colonies of ebna-positive cells in soft agar from peripheral leukocytes of infectious mononucleosis patients.epstein-barr virus (ebv)-associated nuclear antigen (ebna)-positive lymphoblastoid cells grew as colonies in soft agar after seeding of leukocytes from the peripheral blood of four patients with infectious mononucleosis serologically determined to be caused by ebv. in individual cases more colonies were obtained from blood specimens during the acute phase of the disease than during the convalescent phase. incorporation of human umbilical cord serum, which contained neutralizing antibody to ebv, ...197878908
ebv antigens in lymphocytes of patients with exudative tonsillitis, infectious mononucleosis and hodgkin's disease.a protocol utilizing isokinetic gradients to isolate human lymphocytes is combined with another that purifies the c3 receptor-bearing b lymphocyte subpopulation, thus enriching the eb virus genome-carrying population. also, rabbit antisera were prepared to the epstein-bar virus nuclear antigen (ebna) and the early antigen (ea) and utilized in an indirect immunofluorescence test (iit) to detect these antigens in human lymphocytes isolated from various disease states. using these methods we demons ...197878913
persistent epstein-barr virus infection in a child with hypergammaglobulinaemia and immunoblastic proliferation associated with a selective defect in immune interferon secretion.a 5-year-old girl had a chronic disease characterised by fever, lymphoid hyperplasia, interstitial pneumonitis, thrombocytopenia, and polyclonal hypergammaglobulinaemia. evidence for severe, persistent epstein-barr virus (e.b.v.) infection was found: titres of antibody to e.b.v. viral capsid antigen (igm and igg) and early antigen were extremely high, cells containing e.b.v.-associated nuclear antigen (e.b.n.a.) were found in lymph nodes and blood, and spontaneous permanent lymphoblastoid cell l ...197879029
epstein-barr virus and burkitt lymphoma. 197879925
heterogeneity of epstein-barr virus. iv. induction of a specific antigen by ebv from two transformed marmoset cell lines in ramos cells.infection of cells of the ebv-genome-negative human b-lymphoma ramos line with viral isolates obtained from two ebv-transformed marmoset cell lines (b95/8; nyevu) resulted in the induction of a nuclear antigen (ram-ag) apparently different from other ebv-associated antigen complexes. this antigen is revealed by indirect immunofluorescence and shows no detectable cross-antigenicity with ebna or any other known ebv-associated antigen. ebv-isolates from p3hr-1 cells fail to induce a similar antigen ...197881181
cellular immunity in infectious mononucleosis. ii. specific reactivity to epstein-barr virus antigens and correlation with clinical and hematologic parameters.infectious mononucleosis (im) patients, epstein-barr virus (ebv)-seropositive and seronegative healthy donors, and patients with other viral infections were tested for lymphocyte blastogenesis (lb) with phytohemagglutinin and six ebv (virus concentrate, culture supernatant, and soluble [s] antigen) or control antigens. fluorescent antibodies to ebv viral capsid antigen of igg, igm, iga specificities, to nuclear antigen (ebna), and heterophile antibodies were also assayed. these were correlated w ...197881224
papain solubilization of the epstein-barr virus-induced membrane antigen.the epstein-barr virus (ebv)-induced membrane antigen (ma) was successfully solubilized from the membranes of viable ebv-infected raji cells by treatment with papain (5 to 6 u per 1 x 10(7) to 2 x 10(7) cells). the loss of ma from viable cells was monitored by membrane immunofluorescence and antibody-dependent cellular cytotoxicity. soluble ma was demonstrated in papain digests through inhibition of antibody-dependent cellular cytotoxicity and by inhibition of the binding of anti-ma antibodies t ...197881320
epstein-barr antibodies in suspected nasopharyngeal carcinoma. 197882106
epstein-barr virus d.n.a. in tumour tissue from native alaskan patients with nasopharyngeal carcinoma. 197882107
longitudinal study of epstein-barr virus antibody titers and excretion in pediatric patients with hodgkin's disease.nineteen pediatric patients with hodgkin's disease (hd) who had experienced primary epstein-barr virus (ebv) before, or in one case after, diagnosis, were studied longitudinally for changes in the titers and spectra of ebv-related antibodies, excretion of ebv into the oropharynx, the number of ebv-carrying lymphoid cells in the peripheral blood, and clinical signs and symptoms suggestive of reactivation of the latent virus. the incidence and geometric mean titers of igg antibodies to viral capsi ...197882539
study of epstein-barr virus-determined nuclear antigen (ebna) by chromatography on fixed cell nuclei.low ionic strength (50 to 100 mm nacl) and ph 6.0 were found to be optimal conditions for in vitro conversion of epstein-barr virus (ebv)-determined nuclear antigen (ebna)-negative nuclei to ebna-positive nuclei by addition of the complement-fixing (cf) antigen extracted from raji cells. in vitro conversion of nuclei to ebna-positively was sensitive to dnase but not to rnase treatment. this suggests that nuclear dna is a specific target substance to which ebv-cf antigen binds. if nuclei were fix ...197882540
somatic cell hybrids between human lymphoma cell lines. v. idurd inducibility and p3hr-1 superinfectability of daudi/hela (dad) and daudi/p3hr-1 (dip-1) cell lines.we have studied two types of somatic cell hybrid with regard to expression of the epstein-barr virus (ebv) cycle and its regulation. the first, dip-1, a hybrid formed between two human lymphoma ebv producers (daudi and p3hr-1), contained ebv dna, expressed the virus-determined nuclear antigen (ebna), andwas a producer of the ebv-associated antigens ea (early antigen) and vca (viral capsid antigen). the second, dad, a hybrid series of clones formed between daudi and a hela cell derivative (d98), ...197882545
studies on epstein-barr virus-related antigens. iii. purification of the virus-determined nuclear antigen (ebna) from non-producer raji cells.the purification of epstein-barr virus-determined nuclear antigen (ebna) was attempted on the basis of its biochemical and physicochemical properties and its immunological specificity, assayed by the indirect single radial immunodiffusion test and anti-complement immunofluorescence absorption test. when non-producer raji cell extract was subjected to 20--60% saturated ammonium sulfate fractionation, followed by dna-cellulose chromatography and immunoadsorbent chromatography, ebna was purified mo ...197882547
selective stimulation and differentiation of early antigens in lymphoblastoid cell lines producing epstein-barr-like viruses.comparisons of early antigens (ea) of ebv-related lymphotropic herpes virus of old world primates have proved difficult to accomplish because antigen levels are low in infected cells and the ratio of virus capsid antigen to ea is generally high. to overcome these difficulties, we have developed a procedure which combines antigen stimulation with inhibition of late virus functions and results in the selective stimulation of ea. using this procedure, we have examined the ea of ebv, herpesvirus pap ...197882609
the association between undifferentiated nasopharyngeal carcinoma and epstein-barr virus shown by correlated nucleic acid hybridization and histopathological studies.all of 51 undifferentiated carcinomas of the nasopharynx were ebv-dna-positive and, in the few tests performed, ebna-positive as well. most of these patients also had high anti-ebv [vca and ea (d)] antibody titres. of four patients that had somewhat differentiated npcs, two were ebv-dna-positive with a corresponding serological picture, whereas two were negative with an ebv serology comparable to that of healthy donors. of seven lymphomas localized in the nasopharynx, one was ebv-dna-positive an ...197883285
biology and virology of the human malignant lymphomas: 1st milford d. schulz lecture.permanent cell lines have been established from twelve diffuse histiocytic lymphomas (su-dhl-1 to -12), three american burkitt's lymphomas (su-amb-1 to -3), two acute lymphoblastic leukemias (su-all-1 and -2), and three diffuse undifferentiated lymphomas (su-dul-1, -2, and -3). the cultured cells displayed neoplastic characteristics, as manifested by heterotransplantability in congenitally athymic nude mice and by the presence of cytogenetic abnormalities in early passage generations. functional ...197983902
characterization of a leukemic cell line of the pre-b phenotype. 197983966
infection with e.b. virus and rheumatoid arthritis. 197984110
the comparison between normal and epstein-barr virus-transformed homozygous typing cells and their use for mlc, plt and serological detection of human ia-type alloantigens.normal human lymphocytes isolated from freshly drawn blood were repeatedly stimulated (up to five times) in mixed lymphocyte cultures (mlc) with five different epstein-barr virus (ebv)-transformed homozygous typing cells (htc-lcl). each time, the same wdw-specificity was used for the restimulation. the first and the second stimulations resulted in an 2.4- to 3.1-fold increase of the original responder cell number. but after the third, fourth and fifth stimulation decreasing amounts of specifical ...197984412
transmission of non-a non-b hepatitis to chimpanzees by factor-ix concentrates after fatal complications in patients with chronic liver disease.6 cases of non-a non-b hepatitis which followed administration of four different batches of concentrates of coagulation factor ix from commercial and non-commercial sources are described. of 17 patients who received the concentrate on account of chronic liver disease, 4 developed hepatitis, and in 3 of these the illness proved fatal. the incubation periods ranged from 42 to 103 days (mean 65 days). 3 chimpanzees were inoculated with concentrate from the same batch used on the above patients, a f ...197985107
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