| [classification and virus expression of primary cerebral lymphomas]. | fourty-three primary cerebral lymphomas (pcl) were histologically classified and examined for genome expression of epstein barr virus (ebv) and human herpes virus 6 (hhv6) using dot blotting, polymerase chain reaction, and southern blotting. only 20 tumors (16 high grade and 4 low grade lymphomas) could be suitably placed into a category of the updated kiel classification, whereas the non-classified 23 tumors were highly malignant b-lymphomas and referred to as small-cell (sc) or large-cell (lc) ... | 1992 | 1283260 |
| detection of herpesvirus dna in the large intestine of patients with ulcerative colitis and crohn's disease using the nested polymerase chain reaction. | the prevalence of herpesvirus dna was examined in inflammatory bowel disease tissue. dna was extracted from resection and biopsy specimens of the large intestine from patients with ulcerative colitis (n = 21), patients with crohn's disease (n = 29), and patients with noninflammatory bowel disease (controls) (n = 21). the nested polymerase chain reaction was used to detect viral dna using primer pairs specific for either cytomegalovirus (cmv), herpes simplex virus 1 (hsv1), human herpesvirus 6 (h ... | 1992 | 1287131 |
| a chronic illness characterized by fatigue, neurologic and immunologic disorders, and active human herpesvirus type 6 infection. | to conduct neurologic, immunologic, and virologic studies in patients with a chronic debilitating illness of acute onset. | 1992 | 1309285 |
| serology versus clinical signs or symptoms and main laboratory findings in the diagnosis of exanthema subitum (roseola infantum). | twenty-five patients with clinical exanthema subitum (roseola infantum) were enrolled into a study, where acute-phase and convalescent sera were examined for antibodies to human herpesvirus 6 (hhv-6), several other viruses, and other microbes. in addition, an acute-phase fecal specimen was examined for viruses by electron microscopy. confirmative or suggestive serologic evidence for recent hhv-6 infection was obtained in 23 (92%) cases. conversion to hhv-6 was found in 19 (76%), a diagnostic (gr ... | 1992 | 1309393 |
| human herpesvirus-6: tumorigenicity and tumor infiltrating lymphocytes. | we previously reported that human herpes-virus-6 (hhv-6) genome (strain gs) and a cloned subfragment (pzvh14) transfected nih 3t3 cells, induced foci of transformation with a frequency significantly above the background level. the transformed cells produced tumors in nude mice and immunocompetent (swiss) mice. in the current study, nude mice tumors were passed into swiss mice and more aggressive tumors (g-2ts and 14-2ts derived from hhv-6 genome and pzvh14 dna, respectively) were produced. 14-2t ... | 1992 | 1309683 |
| identification and characterization of a human herpesvirus 6 gene segment that trans activates the human immunodeficiency virus type 1 promoter. | human herpesvirus 6 (hhv-6) is a lymphotropic herpesvirus, and in vitro, hhv-6 can productively infect many of the same cell types as can human immunodeficiency virus (hiv). coinfection by both viruses in vitro can lead to both activation of the hiv promoter and acceleration of cytopathic effects. we have previously demonstrated that a large, 22.25-kb cloned hhv-6 fragment, pzvb70, can trans activate hiv promoter expression in vitro. in this study, we show that the pzvb70 fragment can trans acti ... | 1992 | 1310766 |
| induction of host cell protein synthesis by human herpesvirus 6. | we observed an increase in host cell protein synthesis in human cord blood lymphocytes (cbl) infected with human herpesvirus 6 relative to uninfected cultures. the magnitude of this effect could not be explained by a smaller decrease in cell number in the infected cultures. the induction of host cell protein synthesis by hhv-6 does not appear to be mediated by a stable soluble factor present in the infected cell culture supernatant. when cbl were infected with virus that had been exposed to ultr ... | 1992 | 1311135 |
| analysis of human herpesvirus 6 glycoproteins recognized by monoclonal antibody ohv1. | a virus-specific glycoprotein (gp) from human herpes-virus 6 (hhv-6) was studied using the anti-hhv-6 monoclonal antibody ohv1. immunoprecipitation with extracts from infected cells revealed that the antibody recognized four glycosylated proteins (gps) with mrs of 106k, 102k, 65k and 63k under reducing conditions. however, only two gps, of 106k (gp106) and 102k, were detected under non-reducing conditions. pulse-chase experiments revealed that gp65 and gp63 were cleavage products of gp106 and gp ... | 1992 | 1311366 |
| [infection by human herpesvirus type 6: epidemiology, immunopathology and clinical implications]. | the human herpesvirus type 6 has been discovered recently and is the object of numerous investigations. even though, its morphology is very close to the cytomegalovirus, its epidemiologic, immunopathologic and clinic characteristics are similar to the epstein-barr virus. like the latter, hhv-6 persists latent in the host during all his live, frequently relapsed and is ubiquitous. exanthema subitum in children and mononucleosis-like syndrome in adults have been attributed to acute hhv-6 infection ... | 1992 | 1312248 |
| igg antibodies to human herpesvirus-6 (hhv-6) in italian people. | sera from a sample of healthy italian people were tested in an indirect immunofluorescence assay (ifa) for reactivity to a human herpesvirus-6 (hhv-6) strain called cv, isolated from a baby with exanthem subitum (portolani et al., 1990). seropositivity values of 83.78%, 92.68% and 63.64% were found in subjects aged 3 months-6 years, 6-18 years and over 18 years respectively. sera from cordal blood, sera from subjects with evidence of active infection by cytomegalovirus (cmv) or by epstein-barr v ... | 1992 | 1313531 |
| human herpesvirus-6. | | 1992 | 1313880 |
| human herpesvirus type 6: review. | human herpesvirus type 6 (hhv-6), a newly recognized human herpesvirus first described in 1986, is morphologically similar to other herpesviruses but is distinguishable from all of them by some unique in vitro biological effects, specific antigenic analysis, and patterns of endonuclease restriction digests of dna. in vitro hhv-6 exhibits tropism mainly for t lymphocytes, but it also infects other cells, including b lymphocytes, monocytes-macrophages, glial cells, and fibroblasts. because hhv-6 c ... | 1992 | 1314102 |
| antibodies to human herpes virus-6 and clinical course in patients with hodgkin's disease. | serial serum samples from 37 patients with hodgkin's disease (hd) and 39 healthy controls were studied for antibodies to human herpes virus-6 (hhv-6) using elisa and indirect immunofluorescent antibody (ifa) tests and to the epstein-barr virus (ebv) using a radio-complement fixation assay. antibodies to hhv-6 in the pre-treatment sera from the hd patients were not significantly different from those of controls, but significant changes in titers related to clinical course were noted among the hd ... | 1992 | 1314231 |
| in vitro suppression of bone marrow progenitor cell differentiation by human herpesvirus 6 infection. | suppression of marrow function may be one of the most serious effects of human herpesvirus 6 (hhv-6) infection in marrow transplant patients. in this study, normal bone marrow mononuclear cells were infected in vitro with hhv-6, and a methylcellulose-based colony formation assay was used to evaluate the impact of the infection on marrow cell differentiation and proliferation. results demonstrated that the outgrowth of colony-forming units of granulocyte and macrophage lineages (cfu-gm) was decre ... | 1992 | 1314872 |
| primary human herpesvirus 6 infection in young children. | human herpesvirus 6 (hhv-6) is a recently discovered virus that, on the basis of serologic evidence, appears to infect most children by the age of three years. however, the clinical manifestations of primary hhv-6 infection have not been well defined. | 1992 | 1315416 |
| human herpesvirus 6: the virus and the search for its role as a human pathogen. | | 1992 | 1315478 |
| human herpesvirus-6 dna in cerebrospinal fluid of a child with exanthem subitum and meningoencephalitis. | the involvement of human herpesvirus-6 (hhv-6) and herpes simplex virus infections was evaluated virologically and serologically in a 13-month-old girl with meningoencephalitic illness occurring in the pre-eruptive stage of exanthem subitum. an isolation of hhv-6 from blood and seroconversion to the virus confirmed the primary infection with the virus. hhv-6 gene sequences were detected in cerebrospinal fluid of acute stage of the disease by polymerase chain reaction. there was no evidence of he ... | 1992 | 1315948 |
| primary human herpesvirus 6 infections in children: a prospective serologic study. | | 1992 | 1316414 |
| geographic/ethnic differences in human herpesvirus-6 antibody patterns. | serum samples from healthy adults in four geographic/ethnic groups (ghanaian blacks, malaysian chinese, malaysian indians and united states caucasians) were tested under code for antibodies to human herpesvirus-6 (hhv-6). the prevalence and titer of hhv-6 antibody in the healthy ghanaians were significantly higher than in the malaysian chinese; united states caucasians and malaysian indians had intermediate prevalence and titer of antibodies. thus far, no specific differences in hhv-6-associated ... | 1992 | 1316534 |
| ["new" infectious diseases]. | | 1992 | 1317332 |
| fatal disseminated infection with human herpesvirus-6. | a 13-month-old immunocompetent girl had fever, rash, and multisystem disease, and she eventually died of cardiac failure. autopsy revealed intracellular viral inclusions of the herpesvirus group, with results of in situ hybridization positive for human herpesvirus-6. this is apparently the first case of fatal disseminated herpesvirus-6 infection. | 1992 | 1317420 |
| transformation of large granular lymphocytic leukemia during the course of a reactivated human herpesvirus-6 infection. | a patient with cd3+ large granular lymphocytic (lgl) leukemia developed transformation (tf). the phenotype of the leukemic cells was cd3+, cd4+ and cd8-. the leukemic cell count increased rapidly; the cells became large and the nuclear outline, which had been reniform, became lobulated. anti-htlv-1 and anti-hiv antibodies were negative in the serum of the patient and no htlv-1 specific sequences were detected in the cdna of the leukemic cells by polymerase chain reaction (pcr). comparison of the ... | 1992 | 1317489 |
| [human herpes virus 6. certainties and uncertainties]. | | 1992 | 1317960 |
| lymphadenopathy in connection with human herpes virus type 6 (hhv-6) infection. | two siblings and their mother developed afebrile generalized lymphadenopathy. the lymph nodes were movable and painless. during the course of the illness, the mother and one child developed an uncharacteristic rash. increased titers of human herpes virus type 6 (hhv-6) antibodies were found in all three family members and in an unrelated patient with lymphadenitis colli. the enlarged lymph nodes decreased in size within several weeks. we speculate these symptoms to be caused by an infection with ... | 1992 | 1318124 |
| human herpesvirus 6 in non-aids related hodgkin's and non-hodgkin's lymphomas. | | 1992 | 1318476 |
| [3-day fever: agreement between clinical diagnosis and seroconversion of type 6 human herpesvirus (hhv-6)]. | the aim of this study was to investigate the correlation between clinical diagnosis of exanthema subitum and seroconversion of herpes virus type 6 (hhv-6). 90 children with fever of unknown origin were investigated by 11 pediatricians in the canton of zürich, switzerland, between october 1989 and june 1990. antibodies against hhv-6 were determined at the first consultation and 2-3 weeks later. history and clinical findings were investigated and the clinical probability of the diagnosis exanthema ... | 1992 | 1318578 |
| human herpesvirus-6 infection and renal transplantation. | | 1992 | 1318588 |
| modulatory effects of epstein-barr, herpes simplex, and human herpes-6 viral infections and coinfections on cytokine synthesis. a comparative study. | herpesviruses such as ebv, hsv, and human herpes virus-6 (hhv-6) have a marked tropism for cells of the immune system and therefore infection by these viruses may result in alterations of immune functions, leading at times to a state of immunosuppression. we report the results of a comparative study in which we found that ebv, hsv-1, and hhv-6 act differentially on the immune system with regard to their effect on the synthesis of il-1 beta, il-6, and tnf-alpha, i.e., three immunoregulatory cytok ... | 1992 | 1318897 |
| replication of human herpesvirus 6 in epithelial cells in vitro. | mink lung epithelial (nbl-7) cells were shown to be permissive for human herpesvirus 6 (hhv-6) by four independent methods of analysis: detection of infectious virus, viral antigens, viral dna sequences, and herpesvirus particles. infection was serially passaged, with minimal cytopathology, for several months demonstrating for the first time that a cell of epithelial origin can support hhv-6 replication. | 1992 | 1318910 |
| evaluation of variables in immunofluorescence procedures for the detection of antibodies against human herpesvirus 6 (hhv-6). | ten human sera were used to study different parameters, namely, methods of smear preparation and fixation, and age of infected hsb-2 cells in order to optimize indirect immunofluorescence assay (ifa) and anticomplement immunofluorescence (acif) procedures to measure antibody levels against hhv-6. results showed a greater sensitivity of rapid smear drying and methanol fixation over conventional acetone smear preparation. cells harvested 6 days after infection and fixed with methanol exhibited a s ... | 1992 | 1319300 |
| [what is the relation of human herpesvirus 6 to chronic fatigue syndrome?]. | | 1992 | 1319541 |
| psychosocial modulation of antibody to epstein-barr viral capsid antigen and human herpesvirus type-6 in hiv-1-infected and at-risk gay men. | we investigated the effects of two behavioral interventions--aerobic exercise and cognitive behavioral stress management (cbsm)--on epstein-barr virus viral capsid antigen (ebv-vca) and human herpesvirus type-6 (hhv-6) antibody modulation in 65 asymptomatic gay men measured at several time points in the 5 weeks preceding and following notification of their human immunodeficiency virus-type 1 (hiv-1) serostatus. after accounting for potential immunomodulatory confounds, we found that hiv-1 seropo ... | 1992 | 1320279 |
| newest human herpesvirus (hhv-6) in the guillain-barré syndrome and other neurological diseases. | to investigate the presence of human herpesvirus-6 (hhv-6) in patients affected by guillain-barré syndrome (gbs) and by other neurological diseases (ond), we examined by indirect immunofluorescence analysis (ifa) the sera and cerebrospinal fluid (csf) from 28 gbs and 63 ond. moreover, we tested 150 blood donors (bd) to appreciate the diffusion of hhv-6 infection in the italian adult healthy population. we found a significantly higher titre of antibody to hhv-6 in the gbs patients compared with o ... | 1992 | 1320318 |
| anti-hhv-6 antibodies in normal population and in cancer patients in india. | the prevalence and titre of igg antibodies to human herpesvirus type 6 (hhv-6) were assayed in the serum samples from normal subjects and patients with hodgkin's lymphoma (hl), non-hodgkin's lymphoma (nhl), acute lymphoblastic leukaemia (all) and oral cancer (oc) using immunofluorescence and immunoperoxidase techniques. this forms the first study on the sero-prevalence and titre of antibodies to hhv-6 in india. there was no considerable difference in the prevalence (76%) and titre (10-160) of th ... | 1992 | 1320669 |
| the right end of the unique region of the genome of human herpesvirus 6 u1102 contains a candidate immediate early gene enhancer and a homologue of the human cytomegalovirus us22 gene family. | the nucleotide sequence of a 12 kbp hindiii fragment (hindiii c) from the right end of the unique component of the genome of human herpesvirus 6 (hhv-6) (strain u1102) was determined. the sequence has a mean g + c content of 42% and contains approximately 28 copies of a tandemly repeated 104 to 107 bp element, which, with a single exception, contain a cleavage site for kpni (the kpni repeats). each of these elements contains potential binding sites for transcription factors nf-kappa b and ap2. t ... | 1992 | 1321205 |
| identification of homologues to the human cytomegalovirus us22 gene family in human herpesvirus 6. | the sequence of 10079 bp corresponding to the overlapping sali h and smai g restriction fragments of the genome of human herpesvirus 6 (hhv-6) strain u1102 was determined. the sequence contains six complete open reading frames (orfs) and two incomplete orfs located at the 5' and 3' ends of the sali h and smai g fragments respectively. seven of these orfs have recognizable homologues only in the beta-herpesvirus human cytomegalovirus (hcmv), no obvious counterparts being detectable in the genomes ... | 1992 | 1321206 |
| experimental infection of cynomolgus and african green monkeys with human herpesvirus 6. | cynomolgus and african green monkeys were inoculated with human herpesvirus 6 (hhv-6). an antibody response was first observed 10 days and 5 days after inoculation of cynomolgus monkeys and african green monkeys, respectively, and was detectable for the duration of the experiment (33 days). hhv-6 dna was first detected by the polymerase chain reaction in mononuclear cells of one cynomolgus monkey and one african green monkey 10 days after virus inoculation, and in a total of three of four cynomo ... | 1992 | 1321207 |
| identification of the glycoprotein h gene of murine cytomegalovirus. | partial sequencing of the hindiii c fragment of murine cytomegalovirus (mcmv) revealed an open reading frame of 2172 nucleotides in length encoding a 724 amino acid protein with a predicted mr of 80.4k. analysis of the predicted amino acid sequence revealed homology with glycoprotein h (gh) from a number of other herpesviruses. mcmv gh showed strongest amino acid identity with human (h) cmv and human herpesvirus 6 gh, and less identity with the gh protein sequences of epstein-barr virus, varicel ... | 1992 | 1321219 |
| detection of human herpesvirus 6 in tissues involved by sinus histiocytosis with massive lymphadenopathy (rosai-dorfman disease). | after preliminary serologic data demonstrated elevated antibody titers to human herpesvirus (hhv) 6 in patients with sinus histiocytosis with massive lymphadenopathy (shml) or rosai-dorfman disease, tissues were examined from 9 patients with classical shml to search for evidence of hhv-6 infection. involved tissues from 7 of the 9 patients had detectable hhv-6 by in situ hybridization: tissue from 1 had detectable epstein-barr virus genome but no hhv-6 and tissue from another had no detectable h ... | 1992 | 1321861 |
| the chronic fatigue syndrome controversy. | | 1992 | 1322077 |
| human herpesvirus-6: clinical implications of a recently discovered, ubiquitous agent. | | 1992 | 1322455 |
| absence of antibodies to human herpesvirus-6 in patients with slowly-progressive human immunodeficiency virus type 1 infection. | to evaluate a possible role for human herpesvirus-type 6 (hhv-6) coinfection as a co-factor in the progression of hiv-1 disease, we investigated the prevalence of seropositivity for hhv-6 in a cohort of hiv-1 infected patients. these patients were retrospectively divided into two groups according to the decline of cd4+ t cells during the follow up: 11 were classified as rapid decliners (less than 400 cd4+/cmm within 1 year), and 38 as slow decliners (greater than 400 cd4+/cmm after at least 4 ye ... | 1992 | 1322823 |
| evidence for putative immediate early antigens in human herpesvirus 6-infected cells. | human herpesvirus 6 (hhv-6) induced nuclear antigens in cells as early as 3 h after infection. these nuclear antigens were induced by all three strains of hhv-6 tested, and their de novo synthesis required the function(s) of the intact viral genome. their appearance was not affected by 2,2'-anhydro(1-beta-d-arabinofuranosyl)cytosine, but was completely inhibited by cycloheximide. however, the nuclear antigens did appear if cycloheximide was replaced with actinomycin d. thus, the nuclear antigens ... | 1992 | 1322964 |
| clinical, epidemiologic, and virologic studies in four clusters of the chronic fatigue syndrome. | the purpose of this study is to provide a case definition of chronic fatigue syndrome in an outbreak occurring in the nevada-california region to evaluate candidate etiologic agents and observe the natural history of the illness. | 1992 | 1323246 |
| characterization of human herpesvirus 6 strains isolated from patients with exanthem subitum with or without cutaneous rash. | | 1992 | 1323627 |
| [seroepidemiologic study of human herpesvirus-6 in intravenous drug addicts, with and without infection caused by human immunodeficiency virus type 1]. | the human herpesvirus-6 (hhv-6) may be a cofactor of infection by the human immunodeficiency virus type 1 (hiv-1). however, there are discrepancies with respect to the possible epidemiological relation between both viruses. the aim of the present study was to study the prevalence of infection by the hhv-6 in intravenous drug addicts (ivda) with and without hiv-1 infection. | 1992 | 1324385 |
| recent problems in viral infections of the nervous system. | | 1992 | 1324559 |
| human herpesvirus 6 (hhv-6) infection in the central nervous system. | human herpesvirus 6 (hhv 6) was isolated from patients with exanthema subitum (es) with a high frequency, and it is now believed that this virus causes es as a primary infection in childhood. hhv 6 infection is highly prevalent in early childhood and this virus may infect infants through the saliva mainly from mother to child. hhv 6 has a tropism to cd4+ cells and destroys cells in vitro. although children recover from es without any sequelae, neurological symptoms associated with exanthema subi ... | 1992 | 1324560 |
| igm neutralizing antibody responses to human herpesvirus-6 in patients with exanthem subitum or organ transplantation. | the assay for detecting igm neutralizing (nt) antibody activity to human herpesvirus-6 (hhv-6) was developed by using pretreatment of blood sample with staphylococcal protein a. the activity was mostly present in igm fractions of serum but not in iga fractions separated by ultracentrifugation. the assay was used for seroepidemiological studies for hhv-6 infection. in primary hhv-6 infection, igm nt antibodies appeared 5 to 7 days after onset of exanthem subitum, reached maximum titers at 2 to 3 ... | 1992 | 1325026 |
| human herpesvirus 6. | | 1992 | 1326076 |
| herpesvirus 6 infection in young children. | | 1992 | 1326080 |
| in situ detection of human herpesvirus 6 in retinitis associated with acquired immunodeficiency syndrome. | | 1992 | 1326231 |
| frequency of dual infections of corneas with hiv-1 and hhv-6. | in the current study, 35 pairs of corneas from asymptomatic carriers of hiv-1 and ten pairs from aids patients were analyzed for the presence of hiv-1 and hhv-6. the tissues were evaluated for viral antigens, transcripts, dna sequences and intact and infectious virus. three corneas from two asymptomatic carriers of hiv-1 and three corneas from two aids patients were culture positive for hiv-1. one of the three hiv-1 positive corneas from an asymptomatic hiv-1 carrier also was culture positive fo ... | 1992 | 1326427 |
| evidence of human herpesvirus 6 in sjögren syndrome and sarcoidosis. | | 1992 | 1326434 |
| transcriptional activity of hiv-1 and hhv-6 in retinal lesions from aids patients. | this study determined the frequency of multiple viral (hiv-1, hhv-6, and cmv) infections in 26 retinas from 16 aids patients. of the 12 retinas of 26 that tested positive for hiv-1 dna sequences, seven also were positive for hhv-6 dna sequences. four of these seven retinas were culture positive for hiv-1 and two of the four contained cmv dna sequences and antigens. using rna probes, hiv-1 and hhv-6 transcriptional activity was demonstrated in two of the four hiv-1 culture positive retinas. these ... | 1992 | 1326493 |
| [neuraxitis in critical exanthema: description of a case]. | in this work the authors describe the case of a 16-month-old child, who presented neuraxitis infection of cerebellum, during exanthem subitum. from the present etiological considerations about exanthem subitum, referable to human herpes virus type 6, the authors stress the usefulness of executing the dosage of anti-hhv6 antibodies in all the cases of exanthem subitum and in all febrile convulsions, because it is possible to have atypical clinic forms of vi disease, without exanthem. | 1992 | 1326753 |
| phenotypic and genetic polymorphisms among human herpesvirus-6 isolates from north american infants. | fifteen human herpesvirus-6 (hhv-6) isolates from north american infants with primary infection manifest as febrile or roseola (exanthem subitum) like illnesses were characterized phenotypically on the basis of their in vitro growth in continuous t-cell lines and primary human mononuclear cells and by their reactivity with monoclonal antibodies. all isolates replicated efficiently in primary human cord blood mononuclear cells, but five distinct patterns of viral replication in human cells lines ... | 1992 | 1326815 |
| viral infections in dentistry. | viral diseases of relevance to dentistry have recently been reviewed with respect to human immunodeficiency virus disease, other immunocompromised persons, oral malignancies, infection control, and antiviral therapy. this review discusses the most recent advances in the understanding of aspects of human immunodeficiency virus relevant to dentistry and relevant aspects of the herpesviruses, human papillomaviruses, hepatitis viruses, and other viruses. further detail is available in other recent r ... | 1992 | 1327293 |
| epidemiological and biological study of acquired immunodeficiency syndrome-related lymphoma in the county of los angeles: preliminary results. | a population-based case control study of intermediate- and high-grade lymphoma in the county of los angeles, ca, was initiated in 1989. human immunodeficiency virus (hiv)-positive lymphoma patients are compared to hiv-negative lymphoma patients, to hiv-positive controls with acquired immunodeficiency syndrome but without lymphoma, and to hiv-positive asymptomatic individuals. the hiv-negative lymphoma cases are compared to neighborhood controls, who are matched in terms of age, sex, race/ethnici ... | 1992 | 1327509 |
| several groups among human herpesvirus 6 strains can be distinguished by southern blotting and polymerase chain reaction. | | 1992 | 1328291 |
| human herpes virus 6 and endogenous biotin in salivary glands. | to detect the presence of human herpes virus 6 (hhv6) and endogenous biotin in paraffin wax embedded and frozen salivary glands. | 1992 | 1328329 |
| acute encephalopathy following exanthem subitum caused by human herpesvirus-6. | | 1992 | 1328421 |
| antigenic relationships among human herpesvirus-6 isolates. | human herpesvirus 6 (hhv-6) prototype isolate gs is a newly identified lymphotropic herpesvirus and several subsequent herpes isolates were recognized as hhv-6 by their hybridization to a hhv-6(gs) dna probe pzvh14. dna restriction analysis and in vitro tropism studies show that hhv-6 isolates can be divided into two groups, designated group a and group b. antigenic relationships among 15 hhv-6 isolates belonging to these two groups were examined using rabbit antibodies against hhv-6(gs) infecte ... | 1992 | 1328500 |
| detection of human herpesvirus-6 dna by polymerase chain reaction in serum or plasma. | human herpesvirus-6 (hhv-6) is a newly identified human pathogen. currently clinicians rely mainly on blood lymphocyte culture and serological tests to diagnose hhv-6 infection. the polymerase chain reaction (pcr) was carried out on the plasma or sera of patients to determine the value of pcr in the diagnosis of hhv-6 infection. a total of 30 patients entered the study; 10 were experiencing acute hhv-6 infections and 20 were healthy and served as controls. hhv-6 dna was detected by pcr in the se ... | 1992 | 1328512 |
| [complications of acute respiratory tract infection (pneumonia, sinusitis) in young children associated with acute hhv 6 infection]. | the human herpesvirus-6 (hhv-6) causes exanthema subitum ("e.s."). "e.s." is characterized by fever, exanthem (rash), in many cases gastroenteritis, occasionally cerebral convulsions (but more frequently general cerebral irritability) and enlargement of all lymph nodes; usually there are mild catarrhal respiratory symptoms of the upper airways (ard). so-called "complications" of an ard (pneumonia, acute purulent otitis media, acute sinusitis) due to bacterial infections are very unusual as seque ... | 1992 | 1328758 |
| antiviral activity of a bile pigment, biliverdin, against human herpesvirus 6 (hhv-6) in vitro. | biliverdin (bv), a bile pigment, was examined for its antiviral activity against human herpesvirus-6 (hhv-6) in vitro. bv (10 micrograms/ml) markedly inhibited hhv-6 replication in mt-4 cells when the cells were treated during a virus adsorption period. its antiviral effect was weakened when cells were treated after adsorption. treatment of cells with bv (40 micrograms/ml) 3 hr after virus infection had no inhibitory effect on virus replication. virus replication was also significantly inhibited ... | 1992 | 1328826 |
| primary human herpesvirus-6 infection (exanthem subitum) in the newborn. | | 1992 | 1329004 |
| [infection by human herpesvirus type 6 (hhv-6) and renal transplantation]. | human herpes virus type 6 (hhv-6) infection was serologically investigated in renal transplant recipients. before transplantation, 75.5% of patients was seropositive for hhv-6 and no correlation with age, sex and time on dialysis was found. during the first month after transplantation 66% of patients showed a variation in serological status against hhv-6 (seroconversion or fourfold increase of antibody titer). all patients who seroconverted had received the kidney from a hhv-6 seropositive donor ... | 1992 | 1329241 |
| characterization of human herpesvirus-6(u1102) and (gs) gp112 and identification of the z29-specified homolog. | monoclonal antibody 2d10 (mab 2d10) raised toward human herpesvirus-6(u1102) [hhv6(u1102)] immunoprecipitated three glycosylated peptides, m(r) 112,000, 64,000, and 58,000, designated as gp112 from u1102-infected lymphocytes. pulse-chase experiments suggest that the m(r) 64,000 and 58,000 polypeptides are very likely generated by post-translational cleavage of the m(r) 112,000 polypeptide. mab 2d10 neutralized virion infectivity in the presence of complement, suggesting that gp112 is located in ... | 1992 | 1329335 |
| homology of the envelope glycoprotein b of human herpesvirus-6 and cytomegalovirus. | the envelope glycoprotein b (gb) coding sequences of two strains of human herpesvirus-6 (hhv6 gs and z29) were determined by sequencing a 2.5-kb open reading frame adjacent to the dna polymerase sequence. the deduced primary translation product is 830 amino acids in length and is 96% conserved between the two divergent strains with no localized hypervariability noted. it contains the expected signal and transmembrane sequence motifs as well as a putative site of protease cleavage. there was 39% ... | 1992 | 1329336 |
| hhv-6 inhibition by two polar compounds. | dimethyl sulphoxide and dimethyl formamide, two polar compounds and powerful cell differentiation inducers, inhibit hhv-6 infection when added to hhv-6-infected hsb2 cultures. this was established by a delay in the time-course of infection and in the development of virus-induced cytopathic effects. furthermore, viral titration of supernatants showed a significant reduction (3 log10) of the number of infectious particles. electron microscopy confirmed that viable cells and extracellular virions w ... | 1992 | 1329644 |
| herpesvirus serology, aberrant specific immunoglobulin g2 and g3 subclass patterns and gm allotypes in individuals with low levels of igg3. | one objective of this study was to determine whether igg3-deficient individuals have an increased frequency of reactivated herpesvirus infections. serum titres to epstein-barr virus (ebv) and human herpesvirus-6 were examined in 10 healthy and in 10 symptomatic persons with serum igg3 < 0.1 g/l. atypical titres were found in 16% of the igg3-deficient individuals. reactivations of these viruses thus do not seem common in igg3 deficiency. antigen-specific igg responses were also determined. a lowe ... | 1992 | 1330389 |
| comparison of three immunoassays for the detection of anti-hhv6. | sera from 96 blood donors were tested for antibody to human herpesvirus 6 by indirect immunofluorescence (if), circle immunoassay (cia) and competitive radioimmunoassay (ria). the correlation between the three assays was good but the cia and competitive ria were more sensitive for the detection of hhv6 antibody than indirect if. the crossreaction of hhv6 antibody with that to the other human herpesviruses was also studied in this blood donor group. no correlation was found between antibody to hu ... | 1992 | 1331142 |
| endonuclease analyses of dna of human herpesvirus-6 isolated from blood before and after bone marrow transplantation. | three strains of human herpesvirus-6 (hhv-6) were isolated from peripheral blood mononuclear cells of a leukemic child with the antibody of hhv-6 before and after bone marrow transplantation (bmt); two strains were obtained before bmt and one after bmt. the dna extracted from the three isolates was analyzed by six different restriction endonucleases. the cleavage profiles of two strains obtained before bmt were different, but the third strain isolated after bmt was identical with one of the two, ... | 1992 | 1331312 |
| antibodies to human herpes virus-6 in patients with acute lymphocytic leukemia. | human herpesvirus-6 (hhv-6), a ubiquitous virus that causes exanthem subitum and occasional cases of infectious mononucleosis, hepatitis and other viral syndromes, has also been associated with acute lymphocytic leukemia (all) in children. to further investigate this association, we obtained sera from 50 patients with all and 50 age-sex matched controls. antibodies to hhv-6 were determined using elisa and indirect immunofluorescent antibody (ifa) tests. no significant difference between antibody ... | 1992 | 1331626 |
| human herpesvirus 7 is a t-lymphotropic virus and is related to, but significantly different from, human herpesvirus 6 and human cytomegalovirus. | an independent strain (ji) of human herpesvirus 7 (hhv-7) was isolated from a patient with chronic fatigue syndrome (cfs). no significant association could be established by seroepidemiology between hhv-7 and cfs. hhv-7 is a t-lymphotropic virus, infecting cd4+ and cd8+ primary lymphocytes. hhv-7 can also infect sup-t1, an immature t-cell line, with variable success. southern blot analysis with dna probes scanning 58.8% of the human herpesvirus 6 (hhv-6) genome and hybridizing to all hhv-6 strai ... | 1992 | 1332051 |
| viral pneumonia in the immunocompromised adult with neoplastic disease: the role of common community respiratory viruses. | until recently, the viruses most often identified in lower respiratory tract infections among immunocompromised adults with neoplastic disease have been the herpesviruses (cytomegalovirus, herpes simplex virus, varicella-zoster virus, human herpesvirus-6) and adenovirus. these viral pneumonias occur with greater frequency and/or severity among these adults than among immunocompetent adults. during the last decade, mounting evidence has suggested that the rna viruses that commonly cause lower res ... | 1992 | 1332171 |
| a prospective study of human herpesvirus-6 infection in renal transplantation. | sixty-five kidney transplant recipients and their (22 living related and 43 cadaveric) donors were studied prospectively to determine the relationship between kidney transplantation and human herpesvirus-6 (hhv-6) infection. the virus isolation from peripheral blood and other tissues and sequential determination of neutralizing antibodies to hhv-6 were performed during 3 months following the transplantation. all of the donors and their recipients examined had neutralizing antibodies to hhv-6 at ... | 1992 | 1332225 |
| cytomegalovirus meningoencephalitis in healthy adults with coincident infection by human herpesvirus type 6. | | 1992 | 1332667 |
| coamplified positive control detects inhibition of polymerase chain reactions. | polymerase chain reactions (pcr) may fail to react because the substrate dna is absent (true negative) or because of inhibition of specific amplification (false negative). the use of positive controls can increase confidence in negative pcr results by ruling out failure due to inhibition as a cause of the lack of amplification products. this report describes the construction and application of coamplified positive controls for herpes simplex virus and human herpesvirus 6 amplifications. herpes s ... | 1992 | 1333488 |
| infectivity determinants encoded in a conserved gene block of human herpesvirus-6. | the nucleotide sequence was determined for a 9.3 kb bamhi dna fragment derived from a cosmid clone (lorist 6) library of the 160 kb human herpesvirus-6 (hhv-6) strain u1102 genome. analysis of the sequence showed two different sources for the dna; 8.0 kb was derived from hhv-6, while 1.3 kb was derived from the right repeat of transposon tn10, the insertion sequence (is) element is10r. the is element sequence is shown to be derived from the host bacteria of the plasmid. the hhv-6 sequence repres ... | 1992 | 1333836 |
| viral involvement in hodgkin's disease. | the etiology of hodgkin's disease (hd) is unknown, but a growing body of evidence suggests that the epstein-barr virus (ebv) plays a role in a proportion of cases. clonal ebv genomes have been detected in affected tissues, and ebv has been localized to reed-sternberg (rs) cells, the putative malignant cells in hd. ebv latent genes, including the eber rnas and the latent membrane protein, lmp-1, are expressed by rs cells. these data suggest that ebv is playing a role in the pathogenesis of hd; ho ... | 1992 | 1334112 |
| isolation of human herpesvirus-6 from an infant with kawasaki disease. | | 1992 | 1334835 |
| activation of human herpesvirus-6 in children with acute measles. | virological and serological studies were carried out prospectively to evaluate the possible activation of human herpesvirus-6 (hhv-6) in 50 infants and children with acute measles by isolation of hhv-6 from peripheral blood and by determining neutralizing antibodies to the virus. all but 5 patients (90%) were seropositive to hhv-6 in the acute stage of measles and 18 (40%) had a significant increase in hhv-6 antibody titers thereafter, whereas only 2 of 27 patients who were initially seropositiv ... | 1992 | 1335483 |
| hhv-6 and hhv-7 as exogenous agents in human lymphocytes. | human herpesviruses 6 and 7 (hhv-6 and hhv-7), the newly recognized lymphotropic members of the herpesvirus family, were isolated from peripheral blood mononuclear cells (pbmc) of healthy individuals. hhv-7 was recovered from pbmc after exposing the cells to conditions leading to t cell activation. no virus could be recovered from quiescent non-activated cultures, suggesting that the virus resided latently in these cells and that it could be induced from latency by t cell activation. hhv-6 could ... | 1992 | 1335933 |
| [infections with herpesvirus 6--really only "exanthema subitum"? part 1: more frequent disease pictures]. | the human herpes virus 6 (hhv6) is the causal agent of exanthem subitum (rose rash of infants). in addition, however, a wide range of other clinical manifestations are possible, the more common of these include: untypical exanthem or uncharacteristic rash (with and without fever), acute respiratory disease (ard) acute gastroenteritis and febrile convulsions (with and without exanthem). more uncommon, and in part unknown, clinical manifestations associated with hhv6 infection are discussed in par ... | 1992 | 1335952 |
| [infections with herpesvirus 6--really only "exanthema subitum"? part 2: rare or unknown disease pictures]. | the human herpes virus 6 (hhv 6) may induce not only the wellknown condition of exanthem subitum, but also a number of more common (cf. part 1) or rare, even previously unknown, clinical manifestations. part 2 of this paper deals with the more rarely observed manifestations. these include complications of ard (sinusitis, otitis media, bronchial pneumonia) hepatitis, encephalitis or pfeiffer's disease (mononucleosis-like syndrome). in individuals with a relevant disposition (genetic hla/dr type?) ... | 1992 | 1335953 |
| [the significance of determination of human herpesvirus-6 (hhv-6) antibody during pregnancy]. | hhv-6 which was detected in peripheral blood lymphocytes of lymphocytic patients is now said to be an agent causing exanthema subitum. we investigated the titer of hhv-6 antibody in 100 primigravida and 30 patients with spontaneous abortion by means of the indirect immunofluorescence method (if method). the positive incidence in which the titer of hhv-6 antibody was more than 10 times in 100 primigravida was 82%. that of hhv-6 igm antibody was 3% in those who had no complication during pregnancy ... | 1992 | 1336531 |
| fatal encephalitis/encephalopathy in primary human herpesvirus-6 infection. | an encephalitic illness with a fatal outcome occurred in a 9 month old girl with virologically confirmed exanthem subitum. human herpes-virus-6 (hhv-6) dna was found in the cerebrospinal fluid at the acute stage of the disease by the polymerase chain reaction, but the virus antigen was not detected in her brain tissue. this suggests that hhv-6-induced encephalitis/encephalopathy may be due to a non-infectious process. | 1992 | 1336954 |
| infection by human herpesvirus 6 (hhv-6) of human lymphoid t cells occurs through an endocytic pathway. | we have analyzed by immunoelectron microscopy the early events of binding and internalization of human herpesvirus 6 (hhv-6, strain gs) on a susceptible t-lymphoblastoid cell line, hsb-2. the virions bound to the cell surface at 4 degrees c were tightly associated with the plasma membrane. gold immunolabeling of the viral envelope proteins was strong and specific. warming at 37 degrees c for different times showed viral internalization through smooth surfaced pits and vesicles. fusion events of ... | 1992 | 1337259 |
| human herpesvirus-6 infection and bone marrow transplantation. | human herpesvirus-6 (hhv-6) is ubiquitous in the human population and causes exanthem subitum, a benign disease seen in infancy. however it also produces a wide spectrum of clinical manifestations including cases with a fatal outcome. the virus remains latent in several organs, including the kidneys, liver, lymph/nodes and salivary glands, after the primary infection and reactivates when immune function is impaired. reactivation of the virus occurred in a half of the bone marrow recipients 2 to ... | 1992 | 1337298 |
| prevalence of herpesvirus, human t-lymphotropic virus type 1, and treponemal infections in southeast asian refugees. | sera obtained for treponemal serology (vdrl) from 193 southeast asian refugees representing five ethnic groups seen in a primary care clinic were examined for antibodies to human t-lymphotropic virus type 1 (htlv-1), human herpes-virus-6 (hhv-6), epstein-barr virus (ebv), and cytomegalovirus (cmv). the seroprevalence was highest for ebv (99%), followed in decreasing order by cmv (95%), hhv-6 (26%), and htlv-1 (0.6%). the vdrl was positive in 15% of patients. the highest seroprevalence to hhv-6 w ... | 1992 | 1337548 |
| [chronic fatigue syndrome and virus infection: human herpesvirus 6 (hhv-6) infection]. | chronic fatigue syndrome (cfs) is newly-recognized disease characterized by chronic and debilitating fatigue. it has been suggested that viral infection may be involved in this syndrome from the results of clinical examination, including increased activity of 2',5'-synthetase in leukocytes of patients. the following viruses have been reported as etiologic agents of this disease. first, many studies have found elevated levels of igg to viral capsid antigen and early antigens to epstein-barr virus ... | 1992 | 1337558 |
| [viral infection and its causative role for chronic fatigue syndrome]. | patients with chronic fatigue syndrome (cfs), of unknown etiology, have been increasingly reported. this syndrome is characterized by debilitating fatigue, lymphadenopathy, and fever. herein, i focus on and review this syndrome from the view point of the causative role of viral infection. since the symptoms of cfs are similar to those of chronic infectious mononucleosis (cim) or chronic epstein-barr virus infection (cebv), the role of ebv has been intensively studied. the etiological relationshi ... | 1992 | 1337559 |
| [chronic fatigue syndrome--study of 51 cases treated at the second tokyo national hospital]. | fifty-one patients with chronic fatigue syndrome (cfs) were studied. tender points, which are a characteristic clinical feature of fibromyalgia, were found in all but two of the patients at 11.4 points (mean) per patient. igg antibody titers to eb virus viral capsid antigen were more elevated in the cfs patient group compared to that of the control (p < 0.0015). igg antibody titers to hhv-6 were not higher in the patient group. nk cell activity was not more decreased in the patient group, wherea ... | 1992 | 1337560 |
| [chronic fatigue syndrome, a case of high anti-hhv-6 antibody titer and one associated with primary hyperaldosteronism]. | two cases of chronic fatigue syndrome (cfs) were reported which were suggestive for the study of the etiology and a cure for cfs. case 1: a 31-year-old woman was admitted for chronic fatigue syndrome. examination revealed a high titer of anti hhv-6 antigen of x2560 and an increased percentage of suppressor t lymphocytes in the peripheral blood. hhv-6 was speculated to be reactivated and stimulating the immune system in cfs. case 2: a 46-year-old woman suffering from cfs had been in remission for ... | 1992 | 1337563 |
| immune surveillance, organophosphorus exposure, and lymphomagenesis. | prevalence of lymphoproliferative disorders is increased in populations with various chemical exposures, including organophosphorus compounds. lymphomas are also more common in individuals with a substantially decreased monocyte esterase activity. organophosphorus compounds inhibit esterases associated with monocytes, natural killer (nk) cells, lymphokine-activated killer (lak) cells, and cytotoxic t lymphocytes, and these inhibitory effects impair immune surveillance and cytotoxic functions med ... | 1992 | 1346889 |
| massive vancouver conference focuses on tragedy of world's dying children. | | 1992 | 1348205 |
| establishment and functional characterization of human herpesvirus 6-specific cd4+ human t-cell clones. | in order to clarify the protective immune responses against a newly identified herpesvirus, human herpesvirus 6 (hhv-6), we established hhv-6-specific human t-cell clones and examined their functional properties. five cd3+cd4+cd8- t-cell clones, which proliferated in response to stimulation with two different strains of hhv-6 in the presence of autologous antigen-presenting cells but not with herpes simplex virus type 1 or human cytomegalovirus, were established from peripheral blood lymphocytes ... | 1992 | 1348547 |
| human herpesvirus 7 is a constitutive inhabitant of adult human saliva. | we report the frequent isolation of human herpesvirus 7 from the saliva of healthy adults. virus isolates recovered from different individuals exhibited minimal restriction enzyme polymorphism, which was mostly confined to heterogeneous (het) sequences in the genome. dnas of isolates recovered from the same individual over a period of several months showed the same characteristic het fragments, indicating the stability of the het sequences upon virus replication and shedding in vivo. in contrast ... | 1992 | 1348548 |