| preparation of rabbit immune serum with neutralizing activity against a simian cytomegalovirus (sa6). | | 1969 | 4303878 |
| stringent regulation of human growth hormone expression in cultured murine c2c12 myoblasts by the e. coli lac repressor. | gene transfer techniques can be used to encode the production of a polypeptide product, such as human growth hormone (hgh), that is missing in an acquired or inherited disease state such as growth hormone deficiency. in one model system, engineered c2c12 myoblasts are injected intramuscularly into a mouse and subsequently secrete hgh into the circulation. in this regard, a gene-expression regulatory system that functions in myoblasts would be of interest. we demonstrate that the escherichia coli ... | 1995 | 7704337 |
| cytomegalovirus protein substrates are not cleaved by the herpes simplex virus type 1 proteinase. | the herpesvirus maturational proteinase, assemblin, is made as a precursor that undergoes at least two autoproteolytic cleavages--one in a sequence toward its carboxyl end, called the maturational (m) site, and one in a sequence toward its midpoint, called the release (r) site. the m- and r-site sequences are both well conserved among the herpesvirus proteinase homologs, suggesting that the proteinase of one herpesvirus might be able to cleave the substrates of another. to test this possibility, ... | 1995 | 7983728 |
| human cytomegalovirus proteinase: candidate glutamic acid identified as third member of putative active-site triad. | the human cytomegalovirus (hcmv) proteinase is synthesized as a 709-amino-acid precursor that undergoes at least three autoproteolytic cleavages. the mature proteinase, called assemblin, is one of the products of autoproteolysis and is composed of the first 256 amino acids of the precursor. hcmv assemblin and its homologs in other herpes group viruses contain five highly conserved domains (cd1 through cd5). an absolutely conserved serine in cd3 has been shown by site-directed mutagenesis of the ... | 1995 | 7769716 |
| a cytomegalovirus dna sequence containing tracts of tandemly repeated ca dinucleotides hybridizes to highly repetitive dispersed elements in mammalian cell genomes. | a single 880-base-pair region within the genome of simian cytomegalovirus strain colburn contains sequences that hybridize intensely with both human and mouse total genome dna probes. this sequence was also found in a second simian cytomegalovirus isolate and was retained in both plaque-purified virus subclones and in plasmid dna clones containing the sali p fragment. cleaved genomic dnas from several mammalian species all exhibited strong dispersed hybridization with the sali-p probes, and over ... | 1983 | 6312291 |
| natural and experimental simian cytomegalovirus infections at a primate center. | simian cytomegalovirus infections were studied in captive, naturally infected primates and in experimentally infected rhesus monkeys. neutralizing antibody to simian cytomegalovirus was prevalent in selected species of old world monkeys. naturally infected, rhesus monkeys shed virus in their urine during the entire two-year period of study. similarly, experimentally infected rhesus monkeys showed neutralizing antibody and viruria for more than two years. the indirect fluorescent antibody procedu ... | 1982 | 6306240 |
| comparison of simian and human cytomegalovirus reactivities in an enzyme-linked immunospecific assay: effect of antigen preparation on cross-reactive antigens. | simian cytomegalovirus was substituted for human cytomegalovirus in an enzyme-linked immunoassay. unlike the indirect immunofluorescence assay which demonstrates a two-way cross-reactivity, only one-way cross-reactivity was observed. altering the method of simian antigen preparation gave some insight other this different reactivity. | 1982 | 6288573 |
| comparison of monoclonal and polyclonal antibody for confirmation of cytomegalovirus isolates by fluorescent staining. | a fluorescein isothiocyanate-labeled monoclonal cytomegalovirus antibody reagent incorporating evans blue (syva/genetic systems, palo alto, calif.) was compared to a fluorescein isothiocyanate-labeled polyclonal antibody (dynatech diagnostics, inc., south windham, maine) for identification of cytomegalovirus in tissue culture. a total of 191 cultures, 86 positive and 105 negative for cytomegalovirus by cytopathic effect in wi38 cells, were stained in parallel and examined by fluorescent microsco ... | 1984 | 6088576 |
| differentiation of cross-reacting simian cytomegalovirus strains by late 19s rabbit neutralizing antibodies. | | 1969 | 4310839 |
| infection of vervet monkey bone marrow stromal cells with simian cytomegalovirus. | | 1987 | 3040871 |
| experimental congenital disease with simian cytomegalovirus in rhesus monkeys. | cytomegalovirus (cmv) infections occur worldwide and are responsible for severe damage to the child in from one to five newborns per 20,000 births. animal models of congenital cmv infection resulting in disease have been developed in mice and guinea pigs. we report here the development of ventricular dilatation and leptomeningitis in rhesus monkeys, macaca mulatta, following intrauterine infection with rhesus cytomegalovirus (rcmv). central nervous system (cns) lesions were associated with low c ... | 1986 | 3016936 |
| constitutive and retinoic acid-inducible expression of cytomegalovirus immediate-early genes in human teratocarcinoma cells. | human teratocarcinoma stem cells are nonpermissive for human cytomegalovirus (hcmv) but become permissive after being induced to differentiate by treatment with retinoic acid. we show that in uninduced teratocarcinoma stem cells, and also in transformed human 293 cells expressing adenovirus e1a gene products, the hcmv immediate-early (ie) 68,000-molecular-weight polypeptide (68k polypeptide) was not expressed, and consequently input viral genomes were not replicated. however, after differentiati ... | 1986 | 3009858 |
| induction of fc (igg) receptor(s) by simian cytomegaloviruses in human embryonic lung fibroblasts. | human embryonic lung (hel) fibroblasts infected with simian cytomegalovirus (scmv) were found to bind nonspecifically to the fc portion of human immunoglobulin (ig) g (igg). binding of igg to scmv-infected hel fibroblasts, but not to uninfected hel cells, was visualized as cytoplasmic fluorescence by the indirect immunofluorescence test, regardless of the presence of anti-cmv antibodies. the receptor(s) reacted with the igg class of different species, but not with igm and iga. the purified fc fr ... | 1988 | 2852656 |
| identification of the major capsid protein gene of human cytomegalovirus. | the coding region for the major capsid protein (mcp) of human cytomegalovirus (hcmv) was identified by comparing the protein sequence with the respective sequences of herpes simplex virus (hsv), epstein-barr virus, and varicella-zoster virus. the predicted length of the hcmv mcp was 1,370 amino acids. comparison of the mcp sequences of the different human herpesviruses showed a homology of 25% to the mcp of hsv type 1, a homology of 29% to the mcp of epstein-barr virus, and a homology of 23% to ... | 1989 | 2536837 |
| novel induction by herpes simplex virus of hybrid interferon gene transcripts driven by the strong cytomegalovirus ie94 promoter. | we have constructed stable dna-transfected ltk+ cell lines containing two different coselected hybrid interferon (ifn) genes driven by the usually strong and constitutive promoter from the immediate-early 94k protein (ie94) gene of simian cytomegalovirus. surprisingly, and unlike hybrid ie94-chloramphenicol acetyltransferase gene constructs, both of the ie94-ifn genes (one with and one without the complex spliced intron region) produced relatively low basal titers of biologically active human if ... | 1987 | 2433469 |
| identification of the lytic origin of dna replication in human cytomegalovirus by a novel approach utilizing ganciclovir-induced chain termination. | infection with human cytomegalovirus in the presence of the antiviral nucleotide analog ganciclovir results in continuing low-level viral dna synthesis and the accumulation of relatively small fragments of double-stranded progency dna. these fragments consistently proved to represent amplification of sequences from only one small section of the viral genome (ecori-v) lying near the center of the unique l segment. further mapping revealed that the viral sequences represented in these fragments oc ... | 1990 | 2173786 |
| the ie2 gene products of human cytomegalovirus specifically down-regulate expression from the major immediate-early promoter through a target sequence located near the cap site. | the 82-kda ie2 protein of human cytomegalovirus (hcmv) acts as both a powerful nonspecific trans activator of heterologous promoters and a negative autoregulator of hcmv immediate-early gene expression in transient assays. we show here that the highly specific down-regulation effect occurs in permissive diploid human fibroblast cells as well as in nonpermissive vero cells and that the target sequences are conserved within the major immediate-early promoters of both hcmv and simian cytomegaloviru ... | 1990 | 2173785 |
| the palindromic series i repeats in the simian cytomegalovirus major immediate-early promoter behave as both strong basal enhancers and cyclic amp response elements. | a 600-base-pair (bp) enhancer region upstream from the major ie94 gene of simian cytomegalovirus (scmv) produces very strong basal expression of associated gene products. this domain consists of multiple sets of interspersed repetitive elements, including 11 copies of a conserved 16-bp palindromic sequence with the consensus ccattgacgtcaatgg. these series i repeats contain an 8-bp core tgacgtca that resembles the cyclic amp (camp) response element (cre) of cellular genes. in transient chloramphe ... | 1990 | 2152815 |
| identification of a transactivating function mapping to the putative immediate-early locus of human herpesvirus 6. | sequencing studies have indicated that the unique component of the human herpesvirus 6 (hhv-6) genome and the unique long segment of the human cytomegalovirus genome are genetically colinear. of particular interest is the identification of a region of local cpg dinucleotide suppression in the genome of hhv-6, a feature conserved in the genomes of human cytomegalovirus, murine cytomegalovirus, and simian cytomegalovirus, and a characteristic of the major immediate-early loci of these viruses. adj ... | 1991 | 1654446 |
| cytomegalovirus assembly protein nested gene family: four 3'-coterminal transcripts encode four in-frame, overlapping proteins. | the genomic region encoding the assembly protein of simian cytomegalovirus (cmv) strain colburn has been cloned, sequenced, and found to be organized as a nested set of four in-frame, 3'-coterminal genes, each with its own tata promoter element and translational start codon, and all using a single 3' polyadenylation signal. the 3' end of the longest open reading frame (1.770 bp) was identical to the 930-bp sequence coding for the assembly protein precursor, as determined from a cdna clone. the a ... | 1991 | 1649317 |
| herpes simplex virus infection selectively stimulates accumulation of beta interferon reporter gene mrna by a posttranscriptional mechanism. | to study the mechanism of a novel herpes simplex virus (hsv) activity that stimulates expression of reporter genes containing beta interferon (ifn-beta)-coding sequences, we have established permanent dna-transfected cell lines that each contain two distinct hybrid genes encoding mrna species with different half-lives. these reporter genes comprised either the human ifn-beta- or bacterial chloramphenicol acetyltransferase (cat)-coding and 3' untranslated regions placed under the transcriptional ... | 1992 | 1316484 |
| antibodies in urine of chimpanzees with chronic adenoviral viruria. | many chimpanzees have naturally occurring chronic intermittent viruria with an adenovirus of a new type called pan 11. small amounts of neutralizing antibodies to pan 11 adenovirus were found in the urine of chimpanzees. urinary antibodies to adenovirus were mainly of the immunoglobulin g (igg) class with some iga antibodies also present. there was no neutralizing activity in urine against another adenovirus, pan 9, which has been isolated from lymph nodes, but not from urine, of chimpanzees; ho ... | 1978 | 211084 |
| simian cytomegalovirus-related stealth virus isolated from the cerebrospinal fluid of a patient with bipolar psychosis and acute encephalopathy. | a cytopathic 'stealth' virus was cultured from the cerebrospinal fluid of a patient with a bipolar psychotic disorder who developed a severe encephalopathy leading to a vegetative state. dna sequencing of a polymerase chain reaction-amplified product from infected cultures has identified the virus as an african green monkey simian cytomegalovirus (scmv)-related stealth virus. the virus is similar to the scmv-related stealth virus isolated from a patient with chronic fatigue syndrome. the finding ... | 1996 | 8888270 |
| acute encephalopathy induced in cats with a stealth virus isolated from a patient with chronic fatigue syndrome. | a simian cytomegalovirus-related stealth virus, isolated from a patient with the chronic fatigue syndrome, induced an acute neurological illness when inoculated into cats. histological examination of brain tissue showed foci of cells with cytoplasmic vacuolization and an absence of any inflammatory reaction. electron microscopy confirmed the presence of herpes-like viral particles and viral-like products in the brain of an inoculated animal. these findings support the role of stealth viruses in ... | 1995 | 8821627 |
| rat cytomegalovirus has a unique immediate early gene enhancer. | the immediate early (ie) enhancer/promoter regions of human cytomegalovirus (hcmv), simian cytomegalovirus (scmv), and murine cytomegalovirus (mcmv) share the following characteristics: (1) they demonstrate high-level transcriptional properties, (2) multiple repetitive elements are found throughout the enhancer region, and (3) consensus binding sites for cellular transcription factors are frequently located within the repetitive elements. here we characterize the enhancer/promoter region of the ... | 1996 | 8806515 |
| identification of a large bent dna domain and binding sites for serum response factor adjacent to the nfi repeat cluster and enhancer region in the major ie94 promoter from simian cytomegalovirus. | the major immediate-early (mie) transactivator proteins of cytomegaloviruses (cmv) play a pivotal role in the initiation of virus-host cell interactions. therefore, cis- and trans-acting factors influencing the expression of these proteins through their upstream promoter-enhancer regions are important determinants of the outcome of virus infection. s1 nuclease analysis and in vitro transcription assays with the mie (or ie94) transcription unit of simian cmv (scmv) (colburn) revealed a single pro ... | 1993 | 8380090 |
| herpesvirus proteinase: site-directed mutagenesis used to study maturational, release, and inactivation cleavage sites of precursor and to identify a possible catalytic site serine and histidine. | the cytomegalovirus maturational proteinase is synthesized as a precursor that undergoes at least three processing cleavages. two of these were predicted to be at highly conserved consensus sequences--one near the carboxyl end of the precursor, called the maturational (m) site, and the other near the middle of the precursor, called the release (r) site. a third less-well-conserved cleavage site, called the inactivation (i) site, was also identified near the middle of the human cytomegalovirus 28 ... | 1993 | 8230459 |
| screening donors for xenotransplantation. the potential for xenozoonoses. | xenotransplantation is a potential solution to the current donor shortage for solid organ transplantation. the transmission of infectious agents from donor organs or bone marrow to the recipient is a well-recognized phenomenon following allotransplantation. thus the prospect of xenotransplantation raises the issue of xenozoonoses--i.e., the transmission of animal infections to the human host. anticipating an increasing number of baboon to human transplants, 31 adult male baboons (papio cynocepha ... | 1994 | 8197609 |
| the location and sequence composition of the murine cytomegalovirus replicator (orilyt). | dna replication during human or simian cytomegalovirus (cmv) infection has been shown to be under control of a replicator region referred to as orilyt. the murine cmv orilyt has been mapped to a region of the genome located upstream of the gene encoding the herpesvirus-conserved single-stranded dna binding protein, analogous to human and simian cmv orilyts. a minimal orilyt of approximately 1.7 kbp has been identified using a transient replication system. like occurs with human and simian cmv co ... | 1997 | 9143291 |
| detection of rna sequences in cultures of a stealth virus isolated from the cerebrospinal fluid of a health care worker with chronic fatigue syndrome. case report. | a cytopathic stealth virus was cultured from the cerebrospinal fluid of a nurse with chronic fatigue syndrome. reverse transcriptase-polymerase chain reaction (rt-pcr) performed on the patient's culture yielded positive results with primer sets based on sequences of a previously isolated african green monkey simian-cytomegalovirus-derived stealth virus. the same primer sets did not yield pcr products when tested directly on dna extracted from the cultures. the findings lend support to the possib ... | 1997 | 9200191 |
| cellular sequences in stealth viruses. | cloned dna obtained from the culture of an african green monkey simian cytomegalovirus-derived stealth virus contains multiple discrete regions of significant sequence homology (p values ranging from 4 x 10(-3) to 1 x 10(-20)) to portions of known human cellular genes. the stealth virus was cultured from a patient with chronic fatigue syndrome (cfs). earlier studies had revealed considerable sequence heterogeneity within dna fragments isolated from virus-infected cells. a set of polymerase chain ... | 1998 | 9645627 |
| capsid structure of simian cytomegalovirus from cryoelectron microscopy: evidence for tegument attachment sites. | we have used cryoelectron microscopy and image reconstruction to study b-capsids recovered from both the nuclear and the cytoplasmic fractions of cells infected with simian cytomegalovirus (scmv). scmv, a representative betaherpesvirus, could thus be compared with the previously described b-capsids of the alphaherpesviruses, herpes simplex virus type 1 (hsv-1) and equine herpesvirus 1 (ehv-1), and of channel catfish virus, an evolutionarily remote herpesvirus. nuclear b-capsid architecture is ge ... | 1999 | 9971801 |
| herpes simplex virus vector-mediated expression of bcl-2 prevents 6-hydroxydopamine-induced degeneration of neurons in the substantia nigra in vivo. | 6-hydroxydopamine (6-ohda) is widely used to selectively lesion dopaminergic neurons of the substantia nigra (sn) in the creation of animal models of parkinson's disease. in vitro, the death of pc-12 cells caused by exposure to 6-ohda occurs with characteristics consistent with an apoptotic mechanism of cell death. to test the hypothesis that apoptotic pathways are involved in the death of dopaminergic neurons of the sn caused by 6-ohda, we created a replication-defective genomic herpes simplex ... | 1999 | 10097166 |
| stealth adaptation of an african green monkey simian cytomegalovirus. | dna extracted from cultures of a cytopathic virus isolated from a patient with chronic fatigue syndrome was cloned into pbluescript plasmid. the nucleotide sequences of the plasmid inserts were analyzed using the blastn and blastx programs of the national center for biotechnology information. in confirmation of earlier studies, many of the sequences show partial homology to various regions within the genome of human cytomegalovirus (hcmv). the matching regions were unevenly distributed throughou ... | 1999 | 10331958 |
| bacteria-related sequences in a simian cytomegalovirus-derived stealth virus culture. | extensive sequencing of cloned dna isolated from the culture of an african green monkey simian cytomegalovirus-derived stealth virus has identified multiple regions of highly significant homology to various bacterial genes. the apparent acquisition of bacterial sequences extends the potential role of stealth viruses as natural vectors in the transfer of genetic information. the findings highlight the dynamic interface between viral and bacterial genomes and the potential of this interaction in t ... | 1999 | 10331959 |
| melanoma growth stimulatory activity (mgsa/gro-alpha) chemokine genes incorporated into an african green monkey simian cytomegalovirus-derived stealth virus. | dna isolated from the culture of an african green monkey simian cytomegalovirus (scmv)-derived stealth virus has been cloned. a region of the virus that contains genes coding proteins homologous to the ul141, ul144, and ul145 proteins of human cytomegalovirus has recombined with cellular sequences encoding several distinct copies of the melanoma growth stimulatory activity (mgsa/gro-alpha) chemokine gene. this finding illustrates the capacity of stealth viruses to capture, amplify, and mutate ge ... | 1999 | 10331960 |
| phosphorylation of simian cytomegalovirus assembly protein precursor (papng.5) and proteinase precursor (papng1): multiple attachment sites identified, including two adjacent serines in a casein kinase ii consensus sequence. | the assembly protein precursor (pap) of cytomegalovirus (cmv), and its homologs in other herpesviruses, functions at several key steps during the process of capsid formation. this protein, and the genetically related maturational proteinase, is distinguished from the other capsid proteins by posttranslational modifications, including phosphorylation. the objective of this study was to identify sites at which pap is phosphorylated so that the functional significance of this modification and the e ... | 1999 | 10516011 |
| novel tat-encoding bicistronic human immunodeficiency virus type 1-based gene transfer vectors for high-level transgene expression. | we describe bicistronic single-exon tat (72-amino-acid tat [tat72])- and full-length tat (tat86)-encoding gene transfer vectors based on human immunodeficiency virus type 1 (hiv-1). we created versions of these vectors that were rendered rev independent by using the constitutive transport element (cte) from mason-pfizer monkey virus (mpmv). tat72-encoding vectors performed better than tat86-expressing vectors in gene transfer experiments. cte-containing vectors, produced in a rev-independent pac ... | 2000 | 10864682 |
| chemokine receptor-related genetic sequences in an african green monkey simian cytomegalovirus-derived stealth virus. | the us28 gene of human cytomegalovirus (hcmv) codes a cell surface receptor for both beta chemokine and fractalkine molecules. this receptor facilitates hcmv-induced cell fusion and virus dissemination and influences susceptibility to infection with other viruses, including the human immunodeficiency virus. five adjacent but divergent open reading frames that potentially code for molecules related to the us28 protein of hcmv are present in an african green monkey simian cytomegalovirus-derived s ... | 2000 | 10891288 |
| inhibition of choroidal neovascularization by intravenous injection of adenoviral vectors expressing secretable endostatin. | endostatin is a cleavage product of collagen xviii that inhibits tumor angiogenesis and growth. interferon alpha2a blocks tumor angiogenesis and causes regression of hemangiomas, but has no effect on choroidal neovascularization (cnv). therefore, inhibitors of tumor angiogenesis do not necessarily inhibit ocular neovascularization. in this study, we used an intravenous injection of adenoviral vectors containing a sig-mendo transgene consisting of murine immunoglobulin kappa-chain leader sequence ... | 2001 | 11438478 |
| live oral poliovirus vaccines and simian cytomegalovirus. | live oral poliovirus vaccines (opv) are often produced in primary cercopithecus monkey kidney (cmk) cells. the kidneys of these monkeys are often latently infected with simian cytomegalovirus (scmv), and cmk cultures are frequently contaminated with scmv. we tested human, monkey and rabbit tissue culture systems, and found that mrc-5 cells are most sensitive for detection of scmv. to address the question of whether opv could be contaminated with infectious scmv, we inoculated mrc-5 cells with ne ... | 2002 | 12217341 |
| simian cytomegalovirus and contamination of oral poliovirus vaccines. | in the 1950s the use of primary rhesus macaque kidney cultures to propagate poliovirus for vaccine production led to the contamination of vaccines with simian virus 40 (sv40). african green monkey kidney (agmk) cultures free of sv40 were used as an alternative cell substrate for vaccine manufacture. in this study we evaluate oral poliovirus seeds, vaccine bulks and vaccines themselves for the presence of a common contaminant of agmk cultures, simian cytomegalovirus (scmv). using sensitive polyme ... | 2003 | 12623061 |
| two novel spliced genes in human cytomegalovirus. | two novel spliced genes (ul131a and ul128) flanking ul130 were predicted from sequence comparisons between human cytomegalovirus (hcmv) and its closest known relative, chimpanzee cytomegalovirus (ccmv), and the splicing patterns were confirmed by mrna mapping experiments. both genes were transcribed with late kinetics and shared a polyadenylation site. comparisons with wild-type hcmv in infected human tissues showed that three of five isolates passaged in cell culture contained disruptions of ul ... | 2003 | 12692276 |
| simian cytomegalovirus encodes five rapidly evolving chemokine receptor homologues. | many herpesviruses, poxviruses and retroviruses encode proteins related to chemokines and chemokine receptors. the first one discovered, us28 of human cytomegalovirus (hcmv), is a 7-transmembrane domain g protein-coupled chemokine receptor able to activate diverse cellular responses, including cell migration and gene expression. a related orf named us27 is adjacent to us28, but no functions have been defined yet. recently orfs 3-7, a cluster of five concatenated orfs with highest homology to us2 ... | 2004 | 14739653 |
| cell lineage tracing during xenopus tail regeneration. | the tail of the xenopus tadpole will regenerate following amputation, and all three of the main axial structures - the spinal cord, the notochord and the segmented myotomes - are found in the regenerated tail. we have investigated the cellular origin of each of these three tissue types during regeneration. we produced xenopus laevis embryos transgenic for the cmv (simian cytomegalovirus) promoter driving gfp (green fluorescent protein) ubiquitously throughout the embryo. single tissues were then ... | 2004 | 15148301 |
| properties of virion transactivator proteins encoded by primate cytomegaloviruses. | human cytomegalovirus (hcmv) is a betaherpesvirus that causes severe disease in situations where the immune system is immature or compromised. hcmv immediate early (ie) gene expression is stimulated by the virion phosphoprotein pp71, encoded by open reading frame (orf) ul82, and this transactivation activity is important for the efficient initiation of viral replication. it is currently recognized that pp71 acts to overcome cellular intrinsic defences that otherwise block viral ie gene expressio ... | 2009 | 19473490 |
| establishment of specific pathogen-free macaque colonies in tsukuba primate research center of japan for aids research. | cynomolgus monkeys have been maintained in indoor facilities as closed colony monkeys in tsukuba primate research center in japan since 1978. several microorganisms, including bacteria, parasites and viruses, were eliminated from the cynomolgus monkeys in this colony of tprc. various kinds of viruses (b virus, measles virus, simian varicella virus, simian immunodeficiency virus, simian t cell leukemia virus, simian d type retrovirus, simian cytomegalovirus, simian epstein-barr virus, and simian ... | 2010 | 20510748 |
| the scmv ie enhancer/promoter system for high-level expression and efficient functional studies of target genes in mammalian cells and zebrafish. | human and simian cytomegalovirus immediate-early (ie) enhancer/promoter (hcmvp and scmvp) are the most widely used system for high-level gene expression; however, studies on detailed comparative analyses of the promoters are scarce. using gfp reporter gene and immunoblotting assays, we have shown that the transcriptional activity of scmvp was two to four fold higher than those of hcmvp in human-, monkey-, mouse-originated cell lines, and zebrafish as a vertebrate animal model. notably, htra1 dri ... | 2011 | 21424167 |
| assembly protein precursor (pul80.5 homolog) of simian cytomegalovirus is phosphorylated at a glycogen synthase kinase 3 site and its downstream "priming" site: phosphorylation affects interactions of protein with itself and with major capsid protein. | capsid assembly among the herpes-group viruses is coordinated by two related scaffolding proteins. in cytomegalovirus (cmv), the main scaffolding constituent is called the assembly protein precursor (pap). like its homologs in other herpesviruses, pap is modified by proteolytic cleavage and phosphorylation. cleavage is essential for capsid maturation and production of infectious virus, but the role of phosphorylation is undetermined. as a first step in evaluating the significance of this modific ... | 2004 | 15564461 |
| african green monkey origin of the atypical cytopathic 'stealth virus' isolated from a patient with chronic fatigue syndrome. | a cytomegalovirus-like 'stealth virus' had previously been isolated from a patient with the chronic fatigue syndrome (cfs). | 1995 | 15566831 |