| comparative study of aujeszky's disease virus reproduction in a suspension of poultry embryonic cells and tissues (author's transl). | reproduction of aueski disease virus in suspension cultures of trysinized cells and mechanically minced tissue of chick, duck and quail embryos was compared. the optimal conditions for cultivation of vaccine and virulent strains of virus in these systems were determined. the advantages and prospects of using suspension cultures of minced avian embryo tissue for preparation of virus materials with high biological activity and in large volumes in comparison with trypsinized cell suspensions were d ... | 1975 | 1907 |
| infection of hela cells with a virulent and an attenuated strain of pseudorabies virus studied by electron microscopy. | hollow-core particles, forming crystals in nuclei, prevailed in hela cells infected with an attenuated strain of pseudorabies virus (prv). after infection with a virulent prv strain, the cells contained mainly fully infectious dense-core particles. these findings might explain the lower susceptibility of hela and some other human cells to infection with attenuated strains of prv as compared to virulent strains. | 1976 | 9806 |
| stability in drinking and surface water of nine virus species from different genera (author's transl). | the stability of nine viruses, aujeszky, sindbis, vesicular stomatitis, newcastle disease, vaccinia, fmd, hcc, reo and teschen virus in drinking and surface water was investigated comparatively at temperatures of 9 and 15 degrees c as well as the influence of water factors like seasonal difference in temperature, ph value, hardness and sort of water. the results can be summarized as follows: 1. at temperatures of 9 to 15 degrees c the majority of the viruses remained stabil in natural water for ... | 1977 | 14460 |
| heterogeneity of pseudorabies virus studied by isoelectric separation. | heterogeneity of four lines derived from a single strain of pseudorabies virus (prv) was studied by isoelectric separation. the individual lines differed in the distribution pattern of infectious fractions separated in the ph range from 3.5 to 10.0. all virus fractions of the virulent and attenuated lines were infectious, but some fractions of the highly attenuated avirulent line were devoid of infectivity. infectivity of the fractions isolated from the individual pvr lines depended on the degre ... | 1977 | 18917 |
| antiviral activity of dipyridamole derivatives. | among 46 novel pyrimido 5.4-d pyrimidine derivatives, 26 compounds were found to exhibit antiviral activity as revealed in a test programme against mengo, coxsackie b1, fowl plague, vaccinia and pseudorabies viruses, as concerns inhibition of plaque formation and of infectious virus yield. attempts to disclose structure-activity relationships by discriminant analysis pointed to a possible importance of hydrophobic substitution for the antiviral effectiveness against mengo virus of the derivative ... | 1978 | 29467 |
| [effect of passive immunity on subsequent vaccination against swine fever]. | studied was the effect of a serum paratyph and aujeszky gammaglobulin injected 10, 15, and 20 days prior to the crystal-violet and the lapinized vaccine. if the animals were infected with a pathogenic swine fever virus 10 to 15 days after their treatment with serum at the rate of 0.5 cu. cm per kg, part of them died, and the remaining contracted the disease showing a protracted course. the control vaccination on the 20th day after the animals were injected with serum led to the death of all of t ... | 1975 | 53938 |
| isolation of a nucleocapsid polypeptide of herpes simplex virus types 1 and 2 possessing immunologically type-specific and cross-reactive determinants. | a polypeptide (p40) of approximately 40,000 molecular weight was isolated from herpes simplex virus type 1 and 2 nucleocapsids by gel filtration and ion exchange chromatography. this protein appears to be the same as protein 22a described previously (gibson and roizman, j. virol. 10:1044--1052, 1972). competition immunoassays were developed by using purified p40 and antisera prepared in guinea pigs. the assays indicated that the p40's from herpes simplex virus types 1 and 2 possess both type-spe ... | 1979 | 85720 |
| [colostral immunity and immunoprophylaxis in aujeszky's disease]. | | 1978 | 88789 |
| virus development in enucleate cells: echovirus, poliovirus, pseudorabies virus, reovirus, respiratory syncytial virus and semliki forest virus. | a group of rna viruses, echovirus, poliovirus, reovirus, respiratory syncytial virus and semliki forest virus have been examined for ability to grow in enucleate african green monkey kidney (bsci) cells. semliki forest virus produced an almost normal yield of virus but poliovirus, echovirus, reovirus and respiratory syncytial virus, although showing clear evidence of virus replication when compared with a nuclear dna virus (pseudorabies virus) gave much lower yields than those from nucleate cell ... | 1975 | 163289 |
| the densities of herpesviral dnas. | | 1975 | 164053 |
| viral infection and interferon in cell cultures aged in vitro. | cells from leucosis-free chick embryos (skin and muscles), grown in vitro 1 and 7 days with no medium change, are used as a model for young dividing and stationary in vitro aged cells, respectively. aged cultures are advantageous for if induction by cytopathic and noncytopathic viruses. decreased virus production in them is ascribed to a back effect of the early formed if onto the inducing virus. a factor which stimulates viral if interferon induction also in young cultures, is released from age ... | 1975 | 164104 |
| studies on immunity to aujeszky's disease (pseudorabies) virus infection in pigs. | aujeszky's disease is primarily a disease of pigs caused by a herpesvirus. in pigs, the primary site of virus replication is the nasopharynx. some strains cause lesions only in the central nervous system, others cause rhinitis, pneumonia and encephalitis. colostrum from immune sows protected 3 day old piglets from experimental infection, but did not prevent the piglets excreting virus. colostral antibodies were 11-16-fold greater than the serum titres in the sows and the half life of passiveanti ... | 1975 | 165131 |
| early functions of the genome of herpesvirus. v. serological analysis of "immediate-early" proteins. | | 1975 | 165618 |
| pseudorabies virus - induced neural hyperreactivity following occular and skin infections in the rat. | the types of functional disturbances caused by a neurotropic virus was investigated by means of neurophysiological techniques. two types of neurophysiologic lesions were produced in the superior cervical sympathetic ganglia and sural nerve in rats infected with two distinct strains of pseudorabies virus. the pruritus-producing strain following intraocular inoculation displayed spontaneous activity in the ganglia both in vivo and in vitro. this activity was susceptible to the actions of atp, amp, ... | 1975 | 166421 |
| [comparison of the sensitivity of laboratory animals and tissue cultures to infection with aujeszky's disease virus]. | in mice and guinea-pigs high susceptibility was demonstrated following i. c. administration of the virus, approximately equalling to that of rabbits and tissue cultures. also a relatively high susceptibility of guinea-pigs was demonstrated, with very distinct clinical symptoms of the disease, as compared with mice and rats after the other manners of infection. on the basis of the results obtained white mice were utilized for routine diagnostic of aujeszky disease. after i. c. administration of p ... | 1975 | 167500 |
| further characterization of a population of defective, interfering pseudorabies virions. | | 1975 | 167515 |
| the effects of protein deprivation on cell-mediated immunity. | the effect of protein insufficiency on immune responsiveness was investigated in inbred mice fed isocaloric diets low and normal in protein. mice were tested for aberrations of cell-mediated and humoral immunities. protein insufficiency enhances cell-mediated immunity while depressing or not affecting humoral immunity. cell-mediated immunity was enhanced in testing: allogeneic skin graft rejection, spleen cell responsiveness to phytohemagglutinin, the graft-vs-host reaction, macrophage function, ... | 1975 | 167880 |
| prevalence of pseudorabies in sows in central illinois. | of 1,224 serums from slaughtered sows from central illinois, 52 (4.25%) were found to be positive for pseudorabies neutralizing antibody. | 1975 | 168171 |
| streptovirudins, new antibiotics with antibacterial and antiviral activity. i. culture taxonomy, fermentation and production of streptovirudin complex. | a new antibiotic complex has been isolated from cultures of streptomyces strain no. ja 10124. on the basis of taxonomic studies, the producing microorganism is described as streptomyces griseoflavus (krainsky, 1914) waksman et henrici, 1948, subsp. thuringiensis subsp. nov., type strain ja 10124. the antibiotic complex, designated as streptovirudin, was isolated from extracts of both mycelium and culture filtrate. it is a white amorphous material which consists of ten closely related components ... | 1975 | 168173 |
| effect of 2-deoxy-d-glucose on herpesvirus-induced inhibition of cellular dna synthesis. | in pseudorabies virus-infected cells host dna synthesis is turned off 4 to 5 h postinfection. in the presence of 0.5 mm 2-deoxy-d-glucose, however, synthesis of both cellular and viral dna proceeds unimpaired throughout the virus replication cycle. the uptake of radioactive thymidine into mock-infected cells is not altered in the presence of 2-deoxy-d-glucose. virus-specific protein synthesis and particle formation also proceed in medium containing the deoxy sugar, but the virus particles produc ... | 1975 | 168399 |
| [an atypical case of aujeszky's disease in a dog (author's transl)]. | two cases of aujeszky's disease in a cat and a dog belonging to the same owner are reported. the two animals each were five months of age. the symptoms shown by the cat were typical of aujeszky's disease: intense itching, salivation and the head bent to one side. the main symptoms shown by the dog consisted in salivation, ptosis of one eye, a drooping ear, the head bent to one side and ataxia. as itching was not observed in the dog and the animal had spent the first months of its life in wooded ... | 1975 | 168663 |
| mitochondrial and herpesvirus-specific deoxypyrimidine kinases. | to characterize and compare the thymidine (tdr) and deoxycytidine (cdr) kinase isozymes of uninfected and herpesvirus-infected cells: (i) the subcellular distribution of the isozymes has been studied; (ii) a specific assay for cdr kinase has been devised; (iii) the tdr kinase isozymes have been partially purified; and (iv) the purified enzymes have been analyzed by disc polyacrylamide gel electrophoresis, isoelectric focusing, and glycerol gradient centrifugation and by substrate competition and ... | 1975 | 169387 |
| [bovine viruses and methods for their detection]. | | 1975 | 169627 |
| virus-induced proteins in pseudorabies-infected cells. ii. proteins of the virion and nucleocapsid. | analysis of purified naked and enveloped nucleocapsids of pseudorabies virus with high-resolution techniques has allowed a reassessment of their protein composition. enveloped particles are shown to contain at least 20 proteins whose molecular weights are in the range 20,000 to 230,000. naked nucleocapsids contain one major and seven minor proteins in the molecular weight range 20,000 to 155,000. phosphorylation of at least one virion protein is shown to take place in vivo. these results demonst ... | 1975 | 170424 |
| [in vitro evaluation of antiviral inhibitors by means of a turbidity method (author's transl)]. | the paper presents a photometric method for the determination of virus-induced alterations (cytopathogenic effects) of cells based on nephelometric analysis. these were taken at varying time intervals after infection and with different multiplicity of infection. it was demonstrated that suppression of virus-induced cpe by the known inhibitors could also be followed by turbidity measurements. the in vitro experiments were carried out in the 2 following virus-cell-systems: pseudorabies virus in ch ... | 1975 | 171906 |
| [suitability of immune serums from different animal species for the preparation of conjugates against aujeszky's disease virus]. | a comparison was made of immune serum from rabbits, sheeps, pigs and cattle. pure igg was prepared by the rivanol sulphate method and immune electrophoresis. optimum protein quality, conjugation time under various conditions and using two preparations of fluorescein isothiocyanate were studied. conjugate purified by deae-sephadex a-50 and concentrated to 1% protein by ultrafiltration was freeze-dried and then tested for activity and the degree of non-specific fluorescence. evaluation by yield of ... | 1975 | 172039 |
| effect of tetraethyl thiuram disulfide (disulfiram) on the multiplication of enveloped viruses. | disulfiram at concentrations between 0.1 and 0.3 mm inhibits the multiplication of semliki forest virus (sfv), fowl plague virus (fpv), newcastle disease virus (ndv), vesicular stomatitis virus (vsv), and pseudorabies virus (prv), when administered 1 hour before and during adsorption. there is, however, no inhibition of virus multiplication, when the drug is added after adsorption onto chick embryo cells. disulfiram interferes neither with the receptors of the virus nor of erythrocytes, and it d ... | 1975 | 173261 |
| dna polymerase in pseudorabies virus infected cells. | the dna polymerase activity in bhk 21/c13 cells infected with pseudorabies virus is inhibited by incubation with antiserum to pseudorabies but not by incubation with pre-immune serum or by antiserum to herpes simplex virus type 1 (hsv-1) or herpes simplex virus type 2 (hsv-2). it also differs from the cell enzyme and the enzymes in hsv-1 or hsv-2 infected cells in its requirement for kcl in the in vitro assay. it seems likely, therefore, that pseudorabies virus specifies its own dna polymerase. | 1976 | 173798 |
| [development of a production method of reproducing the aujeszky's disease virus]. | | 1975 | 174270 |
| [study of the symplast-forming activity of terpene and standard strains of aujeszky's disease virus]. | studied were the cellular changes and, more specifically, the production of symplasts as a particular form of manifesting the cytopathic effect of some strains of the aujezsky's virus. experiments with eight strains of the virus in tissue cultures confirmed the concept of a number of authors that in given tissue cultures virulent strains show a well pronounced trent toward the formation of symplasts containing numerous nuclei, while the latent strains stimulate the production of sporadic, rounde ... | 1975 | 174277 |
| [porperties of a strain of herpesvirus suis isolated in cases with various epizootologic and clinical courses of the disease]. | a varying epizootologic, clinical and morphologic picture was established in studying the course of the disease on six pig-breeding farms. the isolated strains showed differences in their virulence, cultural properties and physicochemical behaviour. two of them had lowered virulence, and one showed affinity to the respiratory organs which corresponded to the peculiarities and course of the disease. it is concluded that the interaction between the virulence of the strains and the herd immunity re ... | 1975 | 174278 |
| [immune ascitic fluid for aujeszky's disease virus]. | | 1975 | 175543 |
| [aujeszky's disease virus accumulation in a culture of isolated porcine kidneys]. | | 1975 | 175546 |
| [obtaining the beta-globulin fractions from swine and cattle sera and a study of their immunological activity]. | the rivanol precipitation was used to obtain beta-globulin fractions from specific swine sera against edema disease, paratyph and aujeszky's disease as well as from normal ovine and swine sera. agar electrophoresis revealed that the preparations produced contained beta-globulin (86 per cent), gamma-globulin (5 per cent), and alpha2-globulin (9 per cent). the beta-globulin preparations were studied for the presence of antibodies against e. coli, salmonellae, staphylococci, myxovirus parainfluenza ... | 1976 | 176771 |
| concatemeric forms of intracellular herpesvirus dna. | | 1976 | 176785 |
| effect of infection with enveloped viruses on nucleotide metabolism. | | 1976 | 176807 |
| the absence of one class of virus-induced protein in arginine-deprived cells infected with pig herpesvirus. | | 1976 | 176814 |
| cell-mediated immunity in aujeszky disease virus infected pigs. i. lymphocyte stimulation. | the appearance of cell-mediated immunity was studied in aujeszky diseased pigs with the aid of the in vitro stimulation of sensitized lymphocytes. the first cell-mediated immunity reaction of lymphocytes occurred 4 days after infection. from day 7 to 35, the latest day tested, the reactions were most marked with lymphocytes from lymph nodes and spleen, whereas blood and thymus lymphocytes reacted less frequently; bone marrow lymphocytes showed no response. reinfection did not considerably enhanc ... | 1976 | 176973 |
| synthesis of proteins in cells infected with herpesvirus. xi. sulfated, structural glycoproteins. | | 1976 | 178100 |
| [use of temperature, trypsin and biological tests for the intraspecies differentiation of local strains of aujeszky's disease virus]. | studies were carried out with 8 strains of aujeszky's disease virus using a temperature test (50 degrees c for 1 hour), a trypsin test (0.5 mg per cu. cm at 37 degrees c 1 hour), and a biologic test. the strains were isolated from the brain of dead pigs that have presumably been affected with encephalitis. they proved sensitive when studied with the temperature and the trypsin tests sharply lowering their titer. the viruses isolated from pneumonic foci in the lungs of pigs in which the disease h ... | 1975 | 179192 |
| fate of parental herpesvirus dna. | | 1976 | 180660 |
| a pseudorabies virus-specific sulfated moiety. | | 1976 | 180663 |
| [aujezky's disease in the field and vaccination against the disease (author's transl)]. | a rather extensive outbreak of aujeszky's disease in the area served by the above veterinarians is reported. of 252 pig-breeding farms, eighty-two were contaminated. the other herds (170) were partly (81) vaccinated against the disease with the bartha strain. unlike de non-vaccinated group, the immunized herds showed few of any losses. in view of this fact it is concluded that aujeszky's disease vaccination is a useful procedure (even in a contaminated environment). | 1976 | 181864 |
| [agar gel diffusion precipitation and immunoelectrophoresis of the aujeszky virus]. | two virulent and two vaccinal virus strains are dealt with. the study has been carried out by means of the diffusion precipitation reaction in agar gel and the immunoelectrophoresis technique using hyperimmune sera obtained from cocks as well as concentrated and purified antigens. it has been demonstrated that the virulent and the vaccinal (latent) strains of aujeszky's disease virus cannot be differentiated serologically. the complex of antibodies induced by the virulent and the latent strains ... | 1976 | 181894 |
| [action of rifampicin on the reproduction of aujeszky's disease virus]. | the study on the effect of rifampicin on the reproduction of aujeszky's disease virus (strains 2, 5, 7, 10, 12, and 17, which are virulent, and strain mk, which is an avirulent virus mutant) in cell cultures revealed that the formation of plaques is inhibited, though to a slighter extent, at a concentration of the inhibiting agent of 100 mug/ml, the value of their titer lowering by one logarithm. all virus strains have proved equally susceptible to the effect of rifampicin. during the virus mult ... | 1976 | 181898 |
| a comparison of two populations of defective, interfering pseudorabies virus particles. | | 1976 | 181909 |
| a comparative examination of swine sera for antibody to aujeszky virus with the conventional and a modified virus-serum neutralization test and a modified direct complement fixation test. | | 1976 | 181975 |
| [serological studies on the distribution of aujeszky virus in pig-breeding farms in the weser-ems area]. | | 1976 | 182448 |
| fluorosugars inhibit biological properties of different enveloped viruses. | both 2-deoxy-2-fluoro-d-glucose and 2-deoxy-2-fluoro-d-mannose were found to be potent inhibitors of the synthesis of infectious semliki forest and fowl plague virus in chicken embryo cells and also of pseudorabies virus grown in rabbit kidney cells. it was found that the pseudorabies virus-mediated cell fusion and the synthesis of functional hemagglutinin of fowl plague virus were blocked. in all cases the 2-deoxy-2-fluoro-d-mannose-caused inhibition was stronger than the 2-deoxy-2-fluoro-d-glu ... | 1976 | 178923 |
| [study of aujeszky's disease virus replication by using an immunofluorescent method]. | studied was the interrelationship between the aujeszky's disease virus (the avirulent mutant strain mk and the virulent strain 2) and chick embryo cells during cultivation with and without 5-iod-2-desoxiuridine. in experiments with the virulent strain the presence of a granular antigen could be observed at the 4th hour following the infection of the nucleus. after the 10th hour the antigen showed migration from the nucleus toward the cytoplasm. after the 24th hour a complete destruction of the c ... | 1976 | 183344 |
| inhibition of pseudorabies virus replication by vesicular stomatitis virus. ii activity of defective interfering particles. | purified defective interfering (di) particles of vesicular stomatitis virus (vsv) inhibit the replication of a heterologous virus, pseudorabies virus (psr), in hamster (bhk-21) and rabbit (rc-60) cell lines. in contrast to infectious b particles of vsv, uv irradiation of di particles does not reduce their ability to inhibit psr replication. however, uv irradiation progressively reduces the ability of di particles to cause homologous interference with b particle replication. pretreatment with int ... | 1976 | 178895 |
| inhibition of pseudorabies virus replication by vesicular stomicles virus i. activity of infectious and inactivated b particles. | infectious b particles of vesicular stomatitis virus (vsv) are capable of inhibiting the replication of pseudorabies virus (psr) in a variety of cell lines. even under conditions of an abortive infection in a continuous line of rabbit cornea cells (rc-6o), b particles interfere with the replication of psr with high efficiency. particle per cell dose-response analysis of b particle populations revealed that the number of vsv particles capable of inhibiting psr replication exceeds the number of pf ... | 1976 | 178894 |
| [stick-plaque test--an economic method of quantitative determination of viruses]. | an economic method for quantitative assay of viruses is presented. in this "canule stick-plaque test" (german abbreviation spt) samples of viruses, geometrically diluted and taken up by a canule, are inoculated by sticking into monolayer cell cultures overlayed with agar medium. a plaquelike cpe detectable by neutral red staining develops in the area of the inoculation. the frequency of this cpe formation depends on the concentration of viruses in the inoculated dilution. this dose-response allo ... | 1976 | 186533 |
| changes in the 'melting' temperature of chromatin in cells infected with pig herpes virus 1. | | 1976 | 187500 |
| cell surface antigens induced by herpes simplex virus (hsv). | among the antigens induced by hsv on the surface of infected cells there are antigens binding anti-hsv antibodies by their combining (fab) site (hsv-ag), and others binding antibodies of various specificities, probably through their fc fragment (igr). hsv-ag were demonstrated by cytotoxicity and mixed agglutination tests; igr were made evident by passive hemadsorption. the ability to induce igr is especially characteristic of hsv-1 strains, being absent or limited with hsv-2 strains. igr were no ... | 1976 | 188230 |
| [stich-plaque test--an economic method for quantitative determination of viruses]. | an economic method for quantitative assay of viruses is presented. in this "canule stick-plaque test" (german abbreviation spt) samples of viruses, geometrically diluted and taken up by a canule, are inoculated by sticking into monolayer cell cultures overlayed with agar medium. a plaquelike cpe detectable by neutral red staining develops in the area of the inoculation. the frequency of this cpe formation depends on the concentration of viruses in the inoculated dilution. this dose-response allo ... | 1976 | 188946 |
| [effect of distamycin a on the reproduction of aujeszky's virus]. | | 1975 | 175644 |
| influence of double infections on the induction of thymidine kinase by uv-irradiated herpes simplex virus types 1 and 2 and pseudorabies virus. | simultaneous infection of primary rabbit kidney cells with hsv type 1 tk+ and a tk- strain results in a mutual influence of both viruses on the induction of thymidine kinase (tk). tk+ virus has an enhancing and tk- virus a depressing effect on tk induction by a superinfecting tk+ virus. the enzyme induction depends on the ratio of multiplicities of both viruses. the mutual influence on tk induction depends further on the time of addition of the superinfecting virus: the effect of the second viru ... | 1975 | 174520 |
| a comparative study of the neutralisation test and the indirect fluorescent antibody technique for the detection of antibodies to the virus of aujeszky in pig sera. | a microneutralisation test and an indirect fluorescent antibody technique for the detection of antibodies to aujeszky-virus in pig sera are described. all the sera were tested with both techniques and the results were compared. serum dilutions of less than 1:32 which produced a positive reaction in the indirect fat were considered nonspecific. bij contrast, all neutralising antibody titers of less than 1:8 gave nonspecific reactions in the indirect fat. | 1976 | 189453 |
| increased susceptibility to bacterial infection as a sequela of exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin. | the effects of subclinical levels of 2,3,7,8-tetrachloridibenzo-p-dioxin (tcdd) on the response of mice to infection with either salmonella bern or herpesvirus suis, also known as pseudorabies virus, are reported. tcdd is a contaminant of certain commercially useful chemicals, such as chlorinated phenols or herbicides. it has been shown to cause thymic atrophy and to suppress cell-mediated immunity in laboratory animals. sublethal levels of tcdd were used: 0.5, 1,5, 10, or 20 mug/kg, given throu ... | 1975 | 173655 |
| [possibilities of vaccination with an inactivated virus via the respiratory tract as studied in the aujesky virus--white mouse system]. | | 1977 | 189533 |
| [enzyme activity in cell cultures infected with herpesvirus suis]. | the activity of succinate dehydrogenase (sdh) and lactage dehydrogenase (ldh) was studied in chick-embryo fibroblast cultures after inoculation of the virulent strain "a2" and the avirulent strain "mk" of herpesvirus suum. strain "a2" reduced sdh activity, and so did strain mk, but here the decrease of enzyme activity was slower, and it did not become evident until the 24th hour. ldh activity fluctuated after "a2" infection but was generally increased, while there was no change in ldh activity, ... | 1975 | 172044 |
| [role of the virus of aujeszky's disease in respiratory diseases of swine]. | studies carried out on pig-breeding farms have established the presence of aujeszky's disease virus in cases of bronchopneumonia in pigs. on seven out of eight farms with bronchopneumonia the virus wad demonstrated both virologically and serologically, and nearly 50 per cent of the examined diseased pigs on these and other farms had antibodies. the individual serologic studies of pigs having antibodies revealed that the antibodies appeared in the second month of the animals' fattening. this corr ... | 1975 | 171824 |
| abnormal replication of the dna of defective pseudorabies virions. | | 1975 | 190162 |
| experimental infection of monkeys with herpesvirus suis (aujeszky's-disease virus). | monkeys were infected intranasally with herpesvirus suis. after an incubation period of 7 to 13 days the animals became acutely ill and rapidly died. clinical signs included salivation, incoordination, ataxia and epileptiform convulsions, but not pruritus. histopathological changes were confined to the central nervous system, and consisted of destruction of neurones with the formation of intranuclear inclusion bodies, gliosis and perivascular cuffing. virus was isolated from the brain and spinal ... | 1977 | 190402 |
| [an atypical form of aujezky's disease after vaccination (author's transl]. | four atypical case of aujezky's disease in dogs are described. two weeks before the outbreak of the disease, the dogs had been vaccinated with a live tissue culture vaccin, based on the bartha strain. by culturevirus (cytopathogenic effect) the bartha vaccin was identified and a vaccination reaction was proved. vaccination with this must be discouraged. | 1977 | 191953 |
| [aujeszky's disease in goats (authors transl)]. | the small number of cases of aujeszky's disease in goats referred to in the literature is stressed, and an outbreak in goats causing many deaths is reported. the flock of goats concerned was occasionally housed in a barn during the night in which there also were fattening pigs. the first deaths occurred without previous symptoms, the animals being found dead in the morning. in subsequent cases, symptoms were observed which continued for a few or several hours and consisted in agitation, lying do ... | 1977 | 191954 |
| inverted repetition in the chromosome of pseudorabies virus. | an electron microscope examination of pseudorabies virus dna single strands after self-annealing shows a loop of single-stranded dna at one end of the molecule contiguous to a double-strand region. the molecule then terminates in a further single-stranded region that does not form a loop. it is suggested that the dna contains a sequence of 13.3 x 106 daltons at one end, which is repeated internally with opposite polarity. the segment of the genome separating the repeats has a double-strand molec ... | 1977 | 192914 |
| [action of actidione on the multiplication of aujeszky's disease virus]. | studied was the effect of actidione on the propagation of the aujeszky's disease virus (strains 2,7,10--virulent,, and strain mk--avirulent virus mutant). it was found that at a concentration of 2 microng/cm3 actidione discontinues the reproduction of the most virulent strain 2 when the antibiotic was added immediately after the adsorption of the virus on the cells. the decrease in the virulence of the virus strain was associated with the decrease in the concentration of the antibiotic needed fo ... | 1976 | 193240 |
| studies on immunisation of pigs with the bartha strain of aujeszky's disease virus. | the k strain of aujeszky's disease virus (adv) grown in vero cells was used to vaccinate pigs. following intramuscular inoculation, the pigs remained healthy, no vaccine virus was excreted and virus could be detected only at the inoculation site. one inoculation gave good protection against challenge with a virulent strain of adv, and the amount of virulent adv excreted was geatly curtailed. following vaccination only low leads of serum neutralizing antibody were detected (geometric mean titre 1 ... | 1975 | 168626 |
| identification of strains of herpes simplex virus by comparison of the density of their dna using the preparative ultracentrifuge. | the buoyant densities of the dna of herpes simplex virus type 1, type 2 and pseudorabies virus, as determined in the analytical ultracentrifuge, are 1.725, 1.727 and 1.731 correlating with g+c contents of 67, 69 and 73 per cent respectively. the density differences for the dna's of type 1 and type 2 herpes simplex viruses have been confirmed in experiments with isotopically labelled dna from four type 1 and six type 2 strains by preparative cscl gradient ultracentrifugation. the dna of all the t ... | 1975 | 167692 |
| the pathogenesis of pseudorabies in mice: virus replication at the inoculation site and axonal uptake. | three-week-old mice were inoculated in the right ear pinna with pseuforabies virus. ears were surgically removed at various times after inoculation and changes from the normal pathogenesis were observed. virus replication in the ear tissue and cervical dorsal root ganglia was also monitored. followed inoculation with a small dose of virus, local multiplication of the virus was necessary before the infection spread to the nerves. with larger infecting doses there was probably direct uptake of vir ... | 1975 | 164517 |
| porcine parvovirus: frequency of naturally occurring transplacental infection and viral contamination of fetal porcine kidney cell cultures. | the frequency of naturally occurring transplacental infection of swine with porcine parvovirus (ppv) and one of the possible consequences of such infection--the presence of ppv in cell cultures prepared from fetal tissues--were investigated. transplacental infection was indicated by the presence of high titers of hemagglutination inhibiting (hi) antibody for ppv in serums of 0-day-old, hysterectomy-derived, colostrum-deprived pigs of 3 of 82 litters. all letters were farm-raised dams. moreover, ... | 1975 | 163603 |
| evaluation of pseudorabies viral antigens in the agar gel immunodiffusion test. | procedures designed to extract pseudorabies viral (prv) antigens from prv-infected tissue cultures were investigated to determine whether differences in extraction method had an effect upon the final concentrated antigenic product. all four of the preparations made from prv-infected tissue culture cells (trypsin extract and disrupted cells) or entire prv-infected cultures (polysorbate 80 extract and (nh4)2so4 precipitate) contained relatively large amounts of the same antigen, whereas cell-free ... | 1979 | 92905 |
| microbial-like antigens in human leukemia. | antigens present in leukemic sera and organ extracts, but absent from non-leukemic sera and tissues, were detected by examining their reactions with anti-microbial antisera by means of counter-immunoelectrophoresis. out of 110 different antisera used, 12 antisera, those produced against micropolyspora, coccidioides, salmonella, mycoplasma, mycobacterium simiae, absidia, pseudorabies virus and adenovirus, proved to react regularly with the leukemic sera. absorption studies point to an immunologic ... | 1978 | 85287 |
| physiological state of human embryonic lung cells affects their response to human cytomegalovirus. | cultures of human embryonic lung (hel) cells in different physiological states were studied for their susceptibility to infection with human cytomegalovirus (cmv) with respect to production of infectious virus, synthesis of viral antigens, and virus-induced stimulation of cellular dna synthesis. in general, subconfluent, actively growing cells yielded higher amounts of infectious virus than did confluent contact-inhibited cells. the higher yield of infectious virus was correlated with a greater ... | 1977 | 196097 |
| ultrastructure and function in sympathetic ganglia isolated from rats infected with pseudorabies virus. | (1) after inoculation of the pseudorabies virus in the anterior chamber of the eye of the rat, virions can be found only in the neurons of the superior cervical sympathetic ganglion and in the sensory ganglion of the fifth nerve on the inoculated side. other nervous structures--central or peripheral--are not infected. (2) it is shown that the retrograde axonal flow carries the virus from the eye to the sympathetic neurons. (3) the ultrastructure of the infected neuron has been studied at various ... | 1978 | 75040 |
| isolation of marek's disease virus dna from infected cells by electrophoresis on polyacrylamide gels. | marek's disease virus dna isolated from the nuclear fraction of infected chicken embryo fibroblasts and sucrose-purified particles was electrophoresed on 3 per cent polyacrylamide gels and was compared in its electrophoretical behaviour with isolated pseudorabies and herpes simplex dna, strain hf. the dna molecules eluted from the gel were identified by their sedimentation coefficient (53--55s) and buoyant density (1.707 g/ml) to be of viral origin. mdv dna molecules were electrophoretically als ... | 1977 | 196577 |
| immunoglobulin classes of antibodies in milk of swine after intranasal exposure to pseudorabies virus or transmissible gastroenteritis virus. | experiments were conducted to evaluate whether infection of the respiratory tract of pregnant swine with pseudorabies (pr) virus would induce the secretion of immunoglobulin a (iga) antibodies in their milk as was observed after enteric infection with transmissible gastroenteritis (tge) virus. the immune response of sows to pr virus inoculated intranasally and to tge virus inoculated orally/intranasally or via a natural infection was studied. emphasis was placed upon titers and ig classes of pr ... | 1977 | 197014 |
| [duration of survival of the vaccination strain of aujeszky's disease virus in the body of piglets]. | | 1977 | 198944 |
| [early stages of immunity following vaccination of piglets with an inactivated aujezsky's virus vaccine]. | | 1977 | 199003 |
| [studies of the cft in the diagnosis of aujeszky's disease virus in swine]. | it was found that the complement-fixation test is a prompt and suitable method in the diagnosis of the aujeszky's disease virus, the antigen employed being a cell culture virus. a virulent virus, a slightly virulent one, and the vaccinal mutant strain mk have shown a good complement-fixing activity and they detect specific complement-fixing antibodies in the immune serum. by means of cross cft an antigenic relation has been established between some strains of the herpes group and the aujeszky's ... | 1977 | 199985 |
| [pathogenesis of aujeszky's disease]. | results are presented of studies on the various localization of the virus of aujeszky's disease (the avirulent mutant strain mk and the virulent strain 2) in the organism of experimentally infected pigs. at the oral infection of pigs with strain mk the virus has been isolated from the submandibular lymph nodes of the animals only, the pigs being killed on the second and fifth day following infection with a low plaque-forming titer. however, from pigs that have been infected subcutaneously no vir ... | 1977 | 199987 |
| comparative analyses of the proteins and antigens of five herpesviruses. | | 1977 | 200709 |
| the effect of antibody on latent pseudorabies virus infection in vitro. | cytosine arabinoside (ara c) inhibited the synthesis of pseudorabies virus when vero cells were infected at a multiplicity of infection of 0.0001-0.05 pfu per cell. on removal of ara c, infectious virus reappeared after a latent period of 3-5 days. the activation of latent virus was not influenced by elevating the temperature to 40 degrees c at the time of ara c removal but it was prevented by antiviral antibody. when antiviral igg was added into the culture fluid of cells either during the incu ... | 1979 | 44663 |
| susceptibility of bovine macrophage and tracheal-ring cultures to bovine viruses. | cultures of macrophages initiated from peripheral blood monocytes and organ cultures of tracheal rings were tested for their susceptibility to bovine viruses. with several notable exceptions, viruses cytopathogenic for bovine embryonic lung cultures were cytopathogenic for macrophages. although cowpox virus replicated in macrophages, pseudocowpox did not, and although pseudorabies virus replicated within macrophages, infectious bovine rhinotracheitis and dn-599 herpesviruses did not. bluetongue ... | 1977 | 201195 |
| presence of aujeszky's disease virus in organs and materials from experimentally infected dogs. | the presence of aujeszky's disease virus was investigated in various organs and materials from experimentally infected dogs, employing the fluorescent antibody test (fat), tissue culture and biological tests in rabbits. the fat appeared less sensitive for virus detection than virus isolation in cell culture and rabbit tests. the virus distribution in the central nervous system was related to the site of inoculation. | 1977 | 201547 |
| attempts to immunize monkeys against experimental infection with herpesvirus suis. | | 1977 | 201677 |
| [use of the plaque method for isolating aujeszky's disease virus from meat, organs and meat products]. | comparative virologic studies were carried out to isolate the virus of aujesky's disease from meat, viscera, and raw smoked products by the plaque method as modified by sullivan and read as well as by the method with which the presence of the virus is recorded visually by the cytopathic effect observed in the infected cell monolayer. it was found that at the veterinary and sanitary inspection of meat and meat products produced from diseases or survived animals the modified plaque method proved m ... | 1977 | 202066 |
| comparative susceptibility of rabbits, rats, mice and pigs to infection with aujeszky diseases virus (adv) in the development of an efficacy test for adv vaccines. | | 1977 | 202116 |
| viral superinfection in cells carrying an arenavirus and/or a togavirus. | four lines of the same l-cell clone were transferred 60 times in parallel: uninfected cells, a line carrying lymphocytic choriomeningitis virus (lcmv), another one carrying tick-borne encephalitis virus (tev) and one carrying both viruses. in double persistency, lcm and tev were suppressed and stimulated, respectively. cell multiplication rates were comparable in all four lines. single lcmv persistence caused marked resistance of l cells to superinfecting viruses from various taxonomic groups, b ... | 1978 | 35946 |
| influence of deae dextran upon pig lymphocyte stimulation by aujeszky disease virus antigen. | | 1977 | 203145 |
| latent pseudorabies virus infection established at supraoptimal temperature. | little or no infectious virus was recovered from bhk-21 cells adapted to 40 degrees c, when they were infected with an attenuated strain of pseudorabies virus (prv) at low multiplicity of infection (moi) and subsequently kept at 40 degrees c. by passaging the infected cells at 40 degrees c, infectious virus disappeared within 2-3 passages. when the cells were shifted down to 37 degrees c, activation of virus growth occurred after a latent period of 48-72 hr. infected cells kept at 40 degrees c w ... | 1978 | 35944 |
| pseudorabies virus associated with abortion in swine. | | 1978 | 203566 |
| infectious agents associated with fetal and early neonatal death and abortion in swine. | laboratory examination of specimens from 824 porcine abortions over a 6-year period produced 320 (38.8%) diagnoses. viruses were involved in 22% and bacteria in 16.5% of all cases. enterovirus was the virus isolated most often (10.9%), followed by parvovirus (4.9%), reovirus (4.4%), pseudorabies virus (1%), and adenovirus (0.8%). leptospirosis was the bacterial infection most commonly diagnosed (9.8%), with numerous miscellaneous bacterial and fungal infections making up the remainder (7.0%) of ... | 1978 | 203567 |
| [potential virostatics. 1. quinoxalines]. | | 1977 | 203954 |
| the effect of supraoptimal temperature on the formation of pseudorabies virus particles. | replication of virulent and attenuated strains of pseudorabies virus (prv) at supraoptimal temperature was inhibited in l cells non-adapted to 40 degrees c. electron microscopy revealed the prevalence of hollow core particles without nucleoid; both the nuclei and cytoplasm of l cells were markedly altered at supraoptimal temperature. in bhk-21 cells adapted to 40 degrees c only replication of attenuated strains was inhibited whereas the virulent ones reached high titres at this temperature. the ... | 1977 | 23664 |
| [combined action of dna-synthesis inhibitors on the reproduction of dna viruses]. | | 1977 | 204162 |
| development and evaluation of a microimmunodiffusion test for detection of antibodies to pseudorabies virus in swine serum. | a microimmunodiffusion test (midt) was developed for the detection of pseudorabies virus (prv) antibodies in swine serum. the optimal medium for the midt was determined to contain 0.69% agarose in 0.05 m tris buffer (ph 7.2) with 0.025% sodium azide and no nacl. the prv antigen prepared by (nh4)2so4 precipitation of viral fluids (42.5 g/100 ml), dialyzed against distilled water, and concentrated to approximately 100-fold of the original volume with polyethylene glycol (mol wt 20,000) provided a ... | 1978 | 204231 |
| the effect of hypertonic conditions on protein synthesis in cells infected with herpes virus. | | 1978 | 204525 |