| search for herpetic antibodies in the cerebrospinal fluid in senile dementia and mental retardation. | complement-requiring neutralizing antibodies to herpes simplex type 1 virus (hsv 1) in titres from 2 to greater than 16 were detected in the cerebrospinal fluid (csf) of 47% senile patients with various forms of dementia, but in none of mentally retarded adolescents and adults suffering from various neurologidal diseases. also the incidence of hsv 1 serum antibodies in elevated titres (larger than or equal to 512) was increased in senile demented patients (61%) as compared with persons in normal ... | 1975 | 1996 |
| reactions of immune sera against the nucleocapsid, envelope and whole herpes simplex virus type 1. | antibodies against (a) naked particles, (b) virus envelope, and (c) whole herpes simplex virus (hsv) type 1 were investigated. immune rabbit serum against naked particles (and formalized naked particles) contained virus neutralizing (vn) antibody in a low titre as compared to the titre of complement-fixing (cf) antibody. immune rabbit serum against the viral envelope had similar titres of vn and cf antibodies. in gel double diffusion precipitation tests, whole hsv gave two precipitation zones wi ... | 1976 | 7940 |
| measurement of antibodies to herpesvirus types 1 and 2 in human sera by microradioimmunoassay. | the binding of 125i-labelled anti-human antibodies against the fc igg fragment to unlabelled antiviral immunoglobulins in the surface of infected cells was used to quantitate antibodies against herpes simplex virus type (hsv-1) and type 2 (hsv-2) in sera from patients with cervix carcinoma. the microradioimmunoassay technique (micro-ria) proved to be 5-10 times more sensitive than the microneutralization test. antibody titres determined by micro-ria correlated with neutralizing antibody titres t ... | 1977 | 22235 |
| experimental pathogenesis of non-lethal herpesvirus infection and the establishment of latency. | in rabbits inoculated into the right scarified cornea with the kupka strain of human herpesvirus type 1 (hhv 1), healing of the keratitis occurred between days 8--12 post infection (p.i.). viral particles were seen in nuclei and cytoplasm of corneal epithelium cells and in a few fibrocytes of corneal stroma. by immunofluorescence and electron microscopy, the virus was demonstrated in single neurons and satellite cells of the homolateral gasserian ganglion since 24 hr p.i. from 20 hr to 8 days p. ... | 1978 | 29466 |
| differential feulgen-deoxyribonucleic acid hydrolysis patterns of herpes simplex virus type 1 and type 2 infected cells. | infection of human embryonic lung cells with herpes simplex virus type 1 (hsv-1) and herpes simplex type 1 (hsv-2) resulted in: (a) qualitative (nuclear cytopathologic) alterations and quantitative (nuclear area) differences in infected compared to control nuclei; (b) increased feulgen-deoxyribonucleic acid (f-dna) amounts in infected cells, probably due to viral dna; (c) higher f-dna levels in hsv-2 infected cells; and (d) increased rates of f-dna hydrolysis in viral-infected as compared to uni ... | 1975 | 47871 |
| a study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens. | infectious adenovirus type 5 and coxsackievirus type b5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees c for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen preparations with serial twofold dilutions of human antisera and fluorescein-labeled goat anti-human immunoglobulin g. lipid-containing herpes simplex virus type 1 and respiratory s ... | 1976 | 61971 |
| glycoproteins specified by herpes simplex virus type 1: their synthesis, processing and antigenic relatedness to hsv -2 glycoproteins. | | 1975 | 65316 |
| cell-mediated immunity to herpes simplex virus types 1 and 2 antigens in leukoplakia and carcinoma in man. | cell-mediated immune responses to herpes simplex virus 1 and type 2 virion and non-virion antigens were assessed in patients and in controls with oral leukoplakia, carcinoma and recurrent herpes labialis. enhanced proliferation was found in lymphocytes from patients with recurrent herpes labialis or with leukoplakia showing epithelial atypia, and depressed responses were found in carcinoma. very significantly positive correlations were shown between the responses to each of the herpes virus anti ... | 1976 | 65753 |
| an immunofluorescence technique with counterstain on fixed cells for the detection of antibodies to human herpesviruses; antibody patterns in patients with hodgkin's disease and nasopharyngeal carcinoma. | an indirect immunofluorescence (if) test on fixed cells with evans' blue counterstain is described for all four human herpesviruses, i.e., herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2), varicella-zoster virus (vzv), cytomegalovirus (cmv) and epstein-barr virus (ebv). comparison with immunodiffusion (id) for hsv-2 and with id and complement fixation (cf) for vzv and cmv demonstrated the specificity and high sensitivity of the if test. also introduced is a modification of the anti-compl ... | 1976 | 67099 |
| enhancement of ige-mediated histamine release from human basophils by viruses: role of interferon. | human leukocytes maintained in culture are induced to release histamine when exposed to ragweed antigen e or anti-ige. leukocyte cultures incubated with virus (i.e. hsv-1, influenza a, and adeno-1) but not exposed to ragweed antigen e or anti-ige fail to release histamine. if, however, leukocyte cultures are first exposed to virus and then to ragweed antigen e or anti-ige, significant enhancement of histamine release occurs. both infectious and inactivated virus enhance histamine release and the ... | 1977 | 67173 |
| detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their specificity. | skin fibroblasts exposed to cytosine arabinoside (ara c) were infected with either cytomegalovirus (cmv) or herpes simplex virus (hsv) type 1 and 2. herpesvirus-determined early antigens (hv-ea), detected by anti-complement immunofluorescence (acif), occurred primarily in the nucleic, and the specificity of these results was established by an acif blocking reaction using f(ab')2 fragments of human and hyperimmune reference sera. direct tests with selected sera and cross-blocking experiments betw ... | 1977 | 64461 |
| quantitation of antibodies to herpes simplex virus types 1 and 2 by complement-dependent antibody lysis of infected cells. | the release of 51cr from cells infected with herpes simplex virus type 1 or type 2 by antibody and complement was examined as a method of quantitating antibodies to the viruses. with decreasing concentration of antibody, a typical dose-response curve was observed; a region of antibody excess in which dilution did not affect percentage of specific 51cr release followed by a region in which a linear relation existed between dilution and percentage of specific 51cr release. therefore, a quantitativ ... | 1976 | 60057 |
| basophil-sensitizing antibody response to herpes simplex viruses in rabbits. | antibodies to herpes simplex virus type 1 and type 2 were detected in the sera of rabbits by release of histamine from basophils sensitized in vitro with the sera. the time course of the appearance of the antibodies, the dose-response curve of the release of histamine in relation to antigen concentration, the sedimentation characteristics of the antibodies in sucrose gradients, and the ability to destroy the sensitizing capacity of the sera with heat suggest that the antibodies being assessed we ... | 1976 | 58935 |
| a rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology. | a rapid simplified technique, based on restriction-endonuclease analysis of radioactively labelled d.n.a. is described; it distinguishes herpes-simplex virus type 1 strains from herpes-simplex virus type 2 unambiguously. large numbers of isolates can be screened in 4--5 days. | 1979 | 86095 |
| herpes simplex virus type 1 replication in a gingival cell line. | propagation of herpes simplex virus type 1 (hsv-1) in gingival cells is advantageous since these cells are more definitive than hela cells in demonstrating viral-induced cytopathic effects and are easily and economically maintained in the laboratory. they also provide a potential medium for studying oral tissue reactions to hsv-1 infection. hsv-1-induced cytopathic effects are clearly characterized following inoculation with virus from standard laboratory cultures and human infections. | 1975 | 49366 |
| apparent absence of virus-specific transplantation rejection antigen in herpes simplex virus-transformed hamster cells. | hsv-1 sensitized hosts were not resistant to challenge with small numbers of hsv-1 transformed cells. virus immunization did not inhibit or enhance the incidence of tumour metastases. | 1978 | 87369 |
| differentiation of herpes simplex virus type 1 and type 2 by immunofluorescence: discriminative staining by labelled igg preparations. | while evaluating herpes simplex virus (hsv) typing by indirect immunofluorescence staining, an undesired specific staining pattern turned out to be a reliable marker for herpes simplex type 1. cells infected with herpes simplex type 1 displayed clear staining with a fitc-conjugated antiglobulin preparation, also in the absence of herpes simplex-specific antibodies. using the same conjugate, herpes simplex type-2-infected cells exhibited no fluorescence. the particular type of staining observed w ... | 1979 | 89761 |
| lifetimes of mrna molecules directing the synthesis of viral proteins in herpes simplex virus-infected cells. | possible variations in the functional lifetimes of herpes simplex virus type 1 mrna molecules in infected hela cells were studied. as shown by the rate of decrease of radioactive amino acid incorporation into viral proteins after the addition of actinomycin, the average lifetime of early viral mrna's are shorter than those for the late messenger species. in addition, when the viral proteins made after the addition of actinomycin were further analyzed by gel electrophoresis, it was found that mes ... | 1975 | 46283 |
| detection of herpes-simplex viral genome in brain tissue. | herpes-simplex virus type (hsv-1) nucleic-acid sequences were detected by in-situ hybridisation in thin sections of brains from mice which had been inoculated 24 weeks previously with hsv-1. these mice were not ill, and infectious virus could not be recovered from them. hsv-1 sequences were also present in brain smears from 3 of 4 elderly patients who had died with chronic psychiatric illness and neuropathological changes but not in smears from 2 patients who had had acute psychotic episodes and ... | 1979 | 90272 |
| effect of cytosine arabinoside on viral-specific protein synthesis in cells infected with herpes simplex virus. | the relationship between viral dna and protein synthesis during herpes simplex virus type 1 (hsv-1) replication in hela cells was examined. treatment of infected cells with cytosine arabinoside (ara-c), which inhibited the synthesis of hsv-1 dna beyond the level of detection, markedly affected the types and amounts of viral proteins made in the infected cell. although early hsv-1 proteins were synthesized normally, there was a rapid decline in total viral protein synthesis beginning 3 to 4 h aft ... | 1975 | 46282 |
| effect of 2-deoxy-d-glucose on the binding of preimmune and immune sera to herpes simplex virus-infected cells as measured by quantitative immunofluorescence. | the binding of immune and preimmune rabbit sera to fixed vero cells infected with herpes simplex virus type 1 (hsv 1) was measured by quantitative immunofluorescence. false positive binding of the preimmune serum (ps) occurred from 8 hr post infection (p.i.), while the immune serum (is) revealed positive fluorescence starting with 4 hr p.i. the false binding of ps decreased significantly (p = 0.01) if the cells were cultured in the presence of 2-deoxy-d-glucose (dog). statistical analysis of dat ... | 1979 | 42300 |
| immunoelectron microscopic localization of herpes simplex virus antigens in infected cells using the unlabeled antibody-enzyme method. | subcellular localization of viral antigens was demonstrated during viral morphogenesis using herpes simplex virus type 1 (hsv-1) infected monolayers of rabbit cornea cells. the localization was done by immunoelectron microscopy employing the peroxidase-antiperoxidase (pap) immunocytochemical technique and the postembedding staining method. the localization of viral antigens was followed at time intervals during infection from 2 to 19 hr. after exposure of sections to either polyspecific antibodi ... | 1979 | 92500 |
| detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay. | a micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the test is performed by coating wells of microtiter plates with purified hsv-specified glycoproteins, vp123 and vp119, derived from hsv-1- and hsv-2-infected cells, respectively. the coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human igg reagent. because these m ... | 1979 | 94044 |
| detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the papanicolaou stain. | the sensitive peroxidase-antiperoxidase (pxapx) method individually and in conjunction with the papanicolaou (pap) stain was used to detect herpes simplex virus (hsv) in specimens from human female genitalia. initial studies using a model system of hsv-1 or hsv-2-infected vero cells established (i) acetone as the most effective fixative, (ii) optimal dilutions of preimmunization and anti-hsv serum for differentiation of infected from noninfected cells, (iii) optimal concentration of 3,3'-diamino ... | 1979 | 94600 |
| influence of human leukocyte interferon on igg on the replication of herpes simplex virus in nervous tissue in vitro. | herpes simplex virus type 1 (hsv-1) replicated productively in rabbit and guinea pig ganglia and nerve organ cultures when inoculated in high titres. treatment with igg 20 hr before and 48 hr after infection produced a delay of 4 to 7 days in the recovery of hsv-1 by the method of co-cultivation. the same result was obtained when igg was combined with human leukocyte interferon. there was no difference in the period up to hsv recovery between the groups treated with interferon alone and the hsv ... | 1979 | 94767 |
| analysis of herpes simplex virus isolated from patients with recurrent herpes keratitis exhibiting "treatment-resistance" to 5-iodo-2'-deoxyuridine. | four strains of herpes simplex virus (hsv) were isolated from two patients with recurrent herpes keratitis who failed to respond to 5'-iodo-2'-deoxyuridine (idu) treatment. two of the four isolates were highly resistant to idu in cell culture and the other two isolates were more susceptible to idu than an hsv-1 laboratory strain. from each patient, an idu-resistant and an idu-susceptible virus was isolated. all 4 isolates possessed the ability to induce the thymidine kinase (tk) activity in cell ... | 1979 | 41435 |
| photosensitization of herpes simplex virus type 1 with neutral red. | commercial neutral red (nr) originally containing at least 8 components was purified by thin layer chromatography. herpes simplex virus type 1 (hsv-1) treated in vitro with 30 microgram/ml of purified nr became sensitive to light inactivation within 2 min but rapidly lost this sensitivity upon dilution. similarly, virus grown in the presence of nr lost its photosensitivity upon dilution of the virus stock. in both cases the kinetics of inactivation appeared to be multi-hit. photoinactivation of ... | 1978 | 31414 |
| failure of 5-bromodeoxyuridine to influence the activation of latent herpex simplex virus infection in explants of the rabbit regional sensory ganglia. | the occurence of virus-specific antigens in explants of the gasserian ganglia of rabbits with healed keratitis induced by the kupka strain of human herpesvirus type 1 was tested by indirect immunofluorescence. in explants, the neurons and satellite cells revealed specific fluorescence from the 3rd day of culturing. the pattern of virus activation in the ganglion tissue was similar irrespective of whether the explants were cultured in the presence or absence of 5-bromo-2'-deoxyuridine. | 1978 | 27976 |
| nonstructural proteins of herpes simplex virus. i. purification of the induced dna polymerase. | herpes simplex virus-induced dna polymerase purified by published methods was found to be contaminated with many others proteins, including virus structural proteins. thus, deae-cellulose and phosphocellulose chromatography were used in combination with affinity chromatography to purify dna polymerase from herpes simplex virus type 1- and type 2-infected cells. the purified enzyme retained unique features of the herpesvirus-induced dna polymerase, including a requirement for high salt concentrat ... | 1977 | 21304 |
| syntheses of virus-induced thymidine kinase and viral dna in herpes simplex type 1 virus-infected chick embryo fibroblasts. | herpes simplex virus type 1, strain kupka, did not replicate in chick embryo fibroblasts (cef), but the infection was followed by the development of cytopathic changes. this effect could be abolished by uv irradiation of the virus. virus-induced thymidine kinase was synthesized in the infected cells reaching a maximum level at 24 hours post infection (p.i.). in the presence of cytosine arabinoside, thymidine kinase synthesis was enhanced. this suggested that the late (post-replicative) viral fun ... | 1977 | 18914 |
| ribonucleotide reductase from herpes simplex virus (types 1 and 2) infected and uninfected kb cells: properties of the partially purified enzymes. | mammalian ribonucleotide reductase is a complex enzyme modified in its activity by a complex regulatory system involving adenosine triphosphate (atp) and deoxyribonucleoside triphosphates. infection of kb cells with herpes simplex virus (hsv) type 1 or 2 induces the formation of an altered ribonucleotide reductase. the properties of partially purified reductase from uninfected kb cells have been compared with the enzymes obtained from hsv-1 and hsv-2 infected kb cells. we found that the virus-in ... | 1977 | 18550 |
| genetic studies with herpes simplex virus type 1: quantitative analysis of the products from two-factor crosses. | | 1975 | 163543 |
| ph mediated inhibition of the cell to cell spread of herpes simplex virus infection. | the relationships between the environmental ph and the replication and spread of herps simplex virus (hsv) infection in rabbit skin (rs) cell cultures was studies. relative to the plaque formation at ph7.0, incubation of rs cells with medium adjusted to ph 6.6 resulted in a 50 to 70 per cent decrement in plaque number. the addition of overlay media adjusted to ph 6.0 or 6.3 precluded hsv plaque formation. select hsv-1 (type 1) and hsv (type 2) strains readily survived a 3 day incubation with med ... | 1976 | 11763 |
| stimulation of human lymphocytes by herpes simplex virus antigens. | lymphocytes from individuals with laboratory evidence of prior infection with herpes simplex virus (hsv) type 1 or type 2 demonstrated transformation (av antigens. higher stimulation indexes were obtained when lymphocytes were incubated with the homologous as compared with the heterologous antigen. higher mean lymphocyte stimulation indexes were also demonstrated in seropositive as compared with seronegative individuals. lymphocytes from children with hsv-1 stomatitis usually became responsive t ... | 1975 | 163788 |
| immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture. | proteins released from herpes simplex virus type 1 (hsv-1)-and type 2 (hsv-2)-infected hep-2 cells have been characterized by the crossed immunoelectrophoretic technique. both hsv type-common and type-specific antigens were found in the tissue culture medium 24 h after infection. antigen ag-6, an hsv-1-specific antigen, was found in high concentration in the medium as compared to other hsv antigens released from hsv-1-infected cells. the hsv-2-specific antigens, ag-4 and ag-1, were released in m ... | 1979 | 85610 |
| iontophoretic application of adenine arabinoside monophosphate to herpes simplex virus type 1-infected hairless mouse skin. | several antiviral agents were applied topically or by iontophoresis to hairless mouse skin inoculated with herpes simplex virus type 1 (hsv-1), and the chemotherapeutic effectiveness was evaluated. topical application of iododeoxyuridine, arabinoside a, and adenine arabinoside monophosphate (ara-amp) moderately decreased the average lesion score, number of mice with paralysis, and number of mice dying in hsv-1-infected animals. also, the mean survival time was moderately prolonged by topical app ... | 1978 | 82423 |
| crossed immunoelectrophoresis of a herpes simplex virus type 1-specific antigen: immunological and biochemical characterization. | crossed immunoelectrophoresis of herpes simplex virus (hsv) antigens obtained from infected cells solubilized with the nonionic detergent triton x-100 has shown that antigen number 6 (ag-6) is found in preparations from hsv type 1-infected cells but not in hsv type 2 preparations. a monospecific antiserum was produced against ag-6 by intracutaneous inoculation of the corresponding immunoprecipitate into rabbits. this antiserum neutralized hsv type 1 but not hsv type 2. the polypeptide compositio ... | 1978 | 81867 |
| type-common cp-1 antigen of herpes simplex virus is associated with a 59,000-molecular-weight envelope glycoprotein. | the cp-1 antigen of herpes simplex virus type 1 (hsv-1) is a glycoprotein found in the soluble portion of infected cells, in detergent extracts of infected cell membranes, and in the envelope of purified virus. antisera were prepared against a further purified form of cp-1 prepared from hsv soluble antigen mix; a glycoprotein, gp52, isolated from detergent-treated infected cells; and detergent extracts of purified virus. each of the antisera reacted with cp-1 to give a single immunoprecipitin ba ... | 1978 | 80458 |
| cellular immune responses to herpes simplex virus type 1 in recurrent herpes labialis: in vitro blastogenesis and cytotoxicity to infected cell line. | cellular immunity to herpes simplex virus type 1 (hsv-1) in 12 volunteers with recurrent herpes labialis was evaluated by means of two microassays. in the blastogenesis assay, lymphocytes were incubated with tissue culture cells persistently infected with hsv-1. uninfected cells were used as controls, and a blastogenic index was calculated. the mean blastogenic index (plus or minus sd) for subjects with recurrent herpes labialis was 26.9 (plus or minus 8.3); the mean blastogenic index (plus or m ... | 1975 | 165245 |
| organ culture of human aorta: prolonged survival with support of viral replication. | organ cultures were established with use of human fetal aorta obtained after death; cellular elements were preserved in the cultures for periods of up to eight weeks, with maintenance of their in vivo tissue relationships. histologically, these cultures preserve the three coats of the aorta, including an intact endothelial layer cells. the aorta organ cultures supported the replication of echovirus 11, herpes simplex virus type 1, adenovirus 2, and coxsackievirus b5 without the production of det ... | 1975 | 165246 |
| possible role of fc receptors on cells infected and transformed by herpesvirus: escape from immune cytolysis. | receptors for the fc portion of nonimmune immunoglobulin g were demonstrated on b103 rat brain neuroma cells infected with herpes simplex virus type 1 (hsv-1) kos by a radioimmunoassay using 125i-labeled heat-aggregated fc fragments. immune f(ab')2 fragments specific for hsv antigens competed efficiently for fc binding sites, suggesting that the binding of fc fragments to infected cells is specific for viral cell-surface antigens. it has been suggested that the binding of immune complexes to fc ... | 1978 | 80379 |
| identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and b virus. | in immunoelectrophoretic analyses one common antigen was demonstrated in antigen preparations from herpes simplex virus types 1- and 2- (hsv-1 and hsv-2), bovine herpes mammillitis (bhm) virus-, and b virus-infected cells solubilized by triton x-100. the antigen was also demonstrated in solubilized purified hsv-1 and bhm virus. the common antigen was identified as antigen 11 of hsv-1 or hsv-2. differences were found in the polypeptide composition of the related antigens when isolated from the fo ... | 1978 | 78988 |
| determination of virus-specific antigens in extracts from herpes simplex virus-infected cells by a cr release inhibition test. | a 51cr release inhibition test (crit) was developed for determining herpes simplex virus type 1 antigens in infected cell extracts. among the factors studied were the optimal concentrations of complement, antibody, and infected target cells. under standardized conditions, the relationship between antigen content and inhibition of chromium release was linear in a semilogarithmic plot, indicating that the antigen content can be determined from testing two dilutions of a given preparation. other te ... | 1978 | 78899 |
| effect of human leukocyte interferon on vaccinia-and herpes virus-infected cell cultures and monkey corneas. | pretreatment of human fibroblast cultures with human leukocyte interferon (hif, 1,000 iu/ml) resulted in a 24-h delay of virus replication after infection with vaccinia virus and herpes simplex virus type 1 and type 2. additional hif treatment 24 h after infection effectively lowered the maximum yield of viral infectivity. equal results were obtained in simian cells with 3,000 iu of hif per ml. the spread of two cell-bound herpesviruses, varicella zoster virus and medical lake macaque herpesviru ... | 1975 | 166924 |
| transmission of herpes-simplex virus type 1 in a nursery for the newborn. identification of viral isolates by d.n.a. "fingerprinting". | the occurrence of herpes-simplex-virus type-1 infections in two newborn infants in a nursery within a one-month period suggested the possibility of transmission in the nursery. one infant may have been infected by his father, who had active herpes labialis at the time of the child's birth. the source of the second infant's infection was not apparent. viruses isolated from the two infants were "fingerprinted" by cleaving the virus-specific d.n.a. with several restriction endonucleases and compari ... | 1978 | 76893 |
| clinical evaluation of commercial conjugates for direct immunofluorescence of herpes simplex virus. | commercially prepared antibody conjugates that are now available allow extensive application of direct immunofluorescence for diagnosing herpetic infections. five of these conjugates--microbiological associates bivalent herpes antiserum (mba-bivalent), mba anti-herpes types 1 (mba-1) and 2 (mba-2), and flow laboratories anti-herpes types 1 (flow-1) and 2 (flow-2)--were compared for antibody titer, diagnostic accuracy, and nonspecific background staining on known infected tissue. four of these we ... | 1977 | 73546 |
| detection of herpes simplex virus-related antigens in the nuclei and cytoplasm of biochemically transformed cells with peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence. | herpes simplex virus (hsv)-related antigens have been demonstrated in the nuclei and cytoplasm of human and mouse cells biochemically transformed by ultraviolet light-irradiated hsv. this was accomplished by using peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence with rabbit antisera that had high neutralizing titers against the hsv-specific thymidine kinase activity and virus infectivity. hsv-1 antisera reacted with antigens in cells biochemically transformed by ... | 1977 | 71279 |
| cellular immune response to human cytomegalovirus. i. lymphocyte transformation studies in the rabbit. | the lymphocyte transformation assay was used to monitor the cellular immune response of rabbits sensitized to cytomegalovirus (cmv). peripheral blood lymphocytes from animals inoculated with purified virus were specifically stimulated by crude or twice-banded cmv. blood cells from rabbits immunized to herpes simplex virus type 1 (hsv-1) responded to that antigen but not to cmv. viable or heated cmv preparations stimulated unwashed blood cells as efficiently as washed cells. furthermore, preincub ... | 1979 | 85612 |
| isolation of a nucleocapsid polypeptide of herpes simplex virus types 1 and 2 possessing immunologically type-specific and cross-reactive determinants. | a polypeptide (p40) of approximately 40,000 molecular weight was isolated from herpes simplex virus type 1 and 2 nucleocapsids by gel filtration and ion exchange chromatography. this protein appears to be the same as protein 22a described previously (gibson and roizman, j. virol. 10:1044--1052, 1972). competition immunoassays were developed by using purified p40 and antisera prepared in guinea pigs. the assays indicated that the p40's from herpes simplex virus types 1 and 2 possess both type-spe ... | 1979 | 85720 |
| observations of antigenic relatedness between viruses of the herpes simplex "neutroseron". | the antigenic relatedness of three viruses of the herpes simplex type 1 neutroseron - herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) and bovine mammillitis virus (bmv) - has been examined by immune precipitation and virus neutralization tests. many virus-specific infected-cell polypeptides were shown to possess antigenic sites shared by both hsv-1 and hsv-2. cross-neutralization between the viruses is mediated through antibodies to at least two antigenic sites, one shared by hsv-1, hsv-2 and ... | 1978 | 87364 |
| effect of iontophoretic and topical application of antiviral agents in treatment of experimental hsv-1 keratitis in rabbits. | cathodal (-) iontophoresis of 9-beta-d-arabinofuranosyl-adenine 5'-monophosphate (vidarabine monophosphate; ara-amp) was performed once daily for 3 days for the treatment of experimental herpes simplex virus type 1 (hsv-1) keratitis in rabbit eyes, and the therapeutic efficacy was compared with that of topical treatment of ara-amp and idoxuridine (idu) administered five times daily for 4 days. with the treatment initiated 24 hr after viral inoculation, ara-amp cathodal iontophoresis resulted in ... | 1979 | 90030 |
| production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2. | we prepared mouse hybrid cell lines which produced antibodies against herpes simplex virus type 1 and 2 nucleocapsids. cell lines 1d4 and 3e1, respectively, secreted immunoglobulin g1 herpes simplex virus type 1 and immunoglobulin g1 herpes simplex virus type 2 antibodies which immunoprecipitated proteins designated p40 and p45 from homologous nucleocapsid preparations but precipitated no proteins from heterologous preparations. in contrast, guinea pig antisera prepared against either herpes sim ... | 1979 | 91687 |
| association between pre-transplant natural kill and graft-versus-host disease after stem-cell transplantation. | natural killer activity against herpes simplex virus type 1 infected fibroblasts nk(hsv-1) was studied prospectively in patients undergoing allogenic bone-marrow or fetal-tissue stem-cell transplantation. thirteen patients showed evidence of engraftment and survived long enough to develop graft-versus-host disease (gvhd). of this group, all of the seven having normal nk(hsv-1) activity before transplantation acquired gvhd and the six having low nk(hsv-1) had no evidence of gvhd. these results we ... | 1979 | 91840 |
| anatomy of herpes simplex virus dna: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. | digestion of herpes simplex virus dna by the hiniii or eco ri restriction endonucleases yielded 11 to 15 fragments with molecular weights between 1 x 10(6) and 28 x10(6). the electrophoretic profiles obtained in 0.3% agarose gels with dna fragments from none different strains of herpes simplex virus type 1 could be readily differentiated from the patterns exhibited by the corresponding fragments from four separate strains of type 2 virus; however, with each serotype, the laboratory strains diffe ... | 1975 | 168574 |
| immunological nature of genetic resistance of mice to herpes simplex virus type 1 infection. | treatment of mice genetically resistant to hsv-1 with agents which impair t-cell or macrophage function markedly diminished resistance. bone marrow transplantation of genetically susceptible mice with marrow from resistant f1 mice produced chimeras resistant to hsv-1. resistance thus appears to be immunological in nature. striking similarities were found between resistance to hsv-1 and allogeneic resistance. | 1978 | 109389 |
| invasion of the central nervous system by herpes simplex virus type 1 after subcutaneous inoculation of immunosuppressed mice. | young adult mice given subcutaneous inoculations in the rear footpad with herpes simplex virus type 1 were found to be resistant to infection unless they were immunosuppressed with sublethal doses of irradiation or cyclophosphamide. in these mice, virus multiplied in the sciatic nerve three to four days after inoculation. this development was followed by multiplication of virus in the spinal cord and brain, which caused death of the mice in about eight days. no virus was detected in the blood or ... | 1975 | 162925 |
| cell-mediated immunity to herpes simplex in humans: lymphocyte cytotoxicity measured by 51-cr release from infected cells. | we assessed cell-mediated immunity to herpes simplex virus type 1 antigen in patients suffering from recurrent cold sores and in a series of healthy controls. paradoxically, all those subject to recurrent herpetic infections had, without exception, evidence of cell-mediated immunity to herpes antigens. this was demonstrated by lymphocyte transformation and specific 51cr release from infected human amnion cells after incubation with peripheal blood mononuclear cells. where performed, skin tests w ... | 1975 | 163220 |
| potentiation of an adenovirus-associated virus by herpes simplex virus type-2-transformed cells. | the herpes simplex virus type-2 (hsv-2)-transformed cell line 333-8-9 was inoculated with adeno-virus-associated virus type 3 (aav-3) and examined by immunofluorescent (fa) techniques for evidence of aav-3 antigen synthesis. antisera prepared against the three sodium dodecyl sulfate-dissociated structural polypeptides (vp-1, vp-2, and vp-3) of aav-3 and against the whole aav-3 virion were used as probes to search for aav-3 fa-stainable antigens synthesized by these cells. the 333-8-9 cells inocu ... | 1975 | 163319 |
| chromosome aberrations in syrian hamster embryo cells transformed after exposure to ultraviolet-irradiated herpes simplex virus type 1 or 2. | six syrian hamster embryo cell lines (14-012-8-1, kos-6-1, 333-8-9, 333-2-29, ms-4-1, fr-6-1), developed after exposure of primary cultures to different strains of uv-irradiated herpes simplex virus (hsv) type 1 or 2, were analyzed for chromosome aberrations. all the cell lines showed chromosome stability (number of chromosomes were maintained within a narrow range of variation in the diploid region) and a low incidence of polyploids, endoreduplications, and metaphases with pulveration or extens ... | 1975 | 163328 |
| quantitative assay for transformation of 3t3 cells by herpes simplex virus type 2. | the interaction of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) with swiss/3t3 cells was investigated. virus-induced cytopathic effects developed in the absence of production of infectious virus. hsv-2 inactivated with uv light (2, 4, 6, and 8 min) also induced cell death in the absence of virus replication. cell death was not detectable after infection by hsv-2 that had been inactivated by uv irradiation for 10, 12, and 14 min. 3t3 cells infected with uv-inactivated virus (10 and 12 m ... | 1975 | 163369 |
| increased susceptibility to infection with herpes simplex virus types 1 and 2 of cold-adapted l cells. | l cells (l-as subline) have been adapted to a temperature of 4 degrees c. in the cold-adapted cells, designated lc3, greater amounts of infectious herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) were synthesized than in the original l-as cells or in another control l-cell line. two strains of hsv-1 reached higher infectious titres in lc3 cells grown at 36 degrees c than in those grown at 32 degrees c. the hsv-2 strain tested replicated in lc-3 cells grown at 32 degrees c better than at higher ... | 1978 | 26196 |
| biological effects of 5-carboxy-2'-deoxyuridine: hydrolysis product of 5-trifluoromethyl-2'-deoxyuridine. | 5-carboxy-2'-deoxyuridine (5-cooh-2'-durd) is a product of the base-catalyzed hydrolysis of 5-trifluoromethyl-2'-deoxyuridine. hydrolysis of 5-trifluoromethyl-2'-deoxyuridine to 5-cooh-2'-durd in phosphate-buffered saline was kinetically first order and was ph dependent. at 37 degrees c and ph 7.0, 7.5, and 8.0, hydrolysis occurred with rate constants of 4.19 x 10(-5), 9.30 x 10(-5), and 1.61 x 10(-4) s(-1), respectively, with corresponding half-lives of 45.7, 20.6, and 11.9 h. 5-cooh-2'-durd in ... | 1978 | 28691 |
| detection of a virus-specific antigen on the surface of herpes simplex virus-transformed cells. | a virion-associated antigen, cp-1, was detected on the surface of hamster cells after transformation by inactivated herpes simplex virus type 1. presence of the cp-1 antigen on the cells correlates with the synthesis of antibody by tumorbearing hamsters capable of neutralizing both herpes simplex virus types 1 and 2. | 1975 | 163379 |
| proteins specified by herpes simplex virus. xiii. glycosylation of viral polypeptides. | in the course of herpes simplex virus 1 (hsv-1) replication in human epidermoid carcinoma no. 2 cells, the synthesis and glycosylation of host cell proteins ceases and is replaced by the synthesis and glycosylation of virus-specified polypeptides. analyses of the synthesis of viral glycoproteins show that the glycosylation of viral polypeptides occurs late in the virus growth cycle and that certain of the precursors to major vital glycoproteins are members of the gamma group of polypeptides, i.e ... | 1975 | 171454 |
| comparison of the virion proteins specified by herpes simplex virus types 1 and 2. | purified herpes simplex virus type 2 (hsv-2) virions were found to contain approximately the same number of polypeptides as hsv type 1 (hsv-1) virions. comparisons of the structural proteins specified by five independent hsv-2 isolates revealed some minor differences in their electrophoretic profiles on sodium dodecyl sulfate-acrylamide gels; certain invariant features of the electrophoretic profiles, however, allowed clear differentiation between all the hsv-2 isolates and hsv-1. | 1975 | 171455 |
| herpes simplex virus microneutralization: a simplification of the test. | the herpes simplex virus (hsv) microneutralization test has been simplified; its use has been demonstrated for the identification of hsv isolates as type 1 (hsv-1) or type 2 (hsv-2) and for the measurement of antibodies to hsv-1 and hsv-2. in this test, the relation between the neutralization titer and virus input is linear, and thus test results can be expressed as corrected neutralization titers, rather than as the more complex neutralizing potency values previously proposed. by means of this ... | 1975 | 163870 |
| solid phase radioimmunoassay for typing herpes simplex viral antibodies in human sera. | an indirect solid phase radioimmunoassay (ria) was developed for typing antibody to herpes simplex virus (hsv) types 1 and 2 (hsv-1 and hsv-2) in human sera. the test is based upon absorption of sera with uninfected, hsv-1-infected cells and testing for residual antibody. the high sensitivity of the ria method for detecting hsv antibody permits examination of sera at high dilutions, and thus relatively small volumes of virus-infected cells are required for cross-absorption of antibodies. results ... | 1975 | 172526 |
| differential action of deoxynucleosides on mammalian cell cultures infected with herpes simplex virus types 1 and 2. | cytopathic effects induced by eight serologically defined isolates of herpes simplex virus type 2 (tested on human amnion cells) were markedly inhibited by thymidine at a concentration of 5 mm; eight serologically defined isolates of herpes simplex virus type 1, however, were not significantly inhibited. a similar effect was seen with thymidine, deoxyguanosine, and deoxycytidine at 1-mm concentrations in tests with rabbit kidney cultures. the inhibitory effect of thymidine was not blocked by the ... | 1975 | 163871 |
| demonstration of oncogenic potential of mammalian cells transformed by dna-containing viruses following photodynamic inactivation. | the oncogenic properties of hamster embryo cells transformed by herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and sv40 virus following photodynamic inactivation using neutral red were determined by subcutaneous inoculation into newborn syrian hamsters. cells transformed by all three viruses produced palpable tumors after different latent periods. histopathological examination showed that hsv-2 tumors were fibrosarcomas and metastases were often seen in the lungs. hsv-2 primary tumors we ... | 1975 | 165153 |
| antiviral activity of an adenosine deaminase inhibitor: decreased replication of herpes simplex virus. | a unique seven-membered heterocyclic-ring inhibitor of adenosine deaminase was studied. one preparation of the compound inhibited replication of herpes simplex virus in the absence of adenine arabinoside. in this capacity, the minimal inhibitory concentration of deaminase inhibitor for herpes simplex virus type 1 (hsv-1), with 50 percent reduction of plaque-forming units as the end point, was 37.7 mug/ml. this activity compared favorably with the inhibitory activity of ara-hypoxanthine (34.1 mug ... | 1975 | 166117 |
| anatomy of herpes simplex virus dna. iii. characterization of defective dna molecules and biological properties of virus populations containing them. | we have characterized the virus progeny and its dna from plaque-purified and undiluted passages of herpes simplex virus 1 in hep-2 cells. secifically, (i) infectious virus yields declined progressively in passages 1 through 10 and gradually increased at passages 11 through 14. the yields correlated with pfu/particle ratios. (ii) in cells infected with virus from passages 6 through 10, there was an overproduction of an early viral polypeptide (no. 4) and a delay in the synthesis of late viral pro ... | 1975 | 166202 |
| antibody-dependent cell-mediated cytotoxicity to target cells infected with type 1 and type 2 herpes simplex virus. | the phenomenon of antibody-dependent cell-mediated cytoxicity (adcc) has been extended to include target cells acutely infected with herpes simplex type 1 virus (hsv-1) or herpes simplex type 2 virus (hsv-2) in an in vitro system that employs immune human serum and human blood mononuclear cells. the cytotoxic reaction was detectable after 1 hr of incubation and was complete between 4 and 8 hr. the amount of adcc noted was directly proportional to the logarithm(10) of the effector: target cell ra ... | 1976 | 173757 |
| latent infection of sensory ganglia with herpes simplex virus: efficacy of immunization. | mice were used to test the efficacy of active immunization in preventing latent infection of local sensory ganglia that follows inoculation of superficial epithelial surfaces with herpes simplex virus. substantial but not complete protection was observed in animals immunized and challenged with herpes simplex virus type 1, but no protection was noted in animals immunized and challenged with herpes simplex virus type 2. latent ganglionic infection can develop in immunized animals despite the pres ... | 1975 | 166432 |
| depressed specific cell-mediated immunity to herpes simplex virus type 1 in patients with recurrent herpes labialis. | cell-mediated immunity to herpes simplex virus type 1 (hsv-1) was assessed by a lymphocytotoxicity 51-cr-release microassay procedure, using the ma-160 human prostatic adenoma cell line chronically infected with hsv-1 and its parent cell line as control. the specific immune release mean plus or minus standard deviation for nine asymptomatic patients with recurrent hsv-1 infections was 13.7 plus or minus 8.1%, compared to 28.9 plus or minus 8.4% in seven normal seropositive controls (p is less th ... | 1975 | 166930 |
| antibody patterns to herpesviruses in kaposi's sarcoma: serological association of european kaposi's sarcoma with cytomegalovirus. | sera from patients with kaposi's sarcoma (ks) were examined for antibody titres to cytomegalovirus (cmv), epstein-barr virus (ebv) and herpes simplex virus (hsv) types 1 and 2 by four techniques: indirect haemagglutination (iha), complement fixation (cf), virus neutralization (nt) and indirect immunofluorescence (if). the patients were classified, according to the stage of disease, as progressive and regressive. control sera were obtained from healthy adults, matched for age, sex, race, socioeco ... | 1975 | 166949 |
| polyamine metabolism in cells infected with herpes simplex virus. | ifection of ls cells with hsv-1 resulted in an inhibition of spermidine and spermine synthesis from putrescine, possibly through inhibition of host cell protein synthesis. the rate of putrescine uptake increased soon after infection, and later, polyamines were lost from the cells. inhibition of spermidine and spermine synthesis by methylglyoxal bis (amidinohydrazone) did not affect virus replication. | 1975 | 167106 |
| variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology. | this paper reports on the variability of structural polypeptides of 53 strains of herpes simplex virus 1 isolated from italy, uganda, south africa, and various locations in the united states. most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numberous passages at variable multiplicities in cell culture and experimental animals. the acrylamide gel electrophoresis of polypeptides from purified virions revealed seven ... | 1976 | 175016 |
| neuraminidase reversal of resistance to lysis of herpes simplex virus-infected cells by antibody and complement. | the susceptibility to lysis by antibody and complement was examined in four human cell lines. the cells were infected with herpes simplex virus type 1 and lysis was assessed by the 51cr release test by using antibodies to herpes simplex virus and guinea pig serum as a source of complement. the four cell lines were found to differ in their susceptibility to lysis, although virus replication was readily demonstrated in the different cell lines. by indirect immunofluorescence, no differences in the ... | 1976 | 175118 |
| selective inhibition of herpes simplex virus by 5-amino-2,5-dideoxy-5-iodouridine. | 5-amino-2,5-dideoxy-5-iodouridine, a nel thymidine analogue, is a potent inhibitor of herpes simplex virus type 1 replication. in contrast to most other nucleoside analogues which possess antiviral activity, 5-amino-2,5-dideoxy-5-iodouridine exhibits little, if any, cellular toxicity. preliminary evidence suggests that 5-amino-2,5-dideoxy-5-iodouridine selectively inhibits viral-specific dna synthesis. | 1975 | 167186 |
| presence of herpes simplex virus-related antigens in transformed l cells. | antiserum prepared against herpes simplex virus type 1 (hsv-1)-infected l cells, i.e., lytic antiserum, was shown by an indirect immunofluorescence test to stain 90 percent of hsv-transformed l or hela cells. immunofluorescence in these cells was always most intense in the perinuclear cytoplasmic region. similar results were obtained with antiserum prepared against hsv-transformed l cells. these data indicate that hsv-transformed cells (both l and hela) express hsv-related antigens. antiserum pr ... | 1975 | 167195 |
| anatomy of herpes simplex virus dna. ii. size, composition, and arrangement of inverted terminal repetitions. | electron microscope studies on self-annealed intact single strands and on partially denatured molecules show that herpes simplex virus 1 dna consists of two unequal regions, each bounded by inverted redundant sequences. thus the region l (70 percent of the contour length of the dna) separates the left terminal region a1b from its inverted repeat b'a'1, each of which comprises 6 percent of the dna. the region s (9.4 percent of dna) separates the right terminal region cas (4.3 percent of the dna) ... | 1975 | 167196 |
| inhibition by levamisole of metastases by cells transformed by herpes simplex virus type i. | levamisole was tested to determine whether the drug could reduce metastases by hsv-1-transformed cells in a model hamster system. the results presented reveal an inhibition of metastases to the lungs even when the drug is inoculated after development of subcutaneous tumors at the site of inoculation of the cells. | 1975 | 167374 |
| identification of strains of herpes simplex virus by comparison of the density of their dna using the preparative ultracentrifuge. | the buoyant densities of the dna of herpes simplex virus type 1, type 2 and pseudorabies virus, as determined in the analytical ultracentrifuge, are 1.725, 1.727 and 1.731 correlating with g+c contents of 67, 69 and 73 per cent respectively. the density differences for the dna's of type 1 and type 2 herpes simplex viruses have been confirmed in experiments with isotopically labelled dna from four type 1 and six type 2 strains by preparative cscl gradient ultracentrifugation. the dna of all the t ... | 1975 | 167692 |
| temperature-sensitive mutants of herpes simplex virus type 1 defective in lysis but not in transformation. | twelve temperature-sensitive (ts) mutants of herpes simplex virus type 1 (hsv-1), representing seven complementation groups, were isolated subsequent to 5-bromodeoxyuridine mutagenesis. these mutants were identified by their inability to replicate in a line of monkey (cv-1) cells at 39 c. seven of these mutants, representing six complementation groups, induced thymidine kinase (tk) and transformed ltk- cells, a line of mouse l cells lacking tk, to a tk+ phenotype at both the permissive (34 c) an ... | 1975 | 168402 |
| terminal repetitions in herpes simplex virus type 1 dna. | | 1975 | 169023 |
| analysis of dna of defective herpes simplex virus type 1 by restriction endonuclease cleavage and nucleic acid hybridization. | | 1975 | 169024 |
| viral gene functions expressed and detected by temperature-sensitive mutants of herpes simplex virus. | the expression of hsv-specific gene functions by 22 ts mutants of hsv-1 in 15 complementation groups and 8 ts mutants of hsv-2 in 7 complementation groups has been studied at the nonpermissive temperature. four cistrons of hsv-1 and three cistrons of hsv-2 with defects in viral dna and dan polymerase synthesis were identified. dna-mutants of hsv-1 revealed a greater alteration in hsv polypeptide synthesis and viral assembly than dna- mutants of hsv-2. mutants with apparent defects in structural ... | 1975 | 169028 |
| the expression of the syn- gene of herpes simplex virus type 1. i. morphology of infected cells. | | 1976 | 176783 |
| effects of cytochalasin b on herpes simplex virus type 1 replication. | | 1976 | 176798 |
| antibodies to herpes simplex virus in jewish women with cervical cancer and in healthy jewish women of israel. | herpes simplex virus (hsv) antibody titers were examined in sera from 39 jewish women with squamous cell carcinoma of the uterine cervix (cacx) and in sera from controls matched by age and country of origin. highly significant differences were found between the cases and controls for both hsv type 1 (hsv-1) and hsv type 2 (hsv-2). compared to findings in other demographic areas, the geometric mean titer (gmt) of hsv-1 among the cacx cases were considerably higher, whereas the gmt for hsv-2 was i ... | 1975 | 169365 |
| deoxyribonucleoside triphosphate pools in cells infected with deoxypyrimidine kinaseless herpes simplex virus. | deoxyribonucleoside triphosphate pools were analysed after infection of cells with mutant herpes simplex virus which lacks the ability to induce the enzyme deoxypyrimidine kinase. after infection of exponentially growing bhk c13 cells, an increase in all four dntp pools was observed. however, after infection of cells which themselves cannot incorporate exogenous pyrimidine deoxynucleosides only the purine deoxynucleoside triphosphate pools increased in size. in a system which is non-permissive f ... | 1976 | 177723 |
| the effect of tilorone hydrochloride on the growth of several animal viruses in tissue cultures. | tilorone hydrochloride, at a concentration of 10 mug/ml inhibits the growth of herpes simplex virus type 1 in bs-c-1 cells. the growth of vaccinia virus in bs-c-1 cells is partially inhibited; however, six viruses containing rna, including four members of the togavirus group grown in chick fibroblasts, are not affected by the drug. the inhibition of the growth of herpes virus by tilorone hydrochloride is greater when the multiplicity of infection is lower than 1 p.f.u/cell and when the drug is a ... | 1976 | 177724 |
| mutants of herpes simplex virus types 1 and 2 that are resistant to phosphonoacetic acid induce altered dna polymerase activities in infected cells. | three mutants of herpes simplex virus (hsv) have been isolated which form plaques in the presence of 100 mug/ml phosphonoacetic acid (ppa). all three mutants (3 from hsv-1 strain 17 syn+, 14 from hsv-1 strain 17 syn, and 19 from hsv-2) induce viral dna synthesis and viral dna polymerase activity, and these are much less sensitive to ppa than the wild-type virus. the results support the hypothesis that ppa interacts directly with the viral dna polymerase protein, at least part of which is virus c ... | 1976 | 177727 |
| dna synthesis and dna polymerase activity of herpes simplex virus type 1 temperature-sensitive mutants. | fifteen temperature-sensitive mutants of herpes simplex virus type 1 were studied with regard to the relationship between their ability to synthesize viral dna and to induce viral dna polymerase (dp) activity at permissive (34 c) and nonpermissive (39 c) temperatures. at 34 c, all mutants synthesized viral dna, while at 39 c four mutants demonstrated a dna+ phenotype, three were dna+/-, and eight were dna-. dna+ mutants induced levels of dp activity similar to thhose of the wild-type virus at bo ... | 1975 | 169388 |
| diagnosis of herpesvirus hominis infections in a general hospital laboratory. | there is a greatly increased interest in herpesvirus hominis infections especially those of type 2 associated with genital lesions or neonatal disease. physicians are eager to confirm the clinical impression with a specific virologic diagnosis such as isolation of the agent and its typing, and type-specific antibody responses. procedures are reviewed here which permit such studies in general microbiology laboratories equipped for simple cell culture and immunofluorescence. this paper recounts ex ... | 1975 | 170310 |
| genetic studies with herpes simplex virus type 1. analysis of mixed plaque-forming virus and its bearing on genetic recombination. | | 1975 | 163545 |
| spleen cell-mediated cytotoxicity of hamster cells transformed by herpes simplex virus: evidence for virus-specific membrane antigen. | the nature of the host's immune response to isografts of hamster embryo fibroblasts (hef) transformed by herpes simplex virus type 1 (hsv-1) was investigated by the microcytotoxicity assay. it was found that spleen cells from tumor-bearing hamsters killed homologous tumor cells but not hef transformed by cytomegalovirus or para-(defective sv40)-adenovirus 7 (para-7). cytotoxicity was lost as the tumor increased in size. spleen cells from animals bearing isografts of hsv type 2 (hsv-2) transforme ... | 1975 | 163859 |
| [herpes simplex virus antibodies in 18 patients submitted to immunosuppressive agents after renal transplantation]. | the titer of circulating antibodies to herpes simplex virus type 1 was tested in 18 patients for a few months before and after renal transplantation. no clinical illness was observed in the group of patients lacking antibodies. on the contrary, half of those with antibodies developed cutaneous eruption within 2 to 4 weeks following treatment with immunosuppressive drugs. no relationship could be found between the appearance of clinical illness and the rise in antibody titer. this lack of correla ... | 1975 | 172840 |
| anatomy of herpes simplex virus dna: evidence for four populations of molecules that differ in the relative orientations of their long and short components. | intact dna molecules extracted from hsv-1 (herpes simplex virus 1, human herpes virus 1) strain mp virions have a molecular weight of approximately 97 x 10(6), but cleavage with the hiniii restriction enzyme yields fourteen fragments with summed molecular weights of 160 x 10(6). six "major" fragments occur once in every molecule in the population and account for 60% of the genetic information. four "minor" fragments are present in amounts equivalent to one copy for every two genomes (0.5 molar r ... | 1975 | 172900 |
| induction and in vitro demonstration of cellular immunity to dna and rna viruses in guinea-pigs. | guinea-pigs were immunized with different cells infected with vaccinia virus, herpes simplex virus type 1, herpesvirus saimiri, and the virus of vesicular stomatitis. development of cellular immunity against these viruses was observed with transformation of blood and spleen lymphocytes and with the migration inhibition test using peritoneal exudate cells. cellular immunity against vaccinia virus was first seen 6 days after the inoculation of cell-bound vaccinia virus by lymphocyte transformation ... | 1975 | 173485 |