| metabolic characteristics of cells infected with a herpesvirus of turkeys. | chicken embryo fibroblasts infected with the nononcogenic herpesvirus of turkeys (hvt) displayed an increased rate of glucose uptake, a pronounced alteration of the ph of the medium, and an increased production of lactic acid when compared to mock-infected cultures. objective estimates of cytopathology (quantifiable neutral red uptake and cell protein determination) showed that cell deterioration was a slow process in hvt-infected cells when compared to infection by herpes simplex virus. experim ... | 1975 | 1548 |
| search for herpetic antibodies in the cerebrospinal fluid in senile dementia and mental retardation. | complement-requiring neutralizing antibodies to herpes simplex type 1 virus (hsv 1) in titres from 2 to greater than 16 were detected in the cerebrospinal fluid (csf) of 47% senile patients with various forms of dementia, but in none of mentally retarded adolescents and adults suffering from various neurologidal diseases. also the incidence of hsv 1 serum antibodies in elevated titres (larger than or equal to 512) was increased in senile demented patients (61%) as compared with persons in normal ... | 1975 | 1996 |
| isoelectric focusing of herpes simplex virus. | herpes simplex virus was inserted into a preformed stable ph gradient and electrofocused for about one hour. dextran was used as the density gradient forming agent. virions banded at ph 4.9+/-0.1 and nucleocapsids at 4.1+/-0.05. about 10-20 per cent of infective virus was recovered. | 1975 | 2144 |
| electron microscopy of virus particles in ultrathin frozen sections. | a procedure with the use of polyethylene glycol (peg) 600 to support biological materials for ultracryotomy was developed. developing and mature virions were demonstrated by electron microscopy in negatively stained frozen sections of vaccinia virus-infected human diploid cells and of herpes simplex virus (hsv) type 1 infected primary rabbit kidney cells. approximate localisation of the virus particles in the cells was possible. | 1976 | 5872 |
| effect of 9-beta-d-arabinofuranosylhypoxanthine 5'-monophosphate on genital lesions and encephalitis induced by herpesvirus hominis type 2 in female mice. | 9-beta-d-arabinofuranosylhypoxanthine 5'-monophosphate, administered orally or topically, increased the number of survivors and mean day of death in mice inoculated intravaginally with herpesvirus hominis type 2. topical application of the drug in ointment significantly reduced the severity of genital lesions. orally administered drug did not appreciably reduce genital lesions, but it did cause significant increases in the number of survivors and mean day of death, even when administration was s ... | 1976 | 6598 |
| biochemical classification of herpes simplex virus types 1 and 2, and of intermediate strains on the basis of different susceptibilities of thymidine kinase to thymidine analogues. | herpes simplex virus (hsv) type 1 can be differentiated from hsv type 2 on the basis of the sensitivity to 2'-deoxythymidine-5'-monophosphate of thymidine kinase induced in primary rabbit kidney cells. whereas thymidine kinase induced by five strains of hsv type 1 (tk 1) is stimulated by suitable concentrations of 2'-deoxythymidine-5'-monophosphate, thymidine kinase induced by eight strains of hsv type 2 (tk 2) is inhibited. on the other hand, tk 2 is strongly inhibited by 2'-deoxythymidine-5'-t ... | 1976 | 7938 |
| persistent infection of bhk cells with herpes simplex virus types 1 and 2 in the absence of specific anti-herpetic antibody. | persistent infection of bhk-21 cells with 5 strains of herpes simplex virus (hsv) type 1 and a 1 strain of hsv type 2 is described. acute infection only was obtained with two strains of type 1 and one strain of type 2. persistent infection could not be established in hela and rabbit lung cells. persistent infection of bhk cells proceeded in the absence of specific antibody. persistently infected cells were more resistant to superinfection with homologous or heterologous hsv as compared to infect ... | 1976 | 7939 |
| reactions of immune sera against the nucleocapsid, envelope and whole herpes simplex virus type 1. | antibodies against (a) naked particles, (b) virus envelope, and (c) whole herpes simplex virus (hsv) type 1 were investigated. immune rabbit serum against naked particles (and formalized naked particles) contained virus neutralizing (vn) antibody in a low titre as compared to the titre of complement-fixing (cf) antibody. immune rabbit serum against the viral envelope had similar titres of vn and cf antibodies. in gel double diffusion precipitation tests, whole hsv gave two precipitation zones wi ... | 1976 | 7940 |
| properties of dna ligase from uninfected and virus-infected hela cells. | hela cells contain a high m.w. form of dna ligase which can be completely converted to a low m.w. form. stokes radius, frictional ratio, sedimentation coefficient, molecular weight, ph dependence, and heat inactivation rate of the two forms have been studied. the major properties of the two forms of dna ligase in hela cells (in particular molecular weights and ph dependence) resemble those of the "dimer" and "monomer" structures described in cultured human cells (pedrali, g., spadari, s., ciarro ... | 1976 | 9621 |
| stable erythrocyte diagnostic preparation for passive haemagglutination test with herpes simplex virus antigen. | a method for the preparation of stable suspensions of erythrocytes sensitized with herpes simplex virus (hsv) antigen and for their use in the passive haemagglutination test (phat) was developed. formolized sheep erythrocytes were treated with tannin and sensitized with hsv antigen prepared from infected chick embryo cell culture by ultrasonication and virus extraction with alkaline glycine buffer. antibody titres determined in the phat were higher than titres of neutralizing antibody. the speci ... | 1976 | 11670 |
| survival of uv-irradiated herpes simplex type 1 virus in herpes simplex type 2-transformed hamster cells. | | 1976 | 11672 |
| increased titres of herpes simplex virus in rauscher leukosis-infected mice. | | 1976 | 11676 |
| ph mediated inhibition of the cell to cell spread of herpes simplex virus infection. | the relationships between the environmental ph and the replication and spread of herps simplex virus (hsv) infection in rabbit skin (rs) cell cultures was studies. relative to the plaque formation at ph7.0, incubation of rs cells with medium adjusted to ph 6.6 resulted in a 50 to 70 per cent decrement in plaque number. the addition of overlay media adjusted to ph 6.0 or 6.3 precluded hsv plaque formation. select hsv-1 (type 1) and hsv (type 2) strains readily survived a 3 day incubation with med ... | 1976 | 11763 |
| effect of ph on the kinetics of herpes simplex virus inactivation at 36 degrees. | | 1976 | 12611 |
| virus inactivation by grapes and wines. | infusions and extracts of different grapes inactivated poliovirus; agents responsible for this property resided in the skin of the grape. commercial grape juice at both natural and neutral ph inactivate various enteric viruses and herpes simplex virus; a 1,000-fold reduction in poliovirus infectivity occurred after incubation with grape juice, ph 7.0, for 24 h at 4 degrees c. a variety of wines were antiviral but to a lesser extent than grape juice; red wines were more antiviral than white. anti ... | 1976 | 12719 |
| herpes type-2 viruses and gynaecological malignancies. | numerous sero-epidemiologic studies have noted an association between herpes type 2 (ht-2) virus and carcinoma of the cervix. in a study to evaluate the role of this virus, if any, on the etiology of extra cervical pelvic malignancies in ibadan, the prevalence of ht-2 virus antibodies was found not to be significantly different in patients with extra cervical pelvic malignancies (carcinoma of the vulva and malignant trophoblastic disease) and cases of chronic cervicitis when compared with health ... | 1976 | 13005 |
| influence of host cell type on the density of herpes simplex virus particles. | the densities of purified herpes simplex virus (hsv) particles prepared from infected rabbit lung (zp) and baby hamster kidney (bhk-21) cells were investigated in potassium tartrate (pt) and potassium citrate (pc) density gradients. virions obtained from zp cells exhibited a higher density than those from bhk-21 cells. in pt and pc gradients, the former banded at densities of 1.226 and 1.267, while tha latter at 1.194 and 1.233, respectively. deenveloped viral particles prepared by nonidet p-40 ... | 1976 | 13640 |
| an analysis of factors influencing the isolation rate of herpes simplex virus. | attempts were made to improve the rate of isolation of herpes simplex virus (hsv) from clinical specimens by minimizing loss of virus infectivity during transportation and employing the most sensitive cells for isolation. basical analyses using standard strains of type 1 and type 2 hsv indicated that virus titer decrease was marked even at low temperatures in environments free of proteinous stabilizer such as normal serum or tissue extract, negating the generally held concept that hsv is stable ... | 1977 | 17816 |
| ribonucleotide reductase from herpes simplex virus (types 1 and 2) infected and uninfected kb cells: properties of the partially purified enzymes. | mammalian ribonucleotide reductase is a complex enzyme modified in its activity by a complex regulatory system involving adenosine triphosphate (atp) and deoxyribonucleoside triphosphates. infection of kb cells with herpes simplex virus (hsv) type 1 or 2 induces the formation of an altered ribonucleotide reductase. the properties of partially purified reductase from uninfected kb cells have been compared with the enzymes obtained from hsv-1 and hsv-2 infected kb cells. we found that the virus-in ... | 1977 | 18550 |
| syntheses of virus-induced thymidine kinase and viral dna in herpes simplex type 1 virus-infected chick embryo fibroblasts. | herpes simplex virus type 1, strain kupka, did not replicate in chick embryo fibroblasts (cef), but the infection was followed by the development of cytopathic changes. this effect could be abolished by uv irradiation of the virus. virus-induced thymidine kinase was synthesized in the infected cells reaching a maximum level at 24 hours post infection (p.i.). in the presence of cytosine arabinoside, thymidine kinase synthesis was enhanced. this suggested that the late (post-replicative) viral fun ... | 1977 | 18914 |
| production and some properties of neutralizing antigens of herpes simplex virus. | human diploid cells (lep) infected with herpes simplex virus (hsv) type 1 were extracted by treatment with nonidet p-40. the content of antigens reactive with neutralizing antibody in the clarified extract was measured by the blocking test. influence of various factors on the production of the blocking antigens (ba) was tested. highest ba titres in lep cells were achieved at a multiplicity of infection (m.o.i.) of 0.5 tcd50/cell, medium 199 and 22 to 29 hours incubation at 37 degrees c. the use ... | 1977 | 18916 |
| nonstructural proteins of herpes simplex virus. i. purification of the induced dna polymerase. | herpes simplex virus-induced dna polymerase purified by published methods was found to be contaminated with many others proteins, including virus structural proteins. thus, deae-cellulose and phosphocellulose chromatography were used in combination with affinity chromatography to purify dna polymerase from herpes simplex virus type 1- and type 2-infected cells. the purified enzyme retained unique features of the herpesvirus-induced dna polymerase, including a requirement for high salt concentrat ... | 1977 | 21304 |
| measurement of antibodies to herpesvirus types 1 and 2 in human sera by microradioimmunoassay. | the binding of 125i-labelled anti-human antibodies against the fc igg fragment to unlabelled antiviral immunoglobulins in the surface of infected cells was used to quantitate antibodies against herpes simplex virus type (hsv-1) and type 2 (hsv-2) in sera from patients with cervix carcinoma. the microradioimmunoassay technique (micro-ria) proved to be 5-10 times more sensitive than the microneutralization test. antibody titres determined by micro-ria correlated with neutralizing antibody titres t ... | 1977 | 22235 |
| some properties of the adenosine triphosphatase associated with herpes simplex virus and nuclear membrane of host cells. | an enzyme capable to split adenosine triphosphate (atp) was shown to be firmly associated with mature herpes simplex virus particles purified from infected rabbit lung (zp) cells. the enzyme localized in the viral envelope was markedly activated by bivalent cations, to the largest degree by mg2+ at a ph optimum of 7.8--8.0. na+ and k+ ions neither separately nor together showed any activating effect. enzyme activity was not sensitive to the action of ouabain. no adenosine diphosphatase (adpase) ... | 1978 | 25004 |
| increased susceptibility to infection with herpes simplex virus types 1 and 2 of cold-adapted l cells. | l cells (l-as subline) have been adapted to a temperature of 4 degrees c. in the cold-adapted cells, designated lc3, greater amounts of infectious herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) were synthesized than in the original l-as cells or in another control l-cell line. two strains of hsv-1 reached higher infectious titres in lc3 cells grown at 36 degrees c than in those grown at 32 degrees c. the hsv-2 strain tested replicated in lc-3 cells grown at 32 degrees c better than at higher ... | 1978 | 26196 |
| multiplication of herpes simplex virus types 1 and 2 in macrophages of nmri and c57/bl mice. | the multiplication of 8 strains of herpes simplex virus of type 1 and 2 in normal and stimulated macrophages of nmri and c57/bl mice was tested. only 3 strains multiplied in normal nmri macrophages, whereas all strains multiplied in stimulated cells. no multiplication except of one strain was observed in c57/bl macrophages. the multiplication rates of the virus strains correlated well with the determination of the acid-soluble and acid-insoluble thymidine (dtr) radioactivity and autoradiographic ... | 1978 | 27966 |
| failure of 5-bromodeoxyuridine to influence the activation of latent herpex simplex virus infection in explants of the rabbit regional sensory ganglia. | the occurence of virus-specific antigens in explants of the gasserian ganglia of rabbits with healed keratitis induced by the kupka strain of human herpesvirus type 1 was tested by indirect immunofluorescence. in explants, the neurons and satellite cells revealed specific fluorescence from the 3rd day of culturing. the pattern of virus activation in the ganglion tissue was similar irrespective of whether the explants were cultured in the presence or absence of 5-bromo-2'-deoxyuridine. | 1978 | 27976 |
| experimental pathogenesis of non-lethal herpesvirus infection and the establishment of latency. | in rabbits inoculated into the right scarified cornea with the kupka strain of human herpesvirus type 1 (hhv 1), healing of the keratitis occurred between days 8--12 post infection (p.i.). viral particles were seen in nuclei and cytoplasm of corneal epithelium cells and in a few fibrocytes of corneal stroma. by immunofluorescence and electron microscopy, the virus was demonstrated in single neurons and satellite cells of the homolateral gasserian ganglion since 24 hr p.i. from 20 hr to 8 days p. ... | 1978 | 29466 |
| photosensitization of herpes simplex virus type 1 with neutral red. | commercial neutral red (nr) originally containing at least 8 components was purified by thin layer chromatography. herpes simplex virus type 1 (hsv-1) treated in vitro with 30 microgram/ml of purified nr became sensitive to light inactivation within 2 min but rapidly lost this sensitivity upon dilution. similarly, virus grown in the presence of nr lost its photosensitivity upon dilution of the virus stock. in both cases the kinetics of inactivation appeared to be multi-hit. photoinactivation of ... | 1978 | 31414 |
| practical aspects of the storage of herpes simplex virus samples. | | 1979 | 34257 |
| influence of experimental influenza infection of the eye on the course of herpetic keratitis in rabbits. | influenza virus monoinfection and combined influenza and herpes simplex virus (hsv) infection of the eye was studied in rabbits. influenza a/hong kong/1/68 (h3n2) virus caused a clinically overt disease of the eye only after inoculation into the eye chamber. the combined influenza and hsv infection of the eye induced severe iridocyclitis. the combined infection of the eye with influenza virus and hsv had a more severe course than hsv monoinfection. the occurence of influenza antibody in the lacr ... | 1978 | 35945 |
| viral superinfection in cells carrying an arenavirus and/or a togavirus. | four lines of the same l-cell clone were transferred 60 times in parallel: uninfected cells, a line carrying lymphocytic choriomeningitis virus (lcmv), another one carrying tick-borne encephalitis virus (tev) and one carrying both viruses. in double persistency, lcm and tev were suppressed and stimulated, respectively. cell multiplication rates were comparable in all four lines. single lcmv persistence caused marked resistance of l cells to superinfecting viruses from various taxonomic groups, b ... | 1978 | 35946 |
| effects of cytosine arabinoside on the incorporation of pyrimidine nucleosides into hamster cells transformed by herpes simplex type 1 and type 2 viruses and by human cytomegalovirus. | the incorporation of 3h-thymidine and 3h-deoxycytidine into acidoprecipitable fraction of hamster cells transformed by herpes simplex viruses type 1 and type 2 and of 3h-thymidine into hamster cells transformed by human cytomegalovirus was found to be resistant to the action of cytosine arabinoside. more 3h-thymidine was incorporated into these cells in the presence than in the absence of the drug. similar stimulaton of 3h-thymidine uptake could be achieved by using unlabelled deoxycytidine inst ... | 1979 | 40418 |
| influence of environmental ph on the preservation and inactivation of herpes simplex virus. | | 1979 | 41259 |
| analysis of herpes simplex virus isolated from patients with recurrent herpes keratitis exhibiting "treatment-resistance" to 5-iodo-2'-deoxyuridine. | four strains of herpes simplex virus (hsv) were isolated from two patients with recurrent herpes keratitis who failed to respond to 5'-iodo-2'-deoxyuridine (idu) treatment. two of the four isolates were highly resistant to idu in cell culture and the other two isolates were more susceptible to idu than an hsv-1 laboratory strain. from each patient, an idu-resistant and an idu-susceptible virus was isolated. all 4 isolates possessed the ability to induce the thymidine kinase (tk) activity in cell ... | 1979 | 41435 |
| latent herpesvirus hominis 1 in the central nervous system of psychotic patients. | cerebrospinal fluids (csf) from 35 patients with senile or presenile dementia and from 13 patients with schizophrenia and related syndromes were examined in cell cultures with the aim to isolate herpesvirus hominis 1 (hvh 1) or other viruses. serum and csf antibodies to hvh 1 and/or interferon in the patients indicated a recent hvh 1 antigenic or viral activity. in the csf of two senile demented patients and of one patient with schizoaffective psychosis, agents of low virulence, causing a cytopa ... | 1979 | 41436 |
| development of tumours in mice chronically infected with herpes simplex virus. | tumours developed in chronic infection lasting for 150--180 days in 39 (60%) of 65 mice infected with strain l2 of herpes simplex virus (hsv) type 1 and in 12 (20%) of 60 mice infected with strain 333 of hsv type 2. similar results were obtained in 150 immunosuppressed mice chronically infected with hsv types 1 and 2. pathomorphologically, the neoplasias in the first group (strain l2) were similar to adenocarcinoma and malignant lymphoma and in the second (strain 333) to lymphoma and angio- or f ... | 1979 | 42295 |
| effect of 2-deoxy-d-glucose on the binding of preimmune and immune sera to herpes simplex virus-infected cells as measured by quantitative immunofluorescence. | the binding of immune and preimmune rabbit sera to fixed vero cells infected with herpes simplex virus type 1 (hsv 1) was measured by quantitative immunofluorescence. false positive binding of the preimmune serum (ps) occurred from 8 hr post infection (p.i.), while the immune serum (is) revealed positive fluorescence starting with 4 hr p.i. the false binding of ps decreased significantly (p = 0.01) if the cells were cultured in the presence of 2-deoxy-d-glucose (dog). statistical analysis of dat ... | 1979 | 42300 |
| role of fc receptors in herpes simplex virus infection. | it is suggested that fc receptors which appear on the surface of cells infected by herpes-simplex virus confer a biological advantage on the virus--perhaps by binding immunoglobulin molecules or antigen-antibody complexes on the cell surface, thus blocking viral antigenic sites, or by consuming antibody directed at surface viral antigen or by modifying replication of the virus. | 1975 | 46027 |
| effect of cytosine arabinoside on viral-specific protein synthesis in cells infected with herpes simplex virus. | the relationship between viral dna and protein synthesis during herpes simplex virus type 1 (hsv-1) replication in hela cells was examined. treatment of infected cells with cytosine arabinoside (ara-c), which inhibited the synthesis of hsv-1 dna beyond the level of detection, markedly affected the types and amounts of viral proteins made in the infected cell. although early hsv-1 proteins were synthesized normally, there was a rapid decline in total viral protein synthesis beginning 3 to 4 h aft ... | 1975 | 46282 |
| lifetimes of mrna molecules directing the synthesis of viral proteins in herpes simplex virus-infected cells. | possible variations in the functional lifetimes of herpes simplex virus type 1 mrna molecules in infected hela cells were studied. as shown by the rate of decrease of radioactive amino acid incorporation into viral proteins after the addition of actinomycin, the average lifetime of early viral mrna's are shorter than those for the late messenger species. in addition, when the viral proteins made after the addition of actinomycin were further analyzed by gel electrophoresis, it was found that mes ... | 1975 | 46283 |
| immune specific production of interferon by human t cells in combined macrophage-lymphocyte cultures in response to herpes simplex antigen. | human peripheral blood lymphocytes, highly enriched for t cells, were obtained by passing gravity-sedimented leukocytes through nylon wool columns. the eluted cells were cultured with autologous macrophages and the mixture was studied for its capacity to produce interferon in vitro in response to stimulation with herpes simplex virus antigen. the interferon produced by the combined macrophage-lymphocyte cultures was shown to depend upon the presence of t cells; elimination of these cells by trea ... | 1975 | 46881 |
| differential feulgen-deoxyribonucleic acid hydrolysis patterns of herpes simplex virus type 1 and type 2 infected cells. | infection of human embryonic lung cells with herpes simplex virus type 1 (hsv-1) and herpes simplex type 1 (hsv-2) resulted in: (a) qualitative (nuclear cytopathologic) alterations and quantitative (nuclear area) differences in infected compared to control nuclei; (b) increased feulgen-deoxyribonucleic acid (f-dna) amounts in infected cells, probably due to viral dna; (c) higher f-dna levels in hsv-2 infected cells; and (d) increased rates of f-dna hydrolysis in viral-infected as compared to uni ... | 1975 | 47871 |
| [herpes simplex virus and the nervous system]. | | 1975 | 49029 |
| herpes simplex virus type 1 replication in a gingival cell line. | propagation of herpes simplex virus type 1 (hsv-1) in gingival cells is advantageous since these cells are more definitive than hela cells in demonstrating viral-induced cytopathic effects and are easily and economically maintained in the laboratory. they also provide a potential medium for studying oral tissue reactions to hsv-1 infection. hsv-1-induced cytopathic effects are clearly characterized following inoculation with virus from standard laboratory cultures and human infections. | 1975 | 49366 |
| letter: immunopotentiation by levamisol. | | 1975 | 49491 |
| immunological basis for latency, recurrences and putative oncogenicity of herpes simplex virus. | the development of latency and recurrent infection after primary herpes simplex virus (h.s.v.) infection can be interpreted in terms of cell-mediated and antibody responses to virus-specific antigens and fc receptors on the surface of the infected cells. primary infection will induce immune responses to the virus, and antibody and cell-dependent cytotoxic mechanisms will kill most of the virus and virus-infected cells which are accessible to killer cells. h.s.v. will be sequestrated to the nerve ... | 1975 | 49654 |
| letter: rapid diagnosis of virus infections. | | 1975 | 49678 |
| antigenic specificity of s.m.o.m. virus by indirect immuno-fluorescent test. | | 1975 | 50056 |
| use of polyethylene glycol-treated calf serum for cell cultures in virus and interferon studies. | immunoglobulins and lipoproteins can be efficiently removed from calf serum by precipitation with polyethylene glycol (peg) 6000 under sterile conditions. the peg-treated serum is suitable for cell cultures used for virus growth and assays. moreover, peg was found to slow down the growth of l cells and to enhance the production and activity of mouse interferon. | 1975 | 50724 |
| shedding of infectious virus/antibody complexes from vesicular lesions of patients with recurrent herpes labialis. | the concentration of herpes-simplex virus (h.s.v.) in the lesions of adults with recurrent herpes labialis was determined. on the 1st day the vesicle appeared, the fluid within the lesion contained 10(5-3) plaque-forming units (p.f.u.) of h.s.v./mul. by swabbing the surface of the lesions with a sterile pledget, 10(6-2) p.f.u. of virus was isolated from the inflamed labial mucosa. the amount of virus obtained from the labial surface decreased on the 2nd and 3rd day to 10(5-0) and 10(3-0) p.f.u., ... | 1975 | 51344 |
| mode of inhibition of herpes simplex virus dna polymerase by phosphonoacetate. | phosphonoacetate is a highly specific inhibitor of herpes simplex virus-induced dna polymerase. sensitivity of herpesvirus type 1 or type 2 induced dna polymerase to the drug was similar. however, dna polymerases from other sources such as the host cells (wi-38), micrococcus luteus, and hepatitis b virus were highly resistant. in addition, escherichia coli rna polymerase and reverse transcriptase of rous sarcoma virus were also insensitive to the drug. enzyme kinetic studies showed that inhibiti ... | 1975 | 53071 |
| antibodies to herpes-simplex virus in "normal" cerebrospinal fluid. | thirty samples of cerebrospinal fluid (c.s.f.) obtained during routine myelography were examined for antibodies against herpes-simplex virus (h.s.v.) by antibody-mediated cell-dependent immune lysis. antiviral antibody was found in the c.s.f. of all ten patients who were subject to recurrent cold sores. thirteen of the twenty patients who denied or could not recall any previous herpetic infection had a serum antibody, and nine of them also had c.s.f antibody. all patients with c.s.f. antibody al ... | 1976 | 54583 |
| excretion of specific glycoproteins by cells infected with herpes simplex virus, types 1 and 2. | | 1975 | 54930 |
| biochemical and serological properties of the thymidine-phosphorylating enzymes induced by herpes simplex virus mutants temperature-dependent for enzyme formation. | | 1976 | 54980 |
| serological specificity of thymidine kinase activity in herpes simplex virus-transformed l cells. | | 1976 | 54981 |
| mechanism of phosphonoacetate inhibition of herpesvirus-induced dna polymerase. | phosphonoacetate was an effective inhibitor of both the marek's disease herpesvirus- and the herpesvirus of turkey-induced dna polymerase. using the herpesvirus of turkey-induced dna polymerase, phosphonoacetate inhibition studies for the dna polymerization reaction and for the deoxyribonucleoside triphosphate-pyrophosphate exchange reaction were carried out. the results demonstrated that phosphonoacetate inhibited the polymerase by interacting with it at the pyrophosphate binding site to create ... | 1976 | 55273 |
| an alternative theory of herpes-simplex recurrence and a possible role for prostaglandins. | reactivation of herpes-simplex is thought to require stimulation of latent virus in the sensory ganglion before the virus travels to the skin and causes a lesion. an alternative theory is proposed whereby virus is often released from the ganglion to form microfoci of infection in the skin but these are usually eliminated. physiological changes in the skin, perhaps induced by prostaglandins, occasionally allow lesions to develop. | 1976 | 55654 |
| letter: herpes-simplex encephalitis. | | 1976 | 55767 |
| letter: herpes-simplex-virus antigens in normal cervical cells. | | 1976 | 55882 |
| in vivo and in vitro measurements of the relationship of human squamous carcinomas to herpes simplex virus tumor-associated antigens. | an additional 244 unfiltered sera have now been studied in a series of controlled, coded tests to determine the relationship of squamous carcinomas of the head and neck and cervix to the presence of complement-fixing antibodies to herpesvirus-tumor-associated antigens (hsv-taa) in both tumor-bearing and cured patients. ninety % of sera from patients with squamous carcinomas had antibodies to hsv-taa, in contrast to 11% of sera from patients with nonsquamous cancers and 4% of sera from noraml ind ... | 1976 | 56226 |
| serological and epidemiological considerations of the role of herpes simplex virus type 2 in cervical cancer. | to assess the possible biological significance of the observations that women with cervical cancer tend to be younger at first intercourse than control women, data from 1823 women were analyzed for the relationship between age at 1st intercourse and number of sex partners. women who were younger at first intercourse had more sex partners than did women who were older at first intercourse. the interdependence of age at first intercourse and number of sex partners does not exlude the possibility t ... | 1976 | 56227 |
| herpes simplex vaccines. | prospects for the development of vaccines against herpes simplex virus types 1 and 2 and other herpesvirus group vaccines are discussed from the scientific, medical, and economic standpoints. probably, the most practical development will be glycoprotein subunit vaccines, prepared from herpes simples virus types 1 and 2, that will be tested for prophylaxis and therapy of acute viral disease and for cancer that may be caused by these agents. | 1976 | 56228 |
| the association of the infecting dna of herpes simplex virus with cellular components. | | 1976 | 56811 |
| [etiologic therapy of virus stomatitis]. | | 1976 | 57655 |
| cell-mediated immune response to herpes simplex virus: type-specific lymphoproliferative responses in lymph nodes draining the site of primary infection. | cell-mediated immune responses to type 1 and type 2 hsv were studied in rabbits using an in vitro lymphocyte transformation assay. the footpad route of inoculation was used to allow us to study the specificity and degree of localization of the responses. rabbits inoculated in the hind footpads with infectious hsv-i or hsv-ii mount type-specific lymphocyte transformation responses that are localized to draining lymphoid organs. type-specificity requires careful control of all in vitro culture con ... | 1976 | 58037 |
| in vivo and in vitro "markers" of human cervical intraepithelial neoplasia. | the human uterine cervix offers a unique opportunity to study the early lesions of squamous cell carcinoma, i.e., carcinoma in situ and dysplasia [combined as cervical intraepithelial neoplasia (cin)]. in vivo, the patients with cin have the epidemiological common denominators or "markers" of early onset of coitus, multiple sexual partners, 1st delivery before age 20, and antibodies to herpes simplex virus type 2 more frequently than do controls. the lesions themselves have specific epithelial a ... | 1976 | 58720 |
| basophil-sensitizing antibody response to herpes simplex viruses in rabbits. | antibodies to herpes simplex virus type 1 and type 2 were detected in the sera of rabbits by release of histamine from basophils sensitized in vitro with the sera. the time course of the appearance of the antibodies, the dose-response curve of the release of histamine in relation to antigen concentration, the sedimentation characteristics of the antibodies in sucrose gradients, and the ability to destroy the sensitizing capacity of the sera with heat suggest that the antibodies being assessed we ... | 1976 | 58935 |
| quantitation of antibodies to herpes simplex virus types 1 and 2 by complement-dependent antibody lysis of infected cells. | the release of 51cr from cells infected with herpes simplex virus type 1 or type 2 by antibody and complement was examined as a method of quantitating antibodies to the viruses. with decreasing concentration of antibody, a typical dose-response curve was observed; a region of antibody excess in which dilution did not affect percentage of specific 51cr release followed by a region in which a linear relation existed between dilution and percentage of specific 51cr release. therefore, a quantitativ ... | 1976 | 60057 |
| relative concentrations in human sera of antibodies to cross-reacting and specific antigens of herpes simplex virus types 1 and 2. | the relative concentrations of antibodies to the cross-reacting and type-specific antigens of herpes simplex virus types 1 and 2 were examined in sera from patients who had been infected by type 1 virus only, by type 2 virus only or by both viruses. complement-dependent lysis of 51cr-labeled cells was used to measure antibodies to the surface antigens of cells infected by the viruses. approximately 80% of the antibody activity was attributable to cross-reacting antigens and 20% to type-specific ... | 1976 | 60058 |
| oncogenic viruses: implications for human diseases (part ii). | | 1976 | 60244 |
| herpes-simplex-type-2-antibodies and hla-b12 in cervical cancer. | | 1976 | 61533 |
| latency in herpesvirus hominis: its relationship to oncogenesis and recurrent disease. | | 1976 | 61629 |
| a study of possible biohazards in the fluorescent antibody test using adenovirus, coxsackievirus, herpesvirus, and respiratory syncytial virus as antigens. | infectious adenovirus type 5 and coxsackievirus type b5, both nonlipid-containing viruses, were isolated from cells fixed in acetone at 22 degrees c for 15 min, from acetone used for fixation, from the solution used for washing slides during the fluorescent antibody procedure, and after complete processing of antigen preparations with serial twofold dilutions of human antisera and fluorescein-labeled goat anti-human immunoglobulin g. lipid-containing herpes simplex virus type 1 and respiratory s ... | 1976 | 61971 |
| herpes simplex virus type 2 and cancer of the prostate. | a seroepidemiologic study was performed to determine if there was an association between antibodies to herpes simplex virus type 2 and cancer of the prostate, similar to that reported for herpes simplex virus type 2 and cervical carcinoma. no significant difference in herpes simplex virus type 2 antibodies was seen between patients with cancer of the prostate and those with benign prostatic hypertrophy. further studies are needed to define the possible role of herpes simplex virus type 2 in the ... | 1976 | 62063 |
| clinical and virological studies on genital herpes. | | 1976 | 62191 |
| serum antibodies to herpesvirus early antigens in patients with cervical carcinoma determined by anticomplement immunofluorescence technique. | sera from patients with carcinoma of the uterine cervix were used with the anticomplement immunofluorescence (acif) technique to develop a simple procedure for detection of antibodies to herpes simplex virus type 2 (hsv-2) early antigens. test cells used in the acif assay were hep-2 cells infected with hsv-2 sequentially treated with inhibitors of protein and of dna synthesis. the cells were first treated for 7 h with cycloheximide (100 mug/ml) and then for 3 h with hydroxyurea (150 mug/ml). in ... | 1976 | 62718 |
| virological studies in genital herpes. | | 1976 | 62932 |
| recent advances in the study of cerebrospinal fluid. | | 1976 | 63121 |
| [method of study of conjunctival scrapings for diagnosis of mixed viral infection of the anterior portion of the eye]. | | 1976 | 63643 |
| detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their specificity. | skin fibroblasts exposed to cytosine arabinoside (ara c) were infected with either cytomegalovirus (cmv) or herpes simplex virus (hsv) type 1 and 2. herpesvirus-determined early antigens (hv-ea), detected by anti-complement immunofluorescence (acif), occurred primarily in the nucleic, and the specificity of these results was established by an acif blocking reaction using f(ab')2 fragments of human and hyperimmune reference sera. direct tests with selected sera and cross-blocking experiments betw ... | 1977 | 64461 |
| expression of an early, nonstructural antigen of herpes simplex virus in cell transformed in vitro by herpes simplex virus. | hyperimmune rabbit antiserum to an early, nonstructural herpes simplex virus type 2 (hsv-2)-induced polypeptide (vp143) reacted in immunofluorescence tests with a variety of cell lines transformed by hsv-2. cytoplasmic fluorescence was observed in 10 to 50% of hsv-2-transformed cells, whereas no fluorescence was observed in cells transformed by other oncogenic dna viruses or by a chemical carcinogen. vp143-specific reactivity could be absorbed from anti-vp143 serum with hsv-2-transformed cells b ... | 1977 | 64626 |
| herpes-simplex antigen in rabbit cerebrospinal fluid. | | 1977 | 64687 |
| impairment of immunity during pregnancy and antiviral effect of amniotic fluid. | in 297 pregnant women serum-levels of antibody to rubella virus were determined by haemagglutination inhibition, and serum-levels of antibodies to herpes-simplex virus and toxoplasma gondii were measured by indirect immunofluorescence tests. sera of age-matched non-pregnant women with intrauterine devices were also tested for the antibodies. a lower level of humoral immunity was found in the pregnant women than in the controls, and this fell further as pregnancy progressed. investigation of seru ... | 1977 | 64858 |
| regulation of herpesvirus thymidine kinase activity in lm(tk) cells transformed by ultraviolet light-irradiated herpes simplex virus. | | 1977 | 65051 |
| expression of type-specific virion structural antigen(s) in l cells transformed by herpes simplex virus types 1 and 2. | | 1977 | 65052 |
| glycoproteins specified by herpes simplex virus type 1: their synthesis, processing and antigenic relatedness to hsv -2 glycoproteins. | | 1975 | 65316 |
| immunological and biochemical characterization of polypeptides induced by herpes simplex virus types 1 and 2. | | 1975 | 65317 |
| the mononuclear cell in human blood which mediates antibody-dependent cellular cytotoxicity to virus-infected target cells. ii. identification as a k cell. | studies were carried out to determine whether the mononuclear cell in human blood which mediates antibody-dependent cellular cytotoxicity (adcc) to herpes simplex virus (hsv)-infected target cells has surface fc receptors which participate in the reaction. the f (ab')2 fragment of human igg antibody was inactive both in adcc and in complement-mediated cytolysis, but retained the capacity to neutralize infectious virus, to agglutinate erythrocytes coated with viral antigens, and to bind to the su ... | 1977 | 65428 |
| jejunal viral antigen in multiple sclerosis and amyotrophic lateral sclerosis. | | 1977 | 65554 |
| cell-mediated immunity to herpes simplex virus types 1 and 2 antigens in leukoplakia and carcinoma in man. | cell-mediated immune responses to herpes simplex virus 1 and type 2 virion and non-virion antigens were assessed in patients and in controls with oral leukoplakia, carcinoma and recurrent herpes labialis. enhanced proliferation was found in lymphocytes from patients with recurrent herpes labialis or with leukoplakia showing epithelial atypia, and depressed responses were found in carcinoma. very significantly positive correlations were shown between the responses to each of the herpes virus anti ... | 1976 | 65753 |
| viral-specific thymidine kinase in sensory ganglia of mice infected with herpes simplex virus. | | 1977 | 65830 |
| preventing the spread of herpes genitalis. | | 1977 | 66612 |
| an immunofluorescence technique with counterstain on fixed cells for the detection of antibodies to human herpesviruses; antibody patterns in patients with hodgkin's disease and nasopharyngeal carcinoma. | an indirect immunofluorescence (if) test on fixed cells with evans' blue counterstain is described for all four human herpesviruses, i.e., herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2), varicella-zoster virus (vzv), cytomegalovirus (cmv) and epstein-barr virus (ebv). comparison with immunodiffusion (id) for hsv-2 and with id and complement fixation (cf) for vzv and cmv demonstrated the specificity and high sensitivity of the if test. also introduced is a modification of the anti-compl ... | 1976 | 67099 |
| enhancement of ige-mediated histamine release from human basophils by viruses: role of interferon. | human leukocytes maintained in culture are induced to release histamine when exposed to ragweed antigen e or anti-ige. leukocyte cultures incubated with virus (i.e. hsv-1, influenza a, and adeno-1) but not exposed to ragweed antigen e or anti-ige fail to release histamine. if, however, leukocyte cultures are first exposed to virus and then to ragweed antigen e or anti-ige, significant enhancement of histamine release occurs. both infectious and inactivated virus enhance histamine release and the ... | 1977 | 67173 |
| occurrence of genital herpes simplex and cytomegalovirus infections in pregnancy. | a total of 244 pregnant women, 172 in the first and 72 in the third trimester, were screened for genital herpes simplex (hsv) and cytomegalovirus (cmv) infections using virus isolation, cytological examination and serological studies. in addition, immuno-fluorescence staining of exfoliated cervical cells was used for the detection of hsv infections. herpes simplex virus (hsv) could not be isolated and no characteristic hsv altered cells were found in cytological (papa) smears. cytomegalovirus wa ... | 1977 | 67744 |
| jejunal immunopathology in amyotrophic lateral sclerosis and multiple sclerosis. identification of viral antigens by immunofluorescence. | jejunal mucosa from 7 patients with amyotrophic lateral sclerosis (a.l.s.), 20 newly reported patients with multiple sclerosis (m.s.), and 35 control patients without either disease was studied by immunofluorescence. an immune reaction was present in all a.l.s. specimens and consisted of altered ratios of immunoglobulin-labelled cells in the lamina propria, complement-labelled cells in the same location, and, in some, immunoglobulin and complement deposits in the epithelial basement membrane. po ... | 1977 | 68222 |
| the role of t cells in anti-herpes simplex virus immunity. i. induction of antigen-specific cytotoxic t lymphocytes. | mice infected with herpes simplex virus develop little or no cytotoxic t lymphocyte (ctl) response. however, in lymph nodes (ln's) draining a local site infected with hsv, antigen-specific ctl precursors are sensitized, which upon transfer to in vitro culture conditions develop within 72 hr into effective ctl. the in vivo blockade of ctl differentiation can be overcome by cyclophosphamide, suggesting that a cyclophosphamide-sensitive mechanism blocks the in vivo generation of hsv-immune ctl. the ... | 1977 | 70478 |
| activation of an endogenous c-type rna virus in rat embryo cells after transformation by herpes simplex virus types 1 and 2. | reverse transcriptase activity was detected in the supernatants of rat embryo fibroblast cell cultures transformed by hsv types 1 and 2 at either the sub-optimal temperature of 20 degrees c or the supra-optimal temperature of 42 degrees c. rat cells clones which had been transformed at 20 degrees c contained higher levels of c-type virus dna polymerase than did cell clones which had been transformed at 42 degrees c. syncytia formation typical for c-type rna viruses occurred at passages higher th ... | 1977 | 70508 |
| detection of herpes simplex virus-related antigens in the nuclei and cytoplasm of biochemically transformed cells with peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence. | herpes simplex virus (hsv)-related antigens have been demonstrated in the nuclei and cytoplasm of human and mouse cells biochemically transformed by ultraviolet light-irradiated hsv. this was accomplished by using peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence with rabbit antisera that had high neutralizing titers against the hsv-specific thymidine kinase activity and virus infectivity. hsv-1 antisera reacted with antigens in cells biochemically transformed by ... | 1977 | 71279 |